Bioengineered chitosan/silk scaffold encapsulated with quercetin nanoparticles accelerates wound healing in a diabetic rat skin defect model

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-02 DOI:10.1016/j.tice.2025.103119

Suad Hamdan Almasoudi

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引用次数: 0

Abstract

Chronic wounds, particularly in diabetic patients, are characterized by prolonged inflammation, impaired angiogenesis, and delayed tissue regeneration. To address these challenges, the author developed a bioactive scaffold by incorporating quercetin nanoparticles (Qn) into a chitosan/silk fibroin (ChS) matrix, aiming to accelerate and enhance the wound healing process. Quercetin nanoparticles were synthesized via a solvent displacement method and incorporated into a ChS scaffold using a blending and freeze-drying technique. The physicochemical properties of the scaffold, including porosity, swelling ratio, degradation profile, biocompatibility, mechanical properties, and drug release kinetics, were characterized. Full-thickness excisional wounds (20 mm) were created on the dorsum of streptozotocin-induced diabetic Wistar rats, which were divided into three groups (n = 5): untreated control, ChS, and Qn-loaded ChS (QnChS). Wound closure was monitored on days 7 and 14. Histological evaluations (H&E and Masson's Trichrome) assessed tissue regeneration, vascularization, and collagen deposition. Cytokine levels (bFGF, VEGF, TNF-α, IL-1β) were quantified using ELISA. The QnChS scaffolds displayed a porous structure with sustained drug release over 14 days and enhanced biodegradability. In vivo, the QnChS group showed significantly accelerated wound contraction, increased dermal thickness and epidermal length, and higher collagen deposition compared to the other groups (p < 0.05). Angiogenesis was notably improved, evidenced by a higher number of blood vessels and elevated VEGF levels. Additionally, QnChS scaffolds markedly reduced TNF-α and IL-1β while increasing bFGF expression, indicating enhanced resolution of inflammation and promotion of tissue regeneration. The Qn-loaded ChS scaffold effectively modulates the inflammatory response, promotes angiogenesis, and enhances dermal regeneration in diabetic wounds. These findings suggest its potential as a multifunctional wound dressing for managing chronic wounds in diabetic patients.

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槲皮素纳米颗粒包埋生物工程壳聚糖/丝支架促进糖尿病大鼠皮肤缺损模型创面愈合

慢性伤口，尤其是糖尿病患者，其特点是炎症持续，血管生成受损，组织再生延迟。为了解决这些问题，作者将槲皮素纳米颗粒（Qn）掺入壳聚糖/丝素（ChS）基质中，开发了一种生物活性支架，旨在加速和增强伤口愈合过程。采用溶剂置换法合成槲皮素纳米颗粒，并采用混合和冷冻干燥技术将其掺入ChS支架中。表征了支架的物理化学性质，包括孔隙率、溶胀率、降解谱、生物相容性、机械性能和药物释放动力学。在链脲唑素诱导的糖尿病Wistar大鼠背部制造全层切除创面（20 mm），将其分为3组（n = 5）：未处理对照组、ChS和qn加载组（QnChS）。在第7天和第14天监测伤口闭合情况。组织学评估（H&；E和马松三色）评估组织再生、血管形成和胶原沉积。ELISA法测定细胞因子（bFGF、VEGF、TNF-α、IL-1β）水平。QnChS支架具有多孔结构，药物持续释放超过14天，生物降解性增强。在体内，与其他组相比，QnChS组伤口收缩明显加快，真皮厚度和表皮长度增加，胶原沉积增多（p <； 0.05）。血管生成明显改善，血管数量增加，VEGF水平升高。此外，QnChS支架显著降低TNF-α和IL-1β，同时增加bFGF表达，表明增强炎症消退和促进组织再生。负载qn的ChS支架有效调节炎症反应，促进血管生成，并增强糖尿病伤口的皮肤再生。这些发现提示其作为一种治疗糖尿病患者慢性伤口的多功能伤口敷料的潜力。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.