Tissue & cell最新文献

{"title":"The effects of sofosbuvir on the colon of adult male albino rats and the potential protective role of propolis: A histological and immunohistochemical study","authors":"Eman S. El-Roghy ,&nbsp;Eman A. Eissa","doi":"10.1016/j.tice.2026.103373","DOIUrl":"10.1016/j.tice.2026.103373","url":null,"abstract":"<div><h3>Background</h3><div>Although sofosbuvir is a promising remedy for chronic hepatitis C virus infection, it presents some side effects. Propolis is a natural product that provides a solution to mitigate the risk. This study aims to illuminate the impact of sofosbuvir on the colon, for the first time to our knowledge, and the role of propolis.</div></div><div><h3>Methods</h3><div>Forty adult male albino rats were divided equally into four groups and were given drugs as a single daily dose for four weeks. Group I (distilled water). Group II (200 mg/kg propolis). Group III (40 mg/kg sofosbuvir). Group IV (sofosbuvir + propolis). At the end of the experiment, body weight was measured, and biochemical analysis of oxidative stress markers was done. After animal scarification, colon specimens were prepared and stained by Hematoxylin and Eosin, Masson's trichrome, Alcian blue, Cyclooxygenase-2 (COX-2), and proliferating nuclear antigen (PCNA) immunostaining. Scanning electron microscopic examination was done.</div></div><div><h3>Results</h3><div>Propolis co-administration revealed a significant decrease in crypt damage and total colitis, depth of mucosal fissures, and collagen fiber deposition (P &lt; 0.001). Conversely it displayed a significant increase in goblet cell numbers compared to the sofosbuvirtreated group (P &lt; 0.001). This positive effect of propolis is provided via the considerable reduction in malondialdehyde, and by a rise in superoxide dismutase and glutathione (P &lt; 0.001). Propolis co-treatment displayed a significant downregulation of COX-2 and PCNA immunoreaction compared to the sofosbuvir-treated group (P &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>Propolis protected against colitis caused by sofosbuvir, regulating oxidation, inflammation, and cell proliferation.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103373"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146189720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative multi-omics profiling implicates purine metabolism and the PI3K-Akt pathway in radiation-induced lung injury","authors":"Jun Qiu ,&nbsp;Xinpan Li ,&nbsp;Yi Fang ,&nbsp;Qiaoyuan Wu ,&nbsp;Shixiong Liang","doi":"10.1016/j.tice.2026.103330","DOIUrl":"10.1016/j.tice.2026.103330","url":null,"abstract":"<div><h3>Background</h3><div>Thoracic radiotherapy commonly causes radiation-induced lung injury (RILI); however, its mechanisms remain poorly defined, and no FDA-approved therapies are available.</div></div><div><h3>Methods</h3><div>To identify key pathways and regulators, we analyzed metabolic and transcriptomic alterations in a murine model of RILI. Following a 15 Gy thoracic irradiation, lung tissues were assessed by histopathology (H&amp;E and Masson staining), LC-MS-based metabolomics, and transcriptomics.</div></div><div><h3>Results</h3><div>Irradiated lungs showed erythrocyte exudation, inflammatory infiltration, fibrosis, and epithelial damage, whereas control tissues showed no pathological changes. Metabolomic profiling identified 186 differentially abundant metabolites, primarily enriched in purine and glycine/serine/threonine metabolism. Transcriptomics revealed 180 differentially expressed genes. Integrative analysis demonstrated that both metabolites and genes converged on the PI3K–Akt and phospholipase D signaling pathways, suggesting their potential as early RILI biomarkers. Analyses also revealed a consistent association between RILI and lung microbiota dysbiosis, pointing to novel mechanistic insights.</div></div><div><h3>Conclusions</h3><div>Our integrative multi-omics analysis reveals that RILI progression is characterized by dysregulated purine metabolism and transcriptomic changes that converge on the PI3K-Akt and phospholipase D signaling pathways. These pathway alterations were associated with lung microbiota dysbiosis, providing new avenues for early biomarker discovery and therapeutic intervention.