Evaluation of the potential protective effect of hydrogen-rich saline against brain injury in experimental head trauma model in rats.

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-12-01 Epub Date: 2025-08-05 DOI:10.1016/j.tice.2025.103076

Murat Kayabaş, Mustafa Makav, Serdar Yi̇ği̇t, Levent Aras, Levent Şahi̇n, Menekşe Bulut, Duried Alwazeer

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引用次数: 0

Abstract

‎Head trauma is one of the main reasons for morbidity and mortality in motor vehicle and work accidents. This study aimed to evaluate the effect of hydrogen on head trauma injury. The study groups were divided into four groups, with six rats in each group as follows: Control or no treatment (group C), Head Trauma Injury (group HI), Hydrogen-rich Saline (group H2), and Head Trauma Injury + hydrogen-rich saline (group HI+H2). Blood and brain tissues, serum, interleukin (IL-6), and tumor necrosis factor (TNF-α) parameters were analyzed. Histopathologically, hematoxylin-eosin and TUNEL staining were performed on the tissue samples. A significant increase in TNF-α and IL-6 levels in the HI group that decreased when hydrogen-rich saline was applied, i.e., the HI+H2 group. Histopathologically, a decrease in degenerative cells was observed in the treatment group (i.e., HI+H2). TUNEL staining also showed a decrease in the number of stained cells in the HI+H2 group compared to the HI group. It is thought that hydrogen-rich saline treatment may be suggested to alleviate the damage caused by head trauma injury.

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富氢盐水对实验性颅脑损伤模型大鼠脑损伤潜在保护作用的评价。

头部创伤是机动车和工作事故中发病率和死亡率的主要原因之一。本研究旨在评价氢对头部创伤损伤的影响。将实验组分为4组，每组6只，分别为：对照组或未治疗组（C组）、头部外伤组（HI组）、富氢盐水组（H2组）、头部外伤+富氢盐水组（HI+H2组）。分析血、脑组织、血清、白细胞介素（IL-6）、肿瘤坏死因子（TNF-α）等指标。组织病理学上，对组织样本进行苏木精-伊红染色和TUNEL染色。HI组（即HI+H2组）TNF-α和IL-6水平显著升高，富氢盐水组（即HI+H2组）TNF-α和IL-6水平降低。组织病理学上，观察到治疗组（即HI+H2）退行性细胞减少。TUNEL染色也显示，与HI组相比，HI+H2组染色细胞数量减少。认为富氢盐水治疗可减轻颅脑外伤的损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.