Tissue & cell最新文献

{"title":"Cirsiliol confers cardio-protection against sunitinib induced cardiotoxicity via synergistic modulation of SIRT1/FOXO3a and endothelin axis: A biochemical, histopathological, and computational experimentation","authors":"Mahmoud El Safadi ,&nbsp;Abdulaziz Saleh Alkhoshaiban ,&nbsp;Hafsa Ashfaq ,&nbsp;Giorgio Antoniolli ,&nbsp;Ahmed Al-Emam ,&nbsp;Hesham M. Hassan","doi":"10.1016/j.tice.2025.103129","DOIUrl":"10.1016/j.tice.2025.103129","url":null,"abstract":"<div><div>Cardiotoxicity remains a major clinical challenge associated with various environmental and chemotherapeutic toxicants. Sunitinib (SNB) is a potent targeted cancer drug that is reported to induce severe organ damage including renal failure. Cirsiliol (CSL) is a natural flavone that exhibits marvelous pharmacological properties. In this investigation, we explored the potential cardioprotective nature of CSL to counter SNB induced cardiac impairments. Sprague Dawley rats (n = 36) were categorized into control, SNB (25 mgkg⁻¹), SNB (25 mgkg⁻¹) + CSL (10 mgkg⁻¹), and CSL (10 mgkg⁻¹) alone experimented group. SNB exposure led to a notable reduction in the expression of Endothelin Receptor Type B (EDNRB), Forkhead box O3a (FOXO3a), and sirtuin 1 (SIRT1) while exacerbating the expression of P21, P53, Endothelin-1 (EDN-1), Endothelin Receptor Type A (EDNRA). The levels of reactive oxygen species (ROS) and malondialdehyde (MDA) were promoted while the enzymatic activities of hemeoxygenase-1 (HO-1), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GSR), catalase (CAT), and glutathione (GSH) contents were reduced following the SNB exposure. Moreover, SNB intoxication led to a marked elevation in the concentrations of C-reactive protein, creatine kinase-myocardial band (CK-MB), Pro-B-Type natriuretic peptide (ProBNP), troponin-T, Lactate dehydrogenase (LDH), Creatine phosphokinase (CPK), troponin-I and Brain natriuretic peptide (BNP). Cardiac tissues showed sever immune-inflammatory responses after SNB intoxication as confirmed by augmented levels and expressions of cyclooxygenase-2 (COX-2), tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and total fraction of nuclear factor-kappa B (NF-κB). Furthermore, the SNB administration upregulated the concentrations of cysteine-aspartic proteases-9 (Caspase-9), cysteine-aspartic proteases-3 (Capase-3), and Bcl-2–associated X protein (Bax) while declining the concentration of B-cell lymphoma 2 (Bcl-2). SNB intoxication caused histological disarrays including myofibrillar degeneration, capillary dilation, wavy fibers, necrosis of focal regions, hypertrophy of cardiomyocytes, inflammation and interstitial edema. Nonetheless, CSL therapy notably reversed these pathological changes via upregulating SIRT1/FOXO3a and endothelin pathways while reducing cardiac inflammation, apoptosis and cardiac function markers. Our results are validated through in-silico which showed that CSL showed high binding affinity with key regulatory pathways.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103129"},"PeriodicalIF":2.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scanning electron microscopy and morphometric analysis of the tongue in four carnivorous fish species—Dusky Grouper (Epinephelus marginatus), John Dory (Zeus faber), Squirrelfish (Sargocentron spinosissimum), and Red Lionfish (Pterois miles)","authors":"Samir A.A. El-Gendy ,&nbsp;Mohamed A.M. Alsafy ,&nbsp;Neveen E.R. El-Bakary ,&nbsp;Ahmed A. El-Mansi ,&nbsp;Mamdouh B. Eldesoqui ,&nbsp;Amal F. Dawood ,&nbsp;Soha Soliman ,&nbsp;Basma M. Kamal ,&nbsp;Ahmed M. Rashwan","doi":"10.1016/j.tice.2025.103120","DOIUrl":"10.1016/j.tice.2025.103120","url":null,"abstract":"<div><div>The Mediterranean Sea is home to a wide variety of fish species that exhibit carnivorous behavior, particularly during the juvenile to pre-adult stages. This study aimed to compare the tongue morphology of four Mediterranean carnivorous fish species: the dusky grouper fish (<em>Epinephelus marginatus</em>), John Dory fish (<em>Zeus faber</em>), squirrelfish (<em>Holocentrus</em> spp.), and red lionfish (<em>Pterois volitans</em>). To our knowledge, no previous studies have conducted a comparative analysis of tongue morphology among these species, an important area of investigation for understanding species-specific adaptations related to feeding behavior, prey capture mechanisms, and ecological niches. We examined morphometric