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Study on the role and function of RRS1 in cutaneous squamous cell carcinoma RRS1在皮肤鳞状细胞癌中的作用及功能研究
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-05-01 DOI: 10.1016/j.tice.2025.102949
Zhan-Yan Pan , Zhi-Nan Shi , Qiong Wu , Ting-Ting Hu , Xiao-Hui Mo , Da-Ke Dong , Qiang Ju
{"title":"Study on the role and function of RRS1 in cutaneous squamous cell carcinoma","authors":"Zhan-Yan Pan ,&nbsp;Zhi-Nan Shi ,&nbsp;Qiong Wu ,&nbsp;Ting-Ting Hu ,&nbsp;Xiao-Hui Mo ,&nbsp;Da-Ke Dong ,&nbsp;Qiang Ju","doi":"10.1016/j.tice.2025.102949","DOIUrl":"10.1016/j.tice.2025.102949","url":null,"abstract":"<div><div>RRS1 (regulator of ribosome synthesis 1) has been identified as a critical factor in the proliferation, metastasis, and invasion of some cancer’s cells. In this study, immunohistochemical analysis and GEO database studies demonstrated significantly higher RRS1 expression in cutaneous squamous cell carcinoma(cSCC) tissues and neck squamous cell carcinoma (HNSCC) compared to normal skin. To further investigate RRS1's role, a lentiviral transduction method was used to establish an RRS1 knockdown model in A431 cells. Following RRS1 knockdown, MTT, Celigo scratch, and Transwell assays revealed a significant reduction in A431 cell proliferation, metastasis, and invasiveness. Gene expression analysis by qRT-PCR identified notable changes in genes related to these cellular processes, while transcriptome sequencing highlighted five key genes—protein kinase C delta(PRKCD), mitogen-activated protein kinase 3(MAPK3), transient receptor potential cation channel subfamily V member 4(TRPV4), ankyrin 1(ANK1), and phospholipase A2 group VI(PLA2G6)—showing significantly reduced expression after RRS1 knockdown. These findings suggest that RRS1 plays a crucial role in promoting cSCC tumor cell growth and dissemination and highlight its potential as a novel therapeutic target.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102949"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Red and Red/NIR OLEDs photobiomodulation effects towards promoting ADMSCs chondrogenic differentiation 红色和红/近红外oled光生物调节作用对促进ADMSCs软骨分化的影响
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-05-01 DOI: 10.1016/j.tice.2025.102948
Yeon Tae Kang , Tran Thien Tri , Deok Su Jo , Karthika Muthuramalingam , Hyun Jong Lee
{"title":"Impact of Red and Red/NIR OLEDs photobiomodulation effects towards promoting ADMSCs chondrogenic differentiation","authors":"Yeon Tae Kang ,&nbsp;Tran Thien Tri ,&nbsp;Deok Su Jo ,&nbsp;Karthika Muthuramalingam ,&nbsp;Hyun Jong Lee","doi":"10.1016/j.tice.2025.102948","DOIUrl":"10.1016/j.tice.2025.102948","url":null,"abstract":"<div><div>Photobiomodulation (PBM) uses light to stimulate cellular responses and has the potential to enhance cartilage regeneration through mesenchymal stem cell (MSC) differentiation. This study investigates the chondrogenic potential of adipose-derived MSCs (ADMSCs) under PBM using Red (630 nm, 72.7 mW) and Red/NIR (630 nm and 845 nm, 59.7 mW) organic light-emitting diodes (OLEDs), with ADMSCs exposed to two distinct OLED panels (10.85 cm<sup>2</sup> surface area each). These wavelengths correspond to the absorption peaks of cytochrome c oxidase (CCO), a key mitochondrial enzyme involved in PBM-induced cellular bioenergetics. Cells were cultured in 24-well cell culture plate, receiving irradiance of 3.6 mW/cm<sup>2</sup> and 2.74 mW/cm<sup>2</sup>, respectively. Exposure durations were 4.5, 14, and 23 minutes for Red OLED and 6, 18, and 30 minutes for Red/NIR OLED, delivering energy doses of 1, 3, and 5 J/cm² at 72-hour intervals. While Red/NIR OLED irradiation significantly enhanced ADMSC proliferation, it did not improve chondrogenic differentiation. In contrast, Red OLEDs consistently outperformed Red/NIR OLEDs as evidenced by stronger Safranin O staining, elevated collagen type II expression, and enhanced glycosaminoglycan (GAG) deposition via Alcian Blue staining. These findings underscore the superior efficacy of Red OLED-mediated PBM in promoting ADMSC differentiation toward the chondrogenic lineage and highlight the critical role of wavelength selection for PBM-based cartilage repair. Further exploration of the underlying mechanisms and optimization of PBM parameters is needed for improved clinical efficacies in tissue engineering and regenerative medicine.