Tissue & cellPub Date : 2025-05-31DOI: 10.1016/j.tice.2025.103002
Lihua Zhang , Wan Pan , Jing Sun , Yinhong Song , Xuewei Gong
{"title":"miR-6722-3p improves sepsis-induced acute kidney injury by targeting CHI3L1","authors":"Lihua Zhang , Wan Pan , Jing Sun , Yinhong Song , Xuewei Gong","doi":"10.1016/j.tice.2025.103002","DOIUrl":"10.1016/j.tice.2025.103002","url":null,"abstract":"<div><h3>Objective</h3><div>Acute kidney injury (AKI) is a common complication of sepsis, associated with increased morbidity and mortality. Recent studies suggested microRNAs (miRNAs) play a crucial role in the pathogenesis of AKI. However, the specific role and underlying mechanisms of miR-6722-3p in AKI remain poorly understood.</div></div><div><h3>Methods</h3><div>Male Sprague Dawley rats were subjected to cecum ligation and perforation (CLP) to establish a sepsis-induced AKI model. Lentiviral vectors overexpressing miR-6722-3p or empty control vectors were administered via tail vein injection post-surgery. Hematoxylin and eosin (H&E) staining was performed to assess renal histopathological changes, while apoptosis in renal tissues was evaluated using the TUNEL assay. Serum levels of blood urea nitrogen (BUN), creatinine (Cr), and inflammatory cytokines (IL-1β and TNF-α) were measured by a biochemical instrument and ELISA, respectively. The mRNA and protein expression levels of CHI3L1, TLR4, and NF-κB p65 were analyzed using quantitative real-time PCR (qRT-PCR) and Western blotting.</div></div><div><h3>Results</h3><div>Sepsis-induced renal injury was characterized by significant histopathological damage, increased serum Cr, BUN, and inflammatory cytokines. Overexpression of miR-6722-3p suppressed inflammatory cytokine secretion, reduced renal cell apoptosis, and ameliorated renal injury. Additionally, miR-6722-3p overexpression downregulated CHI3L1, TLR4, and NF-κB p65 expression at both mRNA and protein levels.</div></div><div><h3>Conclusion</h3><div>miR-6722-3p exhibits a protective effect against sepsis-associated AKI, potentially through the modulation of CHI3L1 expression. These findings suggest that miR-6722-3p may serve as a promising therapeutic target for sepsis-induced AKI.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103002"},"PeriodicalIF":2.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tissue & cellPub Date : 2025-05-31DOI: 10.1016/j.tice.2025.102994
Abdelmonem Awad Hegazy , Eman Ramadan Abd El Fattah , Marwa Mohamed Mahmoud Abdelfattah , Marwa M. Ahmad , Dalia Ibrahim Ahmed Mesallam , Samaa Salah Abd El-Fatah
{"title":"Ameliorative effects of hesperidin against cyclophosphamide-induced ovarian dysfunction in the female rat model","authors":"Abdelmonem Awad Hegazy , Eman Ramadan Abd El Fattah , Marwa Mohamed Mahmoud Abdelfattah , Marwa M. Ahmad , Dalia Ibrahim Ahmed Mesallam , Samaa Salah Abd El-Fatah","doi":"10.1016/j.tice.2025.102994","DOIUrl":"10.1016/j.tice.2025.102994","url":null,"abstract":"<div><div>Cyclophosphamide (CP) is a commonly used antineoplastic and immunosuppressive drug that has serious adverse effects, such as ovarian damage and infertility. The aim of this study was to find out how hesperidin (HSP), a naturally occurring flavonoid with well-established health benefits, can prevent ovarian damage brought on by CP Thirtytwo adult female albino rats were divided into four groups: control, HSP-treated, CPtreated, and CP+HSP-treated. CP administration resulted in significant hormonal imbalances, including reduced level of prolactin, estrogen, FSH, and LH and decreased body and ovarian weight. Oxidative stress markers were elevated with increased ovarian malondialdehyde (MDA) and reduced antioxidant activity. Molecular analysis showed downregulation of hypothalamic (KISS-1, KISS-1r, and GnRH), hypophyseal (GnRHr) genes, and key ovarian steroidogenic enzymes. CP also upregulated ovarian P21 and NF-κB, increased immunological expression of P53 and iNOS, and caused significant histopathological and immunohistochemical changes in ovarian tissues. HSP co-administration has alleviated many of these adverse effects, improved hormonal balance, and reduced oxidative stress. This restored gene expression, and preserved ovarian structure. These results highlight the potential of HSP as a protective agent against CP-induced ovarian damage and infertility, offering promising implications for female fertility preservation during chemotherapy.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102994"},"PeriodicalIF":2.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tissue & cellPub Date : 2025-05-31DOI: 10.1016/j.tice.2025.103001
