蛋壳膜补充刺激血管生成和促进骨愈合的大鼠钻孔损伤模型

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-02 DOI:10.1016/j.tice.2025.103117

Nao Yashima , Wataru Minamizono , Hirai Suito , Takumi Okunuki , Kaoru Fujikawa , Shingo Nakai , Masafumi Ohsako

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引用次数: 0

摘要

发展新的辅助疗法来支持骨愈合仍然是一个重要的临床挑战。蛋壳膜（ESM）是一种从鸡蛋中提取的天然生物材料，近年来因其安全性、生物相容性和成本效益而备受关注。我们的目的是评估ESM补充对大鼠胫骨钻孔损伤模型骨愈合的影响。实验前驯化1周的7周龄雄性Wistar大鼠分为对照组、单纯骨损伤组和ESM骨损伤组（EM）。显微计算机断层扫描和组织学分析显示，EM组在钻孔损伤后第14天和第21天的新骨体积显著增加。第21天，甲苯胺蓝染色减少，骨细胞计数降低，胶原纤维成熟。Sp7 mRNA表达增加，骨钙素阳性细胞数量增加。Nfatc1的表达和抗酒石酸酸性磷酸酶阳性破骨细胞的数量也显著升高。观察期间，EM组血管面积和Vegfa表达均明显增加。这些发现表明，ESM补充剂通过刺激血管生成和增强成骨细胞和破骨细胞活性来促进骨愈合，为支持和加速骨折愈合提供了一种有前途的营养辅助剂。

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Eggshell membrane supplementation stimulates angiogenesis and promotes bone healing in a rat drill-hole injury model

The development of new adjunct therapies to support bone healing remains an important clinical challenge. Eggshell membrane (ESM), a natural biomaterial derived from chicken eggs, has recently attracted attention for its safety, biocompatibility, and cost-effectiveness. We aimed to evaluate the effects of ESM supplementation on bone healing in a rat tibial drill-hole injury model. Seven-week-old male Wistar rats, acclimated for one week prior to the experiment, were divided into three groups: control, bone injury only, and bone injury with ESM supplementation (EM). Micro-computed tomography and histological analyses revealed a significant increase in new bone volume in the EM group at days 14 and 21 post-drill hole injury. On day 21, reduced toluidine blue staining, a lower osteocyte count, and the maturation of collagen fibers. Moreover, Sp7 mRNA expression and the number of osteocalcin-positive cells were increased. The expression of Nfatc1 and the number of tartrate-resistant acid phosphatase-positive osteoclasts were also significantly elevated. In addition, the vascular area and Vegfa expression were markedly increased in the EM group throughout the observation period. These findings suggest that ESM supplementation promotes bone healing by stimulating angiogenesis and enhancing osteoblast and osteoclast activity, offering a promising nutritional adjunct to support and accelerate fracture healing.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.