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102948"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TACC3 facilitates chondrocyte differentiation by attenuating abnormally activated FGFR3 signaling in achondroplasia − An in vitro study TACC3通过减弱软骨发育不全中异常激活的FGFR3信号通路,促进软骨细胞分化
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-04-30 DOI: 10.1016/j.tice.2025.102940
Xiang Li , Long Bao , Xiaoyan Wang , Haiying Wu , Ting Chen , Rongrong Xie , Hui Sun , Dandan Zhang , Lili Wang , Linqi Chen
{"title":"TACC3 facilitates chondrocyte differentiation by attenuating abnormally activated FGFR3 signaling in achondroplasia − An in vitro study","authors":"Xiang Li ,&nbsp;Long Bao ,&nbsp;Xiaoyan Wang ,&nbsp;Haiying Wu ,&nbsp;Ting Chen ,&nbsp;Rongrong Xie ,&nbsp;Hui Sun ,&nbsp;Dandan Zhang ,&nbsp;Lili Wang ,&nbsp;Linqi Chen","doi":"10.1016/j.tice.2025.102940","DOIUrl":"10.1016/j.tice.2025.102940","url":null,"abstract":"<div><h3>Background</h3><div>Achondroplasia is a common form of dwarfism. It is caused by mutations in the fibroblast growth factor receptor 3 (FGFR3), which inhibits chondrocyte proliferation and differentiation.</div></div><div><h3>Aim</h3><div>In this study, we intended to investigate the underlying mechanism of FGFR3 mutation-induced chondrocyte differentiation defection.</div></div><div><h3>Method</h3><div>Insulin-transferrin-selenium (ITS-G) stimulated ATDC5 cells was used as an <em>in vitro</em> model. Alcian Blue staining was performed to detect ATDC5 cell differentiation.</div></div><div><h3>Results</h3><div>TACC3 expression was increased during ATDC5 cell differentiation. ITS-G induced ATDC5 cell differentiation was inhibited by the FGFR3 mutation, as evidenced by the decreased expression of ACAN and COL2A1. The downregulation of TACC3 expression induced by ITS-G stimulation was upregulated by FGFR3 overactivation. The TACC3 inhibitor (KHS101) promoted differentiation in FGFR3 mutant ATDC5 cells. The p38 signaling pathway has been implicated in FGFR3 mutation-induced chondrocyte differentiation defects. KHS101 promoted the expression of p38. KHS101-induced increase in ATDC5 cell differentiation was inhibited by the administration of a p38 inhibitor. These results suggest that TACC3 might play a role through the p38 signaling pathway in chondrocyte differentiation defects caused by FGFR3 mutations.</div></div><div><h3>Conclusion</h3><div>TACC3 might represent a novel target for achondroplasia.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102940"},"PeriodicalIF":2.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxygen-glucose deprivation induces actin spillover in brain endothelial cells 氧葡萄糖剥夺诱导脑内皮细胞肌动蛋白溢出
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-04-29 DOI: 10.1016/j.tice.2025.102946
Yiyin Zhao , Xiaojing Zhou , Songbin He , Jingjing Liu , Meng Jin , Jiaqian Li , Lulan Pan , Lin Zhou
