Tissue & cell最新文献

{"title":"Spermatophore formation in Todarodes pacificus (Cephalopoda: Ommastrephidae).","authors":"So Ryung Shin, Jung Jun Park, Jung Sick Lee","doi":"10.1016/j.tice.2026.103985","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103985","url":null,"abstract":"<p><p>This study elucidated the ultrastructure of spermatophore formation in Todarodes pacificus. The spermatophore of T. pacificus is a slender, elongated, club-shaped structure composed of an outer tunic, middle tunic, inner tunic, sperm mass, cement body, ejaculatory apparatus, and cap thread. Spermatophore formation occurred sequentially in the vas deferens, spermatophoric gland, and spermatophoric sac. In the vas deferens, sperm were aggregated and organized to form the sperm mass, and distinct differences in sperm arrangement were observed between the anterior and posterior regions. In the spermatophoric gland, the major components of the spermatophore-including the tunics, cement body, and ejaculatory apparatus-were formed. The epithelial layer of the spermatophoric gland consisted of epithelial and secretory cells containing fibrous and granular secretory materials. These secretory cells were classified into five types (Types A-E) according to their histochemical characteristics, and each type was associated with the formation of specific spermatophore components. The formed spermatophores were subsequently stored in the spermatophoric sac. This study provides a detailed microanatomical description of spermatophore formation in T. pacificus and offers fundamental insights into cephalopod reproductive physiology.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"104 Pt 2","pages":"103985"},"PeriodicalIF":3.1,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory effect of surface functionalized medical zinc alloy on the growth and inflammatory response of vascular endothelial cells.","authors":"Lijun Liu, Xiaoli Zhang, Yan Hu, Weimin Li","doi":"10.1016/j.tice.2026.103990","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103990","url":null,"abstract":"<p><strong>Objective: </strong>The growth of endothelial cells and their resistance to inflammation seriously affect the repair and regeneration of blood vessels, the integrity of vascular structure, and the cellular immune response function. At present, many materials are capable of regulating the growth and inflammatory response of vascular endothelial cells. However, their regulatory effects are not ideal and still need to be optimized.</p><p><strong>Methods: </strong>This study utilizes layer by layer assembly technology to assemble Chitosan (CS) with Zn-0.8Li alloy, functionalized the surface of the alloy, and forms a CS thin film on the surface of Zn-0.8Li to synthesize CS-Zn-0.8Li material. Moreover, human umbilical vein endothelial cells are used as experimental subjects. CS-Zn-0.8Li and the extraction solution of the control material are added to the cell culture medium, and after 24 h of cultivation, the cell activity and proliferation ability are detected by MTT assay. The levels of pro-inflammatory and anti-inflammatory factors such as TNF - α and IL-6 are measured using an inflammatory factor detection kit, and the expression levels of inflammatory response signaling pathway molecules are analyzed.</p><p><strong>Results: </strong>At a concentration of 20% in the leaching solution, the activity of cells can reach 91.2%, which is higher than the 83.7% of Zn-0.8Li. In addition, the proliferation ability of cells in the new material can reached 66.7%, which is 6.9% higher than that of Zn-0.8Li. The regulatory effect of the new material on inflammatory response is analyzed. It is found that after 24 h of cultivation, the content of anti-inflammatory factors in the 20% concentration of the new material culture dish is greater than 75%, and the content of pro-inflammatory factors is less than 16%, indicating a relatively low content. In both Zn-0.8Li and the new material culture dishes, the expression level of inflammatory signaling pathway molecules in the new material culture dish is less than 13%.</p><p><strong>Conclusion: </strong>The above results indicate that the proposed Zn-0.8Li alloy fused with CS can promote cell proliferation ability and inhibit inflammation related factors, thereby regulating the growth of vascular endothelial cells and inflammatory response.