Tissue & cell最新文献

{"title":"Mechanisms of Complanatoside A against extracellular matrix accumulation, inflammation and proliferation of mesangial cells in diabetic nephropathy through network pharmacology and experimental validation","authors":"Weiyu Han ,&nbsp;Zhewen Deng ,&nbsp;Jiaqi Li,&nbsp;Beiting Ma,&nbsp;Zhengxin Lu,&nbsp;Yimu Zhang,&nbsp;Chaoxing Ren,&nbsp;Bo Ma","doi":"10.1016/j.tice.2025.103031","DOIUrl":"10.1016/j.tice.2025.103031","url":null,"abstract":"<div><div>Diabetic nephropathy (DN) is the leading cause of end-stage renal disease (ESRD) worldwide, underscoring the urgent need to explore effective strategies for its prevention and control. Accumulating evidence indicates that inhibition of abnormal mesangial cells (MCs) proliferation, particularly during the early stages of DN, represents a critical therapeutic strategy for attenuating the progression of DN. Complanatoside A (CA), a major flavonoid derived from <em>Semen Astragali Complanati</em>, has demonstrated significant reno-protective properties. In this study, diabetic mouse models and high-glucose (HG)-induced mouse mesangial cell models were established to investigate the reno-protective mechanisms of CA. Furthermore, network pharmacology was employed to predict molecular targets and signaling pathways. Histopathological analysis revealed that CA alleviated key renal pathological changes, including glomerular interstitial fibrosis, thickening of the glomerular basement membrane, mesangial matrix expansion, glomerulosclerosis, and fibrillar collagen deposition. Furthermore, CA inhibited HG-induced mesangial extracellular matrix (ECM) accumulation, inflammatory responses, and cellular proliferation. Through network pharmacology, eight core genes (TNF-α, AKT1, HSP90AA1, MMP9, PPARG, SRC, PTGS2, and MMP2) were identified as critical targets of CA in DN and were primarily associated with inflammatory responses and ECM deposition. Molecular docking analysis confirmed that CA exhibited high binding affinity for these inflammation- and ECM-related genes. This study demonstrated that CA effectively mitigated renal injury in DN by suppressing inflammatory pathways and ECM deposition, thus providing novel insights into its therapeutic potential. These findings offer new perspectives for the development of traditional Chinese medicine-based interventions for the treatment of DN.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103031"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical administration of Juniperus polycarpos K. Koch extract triggered wound healing by decreasing inflammation and accelerating cell proliferation in diabetic rat model","authors":"Amirsaeed Hosseini ,&nbsp;Emran Habibi ,&nbsp;Samira Mirzaeizade ,&nbsp;Reza Talaei ,&nbsp;Hossein Masoudi ,&nbsp;Davood Nasiry","doi":"10.1016/j.tice.2025.103032","DOIUrl":"10.1016/j.tice.2025.103032","url":null,"abstract":"<div><div>One of the primary difficulties for individuals with diabetes is the development of chronic wounds. The current research sought to investigate the potential antioxidant and anti-inflammatory properties of <em>Juniperus polycarpos</em> K. Koch (JP) extract when topically applied in diabetic wound healing. A total of 75 diabetic rats were randomly assigned into the control group, eucerin group, 2 % <em>Juniperus polycarpos</em> K. Koch ointment (JP 2 %) group, 5 % <em>Juniperus polycarpos</em> K. Koch ointment (JP 5 %) group, and phenytoin group. Skin samples were obtained on days 7, 14, and 21 to assess them through histological, molecular, and biomechanical methods. The results indicated that in the treatment groups, there was a significant enhancement in the rate of wound healing, the volumes of newly formed epidermis and dermis, the overall number of fibroblasts and blood vessels, collagen deposition, and biomechanical parameters compared to the control and eucerin groups, with the most pronounced changes observed in the JP 5 % group. Furthermore, there was a significant rise in the transcripts of TGF-β and VEGF genes in the treatment groups compared to the control and eucerin groups, with the highest levels of expression seen in the JP 5 % group. In the JP 5 % group, there was a significantly larger reduction in TNF-α and IL-1β expression, along with a decrease in neutrophil count, compared to the other groups. These findings confirmed that the topical application of JP extract, particularly at a 5 % concentration, significantly accelerated the healing of diabetic wounds.