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103330"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146039545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The METTL14/YTHDF3 axis regulates EPSTI1-mediated osteogenic differentiation of human bone marrow mesenchymal stem cells","authors":"Changjun Zheng ,&nbsp;Lingzhi Ding ,&nbsp;Yan Xu ,&nbsp;Chi Yuan ,&nbsp;Bingjie Jiang ,&nbsp;Tingting Chen ,&nbsp;Yongjie Wang","doi":"10.1016/j.tice.2026.103386","DOIUrl":"10.1016/j.tice.2026.103386","url":null,"abstract":"<div><h3>Background</h3><div>Osteoporosis is a disease characterized by impaired bone microarchitecture and reduced bone mass. However, its molecular mechanisms are not fully understood.</div></div><div><h3>Methods</h3><div>Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blot (WB) were used to detect the expression of messenger RNA (mRNA) and proteins. Flow cytometry was used to quantify cell surface markers. Cell transfection was used for gene knockdown and overexpression. Cell counting kit-8 (CCK-8) and 5-ethynyl-2’-deoxyuridine (EdU) were used to assess cell viability and proliferation. Alkaline phosphatase (ALP) staining and alizarin red S (ARS) staining were used to evaluate ALP activity and osteogenic differentiation. RNA modification variant database 2.0 (RMVar 2.0), RNA modification base database 3.0 (RMbase V3.0), and sequence-based RNA adenosine methylation site predictor (SRAMP) were used to predict RNA modifications. RNA-protein interaction (RPI) database was used to predict RNA-protein interactions. RNA immunoprecipitation (RIP) and methylated RNA immunoprecipitation (MeRIP) were used to detect RNA-protein binding and RNA chemical modifications. Actinomycin D (Act D) was used to study mRNA stability. The encyclopedia of RNA interactomes (ENCORI) was used to predict interactions among RNA molecules.</div></div><div><h3>Results</h3><div>Epithelial-stromal interaction 1 (EPSTI1) was highly expressed in osteoporosis. Knockdown of EPSTI1 promoted hBMSC viability, proliferation, and osteogenic differentiation, whereas its overexpression produced the opposite effects. Methyltransferase-like 14 (METTL14) and YTH N6-methyladenosine RNA binding protein 3 (YTHDF3) co-regulated the N6-methyladenosine (m⁶A) modification of EPSTI1. METTL14 overexpression enhanced hBMSC viability, proliferation, and osteogenic differentiation, but simultaneous overexpression of EPSTI1 partially offset these promoting effects.</div></div><div><h3>Conclusion</h3><div>METTL14 and YTHDF3 regulate EPSTI1 expression through its m⁶A modification, thereby modulating proliferation and osteogenic differentiation of hBMSCs.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103386"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146190128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological architecture of the developing pancreas in quail embryos: A histological and ultrastructural perspective","authors":"Fatma Abdelhakeem ,&nbsp;Elsayed S.I. Mohammed ,&nbsp;Mohammed Al-Rasheed ,&nbsp;Fatma A. Madkour","doi":"10.1016/j.tice.2026.103344","DOIUrl":"10.1016/j.tice.2026.103344","url":null,"abstract":"<div><div>Organogenesis of the avian pancreas is a critical developmental process that ensures proper digestive and metabolic functions in birds. This study investigated the morphogenesis and ultrastructural differentiation of the quail pancreas. Fifty-eight quail embryos from day 5 to day 17 of incubation were used for this study. The developing pancreas consistently composed of exocrine and endocrine portions. On day 5, primitive pancreatic tubules (duct-like structures) appeared within the duodenal mesenchyme, lined with pseudostratified and simple columnar epithelium, and associated with early endocrine clusters representing the islets of Langerhans. By day 6, the pancreas was enclosed by a delicate capsule, and the pancreatic cells became cuboidal to pyramidal with prominent basally located nuclei. Progressive lobulation occurred from days 8 and 9, forming dorsal, ventral, third, and splenic lobes with increased vascularization and telocytes distribution. On 10th day of incubation, four distinct lobes were established, and each lobe had its own capsule on 14th day. By day 17, mature serous acini exhibited basophilic basal cytoplasm and apical zymogen granules, indicating functional secretory activity. Ultrastructurally, exocrine acinar cells displayed abundant rough endoplasmic reticulum, prominent nuclei, and diverse zymogen granules. Centroacinar and intercalated ductal cells formed the initial ductal network. Telocytes appeared in perivascular and interacinar regions, suggesting a regulatory role in tissue organization. Endocrine islets were made of α-, β-, and δ-cells, each possessing distinct secretory granules corresponding to glucagon, insulin, and somatostatin secretion, respectively. Grossly, the pancreas developed progressively between the duodenal limbs and attained distinct lobular organization by hatching. These findings elucidate the prehatching structural maturation of the quail pancreas, establishing a foundation for understanding avian pancreatic development and function.