Xiuping Wang , Yiwei Weng , Yongliang Yao , Shihe Shao , Lingxiang Mao , Jianjun Wang
{"title":"Helicobacter pylori modulates macrophage polarization via the glucocorticoid receptor to promote gastric cancer cell proliferation","authors":"Xiuping Wang , Yiwei Weng , Yongliang Yao , Shihe Shao , Lingxiang Mao , Jianjun Wang","doi":"10.1016/j.tice.2025.103001","DOIUrl":"10.1016/j.tice.2025.103001","url":null,"abstract":"<div><h3>Background</h3><div><em>Helicobacter pylori</em> (<em>H. pylori</em>) is recognized as a major risk factor for gastric cancer (GC). However, the precise mechanism by which <em>H. pylori</em> influences macrophage immune responses in cancer progression remains poorly understood. This study investigates how <em>H. pylori</em> affects macrophage polarization via the glucocorticoid receptor (NR3C1) and its subsequent role in gastric cancer cell proliferation.</div></div><div><h3>Materials and methods</h3><div>NR3C1 expression, prognosis, and immune infiltration in gastric cancer were analyzed using the UCSC, TCGA, and GEPIA databases. NR3C1 expression, activation, macrophage polarization, and autophagy were assessed using RT-qPCR, Western blot, and immunofluorescence. IL-10 expression and secretion were quantified using RT-qPCR and ELISA. Reactive oxygen species (ROS) production was analyzed using flow cytometry and fluorescence microscopy. Western blot, flow cytometry, and functional assays also evaluated apoptosis, proliferation, and migration alterations.</div></div><div><h3>Results</h3><div><em>H. pylori</em> infection induces the upregulation and activation of NR3C1 in macrophages. By modulating NR3C1 signaling, <em>H. pylori</em> promotes the immunosuppressive phenotype and triggers macrophage autophagy impairment. NR3C1 presence in macrophages enhances the active response to the <em>H. pylori</em>-induced immunosuppressive microenvironment. This leads to increased activity in gastric cancer cells (HGC-27), including enhanced proliferation, migration, reduced apoptosis, and elevated ROS production.</div></div><div><h3>Conclusion</h3><div><em>H. pylori</em> orchestrates the immunosuppressive microenvironment of macrophages via NR3C1, promoting a malignant phenotype in gastric cancer cells and suggesting potential therapeutic targets for <em>H. pylori</em>-related gastric cancer treatment.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103001"},"PeriodicalIF":2.7,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tissue & cellPub Date : 2025-05-30DOI: 10.1016/j.tice.2025.102995
Farzin Abbasi , Alireza Jafarbeglou , Farshid Davoodi , Rahim Mohammadi , Omid Dezfoulian , Sayyed Jafar Hasani , Abbas Raisi , Ashkan Yahoo
{"title":"Effects of Agastache foeniculum essential oil on skin wound healing in mice","authors":"Farzin Abbasi , Alireza Jafarbeglou , Farshid Davoodi , Rahim Mohammadi , Omid Dezfoulian , Sayyed Jafar Hasani , Abbas Raisi , Ashkan Yahoo","doi":"10.1016/j.tice.2025.102995","DOIUrl":"10.1016/j.tice.2025.102995","url":null,"abstract":"<div><div>Chronic wounds are frequently hindered by high levels of reactive oxygen species, which perpetuate inflammation and delay healing. Antioxidants could mitigate oxidative stress and promote wound repair. This study investigates effectiveness of <em>Agastache foeniculum</em> essential oil in promoting skin wound healing. Thirty-six mice were divided into three groups: Sham (SH), Soybean oil (SB), and Agastache foeniculum (AGS), with each group split into excisional and incisional wounds. Treatments were applied topically for nine days. Wound contraction was measured on days 0, 3, 6, 9, 12, and 14 using ImageJ software. On day 10, biomechanical properties were assessed. Biochemical parameters, including