聚己内酯支架中负载镁的碳量子点促进血管生成和加速伤口愈合：体外，卵内和体内证据。

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-25 DOI:10.1016/j.tice.2025.103158

Tannaz Afshin , Saber Zahri , Arash Abdolmaleki , Asadolah Asadi , Habibolah Eskandari

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引用次数: 0

摘要

伤口愈合和组织再生是关键挑战，特别是在糖尿病等慢性疾病中，血管生成受损往往会延迟恢复。本研究通过合成镁负载碳量子点（Mg-CQDs）并将其植入聚己内酯（PCL）电纺丝支架中，开发了一种新型生物活性伤口敷料。采用水热法制备CQDs，并通过离子相互作用与Mg 2 +结合，形成具有增强荧光和生物活性的纳米复合材料。表征证实了它们的结构完整性和表面改性。使用PC12细胞进行的体外划痕实验显示，Mg-CQD处理显著改善了细胞迁移。卵内绒毛膜尿囊膜（CAM）检测显示新生血管增强，大鼠体内伤口愈合研究显示，与对照组相比，mg - cqd处理组组织再生加速，上皮化增加，胶原沉积增加。与对照组相比，CQD组的VLDM增强幅度为0.611 %至0.749 %，CQD- mg组的VLDM增强幅度为0.802 %至1.19 %。这些结果表明，mg - cqd负载的PCL支架提供了一个促进血管生成和伤口修复的多功能平台，将结构支持与靶向离子传递相结合，具有潜在的治疗应用前景。

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Magnesium-loaded carbon quantum dots in polycaprolactone scaffolds enhance angiogenesis and accelerate wound healing: In vitro, In Ovo, and in vivo evidence

Wound healing and tissue regeneration are critical challenges, particularly in chronic conditions such as diabetes, where impaired angiogenesis often delays recovery. In this study, a novel bioactive wound dressing was developed by synthesizing magnesium-loaded carbon quantum dots (Mg-CQDs) and incorporating them into polycaprolactone (PCL) electrospun scaffolds. CQDs were prepared hydrothermally and subsequently bonded with Mg²⁺ ions via ionic interaction, forming nanocomposites with enhanced fluorescence and bioactivity. Characterization confirmed their structural integrity and surface modification. In vitro scratch assays using PC12 cells demonstrated significantly improved cell migration with Mg-CQD treatment. In ovo chorioallantoic membrane (CAM) assays showed enhanced neovascularization, and in vivo wound healing studies in rats revealed accelerated tissue regeneration, increased epithelialization, and collagen deposition in Mg-CQD-treated groups compared to controls. Compared to the reference group, VLDM enhancements ranged from 0.611 % to 0.749 % for the CQD group and from 0.802 % to 1.19 % for the CQD-Mg group. These results suggest that Mg-CQD-loaded PCL scaffolds offer a multifunctional platform for promoting angiogenesis and wound repair, combining structural support with targeted ion delivery and potential theranostic applications.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.