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103344"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146067256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming osimertinib resistance in NSCLC: The role of Notch1 pathway inhibition by berberine","authors":"Xiaozhen Jiang ,&nbsp;Xia Wen ,&nbsp;Jiajia Fu","doi":"10.1016/j.tice.2026.103365","DOIUrl":"10.1016/j.tice.2026.103365","url":null,"abstract":"<div><h3>Background</h3><div>Resistance acquired during treatment with third-generation EGFR-TKIs, including osimertinib, continues to pose a significant obstacle in the management of advanced non-small cell lung cancer (NSCLC). Dysregulation of the Notch1 signaling pathway plays a critical role in tumor development as well as therapeutic resistance. Berberine (BER), a naturally derived bioactive compound, has been reported to exert diverse antitumor effects across multiple cancer types.</div></div><div><h3>Methods</h3><div>We investigated Notch1 signaling in osimertinib-resistant NSCLC cells and assessed the effects of combined berberine and osimertinib treatment on epithelial-mesenchymal transition (EMT) and drug sensitivity.</div></div><div><h3>Results</h3><div>Aberrant Notch1 activation promoted EMT and contributed to osimertinib resistance. Berberine inhibited Notch1 signaling, reversed EMT in resistant cells, and restored sensitivity to osimertinib with minimal toxicity.</div></div><div><h3>Conclusion</h3><div>Berberine overcomes osimertinib resistance by targeting the Notch1 pathway and reversing EMT, providing a novel strategy to enhance EGFR-TKI efficacy in NSCLC and supporting integration of traditional Chinese medicine with targeted therapies.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103365"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146189723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the mechanism of DLK1 in placenta of intrahepatic cholestasis of pregnancy","authors":"Zhizun Li ,&nbsp;Xiaomei Huang ,&nbsp;Yong Shao ,&nbsp;Qin Tang","doi":"10.1016/j.tice.2026.103350","DOIUrl":"10.1016/j.tice.2026.103350","url":null,"abstract":"<div><h3>Background</h3><div>Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific disorder, prominently characterized by elevated serum bile acids and adverse fetal outcomes. This study focused on the expression of Delta-like 1(DLK1) in the placenta of patients with ICP,along with the alterations in placental blood vessels and function. The bile acid - induced ICP cell model was utilized to clarify the impact of DLK1 on the function of trophoblast cells under the pathological context of ICP.</div></div><div><h3>Methods</h3><div>Human placental tissues were subjected to immunohistochemical staining, immunofluorescence, Western blotting (WB)and qPCR. These techniques were employed to analyze the expression of DLK1 and functional status of blood vessels in the ICP placenta. An ICP cell model was established using Taurocholate acid (TCA). WB, qPCR, and reactive oxygen species (ROS) assays were conducted to detect the effects of TCA and DLK1 on trophoblast function. The effects of TCA and DLK1 on endothelial cell function were evaluated through migration and tube formation assays.</div></div><div><h3>Results</h3><div>The results indicated that the expression levels of DLK1, placental growth factor, and angiogenesis factor were significantly lower in the human ICP placenta. Concomitantly, the Notch1 signaling pathway was found to be inhibited. The addition of DLK1 to the ICP cell model improved the function of the cells, and the supernatant of the trophoblast cells also promoted the migration and tube formation ability of HUVECs.</div></div><div><h3>Conclusions</h3><div>In the ICP placenta, the expression of DLK1 is reduced, accompanied by a decline in placental blood vessels and placental function. Exogenous addition of DLK1 can activate the Notch1 pathway, promote placental angiogenesis and mitigate oxidative damage.