Tissue & cell最新文献

{"title":"Differential expression of GABARAPs in glioblastoma renders temozolomide sensitivity in a p53-dependent manner","authors":"Megha Chaudhary ,&nbsp;Naveen Soni ,&nbsp;Nargis Malik ,&nbsp;Kunzang Chosdol ,&nbsp;Bhawana Bissa","doi":"10.1016/j.tice.2025.102991","DOIUrl":"10.1016/j.tice.2025.102991","url":null,"abstract":"<div><div>Glioblastoma is one of the most debilitating and extremely aggressive tumors with a median survival of less than a year. Glioblastoma have high metastatic potential and frequently acquire chemoresistance. The current multimodal treatment approaches for glioblastoma include surgical tumor resurrection, radiotherapy, and chemotherapy but these approaches leave the patient with long-term disabilities such as depletion of cognitive abilities, leukoencephalopathy, and recurrence in 6–8 months. Glioma cells are highly dependent on autophagy to survive and proliferate. Autophagy inhibition is proved to be a beneficial strategy for restricting glioma growth. However, due to the lack of specific autophagy inhibitors, the autophagy pathway cannot be efficiently targeted. Understanding the vulnerabilities in autophagy gene expression can help to design better autophagy inhibitors. This study demonstrates the differential expression of GABARAP family members in glioblastoma. Our study highlights the differential expression of GABARAP family members in response to autophagy inhibition and induction. Moreover, the knockdown of specific GABARAP family members enhanced proliferation and reduced temozolomide (TMZ) sensitivity of glial cells by decreasing the p53 expression. The selective expression pattern of GABARAP genes in glioblastoma can be utilized to screen for patients who might respond better to temozolomide treatment. The differential expression of GABARAP family members highlights the subtle regulation of the autophagy pathway in response to environmental cues.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102991"},"PeriodicalIF":2.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anterior cruciate ligament reconstruction affects the development of muscle atrophy depending on its timing in rats","authors":"Akinori Kaneguchi ,&nbsp;Marina Kanehara ,&nbsp;Norikazu Nishida ,&nbsp;Kaoru Yamaoka ,&nbsp;Junya Ozawa","doi":"10.1016/j.tice.2025.102992","DOIUrl":"10.1016/j.tice.2025.102992","url":null,"abstract":"<div><div>After anterior cruciate ligament (ACL) reconstruction, muscle atrophy is frequently observed. No conclusions have been reached on the effects of timing of surgery on muscle atrophy. We aimed to determine the best timing of ACL reconstruction surgery to minimize muscle atrophy. Eight-week-old male Wistar rats were used. After 1, 14, or 28 days of ACL transection, rats underwent ligament reconstruction. Some ACL-transected rats were reared without ligament reconstruction. Untreated rats served as controls. At 56 days after ACL transection, muscle atrophy in the rectus femoris and gastrocnemius were assessed. There were no significant differences in any of the parameters relevant to muscle atrophy between the control and ACL-transected rats. ACL reconstruction did not significantly change the parameters relevant to muscle atrophy in the rectus femoris, regardless of timing. In the gastrocnemius, ACL reconstruction resulted in a reduction in slow fiber cross-sectional area (CSA) and an increase in the proportion of fast fiber in the deep region, but not in the superficial region, regardless of timing. When reconstruction was performed at 28 days, a reduction in fast fiber CSA in the deep region was also detected. In conclusion, ACL reconstruction did not alter atrophic parameters in the rectus femoris, while it caused slow fiber atrophy and an increase in the proportion of fast fiber in the deep region of the gastrocnemius, regardless of timing. However, delayed reconstruction surgery developed fast fiber atrophy as well in the deep region of the gastrocnemius. Thus, reconstruction surgery should be performed as soon as possible.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102992"},"PeriodicalIF":2.