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"104 Pt 2","pages":"103990"},"PeriodicalIF":3.1,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum \"A novel immortalization method for immortalizing human primary CD8⁺ T cells by inserting a single copy of human telomerase reverse transcriptase via CRISPR/Cas9\"[Tissue and Cell 95 (2025), 102908].","authors":"Zhiyong He, Kenneth D Cole, Hua-Jun He","doi":"10.1016/j.tice.2026.103977","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103977","url":null,"abstract":"","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":" ","pages":"103977"},"PeriodicalIF":3.1,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149008947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Enhanced renoprotective effects of remote ischemic postconditioning and alpha-lipoic acid via modulation of TLR4/MyD88/NF-κB and Nrf2/HO-1 pathways in renal ischemia-reperfusion injury\" [Tissue Cell 104(2027) 103897].","authors":"Murtadha Ghafil Jasim Hamdan, Firouzeh Gholampour, Zeinab Karimi, Gholamreza Daryabor","doi":"10.1016/j.tice.2026.103947","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103947","url":null,"abstract":"","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":" ","pages":"103947"},"PeriodicalIF":3.1,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148920973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphocreatine mitigates pulmonary fibrosis in STZ-induced diabetic rats with bleomycin injury through TGF-β/Smad and Wnt/β-catenin pathways.","authors":"Fu Han Wang, Abdulrahman Khaled Alwesabi, Eskandar Qaed, Waleed Aldahmash, Mueataz A Mahyoub, Marwan Almoiliqy","doi":"10.1016/j.tice.2026.103875","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103875","url":null,"abstract":"<p><p>Diabetes accelerates pulmonary fibrosis through chronic inflammation, oxidative stress, and fibroblast activation. Key signaling pathways, including TGF-β/Smad and Wnt/β-catenin, drive fibroblast activation and epithelial-mesenchymal transition (EMT), contributing to lung fibrotic remodeling. This study investigated the therapeutic potential of phosphocreatine (PCr) in a combined model of STZ-induced diabetes and bleomycin-induced lung fibrosis. Histopathological changes were assessed using H&E, Masson's Trichrome, and Sirius Red staining, revealing alveolar injury, inflammatory infiltration, and collagen deposition. Mitochondrial function was evaluated by measuring ATP production and citrate synthase activity. PCr treatment markedly mitigated lung fibrosis, preserved alveolar structure, restored mitochondrial bioenergetics, and reduced inflammation and oxidative stress, likely via modulation of TGF-β/Smad and Wnt/β-catenin signaling. These findings suggest that PCr may serve as a promising multi-targeted therapeutic strategy for diabetes-associated pulmonary fibrosis.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"104 Pt 1","pages":"103875"},"PeriodicalIF":3.1,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of histone deacetylases accelerates wound healing in diabetic skin and is associated with vascularization and epidermal tight junction protein expression.","authors":"Juan Valentin Trujillo-Paez, Adrián Rodríguez-Carlos, Nancy Solís-Torres, Yessica E Félix-García, Irma González-Curiel, Luis Adrián De Jesús-González, Crisol Rodriguez Mendieta, César Rivas-Santiago, Bruno Rivas-Santiago","doi":"10.1016/j.tice.2026.103868","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103868","url":null,"abstract":"<p><strong>Background: </strong>Chronic wounds in diabetic patients, represent a significant clinical challenge due to impaired epidermal barrier function and deficient wound healing. The development of novel therapeutic strategies aimed at enhancing both barrier integrity and wound healing is therefore critical. Recent evidence suggests that histone deacetylase inhibitors (iHDACs) may modulate wound healing.</p><p><strong>Objective: </strong>This study aimed to evaluate the effect of iHDACs, 1,3-Diphenylurea (DiPU), 2'-Aminoacetanilide (Ace), and Tert-butyl (2-aminophenyl) carbamate (N-boc), on epidermal barrier integrity and tight junction (TJ) protein expression, as well as their ability to promote wound healing and vascularization under diabetes conditions.</p><p><strong>Methods: </strong>Diabetes was induced in mice by streptozotocin (STZ) administration. Full-thickness excisional wounds were created, and wound closure was quantified by digital planimetry. Tissue samples were analyzed by H&E and Masson's trichrome staining to assess histological changes and collagen deposition. claudin-1, ZO-1, and occludin were evaluated by immunohistochemistry. Vascularization was quantified by measuring microvessel density.