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103032"},"PeriodicalIF":2.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protection of naringenin against 5-fluorouracil toxicity in the liver","authors":"Ibrahim Aktas ,&nbsp;Sedat Bilgiç ,&nbsp;Fatih Mehmet Gur","doi":"10.1016/j.tice.2025.103023","DOIUrl":"10.1016/j.tice.2025.103023","url":null,"abstract":"<div><div>Drugs used in cancer treatment eliminate cancer cells but also have toxic effects on healthy cells. The study aims to determine the protective effect of the antioxidant naringenin (NAR) against the harmful effects of 5-fluorouracil (5-FU) used in cancer treatment on the liver. A total of 28 Sprague-Dawley male rats were divided into four groups (n = 7) as control, 5-FU, NAR and 5-FU + NAR. 150 mg/kg 5-FU was administered intraperitoneally to the 5-FU and 5-FU + NAR groups. 100 mg/kg NAR was administered to the NAR and 5-FU + NAR groups via a gavage catheter. At the end of the experiment, biochemical analyses performed on the blood serum taken from the animals showed that 5-FU increased the levels of liver oxidative stress markers. Aspartate aminotransferase (AST), high-density lipoprotein (HDL), total cholesterol (TG), alanine aminotransferase (ALT), triglycerides (CH) levels increased in the blood (p &lt; 0.05). It was found that glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels decreased and malondialdehyde (MDA) levels increased in liver tissue due to the effect of 5-FU (p &lt; 0.03). It was also confirmed that the situation was normalized as a result of the combined application of 5-FU and NAR. Histopathological examinations showed that 5-FU caused karyopyknosis and microvesicular steatosis in hepatocytes. Immunohistochemical examinations showed that 5-FU increased the expression of caspase 3 and TNF-α in the liver. NAR, which has antioxidant, antiapoptotic and anti-inflammatory effects, largely prevented the pathological formations that developed with 5-FU application. According to the results of the study, NAR was shown to protect against the harmful effects that may occur in the liver during treatment with 5-FU.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103023"},"PeriodicalIF":2.7,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constitutively active Akt-mediated cellular senescence inhibits apoptosis in adenocarcinoma of the esophagogastric junction","authors":"Naomi Fukagawa ,&nbsp;Akihiro Murayama ,&nbsp;Ako Yokoi ,&nbsp;Yasuko Oguri ,&nbsp;Miki Hashimura ,&nbsp;Yohei Harada ,&nbsp;Chika Kusano ,&nbsp;Makoto Saegusa","doi":"10.1016/j.tice.2025.103030","DOIUrl":"10.1016/j.tice.2025.103030","url":null,"abstract":"<div><div>The mechanisms of initiation and progression of adenocarcinoma of the esophagogastric junction (AEG) are largely unclear. To elucidate such mechanisms, we looked for correlations between histopathological and immunohistochemical features of Siewert type II AEG cases and gastric non-cardia carcinoma (GNCC) cases and AEG clinicopathological data. MKN74 gastric cancer cells stably overexpressing active Akt (myr-Akt), inactive Akt (MAA-Akt), and mutant (mt) β-catenin (mt-β-cat) were also used. Overall survival but not disease-free survival was worse for AEG compared to GNCC. This correlated with significantly lower cleaved PARP1 scores, less apoptotic figures, and higher levels of phospho (p) Akt, pGSK-3β, and nuclear β-catenin. myr-Akt cells exhibited senescence-like features, along with increased mouse double minute-2 (MDM2) expression and p53-independent stabilization of p21^waf1; this was consistent with the high p21^waf1 score and frequent mutant (mt) p53 status in AEG as compared to GNCC. Moreover, myr-Akt cells treated with cisplatin (CDDP) displayed less apoptotic features and had a high BCL2:BAX ratio; the converse was observed in MAA-Akt cells. Finally, mt-β-cat cells exhibited senescent features and were sensitive to CDDP-induced apoptosis, independently of Akt and GSK-3β status. In conclusion, constitutively active Akt induces cellular senescence through p53-independent stabilization of p21^waf1, which leads to sustained high BCL2 expression and protects against apoptosis. Together with the GSK-3β/β-catenin axis and high MDM2 expression, this contributes to AEG progression.