Tissue & cell最新文献

{"title":"Ling-Gui-Zhu-Gan decoction inhibits cardiomyocyte pyroptosis via the NLRP3/Caspase-1 signaling pathway","authors":"Xiao-ni Zhao ,&nbsp;Hui-min Ding ,&nbsp;Yao-yao Ma ,&nbsp;Liang Wang ,&nbsp;Peng Zhou","doi":"10.1016/j.tice.2024.102588","DOIUrl":"10.1016/j.tice.2024.102588","url":null,"abstract":"<div><h3>Objective</h3><div>The objective of this study was to investigate the protective mechanism of Ling-Gui-Zhu-Gan decoction (LGZGD) against LPS-ATP-induced pyroptosis in H9c2 cells.</div></div><div><h3>Methods</h3><div>LPS and ATP were used to induce pyroptosis in the H9c2 cell, and the cells were divided into the control, model and LGZGD groups. LDH level was detected using a colorimetric assay. ELISA was used to detect the expressions of IL-1β. Flow cytometry was utilized to observe apoptosis, while Hoechst/PI staining was used to detect pyroptosis. Immunofluorescence was employed to observe the expression levels of NLRP3 in cardiomyocytes, and RT-PCR was used to detect NLRP3, Caspase-1, GSDMD, and ASC mRNA expression. The cells were separated into seven groups: control, model, LGZGD, MCC950, LGZGD+MCC950, Nigericin and LGZGD+Nigericin. The mRNA and protein expressions were determined by RT-PCR and Western blot.</div></div><div><h3>Results</h3><div>LPS (10 μg/mL) for 12 h and ATP (8 mM) for 2 h were used as modeling condition. LGZGD demonstrated a significant reduction in LDH, and IL-1β levels (<em>P</em>&lt;0.05, <em>P</em>&lt;0.01). LGZGD dramatically reduced apoptosis rate, inhibited pyroptosis, decreased the fluorescence expressions of NLRP3, and reduced the mRNA expressions of NLRP3, ASC, Caspase-1, and GSDMD (<em>P</em>&lt;0.01). Further mechanism studies showed that NLRP3, ASC, Caspase-1, and GSDMD decreased significantly when combined with NLRP3 inhibitor MCC950. Furthermore, LGZGD was able to effectively reverse the upregulation of protein and gene expression of Nigericin group (<em>P</em>&lt;0.01).</div></div><div><h3>Conclusion</h3><div>LGZGD inhibits LPS-ATP-induced pyroptosis in H9c2 cell via the NLRP3/Caspase-1 signaling pathway.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential protection of resveratrol-tempeh against nephrotoxic and hepatotoxic histological damage in mice induced by aluminum","authors":"Yulia Irnidayanti ,&nbsp;Salsabilla Audy Julieta Wisaksono ,&nbsp;Win Darmanto ,&nbsp;Mahesh Narayan ,&nbsp;Hemen Sarma","doi":"10.1016/j.tice.2024.102589","DOIUrl":"10.1016/j.tice.2024.102589","url":null,"abstract":"<div><div>Aluminum is a widely distributed metal that, while generally safe at low levels, can become toxic when accumulated in the body. Its exposure is daily through various sources, including food, water, and medications. High levels of aluminum have been shown to adversely affect the kidneys and liver, leading to significant organ damage. Resveratrol-tempeh is a safe protective agent against organ damage caused by aluminum. Here, we investigated the impact of resveratrol on liver and kidney toxicity and Al-induced levels of catalase and malondialdehyde. The mice group was the control group, Al-group, Al+REST5-group, and Al+REST10-group. Aluminum and resveratrol were administered intraperitoneally to mice for four weeks, but resveratrol was administered one hour after exposure to aluminum. Mice were killed by cervical dislocation; the liver and kidney were isolated for slide, and the level of an antioxidant enzyme of catalase and oxidant of malondialdehyde was measured. The results showed that administration of aluminum at a dose of 200 mg/kg body weight caused glomerular shrinkage and proximal tubule degeneration in the kidneys. In addition, it also caused liver tissue damage, with hepatocytes undergoing degeneration, sinusoids dilating, and decreased body weight in the mice. Administration of resveratrol-tempeh tended to decrease malondialdehyde levels and increase catalase activity, although the changes were not significant. It seems that resveratrol-tempeh can repair liver and kidney damage and restore them to normal conditions. Conclusion: Aluminum at 200 mg/kg is toxic to mice. Resveratrol-tempeh can be considered a potential candidate to protect kidney and liver damage caused by aluminum chloride toxicity.