Tissue & cell最新文献_第2页

NERP-1 modifications in amyotrophic lateral sclerosis.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-02-06 DOI: 10.1016/j.tice.2025.102780

B Noli, G Borghero, M M Mascia, Mustafa Hkir, M Puligheddu, C Cocco

{"title":"NERP-1 modifications in amyotrophic lateral sclerosis.","authors":"B Noli, G Borghero, M M Mascia, Mustafa Hkir, M Puligheddu, C Cocco","doi":"10.1016/j.tice.2025.102780","DOIUrl":"10.1016/j.tice.2025.102780","url":null,"abstract":"VGF peptides, such as NERPs (neuroendocrine regulatory peptides 1 and 2), are derived from amino acids 282-306 and 313-350, respectively, of the human proVGF, which is produced in spinal cord motor neurons. Although certain VGF-derived peptides are changed in ALS, less is known about NERPs. Possible modulations of NERPs and additional VGF peptides (NAPP and TPGH) were investigated using specific antibodies through competitive ELISA in the plasma of ALS patients (at both the initial and advanced phases; n = 46 each vs. 46 controls). As additional controls, naïve PD patients were also enrolled (n = 19 vs. 18 controls) while the potential VGF peptide role in oxidative stress was investigated using a motoneuron-like cell line (NSC34) stressed with sodium arsenate (SA). Western blot (WB) and sephadex chromatography (SC) were used to identify the molecular weight (MW) forms recognized by the VGF antibodies. Exclusively NERP-1 immunoreactivity was changed (elevated) in all plasma samples of ALS patients (compared to controls). Therefore, the NERP-1 antibody was the sole antibody used in ELISA with PD samples and NSC-34 cells. No alterations were seen in PD samples (vs. controls) while NERP-1 immunoreactivity decreased within SA-treated cells but increased in their culture medium. The viability test performed by adding NERP-1 to the stressed cells showed no protective effect. Using WB and SC, we revealed NERP-1 antibody reactivity against various MW forms, including those compatible with the NERP-1 peptide and/or proVGF. NERP-1 is suggested as a possible ALS blood biomarker.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102780"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hua Yu Xiao Zheng decoction induces ectopic endometrial stromal cell senescence via inhibiting the PI3K/AKT signaling.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-01-30 DOI: 10.1016/j.tice.2025.102763

Yuan Xu, Yuhui Li, Jingyong Zhang, Pingping Cai

{"title":"Hua Yu Xiao Zheng decoction induces ectopic endometrial stromal cell senescence via inhibiting the PI3K/AKT signaling.","authors":"Yuan Xu, Yuhui Li, Jingyong Zhang, Pingping Cai","doi":"10.1016/j.tice.2025.102763","DOIUrl":"10.1016/j.tice.2025.102763","url":null,"abstract":"Background: Our purpose is to explore the influence of Hua Yu Xiao Zheng (HYXZ) decoction on the senescence of ectopic endometrial stromal cells (ESCs) in endometriosis as well as its relevant mechanisms.Methods: The proliferation, apoptosis, migration, and invasion of primary ectopic ESCs was assessed utilizing EdU assay, flow cytometry, and transwell assays. Moreover, the effects of HYXZ decoction on oxidative stress were evaluated using DCFH-DA probe and via measuring MDA, GSH, SOD and GPx levels. The function of HYXZ decoction on cell senescence were explored through utilizing SA-β-gal staining and measuring the cumulative population doubling level and the average telomere length. The protein expression was measured using western blotting analysis. Endometriosis in rats was surgically induced, and the efficacy and mechanism of HYXZ decoction were determined in vivo.Results: HYXZ decoction inhibited the growth, migration, and invasion of ectopic ESCs, but induced oxidative stress and senescence. HYXZ decoction inhibited PI3K/AKT signaling in ectopic ESCs. PI3K-AKT signaling pathway activator (740Y-P) significantly reversed the inhibitory effects of HYXZ decoction on ectopic ESCs. In rats with endometriosis, oral administration of HYXZ decoction inhibited lesion volume alone with the increased oxidative stress and cell senescence, as well as the decreased PI3K/AKT activity.Conclusion: HYXZ decoction might repress the growth and migration of ectopic ESCs, and induce oxidative stress and senescence through suppressing PI3K/AKT signaling. This finding indicates that HYXZ decoction may be a potential therapeutic drug against endometriosis.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102763"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anwulignan alleviates IRI by the activation of Nrf2/HO-1 signaling pathway and inhibiting NLRP3-caspase-1-GSDMD-mediated pyroptosis in rats.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-02-06 DOI: 10.1016/j.tice.2025.102775