{"title":"Oxygen-glucose deprivation induces actin spillover in brain endothelial cells","authors":"Yiyin Zhao ,&nbsp;Xiaojing Zhou ,&nbsp;Songbin He ,&nbsp;Jingjing Liu ,&nbsp;Meng Jin ,&nbsp;Jiaqian Li ,&nbsp;Lulan Pan ,&nbsp;Lin Zhou","doi":"10.1016/j.tice.2025.102946","DOIUrl":"10.1016/j.tice.2025.102946","url":null,"abstract":"<div><div>Stroke is the leading cause of death and disability worldwide, and the mechanisms of stroke onset have not been fully elucidated. The research investigated how actin remodeling functions within brain microvascular endothelial cells (bEnd.3 cell<span><span><sup>2</sup></span></span>) when exposed to glucose-oxygen deprivation (OGD<span><span><sup>3</sup></span></span>) circumstances. OGD exposure for 6 h in bEnd.3 cell led to increased F-actin polymerization and actin overflow into the supernatant which demonstrated a disruption of intracellular actin balance. This process is mainly mediated by the cofilin and myosin light chain (MLC<span><span><sup>4</sup></span></span>) phosphorylation. Jasplakinolide further enhanced F-actin polymerization, while Latrunculin B inhibited actin polymerization and alleviated cellular damage. In conclusion, our research has revealed the crucial role of actin overflow driven by cofilin and MLC signals in brain endothelial cell injury, providing new insights into the pathophysiology of stroke.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102946"},"PeriodicalIF":2.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing TB diagnosis: Improving specificity with scFv antibodies targeting the PPE17 epitope 增强结核病诊断:提高靶向PPE17表位的scFv抗体的特异性
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-04-29 DOI: 10.1016/j.tice.2025.102933
Kamran Heidarnejad , Mehrzad Bahtouee , Seyed Nooreddin Faraji , Setareh Moazen , Farhad Abbasi , Amirhossein Sahebkar , Foroogh Nejatollahi
{"title":"Enhancing TB diagnosis: Improving specificity with scFv antibodies targeting the PPE17 epitope","authors":"Kamran Heidarnejad ,&nbsp;Mehrzad Bahtouee ,&nbsp;Seyed Nooreddin Faraji ,&nbsp;Setareh Moazen ,&nbsp;Farhad Abbasi ,&nbsp;Amirhossein Sahebkar ,&nbsp;Foroogh Nejatollahi","doi":"10.1016/j.tice.2025.102933","DOIUrl":"10.1016/j.tice.2025.102933","url":null,"abstract":"<div><h3>Background</h3><div>Serological assays have demonstrated enhanced simplicity, accuracy, and effectiveness in detecting Mycobacterium Tuberculosis (Mtb) antigens. The proline-proline-glutamic acid 17 (PPE17) antigen, specifically localized on the surface of Mtb, has been identified as unique to the Mtb species. The unique properties of single-chain antibodies make them well-suited for accurate diagnostic applications. In this study, specialized single-chain antibodies (scFvs) targeting PPE17 were employed to create a precise indirect immunofluorescent assay for diagnosing pulmonary tuberculosis (TB).</div></div><div><h3>Methods</h3><div>To select an immunodominant epitope of PPE17 in silico analysis was applied. The sequence was evaluated using the BLAST algorithm. A phage antibody display library of scFv was applied and two scFvs were isolated against the epitope by panning process. Specific clones were distinguished through PCR and DNA fingerprinting techniques. The reactivity of the chosen scFvs towards the selected epitope was assessed by ELISA. An Indirect Immunofluorescence Assay (IFA) was performed on 50 positive and 50 negative TB sputum smears, which were confirmed through both culture and genotype methods, to evaluate the performance of anti-PPE17 scFvs in accurately and rapidly detecting TB-positive smears, and TB-negative and Nocardia smears serving as negative controls for comparison.</div></div><div><h3>Results</h3><div>An immunodominant epitope of the PPE17 antigen consisting of amino acids 27–39, was identified. Two specific anti-PPE17-scFvs with frequencies of 25 % and 20 % were selected. ELISA results confirmed the reactivity of the scFvs against the epitope. Immunofluorescence assays demonstrated positive results for both antibodies when tested against positive TB sputum smears, whereas no positive results were obtained in tests against TB-negative and Nocardia smears.