Tissue & cell最新文献

{"title":"BDNF alleviates senescence and enhances osteogenic differentiation in bone marrow mesenchymal stem cells via the TrkB/PI3K/AKT pathway","authors":"Jimei Zhang ,&nbsp;Ling Zhu ,&nbsp;Jianping Zhou ,&nbsp;Qunying Yu ,&nbsp;Guangyuan Yang ,&nbsp;Chaoli Luo ,&nbsp;Jianguo Meng ,&nbsp;Shan Xing ,&nbsp;Jing Liu ,&nbsp;Donggang Mou ,&nbsp;Xuming Yang","doi":"10.1016/j.tice.2025.102972","DOIUrl":"10.1016/j.tice.2025.102972","url":null,"abstract":"<div><h3>Background</h3><div>Bone marrow mesenchymal stem cells (BMSCs) are stem cells that reside in bone marrow and have multidirectional differentiation potential. BMSCs have been used to treat bone injury. However, long-term passage leads to the aging of BMSCs and the weakening of osteogenic differentiation. Furthermore, brain-derived neurotrophic factor (BDNF) may enhance the antiaging ability of BMSCs. The purpose of this study was to investigate the role of BDNF in the senescence and osteogenic differentiation of human BMSCs (hBMSCs).</div></div><div><h3>Methods</h3><div>The senescence of hBMSCs was induced by successive passages. The mRNA and protein expression levels were measured using RT<img>qPCR and Western blotting. Alkaline phosphatase (ALP) and alizarin red S (ARS) staining were used to identify osteogenic differentiation in the cells.</div></div><div><h3>Results</h3><div>After long-term passage, the hBMSCs morphologically gradually expanded and appeared flat, cell viability decreased, the number of fibroblast-like colony-forming units (CFU-Fs) decreased, and the number of β-galactosidase (SA-β-gal)-positive cells and the mRNA expression levels of the senescence-related genes p53, p21 and p16 increased. The activity of ALP, the level of calcium salt deposition and the protein levels of runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN) and BDNF were significantly decreased. Subsequent research indicated that the senescence and inhibition of the osteogenic differentiation of hBMSCs induced by long-term culture were caused by low expression of BDNF. From a mechanistic standpoint, BDNF can activate the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway by upregulating the expression of tropomyosin receptor kinase B (TrkB), thereby improving the senescence and inhibition of the osteogenic differentiation of hBMSCs caused by long-term passage.</div></div><div><h3>Conclusion</h3><div>BDNF improves the senescence and inhibition of the osteogenic differentiation of hBMSCs caused by long-term passage via regulation of the TrkB/PI3K/AKT signaling axis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102972"},"PeriodicalIF":2.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting the p38MAPK/STAT3/NF-κB65 pathway modulates the hepatoprotective effect of pentoxifylline against sodium valproate-induced liver injury in rats; A study based on integrating network pharmacology and experiment validation","authors":"Gellan Alaa Mohamed Kamel ,&nbsp;Shaimaa Hussein","doi":"10.1016/j.tice.2025.102942","DOIUrl":"10.1016/j.tice.2025.102942","url":null,"abstract":"<div><div>Sodium valproate (SVP) is frequently utilized in the treatment of epilepsy. Extended administration of SVP elevates the likelihood of hepatotoxicity. Pentoxifylline (PTX) functions as a nonselective phosphodiesterase inhibitor and is commonly utilized in the treatment of vascular conditions, exhibiting extensive antioxidant and anti-inflammatory properties. This research examined PTX's protective role in mitigating SVP-induced liver injury, clarifying the potential molecular mechanisms involved. SVP and PTX intersection targets were identified through network pharmacology. In addition, we conducted an in vivo experiment utilizing the SVP-induced liver injury model. Eighteen adult male rats were allocated to three groups, with the control group receiving oral normal saline. The SVP group was administered SVP (dissolved in normal saline) daily for 2 weeks. The PTX + SVP group was administered SVP in conjunction with PTX daily for two weeks. PTX hepatoprotective effect against SVP could include apoptosis, anti-inflammatory mechanisms, and various signaling pathways, such as NF-κB, STAT3, and MAPK, as indicated by the KEGG and GO enrichment analysis of the 272 common targets. We identified BCL2, IL6, and TNF as potential hub targets of PTX for liver injury treatment based on the construction of the PPI network. In vivo experiments demonstrated that PTX hindered MAPK P38/STAT3/NF-κB-related pathways' activation, diminished oxidative stress, apoptosis, and inflammatory response, and substantially ameliorated SVP-induced liver injury. The network pharmacology study concluded that the MAPK P38/STAT3/NF-κB pathway plays a role in PTX protective effect against SVP-induced liver injuries, as demonstrated by the <em>in vivo</em> experiment results.