parameters, gross anatomical features, and scanning electron microscopic (SEM) characteristics of the dorsal tongue surface. Among the species studied, the John Dory fish had the highest body depth-to-length ratio (38.8 %), while the squirrelfish showed the highest eye diameter-to-head length ratio (12.2 %) and the red lionfish the lowest (3.04 %). The dusky grouper fish exhibited the highest tongue-to-body length ratio. In contrast, the red lionfish had a shorter tongue with a broad root nearly equal in size to the tongue itself. All species had triangular tongues, but only the red lionfish showed a constriction before the apex. The tongue apex differed among species: it was rounded in the dusky grouper, pointed in both the squirrelfish and red lionfish, and featured a distinct structure in the John Dory fish. The dorsal tongue surface exhibited species-specific features, including elevations, ridges, grooves, and unique apical structures. SEM analysis revealed microridges, folds, multiple papilla types, and taste buds. The dusky grouper displayed volcano-shaped taste buds and circumvallate papillae; the John Dory fish showed rosette-shaped protrusions and circumvallate-like papillae; the squirrelfish had scale-like and dome-shaped papillae; and the red lionfish revealed elliptical and dome-shaped structures with microgrooves and mucus secretion. In conclusion, variations in tongue morphology and its macro- and microstructures likely reflect differences in prey type, feeding behavior, and ecological adaptation, and this study lays the groundwork for future comparative research on Mediterranean fish.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103120"},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective role of pumpkin seed oil in colchicine-treated rats through redox balance, apoptosis modulation, and anti-fibrotic effects","authors":"Yasmina M. Abd-Elhakim ,&nbsp;Ezzat F.F. Soliman ,&nbsp;Manar A. AbdelMageed ,&nbsp;Azza M.A. Abo-Elmaaty ,&nbsp;Taghred M. Saber ,&nbsp;Nabela I. El-Sharkawy ,&nbsp;Gihan G. Moustafa ,&nbsp;Fathy Elsayed Abdelgawad ,&nbsp;Engy Mohamed Mohamed Yassin","doi":"10.1016/j.tice.2025.103128","DOIUrl":"10.1016/j.tice.2025.103128","url":null,"abstract":"<div><div>Colchicine (CNE) is used to treat various inflammatory conditions, but its use is limited by its hepatotoxic potential. This study assessed the protective effect of pumpkin seed oil (PPN) against CNE-induced liver injury in rats. Forty male Sprague-Dawley rats were allocated into four groups: control, PPN (4 mL/kg b.wt), CNE (0.6 mg/kg b.wt), and CNE+PPN, and received oral treatments for 56 days. CNE significantly elevated liver enzymes (ALP, GGT, AST, and ALT) and bilirubin levels, and induced dyslipidemia, while PPN co-treatment restored these parameters toward normal. Antioxidant defenses, including SOD, CAT, and GSH were significantly suppressed by CNE, accompanied by increased MDA, protein carbonyls, 8-OHdG, and caspase-3 activity. PPN treatment improved redox status and reduced apoptotic markers. Histologically, CNE caused ductular reactions, bile duct hyperplasia, and collagen fiber accumulation around the portal triads. Masson's trichrome staining confirmed significant fibrosis, which was significantly reduced with PPN co-administration. Immunohistochemistry revealed a significant reduction in PCNA in CNE-treated livers, partially restored by PPN. BAX expression was significantly increased by CNE and notably reduced in the CNE+PPN group. These findings highlight the hepatoprotective potential of PPN through antioxidant, anti-apoptotic, anti-fibrotic, and lipid-regulating mechanisms, supporting its use as a natural beneficial agent against CNE-induced liver injury.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103128"},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ex vivo mechanical and microstructural evaluation of a changing hepatic microstructure","authors":"Alexander Chan Kai Foong ,&nbsp;Claire Dempsey ,&nbsp;Brooke Tornifoglio","doi":"10.1016/j.tice.2025.103121","DOIUrl":"10.1016/j.tice.2025.103121","url":null,"abstract":"<div><h3>Background/objectives</h3><div>Recent advancements in non-invasive imaging of the liver hold significant potential for disease diagnosis and monitoring. However, the influence of different microstructural features on clinically derived mechanical biomarkers in the liver are not fully elucidated. The aim of this study was to investigate the influence of microstructural changes within liver tissue on mechanics.