</div></div><div><h3>Conclusion</h3><div>A fast and accurate indirect immunofluorescence assay was developed to identify Mtb bacteria in TB sputum smears using specific anti-PPE17 scFvs. The results illustrated the capability of both scFvs in detecting Mtb in TB samples and differentiating Mtb from Nocardia smears. This suggests the potential for a novel diagnostic test that ensures precise TB detection in sputum samples, thereby preventing any potential misdiagnosis of tuberculosis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102933"},"PeriodicalIF":2.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tetracera asiatica flavonoids attenuate alcohol-induced liver injury by suppressing oxidative stress and inflammation mediated by the Keap-1/Nrf2/HO-1, NF-κB/MAPK and PERK/Nrf2 signaling pathways in alcoholic liver injury rats 黄酮类化合物通过抑制酒精性肝损伤大鼠Keap-1/Nrf2/HO-1、NF-κB/MAPK和PERK/Nrf2信号通路介导的氧化应激和炎症,减轻酒精性肝损伤
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-04-28 DOI: 10.1016/j.tice.2025.102913
Fang-fang Da , Yao-ting Meng , Yu-feng Chen , Zi-wan Yuan , Ying Liu , Zhong-hua Dai
{"title":"Tetracera asiatica flavonoids attenuate alcohol-induced liver injury by suppressing oxidative stress and inflammation mediated by the Keap-1/Nrf2/HO-1, NF-κB/MAPK and PERK/Nrf2 signaling pathways in alcoholic liver injury rats","authors":"Fang-fang Da ,&nbsp;Yao-ting Meng ,&nbsp;Yu-feng Chen ,&nbsp;Zi-wan Yuan ,&nbsp;Ying Liu ,&nbsp;Zhong-hua Dai","doi":"10.1016/j.tice.2025.102913","DOIUrl":"10.1016/j.tice.2025.102913","url":null,"abstract":"<div><div>Alcoholic liver disease is regarded as a leading reason for liver cirrhosis. This study aimed to investigate the protective effect of <em>tetracera asiatica flavonoids</em> (TAF) on alcoholic liver injury (ALI) and explore the associated mechanisms. An ALI rat model was established and then divided into four groups, including ALI group, low-dose TAF (l-TAF) group, medium-dose TAF (m-TAF) group, and high-dose TAF (h-TAF) group. Levels of ALT, AST, ALB, SOD, MDA, NO, CAT, TG, TNF-α, IL-1β, Nrf2, Keap1, HO-1, NQO-1, and GSH-Px were measured in ALI rats in different groups. Pathological changes and inflammatory infiltration were examined using HE staining. Western blot was used to detect expressions of Nrf2, MAPK p38, PERK, NF-κB, ERK1/2 and anti-JNK1/2/3. The results showed that TAF protected against alcoholic liver injury in ALI rats by decreasing ALT and AST levels and inhibiting inflammatory response. TAF significantly reversed alcohol-induced increase in NO (P &lt; 0.05), and remarkably decreased levels of TNF-α (<em>P</em> &lt; 0.001) and IL-1β (<em>P</em> &lt; 0.01), compared with the ALI group. TAF significantly increased the transcription of Nrf2, Keap1, HO-1, NQO-1 and GSH-Px gene (all P &lt; 0.05) and inhibited the alcohol-induced upregulation of MAPK p38 expression (<em>P</em> &lt; 0.001), p-NF-κB/NF-κB ratio (<em>P</em> &lt; 0.001), p-ERK/1/2/ERK1/2 ratio (<em>P</em> &lt; 0.05), and p-JNK1/2/3/JNK1/2/2 ratio (<em>P</em> &lt; 0.05), compared with the ALI group (all <em>P</em> &lt; 0.001). TAF obviously reversed effects of ALI modeling, and remarkably downregulated the expression of PERK and upregulated Nrf2 (all <em>P</em> &lt; 0.001) compared with the ALI rats. In conclusion, TAF attenuates alcohol-induced livery injury through suppressing Keap-1/Nrf2/HO-1, NF-κB/MAPK and PERK/Nrf2 signaling pathways mediated oxidative stress and inflammation in ALI rats.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102913"},"PeriodicalIF":2.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mechanism of EZH2-mediated histone methylation promoting bFGF-induced angiogenesis of human umbilical vein endothelial cells ezh2介导的组蛋白甲基化促进bfgf诱导的人脐静脉内皮细胞血管生成的机制
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-04-28 DOI: 10.1016/j.tice.2025.102945
Min Gao , Chen Xing
