Tissue & cell最新文献_第2页

Zinc finger protein 383 suppresses tumor growth in NSCLC by upregulating DUSP16 expression to inactivate ERK signaling. 锌指蛋白383通过上调DUSP16表达使ERK信号失活来抑制非小细胞肺癌的肿瘤生长。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-10-01 Epub Date: 2026-05-26 DOI: 10.1016/j.tice.2026.103640

Jingjing Wu, Shanshan Huang, Xiangna Chen, Haifeng Yu

{"title":"Zinc finger protein 383 suppresses tumor growth in NSCLC by upregulating DUSP16 expression to inactivate ERK signaling.","authors":"Jingjing Wu, Shanshan Huang, Xiangna Chen, Haifeng Yu","doi":"10.1016/j.tice.2026.103640","DOIUrl":"10.1016/j.tice.2026.103640","url":null,"abstract":"Purpose: Despite its known function as a KRAB-domain zinc finger protein and MAPK pathway suppressor, the specific role of Zinc finger protein 383 (ZNF383) in non-small cell lung cancer (NSCLC) remains elusive. To address this, we assessed ZNF383 expression in NSCLC and examined its effects on tumor cell proliferation.Methods: We performed RT-qPCR, western blotting, and immunohistochemistry to detect ZNF383 expression in NSCLC cells and tissues. Survival outcomes were assessed by the Kaplan-Meier method. The functional role of ZNF383 was elucidated through a series of in vitro and in vivo experiments. To investigate its transcriptional regulatory mechanism, we conducted dual-luciferase reporter assays and confirmed specific promoter binding via ChIP-qPCR.Results: Low ZNF383 expression in NSCLC patients was linked to aggressive clinicopathology (poor differentiation, advanced stage, metastasis) and poor survival. ZNF383 acted as a tumor suppressor: its overexpression inhibited cell proliferation, colony formation, cell cycle progression, and tumor growth, whereas its knockdown promoted these processes. Mechanistically, ZNF383 (via its KRAB domain) upregulate DUSP16, leading to ERK inactivation, p27 accumulation, and G1 arrest.Conclusion: ZNF383 upregulate DUSP16 expression, consequently inactivating ERK signaling to inhibit NSCLC progression. Targeting the ZNF383/DUSP16/ERK axis presents a novel strategy for NSCLC therapy.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"102 ","pages":"103640"},"PeriodicalIF":3.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148101890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanisms of estrogen-mediated GPER/YAP pathway in benign hyperplastic prostate. 雌激素介导的GPER/YAP通路在良性前列腺增生中的作用机制。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-10-01 Epub Date: 2026-05-25 DOI: 10.1016/j.tice.2026.103641

Yuanhang Zhao, Yuanjie Niu

{"title":"The mechanisms of estrogen-mediated GPER/YAP pathway in benign hyperplastic prostate.","authors":"Yuanhang Zhao, Yuanjie Niu","doi":"10.1016/j.tice.2026.103641","DOIUrl":"10.1016/j.tice.2026.103641","url":null,"abstract":"Benign prostatic hyperplasia (BPH) is a common age-related condition characterized by prostate enlargement, which is influenced by hormonal imbalances and altered signaling pathways. In this study, we investigated the effects of different doses of treatment on prostate tissue in a rat model of BPH, focusing on the G protein‑coupled estrogen receptor (GPER), the downstream signaling molecule Yes-associated protein (YAP), and its phosphorylated form (p-YAP). We measured hormone levels, protein expression, and histopathological changes to evaluate the impact of treatment on prostate growth. Serum estrogen and testosterone levels were assessed using enzyme‑linked immunosorbent assay (ELISA), while GPER, YAP, and p-YAP protein expression were analyzed by Western blot (WB). Immunofluorescence assays for Ki-67 and Caspase-3 were also performed. Our results showed that 17β-estradiol (E2) 50 μg/kg and E2 100 μg/kg treatment significantly increased GPER and p-YAP protein expression, with corresponding increases in prostate tissue proliferation and reduced apoptosis, as indicated by Ki-67 and Caspase-3 expression. In contrast, the inhibitor treatment significantly decreased GPER and p-YAP expression, promoting apoptosis and reducing prostate enlargement. Hormonal assays revealed that estrogen levels were elevated and testosterone levels were reduced in the E2 50 μg/kg and E2 100 μg/kg groups, whereas inhibitor treatment partially reversed these changes in circulating and intraprostatic sex hormone levels. These findings suggest that the GPER/YAP signaling pathway plays a key role in BPH progression and that targeting this pathway may offer a novel therapeutic approach for managing BPH.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"102 ","pages":"103641"},"PeriodicalIF":3.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148101857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Paulownia tomentosa leaf extract mitigates lambda-cyhalothrin-induced thyroid dysfunction via modulation of Nrf2/HO-1, NF-κB, and Bax/Bcl-2 signaling pathways in mice" [Tissue Cell 101 (2026) 103422]. “泡桐叶提取物通过调节Nrf2/HO-1、NF-κB和Bax/Bcl-2信号通路减轻lambda- cyhalthrin诱导的小鼠甲状腺功能障碍”[组织细胞101(2026)103422]。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-10-01 Epub Date: 2026-03-27 DOI: 10.1016/j.tice.2026.103488