Total Antioxidant Capacity (TAC), Total Oxidant Status (TOS), Malondialdehyde (MDA), and Glutathione Peroxidase (GPx), were evaluated on days 7 and 14. Additionally, hydroxyproline levels, Western blot analyses for TNF-α and NF-κB, and histopathological examinations were conducted. The AGS group showed a significant reduction in wound area on days 3, 9, 12, and 14. Increased strain (85.97 %), ultimate strength (9.52 N), and energy storage (13.14 J) was observed in the AGS group compared to controls. MDA levels were significantly lower (0.39, 0.36 nmol/mg protein), and TAC was significantly higher (1.68, 1.8 nmol/mg protein) in the AGS group on both days 7 and 14. Hydroxyproline levels were elevated (90.55 mg/g), and TNF-α was reduced in the AGS group, while NF-κB levels showed a non-significant decrease. Histopathological analysis indicated reduced inflammation and enhanced tissue remodeling in AGS-treated wounds. These findings suggest that <em>Agastache foeniculum</em> essential oil effectively promotes skin wound healing by enhancing contraction, strengthening biomechanical properties, reducing oxidative stress, and modulating inflammation, highlighting its potential as a therapeutic agent in wound management. Also one limitation of this study is the lack of gene expression of the markers.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102995"},"PeriodicalIF":2.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tissue & cellPub Date : 2025-05-30DOI: 10.1016/j.tice.2025.102993
Zeinab K. Aboghanima , Ashraf El Sharaby , Asmaa Aboelnour , Mohamed M.A. Abumandour , Ahmed G. Nomir
{"title":"A new anatomical insight into the architecture of biliary duct system, gall bladder, and Duodenal Papilla with the extrahepatic bile ducts of white pekin duck (Anas platyrhynchos)","authors":"Zeinab K. Aboghanima , Ashraf El Sharaby , Asmaa Aboelnour , Mohamed M.A. Abumandour , Ahmed G. Nomir","doi":"10.1016/j.tice.2025.102993","DOIUrl":"10.1016/j.tice.2025.102993","url":null,"abstract":"<div><div>The study was prepared to explain the morphological features of the biliary duct system, gall bladder, duodenal papilla, extrahepatic ducts, and pancreatic ducts of the white Pekin Duck (<em>Anas platyrhynchos</em>) using gross, casting, ultrastructural, and histochemical techniques. The gall bladder in the liver's cystic fossa is not visible from the right lobe's parietal surface. Bile is drained from the liver via the intrahepatic bile duct, with one main duct in the left lobe branched into two secondary ducts and three hepatic ducts in the right lobe. The hepatocystic duct transports bile to the gall bladder, joins the hepatic ducts, and opens in the bile sinus. The duodenal papilla is a small, non-elevated structure in the duodenal mucosal lining, containing the glands and surrounded by columnar epithelial cells and thick microvilli. The pancreatic ducts, surrounded by a thick muscular layer and opening on the elevated side of the papilla, is lined with simple epithelium and goblet cells, with microvilli. The gall bladder is composed of tunica mucosa, muscularis, serosa, and adventitia, with a simple columnar epithelium, small brush borders, and a striated border of microvilli. The hepatoenteric duct is covered by simple cuboidal epithelium, and the mucosa forms folds along its luminal surface without the mucosa lamina muscularis. The cystoenteric duct is lined with tall columnar epithelium, with a twisted wall and numerous smooth muscle fibers. PAS exhibited positive reactions for all structures. In conclusion, research on the duck biliary system is vital for understanding avian digestive mechanisms, benefiting veterinary medicine and conservation efforts.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102993"},"PeriodicalIF":2.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tissue & cellPub Date : 2025-05-29DOI: 10.1016/j.tice.2025.102999
Sawsan M.A. El-Sheikh , Mohamed Gomaa , Randa Saied , Mohamed M.M. Metwally , Heba H. Mahboub , Hanan M. Alharbi , Aaser M. Abdelazim , Hanim S. Heikal , Tarek Khamis , Azza Galal