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103350"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological characteristics of the lung in Eurasian teal (Anas crecca): Insights from cross-sectional, casting, histological, and SEM analyses","authors":"Alaa Abd El Moneam ,&nbsp;Ashraf El Sharaby ,&nbsp;Asmaa Aboelnour ,&nbsp;Mohamed M.A. Abumandour ,&nbsp;Ahmed G. Nomir","doi":"10.1016/j.tice.2026.103353","DOIUrl":"10.1016/j.tice.2026.103353","url":null,"abstract":"<div><div>This study detailed a comprehensive morphological characterization of the adult Eurasian teal (<em>Anas crecca</em>) lungs through anatomical techniques, including gross anatomy, cross-sectional analysis, corrosion casting, histology, and scanning electron microscopy (SEM). Twenty lungs were examined to identify their topographical position, structural organization, and microarchitecture. Gross anatomical examination showed trapezoidal, non-lobated lungs located craniodorsally within the thoracoabdominal cavity, showing distinct surfaces and borders with seven intercostal tori. Corrosion casting revealed the branching pattern of the bronchial system, highlighting the intrapulmonary primary bronchus (IPPB) and its secondary bronchi, including medioventral (MVSBs) and laterodorsal (LVSBs), interconnected by parabronchi (PB). Seven laterodorsal secondary bronchi (LDSBs) and four lateroventral secondary bronchi (LVSBs) were identified, with posterior secondary bronchi (POSBs) also present along the lateral wall of the IPPB. The POSBs were the shortest, while the medioventral secondary bronchi were the largest. SEM examination showed the spatial arrangement of elongated paleo- and hexagonal neo-parabronchi, characterized by hexagonal architecture and atrial openings leading to air capillaries. Histological examination confirmed the presence of pseudostratified ciliated epithelium in primary and secondary bronchi, smooth muscle layers, and reticular fiber-rich septa separating parabronchi. Two types of hexagonal parabronchi were identified: longitudinally arranged in the paleopulmonic region and circular in the neopulmonic region. The interatrial septa were lined with squamous to cuboidal epithelial cells. These findings enhance understanding of avian respiratory morphology and provide baseline data for comparative anatomical and functional studies in waterfowl species.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103353"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Morphological Analysis Of The Small Intestine In The Pekin Duck's (Anas platyrhynchos) Using Gross Anatomical, Histological, Histochemical, and Scanning Electron Microscopic Techniques","authors":"Youssef Elsabbgh ,&nbsp;Ashraf El Sharaby ,&nbsp;Asmaa Aboelnour ,&nbsp;Mohamed M.A. Abumandour ,&nbsp;Ahmed G. Nomir","doi":"10.1016/j.tice.2026.103346","DOIUrl":"10.1016/j.tice.2026.103346","url":null,"abstract":"<div><div>The adult White Pekin duck’s small intestine is anatomically adapted for rapid growth, high-fat diet digestion, and strong immunity, essential for its physiological needs. This study analyzes the adaptive anatomical features of the small intestine—duodenum, jejunum, and ileum—through gross examination, light microscopy (LM), histochemical techniques, scanning electron microscopy (SEM), and morphometric analysis. To achieve this objective, sixteen ducks were examined. Gross examination revealed a U-shaped duodenum, a coiled jejunum with Meckel’s diverticulum, and a straight ileum attached to paired ceca. Histologically, the duodenum displayed finger-like villi with the deepest crypts, while the jejunum had the tallest villi and the highest villus/crypt ratio. The ileum showed compact villi and the highest goblet cell (GC) density. GCs were quantified and differentiated using PAS, AB, and PAS-AB combination staining. PAS-positive (neutral mucins) cells predominated in villi, especially in the jejunum, while AB-positive (acidic mucins) cells were most abundant in ileal crypts, indicating regional specialization in mucosal protection. SEM revealed ultrastructural features including dense microvilli, epithelial exfoliation, and GC secretory vesicles. Enterocytes exhibited organelles such as mitochondria and rough endoplasmic reticulum, as well as junctional complexes including tight junctions and desmosomes. These findings highlight the segmental specialization of the Pekin duck’s small intestine, with the jejunum optimized for nutrient absorption and the ileum for immune defense. The detailed mucin profile and ultrastructural features provide insight into the digestive efficiency and adaptive physiology of this fast-growing avian species.