Hepatoprotective role of pumpkin seed oil in colchicine-treated rats through redox balance, apoptosis modulation, and anti-fibrotic effects

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-04 DOI:10.1016/j.tice.2025.103128

Yasmina M. Abd-Elhakim , Ezzat F.F. Soliman , Manar A. AbdelMageed , Azza M.A. Abo-Elmaaty , Taghred M. Saber , Nabela I. El-Sharkawy , Gihan G. Moustafa , Fathy Elsayed Abdelgawad , Engy Mohamed Mohamed Yassin

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引用次数: 0

Abstract

Colchicine (CNE) is used to treat various inflammatory conditions, but its use is limited by its hepatotoxic potential. This study assessed the protective effect of pumpkin seed oil (PPN) against CNE-induced liver injury in rats. Forty male Sprague-Dawley rats were allocated into four groups: control, PPN (4 mL/kg b.wt), CNE (0.6 mg/kg b.wt), and CNE+PPN, and received oral treatments for 56 days. CNE significantly elevated liver enzymes (ALP, GGT, AST, and ALT) and bilirubin levels, and induced dyslipidemia, while PPN co-treatment restored these parameters toward normal. Antioxidant defenses, including SOD, CAT, and GSH were significantly suppressed by CNE, accompanied by increased MDA, protein carbonyls, 8-OHdG, and caspase-3 activity. PPN treatment improved redox status and reduced apoptotic markers. Histologically, CNE caused ductular reactions, bile duct hyperplasia, and collagen fiber accumulation around the portal triads. Masson's trichrome staining confirmed significant fibrosis, which was significantly reduced with PPN co-administration. Immunohistochemistry revealed a significant reduction in PCNA in CNE-treated livers, partially restored by PPN. BAX expression was significantly increased by CNE and notably reduced in the CNE+PPN group. These findings highlight the hepatoprotective potential of PPN through antioxidant, anti-apoptotic, anti-fibrotic, and lipid-regulating mechanisms, supporting its use as a natural beneficial agent against CNE-induced liver injury.

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南瓜籽油通过氧化还原平衡、细胞凋亡调节和抗纤维化作用对秋水仙碱处理大鼠的肝保护作用

秋水仙碱（CNE）被用于治疗各种炎症，但由于其潜在的肝毒性，其使用受到限制。本研究评价南瓜籽油（PPN）对cne诱导大鼠肝损伤的保护作用。选取雄性sd大鼠40只，分为对照组、PPN组（4 mL/kg b.wt）、CNE组（0.6 mg/kg b.wt）、CNE+PPN组，口服治疗56 d。CNE显著升高肝酶（ALP、GGT、AST和ALT）和胆红素水平，诱导血脂异常，而PPN联合治疗使这些参数恢复正常。CNE显著抑制抗氧化防御，包括SOD、CAT和GSH，同时增加MDA、蛋白羰基、8-OHdG和caspase-3活性。PPN处理改善了氧化还原状态，减少了凋亡标志物。组织学上，CNE引起导管反应、胆管增生和胶原纤维在门静脉三联静脉周围积聚。马松三色染色证实有明显的纤维化，PPN联合给药明显减轻了纤维化。免疫组织化学显示，cne处理的肝脏中PCNA显著减少，PPN部分恢复。CNE组BAX表达明显升高，而CNE+PPN组BAX表达明显降低。这些发现强调了PPN通过抗氧化、抗凋亡、抗纤维化和脂质调节机制的肝保护潜力，支持其作为天然有益剂用于抗cne诱导的肝损伤。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.