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dietary L-carnitine supplementation on blood parameters and duodenal alterations in laying hens at the end of production","authors":"Hasan Bahrampour,&nbsp;Saied Mohammadzadeh,&nbsp;Mosaieb Amiri","doi":"10.1016/j.tice.2024.102585","DOIUrl":"10.1016/j.tice.2024.102585","url":null,"abstract":"<div><div>L-carnitine is an important nutritional supplement in the poultry industry, contributing to improved growth, production, and overall health of the birds. However, by the end of the production cycle, the endogenous synthesis of L-carnitine (LC) is often insufficient. This study aimed to evaluate the effects of dietary L-carnitine supplementation on blood parameters and duodenal structure in Ross laying hens during the last production phase. A total of 40 Ross strain laying hens, aged 70 weeks, were selected. The control group was administered a basal diet, while the experimental groups received the same diet supplemented with 100, 250, or 500 mg of L-carnitine per kg of the basal diet. The experimental period lasted for 56 days. Serum concentrations of cholesterol and total protein were not significantly affected by L-carnitine supplementation; however, triglyceride concentration and LDL levels were notably reduced. Furthermore, L-carnitine supplementation enhanced the villus perimeter and increased the villus length/crypt depth ratio. Importantly, the supplementation of 250 mg/kg of L-carnitine had a positive impact on duodenum structure and led to decreased levels of AST and ALP. In conclusion, the incorporation of 250 mg/kg of L-carnitine into the diet of laying hens significantly improved duodenal structure, reduced lipid peroxidation, and demonstrated antioxidant effects.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin triggers the Wnt/β-catenin pathway and shields neurons from injury caused by intermittent hypoxia","authors":"Yao He ,&nbsp;Yan Zhao ,&nbsp;Ren-jun Lv ,&nbsp;Na Dong ,&nbsp;Xiao Wang ,&nbsp;Qin Yu ,&nbsp;Hong-mei Yue","doi":"10.1016/j.tice.2024.102587","DOIUrl":"10.1016/j.tice.2024.102587","url":null,"abstract":"<div><div>The objective of this study was to explore the molecular basis through which Curcumin (Cur) mitigates neuronal damage caused by obstructive sleep apnea (OSA). HT22 was used to simulate intermittent hypoxia (IH) injury and explore the effect of Cur on these cells. We evaluated the cell viability, cytotoxicity, apoptosis, proliferation, and Wnt/β-catenin (WβC) pathway. IWR-1 was used to block the pathway and investigate the protective mechanism of Cur. We constructed an in vivo model of IH to validate the results of the cellular experiments. IH accelerated apoptosis and cytotoxicity, suppressed proliferation, and decreased the activity of the WβC pathway. Cur can significantly improve cell viability, reduce apoptosis rate and cell toxicity, promote cell proliferation, and up-regulate the WβC. After blocking the WβC pathway, the proliferative effect of Cur was observably weakened. In vivo, IH caused hippocampal damage and inhibited WβC pathway activity in mice, which was ameliorated by Cur treatment. This implies that Cur could be a novel treatment option for neurological impairment brought on by OSA.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating the complexities of cell death: Insights into accidental and programmed cell death","authors":"Mohammad-Sadegh Lotfi,&nbsp;Fatemeh B. Rassouli","doi":"10.1016/j.tice.2024.102586","DOIUrl":"10.1016/j.tice.2024.102586","url":null,"abstract":"<div><div>Cell death is a critical biological phenomenon that can be categorized into accidental cell death (ACD) and programmed cell death (PCD), each exhibiting distinct signaling, mechanistic and morphological characteristics. This paper provides a comprehensive overview of seven types of ACD, including