Tissue & cell最新文献

{"title":"Arbutin improves post-myocardial infarction cardiac dysfunction by inhibiting cardiac fibroblast activation","authors":"Yun Liu ,&nbsp;Xuemei Liu ,&nbsp;Xuan Zhang ,&nbsp;Hongwei Yue ,&nbsp;Chang Pan","doi":"10.1016/j.tice.2025.102986","DOIUrl":"10.1016/j.tice.2025.102986","url":null,"abstract":"<div><h3>Background</h3><div>Arbutin exhibits multiple effects, including anti-inflammatory, antioxidant, and scavenging of free radicals, yet its research within the cardiovascular system remains limited.</div></div><div><h3>Purpose</h3><div>This study aims to investigate the effects of arbutin on myocardial infarction induced by left anterior descending (LAD) ligation in mice.</div></div><div><h3>Methods</h3><div>The possible mechanism of arbutin's effect on myocardial infarction was predicted through network pharmacology studies. To evaluate the impact of arbutin on cardiac function in myocardial infarction, TTC staining of the heart was performed, and echocardiography was conducted on mice at different time points. Furthermore, Western blot analysis was utilized not only to detect αSMA, Collagen I, and Collagen III, aiming to investigate the impact of arbutin on myocardial fibrosis but also to detect the influence of arbutin on the phosphorylation level of ERK1/2, thereby elucidating its potential mechanism of action.</div></div><div><h3>Results</h3><div>Network pharmacology data suggest that arbutin may exert a beneficial effect on myocardial infarction by modulating the ERK1/2 signaling pathway. In vivo experimental results indicate that after myocardial infarction, arbutin can alleviate myocardial fibrosis and cardiac hypertrophy, significantly improving cardiac function. Further in vitro experiments confirm that arbutin markedly reduces fibrosis-related indicators, and this process is partially achieved through the regulation of ERK1/2 phosphorylation.</div></div><div><h3>Conclusion</h3><div>In conclusion, this study reveals that arbutin exerts a significant protective effect by acting on the ERK1/2 signaling pathway, effectively inhibiting myocardial fibrosis, and subsequently attenuating myocardial infarction induced by LAD ligation.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102986"},"PeriodicalIF":2.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promotion of skin regeneration in diabetic rats by collagen-based hydrogel incorporated with basic fibroblast growth factor: A histological, molecular, and tensiometrical study","authors":"Melody Omraninava ,&nbsp;Rafat Rezapour-Nasrabad ,&nbsp;Mojgan Hosseini ,&nbsp;Mohammad Armin Kasiri ,&nbsp;Shadman Shahzamani ,&nbsp;Maryam Bahrami ,&nbsp;Zahra Sadrzadeh-aghajani ,&nbsp;Mohamad Sedigh Mirzaie","doi":"10.1016/j.tice.2025.102983","DOIUrl":"10.1016/j.tice.2025.102983","url":null,"abstract":"<div><div>Diabetic wounds represent a major and costly challenge for diabetic patients, leading to significant morbidity and healthcare expenses. Consequently, extensive research has been dedicated to identifying effective treatments to enhance wound healing. Among these, biological hydrogels have emerged as promising candidates due to their superior properties over traditional materials. This study aimed to assess the efficacy of a bioactive and biodegradable collagen-based hydrogel derived from human amniotic membrane (CHA) combined with basic fibroblast growth factor (bFGF) in promoting wound healing in diabetic rats. A total of thirty diabetic rats were randomly divided into three groups (n = 10): control, CHA, and CHA incorporated with bFGF (CHA+bFGF). Wound evaluations were conducted on days 7 and 21. The findings revealed notable improvements in wound closure, fibroblast and blood vessel counts, collagen density, tensiometrical parameters, and the levels of VEGF in the treatment groups compared to the control group, with the most significant effects observed in the CHA+bFGF group. Moreover, the CHA+bFGF group demonstrated a greater reduction in inflammatory cells infiltration, along with lower concentration of TNF-α and IL-1β cytokines, compared to the other groups. In conclusion, the combination of CHA with bFGF proved to be highly effective in enhancing the healing process of diabetic wounds.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102983"},"PeriodicalIF":2.