Samah A El-Hashash, Ethar E Elkholy, Asmaa M El-Gawish, Mustafa Shukry, Ibrahim Albokhadaim, Sameer Alhojaily, Khalid M Al Khodair

引用次数: 0

Corrigendum to "Oligosaccharides ameliorate insulin resistance and hepatic metabolism by promoting the leptin/POMC axis to accelerate short stature growth and development" [Tissue Cell 99 (2026) 103277]. “低聚糖通过促进瘦素/POMC轴加速矮小身材的生长和发育，改善胰岛素抵抗和肝脏代谢”[Tissue Cell 99(2026) 103277]的更正。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-10-01 Epub Date: 2026-05-16 DOI: 10.1016/j.tice.2026.103619

Minhong Xu, Jin Zhou, Shuyao Zhang, Hongli Wang, Yongtao Zhang, Yinan Liu, Chengkuan Zhao

引用次数: 0

Corrigendum to "M2 macrophage-derived migrasomes enhance bone regeneration by directing osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs)" [Tissue Cell 100 (2026) 103394]. “M2巨噬细胞衍生的迁移体通过引导骨髓间充质干细胞（BMSCs）的成骨分化来促进骨再生”[Tissue Cell 100(2026) 103394]的更正。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-10-01 Epub Date: 2026-04-01 DOI: 10.1016/j.tice.2026.103511

Mengci Wang, Siyu Zhang, Abudurexiti Kutibiding, Xiaodan Zhou, Xiaohan Tian, Sunqiang Yuan, Mengjiao Lv, Yi Yang, Shengbin Bai

引用次数: 0

Corrigendum to "Ultrastructure and histological features of the Pecten oculi of the African Swamphen (Porphyrio madagascariensis): with special focus on its SEM-EDX analysis and its migratory behaviour" [Tissue Cell 100 (2026) 103356]. “非洲沼泽羚（Porphyrio马达加利ensis）眼胶的显微结构和组织学特征：特别关注其SEM-EDX分析及其迁移行为”的勘误[组织细胞100(2026)103356]。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-10-01 Epub Date: 2026-03-28 DOI: 10.1016/j.tice.2026.103484

Ramadan Kandyel, Saeed Alasmari, Salem H Alharethi, Abdulrhman A Almadiy, Bader Albogami, Diaa Massoud, Mohamed Abumandour

引用次数: 0

Single-cell transcriptomic analysis for the identification and validation of key genes associated with metabolic cell death in pressure ulcers. 单细胞转录组学分析用于鉴定和验证与压疮代谢细胞死亡相关的关键基因。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-10-01 Epub Date: 2026-05-28 DOI: 10.1016/j.tice.2026.103643

Jianfeng Dong, Yijie Ning, Chuanlong Lu, Wen Wu, Kewei Zhang, Huo Chai, Huijia Dong, Jian Sun