</div></div><div><h3>Methods</h3><div>The impact of storage and preservation, as well as bulk microstructural changes via enzymatic treatments were evaluated by mechanical testing and histological processing on porcine liver.</div></div><div><h3>Results</h3><div>We found that the preservation method chosen for <em>ex vivo</em> liver tissue significantly influences the compressive material properties of the tissue, while not impacting those in tension. Additionally, we found that enzymatic treatments via collagenase alter the microstructure and mechanics, again more significantly in compression, of liver tissue.</div></div><div><h3>Conclusion</h3><div>This work lays foundational insights for future studies which aim to develop <em>ex vivo</em> liver models to better understand the changing microstructure in the liver and its influence on mechanics, ultimately improving our understanding of clinically derived biomarkers.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103121"},"PeriodicalIF":2.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological-based characterization of ovarian developmental stages in blue crab (Callinectes sapidus) within the spawning season in the central western Adriatic Sea","authors":"Giulia Chemello ,&nbsp;Elena Giannico ,&nbsp;Matteo Zarantoniello ,&nbsp;Erica Trotta ,&nbsp;Laura Ciaralli ,&nbsp;Marco Matiddi ,&nbsp;Cecilia Silvestri ,&nbsp;Ludovica Di Renzo ,&nbsp;Loredana Manfra ,&nbsp;Giovanni Libralato ,&nbsp;Ike Olivotto ,&nbsp;Giorgia Gioacchini","doi":"10.1016/j.tice.2025.103118","DOIUrl":"10.1016/j.tice.2025.103118","url":null,"abstract":"<div><div>The Atlantic blue crab (<em>Callinectes sapidus</em> Rathbun, 1896) is a euryhaline and eurythermal species native to the Atlantic coasts of the Americas. Although its widespread distribution across the Mediterranean basin is well documented, information on its reproductive patterns remains limited. This study focused on the combination of both macroscopic and histologic characterization of ovarian developmental stages in female blue crabs along the north-central Italian Adriatic coast, within the spawning period. Samples were collected off the coast of Ancona, in collaboration with local fishermen, between September and November 2023. For each specimen, sex and biometric parameters were recorded to determine the sex ratio, while size distribution and gonadosomatic index (GSI) were calculated for female specimens. Ovary samples and eggs sponge were collected to determine the ovarian developmental stage and fecundity respectively. Results showed females as the dominant sex throughout the sampling period, with males being extremely rare. The collapse of female catches in early November, alongside the consistent presence of ovigerous females and the strong female-biased sex ratio, identified this area as a spawning ground. These findings align with reproductive patterns observed in the species’ native range and other Mediterranean areas. Moreover, GSI values and fecundity indexes associated with macroscopic and histological examination of ovarian stages suggested that the end of the reproductive season corresponded to mid-November.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103118"},"PeriodicalIF":2.5,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eggshell membrane supplementation stimulates angiogenesis and promotes bone healing in a rat drill-hole injury model","authors":"Nao Yashima ,&nbsp;Wataru Minamizono ,&nbsp;Hirai Suito ,&nbsp;Takumi Okunuki ,&nbsp;Kaoru Fujikawa ,&nbsp;Shingo Nakai ,&nbsp;Masafumi Ohsako","doi":"10.1016/j.tice.2025.103117","DOIUrl":"10.1016/j.tice.2025.103117","url":null,"abstract":"<div><div>The development of new adjunct therapies to support bone healing remains an important clinical challenge. Eggshell membrane (ESM), a natural biomaterial derived from chicken eggs, has recently attracted attention for its safety, biocompatibility, and cost-effectiveness. We aimed to evaluate the effects of ESM supplementation on bone healing in a rat tibial drill-hole injury model. Seven-week-old male Wistar rats, acclimated for one week prior to the experiment, were divided into three groups: control, bone injury only, and bone injury with ESM supplementation (EM). Micro-computed tomography and histological analyses revealed a significant increase in new bone volume in the EM group at days 14 and 21 