{"title":"Benefits of fenugreek extract on renal tissue of ovariectomized rats treated with gentamicin: Biochemical, gene expression, and histopathological assessments","authors":"Sawsan M.A. El-Sheikh , Mohamed Gomaa , Randa Saied , Mohamed M.M. Metwally , Heba H. Mahboub , Hanan M. Alharbi , Aaser M. Abdelazim , Hanim S. Heikal , Tarek Khamis , Azza Galal","doi":"10.1016/j.tice.2025.102999","DOIUrl":"10.1016/j.tice.2025.102999","url":null,"abstract":"<div><div>Estrogen deprivation is linked to a diverse range of functional and histological changes in different body systems, especially kidneys. Hence, this study assesses the potential benefits of fenugreek extract (FGE) in the protection of ovariectomized rats' renal tissues, even when they received gentamicin treatment (GEN). Forty adult Wistar female rats were assigned into 5 equivalent cohorts. Group 1 (Sham): rats subjected to sham surgery; group 2 (OVX): ovariectomized rats; group 3 (OVX+ FG): ovariectomized rats administered FGE orally with dose 200 mg/kg/day; group 4 (OVX + GEN): ovariectomized rats orally received distilled water for 8 weeks, then were intraperitoneally injected with gentamycin (GEN) with dose of 40 mg /kg/day for 10 days and group 5 (OVX + FG + GEN): ovariectomized rats orally received FGE for 8 weeks and then were intraperitoneally injected with GEN for 10 days concurrently with FGE with the same previous doses. The oral administration of FGE notably decreased serum creatinine, urea, uric acid, Tumor necrosis factor (TNF-α), and Malondialdehyde (MDA), and notably elevated estrogen, Ca, Ph, and Glutathione (GSH), Total antioxidant capacity (TAC), and Interleukin 10 (IL-10) levels in the OVX+ FG and OVX + FG + GEN groups contrasted to the ovariectomized and OVX + GEN groups. Furthermore, it caused an elevation in EGF expression and a decrease in Transforming growth factor beta (TGF-β), Platelet-derived growth factor B (PDGFB), and inducible nitric oxide synthase (iNOS) expressions. The histological changes occurred by the ovariectomy and GEN treatment were improved by FGE. These findings imply that FGE, even in women receiving GEN treatment, is a useful natural treatment for the control of renal abnormalities seen in postmenopausal women.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102999"},"PeriodicalIF":2.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tissue & cellPub Date : 2025-05-29DOI: 10.1016/j.tice.2025.103000
Maria Karolina Martins Ferreira , Vladimir Galdino Sabino , José Mário Matos Sousa , Leonardo Oliveira Bittencourt , Susana Barbosa Ribeiro , Aurigena Antunes de Araújo , Carlos Augusto Galvao Barboza , Renata Duarte de Souza-Rodrigues , Marília Afonso Rabelo Buzalaf , Rafael Rodrigues Lima
{"title":"Impact of fluoride exposure at different concentrations on preosteoblastic cells: Molecular, biochemical, and morphological insights","authors":"Maria Karolina Martins Ferreira , Vladimir Galdino Sabino , José Mário Matos Sousa , Leonardo Oliveira Bittencourt , Susana Barbosa Ribeiro , Aurigena Antunes de Araújo , Carlos Augusto Galvao Barboza , Renata Duarte de Souza-Rodrigues , Marília Afonso Rabelo Buzalaf , Rafael Rodrigues Lima","doi":"10.1016/j.tice.2025.103000","DOIUrl":"10.1016/j.tice.2025.103000","url":null,"abstract":"<div><div>Fluoride can harm various tissues depending on its concentration and exposure duration. While its effects on mineralized tissues like bones and teeth are known, few studies have explored its impact on preosteoblastic cells. This study examined the effects of fluoride on differentiating osteoblastic cells. M3CT3-E1 preosteoblastic cells were cultured for 24 h or 3, 5, or 7 days with fluoride concentrations of 1, 10, or 100 μg/mL. Cell viability, oxidative stress biomarkers, mitochondrial membrane potential, cell cycle distribution, apoptosis, and morphology were assessed. After statistical analysis, At 100 μg/mL, fluoride modulated cell viability in a dose- and time-dependent manner, increasing reactive oxygen species levels and lipid peroxidation (1.382 ± 0.163 vs. 0.826 ± 0.081; p = 0.0125). Oxidative stress markers such as superoxide dismutase (SOD) (4.008 ± 0.425 vs. 0.724 ± 0.474; p = 0.0025) and glutathione (GSH) (78.38 ± 4.506 vs. 45.65 ± 2.900; p = 0.0003) were