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondria transfer from mesenchymal stem cells into osteoarthritic chondrocytes ameliorate cellular functions and alleviate both inflammation and oxidative stress","authors":"Candan Altuntaş ,&nbsp;Gökhan Duruksu ,&nbsp;Fatih Hunç ,&nbsp;Yusufhan Yazir","doi":"10.1016/j.tice.2025.102990","DOIUrl":"10.1016/j.tice.2025.102990","url":null,"abstract":"<div><h3>Objective</h3><div>Osteoarthritis, a common age-related joint disease, causes cartilage degeneration, leading to pain and disability. While pain management exists, cartilage regeneration options are limited. Exogenous mitochondria transfer is a novel regenerative approach. This study aimed to investigate the effects of exogenous mitochondrial transfer on cellular function, oxidative stress, inflammation, and apoptosis in osteoarthritic chondrocytes.</div></div><div><h3>Methods</h3><div>Two inflammatory models using M1-macrophage conditioned medium or co-culture with synovial fluid mesenchymal stem cells (MSCs) were established. The study compared mitochondria from Wharton's jelly (WJ-) and bone marrow (BM-) MSCs by analyzing their transfer to these models. Transfer effects were evaluated by mitochondrial membrane potential, cell viability, apoptosis, gene expression, and oxidative state.</div></div><div><h3>Results</h3><div>Mitochondria tracking showed high transfer efficiencies (99.62 % for WJ-MSCs, 91.34 % for BM-MSCs). Late apoptosis was significantly reduced after transfer of WJ-MSCs mitochondria from 5.58 % to 2.93 % in the model with M1-macrophage conditioned medium. Expression of TNF-α and IL-1β was reduced after mitochondrial delivery. The expression of Ki67 was induced in parallel with increased ATP production and reduced HMOX-1 expression levels after the transfer. A decrease of 2.5- and 5-fold in ATP levels in cells after the inflammatory models were recovered after WJ-MSCs mitochondria transfer by 3.1- and 100-fold depending on the inflammatory model used. Although ROS levels remained unchanged, MDA levels decreased, and collagen type-2 expression increased.</div></div><div><h3>Conclusion</h3><div>Mitochondria transfer improved key aspects of chondrocyte dysfunction in inflammatory osteoarthritis models. These findings support its therapeutic potential for treating or slowing osteoarthritis by directly improving damaged chondrocyte health and function.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102990"},"PeriodicalIF":2.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Omega-3's impact on postnatal cerebral cortex development: A histological, immuno-histochemical, and biochemical study in a valproic acid rat model","authors":"Mohammed Ahmed Shehata ,&nbsp;Alaa Fawzy Abdelaal ,&nbsp;Noha Emad Abd El Fattah Eladawy ,&nbsp;Nancy Husseiny Hassan","doi":"10.1016/j.tice.2025.102989","DOIUrl":"10.1016/j.tice.2025.102989","url":null,"abstract":"<div><h3>Introduction</h3><div>The anti-epileptic medication Valproic acid (VPA) can damagedamage DNA and interfere with the mitochondria's capacity to metabolize energy. Consequently, VPA is a strong teratogen for the progeny of expectant mothers. OM3FA's capacity to preserve the cell membrane integrity of neural tissues improves cognitive function and Alzheimer's disease.</div></div><div><h3>Aim of work</h3><div>To determine postnatal neuroprotective impact of Omega-3 on cerebral cortex in Valproic acid-treated rat model.</div></div><div><h3>Material and methods</h3><div>Forty adult pregnant female albino rats were randomized into 4 groups equally: the control negative group (group I) which didn’t receive any medications, the Omega3 treated group (group II) which received Omega3 (144 mg/Kg/ day) orally once daily and the Valproic Acid treated group (group III) which received Valproic Acid oral solution (50 mg/Kg/day) once daily using gastric tubes and the Valproic Acid +Omega3 treated group received VPA(50 mg/Kg/day) and Omega3(144 mg/Kg/ day) once daily. Then the cerebral cortex of all offspring were processed at 7th and 21th day after birth to be evaluated biochemically, histologically, immunohistochemically, and morphometrically.