{"title":"Single-cell transcriptomic analysis for the identification and validation of key genes associated with metabolic cell death in pressure ulcers.","authors":"Jianfeng Dong, Yijie Ning, Chuanlong Lu, Wen Wu, Kewei Zhang, Huo Chai, Huijia Dong, Jian Sun","doi":"10.1016/j.tice.2026.103643","DOIUrl":"10.1016/j.tice.2026.103643","url":null,"abstract":"Background: Pressure ulcer (PU) result from sustained pressure and friction that damage the skin and underlying subcutaneous tissues. Although ferroptosis-related proteins are differentially expressed between PUs and normal skin, the role of metabolic cell death (MCD)-related genes in PUs remains unclear. In this study, we aimed to identify MCD-related genes (MCD-RGs) associated with PUs using single-cell transcriptomic analysis and to explore their potential mechanisms.Methods: Differentially expressed genes were identified in key cell clusters from the PU single-cell dataset GSE137897 and intersected with MCD-RGs to obtain candidate genes. Key genes were screened through protein-protein interaction (PPI) network analysis, followed by regulatory network construction, drug prediction, enrichment analysis, pseudotime analysis, and validation using reverse transcription quantitative polymerase chain reaction (RT-qPCR).Results: Key cells included immune cell clusters 1 and 2 and melanocytes. A total of 78 candidate genes and eight key genes were identified. These genes were predicted to be regulated by 40 transcription factors (TFs), to be associated with relevant biological pathways, and to be linked to 58 potential compounds. JUN differed significantly across all key cells, whereas ACTB showed stable changes. RT-qPCR results showed that ACTB, IKBKB, and JUN were upregulated in PUs.Conclusion: The integration of eight key genes identified through single-cell transcriptomic analysis provides new insights into the molecular mechanisms of PU and may help identify potential therapeutic targets.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"102 ","pages":"103643"},"PeriodicalIF":3.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148139037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors released from the human placentae, including extracellular vesicles, reduce the migration of endometriotic cells: A potential therapeutic for endometriosis. 从人胎盘释放的因子，包括细胞外囊泡，减少子宫内膜异位症细胞的迁移：子宫内膜异位症的潜在治疗方法。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-09-29 DOI: 10.1016/j.tice.2026.103998

Lin Wang, Jinqiu Zhang, Angang Sun, Ye Shen, Qi Chen, Min Zhao

{"title":"Factors released from the human placentae, including extracellular vesicles, reduce the migration of endometriotic cells: A potential therapeutic for endometriosis.","authors":"Lin Wang, Jinqiu Zhang, Angang Sun, Ye Shen, Qi Chen, Min Zhao","doi":"10.1016/j.tice.2026.103998","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103998","url":null,"abstract":"Endometriosis is characterised by the growth of abnormal endometrial tissue outside the uterus and chronic inflammation. Increasing evidence suggests that placenta extracellular vesicles (EVs) inhibit the growth and migration of ovarian and cervical cancer cells. This study investigated whether placental EVs have similar inhibitory effects on endometriotic cells. Human placental tissues were collected from the first trimester and cultured. The conditioned media were collected, and placental EVs were harvested. Endometriotic cell line 12Z cells were cultured with placental explant-conditioned media or placental EVs for 24 and 48 h. Cell proliferation, viability, migration, and invasion were then measured. The C19MC miRNAs were measured in 12Z cells after treatment. The proliferation and viability of 12Z cells were significantly reduced at 24 or 48 h after treatment with conditioned media compared with controls. Extracellular vesicles isolated from conditioned media also reduced proliferation and viability in 12Z cells, and this finding was confirmed by the transfection of miRNA mimics. Additionally, the migration and invasion of 12Z cells were significantly reduced after treatment. The levels of four C19MC miRNAs increased significantly in 12Z cells that had been treated with conditioned medium. This study demonstrates that factors released from the human placenta significantly inhibit endometriotic cell proliferation, migration, and inflammation. Placental EVs may contribute to these effects. These effects appear to be mediated, at least in part, by C19MC miRNAs. Our findings suggest that factors released from the human placentae, including extracellular vesicles, may be a novel therapeutic approach for managing endometriosis.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"104 Pt 2","pages":"103998"},"PeriodicalIF":3.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dihydromyricetin/F127 enhances fibroblast and endothelial responses and accelerates healing in debrided deep partial thickness burn wounds. 二氢杨梅素/F127增强成纤维细胞和内皮细胞的反应，加速深层部分厚度烧伤创面的愈合。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-09-25 DOI: 10.1016/j.tice.2026.103993