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of misoprostol in the prevention of amikacin-induced neuronal damage","authors":"Sebile Azırak ,&nbsp;İlkay Armağan","doi":"10.1016/j.tice.2025.102981","DOIUrl":"10.1016/j.tice.2025.102981","url":null,"abstract":"<div><div>Amikacin (AK) is an aminoglycoside widely used in the treatment of Gram-negative infections which are life-threatening. In the etiology of toxicity, AK has been shown to cause tissue damage through oxidative stress and apoptosis as a consequence of the reactive oxygen species (ROS) production. Misoprostol (MP) is a prostaglangin E1 (PGE1) analogue with antioxidant, antiapoptotic, cytoprotective properties used to prevent gastrointestinal disorders induced by nonsteroidal anti-inflammatory drug. This study aims to investigate the neuroprotective effects of MP on brain cells against the neurotoxicity of AK by gene expression and histopathological analyses. Twenty-four male Spraque-Dawley rats were randomly separated into four groups (group 1, control; group 2, AK; group 3, MP; group 4, AK + MP). According to our findings, AK treatment significantly increased brain weight, brain weight/body weight ratios, and CYP2B1 mRNA gene expression. This was accompanied by histopathological changes such as eosinophilic neurons, pyknotic nuclei, vacuolated neuropil, congestion and inflammation in blood vessels. On the other hand, while significant improvements were observed in brain weight and brain weight/body weight ratios in the AK + MP group, there was a significant decrease in CYP2B1 mRNA gene expression levels and histopathological changes. According to our results, MP may serve as a potential therapeutic candidate to reduce the severity of oxidative damage induced by AK in brain tissue.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102981"},"PeriodicalIF":2.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleolin alleviates endotoxemia-induced myocardial dysfunction via inhibiting Drp1-mediated mitochondrial fission","authors":"Ludong Yuan ,&nbsp;Yuting Tang ,&nbsp;Leijing Yin ,&nbsp;Xiaofang Lin ,&nbsp;Pengfei Liang ,&nbsp;Bimei Jiang","doi":"10.1016/j.tice.2025.102964","DOIUrl":"10.1016/j.tice.2025.102964","url":null,"abstract":"<div><h3>Background</h3><div>Our previous study found that nucleolin expression exerted anti-cardiac injury effects by promoting mitochondrial biogenesis; however, it could not explain the increase in mitochondrial fragmentation during myocardial injury. Mitochondrial fragmentation is associated with mitochondrial fission, but it is unknown whether nucleolin regulates mitochondrial fission. Therefore, this study aims to investigate the mechanism by which nucleolin regulates mitochondrial fission in endotoxemia-induced myocardial dysfunction.</div></div><div><h3>Methods</h3><div>Nucleolin myocardial-specific knockout mice were used to construct an endotoxemia-induced myocardial dysfunction model. Mitochondrial membrane potential (MMP), ATP production, Mitotracker Red, Transmission Electron Microscope were measured to assess mitochondrial function. Mitochondria were isolated to observe Drp1 translocation to mitochondria. The expression of pGSK-3β-Tyr216, GSK-3β, pDrp1-Ser637, nucleolin and dynamin-related protein 1 (DNM1L, Drp1) were detected using qRT-PCR and western blot.</div></div><div><h3>Results</h3><div>Following cecum ligation and puncture (CLP) model, cardiac function was impaired, myocardial mitochondrial function declined, mitochondrial morphology became disorganized and fragmented, nucleolin and Drp1 expression was elevated. Myocardial injury and mitochondrial dysfunction were further exacerbated after nucleolin myocardium-specific knockout. Meanwhile, after cellular-level nucleolin interference, it further led to LPS and TNF-α-induced mitochondrial dysfunction and cardiomyocyte damage. Mechanically, nucleolin interference inhibited Drp1 phosphorylation at Ser637 and promoted Drp1 translocation to mitochondria. Myocardial injury caused by nucleolin knockdown was alleviated by the use of P110, an inhibitor of Drp1 mitochondrial translocation.</div></div><div><h3>Conclusion</h3><div>Endotoxemia-induced myocardial dysfunction is accompanied by increased mitochondrial fragmentation. Nucleolin alleviates endotoxemia-induced myocardial dysfunction by enhancing Drp1 phosphorylation at Ser637, inhibiting Drp1 translocation to the mitochondria and mitochondrial fission.