</div></div><div><h3>Results</h3><div>Histologically and immunohistochemically, the group treated with Valproic Acid revealed remarkable degenerative and neuronal apoptotic changes of the cerebral cortex six layers. The group treated with Valproic Acid+Omega3 showed enhancement in the histological, and immunohistochemical alterations. As regards the biochemical (MDA &amp;TAC) and morphometric results Valproic Acid+Omega3 showed statistically significant improvement in comparison with the Valproic Acid.</div></div><div><h3>Conclusion</h3><div>Valproic acid consumption during pregnancy documented to have neurotoxic impact on cerebral cortex structure of the rat offspring. Omega3 has a protective effect against Valproic acid neurotoxicity.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102989"},"PeriodicalIF":2.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing thrombospondin function through repositioning: Implications for injury and diabetic foot ulcer management","authors":"Juan Valentin Trujillo-Paez ,&nbsp;Yolanda M. Jacobo-Delgado ,&nbsp;Camelia Felix-Arellano ,&nbsp;Adrian Rodriguez-Carlos ,&nbsp;Irma Gonzalez-Curiel ,&nbsp;Oscar Gonzalez-Muñiz ,&nbsp;Bruno Rivas-Santiago","doi":"10.1016/j.tice.2025.102987","DOIUrl":"10.1016/j.tice.2025.102987","url":null,"abstract":"<div><div>Diabetic foot ulcer (DFU) is a common and challenging complication of diabetes, characterized by impaired wound healing, chronic inflammation, poor tissue remodeling, and chronic inflammation. Thrombospondin 1 (TSP1) and thrombospondin 4 (TSP4) are key extracellular matrix proteins involved in wound healing. This study aimed to assess the levels and propose inducers of TSP1, TSP4, and SOD3 in diabetic foot ulcer. In this study, we first evaluated the expression of TSP1, TSP4, and superoxide dismutase 3 (SOD3) in DFU tissues using real-time PCR and immunohistochemistry. In DFU tissues using real-time PCR we found that TSP-4 expression was significantly reduced and confirmed by immunohistochemistry. TSP1 expression did not show similar alterations. Additionally, while SOD3 expression was decreased at the mRNA level in the diabetic population, no changes were observed in the protein levels by immunohistochemistry. To explore potential therapeutic approaches, we performed pharmacological repositioning and identified three drugs riboflavin, desloratadine, and chenodeoxycholic acid that selectively increased TSP-4 expression. These findings suggest that riboflavin, desloratadine, and chenodeoxycholic acid may promote wound healing in DFU by specifically upregulating TSP4, potentially enhancing tissue remodeling and angiogenesis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102987"},"PeriodicalIF":2.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preventing effects of anterior cruciate ligament reconstruction on the trabecular bone remodeling in the rat proximal tibial epiphysis","authors":"Akinori Kaneguchi ,&nbsp;Marina Kanehara ,&nbsp;Norikazu Nishida ,&nbsp;Kaoru Yamaoka ,&nbsp;Junya Ozawa","doi":"10.1016/j.tice.2025.102985","DOIUrl":"10.1016/j.tice.2025.102985","url":null,"abstract":"<div><div>A previous study reported that in patients with anterior cruciate ligament (ACL) injury, subchondral bone density in the proximal tibia was reduced in the antero-medial region, while it was increased in the postero-medial region. Anterior tibial instability due to ACL injury may be the cause of the remodeling in the proximal tibial epiphysis. The aim of this study was to determine whether re-stabilization of tibial instability by ACL reconstruction prevents remodeling in the rat proximal tibial epiphysis and if so, to find optimal timing of ACL reconstruction after ACL transection. ACL-transected rats were divided into immediate, early, and delayed ACL reconstruction (1, 14, and 28 days after ACL transection, respectively) groups. Some ACL-transected rats were reared without ligament reconstruction. Untreated rats were used as controls. At 56 days after ACL transection, trabecular bone mass in the proximal tibia was assessed histologically by measuring the trabecular bone area. ACL transection decreased the trabecular bone area in the antero-medial region of the proximal tibia, while it did not alter trabecular bone area in the postero-medial region. This trabecular bone loss in the antero-medial region was prevented when reconstruction was performed 1 day after ACL transection, but not prevented by ACL reconstruction performed in later period, 14 or 28 days after ACL transection. Our results suggest that ACL reconstruction surgery should be performed as early as possible to prevent trabecular bone loss in the antero-medial region of the proximal tibia.