also elevated. High fluoride concentrations impaired mitochondrial activity and disrupted the cell cycle, affecting the G0/G1, S, and G2/M phases. These changes caused irreversible damage, including apoptosis and alterations in cell morphology. High fluoride concentrations can significantly damage preosteoblastic cells, reducing viability, altering redox status, impairing mitochondrial function, and disrupting the cell cycle, leading to cell death. These findings underscore that fluoride toxicity is concentration-dependent and reinforce the safety of exposure to doses of fluoride such as those found in the optimally fluoridated water.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 103000"},"PeriodicalIF":2.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144222737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tissue & cellPub Date : 2025-05-29DOI: 10.1016/j.tice.2025.102998
Xinhao Li , Xiaoyue Qiu , Li Zheng , Yuhan Liu , Jinsheng Qi
{"title":"D-β-hydroxybutyrate upregulates tight junction protein zonula occludens-1 attenuating lipopolysaccharide-stimulated cerebral microvascular hyperpermeability","authors":"Xinhao Li , Xiaoyue Qiu , Li Zheng , Yuhan Liu , Jinsheng Qi","doi":"10.1016/j.tice.2025.102998","DOIUrl":"10.1016/j.tice.2025.102998","url":null,"abstract":"<div><div>In neuroinflammation, cerebral microvascular hyperpermeability and blood-brain-barrier (BBB) damage caused by deficiency of tight junction protein Zonula Occludens-1 (ZO-1), is a common pathophysiological process in many central nervous system (CNS) diseases. The ketone body D-β-hydroxybutyrate (BHB) is known for its neuroprotective effects, but its potential role in enhancing ZO-1 generation to mitigate cerebral microvascular hyperpermeability in neuroinflammation remains unclear. Therefore, we used lipopolysaccharide (LPS)-stimulated mice and human umbilical vein endothelial cells (HUVECs) to model cerebral microvascular hyperpermeability and assess the effects of BHB on microvascular hyperpermeability and ZO-1 level. To identify mechanisms, we analyzed lysine β-hydroxybutyrylation (Kbhb) levels in cells, β-catenin binding to the <em>ZO-1</em> promoter, and co-localization of Kbhb with β-catenin. The results showed that ten days of BHB treatment upregulated ZO-1 content and reduced cerebral microvascular hyperpermeability in LPS-stimulated mice. In addition, BHB promoted ZO-1 generation and reduced paracellular permeability in LPS-stimulated HUVECs. Mechanistically, BHB (3 mmol/L) significantly increased Kbhb levels in cells, which may directly facilitate the transcription of <em>ZO-1</em>. Moreover, clear nuclear co-localization between β-catenin and Kbhb was observed, suggesting a pivotal role for β-catenin in mediating Kbhb-associated transcriptional activation of <em>ZO-1</em>. In summary, BHB may enhance histone Kbhb modification and its interaction with β-catenin, thereby promoting ZO-1 generation and mitigating LPS-induced cerebral microvascular hyperpermeability. Future development of BHB may enhance its therapeutic efficacy, thereby supporting its translational potential for the treatment of CNS diseases.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102998"},"PeriodicalIF":2.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tissue & cellPub Date : 2025-05-28DOI: 10.1016/j.tice.2025.102997
Mohammed I.A. Ibrahim , Rephima M. Phaswane , Antoinette V. Lensink , Christo J. Botha
{"title":"Impact of pubertal imidacloprid exposure on the genital tract of Japanese quail (Coturnix Coturnix japonica): Histomorphometric and ultrastructural study","authors":"Mohammed I.A. Ibrahim , Rephima M. Phaswane , Antoinette V. Lensink , Christo J. Botha","doi":"10.1016/j.tice.2025.102997","DOIUrl":"10.1016/j.tice.2025.102997","url":null,"abstract":"<div><h3>Background</h3><div>The effects of exposure to the neonicotinoid pesticide, imidacloprid (IMI), on the histopathology and ultrastructure of the genital tracts of pubertal male and female Japanese quails (<em>Coturnix Coturnix japonica</em>) were investigated.