{"title":"Mechanism of EZH2-mediated histone methylation promoting bFGF-induced angiogenesis of human umbilical vein endothelial cells","authors":"Min Gao ,&nbsp;Chen Xing","doi":"10.1016/j.tice.2025.102945","DOIUrl":"10.1016/j.tice.2025.102945","url":null,"abstract":"<div><div>This study aims to explore the role of enhancer of zeste homolog 2 (EZH2)-mediated histone methylation in basic fibroblast growth factor (bFGF)-induced angiogenesis of human umbilical vein endothelial cells (HUVECs). EZH2, vascular endothelial growth factor A (VEGFA), miR-340–5p, and nuclear factor-erythroid 2-related factor 2 (NRF2) expressions in bFGF-induced HUVECs were detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. After transfection of EZH2 siRNA, NRF2 siRNA, or miR-340–5p inhibitor, cell migration and angiopoiesis were assessed by Transwell and tube formation assays. Chromatin immunoprecipitation (ChIP) was performed to analyze the enrichment of EZH2 or trimethylated H3 lysine 27 (H3K27me3) on NRF2 promoter. The binding between NRF2 and miR-340–5p was verified by ChIP and dual-luciferase assay. EZH2 was highly expressed while miR-340–5p and NRF2 were poorly expressed in bFGF-induced HUVECs. Silence of EZH2 restrained HUVEC migration, and reduced the number of branches and tube length. Mechanically, EZH2 enhances the enrichment of H3K27me3 on the NRF2 promoter, thereby repressing NRF2 expression and further leading to transcriptional repression of miR-340–5p. In conclusion, EZH2 inhibits the NRF2/miR-340–5p axis and promotes bFGF-induced angiogenesis of HUVECs by increasing the H3K27me3 modification on the NRF2 promoter.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102945"},"PeriodicalIF":2.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pro-apoptotic and mitochondria-disrupting effects of 4-methylthiazole in K562 leukemia cells: A mechanistic investigation 4-甲基噻唑在K562白血病细胞中的促凋亡和线粒体破坏作用:机制研究
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-04-27 DOI: 10.1016/j.tice.2025.102937
Neslihan Meriç , Ezgi Kar , Fatih Kar
{"title":"Pro-apoptotic and mitochondria-disrupting effects of 4-methylthiazole in K562 leukemia cells: A mechanistic investigation","authors":"Neslihan Meriç ,&nbsp;Ezgi Kar ,&nbsp;Fatih Kar","doi":"10.1016/j.tice.2025.102937","DOIUrl":"10.1016/j.tice.2025.102937","url":null,"abstract":"<div><div>Thiazole derivatives have garnered attention for their anticancer potential. This study investigates the antileukemic effects of 4-methylthiazole on K562 chronic myeloid leukemia (CML) cells, focusing on apoptosis induction and mitochondrial dysfunction. Cell viability was assessed using MTS assays; apoptosis and necrosis were analyzed via Annexin V/PI staining and flow cytometry; mitochondrial membrane potential changes were evaluated with JC-1 dye; gene expression levels were measured by qRT-PCR; and levels of apoptosis- and cytokine-related proteins were quantified using ELISA. Treatment with 4-methylthiazole led to selective cytotoxicity in K562 cells while sparing healthy peripheral blood mononuclear cells (PBMNCs). Apoptotic induction was evidenced by Caspase-3 (CASP-3) activation, Cytochrome-C (CYT-C), release, and mitochondrial depolarization. Gene expression analysis showed upregulation of pro-apoptotic markers such as <em>TP53</em> (tumor suppressor protein 53), <em>BAX</em> and <em>BAK</em> (pro-apoptotic Bcl-2 family proteins), while upregulation of <em>CASP3</em> (caspase-3) expression was not statistically significant. Conversely, levels of <em>GPX4</em> (glutathione peroxidase 4, involved in oxidative stress protection) remained unchanged, indicating an apoptosis mechanism independent of oxidative stress. Additionally, <em>SEMA3A</em> (Semaphorin 3 A, involved in tumor progression and cell signaling) was significantly downregulated. Cytokine profiling revealed a dose-dependent modulation of IL-6, while TNF-α and IL-10 levels remained unaffected. These findings suggest that 4-methylthiazole induces apoptosis through mitochondrial pathways, affects cytokine signaling, and selectively targets leukemia cells, supporting its potential as a therapeutic candidate for CML treatment.