post-drill hole injury. On day 21, reduced toluidine blue staining, a lower osteocyte count, and the maturation of collagen fibers. Moreover, <em>Sp7</em> mRNA expression and the number of osteocalcin-positive cells were increased. The expression of <em>Nfatc1</em> and the number of tartrate-resistant acid phosphatase-positive osteoclasts were also significantly elevated. In addition, the vascular area and <em>Vegfa</em> expression were markedly increased in the EM group throughout the observation period. These findings suggest that ESM supplementation promotes bone healing by stimulating angiogenesis and enhancing osteoblast and osteoclast activity, offering a promising nutritional adjunct to support and accelerate fracture healing.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103117"},"PeriodicalIF":2.5,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioengineered chitosan/silk scaffold encapsulated with quercetin nanoparticles accelerates wound healing in a diabetic rat skin defect model","authors":"Suad Hamdan Almasoudi","doi":"10.1016/j.tice.2025.103119","DOIUrl":"10.1016/j.tice.2025.103119","url":null,"abstract":"<div><div>Chronic wounds, particularly in diabetic patients, are characterized by prolonged inflammation, impaired angiogenesis, and delayed tissue regeneration. To address these challenges, the author developed a bioactive scaffold by incorporating quercetin nanoparticles (Qn) into a chitosan/silk fibroin (ChS) matrix, aiming to accelerate and enhance the wound healing process. Quercetin nanoparticles were synthesized via a solvent displacement method and incorporated into a ChS scaffold using a blending and freeze-drying technique. The physicochemical properties of the scaffold, including porosity, swelling ratio, degradation profile, biocompatibility, mechanical properties, and drug release kinetics, were characterized. Full-thickness excisional wounds (20 mm) were created on the dorsum of streptozotocin-induced diabetic Wistar rats, which were divided into three groups (n = 5): untreated control, ChS, and Qn-loaded ChS (QnChS). Wound closure was monitored on days 7 and 14. Histological evaluations (H&amp;E and Masson's Trichrome) assessed tissue regeneration, vascularization, and collagen deposition. Cytokine levels (bFGF, VEGF, TNF-α, IL-1β) were quantified using ELISA. The QnChS scaffolds displayed a porous structure with sustained drug release over 14 days and enhanced biodegradability. In vivo, the QnChS group showed significantly accelerated wound contraction, increased dermal thickness and epidermal length, and higher collagen deposition compared to the other groups (p &lt; 0.05). Angiogenesis was notably improved, evidenced by a higher number of blood vessels and elevated VEGF levels. Additionally, QnChS scaffolds markedly reduced TNF-α and IL-1β while increasing bFGF expression, indicating enhanced resolution of inflammation and promotion of tissue regeneration. The Qn-loaded ChS scaffold effectively modulates the inflammatory response, promotes angiogenesis, and enhances dermal regeneration in diabetic wounds. These findings suggest its potential as a multifunctional wound dressing for managing chronic wounds in diabetic patients.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103119"},"PeriodicalIF":2.5,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ark shell-derived peptides AWLNH (P3) and PHDL (P4) as novel inhibitors of endothelial dysfunction in Cardiovascular disease models","authors":"Chathuri Kaushalya Marasinghe ,&nbsp;Lakshi Ayoda Dayarathne ,&nbsp;Jae-Young Je","doi":"10.1016/j.tice.2025.103116","DOIUrl":"10.1016/j.tice.2025.103116","url":null,"abstract":"<div><div>Endothelial dysfunction is a main early event in the onset of atherosclerosis and cardiovascular diseases. This study explores the ameliorating effects of ark shell-derived multifunctional peptides, AWLNH (P3) and PHDL (P4), against oxLDL-driven endothelial dysfunction in human umbilical vein endothelial cells (HUVECs). P3 and P4 significantly improved cell viability, enhanced nitric oxide levels, and upregulated endothelial nitric oxide synthase expression while suppressing oxLDL-stimulated lectin-like oxidized low-density lipoprotein receptor-1 expression. Additionally, P3 and P4 exhibited potent antioxidant activity by lowering intracellular ROS levels and lipid peroxidation, and increasing antioxidant enzyme activity. Furthermore, P3 and P4 inhibited oxLDL-induced endothelial cell apoptosis by modulating Bax/Bcl-2 ratio and preserving mitochondrial membrane potential. Moreover, P3 and P4 mitigated vascular inflammation through downregulating intercellular adhesion molecule-1 and vascular cell adhesion molecule-1expression. Our findings showed that P3 and P4 ameliorate oxLDL-induced endothelial dysfunction, providing insights into bioactive peptide-based therapeutics in vascular therapy.