Ziyi Wang, Weiqin Zhao, Shuang Liu, Xinmei He, Xianghe Pi, Huiju Hu, Ni Pan, Boyuan Zhao, Na Dou

{"title":"Dihydromyricetin/F127 enhances fibroblast and endothelial responses and accelerates healing in debrided deep partial thickness burn wounds.","authors":"Ziyi Wang, Weiqin Zhao, Shuang Liu, Xinmei He, Xianghe Pi, Huiju Hu, Ni Pan, Boyuan Zhao, Na Dou","doi":"10.1016/j.tice.2026.103993","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103993","url":null,"abstract":"Debrided burn wounds require coordinated fibroblast, endothelial, and inflammatory responses for effective tissue repair. Dihydromyricetin (DHM) exhibits antioxidant and anti-inflammatory activities, but its concentration-response profile remains unclear. This study evaluated DHM in L929 fibroblasts and HUVECs and assessed a topical DHM/F127 formulation in a debrided contact-burn wound model. In vitro, 25-50 μM DHM increased L929 CCK-8 metabolic activity and scratch closure, while concurrent DHM treatment attenuated H2O2 associated oxidative stress. In HUVECs, 50 μM DHM increased scratch closure, and 25-50 μM enhanced tube formation. For in vivo validation, a standardized deep partial-thickness contact burn model was established in BALB/c mice, followed by debridement at 24 h after injury. Ten mice per group were randomly assigned to day 7 and day 14 endpoint cohorts, with five mice per endpoint. Wounds were treated daily with F127 or DHM/F127 containing 0.5 mg/mL DHM. Compared with F127, DHM/F127 further accelerated wound closure and reduced inflammatory infiltration at both endpoints, decreased MPO expression on day 7, and increased CD34 expression on day 14. These findings characterize DHM-associated fibroblast and endothelial responses and demonstrate additional tissue-level repair effects of DHM/F127 relative to F127 in debrided burn wounds.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"104 Pt 2","pages":"103993"},"PeriodicalIF":3.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149082108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spermatophore formation in Todarodes pacificus (Cephalopoda: Ommastrephidae). 太平洋蟾蜍精子包囊的形成（头足纲：巨蜥科）。

IF 3.1 4区生物学

Tissue & cell Pub Date : 2026-09-23 DOI: 10.1016/j.tice.2026.103985

So Ryung Shin, Jung Jun Park, Jung Sick Lee

{"title":"Spermatophore formation in Todarodes pacificus (Cephalopoda: Ommastrephidae).","authors":"So Ryung Shin, Jung Jun Park, Jung Sick Lee","doi":"10.1016/j.tice.2026.103985","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103985","url":null,"abstract":"This study elucidated the ultrastructure of spermatophore formation in Todarodes pacificus. The spermatophore of T. pacificus is a slender, elongated, club-shaped structure composed of an outer tunic, middle tunic, inner tunic, sperm mass, cement body, ejaculatory apparatus, and cap thread. Spermatophore formation occurred sequentially in the vas deferens, spermatophoric gland, and spermatophoric sac. In the vas deferens, sperm were aggregated and organized to form the sperm mass, and distinct differences in sperm arrangement were observed between the anterior and posterior regions. In the spermatophoric gland, the major components of the spermatophore-including the tunics, cement body, and ejaculatory apparatus-were formed. The epithelial layer of the spermatophoric gland consisted of epithelial and secretory cells containing fibrous and granular secretory materials. These secretory cells were classified into five types (Types A-E) according to their histochemical characteristics, and each type was associated with the formation of specific spermatophore components. The formed spermatophores were subsequently stored in the spermatophoric sac. This study provides a detailed microanatomical description of spermatophore formation in T. pacificus and offers fundamental insights into cephalopod reproductive physiology.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"104 Pt 2","pages":"103985"},"PeriodicalIF":3.1,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0