Tissue & cell最新文献_第3页

Decellularization of various tissues and organs through chemical methods 通过化学方法使各种组织和器官脱细胞。

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-05 DOI: 10.1016/j.tice.2024.102573

Tayebeh Sadat Tabatabai , Majid Salehi , Leila Rezakhani , Zohreh Arabpour , Ali R. Djalilian , Morteza Alizadeh

{"title":"Decellularization of various tissues and organs through chemical methods","authors":"Tayebeh Sadat Tabatabai , Majid Salehi , Leila Rezakhani , Zohreh Arabpour , Ali R. Djalilian , Morteza Alizadeh","doi":"10.1016/j.tice.2024.102573","DOIUrl":"10.1016/j.tice.2024.102573","url":null,"abstract":"<div><div>Due to the increase in demand for donor organs and tissues during the past 20 years, new approaches have been created. These methods include, for example, tissue engineering in vitro and the production of regenerative biomaterials for transplantation. Applying the natural extracellular matrix (ECM) as a bioactive biomaterial for clinical applications is a unique approach known as decellularization technology. Decellularization is the process of eliminating cells from an extracellular matrix while preserving its natural components including its structural and functional proteins and glycosaminoglycan. This can be achieved by physical, chemical, or biological processes. A naturally formed three-dimensional structure with a biocompatible and regenerative structure is the result of the decellularization process. Decreasing the biological factors and antigens at the transplant site reduces the risk of adverse effects including inflammatory responses and immunological rejection. Regenerative medicine and tissue engineering applications can benefit from the use of decellularization, a promising approach that provides a biomaterial that preserves its extracellular matrix.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization 含溴结构域 4 的抑制剂通过调节巨噬细胞极化对抗胃癌前病变

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-02 DOI: 10.1016/j.tice.2024.102580

Lei Zhu , Qingxin Cai , Gang Li , Xiaoming Zou

{"title":"Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization","authors":"Lei Zhu , Qingxin Cai , Gang Li , Xiaoming Zou","doi":"10.1016/j.tice.2024.102580","DOIUrl":"10.1016/j.tice.2024.102580","url":null,"abstract":"<div><h3>Objective</h3><div>Gastric precancerous lesions (GPL), characterized by intestinal metaplasia and dysplasia, marks a pivotal juncture in the transformation from gastritis to gastric cancer. Research on GPL could offer fresh perspectives on preventing cancer occurrence.</div></div><div><h3>Methods</h3><div>This study employed 1-methyl-3-nitro-1-nitrosoguanidine (MNNG) to establish GPL rat models and knocked BRD4 down <em>in vivo</em> to assess its impact on the lesions and macrophage morphology. Following that, the impacts of BRD4 knockdown on the malignant phenotypes of human gastric epithelial GES-1 cells were determined. Moreover, conditioned medium from macrophage was gathered and used for GES-1 cell culture. The involvement of macrophage polarization in the BRD4 regulatory mechanism in GES-1 cells was assessed.</div></div><div><h3>Results</h3><div>This study elucidated that MNNG induced an increase level of BRD4 in the rat models. BRD4 knockdown reduced lesions based on pathological sections and immunohistochemistry to detect proliferative antigens. Western blotting and immunofluorescence showed that BRD4 knockdown suppressed epithelial-mesenchymal transition and macrophage M2 polarization. In in vitro experiments, BRD4 knockdown inhibited the malignant phenotype of GES-1 cells and the differentiation of THP-1 cells into M2 macrophages, respectively. The conditioned medium from M2 macrophages with BRD4 knockdown was co-incubated with GES-1 cells, which attenuated the malignant phenotypes compared with the medium from M2 macrophages.</div></div><div><h3><strong>Conclusion</strong></h3><div>Through in vivo and in vitro experiments, BRD4 upregulation was found to already occur during GPL, affecting macrophage polarization and epithelial cell cancerization. This finding provides an experimental basis for strategies targeting BRD4 inhibition at this critical stage.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomes from adipose-derived stem cells overexpressing microRNA-671–3p promote fat graft angiogenesis and adipogenic differentiation 过表达 microRNA-671-3p 的脂肪来源干细胞外泌体可促进脂肪移植血管生成和成脂分化。