Xin Wang, Shihui Wu, Yuxin Jiang, Zihao Yuan, Jian Liu, Shu Jing, Jiale Liu, Jinghui Sun, Chunmei Wang, Dan Wang, He Li

{"title":"Anwulignan alleviates IRI by the activation of Nrf2/HO-1 signaling pathway and inhibiting NLRP3-caspase-1-GSDMD-mediated pyroptosis in rats.","authors":"Xin Wang, Shihui Wu, Yuxin Jiang, Zihao Yuan, Jian Liu, Shu Jing, Jiale Liu, Jinghui Sun, Chunmei Wang, Dan Wang, He Li","doi":"10.1016/j.tice.2025.102775","DOIUrl":"10.1016/j.tice.2025.102775","url":null,"abstract":"Ischemia/reperfusion injury (IRI) is caused by the reduced blood flow and oxygen level due to the renal artery blockage. The effect of Schisandrae Sphenantherae Fructusandra fruit anwulignan (AN) on the renal IRI injury in rats was investigated. Four rat (Male SD) groups were set, including sham, IRI, sham+AN and IRI+AN groups. This experiment confirmed that AN could reduce renal IRI injury by detecting some biomarkers such as Cre, BUN, LDH, HIF-1α, KIM-1, NGAL, and AIM, which showed decreased levels. AN could increase GSH, CAT, T-AOC, and SOD levels, and decrease MDA and ROS levels in rat kidney tissue, demonstrating that AN can improve oxidative stress damage. In addition, AN diminished the total quantity of TNF-α, IL-1β, IL-6, IL-8, and IL-18 in the renal tissue of rats. In rats with renal IRI, the contents of p-Nrf2 and HO-1 proteins engaged in the Nrf2/HO-1 antioxidant controlled system were increased, and the expression level of Keap1 was diminished. NLRP3, ASC, Caspase-1, GSDMD, GSDMD-N, IL-18, and IL-1β protein levels in kidney tissues decreased significantly in AN group. The results indicate that AN can alleviate renal IRI by reducing the oxidative stress damage via activating the Nrf2/HO-1 signaling pathway and inhibiting NLRP3-Caspase-1-GSDMD-mediated pyroptosis in rats.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102775"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Tau and Amyloid-beta in autophagy gene dysregulation through oxidative stress.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-01-28 DOI: 10.1016/j.tice.2025.102765

Mehrnaz Haghi, Raheleh Masoudi, Fatemeh Ataellahi, Reza Yousefi, Seyed Morteza Najibi

{"title":"Role of Tau and Amyloid-beta in autophagy gene dysregulation through oxidative stress.","authors":"Mehrnaz Haghi, Raheleh Masoudi, Fatemeh Ataellahi, Reza Yousefi, Seyed Morteza Najibi","doi":"10.1016/j.tice.2025.102765","DOIUrl":"10.1016/j.tice.2025.102765","url":null,"abstract":"Background: Alzheimer's disease (AD) is a prevalent neurodegenerative disorder characterized by memory impairment and cognitive decline. Our previous research has demonstrated that pathological Tau and Amyloid-beta (Aβ) disrupt autophagy gene expression, independently. Other studies have shown that these pathological aggregates create a vicious cycle with oxidative stress.Methods: In the current research, the effect of Tau and Amyloid-beta was compared on behavioral function, autophagy gene dysfunction, and oxidative stress in the Drosophila model for AD. Thymoquinone (TQ), an antioxidant agent, was then tested to examine if it could ameliorate the adverse effects of Tau and Amyloid-beta. In addition, the impact of TQ on Tau aggregation was investigated in vitro.Results: Our data showed that Tau and Amyloid-beta induced behavioral disability, autophagy gene dysregulation, and oxidative stress. TQ treatment significantly improved conditions in both types of transgenic flies, with a more profound alleviation in Tau transgenic flies, despite tau having a greater impact on autophagy gene dysregulation. Furthermore, TQ prevented the aggregation of Tau in vitro.Conclusion: To sum up, Tau may exert its toxic effect on autophagy and behavioral dysfunctions significantly through oxidative stress while Amyloid-beta may confer its toxicity through multiple pathways, including oxidative stress. Moreover, since TQ ameliorates the adverse effect of tau and amyloid beta, it could be considered a promising approach for treating AD, probably in combination with other medications against Aβ or Tau.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102765"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The concomitant tumor suspension cells derived from SKBR-3 exhibit circulating tumor cell features.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-02-05 DOI: 10.1016/j.tice.2025.102777