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102942"},"PeriodicalIF":2.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vascular embolic nanobiomaterials for efficient tumor treatment","authors":"Jihyuk Yang ,&nbsp;Yonghyun Choi ,&nbsp;Suyeon Ahn ,&nbsp;Heejin Ha ,&nbsp;Jiwon Kim ,&nbsp;Jaehee Jang ,&nbsp;Masayoshi Tanaka ,&nbsp;Hee-Young Lee ,&nbsp;Jonghoon Choi","doi":"10.1016/j.tice.2025.102954","DOIUrl":"10.1016/j.tice.2025.102954","url":null,"abstract":"<div><div>Embolization is a minimally invasive cancer treatment method. Embolization involves artificially blocking blood flow using an embolic agent to block abnormal blood vessels that supply nutrients or oxygen to a specific lesion, thereby killing the lesion, inhibiting its growth, and stopping bleeding. Currently, polyvinyl alcohol (PVA) and gelatin are the most popular embolic agents. These substances are available in various sizes and shapes that physically obstruct blood flow to cause vascular embolization. They are commonly used due to their ease of use and low cost. However, they can cause side-effect such as bleeding and potential complications related to catheter- and insertion-related complications. Recently, nanobiomaterials have been explored as embolization agents with high biocompatibility, such as liquid metals, and can be used with autologous blood. In this review, we cover the types of embolic agents currently used in cancer treatment and focus on those with fewer adverse effects and minimal vascular damage, followed by discussions on new embolic agents under development. Additionally, we explore potential future research directions for developing better embolic agents.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102954"},"PeriodicalIF":2.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colcemid duration effects on extrachromosomal DNA analysis in breast cancer cell line","authors":"Shadira Anindieta Irdianto ,&nbsp;Fadhillah Fadhillah ,&nbsp;Retno Lestari ,&nbsp;Fadilah Fadilah ,&nbsp;Astari Dwiranti ,&nbsp;Anom Bowolaksono","doi":"10.1016/j.tice.2025.102952","DOIUrl":"10.1016/j.tice.2025.102952","url":null,"abstract":"<div><div>Extrachromosomal DNA (ecDNA) is a circular form of genetic material that exists outside the chromosomes and plays a significant role in tumour heterogeneity and drug resistance. In this preliminary study, we aimed to optimize the visualization of ecDNA by investigating the effects of varying colcemid treatment durations on metaphase arrest in MDA-MB-231 breast cancer cells. We tested four durations of colcemid treatment: 20 hours, 3 hours, 1 hour, and 30 minutes. Using light microscopy, confocal microscopy, and scanning electron microscopy, we analyzed the morphology and presence of ecDNA. Our results demonstrated that 20-hour colcemid treatment yielded the most well-spread metaphase cells, enabling the optimal observation of ecDNA. These structures appeared as ring-like formations with lower DAPI intensity than chromosomes and were often found in pairs. While our findings contribute valuable insights into the visualization of ecDNA, they also serve as a foundation for future research.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102952"},"PeriodicalIF":2.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological aspect of the jaw system of meagre (Argyrosomus regius): New insights into jaw teeth distribution, characterization, and their relationship to its carnivorous behaviour","authors":"Mohamed M.A. Abumandour,&nbsp;Basma G. Hanafy","doi":"10.1016/j.tice.2025.102944","DOIUrl":"10.1016/j.tice.2025.102944","url":null,"abstract":"<div><div>Documentation on the morphological appearance of teeth and their relationship to carnivorous feeding habits was lacking. Our study was applied to twelve <em>Argyrosomus regius</em> using the gross scanning electron microscopic techniques to provide a detailed ultrastructural description of the jaw, its tooth arrangement, and the relationship to carnivorous feeding behavior, which had not been previously described. The upper