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-10-01 DOI: 10.1016/j.tice.2024.102575

Xiaoyan Hao, Yuan Guo, Xueyuan Yu, Lin He, Youcheng He, Maoguo Shu

{"title":"Exosomes from adipose-derived stem cells overexpressing microRNA-671–3p promote fat graft angiogenesis and adipogenic differentiation","authors":"Xiaoyan Hao, Yuan Guo, Xueyuan Yu, Lin He, Youcheng He, Maoguo Shu","doi":"10.1016/j.tice.2024.102575","DOIUrl":"10.1016/j.tice.2024.102575","url":null,"abstract":"<div><div>Exosomes from adipose-derived stem cells (ADSCs) have been demonstrated to benefit angiogenesis, wound healing, and fat grafting. Small noncoding RNAs such as microRNA (miRNA) and circular RNA play critical roles in mediating the function of ADSCs-derived exosomes. However, the underlying mechanisms have not been fully elucidated. In this study, we investigated the function and mechanism of human ADSCs-derived exosomes (hADSCs-Exo) in promoting fat graft angiogenesis and adipogenic differentiation. hADSCs-Exo were isolated and identified, and treatment with hADSCs-Exo enhanced fat graft angiogenesis and adipogenic differentiation in a mouse fat graft implantation model. We found that hADSCs-Exo overexpressed miR-671–3p and promoted human umbilical vein endothelial cell (HUVEC) proliferation, migration, and invasion. Bioinformatics analysis and luciferase reporter assay validated that TMEM127 is a direct target of miR-671–3p. Rescue experiments demonstrated that TMEM127 overexpression partially antagonized the function of hADSCs-Exo <em>in vitro</em>, such as suppressing HUVEC cell proliferation, migration, and invasion. Moreover, TMEM127 overexpression abrogated the function of hADSCs-Exo on fat graft angiogenesis and adipogenic differentiation. Taken together, our findings demonstrate that miR-671–3p-overexpressing exosomes from ADSC promote fat graft angiogenesis and adipogenic differentiation, which highlights the potential of targeting the ADSC-Exosomes-miR-671–3p/TMEM127 axis to improve outcome of fat graft and tissue engineering regenerative medicine.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cephalomannine reduces radiotherapy resistance in non-small cell lung cancer cells by blocking the β-catenin-BMP2 signaling pathway 头孢甘露碱通过阻断β-catenin-BMP2信号通路降低非小细胞肺癌细胞的放疗耐药性

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-09-30 DOI: 10.1016/j.tice.2024.102577