Daiye Li, Feihong Liu, Shanshan Li, Xiaoli Zhao, Kelvin Wai Kwok Yeung, Tak Man Wong, Jun Wu

{"title":"The concomitant tumor suspension cells derived from SKBR-3 exhibit circulating tumor cell features.","authors":"Daiye Li, Feihong Liu, Shanshan Li, Xiaoli Zhao, Kelvin Wai Kwok Yeung, Tak Man Wong, Jun Wu","doi":"10.1016/j.tice.2025.102777","DOIUrl":"10.1016/j.tice.2025.102777","url":null,"abstract":"Metastatic diseases are the major causes of cancer related deaths. Circulating tumor cells are important mediators for distant metastases. However, knowledge about circulating tumor cells is still limited due to their small quantity, lack of explicit markers, interferences from blood cells and immune cells, and so on. In this study, we discovered the concomitant tumor suspension cells in a human epidermal growth factor receptor 2 enriched type breast cancer cell line, SKBR-3. In vitro cultured SKBR-3 shed suspension cells in a spontaneous and continuous manner, which can survive and proliferate infinitely under suspension state. We therefore established the \"progeny\" suspension cell line of its adherent counterpart, or so-called the concomitant tumor suspension cell line. The concomitant tumor suspension cells were in an intermediate partial-epithelial-mesenchymal transition state and were highly adapted to survival in the blood circulation system. The tendency to form microtumors suggests that they are closely related to the metastases of cancers. This study provides a new direction for investigating metastases. By screening more cancer cell lines and establishing more concomitant tumor suspension cell lines, we can acquire much more knowledge implying the evolution of circulating tumor cells, and achieve a better understanding of cancer metastases.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102777"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The clinical significance and biological function of tropomyosin 3 in ulcerative colitis.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-01-31 DOI: 10.1016/j.tice.2025.102770

Xue-Qin Zhang, Jian-Mei Li, Feng-Qian Wang, Yan-Hui Ren, Shi-Xian Wu, Yao Wu, Yuan Tang

{"title":"The clinical significance and biological function of tropomyosin 3 in ulcerative colitis.","authors":"Xue-Qin Zhang, Jian-Mei Li, Feng-Qian Wang, Yan-Hui Ren, Shi-Xian Wu, Yao Wu, Yuan Tang","doi":"10.1016/j.tice.2025.102770","DOIUrl":"10.1016/j.tice.2025.102770","url":null,"abstract":"Background: Ulcerative colitis (UC) is a lifelong chronic inflammatory disease that is characterized by the absence of specific markers for diagnosis and prognosis. TPM3 is an integral component of the thin filament, responsible for the structural stability of actin filaments and modulation of cytoskeletal function. This study investigated the regulatory role of TPM3 in UC and its potential mechanisms.Methods: At the clinical level, TPM3 levels were assessed in serum and mucosal tissues of UC and other enteric disease. At the cellular level, the effects of TMP3 overexpressing lentivirus on Caco-2 cell phenotype and the barrier of IL-1β-induced UC model were explored. At the animal level, the effects of TMP3 overexpressing lentivirus on symptoms and colonic damage in a DSS-induced UC model were explored.Results: TPM3 expression in serum of UC patients was significantly lower than that of other enteric disease, and TPM3 levels in the intestinal mucosa showed a negative correlation with the Mayo score of UC patients. TPM3 overexpression alleviates IL-1β-induced apoptosis and inhibition of invasion and migration in UC model in vitro. In monolayer Caco-2 cells, TPM3 overexpression rescued the IL-1β-induced decrease in transepithelial electrical resistance and tight junction markers (ZO-1 and Occludin) and increase in permeability. In animal experiments, TPM3 overexpression increased body weight and colon length and decreased disease activity index in a DSS-induced UC model. In tissue staining, it alleviated pathological damage and upregulated Occuludin and TPM3 levels in the colon.Conclusion: TPM3 levels correlated with UC disease course and TPM3 overexpression alleviated symptoms/phenotypes and barrier damage in UC models in vivo and in vitro. TPM3 may serve as a potential novel biomarker for UC diagnosis and prognosis.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102770"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143410972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of angiotensin type 2 receptor-mediated nitric oxide pathway in angiotensin II-induced vasorelaxation: Roles of potassium channels.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-01-28 DOI: 10.1016/j.tice.2025.102761