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102964"},"PeriodicalIF":2.7,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periostin enhances paracrine effect of adipose-derived stem cells on chondrocyte proliferation, adhesion and migration","authors":"Ran Lu,&nbsp;Yong Hou,&nbsp;Xiaogao Wang,&nbsp;Zeyu Guan,&nbsp;Zhonglin Nie,&nbsp;Chaowen Yu,&nbsp;Yong Gao","doi":"10.1016/j.tice.2025.102979","DOIUrl":"10.1016/j.tice.2025.102979","url":null,"abstract":"<div><h3>Background</h3><div>Articular cartilage regeneration after injury remains a difficult clinical problem. Studies have revealed that adipose-derived stem cells (ADSCs) can promote chondrocyte regeneration. This study explored the role of Periostin, a multifunctional extracellular matrix protein, in enhancing the paracrine-mediated effects of ADSCs to promote chondrocyte regeneration.</div></div><div><h3>Material and methods</h3><div>ADSCs were isolated from murine adipose tissue and characterized. Periostin was overexpressed via a lentiviral vector in ADSCs (P-ADSCs). Chondrocytes were isolated and cocultured with P-ADSCs, followed by assessing of cell viability, apoptosis, cell migration and adhesion. In addition, periostin concentrations in the co-culture medium were measured over time by ELISA and detailed densitometric analyses of signaling proteins were performed.</div></div><div><h3>Results</h3><div>Compared with control ADSCs, P-ADSCs significantly enhanced chondrocyte proliferation, reduced apoptosis, and promoted migration and adhesion. ELISA quantification revealed that chondrocytes co-cultured with P-ADSCs showed a significant decrease in pro-inflammatory cytokines of IL-1β, IL-6, IL-18 with less than 0.6 fold-changes, and a significant increase in growth factors of TGF-β, b-FGF, PDGF with more than 4 fold-changes. Moreover, qPCR and immunoblotting demonstrated upregulation of key chondrocyte markers collagen II and Sox9, and activation of FAK/PI3K/Akt/ERK signals.</div></div><div><h3>Conclusions</h3><div>Periostin overexpression in ADSCs enhances their paracrine effect, promoting chondrocyte proliferation, adhesion, migration, and favorable phenotypic marker expression while modulating cytokine secretion. These findings provide a potential strategy for enhancing ADSC-mediated cartilage repair.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102979"},"PeriodicalIF":2.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resolvin D5: A lipid mediator with a therapeutic effect on hepatic steatosis through SIRT6/autophagy","authors":"Do Su Lim ,&nbsp;Sung Ho Ahn ,&nbsp;Hyeon Ji Gwon ,&nbsp;Wonjun Cho ,&nbsp;A.M. Abd El-Aty ,&nbsp;Haci Ahmet Aydemir ,&nbsp;Naveen Sharma ,&nbsp;Soon Auck Hong ,&nbsp;Tae Woo Jung ,&nbsp;Ji Hoon Jeong","doi":"10.1016/j.tice.2025.102980","DOIUrl":"10.1016/j.tice.2025.102980","url":null,"abstract":"<div><div>Resolvin D5 (RD5), a lipid mediator derived from DHA <em>via</em> 5-lipoxygenase signaling, has been shown to resolve inflammation in various disease models. This study aimed to investigate the role of RD5 in the development of hepatic steatosis in individuals with obesity and explore the detailed mechanisms involved. Protein expression was evaluated <em>via</em> Western blot analysis, whereas hepatic lipid deposition was examined <em>via</em> Oil Red O staining and triglyceride quantification. Autophagosomes were detected <em>via</em> MDC staining. Our findings indicated that RD5 treatment normalized lipogenic lipid accumulation, fatty acid uptake, oxidation, apoptosis, and endoplasmic reticulum (ER) stress in palmitate-treated primary hepatocytes. As a cytoprotective signaling pathway, RD5 treatment increased the expression of SIRT6 and autophagy markers, such as those involved in LC3 conversion and p62 degradation. The beneficial effects of RD5 on hepatic lipid metabolism, apoptosis, and ER stress were negated by SIRT6 small interfering RNA or 3-methyladenine, an inhibitor of autophagy. Furthermore, RD5 administration decreased hepatic steatosis, apoptosis, and ER stress in the livers of high-fat diet (HFD)-fed mice. In line with the <em>in vitro</em> results, RD5 treatment elevated SIRT6 and autophagy levels in the livers of HFD-fed mice. These novel findings suggest that RD5 improves hepatic lipid metabolism, apoptosis and ER stress through SIRT6/autophagy signaling, thereby attenuating hepatic steatosis. RD5 may have therapeutic potential for treating nonalcoholic fatty liver disease with minimal side effects.