</p><p><strong>Results: </strong>Treatment with iHDACs improved re-epithelialization and increased the expression of tight junction proteins, suggesting restoration of molecular components associated with epidermal barrier integrity. Moreover, all treatments promoted wound closure. Macroscopic observations and quantitative analyses further demonstrated that N-boc and Ace significantly enhanced the formation of denser and more organized vascular networks in both healthy and diabetic mice, whereas DiPU produced a more moderate pro-angiogenic response in diabetic wounds.</p><p><strong>Conclusion: </strong>These findings indicate that iHDACs may offer therapeutic benefit by reinforcing epidermal barrier function and stimulating wound healing and vascularization, supporting their potential use as treatments for chronic wounds.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"104 Pt 1","pages":"103868"},"PeriodicalIF":3.1,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zhuhuang granules inhibit HIF-1α signaling to regulate oxidative stress-mitochondrial function axis to improve psoriasis.","authors":"Hui Shen, Qiuyu Bao, Wenxin Luo, Xiaolei Yao","doi":"10.1016/j.tice.2026.103861","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103861","url":null,"abstract":"<p><p>Psoriasis is a chronic immune-mediated skin disorder characterized by keratinocyte hyperproliferation and persistent inflammation. This study evaluated the therapeutic effects of Zhuhuang granules and investigated their underlying mechanism. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), network pharmacology, and molecular docking were combined with an imiquimod (IMQ)-induced psoriasis-like mouse model and a HaCaT cell model stimulated with an M5 cytokine cocktail (interleukin-17A, interleukin-22, oncostatin M, interleukin-1α, and tumor necrosis factor-α). UPLC-MS/MS identified multiple compounds and 174 absorbed prototype components. Network analysis prioritized the hypoxia-inducible factor 1 subunit alpha gene (HIF1A), while molecular docking predicted potential interactions of gardenoside and kaempferol with the encoded hypoxia-inducible factor-1α (HIF-1α) protein. In the psoriasis mouse model, Zhuhuang granules dose-dependently alleviated erythema, scaling, and epidermal thickening, with methotrexate serving as a positive control. Zhuhuang granules also reduced reactive oxygen species (ROS), malondialdehyde, interleukin-17A, and interleukin-23 levels, restored glutathione levels and superoxide dismutase and catalase activities, and preserved mitochondrial membrane potential. In M5-stimulated HaCaT cells, Zhuhuang granule-containing serum inhibited proliferation and inflammatory cytokine release, reduced mitochondrial ROS, lactate, and mitochondrial DNA release, and improved mitochondrial membrane potential. These effects were accompanied by reduced HIF-1α and tumor necrosis factor receptor 1 protein levels and phosphoinositide 3-kinase phosphorylation. Molidustat attenuated these protective effects. Collectively, Zhuhuang granules ameliorate psoriasis-like alterations by suppressing HIF-1α-associated oxidative stress, inflammation, and mitochondrial dysfunction.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"104 Pt 1","pages":"103861"},"PeriodicalIF":3.1,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroimmune pharmacology of infliximab and lycopene in repetitive traumatic brain injury: Targeting SIRT1/miR-21-mediated brain-lung crosstalk.","authors":"Hoda E Mohamed, Amira A El-Gazar, Rana M Hegazy, Gehad M Elnagar","doi":"10.1016/j.tice.2026.103864","DOIUrl":"https://doi.org/10.1016/j.tice.2026.103864","url":null,"abstract":"<p><p>Traumatic brain injury (TBI) is a critical reason of neurological impairment globally, with increasing evidence suggesting that its impact extends beyond the brain to peripheral organs. This study investigates the potential brain-lung crosstalk in mild repetitive TBI (RTBI) and explores the therapeutic potential of Infliximab (INF) and Lycopene (LYC) in mitigating the associated neuroinflammatory and pulmonary complications. Using a weight-drop rat model of mild RTBI, we tracked the histological changes and inflammatory markers in both brain and lung tissues across control, RTBI, and treatment groups with INF, LYC, and their combination. Our findings