Tissue & cell最新文献

{"title":"The antioxidant and anticonvulsant effects of ellagic acid in kainic acid-induced temporal lobe epilepsy in mice","authors":"Ali Nesari ,&nbsp;Erfan Mardani ,&nbsp;Mehdi Goudarzi ,&nbsp;Susan Sabbagh ,&nbsp;Mohammadreza Rashidi Nooshabadi ,&nbsp;Nima Bakhtiari ,&nbsp;Ali Reza Malayeri","doi":"10.1016/j.tice.2025.102889","DOIUrl":"10.1016/j.tice.2025.102889","url":null,"abstract":"<div><div>Oxidative stress (OS) resulting from high levels of free radicals contributes to the initiation and progression of epilepsy. Temporal lobe epilepsy (TLE) is associated with alteration in the structure and function of the hippocampus and is modeled in mice using kainic acid (KA). In this study, the neuroprotective effect of ellagic acid (EA) on KA-induced epilepsy in mice was evaluated. Sixty male Swiss albino mice were assigned to six groups: I received normal saline (NS; 10 ml/kg, intraperitoneally (i.p.)); II, received KA (15 mg/kg, i.p.); III, received diazepam (20 mg/kg, i.p.) and KA (15 mg/kg, i.p.); IV-VI, received EA (10, 20 and 40 mg/kg, i.p.), and KA (15 mg/kg, i.p.). Treatments were done 30 min before KA injection. Seizure (latency, duration, activity) and mortality were monitored for 2 h post-injection. OS was evaluated by measuring MDA, NO, and GSH levels and CAT, SOD, and GPx activities. Levels of TNF-α in the brain tissue were measured. Furthermore, a histological examination of the hippocampus was carried out.</div></div><div><h3>Results</h3><div>showed that EA pretreatment caused a decline in seizure activity score and duration compared to the KA-treated group. EA pretreatment reduced mortality in KA-treated mice. EA suppressed the generation of MDA and NO; whereas it preserved GSH and the activity of GPx, SOD, and CAT. Additionally, EA exerted anti-inflammatory effects by reducing TNF-α level. Histopathologically, EA reduced KA-induced neuronal damage. EA demonstrated protective effects against KA-related epilepsy and brain damage. Due to its anti-inflammatory and radical scavenging properties, EA may be considered a potential therapeutic option in epilepsy.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102889"},"PeriodicalIF":2.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bavachinin attenuates cerebral ischemia/reperfusion injury in rats via its anti-inflammatory and antioxidant effects","authors":"Amirhossein Atlasbaf ,&nbsp;Armin Hashem Kodehei ,&nbsp;Ensiyeh Bahadoran ,&nbsp;Isareza Zare ,&nbsp;Salar Yousefzadeh ,&nbsp;Prashant Kesharwani ,&nbsp;Yazdan Naderi ,&nbsp;Amirhossein Sahebkar","doi":"10.1016/j.tice.2025.102886","DOIUrl":"10.1016/j.tice.2025.102886","url":null,"abstract":"<div><div>Cerebral ischemia is associated with memory deficits. Bavachinin, a natural flavonoid derived from Psoralea Corylifolia seeds, exhibits various pharmacological properties, including anti-inflammatory, antioxidant, anticancer, and anti-allergic activities. We looked into the neuroprotective effects of bavachinin on rats' memory impairments brought on by transient cerebral ischemia/reperfusion. Blocked carotid arteries caused transient cerebral ischemia/reperfusion injury. Wistar male rats were randomized to bavachinin, ischemia/reperfusion, and sham groups. After surgery, bavachinin (100 mg/kg) was injected intraperitoneally once a day for seven days. Spatial memory was evaluated using the Morris water maze test. The vitality of the hippocampal pyramidal neurons was assessed by Nissl staining. The production of pro-inflammatory cytokines (TNF-α and IL-1β) has been detected using ELISA. Oxidative stress was evaluated by determining the hippocampal levels of malondialdehyde (MDA). In rats with transient cerebral ischemia/reperfusion, bevacichinin markedly improved the performance of learning. The bavachinin-treated group had a higher number of surviving pyramidal neurons, as demonstrated by the Nissl staining. In the hippocampus, bevacichinin lowered MDA, TNF-α, and IL-1β levels. The antioxidant and anti-inflammatory properties of bavachinin likely protect against memory impairment caused by transient cerebral ischemia. These findings suggest that bavachinin could be a potent therapeutic agent for the prevention of cognitive deficits and neuronal damage associated with cerebral ischemia/reperfusion. More studies are needed to explore its clinical applications and mechanisms of action.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102886"},"PeriodicalIF":2.