coagulative, liquefactive, caseous, fat, fibrinoid, gangrenous and secondary necrosis, discussing their pathological implications in conditions such as ischemia and inflammation. Additionally, we review eighteen forms of PCD, encompassing autophagy, apoptosis, necroptosis, pyroptosis, paraptosis, ferroptosis, anoikis, entosis, NETosis, eryptosis, parthanatos, mitoptosis, and newly recognized types such as methuosis, autosis, alkaliptosis, oxeiptosis, cuprotosis and erebosis. The implications of these cell death modalities for cellular processes, development, and disease—particularly in the context of neoplastic and neurodegenerative disorders—are also covered. Furthermore, we explore the crosstalk between various forms of PCD, emphasizing how apoptotic mechanisms can influence pathways like necroptosis and pyroptosis. Understanding this interplay is crucial for elucidating cellular responses to stress, as well as for its potential relevance in clinical applications and therapeutic strategies. Future research should focus on clarifying the molecular mechanisms that govern different forms of PCD and their interactions.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol enhances sensitivity of renal cell carcinoma to tivozanib: An in-vitro study","authors":"Diana Taheri ,&nbsp;Helia Azodian Ghajar ,&nbsp;Akram Mirzaei ,&nbsp;Rahil Mashhadi ,&nbsp;Seyedeh Negin Hashemi Dougaheh ,&nbsp;Razman Arabzadeh Bahri ,&nbsp;Mahdi Khoshchehreh ,&nbsp;Ali Tavoosian ,&nbsp;Seyed Mohammad Kazem Aghamir","doi":"10.1016/j.tice.2024.102584","DOIUrl":"10.1016/j.tice.2024.102584","url":null,"abstract":"<div><h3>Background</h3><div>Since tivozanib has many side effects in the treatment of kidney cancer, we decided to use resveratrol as a bioactive molecule with anticancer and antioxidant properties to make tivozanib more effective and also reduce its side effects in kidney cancer cell line.</div></div><div><h3>Method</h3><div>In this in vitro study, we evaluated the effect of tivozanib, resveratrol and tivozanib- resveratrol combination therapy in ACHN cell line as representatives of human kidney cancer. The assessment includes Hoechst dye staining, scratch-wound assay, 3D spheroid, 2D colony formation assay, flow cytometric analysis of apoptosis and DNA cell cycle, real-time PCR (<em>BAX/BCL2</em>, <em>E-cadherin</em>, <em>Snail</em>, <em>HIF1α</em>, <em>VEGFC</em> and <em>KLK3</em> genes).</div></div><div><h3>Result</h3><div>To determine IC50 levels, ACHN cells was exposed to different concentration of tivozanib and resveratrol. Our data indicated that IC50 values for tivozanib (0.5 μM) and resveratrol (30 μM) with MTT in a dose and time-dependent manner. Due to the efficacy of resveratrol in combination with tivozanib, we used 20 μM resveratrol, and 0.25 μM tivozanib instead of 30 μM and 0.25 μM respectively. This data was approved by flow cytometry for ACHN cell line with 38.39, 14.74 and 66.06 percent apoptosis and 8.25, 5.12 and 15.6 percent subG1 for tivozanib, resveratrol and tivozanib-resveratrol combination respectively which was as a consequence of cell cycle arrest at G1/S phase. The treatment also reduced cells’ migration, fragmented nuclei, 3D spheroid and colony formation potentials in analyses. Evaluation of gene expression presented that the effect of the tivozanib and resveratrol combination in ACHN cell lines is completely different during the evaluation of apoptosis genes, <em>BAX, P53</em> genes and <em>E-Cadherin</em> had significantly increased expression compared to single treatment groups (P &lt; 0.01). Meanwhile, a significant decrease was observed in the expression of <em>VEGFC</em> and <em>HIF1α</em> genes in the combination group compared to the monotherapy groups (P &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>Considering that resveratrol can increase the apoptosis of cancer cells alone and in combination with tivozanib and prevent the proliferation of cancer cells and also reduce the side effects of tivozanib, we suggest that resveratrol as a potential bioactive molecule can be used in treatment of kidney cancer should be used in combination with tivozanib.