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102980"},"PeriodicalIF":2.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autophagic impairment in endometrial polyps: A potential biomarker and therapeutic target","authors":"Burak Sezgi̇n ,&nbsp;Tuba Edgünlü ,&nbsp;Özgür ilhan Çeli̇k ,&nbsp;Nazlı Can ,&nbsp;Ayşegül Demirtaş Bi̇lgi̇ç","doi":"10.1016/j.tice.2025.102978","DOIUrl":"10.1016/j.tice.2025.102978","url":null,"abstract":"<div><div>Endometrial polyps are a common gynecological condition associated with abnormal uterine bleeding, infertility, and potential malignancies. This study investigated the expression of key autophagy proteins LC3A/B, p62, and Beclin-1 in endometrial polyps. Twenty patients with endometrial polyps and ten healthy controls were enrolled in a prospective randomized controlled trial. Tissue samples were collected via operative hysteroscopy. ELISA and immunohistochemistry were employed to assess the expression of LC3A/B, p62, and Beclin-1. While ELISA results did not reveal significant differences between the two groups, immunohistochemical analysis demonstrated significantly lower levels of LC3A/B, p62, and Beclin-1 in endometrial polyps compared to healthy endometrial tissue (p = 0.041, p = 0.012, and p = 0.003, respectively). These findings suggest that impaired autophagy, as evidenced by reduced levels of these key autophagy proteins, may contribute to the pathogenesis of endometrial polyps. Further research is needed to elucidate the precise mechanisms underlying this association and to explore potential therapeutic implications.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102978"},"PeriodicalIF":2.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal control of the asymmetric uterus of the bat Molossus molossus during pregnancy and lactation: Emphasis on progesterone and estradiol","authors":"Juliana F. Ferraz ,&nbsp;Rodrigo S. de Oliveira ,&nbsp;Cornélio S. Santiago ,&nbsp;Emília M. Soares ,&nbsp;Rejane M. Góes ,&nbsp;Eliana Morielle-Versute ,&nbsp;Sebastião R. Taboga ,&nbsp;Mateus R. Beguelini","doi":"10.1016/j.tice.2025.102974","DOIUrl":"10.1016/j.tice.2025.102974","url":null,"abstract":"<div><div><em>Molossus molossus</em> is an important species of Neotropical insectivorous bat of the family Molossidae. Despite its wide distribution, representativeness, and ecological importance, there are no detailed studies regarding the hormonal control of the female reproductive organs of this species, which presents accentuated morphofunctional asymmetry, with dextro-dominance. Thus, the present study aimed to evaluate the variations in hormone serum levels and in uterine hormonal control of <em>M. molossus</em> during its different reproductive phases, with an emphasis on estradiol and progesterone. Twenty sexually adult females were divided into four sample groups according to the reproductive status — non-reproductive, early pregnancy, late pregnancy and lactating — and subjected to histological, hormonal, and immunohistochemical analysis. The results showed that the uterine dextro-dominance asymmetry of <em>M. molossus</em> is strongly regulated and maintained by the interaction/cross-talk between estradiol and progesterone serum levels with their respective uterine receptors. The right uterine horn presents significantly higher estrogen receptor α and progesterone receptor expression and also greater proliferative activity in the endometrium than the left horn, which favors embryo implantation and development. Significant increases in the estradiol and progesterone serum levels are needed to regulate early pregnancy. The rapid formation of the diffuse portion of the placenta is essential to sustain the high demand for progesterone at the beginning of pregnancy, while the principal portion is not yet formed. Estrogen receptor α and progesterone receptor expression appear to be synchronized to coordinate most of the processes that occur within the uterus of <em>M. molossus</em> throughout the reproductive cycle.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102974"},"PeriodicalIF":2.