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MMP-9 Expression in bone cells in the developing femur","authors":"Fatma El-Zahraa A. Mustafa ,&nbsp;Hanan H. Abd-Elhafeez ,&nbsp;Reda S. taha ,&nbsp;Amira Osman ,&nbsp;Mohamed M. Almaki ,&nbsp;Zyad Mohamed Baker ,&nbsp;Abdullah A.A. Alghamdi ,&nbsp;Diaa Massoud ,&nbsp;Mohamad Elmasry ,&nbsp;Soha A. Soliman ,&nbsp;Abeer Madkour Mahmoud","doi":"10.1016/j.tice.2025.102862","DOIUrl":"10.1016/j.tice.2025.102862","url":null,"abstract":"<div><div>The proteolytic functions of the matrix-degrading enzymes, such as ADAMTS (A disintegrin and metalloproteinases) and MMP (Matrix metalloproteinase), play a crucial role in development. This study investigated the proteolytic activity of different types of bone cells, focusing on the expression of MMP-9, throughout the development of the femur in rabbit embryos. The femur formed the physeal growth cartilage, which facilitated the process of endochondral bone development. Osteoblasts are situated on the outer layer of bone spicules and within the periosteum. Osteoclasts are found within the lacunae of the bone matrix, and they have also been observed in the ossification center. Osteoclasts, osteocytes, and osteoblasts all exhibit MMP-9 expression with different intensity of staining with the perichondral stem cells showing the greatest value. MMP-9 is essential in bone formation. Utilizing MMP-9 to modify the extracellular matrix could potentially serve as a favorable approach for identifying treatment targets in bone diseases.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102862"},"PeriodicalIF":2.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and ultrastructure study of lung tissue alterations in adult male albino rats exposed to methyl methacrylate vapor","authors":"Noha Gaber,&nbsp;Engy Adel Khalifa,&nbsp;Sara Shawky","doi":"10.1016/j.tice.2025.102864","DOIUrl":"10.1016/j.tice.2025.102864","url":null,"abstract":"<div><h3>Background</h3><div>Methyl methacrylate (MMA) is a respiratory irritant that has been.associated with occupational exposure. This study aimed to predict the potential effect.in lung tissue in response to MMA inhalation in mice.Aim: to detect the potential hazards associated with MMA inhalation in a rat model.Material and Methods: Adult male Wister albino rats were divided randomly into;Group I (control group); exposed to normal environmental condition for 4 weeks. Group.II (MMA group); exposed to MMA vapor at a concentration of 150 ppm 6 h/day, 5.days/week for 4 weeks. Collected lung specimens were examined by light and electron.microscopy.</div></div><div><h3>Results</h3><div>Collapse of the lung alveoli with thickening of the interalveolar septa were.detected in MMA group. Ultrastructure examination revealed irregular pneumocytes,prominent fibroblast with collagen fibers precipitate.Conclusion: chronic exposure to MMA may result in alveolar collapse and lung.fibrosis.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102864"},"PeriodicalIF":2.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rk1 inhibits the malignant progression of lung cancer by inactivating the INSR/PI3K/AKT pathway","authors":"Ning Du ,&nbsp;Dingli Song ,&nbsp;Xin Sun ,&nbsp;Hong Ren ,&nbsp;Yunfeng Zhang","doi":"10.1016/j.tice.2025.102880","DOIUrl":"10.1016/j.tice.2025.102880","url":null,"abstract":"<div><h3>Background</h3><div>Lung cancer continues to be a primary contributor to global cancer deaths. The urgent need for effective therapeutic interventions has led researchers to explore natural compounds with potential anti-cancer properties. Ginsenoside Rk1 (Rk1), a pharmacologically active component derived from ginseng, has garnered attention for its reported anti-cancer effects, however, the molecular mechanisms underlying its action against lung cancer remain poorly elucidated.</div></div><div><h3>Methods</h3><div>This study employed a series of <em>in vitro</em> assays, including cell counting kit-8, cell colony formation, 5-Ethynyl-2’-deoxyuridine, flow cytometry, and Transwell assays, to assess the effects of Rk1 on lung cancer cell viability, proliferation, apoptosis, migration, and invasion. Western blotting, immunohistochemistry assay, and quantitative real-time polymerase chain reaction were used to evaluate the protein and mRNA expression, respectively. Bioinformatics tools such as Genecards, Swiss Target Prediction, and Cytoscape 3.9.1 were utilized for network pharmacological analysis and pathway mapping. The interaction between Rk1 and INSR was analyzed using a cellular thermal shift assay. Animal experiment was performed to validate the effect of Rk1 and INSR overexpression on the malignant progression of lung cancer cells.