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of tea tree oil and cefepime treatments on morphological, genetic, histopathological, immunohistochemistry, and biochemical assessments in liver and kidney of Escherichia coli infected rats","authors":"Abdallah Tageldein Mansour ,&nbsp;Amer Al Ali ,&nbsp;Taghreed N. Almanaa ,&nbsp;Dalia E. Altohamy ,&nbsp;Rasha M.M. Ezz-Eldin ,&nbsp;Mohammed S. Sobh ,&nbsp;Aaser M. Abdelazim ,&nbsp;Hanim S. Heikal ,&nbsp;Heba H. Mahboub ,&nbsp;Mohamed Aref","doi":"10.1016/j.tice.2024.102581","DOIUrl":"10.1016/j.tice.2024.102581","url":null,"abstract":"<div><div>The current study evaluated the influence of the treatment with tea tree oil and cefepime on morpho- genetic, histo-immunohistochemical, and biochemical assessments in rats experimentally challenged with <em>Escherichia coli</em> ATCC 4157™. Thirty adult male rats were divided into control, <em>E. coli</em> infected positive group (1×108CFU/I/P/once), <em>E. coli</em> with cefepime-supplemented group (45 mg/kg bw/I/M/day), <em>E. coli</em> with tea tree oil treated group (1.5 ml/per os/day), and the <em>E. coli-</em>challenged group that received a combination of tea tree oil and cefepime. <em>E. coli</em> infection induced morphological changes in color and texture of both liver and kidney. The transcription levels of the PHLPP2 and Nrf2 genes were noticeably lowered in all treated groups related to the <em>E. coli</em> group. Regarding the TLR4 expression, it was clearly up-regulated in the <em>E. coli</em> group in comparison to other groups, while CD14 gene decreased clearly in all treated groups compared to the positive group. The findings revealed that RBC, HGB, and PCV were clearly higher in the positive group compared to all treated groups. AST, ALT, and ALP, total bilirubin and its fractions, urea, and creatinine maximized in the positive group and decreased by the treatment, especially in the E+CF+oil treated group. Regarding the redox balance, MDA levels of MDA were notably reduced in the E+CF+oil treated group compared to the positive and the other treated groups. GSH, SOD, and GPX were significantly induced in the <em>E. coil</em>-treated group and decreased significantly with treatment. Overall, cefepime is highly safe especially when dually supplied with tea tree oil for mitigating <em>E. coli</em> adverse impact<em>.</em></div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and role of methyltransferase 3 in oral malignant transformation","authors":"Tianyi Zhang ,&nbsp;Haofeng Xiong ,&nbsp;Liujun Zeng ,&nbsp;Zhimin Yang ,&nbsp;Xin Hu ,&nbsp;Tong Su","doi":"10.1016/j.tice.2024.102583","DOIUrl":"10.1016/j.tice.2024.102583","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the expression and role of methyltransferase 3 (METTL3) in oral malignant transformation.</div></div><div><h3>Materials and methods</h3><div>Immunohistochemical method was used to investigate the expression of METTL3 in the human oral malignant transformation. Bioinformatics analysis was used to explore the role of METTL3 in oral malignant transformation. Oral cancer animal model was used to verify the expression trend of METTL3 in oral malignant transformation. Knockdown of METTL3 expression in human oral mucosal precancerous lesion cells was performed to explore the METTL3 effect on proliferation, migration, apoptosis, and cell cycle.</div></div><div><h3>Results</h3><div>METTL3 expression was significantly up-regulated in the human oral malignant transformation. Moreover, METTL3 was related to the pathway of “Neuroactive ligand-receptor interaction.” In addition, METTL3 expression was also significantly up-regulated in the hamster oral malignant transformation. Finally, the proliferation and migration abilities of human oral mucosal precancerous lesion cells were inhibited after METTL3 knockdown.</div></div><div><h3>Conclusions</h3><div>In conclusion, we found that METTL3 was up-regulated in oral malignant transformation, and the role may relate to the pathway of “Neuroactive ligand-receptor interaction.”