Suhong An, Xiaoping Xu, Yanhong Bao, Fang Su, Yiqian Jiang

{"title":"Cephalomannine reduces radiotherapy resistance in non-small cell lung cancer cells by blocking the β-catenin-BMP2 signaling pathway","authors":"Suhong An, Xiaoping Xu, Yanhong Bao, Fang Su, Yiqian Jiang","doi":"10.1016/j.tice.2024.102577","DOIUrl":"10.1016/j.tice.2024.102577","url":null,"abstract":"<div><h3>Background</h3><div>The management of non-small cell lung cancer (NSCLC) often includes the use of radiotherapy, with individual outcomes being impacted by the tumor's response to this treatment modality. Cephalomannine (CPM), a taxane diterpenoid found in <em>Taxus</em> spp, has been found to have anti-tumor activity. This study was aim to the explore the role and mechanism by which CPM affects radiotherapy resistance in NSCLC.</div></div><div><h3>Methods</h3><div>H460 cells were pretreated with different doses of CPM. H460 cells were transfected with β-catenin overexpression plasmids. The cell viability, colony-forming ability, migration ability, and sphere-forming ability and apoptosis of the cells were measured by using CCK-8, colony-forming, transwell, and sphere-forming assay and flow cytometry. Western blot assay was employed to detect the expression of β-catenin and BMP2.</div></div><div><h3>Results</h3><div>The cell viability, proliferation, migration and sphere-forming ability of cells in the radiotherapy-resistant (RR) group were significantly higher than those in the radiotherapy-sensitivity (RS) group. Conversely, the apoptosis rate of cells in the RR group was lower than that in the RS group. However, after CPM pretreatment of RR group cells, the above phenomena were reversed in a CPM dose-dependent manner. Subsequently, pretreatment with CPM resulted in a decrease in the expression levels of β-catenin and BMP2 in the RR group. In addition, overexpression of β-catenin mitigated the inhibitory effects of CPM on radiotherapy-resistant NSCLC cells.</div></div><div><h3>Conclusion</h3><div>CPM has the potential to decrease radiotherapy resistance in NSCLC cells by inhibiting the β-catenin-BMP2 signaling pathway, promoting apoptosis, and ultimately impeding cell growth.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interplay of skin architecture and cellular dynamics in wound healing: Insights and innovations in care strategies 伤口愈合过程中皮肤结构和细胞动态的相互作用：护理策略的启示与创新。

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-09-30 DOI: 10.1016/j.tice.2024.102578

Sourav Dhandhi , Yeshna , Vishal , Monika , Bhawna Goel , Samrat Chauhan , Suchitra Nishal , Monika Singh , Vikas Jhawat

{"title":"The interplay of skin architecture and cellular dynamics in wound healing: Insights and innovations in care strategies","authors":"Sourav Dhandhi , Yeshna , Vishal , Monika , Bhawna Goel , Samrat Chauhan , Suchitra Nishal , Monika Singh , Vikas Jhawat","doi":"10.1016/j.tice.2024.102578","DOIUrl":"10.1016/j.tice.2024.102578","url":null,"abstract":"<div><div>Wound healing involves complex interactions among skin layers: the epidermis, which epithelializes to cover wounds; the dermis, which supports granulation tissue and collagen production; and the hypodermis, which protects overall skin structure. Key factors include neutrophils, activated by platelet degranulation and cytokines, and fibroblasts, which aid in collagen production during proliferation. The healing process encompasses inflammation, proliferation, and remodeling, with angiogenesis, fibroplasia, and re-epithelialization crucial for wound closure. Angiogenesis is characterized by the creation of collateral veins, the proliferation of endothelial cells, and the recruitment of perivascular cells. Collagen is produced by fibroblasts in granulation tissue, aiding in the contraction of wounds. The immunological response is impacted by T cells and cytokines. External topical application of various formulations and dressings expedites healing and controls microbial contamination. Polymeric materials, both natural and synthetic, and advanced dressings enhance healing by providing biodegradability, biocompatibility, and infection control, thus addressing tissue regeneration challenges. Numerous dressings promote healing, including films, hydrocolloids, hydrogels, foams, alginates, and tissue-engineered substitutes. Wound dressings are treated with growth factors, particularly PDGF, and antibacterial drugs to prevent infection. The challenges of tissue regeneration and infection control are evolving along with the field of wound care.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attenuation of inflammation, oxidative stress and TGF-β1/Smad3 signaling and upregulation of Nrf2/HO-1 signaling mediate the protective effect of diallyl disulfide against cadmium nephrotoxicity 二烯丙基二硫化物对镉肾毒性的保护作用可抑制炎症、氧化应激和TGF-β1/Smad3信号传导，上调Nrf2/HO-1信号传导

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-09-27 DOI: 10.1016/j.tice.2024.102576

Reem S. Alruhaimi , Emad H.M. Hassanein , Ahmad F. Ahmeda , Sulaiman M. Alnasser , Ahmed M. Atwa , Mostafa Sabry , Mohammed A. Alzoghaibi , Ayman M. Mahmoud