</div></div><div><h3>Results</h3><div>Rk1 treatment significantly inhibited lung cancer cell proliferation, migration, and invasion, while inducing cell apoptosis and autophagy. Importantly, we identified insulin receptor (INSR) as a pivotal target of Rk1. Notably, INSR expression was found to be upregulated in lung cancer tissues and cells. In addition, Rk1 treatment heat-stabilized INSR and reduced its protein expression in A549 cells. Depletion of INSR mimicked the inhibitory effects of Rk1, whereas overexpression of INSR mitigated these effects. Furthermore, Rk1 inactivated the PI3K/AKT pathway through the regulation of INSR. Further, INSR overexpression attenuated Rk1 treatment-induced inhibitory effect on the malignant progression of lung cancer cells.</div></div><div><h3>Conclusion</h3><div>This study provided novel insights into the molecular mechanisms by which Rk1 exerted its anti-cancer effects in lung cancer, specifically through targeting the INSR/PI3K/AKT pathway. These findings not only contributed to the understanding of Rk1’s therapeutic potential but also offered a rationale for its further development as a promising therapeutic agent for the treatment of lung cancer. The identification of INSR as a key target represents a significant advancement in the field, highlighting the importance of natural compounds in modern cancer therapy.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102880"},"PeriodicalIF":2.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human lymphocytes chromosome structure in the presence of magnesium ions","authors":"Astari Dwiranti ,&nbsp;Kinanti Ayang Putri ,&nbsp;Davita Adryanti Felicia Sampe ,&nbsp;Shadira Anindieta Irdianto ,&nbsp;Raden Hannie Dewi Hadyani Kartapradja ,&nbsp;Anom Bowolaksono ,&nbsp;Andi Salamah ,&nbsp;Abinawanto","doi":"10.1016/j.tice.2025.102895","DOIUrl":"10.1016/j.tice.2025.102895","url":null,"abstract":"<div><div>The magnesium ion is one of the divalent cations that have an important role in chromosome condensation. Research about the role of Mg^2 + has been conducted on the chromosomes from various types of cells, especially human cancer cell lines. Nonetheless, no research has been conducted on normal human cells, such as blood cells and lymphocytes. Therefore, a study to evaluate the effect of Mg²⁺ on chromosome structure in normal cells is required. This study aims to assess the effects of Mg²⁺ on chromosome structure in lymphocytes using GTL-banding and karyotyping. Metaphase chromosomes were obtained by culturing blood cells, followed by banding using Giemsa and Leishman stain. Mg²⁺ role was assessed using XBE5 solution (5 mM Mg²⁺) as control, XBE solution (0 mM Mg²⁺), and 1 mM EDTA solution as a cation chelator. The chromosome value from each treatment was evaluated using Quality Assessment from the Association for Clinical Cytogenetics according to the International System for Human Cytogenomics Nomenclature (ISCN). The result shows that the control chromosome had a compact structure with a clear banding pattern and bold band intensity, while those treated with XBE and EDTA had a less compact and fibrous structure with an unclear banding pattern and thin band intensity. In addition, the average chromosome score±SD of the XBE (4.389 ± 0.607) and EDTA-treated (4.611 ± 0.607) chromosomes were higher than those of the control (4.222 ± 0.427). These data indicated that Mg²⁺ is essential in maintaining the structure of the human lymphocyte chromosome.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102895"},"PeriodicalIF":2.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pretreatment of stem cells from human exfoliated deciduous teeth with low dosage of chrysin induces autophagy and cell survival as a target in regenerative therapy","authors":"Xi Chen ,&nbsp;XiaoLiang Luo ,&nbsp;Jian Qin ,&nbsp;HongHui Xie ,&nbsp;Hui Xie","doi":"10.1016/j.tice.2025.102890","DOIUrl":"10.1016/j.tice.2025.102890","url":null,"abstract":"<div><h3>Background</h3><div>Stem cell-based therapies, particularly stem cells from human exfoliated deciduous teeth (SHED), are gaining attention for regenerative medicine. Autophagy plays a crucial role in stem cell function by maintaining cellular homeostasis. Understanding how agents like chrysin induce autophagy in SHED cells could improve stem cell maintenance, enhance functionality, and delay aging, thus offering new therapeutic avenues for stem cell-based regenerative treatments.