Chinar M Mohammed, Omar A M Al-Habib

{"title":"Molecular mechanisms of angiotensin type 2 receptor-mediated nitric oxide pathway in angiotensin II-induced vasorelaxation: Roles of potassium channels.","authors":"Chinar M Mohammed, Omar A M Al-Habib","doi":"10.1016/j.tice.2025.102761","DOIUrl":"10.1016/j.tice.2025.102761","url":null,"abstract":"A variety of biological functions is attributed to the renin-angiotensin system (RAS). One of them is regulating vascular tone through its final effector Angiotensin II (Ang II). Ang II action is mediated by the Angiotensin type 1 receptor (AT1-R) which plays a role in vasoconstriction, and Angiotensin type 2 receptor (AT2-R) which may result in vascular relaxation through the releasing of endothelium mediates relaxation factors such as Nitric Oxide (NO). Therefore, this study investigated the role of AT2-R in vasodilation after blocking the effect of AT1-R in the rat aorta. Furthermore, it is to determine whether or not Ang II through NO has a role in rat aorta dilation via using valsartan. For control isolated aortic rings were preincubated with Valsartan (AT1- R inhibitor) and then stimulated with angiotensin II dose-dependent. For treating aortic rings different blockers and inhibitors were used. Pd123177 (AT2- R inhibitor) (20 µM), an inhibitor of PKA H-89 (10 µM), eNOS inhibitor L-NAME (0.3 mM), with group of K channel blockers such as TEA (1 mM), 4-AP (1 mM), BaCl2 (1 mM), clotrimazole (0.03 mM) and GLIB (0.01 mM). Our analysis demonstrates vasodilation in aortic rings induced by Ang II after blocking ATI-R and this response was highly reliant on PKA/eNOS and cyclic guanosine monophosphate (cGMP). The data from this investigation provided evidence that Ca2 + activated K+ channels (KCa) and Voltage-dependent K channel (KV) mediated Ang II vasorelaxation. Finally, these results indicate that angiotensin II primarily induces dilatation AT2-R after inhibiting the angiotensin AT1 receptor through a cascade of signaling pathways involving many enzymes and plasma membrane protein channels.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102761"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxifolin mitigates cisplatin-induced testicular damage by reducing inflammation, oxidative stress, and apoptosis in mice.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-02-01 DOI: 10.1016/j.tice.2025.102767

Alayn' Al-Marddyah A Al-Khawalde, Mohammad H Abukhalil, Osama Y Althunibat, Fatima A Jaber, Fatima S Alaryani, Alaa M Saleh, Aishah E Albalawi, Reem H Alhasani

{"title":"Taxifolin mitigates cisplatin-induced testicular damage by reducing inflammation, oxidative stress, and apoptosis in mice.","authors":"Alayn' Al-Marddyah A Al-Khawalde, Mohammad H Abukhalil, Osama Y Althunibat, Fatima A Jaber, Fatima S Alaryani, Alaa M Saleh, Aishah E Albalawi, Reem H Alhasani","doi":"10.1016/j.tice.2025.102767","DOIUrl":"10.1016/j.tice.2025.102767","url":null,"abstract":"Cisplatin (CIS) is effective against various cancers but causes significant side effects, including testicular damage. This study investigated the effects of taxifolin (TX), a potent flavonoid with well-known benefits, against CIS-induced testicular injury. Mice received TX (25 and 50 mg/kg) orally for 14 days, with a single injection of CIS (7 mg/kg) on day 8. CIS significantly impaired sperm parameters (motility, viability, and count) and caused notable histopathological alterations in testicular tissue. CIS-treated testicular tissue exhibited elevated MDA and protein carbonyl levels, alongside decreased antioxidant defenses, including GSH, SOD, and catalase activities. TX significantly mitigated the deterioration of sperm parameters and prevented testicular tissue damage. It also restored antioxidant levels and reduced MDA and protein carbonyl contents. Furthermore, CIS elevated pro-inflammatory markers (NF-κB p65, TNF-α, and IL-1β) and apoptosis markers (Bax and caspase-3), while reducing anti-apoptotic Bcl-2 levels. TX effectively suppressed NF-κB activation, reduced pro-inflammatory cytokine production, and inhibited apoptosis in CIS-treated mice. Overall, TX alleviated CIS-induced oxidative stress, inflammation, apoptosis, and testicular damage, thereby improving sperm quality. These findings emphasize TX's potential as a protective agent against CIS-induced testicular damage and warrant further research in human applications.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102767"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CLMP increases 5-fluorouracil sensitivity in colorectal cancer through the inhibition of autophagy.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-01-30 DOI: 10.1016/j.tice.2025.102771