{"title":"Attenuation of inflammation, oxidative stress and TGF-β1/Smad3 signaling and upregulation of Nrf2/HO-1 signaling mediate the protective effect of diallyl disulfide against cadmium nephrotoxicity","authors":"Reem S. Alruhaimi , Emad H.M. Hassanein , Ahmad F. Ahmeda , Sulaiman M. Alnasser , Ahmed M. Atwa , Mostafa Sabry , Mohammed A. Alzoghaibi , Ayman M. Mahmoud","doi":"10.1016/j.tice.2024.102576","DOIUrl":"10.1016/j.tice.2024.102576","url":null,"abstract":"<div><div>Heavy metals are toxic environmental pollutants with serious health effects on humans and animals. Cadmium (Cd) is known for its serious nephrotoxic effect and its toxicity involves oxidative stress (OS) and inflammation. Diallyl disulfide (DADS), a main constituent of garlic, exhibites cytoprotective and antioxidant activities. This study investigated the effect of DADS on OS, inflammation, and fibrosis induced by Cd in rat kidney, pointing to the involvement of transforming growth factor-β (TGF-β)/Smad3 and nuclear factor erythroid 2–related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling, and peroxisome proliferator-activated receptor gamma (PPARγ). Rats received DADS for 14 days and Cd on day 7 and blood and kidney samples were collected. Cd elevated serum creatinine, urea and uric acid, provoked kidney histopathological alterations and collagen deposition, increased kidney malondialdehyde (MDA) level, and decreased glutathione (GSH) and antioxidant enzymes. Nuclear factor-kappaB (NF-κB) p65, interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-1β, and CD68 were upregulated in Cd-administered rat kidney. DADS prevented kidney injury, mitigated OS, suppressed NF-κB, CD68 and pro-inflammatory mediators, and boosted antioxidants. DADS downregulated TGF-β1, Smad3 phosphorylation and Kelch-like ECH-associated protein-1 (Keap1), and increased Nrf2, HO-1, cytoglobin, and PPARγ. In conclusion, DADS protects the kidney against Cd toxicity by attenuating OS, inflammation, and TGF-β1/Smad3 signaling, and enhancement of Nrf2/HO-1 signaling, antioxidants, and PPARγ.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142356891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alleviative effects of green-fabricated zinc oxide nanoparticles on acrylamide-induced oxidative and inflammatory reactions in the rat stomach via modulating gastric neuroactive substances and the MiR-27a-5p/ROS/NF-κB axis 绿色制造的纳米氧化锌颗粒通过调节胃神经活性物质和MiR-27a-5p/ROS/NF-κB轴对丙烯酰胺诱导的大鼠胃氧化和炎症反应具有缓解作用

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-09-25 DOI: 10.1016/j.tice.2024.102574

Yasmina M. Abd-Elhakim , Amany Abdel-Rahman Mohamed , Tarek Khamis , Mohamed M.M. Metwally , Eman S. El-Shetry , Amirah Albaqami , Wedad Mawkili , Manal E. Alosaimi , Badriyah S. Alotaibi , Naira ElAshmouny , Naief Dahran , Ghadi Alsharif , Mai A. Samak