velum's oral surface contained only taste buds. There were two teeth in the upper caudal premaxillary fangs and four in the lower. Two premaxillary teeth groups were identified: the median upper of 3–4 rows and the median lower of 4–5 rows. The lateral upper incisive group had numerous small teeth (in 3–4 rows) and the long ones in one row, while the lateral lower one was organized in two rows: large posterior and small anterior. The dental arrangement in the lateral upper premaxillary group is highly specialized, with numerous small teeth in three or four rows and long teeth in one row. In contrast, the lateral lower group exhibited a different pattern, with small, short, straight teeth arranged in groups parallel to the dental space. The upper lateral tooth band had two types: the medial lower one of long teeth, while the medial upper one of small, short teeth in four or five rows, except anteriorly in just two rows. Conclusion, the arrangement and size of teeth in fish's upper and lower jaws can reveal their diet and feeding habits, and studying tooth morphology can aid researchers in understanding their evolutionary adaptations.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102944"},"PeriodicalIF":2.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of dual-specificity phosphatase 3 drives differentiation and polarization of myeloid leukemia cells into macrophages with reduced proliferative and DNA repair fitness","authors":"Jessica O. Farias,&nbsp;Diana R.D.C.G. Pacheco,&nbsp;Yuli T. Magalhaes,&nbsp;Lilian C. Russo,&nbsp;Viktor K. Boell,&nbsp;Donna J.F. Hilares,&nbsp;Fabio L. Forti","doi":"10.1016/j.tice.2025.102947","DOIUrl":"10.1016/j.tice.2025.102947","url":null,"abstract":"<div><div>Dual-specificity phosphatase 3 (DUSP3) regulates key cellular processes, including the cell cycle, proliferation, and differentiation. Recently, we demonstrated its crucial role in maintaining genomic stability by interacting with and dephosphorylating nucleophosmin (NPM), thereby modulating nuclear p53 activity under genotoxic stress. Given the frequent mutations in both p53 and NPM in acute myeloid leukemia (AML), this study aimed to investigate the impact of DUSP3 knockdown in two p53-deficient AML cell lines and explore potential correlations with NPM expression. THP-1 cells exhibited higher basal levels of DUSP3 and NPM compared to HL-60 cells, while DUSP3 knockdown reduced NPM expression in HL-60 cells. Upon phorbol 12-myristate 13-acetate (PMA)-induced differentiation into macrophage-like cells, only HL-60 cells displayed decreased levels of both DUSP3 and NPM. DUSP3 knockdown enhanced differentiation in THP-1 and HL-60 cells and promoted non-classical M2 macrophage polarization following additional PMA exposure, as indicated by increased expression of CD11b and CD206. Bioinformatics analysis revealed significant correlations between DUSP3 and NPM gene expression, AML patient survival, and the maturation stage of myeloid cells. Furthermore, DUSP3 knockdown in undifferentiated HL-60 cells impaired proliferation and compromised genomic stability under genotoxic stress induced by doxorubicin. These findings suggest that DUSP3 plays a regulatory role in the differentiation, polarization, and proliferation of myeloid cells. Through the modulation of NPM expression and activity, DUSP3 may contribute to a deeper understanding of leukemia pathophysiology and mechanisms of chemotherapy resistance.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102947"},"PeriodicalIF":2.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a biologically-inspired airway epithelial model using a bronchial decellularized scaffold: Applicability for pulmonary preclinical studies","authors":"Artur Christian Garcia da Silva,&nbsp;Izadora Caroline Furtado de Mendonça,&nbsp;Sérgio de Morais Carvalho-Filho,&nbsp;Tatyane Gonçalves Hayasaki,&nbsp;Marize Campos Valadares","doi":"10.1016/j.tice.2025.102943","DOIUrl":"10.1016/j.tice.2025.102943","url":null,"abstract":"<div><div>The search for physiologically relevant 3D airway models <em>in vitro</em> has made significant progress in recent decades. The goal has been to replace animal-based methods with assays that mimic the human response to toxicants exposure through inhalation. However, replicating the entire composition of the extracellular matrix (ECM) microenvironment has proven challenging. Some strategies, such as 3D bioprinting and commercial hydrogels and