</div></div><div><h3>Methods</h3><div>Flow cytometry as well as multi-lineage differentiation was performed to characterization of SHED cells. The MTT assay was conducted to determine the optimal concentration of chrysin. The expression of autophagy-related proteins, including LC3-II, p62, and Beclin-1, was analyzed using western blotting. To evaluate cell survival and regenerative capacity, the expression of TERT protein was examined. We further assessed the formation of autophagic vesicles using flow cytometry.</div></div><div><h3>Results</h3><div>SHED cells were positive for CD44 and CD73, while negative for CD31 and CD34. Differentiation assays demonstrate the ability of SHED cells to differentiate into osteoblasts and adipocytes. The MTT indicate an optimal concentration of 20 μM chrysin, potentially showing enhanced cell proliferation. Western blot analysis revealed increased expression of LC3-II and Beclin-1, as autophagy-related protein following chrysin treatment, while a significant decrease was observed in the level of p62 (****<em>P</em> &lt; 0.0001). TERT levels showed a slight decrease in treated group. Chrysin significantly increased the autophagic vesicles, as evidenced by thr percentage of MDC-positive cells (<em>**P</em> &lt; 0.001). Chrysin also induced slight apoptosis in SHED cells(<em>*P</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Together, these findings demonstrate that chrysin induces autophagy in SHED cells, suggesting its potential as a modulator of stem cell function.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102890"},"PeriodicalIF":2.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in silico anti-Alzheimer study of rutin embedded with zinc chloride loaded bovine serum albumin nanoparticles","authors":"Bibhanwita Satpathy ,&nbsp;Ivy Saha ,&nbsp;Jitu Halder ,&nbsp;Tushar Kanti Rajwar ,&nbsp;Vineet Kumar Rai ,&nbsp;Deepak Pradhan ,&nbsp;Ajit Mishra ,&nbsp;Ritu Mahanty ,&nbsp;Priyanka Dash ,&nbsp;Chandan Das ,&nbsp;Biswakanth Kar ,&nbsp;Goutam Ghosh ,&nbsp;Goutam Rath","doi":"10.1016/j.tice.2025.102869","DOIUrl":"10.1016/j.tice.2025.102869","url":null,"abstract":"<div><div>Nanotechnology-based herbal drug formulations that target multiple aspects of the disease are one of the possible approaches to address the pathological intricacies of Alzheimer’s disease (AD). The present study includes the development of rutin embedded with zinc chloride-loaded bovine serum albumin nanoparticles (R-BSA-ZnCl₂ NPs) to counter AD pathogenesis. In this work, the anti-AD potential of selected Phyto active (Rutin) was investigated using a high throughput <em>in silico</em> screening technique. Characterisation and optimization of the formulation were performed by different analytical methods such as FT-IR, zeta sizer and zeta potential, SEM analysis, thermal analysis, x-ray diffraction technique, etc. <em>In vitro</em>, free radical scavenging assay of the formulation was performed using a DPPH assay, and the ROS quantification was done by taking the RAW cell line. The amyloid β disaggregation study was confirmed by the thioflavin T assay, and the blood-brain barrier permeability assay was performed by taking brain endothelial cells. An anticholinesterase study was conducted to ascertain the formulation's potential for treating Alzheimer's disease. The NPs was prepared by ion gelation method and characterized for size (164.8 ± 5.6 nm), zeta potential (-29.6 mV), encapsulation efficiency (91 ± 1.1 %) and loading capacity (10 ± 0.2 %). The FTIR analysis indicated that there was no chemical interaction between the functional groups of rutin and other excipients. DSC, XRD studies suggested unchanged physical state of rutin in the prepared nanoparticle. Surface characterization analysed the irregular morphology of the nanoparticle. The antioxidant activity by DPPH and FRAP assay demonstrated a significant increase in free radical scavenging for R-BSA-ZnCl₂ NPs in compared to rutin (p &lt; 0.01). Anti-aggregation studies indicated that the nanoparticle inhibited the Aβ fibrils by over 70–80 %, as confirmed by Thioflavin T assay. Moreover, the blood brain barrier (BBB) permeability assay indicated permeation of nanoparticle via BBB. Thus, the present study highlighted that bovine serum albumin-zinc chloride nanoparticle may be used as a multifactorial therapeutic platform to address neurodegeneration associated with cognition.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102869"},"PeriodicalIF":2.