{"title":"Alleviative effects of green-fabricated zinc oxide nanoparticles on acrylamide-induced oxidative and inflammatory reactions in the rat stomach via modulating gastric neuroactive substances and the MiR-27a-5p/ROS/NF-κB axis","authors":"Yasmina M. Abd-Elhakim , Amany Abdel-Rahman Mohamed , Tarek Khamis , Mohamed M.M. Metwally , Eman S. El-Shetry , Amirah Albaqami , Wedad Mawkili , Manal E. Alosaimi , Badriyah S. Alotaibi , Naira ElAshmouny , Naief Dahran , Ghadi Alsharif , Mai A. Samak","doi":"10.1016/j.tice.2024.102574","DOIUrl":"10.1016/j.tice.2024.102574","url":null,"abstract":"<div><div>Little is known about the effects of acrylamide (AMD) on the stomach. So, this study evaluated the effect of oral AMD exposure (20 mg/kg b.wt) on oxidative status, apoptotic, and inflammatory reactions in rat’s stomach for 60 days. To explore novel targets of AMD toxicity, a more detailed molecular and immune-expression study was performed. Besides, the possible protective effect of green synthesized zinc oxide nanoparticles (G-ZNP) (10 mg/kg b.wt) was explored. The results revealed that AMD significantly provoked oxidative and lipid peroxidative damage of the stomach in terms of increased ROS and MDA but reduced SOD, CAT, GSH, and GSH/GSSG. Additionally, the stomachs of AMD-exposed rats showed a significant increment of PGE2 but reduced NO. Histopathologically, AMD induced a significant increase in PAS stain and the immunoexpression of iNOS and NF-κB in the glandular stomach. A significant upregulation of CART, VACHT, EGFR, caspase-3, NOS-1, and miR-27a-5p was evident in the stomach of the AMD group. Yet, G-ZNP oral dosing significantly rescued the AMD-induced oxidative damage, apoptotic reaction, inflammatory effect, and altered miR-27a-5p and gene expressions in the stomach. Conclusively, these findings demonstrated the efficacy of G-ZNP in protecting against the harmful impacts of acrylamide on stomach tissues.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142356978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L‐arginine mitigates choroid plexus changes in Alzheimer’s disease rat model via oxidative/inflammatory burden and behavioral modulation 左旋精氨酸通过氧化/炎症负担和行为调节减轻阿尔茨海默病大鼠模型中脉络丛的变化

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-09-24 DOI: 10.1016/j.tice.2024.102572

Wael Amin Nasr El-Din , Islam Omar Abdel Fattah

{"title":"L‐arginine mitigates choroid plexus changes in Alzheimer’s disease rat model via oxidative/inflammatory burden and behavioral modulation","authors":"Wael Amin Nasr El-Din , Islam Omar Abdel Fattah","doi":"10.1016/j.tice.2024.102572","DOIUrl":"10.1016/j.tice.2024.102572","url":null,"abstract":"<div><div>Aging is a risk factor for Alzheimer’s disease (AD), leading to choroid plexus (CP) alterations. This study aimed to explore the possible therapeutic mechanisms of ARG on AD-induced CP changes. Sprague-Dawley rats were divided into 6 groups (n = 7 per group): adult, adult+ARG, aged, aged+ARG, aged+AD, and aged+AD+ARG groups. Evaluations were for Y-maze test, serum levels of oxidative/inflammatory markers, and serum and cerebrospinal fluid (CSF) markers of AD, histopathology, immunohistochemistry, and histomorphometry. The aged+AD group demonstrated a significant decline in maze test parameters, total antioxidant capacity (TAC), brain-derived neurotrophic factor (BDNF) levels, and vascular endothelial growth factor (VEGF) immunoexpression, while tumour necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), beta-amyloid (Aβ) levels and amyloid protein precursor (APP), and heat shock protein90 (HSP90) immunoexpressions were significantly increased. Sections of this group showed flat epitheliocytes, congested capillaries, connective tissue expansion, and degenerated endothelium. These parameters were modulated by ARG administration, via increased levels of TAC (1.37 vs 2.17 mmol/L), (p = 0.018) BDNF (serum: 48.50 vs 78.41; CSF: 4.07 vs 7.11 pg/ml) (p< 0.001), and VEGF (0.07 vs 0.26 OD) (p< 0.001), in addition to decreased levels of TNF-α (86.63 vs 41.39 pg/ml) (p< 0.001), IL-1β (96.04 vs 39.57 pg/ml) (p< 0.001), Aβ (serum: 67.40 vs 47.30; CSF: 189.26 vs 169.84 pg/ml) (p< 0.001), and HSP90 (0.54 vs 0.13 OD) (p< 0.001). In conclusion, ARG ameliorates the AD-associated CP changes, including histopathological, oxidative/inflammatory, and AD markers, and VEGF and HSP90 immunohistochemical alterations. Dietary ARG consumption is recommended to avoid AD progression in the elderly.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of fetal bovine serum concentration on stemness and neuronal differentiation markers in stem cells from human exfoliated deciduous teeth 胎牛血清浓度对人类脱落牙齿干细胞的干性和神经元分化标记的影响