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102937"},"PeriodicalIF":2.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Resveratrol protects against letrozole-induced renal damage in a rat model of polycystic ovary syndrome: A biochemical, histological, and immunohistochemical study 白藜芦醇对多囊卵巢综合征大鼠模型来曲唑诱导的肾脏损伤有保护作用:生化、组织学和免疫组织化学研究
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-04-26 DOI: 10.1016/j.tice.2025.102934
Einas M. Yousef , Samar M. Abd El-moneam , Shimaa Mohammad Yousof , Safaa Abdallah Mohammed , Basma Osman Sultan , Basma S.A. Mansour
{"title":"Resveratrol protects against letrozole-induced renal damage in a rat model of polycystic ovary syndrome: A biochemical, histological, and immunohistochemical study","authors":"Einas M. Yousef ,&nbsp;Samar M. Abd El-moneam ,&nbsp;Shimaa Mohammad Yousof ,&nbsp;Safaa Abdallah Mohammed ,&nbsp;Basma Osman Sultan ,&nbsp;Basma S.A. Mansour","doi":"10.1016/j.tice.2025.102934","DOIUrl":"10.1016/j.tice.2025.102934","url":null,"abstract":"<div><h3>Background</h3><div>Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects 9–18 % of women, often associated with metabolic and renal complications. This study aimed to investigate the renoprotective effects of resveratrol (RSV) in a letrozole-induced rat model of PCOS, focusing on biochemical, histological, ultrastructural, and immunohistochemical alterations.</div></div><div><h3>Methods</h3><div>Thirty female rats were randomly divided into five groups: negative control, only RSV-treated, PCOS-induced (sham), metformin-treated, and RSV-treated. Serum testosterone, urea, and creatinine levels were assessed. Renal tissues underwent histological, immunohistochemical, ultrastructural, and morphometric analyses. Additionally, TGF-β1 mRNA expression was evaluated using qRT-PCR.</div></div><div><h3>Results</h3><div>Letrozole administration significantly elevated serum testosterone, urea, and creatinine levels, indicating PCOS-associated renal dysfunction. Histological and ultrastructural analysis revealed severe glomerular and tubular alterations in the sham group. The administration of RSV significantly restored renal architecture and function more effectively than metformin. Immunohistochemistry analysis showed that RSV reduced Proliferating Cell Nuclear Antigen (PCNA) overexpression and restored B-cell Lymphoma 2 (BCL-2) expression, suggesting a protective effect against cellular stress and apoptosis. Moreover, RSV significantly downregulated TGF-β1 expression, indicating its anti-fibrotic and anti-inflammatory role in PCOS-related renal damage.</div></div><div><h3>Conclusion</h3><div>In a PCOS rat model, RSV protects effectively against letrozole-induced structural and functional renal damage. It demonstrates superior efficacy over metformin in restoring renal function, reducing apoptosis, and mitigating fibrosis. These findings suggest that RSV may serve as a potential adjunct therapy for preventing PCOS-associated renal complications, emphasizing the need for further investigations.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102934"},"PeriodicalIF":2.7,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modulation of TLR4 mediated HMGB1/RAGE/NF-κB axis through linarin against fenvalerate provoked cardiotoxicity 通过linarin调节TLR4介导的HMGB1/RAGE/NF-κB轴抗氰戊酸引起的心脏毒性
IF 2.7 4区 生物学
Tissue & cell Pub Date : 2025-04-26 DOI: 10.1016/j.tice.2025.102931
Fuad M. Alzahrani , Arifa Mehreen , Qurat Ul Ain , Adnan Ali , Khalid J. Alzahrani , Khalaf F. Alsharif
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