Tissue & cell最新文献

{"title":"Vernonia Amygdalina restores prednisolone-induced damage to hypothalamic pituitary testicular axis in male wistar rats","authors":"Eniola Risikat Kadir ,&nbsp;Azeezat Dagbo Yakub ,&nbsp;Waliyat Abiola El-Hussein ,&nbsp;Emmanuel Oluwasegun Oluwafemi ,&nbsp;Abdulmaliq Toyin Abdulmaliq ,&nbsp;Aisha Titilayo Yakub ,&nbsp;Ibrahim Ahmad Itopa ,&nbsp;Abdulmumin Ibrahim ,&nbsp;Mubarak Oloduowo Ameen","doi":"10.1016/j.tice.2025.103022","DOIUrl":"10.1016/j.tice.2025.103022","url":null,"abstract":"<div><div>Glucocorticoids, such as prednisolone (PRED), exert inhibitory effects on immune cells. PRED is a commonly administered glucocorticoid that efficiently manages acute inflammatory disease and autoimmune disorders. In immunosuppressive states, infertility is impaired by interference with the control and secretion of reproductive hormones from the hypothalamic-pituitary-gonadal axis. <em>Vernonia amygdalina</em> (VA), a widely consumed vegetable in Africa, has anti-oxidative, anti-malarial, and sperm-improvement properties. The study aims to evaluate <em>the impact of Vernonia amygdalina</em> on reproductive parameters in immunosuppressed Wistar rats. Adult male Wistar rats (160–180 g) were grouped into six randomly (each group had five rats), totalling thirty in number. Control was Group A, which had distilled water administered; Group B was administered 2 mg/kg PRED for 28days; and other groups, C, were administered 2 mg/kg PRED for 28days, then treatment was withdrawn for 28days; Group D received 2 mg/kg PRED for 28days, then 50 mg/kg levamisole for 56days and E and F were treated with 2 mg/kg PRED for 28days, then 250 mg/kg and 375 mg/kg of bitter leaf respectively for 56days. All animals were euthanized 24 h after the last dosing by administration of ketamine (80 mg/kg). The study's main findings reveal the promising potential of Vernonia amygdalina to restore reproductive health in immunosuppressed rats. An increase in WBC and haemoglobin, with associated alterations in the antioxidant system, was observed in animals given prednisolone only. Sperm motility, count and morphology significantly decreased in the immunosuppressed group compared to the controls. Low-dose bitterleaf increased normal sperm count, morphology and motility compared to controls. Similarly, increased IBA-1-positive cells in the hypothalamus indicated increased oxidative damage. <em>Vernonia amygdalina</em> administration effectively restored sperm parameters and oxidative damage induced by prednisolone in the hypothalamic pituitary testicular axis. These findings open up new possibilities for future research and potential clinical applications, instilling a sense of hope and optimism in the audience.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103022"},"PeriodicalIF":2.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Icariin mitigates experimental contrast-induced nephropathy: Mechanistic insights","authors":"Hanan A. Elgendy ,&nbsp;Amira Osman ,&nbsp;Amira E. Farage ,&nbsp;Medhat Taha ,&nbsp;Sara Abubakr ,&nbsp;Alaa.M. Badawy ,&nbsp;Mohie Mahmoud Ibrahim ,&nbsp;Ahmed Nabawy Ahmed Nasr ,&nbsp;Hala Mahfouz ,&nbsp;Tourki A.S. Baokbah ,&nbsp;Ahmed shaban Abdelmonsef ,&nbsp;Mohamed Ezzat Mahmoud ,&nbsp;Ahmed Abdel-monem Elmetwally ,&nbsp;Abdulrahman I. AbuAqil ,&nbsp;Nora Elshehawy Helal","doi":"10.1016/j.tice.2025.103020","DOIUrl":"10.1016/j.tice.2025.103020","url":null,"abstract":"<div><h3>Background</h3><div>Currently, the use of contrast agents in interventional procedures and imaging has been increasing. Icariin, an active component of <em>Epimedium</em>, offers beneficial biological activities such as antioxidation and anti-inflammation. Therefore, our study aimed to investigate the protective role of icariin and its underlying mechanisms against contrast-induced nephropathy (CIN).</div></div><div><h3>Methods</h3><div>We divided twenty-four rats into four groups (n = 6 each): control group icariin-only treated group, contrast-induced nephropathy (CIN) group, and CIN+Icariin group, with six rats in each group. We performed renal function tests (serum creatinine and blood urea nitrogen (BUN) and histological evaluations. Additionally, we measured malondialdehyde (MDA) and superoxide dismutase (SOD) levels in renal supernatant and used ELISA to quantify caspase-8, caspase-9, cytochrome c, Bcl-2 associated protein x (Bax), and B cell lymphoma/leukemia 2 protein (Bcl-2) levels. We assessed nuclear factor kappa beta (NF-κB) and interleukin-1 beta (IL-1β) gene expression and evaluated immunoexpression of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), CD68, and caspase-3.