biomatrixes, have addressed this issue. Yet, it remains difficult to recreate tissue topography, the location of anchorage proteins, and the exact protein and non-protein composition of ECM. In this study, we aimed to obtain and characterize a low-cost decellularized bronchial porcine scaffold derived from food industry waste. This scaffold was intended for the reconstruction of 3D bronchial models using human cells for long-term cultivation. Fragments from the main right and left bronchi underwent various matrix decellularization methods, including surfactant solutions, osmotic gradient, and nuclease treatment. The results showed that all these approaches efficiently promoted cell removal while preserving collagen content, glycosaminoglycans, and the basal membrane. Next, we repopulated the decellularized ECM (dECM) with Calu-3 epithelial cells and cultivated them in an air-liquid interface (ALI) to assess cell behavior within the scaffold. Over 7 and 14 days of ALI cultivation, the cells exhibited progressive growth on the bronchial dECM, expressing regular pan-cytokeratin, MUC1, and E-cadherin. In summary, we successfully developed a low-cost, biologically relevant dECM that was employed for reconstructing human airway models. These models hold promises for use in preclinical respiratory research studies.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102943"},"PeriodicalIF":2.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of yeast-mediated SeNPs on gastric ulcer healing and gut microbiota dysbiosis in male albino rats","authors":"Aya Adel Fath-All ,&nbsp;Tarek Atia ,&nbsp;Ayman Saber Mohamed ,&nbsp;Neveen M. Khalil ,&nbsp;Tamer D. Abdelaziz ,&nbsp;Neamat A. Mahmoud ,&nbsp;Abdallah Mohammed Elagali ,&nbsp;Hader I. Sakr ,&nbsp;Mohamed N. Abd El-Ghany","doi":"10.1016/j.tice.2025.102953","DOIUrl":"10.1016/j.tice.2025.102953","url":null,"abstract":"<div><h3>Background</h3><div>Gastric ulcer is one of the most common gastrointestinal tract diseases with a higher extent in male patients to. Selenium nanoparticles (SeNPs) possess therapeutic benefits, including antimicrobial, antioxidant, anti-inflammatory, and anti-ulcerative agents. The study aimed to investigate the modulatory effect of yeast-mediated SeNPs on gastric ulcers and microbiota dysbiosis in a rat model.</div></div><div><h3>Method</h3><div>Twenty-four rats were randomly divided into four groups. Both the control and SeNPs-only groups received distilled water orally, and after 1 h, they received 2 % carboxymethyl cellulose (CMC). The ulcer model and SeNPs-treated groups received 99 % ethanol (5 ml/kg orally) for ulcer induction, followed by 2 % CMC after one hour. The SeNPs-treated group got SeNPs (60 mg/kg) suspended in 2 % CMC. We measured ulcer markers (ulcer index and gastric juice pH and volume and stomach tissue oxidative stress markers (malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO), and catalase (CAT)), in addition to histopathological examination of gastric tissues stained with three different satins: hematoxylin-eosin (H&amp;E), periodic acid-Schiff (PAS), and Masson's trichrome stains (many-color dye), and microbiological analysis of freshly collected fecal sample.</div></div><div><h3>Results</h3><div>SeNPs treatment significantly decreased gastric volume, ulcer index, malondialdehyde, and increased glutathione levels. A macroscopic examination of the treated stomach revealed decreased ulcer lesion numbers. Furthermore, histopathological examination showed that SeNPs treatment repaired ulcerative gastric tissue through the regeneration of epithelial cells and reduction in damaged areas and collagen fibers. In the treated group, microbiological analysis of rat feces showed a significant increase in Leuconostoc pseudomesenteroides, Escherichia coli, and Enterococcus faecium counts.</div></div><div><h3>Conclusion</h3><div>This research suggests that SeNPs exhibit anti-ulcer activity and can accelerate ulcer healing via their antioxidant action. They also have a modulatory effect on gut microbiota dysbiosis associated with gastric ulcers. This is the first research studying the impact of safe yeast-mediated SeNPs on rat's gastric ulcer and gut microbiota.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102953"},"PeriodicalIF":2.