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103346"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing mesenchymal stem/stromal cells from dermis discarded in elective surgeries: Identifying optimal sources for regenerative medicine","authors":"Helena Debiazi Zomer ,&nbsp;Adriane Cristina Fagundes ,&nbsp;Lais Andrade Ferreira ,&nbsp;Augusto Cesar Spadaccia Asciutti ,&nbsp;Priscilla Barros-Delben ,&nbsp;Maiara Marques da Silva ,&nbsp;Julia Mitze ,&nbsp;Talita da Silva Jeremias ,&nbsp;Andrea Gonçalves Trentin","doi":"10.1016/j.tice.2026.103352","DOIUrl":"10.1016/j.tice.2026.103352","url":null,"abstract":"<div><div>The dermis is a valuable and accessible source of mesenchymal stem/stromal cells (MSCs), particularly for applications in skin wound healing. Dermal MSCs (DSCs) can be isolated from skin fragments typically discarded during routine elective surgeries, such as facelifts (rhytidectomies), tummy tucks (abdominoplasties), and circumcisions (postectomies). While MSCs generally share core stem cell properties, their behavior may be influenced by factors such as donor age, sex, and the anatomical tissue origin. Understanding the specific characteristics of DSCs from different sources is therefore crucial for identifying the most suitable cells for clinical use. In this study, we compared human DSCs obtained from rhytidectomies (postmenopausal facial dermis), abdominoplasties (adult abdominal dermis), and postectomies (prepubertal foreskin dermis) by assessing their isolation efficiency, stemness markers, proliferative capacity, and in vitro wound healing. All three DSC populations exhibited comparable stemness profiles; however, DSCs from prepubertal foreskin demonstrated superior isolation yield, expansion potential, and colony-forming efficiency, likely reflecting the younger age of the tissue donors. Despite these differences, DSCs from both prepubertal foreskin and adult abdominal skin exhibited similar wound healing performance in vitro. In conclusion, our findings indicate that the source of dermal tissue significantly influences DSC behavior in vitro, with cells derived from postectomies and abdominoplasties showing enhanced potential compared to those from rhytidectomies.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103352"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose-derived stem cell exosomes attenuated fibroblast senescence by regulating endoplasmic reticulum stress through SIRT1","authors":"Yagang Ding ,&nbsp;Bangde Xue ,&nbsp;Mingkui Gao ,&nbsp;Sibin Guan ,&nbsp;Qinchuan Li ,&nbsp;Jun Liu","doi":"10.1016/j.tice.2026.103345","DOIUrl":"10.1016/j.tice.2026.103345","url":null,"abstract":"<div><div>Adipose-derived stem cells are widely used in aging field because of their extensive sources, low immunogenicity and strong secretory function. In particular, the exosomes of adipose-derived stem cells are rich in small molecules of RNA and protein. Studies have shown that exosomes also play a role in regulation of ER stress. Therefore, we hypothesized that exosomes of adipose-derived stem cells could regulate ER stress and delay fibroblasts senescence. In this study, we use H₂O₂ to promote cellular senescence and treat with adipose-derived stem cell exosomes. The function of cell proliferation and apoptosis were compared, and the levels of validating factor and collagen were detected. Then we explored the mechanism of action of exosomes derived from adipose-derived stem cells. We found adipose-derived stem cell exosomes could inhibit fibroblast senescence, including promoting cell proliferation, inhibiting apoptosis and regulating collagen secretion, by regulating ER stress through SIRT1. To further validate the relevance of this mechanism at the population level, public human skin transcriptomic data were analyzed, revealing coordinated downregulation of SIRT1 expression and unfolded protein response (UPR) signaling during physiological skin aging, with a strong positive correlation between SIRT1 expression and UPR pathway activity. This study suggests that adipose-derived stem cell exosomes attenuate stress-induced senescence-like changes in fibroblasts, highlighting their potential relevance in the modulation of cellular aging.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"100 ","pages":"Article 103345"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}