Naringenin mitigates 5-fluorouracil-induced nephrotoxicity by decreasing oxidative stress, inflammation and apoptosis

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-29 DOI:10.1016/j.tice.2025.103170

Fatih Mehmet Gür , Sedat Bilgiç , İbrahim Aktaş

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引用次数: 0

Abstract

The toxic effects of 5-fluorouracil (5-FU), a drug widely used in the treatment of neoplasms and primarily excreted through the urine, prompted us to investigate the protective potential of naringenin (NAR) against nephrotoxicity induced by 5-FU. In this study, 28 rats were randomly allocated to four groups: Control, NAR (100 mg/kg/day), 5-FU (150 mg/kg), and NAR + 5-FU (n = 7). After completion of the experimental procedures, blood and kidney tissues were subjected to analysis through biochemical, immunohistochemical, TUNEL, and histopathological methods. The analyses demonstrated significantly higher levels of MDA, BUN, TNF-α, caspase-3, and apoptosis in the 5-FU group compared to the control. Pathological alterations, including inflammatory cell infiltration, interstitial hemorrhage, brush border damage, epithelial desquamation, vacuolar degeneration, and basement membrane thickening, observed in the 5-FU group were largely absent in the NAR +5-FU group. In conclusion, NAR can significantly prevent the nephrotoxic effects of 5-FU due to its antioxidant, anti-inflammatory, and anti-apoptotic properties. The findings support the view that the therapeutic effects of NAR are mediated through the upregulation of Nrf2 and the downregulation of NF-κB.

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柚皮素通过降低氧化应激、炎症和细胞凋亡来减轻5-氟尿嘧啶引起的肾毒性。

5-氟尿嘧啶（5-FU）是一种广泛用于肿瘤治疗的药物，主要通过尿液排出，其毒性作用促使我们研究柚皮素（NAR）对5-FU引起的肾毒性的保护潜力。本研究将28只大鼠随机分为4组：对照组、NAR（100 mg/kg/day）、5-FU（150 mg/kg）和NAR + 5-FU （n = 7）。实验程序完成后，通过生化、免疫组织化学、TUNEL和组织病理学方法对血液和肾脏组织进行分析。分析显示，与对照组相比，5-FU组的MDA、BUN、TNF-α、caspase-3和凋亡水平显著升高。病理改变，包括炎症细胞浸润、间质出血、刷状边界损伤、上皮脱屑、空泡变性和基底膜增厚，在5-FU组中观察到，在NAR +5-FU组中基本没有。综上所述，NAR可通过其抗氧化、抗炎和抗凋亡的特性，显著阻止5-FU的肾毒性作用。研究结果支持了NAR的治疗作用是通过上调Nrf2和下调NF-κB介导的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.