</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fragmented polycaprolactone electrospun nanofiber in a hyaluronic acid hydrogel on fibroblasts","authors":"Seo Young Kim,&nbsp;Karthika Muthuramalingam,&nbsp;Hyun Jong Lee","doi":"10.1016/j.tice.2024.102582","DOIUrl":"10.1016/j.tice.2024.102582","url":null,"abstract":"<div><div>Hyaluronic acid (HA) hydrogels have shown promise as biomaterials for soft tissue engineering applications due to their biocompatibility and ability to mimic the extracellular matrix (ECM). However, their limited cell adhesion properties and the need for improved crosslinking methods have hindered their widespread use. In this study, we developed an ECM-mimicking HA hydrogel reinforced with alkaline hydrolyzed (1 M NaOH) fragmented (1.5 cm×1.5 cm) electrospun polycaprolactone (PCL) fibers to enhance cell adhesion and mechanical properties of HA hydrogel. Formation of HA hydrogel was achieved through a thiol-ene click reaction, which is initiated by exposure to visible blue light-activated biocompatible photoinitiator, riboflavin phosphate. The incorporation of alkaline hydrolyzed PCL fiber fragments (PFF) (0 %, 0.1 %, and 1 % w/v) into HA hydrogel precursor solution significantly increased the mechanical stiffness of the HA hydrogel, with the storage modulus ranging from 18.6 ± 0.7 Pa to 216.0 ± 38.2 Pa. The cytocompatibility of the PCL fiber-reinforced HA hydrogel was evaluated using NIH/3T3 fibroblasts. The results demonstrated improved cell adhesion, proliferation, and enhanced cellular functions, including increased production of glycosaminoglycans (GAGs) and collagen, in the PCL fiber-reinforced HA hydrogel compared to the control HA hydrogel. These findings suggest that the developed PCL fiber-reinforced HA hydrogel system, with tunable mechanical properties and excellent cytocompatibility, has potential applications in soft tissue engineering and regenerative medicine.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory effects of oxytocin on normal human cultured melanocyte proliferation, migration, and melanogenesis","authors":"Mohammed M. Alanazi ,&nbsp;Sary Alsanea ,&nbsp;Ashok Kumar ,&nbsp;Zeyad Alehaideb ,&nbsp;Sabine Matou-Nasri ,&nbsp;Khalid M. AlGhamdi","doi":"10.1016/j.tice.2024.102579","DOIUrl":"10.1016/j.tice.2024.102579","url":null,"abstract":"<div><div>Melanocytes are specialized melanin-producing neural crest-derived cells. Melanocyte proliferation and melanin production (i.e., melanogenesis) are crucial for determining skin color. Disruption of these processes can cause pigmentary skin disorders, including hypo-pigmentary disorders such as vitiligo and hyper-pigmentary disorders such as melasma. Understanding these processes is important for discovering new targets to tackle these skin disorders. Therefore, this study aimed to investigate the effects of oxytocin (OXT) on melanocyte functions. Normal Human Cultured Melanocytes (NHCM) were treated with different OXT doses to investigate OXT effects and mechanisms on NHCM proliferation, migration, and on melanogenesis. OXT significantly increased NHCM proliferation and migration in a dose-dependent manner after 72 h of treatment. In addition, OXT dose-dependently upregulated melanogenesis-related microphtalmia-associated transcription factor, tyrosinase, tyrosinase-related protein (TYRP)-1, and TYRP-2 expression accompanied by an increased trend in melanosome number and maturation stage. Furthermore, OXT at concentrations (62.5–125 nM) increased melanin production. These findings suggest the involvement of OXT receptor (OXTR). In addition, this study demonstrates that OXT stimulates melanocyte proliferation, migration, with a tendency toward melanosome maturation, while it modulates melanin production in a dose-dependent manner. Thus, OXT system including its receptor OXTR may be a potential therapeutic target for skin pigmentary disorders.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}