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103030"},"PeriodicalIF":2.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical evaluation and characterization of normal perioral tissues with a new advanced videodermatoscopy method: A cross-sectional pilot study on dental students","authors":"Andrea Camodeca ,&nbsp;Antonino Fiorino ,&nbsp;Romeo Patini ,&nbsp;Gioele Gioco ,&nbsp;Francesca Colacino ,&nbsp;Carlo Lajolo ,&nbsp;Massimo Cordaro","doi":"10.1016/j.tice.2025.103021","DOIUrl":"10.1016/j.tice.2025.103021","url":null,"abstract":"<div><h3>Aims</h3><div>The main outcome is to describe the key dermatomucosal structures of the healthy perioral tissues and to assess their visibility and ease of identification, using an innovative advanced dermatoscopic technique.</div></div><div><h3>Methods</h3><div>this pilot observational cross-sectional clinical study was conducted to evaluate and describe the characteristics of healthy perioral tissue in 40 young-adult subjects. For all subjects, two lightweight probes were used, with different magnifications. For each patient, three anatomical areas of interest were identified: the anterior chin area (ACA); the transition zone between the ACA and the labial integument, also known as the vermilion border (VB), and the pigmented area of the lip, also known as the vermilion zone (VZ). These were evaluated with both white light and polarized light. The acquired dermatoscopic and fluorescence-advanced videodermatoscopy (FAV) images were carefully reviewed by two independent investigators. In addition to a descriptive phase focusing on the normal anatomy of various regions, a qualitative assessment of the visibility of the mucocutaneous structures was performed. Image analysis utilized the visibility scale.</div></div><div><h3>Results</h3><div>Visibility scores significantly differed between lighting modes and magnification levels. Skin texture was better visualized with non-polarized light (p &lt; 0.001), while polarized light improved visualization of pores and vascular structures (p &lt; 0.01). No significant differences emerged by gender, skin type, or phototype.</div></div><div><h3>Conclusion</h3><div>these findings highlight the capability of FAV technology to provide detailed visualization of both epithelial and non-epithelial structures, with consistent patterns of visibility across different anatomical zones.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103021"},"PeriodicalIF":2.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of ERK/MAPK signaling by MAZ through transcriptional repression of PPP3CA to promote osteoclastogenesis and osteoporosis progression: Functions and mechanisms","authors":"Xinying Shang ,&nbsp;Wei Hu ,&nbsp;Lin Zhang ,&nbsp;Rui Wei","doi":"10.1016/j.tice.2025.103028","DOIUrl":"10.1016/j.tice.2025.103028","url":null,"abstract":"<div><h3>Objective</h3><div>Osteoporosis (OP) is a systemic skeletal disease that contributes to compromised bone strength and increased fracture risk. This paper investigates the functional role of protein phosphatase 3 catalytic subunit α (PPP3CA) in OP and its mechanisms.</div></div><div><h3>Methods</h3><div>Bioinformatics prediction analysis was performed to identify genes associated with OP and its transcription factor. An OP mouse model was constructed by ovariectomy (OVX). RAW264.7 cells were induced to differentiate into osteoclasts using RANKL for <em>in vitro</em> experiments.</div></div><div><h3>Results</h3><div>PPP3CA was poorly expressed in the femoral tissues of OVX-induced mice. Overexpression of PPP3CA reduced nuclear factor of activated T cells c1 (NFATC1) and matrix metalloproteinase 9 (MMP9) levels and the extent of extracellular signal-regulated protein kinase (ERK)1/2 phosphorylation. Overexpression of PPP3CA inhibited osteoclast differentiation and OP progression. Myc-associated zinc finger protein (MAZ) was highly expressed in the femoral tissues of OVX-induced mice and inhibited the transcription of PPP3CA by binding to its promoter. MAZ promoted the ERK/mitogen-activated protein kinase (MAPK) pathway and thus promoted osteoclastogenesis. Animal experiments further demonstrated that the MAZ/PPP3CA axis promoted osteoclastogenesis through the ERK/MAPK pathway <em>in vivo</em>.