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-09-24 DOI: 10.1016/j.tice.2024.102571

Mateus de Oliveira Lisboa , Ana Helena Selenko , Agner Henrique Dorigo Hochuli , Alexandra Cristina Senegaglia , Letícia Fracaro , Paulo Roberto Slud Brofman

{"title":"The influence of fetal bovine serum concentration on stemness and neuronal differentiation markers in stem cells from human exfoliated deciduous teeth","authors":"Mateus de Oliveira Lisboa , Ana Helena Selenko , Agner Henrique Dorigo Hochuli , Alexandra Cristina Senegaglia , Letícia Fracaro , Paulo Roberto Slud Brofman","doi":"10.1016/j.tice.2024.102571","DOIUrl":"10.1016/j.tice.2024.102571","url":null,"abstract":"<div><div>Dental Stem Cells (DSCs) from discarded teeth are a non-invasive and ethically favorable source with the potential for neurogenesis due to their ectodermal origin. Stem cells from human exfoliated deciduous teeth (SHED) are particularly promising due to their high differentiation potential and relative immaturity compared to other Mesenchymal Stromal Cells (MSCs). Markers like CD56 and CD271 are critical in identifying MSC subpopulations for therapeutic applications because of their roles in neurodevelopment and maintaining stemness. This study investigates how fetal bovine serum (FBS) concentrations affect the expression of CD56 and CD271 in SHED, influencing their stemness and neuronal differentiation potential. SHEDs were isolated from various donors, cultured, and characterized for MSC traits using markers such as CD14, CD19, CD29, CD34, CD45, CD73, CD90, CD105, CD56, and CD271. Culturing SHED in different FBS conditions (standard 15 %, reduced 1 % and 5 %, and FBS-free) showed that lower FBS concentrations increase CD271 and CD56 expression while maintaining the standard MSC immunophenotype. Importantly, the enhanced expression of these markers can be induced even after SHEDs have been expanded in standard FBS concentrations. These findings suggest that FBS concentration can optimize SHED culture conditions, enhancing their suitability for regenerative medicine and tissue engineering applications.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142356890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomal therapy is a luxury area for regenerative medicine 外泌体疗法是再生医学的一个奢侈领域。

IF 2.7 4区生物学

Tissue & cell Pub Date : 2024-09-21 DOI: 10.1016/j.tice.2024.102570

Nahla A. Hassaan , Hanaa A. Mansour

{"title":"Exosomal therapy is a luxury area for regenerative medicine","authors":"Nahla A. Hassaan , Hanaa A. Mansour","doi":"10.1016/j.tice.2024.102570","DOIUrl":"10.1016/j.tice.2024.102570","url":null,"abstract":"<div><div>Stem cell-based therapies have made significant advancements in tissue regeneration and medical engineering. However, there are limitations to cell transplantation therapy, such as immune rejection and limited cell viability. These limitations greatly impede the translation of stem cell-based tissue regeneration into clinical practice. In recent years, exosomes, which are packaged vesicles released from cells, have shown promising progress. Specifically, exosomes derived from stem cells have demonstrated remarkable therapeutic benefits. Exosomes are nanoscale extracellular vesicles that act as paracrine mediators. They transfer functional cargos, such as miRNA and mRNA molecules, peptides, proteins, cytokines, and lipids, from MSCs to recipient cells. By participating in intercellular communication events, exosomes contribute to the healing of injured or diseased tissues and organs. Studies have shown that the therapeutic effects of MSCs in various experimental paradigms can be solely attributed to their exosomes. Consequently, MSC-derived exosomes can be modified and utilized to develop a unique cell-free therapeutic approach for treating multiple diseases, including neurological, immunological, heart, and other diseases. This review is divided into several categories, including the current understanding of exosome biogenesis, isolation techniques, and their application as therapeutic tools.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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