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103116"},"PeriodicalIF":2.5,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STAT4 recruitment following DNA demethylation in MMP-3 promoter leads to MMP-3 expression activation in articular cartilage of osteoarthritis mice","authors":"Dechao Kong,&nbsp;Lei Cao,&nbsp;Huichao Fu,&nbsp;Xiaofeng Wu","doi":"10.1016/j.tice.2025.103115","DOIUrl":"10.1016/j.tice.2025.103115","url":null,"abstract":"<div><div>Osteoarthritis (OA) is a multifactorial joint disease that encompasses not just cartilage deterioration but also meniscal degeneration, subchondral bone remodeling, and fibrosis of the infrapatellar fat pad and synovial membrane. OA is characterized by a progressive and irreversible loss of the articular cartilage, due in main part to the cleavage of type II collagen within the matrix by the enzyme matrix metalloproteinases (MMPs). As an important member of MMP family, MMP-3 plays a prominent role in the pathologenesis of OA. The pathological factors and signaling pathways mediating <em>Mmp-3</em> expression activation during OA development are not well defined and have been a focus of intense research. <em>Mmp-3</em> promoter is featured by CpG sites. So far there have been no reports concerning the functional role of DNA methylation in <em>Mmp-3</em> promoter during OA development. In this study, we sought to investigate DNA methylation status in <em>Mmp-3</em> promoter, the role of DNA methylation in <em>Mmp-3</em> activation in OA, and the underlying mechanisms. Our results showed that <em>Mmp-3</em> promoter demethylation played an important role in <em>Mmp-3</em> expression activation in OA, which facilitated signal transducer and activator of transcription 4 (STAT4) binding on <em>Mmp-3</em> promoter to activate <em>Mmp-3</em> expression. More importantly, OA pathological phenotype of mice was alleviated in response to siRNA-mediated STAT4 knockdown. Our study illustrates how subtle changes in DNA methylation/demethylation status can directly influence gene expression and contribute to pathology and takes us one step further in the understanding of the pathogenesis of OA.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103115"},"PeriodicalIF":2.5,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanosphere loaded with curcumin attenuates adipogenesis and lipid metabolism by modulating AKT/mTORC1/PPARγ phosphorylation","authors":"Ji-Yeon Park ,&nbsp;Ri-Won Kim ,&nbsp;Jin Lee ,&nbsp;Won-Kyo Jung ,&nbsp;Jae-Young Je ,&nbsp;Sei-Jung Lee","doi":"10.1016/j.tice.2025.103112","DOIUrl":"10.1016/j.tice.2025.103112","url":null,"abstract":"<div><div>Curcumin, a hydrophobic polyphenol derived from turmeric, exhibits diverse biological activities as a natural supplement. However, its low gastrointestinal absorption significantly limits its oral bioavailability. In this study, we investigated the effects of curcumin-loaded nanospheres (CN) on adipocyte differentiation in 3T3-L1 cells and obesity development in high-fat diet-induced obese mice. CN markedly suppressed adipogenesis in 3T3-L1 preadipocytes, demonstrating approximately 100-fold greater anti-adipogenic activity compared to native curcumin. Mechanistically, CN inhibited mTOR phosphorylation by modulating intracellular reactive oxygen species during adipocyte differentiation. Notably, CN restored fatty acid synthase expression through regulation of PPARγ phosphorylation. In high-fat diet-fed mice, oral administration of CN significantly reduced body weight without altering food intake. Furthermore, CN alleviated the accumulation of white adipose tissue and liver mass, along with a reduction in plasma total cholesterol levels. Collectively, these findings suggest that CN enhances the bioavailability of curcumin and exerts potent anti-obesity effects by targeting adipogenesis and lipid metabolism.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"97 ","pages":"Article 103112"},"PeriodicalIF":2.5,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}