</div></div><div><h3>Conclusion</h3><div>MAZ promotes osteoclastogenesis and OP progression by activating the ERK/MAPK signaling pathway through transcriptional inhibition of PPP3CA. These findings not only elucidate a novel mechanism driving osteoclastogenesis but also highlight the MAZ/PPP3CA/ERK-MAPK axis as a promising target for innovative therapies aimed at curbing bone resorption and ultimately improving treatment outcomes in OP.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103028"},"PeriodicalIF":2.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble α-Klotho protects dermal microvascular endothelial cells against endothelial-to-mesenchymal transition","authors":"Eloisa Romano ,&nbsp;Irene Rosa ,&nbsp;Eleonora Sgambati ,&nbsp;Bianca Saveria Fioretto ,&nbsp;Mirko Manetti","doi":"10.1016/j.tice.2025.103029","DOIUrl":"10.1016/j.tice.2025.103029","url":null,"abstract":"<div><div>Endothelial-to-mesenchymal transition (EndMT) is a key contributor to dermal fibrosis. The soluble form of the α-Klotho (sKL) hormone has been shown to counteract fibrotic processes in multiple organs, but its role in dermal fibrosis and EndMT remains unexplored. To investigate whether sKL may inhibit transforming growth factor β1 (TGFβ1)-induced EndMT in human dermal microvascular endothelial cells (H-dMVECs), cells pretreated with recombinant human sKL and subsequently stimulated with recombinant human TGFβ1 were assessed for morphological changes, gene and protein expression of both endothelial and mesenchymal/myofibroblast markers, and functional contractility through qPCR, Western blotting, immunofluorescence, and collagen gel contraction assays, respectively. TGFβ1-treated H-dMVECs underwent significant changes in cell morphology, with loss of endothelial markers (i.e., CD31 and VE-cadherin) and a concomitant increase in the expression of mesenchymal/myofibroblast markers (i.e., α-smooth muscle actin, type I collagen, and S100A4/fibroblast-specific protein 1) and of EndMT-associated transcription factors (Snail1, Twist1, and Zeb1). Moreover, TGFβ1-treated H-dMVECs acquired the ability to contract collagen gel matrices. Pretreatment with sKL significantly attenuated all the aforementioned morphological, molecular, and functional changes, preserving the endothelial phenotype and mitigating myofibroblast-like contractile activity. In conclusion, sKL effectively prevented TGFβ1-induced EndMT in H-dMVECs, highlighting its potential as a novel therapeutic agent against dermal fibrosis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103029"},"PeriodicalIF":2.7,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oleic acid inhibits the proliferation, invasion, and migration of tongue squamous cell carcinoma cells under high glucose conditions through S100A9-HK2/PKM2-SOD2 signaling pathway","authors":"Yujie Ma ,&nbsp;Runqiang Liu ,&nbsp;Juan Zhan ,&nbsp;Yihan Lai ,&nbsp;Sicheng Li ,&nbsp;Wei Wang ,&nbsp;Yisen Shao ,&nbsp;Lin Jiang","doi":"10.1016/j.tice.2025.103026","DOIUrl":"10.1016/j.tice.2025.103026","url":null,"abstract":"<div><div>The incidence of tongue squamous cell carcinoma (TSCC) has been increasing annually. Type 2 diabetes mellitus (T2DM) is one of the risk factors for TSCC, with hyperglycemia being a hallmark of T2DM that promotes the development of oral cancer and results in poorer prognoses for TSCC patients who also have T2DM. Oleic acid (OA), a component of Brucea javanica oil emulsion, exhibits both anti-cancer and anti-diabetic properties. However, the precise regulatory mechanisms of OA on tongue squamous cell carcinoma (TSCC) under high glucose conditions have yet to be fully elucidated. Our findings demonstrated that high glucose significantly enhanced the migratory, invasive, and proliferative capabilities of TSCC cells. Notably, OA treatment effectively reversed these high glucose-induced enhancements in TSCC cellular activities. Furthermore, our research revealed that OA exerted its anti-tumor effects by stabilizing its binding with S100A9, which modulated the glycolytic pathway. Ultimately, we confirmed that the anti-tumor mechanism of OA in TSCC patients with T2DM was associated with the inactivation of the S100A9-HK2/PKM2-SOD2 pathway. In summary, OA inhibited the proliferation, migration, and invasion of TSCC cells under high glucose conditions through the regulation of the S100A9-HK2/PKM2-SOD2 pathway.