</div></div><div><h3>Results</h3><div>Icariin showed nephroprotective effects against CIN, demonstrated by reduced creatinine and BUN; decreased MDA, IL-6, NF-κB, TNF-α, IL-1β, CD68, caspase-8, caspase-9, Bax, and cytochrome c; and increased SOD and Bcl-2 levels. Renal histology improved in the CIN+Icariin group compared to CIN group.</div></div><div><h3>Conclusions</h3><div>Oral administration of 100 mg/kg icariin exhibited renoprotective effects against contrast-induced renal injury through anti-inflammatory, anti-apoptotic, and antioxidant mechanisms.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103020"},"PeriodicalIF":2.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of TRPV4 in ferroptosis in MPP+/MPTP-induced Parkinson’s disease models","authors":"Qingjie Ma ,&nbsp;Jilin Wu ,&nbsp;Huixian Li ,&nbsp;Renwan Yin ,&nbsp;Wenjing Yang ,&nbsp;Jia Luo ,&nbsp;Yuncheng Bai ,&nbsp;Na Liu","doi":"10.1016/j.tice.2025.103019","DOIUrl":"10.1016/j.tice.2025.103019","url":null,"abstract":"<div><h3>Background</h3><div>Transient receptor potential vanilloid 4 (TRPV4) is a nonselective calcium-permeable cation channel. Our previous study revealed that TRPV4 mediates endoplasmic reticulum (ER) stress and inflammation, leading to the loss of dopamine neurons in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson’s disease (PD) model mice.</div></div><div><h3>Objective</h3><div>The aim of the present study was to investigate the molecular role of TRPV4 in ferroptosis in PD models.</div></div><div><h3>Methods</h3><div>We used GSK2193874 (an inhibitor of TRPV4) and siRNA targeting TRPV4 to explore the role of TRPV4 in iron transportation, the production of reactive oxygen species (ROS) and oxidative stress in 1-methyl-4-phenylpyridinium ion (MPP⁺)-treated PC12 cells by H2DCFDA, CCK-8, ELISA and western blot. In a PD mouse model, the intracerebral injection of adeno-associated virus (AAV) was used to knockdown or upregulate TRPV4 expression following the intraperitoneal injection of MPTP and GSK2193874. We used Perl’s iron staining and western blot to detect alterations in iron-positive cells and ferroptosis-associated molecules in the substantia nigra (SN).</div></div><div><h3>Results</h3><div>We found that silencing TRPV4 increased the expression of glutathione peroxidase 4 (GPX4) and ferritin heavy chain (FTH1), decreased divalent metal transporter 1 (DMT1), and alleviated the excessive ROS and oxidative stress in MPP⁺-induced PC12 cells. Moreover, TRPV4 regulated the accumulation of iron in the SN and ferroptosis-associated signalling pathways in PD mice.</div></div><div><h3>Conclusions</h3><div>TRPV4 plays a key role in MPP⁺/MPTP-induced ferroptosis. Our results indicate that the downregulation of TRPV4 may a represent promising treatment for PD through the inhibition of ferroptosis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103019"},"PeriodicalIF":2.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helicobacter pylori infection induces STAT3/MYBL2/NF-κB axis to promote gastric cancer progression","authors":"Zhaotian Feng ,&nbsp;Shuqing Bao ,&nbsp;Wenshuai Zhu ,&nbsp;Yuanxin Xing ,&nbsp;Muhua Luan ,&nbsp;Xiaoli Ma ,&nbsp;Yunshan Wang ,&nbsp;Jingyu Zhu ,&nbsp;Yanfei Jia","doi":"10.1016/j.tice.2025.103016","DOIUrl":"10.1016/j.tice.2025.103016","url":null,"abstract":"<div><div>Infection with <em>Helicobacter pylori</em> (<em>H. pylori</em>) represents a significant risk factor for the development of gastric cancer (GC). The oncogenic functions of MYBL2 have been reported in several malignancies, for which little research has focused on its effect on <em>H. pylori</em>-induced GC progression. We analyzed the expression and clinical relevance of MYBL2 in GC. <em>H. pylori</em> infection was induced in vitro and in vivo. The effects of MYBL2 on cellular functions were assessed, and tumor growth was evaluated in nude mice injected with genetically altered GC cells. We found <em>H. pylori</em> infection significantly increased MYBL2 expression. Downregulation of MYBL2 inhibited <em>H. pylori</em>-induced cell proliferation and migration. MYBL2 contributed to the growth of subcutaneous xenograft tumors. In conclusion, <em>H. pylori</em> infection upregulated MYBL2 expression by activating STAT3, and MYBL2 was found to be an upstream regulator of NF-κB activation in GC cells. In conclusion, our findings indicate that the <em>H. pylori</em>/STAT3/MYBL2/NF-κB axis plays a critical role in the regulation of GC.