Effect of dietary L-arginine supplementation on the expression of vascular endothelial growth factor A, its receptors, and nitric oxide system components in the pancreas of streptozotocin-induced diabetic rats

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-08-05 DOI:10.1016/j.tice.2025.103072

Duygu Yaman Gram , Meryem Şentürk , Gonca Kamacı Özocak , Nesrin Delibaşı Doğan , Görkem Ekebaş , Ayhan Atasever , Meryem Eren

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引用次数: 0

Abstract

T1DM is characterized by elevated blood glucose levels, insulin insufficiency, and alterations in vessel formation. Therefore, the expression of insulin, active caspase-3, inflammatory factors such as TNF-α and NF-κB, and components of the NO and VEGFA systems were investigated in the pancreas of STZ-induced diabetic rats. Blood glucose levels and body weight were also measured. Additionally, the effects of L-arginine supplementation on the same parameters were evaluated. The body weight of the STZ and STZ + L-arginine groups was lower than that of the control and L-arginine groups. L-arginine supplementation reduced blood glucose levels and increased insulin levels in diabetic rats. Degenerative changes in β-cells were observed in the pancreas of the diabetic group. Insulin expression was low in the STZ-group, while it was higher in the STZ + L-arginine group. VEGFA was localized in the islets, epithelial cells of glands, tubules, and endothelial cells in both the control and L-arginine groups. A similar localization pattern was observed for VEGFA in the STZ and STZ + L-arginine groups. Expression of eNOS and VEGFR2/FLK1/KDR was detected in the islets of the control and L-arginine groups. Their expression was low in the STZ and STZ + L-arginine groups. However, VEGFR2/FLK1/KDR signal intensity was stronger in STZ + L-arginine group than in the STZ group. The expression levels of iNOS, active caspase-3, TNF-α, and NF-κB were significantly higher in STZ group than in the control group.

In conclusion, although L-arginine treatment reversed STZ-induced hyperglycemia and insulin deficiency, it did not exert a significant effect on the expression levels of iNOS, active caspase-3, TNF-α, or NF-κB. Additionally, L-arginine appears essential for islet vessel maintenance by increasing VEGFR2/FLK1/KDR expression.

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膳食补充l -精氨酸对链脲佐菌素诱导的糖尿病大鼠胰腺血管内皮生长因子A及其受体和一氧化氮系统组分表达的影响

T1DM的特征是血糖水平升高、胰岛素不足和血管形成改变。因此，我们研究了stz诱导的糖尿病大鼠胰腺中胰岛素、活性caspase-3、炎症因子TNF-α和NF-κB以及NO和VEGFA系统组分的表达。他们还测量了血糖水平和体重。此外，还评估了补充l -精氨酸对相同参数的影响。STZ组和STZ + l -精氨酸组的体重低于对照组和l -精氨酸组。补充l -精氨酸可以降低糖尿病大鼠的血糖水平并增加胰岛素水平。糖尿病组胰腺β细胞出现退行性改变。胰岛素在STZ组表达较低，而在STZ + l -精氨酸组表达较高。在对照组和l -精氨酸组中，VEGFA均定位于胰岛、腺体上皮细胞、小管和内皮细胞。在STZ和STZ + l -精氨酸组中，VEGFA的定位模式相似。在对照组和l -精氨酸组的胰岛中检测到eNOS和VEGFR2/FLK1/KDR的表达。在STZ和STZ + l -精氨酸组中表达量较低。然而，VEGFR2/FLK1/KDR信号强度在STZ + l -精氨酸组强于STZ组。STZ组iNOS、活性caspase-3、TNF-α、NF-κB表达水平显著高于对照组。综上所述，尽管l -精氨酸治疗逆转了stz诱导的高血糖和胰岛素缺乏，但对iNOS、活性caspase-3、TNF-α和NF-κB的表达水平没有显著影响。此外，l -精氨酸通过增加VEGFR2/FLK1/KDR表达对胰岛血管维持至关重要。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.