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hedera helix folium extract attenuates methotrexate-induced hepatotoxicity by modulating oxidative stress and inflammatory mediators","authors":"Esra Deniz ,&nbsp;Atilla Topcu ,&nbsp;Aykut Ozturk ,&nbsp;Seda Duman Ozturk ,&nbsp;Kerimali Akyildiz","doi":"10.1016/j.tice.2025.102967","DOIUrl":"10.1016/j.tice.2025.102967","url":null,"abstract":"<div><div>Methotrexate (MTX)-induced hepatotoxicity is linked to oxidative damage and inflammatory processes. <em>Hedera helix folium</em> (HHF) extract protects cells against oxidative damage. We investigated the role of HHF extract in tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10)-associated inflammation and oxidative stress in the pathology of MTX-associated liver injury in rats. Forty male rats were divided into one of five equal groups: Control, HHF, MTX, H₁₀₀+MTX and H₂₀₀+MTX. HHF extract was administered via the oral route at 100 mg/kg or 200 mg/kg once daily for seven days, while MTX was administered as a single dose of 20 mg/kg intraperitoneally. Intracardiac blood samples and liver tissue samples were collected at the conclusion of the experiment. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels increased due to MTX. Increased ALT levels were significantly reduced by low-dose HHF and increased AST levels were significantly reduced by high-dose HHF administration. The application of MTX significantly increased malondialdehyde (MDA) and TNF-α levels, while significantly reducing those of glutathione (GSH) and IL-10. High-dose HHF also significantly lowered MDA and TNF-α levels, while significantly increasing those of GSH and IL-10<em>.</em> Histopathological damage findings observed due to MTX were significantly attenuated with high-dose HHF. In addition, the increased caspase-3, p53, and Bcl2 levels caused by MTX decreased with high-dose HHF administration. HHF extract can alleviate liver damage induced by MTX. This extract, which has the ability to reduce damage due to oxidative stress and inflammation, may represent an alternative approach to preventing MTX-induced liver damage.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102967"},"PeriodicalIF":2.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dorso-ventral skin of flowered racer snake (coluber flourulentus, perreti schatti, 1988): Histological, Keratin immunohistochemical, and ultrastructural characterization","authors":"Ramadan Kandyel ,&nbsp;Saeed Alasmari ,&nbsp;Salem Alharethi ,&nbsp;Bader Albogami ,&nbsp;Abdulrhman Almadiy ,&nbsp;Doaa Gewily ,&nbsp;Fatma A. Madkour ,&nbsp;Mohamed Abumandour","doi":"10.1016/j.tice.2025.102966","DOIUrl":"10.1016/j.tice.2025.102966","url":null,"abstract":"<div><div>Our study, using SEM and histology, aims to characterize the dorsal and ventral skin of <em>Coluber flourulentus</em>, providing new insights into their Keratin immunohistochemical features. The SEM findings revealed overlapping smooth scales on both surfaces, with minor scale morphology variations; the dorsal scales were finger-shaped with a pointed apex, while the ventral scales were flattened with tooth-like edges. Scales on both surfaces reveal two large, spherical-shaped lenticular sensory organs at their apex, dome-shaped receptors/sensilla on the outer surface, and dome-shaped sensilla with dendrites, a transverse cleft, and various pits and pores. Histologically, the micro-ornamentations on the inner-scale surfaces of both surfaces had similar structures with smooth, spiny scales and two layers. The epidermis layer had several layers, including the basal stratum germinativum, α-keratin, the Mesos layer, the β-layer, and the covered Oberhautchen layer. Skin pigmentation varied between both surfaces: the dorsal dermal layer contains melanophores and iridophores, and the ventral dermal layer contains only dark black melanophores under the epidermal layer. Histochemical collagen fiber observations revealed blue-colored fibers in the dermis of dorsal and ventral surfaces, sparse underneath the basement membrane, and visible around pigment cells and muscle bundles. A dark brown reaction was observed in the epidermal cells of both dorsal and ventral surfaces, with high cytokeratin immunolabeling on the ventral surface and moderate cytokeratin immunoreaction on the dorsal surface. In conclusion, scales were crucial for controlling movements and responding to vibrational stimuli from object movements, including those of potential prey or predators.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102966"},"PeriodicalIF":2.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}