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103016"},"PeriodicalIF":2.7,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumour necrosis factor-alpha but not Interleukin-1-beta inhibits uniaxial cyclic strain induced tenogenic differentiation of human mesenchymal stromal cells in vitro","authors":"Shaliny Krishnan ,&nbsp;Hui Yin Nam ,&nbsp;Amirah Zulkifli ,&nbsp;Peggy Kong ,&nbsp;Cheh Chin Tai ,&nbsp;Azura Mansor ,&nbsp;Tunku Kamarul","doi":"10.1016/j.tice.2025.103018","DOIUrl":"10.1016/j.tice.2025.103018","url":null,"abstract":"<div><div>Uniaxial cyclic strain induces tenogenic differentiation of mesenchymal stromal cells (MSCs), suggesting its beneficial role in tendon regeneration following injury. However, pro-inflammatory cytokines released during injury is expected to impede repair outcomes but has not been previously considered. A study was thus conducted to determine the effects of interleukin-1-beta (IL-1β) and tumour necrosis factor-alpha (TNF-α) on the tenogenic differentiation potential of cyclic strained MSCs. An <em>in vitro</em> model of MSCs exposed to IL-1β (9 ng/ml) and TNF-α (6 ng/ml) in unstrained and strained conditions, were developed. The effect of these cytokines on MSCs functional properties including proliferation, viability, anabolic/catabolic protein production and tenogenic differentiation potential of MSCs were quantified. IL-1β (9 ng/ml) and TNF-α (6 ng/ml) reduced unstrained MSCs viability, proliferation, ECM protein production and tenogenic markers expression whilst increasing matrix metalloproteinase 13 (MMP-13) expression. Uniaxial strain exposure in IL-1β-treated MSCs reversed the inhibitory effects of this cytokine on cell proliferation, collagen production and tenogenic markers expression. The significant reduction in tenogenic potential observed in MSCs treated with TNF-α was not alleviated by cyclic strain. These data indicate that TNF-α and not IL-1β is able to inhibit tenogenic effects induced by uniaxial cyclic strain on MSCs.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103018"},"PeriodicalIF":2.7,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of L-carnitine over-supplementation on spermatogenesis and sperm function in healthy NMRI mice","authors":"Mitra Jabarineitapeh ,&nbsp;Nushin Naderi ,&nbsp;Marziyeh Tavalaee ,&nbsp;Mohammad Hossein Nasr-Esfahani","doi":"10.1016/j.tice.2025.103014","DOIUrl":"10.1016/j.tice.2025.103014","url":null,"abstract":"<div><div>L-carnitine (LC) plays a key role in lipid metabolism by transporting fatty acids from the cytosol into mitochondria for energy production. Beyond its metabolic function, LC exhibits significant antioxidant, anti-cytokine, and anti-apoptotic properties, contributing to cellular protection. This study investigates the potential adverse effects of four weeks of LC supplementation (20, 100, and 500 mg/kg body weight, n = 10) on spermatogenesis and sperm function in healthy NMRI male mice. Sperm parameters—motility, concentration, and morphology—were assessed alongside sperm and testicular oxidative status. Sperm chromatin and DNA integrity were evaluated through histone persistence (Aniline Blue staining), protamine deficiency (CMA3 staining), and DNA fragmentation (Acridine Orange staining). Pregnancy outcomes were also investigated. After four weeks, the LC500 group exhibited significantly higher liver weight than the controls. However, sperm parameters, reactive oxygen species (ROS) production, lipid peroxidation, and testicular malondialdehyde and total antioxidant capacity levels showed no significant differences among groups. Notably, the LC500 group demonstrated a significant decline in sperm DNA and chromatin integrity (P &lt; 0.05). Alongside, liver sections from LC500-treated mice showed a marked increase in lipid droplets, while controls maintained normal hepatic morphology. Despite these alterations, male fertility indices remained unchanged. Our findings suggest that while LC is essential for metabolism, over-supplementation may compromise male fertility by inducing sperm chromatin damage and DNA fragmentation. Furthermore, high-dose LC intake may contribute to inflammation and non-alcoholic fatty liver disease (NAFLD), emphasizing the need for cautious LC supplementation to safeguard reproductive health.