Kun Yu, Hongjiang Pu, Xuan Zhang, Quan Yang, Weimin Wang, Wenliang Li, Ziyu Li

{"title":"CLMP increases 5-fluorouracil sensitivity in colorectal cancer through the inhibition of autophagy.","authors":"Kun Yu, Hongjiang Pu, Xuan Zhang, Quan Yang, Weimin Wang, Wenliang Li, Ziyu Li","doi":"10.1016/j.tice.2025.102771","DOIUrl":"10.1016/j.tice.2025.102771","url":null,"abstract":"Background: We aimed to explore the biological function of CLMP in colorectal cancer (CRC) and to determine the effect of CLMP on 5-fluorouracil (5-FU) sensitivity in CRC.Methods: Sixteen pairs of CRC tissues and paracancerous tissues were collected. Immortalized intestinal epithelial cell lines and human CRC cell lines were purchased, and the cells were treated with DMSO and 5-FU. RTqPCR, western blotting, CCK8, colony formation, scratch, and Transwell assays were performed to determine the molecular mechanism of CLMP in the regulation of autophagy and sensitivity to 5-FU in CRC cells.Results: CLMP was expressed at low levels in CRC tissues. The upregulation of CLMP expression could inhibit cell proliferation, colony number, migration and invasion and increase the sensitivity of CRC cells to 5-FU. Mechanistic studies revealed that the overexpression of CLMP could block the activation of the PI3K/AKT signaling pathway, inhibit autophagy, and increase the chemosensitivity of CRC cells to 5-FU.Conclusion: CLMP overexpression can reduce the level of autophagy and increase the sensitivity of CRC to 5-FU, providing a potential target for the treatment of CRC.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102771"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural changes in the ganglionic layer of the rat cerebellar cortex due to the use of monosodium glutamate and sodium nitrite in combination.

IF 2.7 4区生物学

Tissue & cell Pub Date : 2025-04-01 Epub Date: 2025-02-07 DOI: 10.1016/j.tice.2025.102760

Bohdan Kononov, Serhii Bilash, Ihor Tretiak, Maryna Kononova, Olena Pronina, Mykhailo Koptev, Angelina Pirog-Zakaznikova, Svitlana Donchenko, Yaryna Oliinichenko, Vladyslav Oleksiienko

{"title":"Structural changes in the ganglionic layer of the rat cerebellar cortex due to the use of monosodium glutamate and sodium nitrite in combination.","authors":"Bohdan Kononov, Serhii Bilash, Ihor Tretiak, Maryna Kononova, Olena Pronina, Mykhailo Koptev, Angelina Pirog-Zakaznikova, Svitlana Donchenko, Yaryna Oliinichenko, Vladyslav Oleksiienko","doi":"10.1016/j.tice.2025.102760","DOIUrl":"10.1016/j.tice.2025.102760","url":null,"abstract":"Background: The issue of using many food additives in food is becoming increasingly relevant. The effect of these substances on the nervous system, namely the cerebellum, is not unrelated. There are studies on the impact of food additives individually, but their combined effect has not been studied sufficiently. Therefore, the aim of our study was to determine structural changes in rats' ganglionic layer of the cerebellar cortex under the influence of monosodium glutamate and sodium nitrite in combination.Methods: The experiment involved 84 white Wistar laboratory rats, which were divided into a control group and five experimental groups. The obtained cerebellar samples were paraffin-embedded and histological sections (3-4) μm thick were made. These sections were stained with hematoxylin, eosin, and silver impregnated by Grimmelius.Results: After calculating the average thickness of the cerebellar cortex ganglionic layer at different administration periods of monosodium glutamate and sodium nitrite in combination, significant changes were observed after week 1, where this indicator was 1.18 times less than in the control. Also, a decrease in the average thickness was observed after the 4th and 12th and a significant decrease in the 16th week of the study, namely by 1.61 times, 1.43 times and 1.77 times, respectively. It indicates substantial structural changes in the ganglionic layer.Conclusions: The study found that the ganglionic layer is formed of a single row of Purkinje cells, and they, in turn, are the main functional link in the entire grey matter of the cerebellum, which suggests that the use of a complex of food additives causes functional disorders of the cerebellum as a whole.","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"102760"},"PeriodicalIF":2.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0