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102985"},"PeriodicalIF":2.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144107095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbutin improves post-myocardial infarction cardiac dysfunction by inhibiting cardiac fibroblast activation","authors":"Yun Liu ,&nbsp;Xuemei Liu ,&nbsp;Xuan Zhang ,&nbsp;Hongwei Yue ,&nbsp;Chang Pan","doi":"10.1016/j.tice.2025.102986","DOIUrl":"10.1016/j.tice.2025.102986","url":null,"abstract":"<div><h3>Background</h3><div>Arbutin exhibits multiple effects, including anti-inflammatory, antioxidant, and scavenging of free radicals, yet its research within the cardiovascular system remains limited.</div></div><div><h3>Purpose</h3><div>This study aims to investigate the effects of arbutin on myocardial infarction induced by left anterior descending (LAD) ligation in mice.</div></div><div><h3>Methods</h3><div>The possible mechanism of arbutin's effect on myocardial infarction was predicted through network pharmacology studies. To evaluate the impact of arbutin on cardiac function in myocardial infarction, TTC staining of the heart was performed, and echocardiography was conducted on mice at different time points. Furthermore, Western blot analysis was utilized not only to detect αSMA, Collagen I, and Collagen III, aiming to investigate the impact of arbutin on myocardial fibrosis but also to detect the influence of arbutin on the phosphorylation level of ERK1/2, thereby elucidating its potential mechanism of action.</div></div><div><h3>Results</h3><div>Network pharmacology data suggest that arbutin may exert a beneficial effect on myocardial infarction by modulating the ERK1/2 signaling pathway. In vivo experimental results indicate that after myocardial infarction, arbutin can alleviate myocardial fibrosis and cardiac hypertrophy, significantly improving cardiac function. Further in vitro experiments confirm that arbutin markedly reduces fibrosis-related indicators, and this process is partially achieved through the regulation of ERK1/2 phosphorylation.</div></div><div><h3>Conclusion</h3><div>In conclusion, this study reveals that arbutin exerts a significant protective effect by acting on the ERK1/2 signaling pathway, effectively inhibiting myocardial fibrosis, and subsequently attenuating myocardial infarction induced by LAD ligation.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102986"},"PeriodicalIF":2.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Codonopsis pilosula polysaccharides attenuate lipid accumulation and inflammatory response in the NAFLD mouse by regulating AMPK/ACC/SREBP1 signaling pathway","authors":"Qiu Chen ,&nbsp;Hai Lin ,&nbsp;Xianen Huang ,&nbsp;Weilai Yu","doi":"10.1016/j.tice.2025.102984","DOIUrl":"10.1016/j.tice.2025.102984","url":null,"abstract":"<div><div>This study investigated the effects of <em>Codonopsis pilosula</em> polysaccharides (CPP) on nonalcoholic fatty liver disease (NAFLD) induced by a high-fat diet in mice and its underlying mechanisms. Mice were divided into six groups: control, model, fenofibrate, and three CPP dosage groups (300, 200, 100 mg/kg). CPP reduced liver lipid accumulation, serum TC, TG, LDL-C, ALT, AST, MDA, IL-6, and TNF-α levels while increasing HDL-C, SOD, and GSH-Px levels (<em>P</em> &lt; 0.05). Histopathological analysis confirmed decreased lipid deposition. Western blot showed CPP enhanced AMPK and ACC phosphorylation, while RT-PCR revealed reduced expression of SREBP1 and its target genes. In PA-induced HepG2 cells, CPP effects were reversed by AMPK inhibition. These findings demonstrate CPP’s potential to mitigate hepatocellular lipotoxicity, oxidative stress, and inflammation in NAFLD through the AMPK/ACC/SREBP1 pathway, offering a promising therapeutic approach.