Resveratrol-pretreated ADSC-CM alleviates liver oxidative damage in mice by mediating the Sirt1/Nrf2 axis

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-08-22 DOI:10.1016/j.tice.2025.103099

Jingwen Feng , Chenhao Chang , Ziqiang Zhang , Leyao Hou, Yanni Zhong, Tianao Zhang, Qiongxia Lv, Dongliu Luo, Yumei Liu

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引用次数: 0

Abstract

Chronic liver injury is a prevalent pathological condition. Adipose-derived stem cell-conditioned medium (CM) has demonstrated efficacy in attenuating liver injury. Resveratrol (Res) pretreatment enhances the therapeutic effect of ADSCs, but whether it can enhance the protective effect of CM against CCl₄-induced liver injury is unknown. Therefore, we investigated the effects of CM and Res-pretreated adipose-derived stem cell-conditioned medium (R-CM) on liver structure, fibrosis, proliferation, oxidative stress, and mitochondrial pathway apoptosis in CCl₄-injured mice and further explored the underlying regulatory mechanism of the antioxidant effects of Res on CM. These findings indicated that R-CM was more effective than CM in mitigating CCl₄-induced liver injury, fibrosis, oxidative stress, and apoptosis. Moreover, we found that Res enhances the antioxidant capacity of CM by mediating the Sirt1/Nrf2 axis. Overall, our study demonstrated that CM derived from ADSCs could alleviate CCl₄-induced hepatic oxidative stress, and we found that Res could increase the antioxidant capacity of CM, which is closely related to the regulation of the Sirt1/Nrf2 pathway.

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白藜芦醇预处理的ADSC-CM通过介导Sirt1/Nrf2轴减轻小鼠肝脏氧化损伤

慢性肝损伤是一种常见的病理状况。脂肪来源的干细胞条件培养基（CM）已被证明具有减轻肝损伤的功效。白藜芦醇（Resveratrol, Res）预处理可增强ADSCs的治疗效果，但是否能增强CM对ccl4诱导的肝损伤的保护作用尚不清楚。因此，我们研究了CM和Res预处理的脂肪源性干细胞条件培养基（R-CM）对ccl4损伤小鼠肝脏结构、纤维化、增殖、氧化应激和线粒体通路凋亡的影响，并进一步探讨Res对CM抗氧化作用的潜在调控机制。这些结果表明，R-CM在减轻ccl4诱导的肝损伤、纤维化、氧化应激和细胞凋亡方面比CM更有效。此外，我们发现Res通过介导Sirt1/Nrf2轴增强CM的抗氧化能力。综上所述，我们的研究表明来自ADSCs的CM能够缓解ccl4诱导的肝脏氧化应激，我们发现Res能够提高CM的抗氧化能力，这与Sirt1/Nrf2通路的调控密切相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.