改变肝脏微观结构的离体力学和显微结构评价

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-03 DOI:10.1016/j.tice.2025.103121

Alexander Chan Kai Foong , Claire Dempsey , Brooke Tornifoglio

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引用次数: 0

摘要

背景/目的近年来无创肝脏影像学的进展在疾病诊断和监测方面具有重要的潜力。然而，不同的微观结构特征对肝脏临床来源的机械生物标志物的影响尚未完全阐明。本研究旨在探讨肝组织内微结构变化对力学的影响。方法通过力学试验和组织学处理，评价酶处理对猪肝组织的影响，以及酶处理对猪肝组织微观结构的影响。结果我们发现，肝组织的保存方法对组织的压缩材料性能有显著影响，而对组织的拉伸材料性能没有影响。此外，我们发现通过胶原酶的酶处理改变了肝组织的微观结构和力学，在压缩方面也更为显著。结论本研究为进一步建立离体肝脏模型奠定了基础，以更好地了解肝脏微观结构的变化及其对力学的影响，最终提高我们对临床衍生生物标志物的理解。

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Ex vivo mechanical and microstructural evaluation of a changing hepatic microstructure

Background/objectives

Recent advancements in non-invasive imaging of the liver hold significant potential for disease diagnosis and monitoring. However, the influence of different microstructural features on clinically derived mechanical biomarkers in the liver are not fully elucidated. The aim of this study was to investigate the influence of microstructural changes within liver tissue on mechanics.

Methods

The impact of storage and preservation, as well as bulk microstructural changes via enzymatic treatments were evaluated by mechanical testing and histological processing on porcine liver.

Results

We found that the preservation method chosen for ex vivo liver tissue significantly influences the compressive material properties of the tissue, while not impacting those in tension. Additionally, we found that enzymatic treatments via collagenase alter the microstructure and mechanics, again more significantly in compression, of liver tissue.

Conclusion

This work lays foundational insights for future studies which aim to develop ex vivo liver models to better understand the changing microstructure in the liver and its influence on mechanics, ultimately improving our understanding of clinically derived biomarkers.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.