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Could hesperetin ameliorate doxorubicin-induced nephrotoxicity in rats via its antioxidant, antiapoptotic, and anti-inflammatory properties?","authors":"Rabab Ahmed Rasheed ,&nbsp;A.S. Sadek ,&nbsp;R.T. Khattab ,&nbsp;Diana Z. Saad ,&nbsp;Noha O. Shawky ,&nbsp;Heba A. Abdelfattah","doi":"10.1016/j.tice.2025.102951","DOIUrl":"10.1016/j.tice.2025.102951","url":null,"abstract":"<div><div>Doxorubicin (DOX), from the anthracycline family, is a widely utilized chemotherapy for various malignancies; however, its utility is limited due to the serious adverse reactions, particularly on the kidneys, primarily related to oxidative stress, inflammation, and apoptosis. Hesperetin (HES), the citrus fruit derivative, is a naturally occurring flavonoid. Previous studies underscored HES’s protective efficacy against renal damage in several disorders in rodents through its proven antioxidant, antiapoptotic, and anti-inflammatory properties. This work explored the protecting role of HES against the nephrotoxic effects of DOX and the possible underlying mechanisms. Nephrotoxicity was induced in rats via administering six equal doses of DOX (3 mg/kg/week, i.p) for six consecutive weeks. The treated group received HES (50 mg/kg/day, p.o.) simultaneously with DOX. Rats’ body and kidney weights, serum creatinine, blood urea nitrogen (BUN), and albumin were estimated. Kidney tissue was treated to assess redox status, histopathological, and immunohistochemical alterations. Compared to the controls, coadministration of HES with DOX significantly reduced the serum BUN and creatinine, elevated the serum albumin, amended the glomerular distortion and tubular epithelial degeneration, decreased collagen deposition, vascular congestion, and inflammatory cells in addition to the significant attenuation of inflammatory cytokines and proapoptotic markers. Our study is the first of its kind to underscore the HES’s antioxidant, antiapoptotic, and anti-inflammatory activities in an experimental model of DOX-induced nephrotoxicity with emphasis on TNF-α, IL-1β, and IL-6 signaling pathway, rendering it as an effective therapeutic supplement that could alleviate the nephrotoxic effect of DOX.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102951"},"PeriodicalIF":2.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunolabel imaging of plasma membrane Ca++ pumps (PMCA-pan or PMCA2) implicates the apical cilium on receptor cells as the transduction site for electrosense in two Acipenseriformes fishes","authors":"David F. Russell ,&nbsp;Mehmood Karimi ,&nbsp;Lilia L. Neiman","doi":"10.1016/j.tice.2025.102928","DOIUrl":"10.1016/j.tice.2025.102928","url":null,"abstract":"<div><div>Specialized receptor cells in ampulla of Lorenzini-like electroreceptors enable Acipenseriformes fishes to detect microvolt signals from aquatic prey. Candidate subcellular sites of electrosensory transduction include each receptor cell’s apical face membrane, or its modified primary cilium, or sparse apical microvilli. We aimed to more precisely localize the apical transduction. Given its reported Ca⁺⁺ dependence, as proxy we immunolabeled electrosensory epithelia for Ca⁺⁺ ion pumps (PMCA: plasma membrane calcium ATPase), in frozen sections of ampullary organs from rostrum skin of freshwater paddlefish (<em>Polyodon spathula</em>) and shovelnose sturgeon (<em>Scaphirhynchus platorynchus</em>). We trialed two anti-PMCA antibodies: pan clone 5F10, or polyclonal anti-PMCA2. Most somatic plasma membrane of receptor cells was diffusely 5F10⁺, with dispersed bright punctate domains. Both anti-PMCA antibodies labeled the apical cilium on receptor cells, along most cilium length to a conical tip. Patterning of ciliary PMCA⁺ label varied: it was continuous on some shafts, or showed regional gradation with cilium length, or shafts could be banded or helically striped at 0.5–1 micron helix pitch. PMCA⁺ cilia had consistent cylindrical shape and dimensions in paddlefish: 5.6 micron total length, and 0.7–1 micron shaft OD (∼3x wider than other vertebrate primary cilia). By contrast, the apical face membrane of receptor cells expressed faint or nil 5F10⁺ label, imaged as a dark annulus encircled by zona occludens (anti-ZO1⁺). Microvilli were often absent apically. Marked expression of PMCAs on the apical cilium of receptor cells indicated Ca-dependent signaling there, consistent with ciliary transduction for electrosense in Acipenseriformes fishes.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102928"},"PeriodicalIF":2.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}