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103014"},"PeriodicalIF":2.7,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a 3D trophoblast model and validation by triiodothyronine (T3) treatment","authors":"Mariana M. Lourenço ,&nbsp;Bianca M. Gonçalves ,&nbsp;Vinícius V. Peghinelli ,&nbsp;Maria T. de Sibio ,&nbsp;Ester M. Vieira ,&nbsp;Helena P. Tilli ,&nbsp;Paula B. Rocha ,&nbsp;Regiane M.C. Olímpio ,&nbsp;Igor C. Deprá ,&nbsp;Lucas S. Mathias ,&nbsp;Marna E. Sakalem ,&nbsp;Carlos R. Padovani ,&nbsp;Célia R. Nogueira","doi":"10.1016/j.tice.2025.103017","DOIUrl":"10.1016/j.tice.2025.103017","url":null,"abstract":"<div><div>During gestation, insufficient triiodothyronine hormone (T3) can lead to failures in trophoblastic activity or induce defects in fetal epigenetic mechanisms. As a consequence, multiple hormones become unbalanced, which can trigger different negative outcomes in the offspring. This study aims to propose the construction of a 3D trophoblast model, validated with different levels of thyroid hormone treatment. Different concentrations of T3, 1, 10 and 100 nM, were employed for 24, 48 and 72 h, corresponding to sub-physiological, physiological and supraphysiological concentrations, respectively, to investigate the effects on human chorionic gonadotropin (hCG), Lactate Dehydrogenase (LDH), and MTT levels in the 3D model. The generated 3D trophoblast model responded differently to the T3 concentrations: although no effect was observed regarding cell viability (MTT assay), there was an increase in cytotoxicity in the group treated with the highest concentration (100 nM; LDH assay) after 24 h. In addition, in the concentration-response curve of hCG, while a natural progressive increase in hCG is observed in control group, there was a clear reduction of hCG in the models treated with 1 nM and 100 nM T3; the 10 nM-treated group demonstrated no difference to control. The proposed 3D trophoblastic placental model was successfully established and validated. Utilizing this model, it was demonstrated that adequate concentrations of T3 are essential for the accurate prediction of cellular function and the prevention of cytotoxic effects. These findings underscore the critical importance of maintaining physiological levels of T3 during pregnancy to support appropriate secretion of hCG.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103017"},"PeriodicalIF":2.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphocreatine alleviates liver fibrosis in diabetic mice by targeting TGF-β/Smad and α-SMA pathways","authors":"Fu Han Wang ,&nbsp;Eskandar Qaed ,&nbsp;Waleed Aldahmash ,&nbsp;Mueataz A. Mahyoub ,&nbsp;Dalal Sanad Al-Mutairi ,&nbsp;Zeyao Tang ,&nbsp;Marwan almoiliqy","doi":"10.1016/j.tice.2025.103013","DOIUrl":"10.1016/j.tice.2025.103013","url":null,"abstract":"<div><h3>Purpose</h3><div>This study aimed to investigate the protective effects of phosphocreatine (PCr) on diabetes-induced liver fibrosis and to explore the underlying mechanisms, particularly its impact on the TGF-β/Smad signaling pathway.</div></div><div><h3>Methods</h3><div>A diabetic liver fibrosis model was established in mice using streptozotocin and carbon tetrachloride (CCl₄). Mice were treated with PCr, and liver tissues were analyzed using histological staining (H&amp;E, Masson), ELISA, Western blot, and high-resolution respirometry (HRR) to assess fibrosis, inflammation, oxidative stress, and mitochondrial function. Protein levels of TGF-β, Smad2/3, α-SMA, and collagen type I were quantified to evaluate HSC activation.</div></div><div><h3>Results</h3><div>PCr significantly reduced collagen deposition and restored liver architecture. It attenuated oxidative stress, as evidenced by lower Malondialdehyde (MDA) levels and higher Superoxide Dismutase SOD activity, reduced pro-inflammatory cytokines including TNF-α and IL-6, improved liver function as shown by decreased ALT and AST levels, and enhanced mitochondrial respiration. Mechanistically, PCr downregulated TGF-β, phosphorylated Smad2/3, α-SMA, and collagen type I, indicating suppression of HSC activation.