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102984"},"PeriodicalIF":2.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promotion of skin regeneration in diabetic rats by collagen-based hydrogel incorporated with basic fibroblast growth factor: A histological, molecular, and tensiometrical study","authors":"Melody Omraninava ,&nbsp;Rafat Rezapour-Nasrabad ,&nbsp;Mojgan Hosseini ,&nbsp;Mohammad Armin Kasiri ,&nbsp;Shadman Shahzamani ,&nbsp;Maryam Bahrami ,&nbsp;Zahra Sadrzadeh-aghajani ,&nbsp;Mohamad Sedigh Mirzaie","doi":"10.1016/j.tice.2025.102983","DOIUrl":"10.1016/j.tice.2025.102983","url":null,"abstract":"<div><div>Diabetic wounds represent a major and costly challenge for diabetic patients, leading to significant morbidity and healthcare expenses. Consequently, extensive research has been dedicated to identifying effective treatments to enhance wound healing. Among these, biological hydrogels have emerged as promising candidates due to their superior properties over traditional materials. This study aimed to assess the efficacy of a bioactive and biodegradable collagen-based hydrogel derived from human amniotic membrane (CHA) combined with basic fibroblast growth factor (bFGF) in promoting wound healing in diabetic rats. A total of thirty diabetic rats were randomly divided into three groups (n = 10): control, CHA, and CHA incorporated with bFGF (CHA+bFGF). Wound evaluations were conducted on days 7 and 21. The findings revealed notable improvements in wound closure, fibroblast and blood vessel counts, collagen density, tensiometrical parameters, and the levels of VEGF in the treatment groups compared to the control group, with the most significant effects observed in the CHA+bFGF group. Moreover, the CHA+bFGF group demonstrated a greater reduction in inflammatory cells infiltration, along with lower concentration of TNF-α and IL-1β cytokines, compared to the other groups. In conclusion, the combination of CHA with bFGF proved to be highly effective in enhancing the healing process of diabetic wounds.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102983"},"PeriodicalIF":2.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of misoprostol in the prevention of amikacin-induced neuronal damage","authors":"Sebile Azırak ,&nbsp;İlkay Armağan","doi":"10.1016/j.tice.2025.102981","DOIUrl":"10.1016/j.tice.2025.102981","url":null,"abstract":"<div><div>Amikacin (AK) is an aminoglycoside widely used in the treatment of Gram-negative infections which are life-threatening. In the etiology of toxicity, AK has been shown to cause tissue damage through oxidative stress and apoptosis as a consequence of the reactive oxygen species (ROS) production. Misoprostol (MP) is a prostaglangin E1 (PGE1) analogue with antioxidant, antiapoptotic, cytoprotective properties used to prevent gastrointestinal disorders induced by nonsteroidal anti-inflammatory drug. This study aims to investigate the neuroprotective effects of MP on brain cells against the neurotoxicity of AK by gene expression and histopathological analyses. Twenty-four male Spraque-Dawley rats were randomly separated into four groups (group 1, control; group 2, AK; group 3, MP; group 4, AK + MP). According to our findings, AK treatment significantly increased brain weight, brain weight/body weight ratios, and CYP2B1 mRNA gene expression. This was accompanied by histopathological changes such as eosinophilic neurons, pyknotic nuclei, vacuolated neuropil, congestion and inflammation in blood vessels. On the other hand, while significant improvements were observed in brain weight and brain weight/body weight ratios in the AK + MP group, there was a significant decrease in CYP2B1 mRNA gene expression levels and histopathological changes. According to our results, MP may serve as a potential therapeutic candidate to reduce the severity of oxidative damage induced by AK in brain tissue.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102981"},"PeriodicalIF":2.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}