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel cytochrome c-oxidase deficiency and mitochondrial myopathy in non-syndromic cleft lip with or without palate","authors":"Rabiatul Adawiyah Mohamad Noor ,&nbsp;Nurul Syazana Mohamad Shah ,&nbsp;Anani Aila Mat Zin ,&nbsp;Wan Azman Wan Sulaiman","doi":"10.1016/j.tice.2025.102887","DOIUrl":"10.1016/j.tice.2025.102887","url":null,"abstract":"<div><div>The role of mitochondria in regulating cells during the embryonic phase is crucial, yet their function in causing deformation to orbicularis oris muscle of cleft lip remains controversial. The study was performed to explore morphological abnormalities of orbicularis oris muscle and mitochondria in patients with cleft lip with or without palate. Seventeen cleft lip tissue samples were obtained from consented patients who underwent cleft lip repair. All tissue samples were processed according to the respective analyses: modified Gomori trichrome, cytochrome c-oxidase (COX), adenosine triphosphatase (ATP-ase), and transmission electron microscopy (TEM). Fiber types and the sizes of fibers and mitochondria were determined using ImageJ processing program. The pathological hallmark of mitochondrial myopathy, which was the presence of ragged red fibers, and the presence of novel COX-negative fibers were observed in cleft lip tissues. Muscle fiber type ratio for type II fibers was found to be predominant in orbicularis oris muscle of cleft lip tissues with 78.43%, while type I fibers was 21.57%. The diameters of the fibers were 6.37<!--> <!-->µm for type I, and 6.87<!--> <!-->µm for type II. By means of electron microscopy, minimal accumulation of mitochondria and excessive lipid droplets were noted within the myofibrils of cleft lip orbicularis oris muscle. This report on series of pathologic features of the orbicularis oris muscle and mitochondria provides insights into the possibility of impaired mitochondrial function in non-syndromic cleft lip.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102887"},"PeriodicalIF":2.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shikonin mitigates diabetic testicular dysfunction by improving oxidative, apoptotic, and metabolic functions in rats","authors":"Fatima Nawazish,&nbsp;Ali Haider,&nbsp;Imran Tarique","doi":"10.1016/j.tice.2025.102879","DOIUrl":"10.1016/j.tice.2025.102879","url":null,"abstract":"<div><div>Type 2 diabetes mellitus (T2DM) is a health concern worldwide, leading to high blood sugar levels and adversely affects various organ systems, including reproductive organs. The present study was conducted by the need to address the fact that T2DM exerts deleterious effects on male fertility via decreasing the circulating testosterone levels and increasing oxidative stress, apoptosis, and metabolic dysregulation in germ cells. The study purposes to explore the protective effect of Shikonin, a bioactive compound, on T2DM-induced reproductive damage. A high-fat diet along with streptozotocin (50 mg/kg) was administered for the induction of T2DM in male albino rats. The five experimental groups included, control, T2DM, T2DM+Shikonin (0.5 mg/kg), Only Shikonin (0.5 mg/kg), and T2DM+Metformin (50 mg/kg) were established for 4 weeks. Notably, Shikonin-treated diabetic rats exhibited significantly (p &lt; 0.0001) increased body weight, Gonadosomatic index (GSI), Testosterone, and antioxidant response reflected in the significant increase (P &lt; 0.05) in superoxide dismutase and catalase levels, while decreasing malondialdehyde. Histological analysis revealed improved testicular health in T2DM rats treated with Shikonin compared to those treated with Metformin. Shikonin treatment led to a further reduction in pro-apoptotic signaling (cytochrome c, caspase 9, and caspase 3) and improved metabolic disturbances by modulating levels of Fibroblast Growth Factor21 (FGF21) and Lactate Dehydrogenase C (LDHC) in T2DM rats. Compared with metformin, Shikonin showed the potential to offer more protective effects on male reproductive health while effectively managing diabetes, hence revealing its dual role. These findings disclose the significant (P &lt; 0.05) potential of Shikonin in protecting reproductive health against T2DM-induced oxidative stress and apoptosis, hence giving a promising avenue for the therapeutic management of diabetes-induced reproductive complications. The study emphasizes the necessity for future human trials to assess the long-term effects and dosing of Shikonin as a therapeutic option for managing T2DM and its reproductive complications.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"95 ","pages":"Article 102879"},"PeriodicalIF":2.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}