</div></div><div><h3>Conclusion</h3><div>PCr alleviates liver fibrosis in diabetic mice by inhibiting the TGF-β/Smad pathway and improving mitochondrial function, suggesting its potential as a therapeutic agent for diabetic liver fibrosis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103013"},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteins extracted from placenta regulate osteogenic differentiation of human mesenchymal stem cells","authors":"Benjie Wei ,&nbsp;Ying Chen ,&nbsp;Shengmin Zhang ,&nbsp;Laisen Cui ,&nbsp;Shuo Zhang ,&nbsp;Zhong Zhuang ,&nbsp;Chunhui Sun ,&nbsp;Na Ren ,&nbsp;Hong Liu","doi":"10.1016/j.tice.2025.103015","DOIUrl":"10.1016/j.tice.2025.103015","url":null,"abstract":"<div><div>Placenta is a medical waste, but the human placenta-derived proteins (hPDPs) have highly significant application value. The precise mechanism of injectable hPDPs gel on bone regeneration has been scarcely studied. In this study, a succinct methodology was employed to extract all proteins in the placenta. Proteomic analysis revealed that key gene ontology (GO) terms, signaling pathways, and osteogenic stimulators in PDPs, such as THY1, GDF15, SPARC, GPNMB, PARK7, EFEMP1, and LRP. In order to provide evidence supporting the ability of injectable hPDPs to promote osteogenesis and osteogenic differentiation (OD), the in vitro experiments utilizing human mesenchymal stem cells (hMSCs) and in <em>vivo</em> heterotopic osteogenesis animal models were conducted to assess the bone-forming ability of hPDPs. Results demonstrated that hPDPs accelerated OD of hMSCs and enhanced osteogenesis. Proteomic data, qPCR, immunofluorescence (IF) staining, RNA-sequencing data, KEGG, and GO enrichment analysis were utilized to elucidate the mechanisms underlying hPDP-induced OD. The findings indicated that the PDPs could promote OD through the activation of osteogenic stimulators and multiple signaling pathways, especially the BMP2/TGF-β signaling pathway and the iron metabolism pathway. This research elucidated the function and mechanism of PDPs in OD, providing valuable insights into their potential clinical applications. The findings suggest a novel strategy for utilizing medical waste or their stimulators as biocompatible materials for bone tissue engineering applications.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103015"},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocol for the generation and analysis of organoids from Dental pulp stem cells (DPSCs)","authors":"Loreto Lancia ,&nbsp;Annunziata Mauro ,&nbsp;Vincenzo Mattei ,&nbsp;Simona Delle Monache ,&nbsp;Fanny Pulcini","doi":"10.1016/j.tice.2025.103010","DOIUrl":"10.1016/j.tice.2025.103010","url":null,"abstract":"<div><div>DPSCs are a valuable resource for creating three-dimensional (3D) <em>in vitro</em> models, such as organoids, due to their accessibility and differentiation potential. Organoids have revolutionized <em>in vitro</em> culture, thanks to their ability to more faithfully replicate the architecture and complexity of tissues compared to 2D cultures. This enables their use in a wide range of applications, including research on genetic diseases, innovative therapies, and tissue engineering. This project aims to establish an <em>in vitro</em> protocol for generating 3D models from DPSCs to study dental diseases and test new drugs for regenerative medicine and tissue reconstruction. To this end, the organoids underwent morphological, viability, and functional analyses, allowing us to evaluate the effectiveness of the protocol and the validity of the model. The protocol includes the culture of DPSCs in Matrigel® Matrix, an extracellular matrix that supports 3D-fabricated organoids formation and growth. These 3D structures are exposed to differentiation factors to generate dental pulp organoids. However, the presence of Matrigel® Matrix may interfere with subsequent molecular analyses. To overcome this limitation, an efficient method for removing the Matrigel® Matrix was developed to allow efficient extraction of nucleic acids and proteins. Immunohistochemical and immunofluorescence techniques were also optimized to visualize cellular structures and markers. Cell count and proliferation were assessed using a CCK-8 viability assay, as well as Trypan Blue staining. The feasibility and effectiveness of this protocol can provide a new tool for studying dental pulp biology, paving the way for future applications in the field of dental pulp regeneration research.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103010"},"PeriodicalIF":2.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}