revealed that RTBI induced significant neuroinflammation and oxidative stress, characterized by sirtuin 1 (SIRT1) suppression, activation of NLRP3 (Nod-like receptor protein containing pyrin 3), apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) and caspase-1 inflammasome, as well as elevated brain levels of tumor necrosis factor alpha (TNF-α) and nuclear factor kappa B (NF-ҡB). These changes were associated with the increased pulmonary micro-ribonucleic acid-21 (micRNA-21) expression, pulmonary inflammasome activation, and pyroptosis-associated damage, suggesting systemic inflammation and oxidative stress. However, the post-treatment with INF and LYC significantly reduced neuroinflammation, partially restored SIRT1 levels, and inflammasome activity. Therefore, these effects may contribute to the restoration of systemic homeostasis and attenuation of lung injury markers. Notably, the combination therapy showed superior protective effects compared with individual treatments. These results suggest an interconnected pathology of RTBI and lung injury, emphasizing how oxidative stress and systemic inflammation contribute to secondary organ damage. The anti-inflammatory and neuroprotective effects of INF and LYC highlight their potential as therapeutic agents for reducing multi-organ complications in RTBI. Further investigations are required to explore their translational applications in clinical settings and establish mechanistic and inter-organ relationships.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"104 Pt 1","pages":"103864"},"PeriodicalIF":3.1,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Shoeblackplant extract-loaded carboxymethyl cellulose/collagen scaffolds seeded with adipose-derived stem cells enhance diabetic wound healing via immunomodulation and angiogenic pathways\" [Tissue Cell 100 (2026) 103393].","authors":"Xiaoling Wang, Haiying Yang, Jie Hu, Saman Jalili","doi":"10.1016/j.tice.2026.103434","DOIUrl":"10.1016/j.tice.2026.103434","url":null,"abstract":"","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":" ","pages":"103434"},"PeriodicalIF":2.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147370430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chrysin as a therapeutic adjunct to prevent isoniazid-induced nephrotoxicity in rats: Biochemical, molecular, and histopathological evidence.","authors":"Özge Kandemir, Fatih Mehmet Kandemir, Sefa Küçükler, Selim Çomaklı, Cuneyt Caglayan, Elif Dalkılınç","doi":"10.1016/j.tice.2026.103527","DOIUrl":"10.1016/j.tice.2026.103527","url":null,"abstract":"<p><p>Isoniazid (INZ) is an effective antituberculosis drug; however, its use is associated with nephrotoxicity due to the induction of oxidative stress, inflammation, and apoptosis. This study investigated whether chrysin (CHR), a natural flavonoid with antioxidant and anti-inflammatory properties, could protect against INZ-induced nephrotoxicity in rats. Male Sprague Dawley rats were divided into five groups: Control, INZ, CHR, INZ+CHR25 and INZ+CHR50. Biochemical assays, ELISA, RT-qPCR, Western blot and immunohistochemistry were used to evaluate renal function markers, oxidative stress parameters, inflammatory mediators, survival genes, apoptosis, autophagy and endoplasmic reticulum (ER) stress-associated genes. INZ treatment was found to significantly increase serum urea and creatinine levels, cause glomerular and tubular damage, raise MDA levels, lower antioxidant enzymes (SOD, CAT and GPx) and GSH levels, and suppress Nrf-2, HO-1, SIRT1 and PGC1α. Furthermore, INZ treatment was found to upregulate inflammatory markers (NFκB, STAT3, TNF-α, IL-1β, MAPK and JNK), apoptotic markers (Bax, Bcl2, P53, and P62), components of the PI3K/AKT/mTOR pathway and ER stress/autophagy genes (GRP78, ATF6, PERK, IRE-1α, CHOP and Beclin-1). Co-administration of CHR reversed these alterations in a dose-dependent manner, enhancing antioxidant defenses, attenuating inflammation, apoptosis, autophagy, and ER stress. CHR also reduced kidney injury by increasing nephrin expression and decreasing KIM-1 expression immunohistochemically. These findings suggest that CHR has a nephroprotective effect against INZ-induced renal injury through multi-target molecular mechanisms and demonstrate its potential as an adjunctive therapy to reduce drug-induced nephrotoxicity.</p>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"101 ","pages":"103527"},"PeriodicalIF":3.1,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}