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103026"},"PeriodicalIF":2.7,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in 3D bioprinting of functional biomaterials for neural tissue engineering","authors":"Shengji Li ,&nbsp;Xianyu Meng ,&nbsp;Zixin Zhong ,&nbsp;Changqing Li","doi":"10.1016/j.tice.2025.103024","DOIUrl":"10.1016/j.tice.2025.103024","url":null,"abstract":"<div><div>3D bioprinting has emerged as a transformative technology in neural tissue engineering, enabling the precise fabrication of complex, biomimetic scaffolds to address challenges in neural repair. This review synthesizes recent advancements in 3D bioprinting technologies, focusing on their integration with functional biomaterials to enhance neural regeneration. We systematically analyze key technical aspects, including inkjet, extrusion, and laser - assisted printing modalities, emphasizing their capabilities in constructing neural guidance scaffolds with tailored mechanical properties and bioactivity. Functional biomaterials such as natural polymers (alginate, gelatin), synthetic polymers (PLA, PVA), and bioceramics are evaluated for their roles in promoting cell adhesion, differentiation, and neurotrophic factor delivery. The application of 3D bioprinting in peripheral nerve injury repair, spinal cord injury treatment, and neurodegenerative disease modeling is critically reviewed, highlighting preclinical successes and translational potential. Current bottlenecks, including printing precision, material stability, and clinical scalability, are discussed alongside future directions such as smart responsive materials and AI - integrated bioprinting systems. This work underscores the synergy between advanced biomaterials and 3D bioprinting as a promising avenue for developing personalized neural therapies.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103024"},"PeriodicalIF":2.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bushen Jiangu decoction alleviates osteoporosis by inhibiting MMP2 expression and activation of the MAPK pathway in vitro","authors":"Pengyuan Chen,&nbsp;Jiong Lin","doi":"10.1016/j.tice.2025.103025","DOIUrl":"10.1016/j.tice.2025.103025","url":null,"abstract":"<div><h3>Objectives</h3><div>To explore the precise mechanism of BJD against osteoporosis.</div></div><div><h3>Methods</h3><div>The active compounds and drug targets of BJD were screened from public databases and identified by HPLC. Differentially expressed genes related to osteoporosis were obtained, followed by overlapping targets of BJD and osteoporosis identified by PPI networks. Then the binding interactions between active compounds and hub targets were validated by molecular docking vina followed by molecular dynamics simulations. The effects and mechanisms of BJD against osteoporosis was validated through <em>in vitro</em> experiments.</div></div><div><h3>Results</h3><div>Three core active compounds in BJD were obtained. Molecular docking highlighted the affinity of the three compounds towards PTGS2, MMP2, and ADORA2B. In the LPS-induced MC3T3-E1 osteoblast cells, BJD significantly promoted cell viability and revived ALP activity. BJD reduced the expression of MMP2 and PTGS2 whereas elevated ADORA2B expression in the LPS-induced osteoblasts. BJD could alleviate osteoporosis by alleviating the activation of the MAPK signaling. Elevated levels of MMP2 impaired the migration capacity, and significantly increased ALP, COLI, OCN and OPN expression levels in LPS-induced MC3T3-E1 cells after BJD treatment, as well as the osteoblast calcium nodules were incompletely formed and deposited.</div></div><div><h3>Conclusion</h3><div>BJD alleviated osteoporosis by inhibiting MMP2 expression and activation of MAPK signaling.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103025"},"PeriodicalIF":2.7,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144491082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}