</div></div><div><h3>Methods</h3><div>Pubertal quail (<em>n = 28</em>), commencing at 5 weeks post-hatching, were orally gavaged with 1.55 (low), 3.1 (medium), and 6.2 (high) mg/kg IMI, dissolved in distilled water, twice a week for four weeks. Control birds received distilled water only. Samples collected from male and female genital tracts included epididymal duct and magnum and shell gland. Histopathology, histometric measurements, as well as transmission and scanning electron microscopy were performed.</div></div><div><h3>Results and conclusion</h3><div>The medium and high IMI doses induced pathomorphological changes in the epithelial cells of the magnum and shell gland, which included a ciliary loss, presence of intracytoplasmic vacuoles, mitochondrial damage, and fewer secretory granules. In addition, there were cytoplasmic vacuoles detected in the efferent ducts and epididymal ducts of quails treated with medium and high IMI doses. The epithelial heights of the magnum, shell gland, rete testis and proximal efferent duct were reduced (<em>P ≤ 0.05</em>) in all IMI treatment groups. In addition, the epididymal duct epithelial heights decreased (<em>P ≤ 0.05</em>) at medium and high IMI doses, but changes in the distal efferent ducts were only observed in the high dose group. Overall, the results confirmed that pubertal exposure to IMI induced dose-dependent pathomorphological changes in male and female quail genital tracts, suggesting that IMI might impact their fertility and reproductive function.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102997"},"PeriodicalIF":2.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tissue & cellPub Date : 2025-05-28DOI: 10.1016/j.tice.2025.102996
Nashwa Barakat , Karima Elsharkawy , Aml Awad , Salma M. Khirallah
{"title":"Daxas provides renoprotective effects in ischemia-reperfusion injury by targeting apoptosis, oxidative stress, and inflammation pathways","authors":"Nashwa Barakat , Karima Elsharkawy , Aml Awad , Salma M. Khirallah","doi":"10.1016/j.tice.2025.102996","DOIUrl":"10.1016/j.tice.2025.102996","url":null,"abstract":"<div><h3>Background and aim</h3><div>The pathophysiology of renal ischemia-reperfusion injury (IRI) is a multifaceted process involving various pathways, including oxidative stress and inflammatory responses. This study was designed to evaluate the renoprotective effect of Daxas, Roflumilast (RFL), a selective phosphodiesterase 4 (PDE4) inhibitor, in preventing IRI consequences in rats.</div></div><div><h3>Methods</h3><div>Fifty-four rats were split up into three groups: the sham group, which did not experience any ischemia; the IRI group, which underwent renal IRI for 45 minutes; and the Daxas-treated group, which received 1.2 mg/kg after IRI. At 2, 5, and 7 days, blood and kidney samples were collected to assess renal histopathology, oxidative stress indicators, apoptosis, inflammatory gene analysis, and kidney function.</div></div><div><h3>Results</h3><div>The outcomes showed that renal IRI significantly impaired kidney function. Furthermore, in a comparison with the sham group, IRI significantly raised the levels of oxidative stress marker malonaldehyde (MDA) in the kidney while significantly decreasing the activities of glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) at 2, 5, and 7 days (p < 0.05). In comparison to the Sham group, IRI also caused a drop in Bcl2 and Nrf2 and an increase in BAX, MPO, IL-6, and TNF-α at the different time intervals (p < 0.05). At different time points, treatment with Daxas significantly improved all of these metrics when compared to the IRI group (p < 0.05). Immunohistochemical examination of BAX demonstrated a substantial elevation in the IRI group relative to the sham group (p < 0.05), concentrated in tubular epithelial cells and inflammatory infiltrates. Daxas therapy markedly decreased BAX expression to levels akin to the sham group (p < 0.05).</div></div><div><h3>Conclusions</h3><div>Daxas demonstrated potent kidney-protective benefits by reducing oxidative stress, enhancing renal function, and modifying inflammatory and apoptotic pathways. According to these results, Daxas may be a viable treatment choice for reducing the consequences of IRI.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"96 ","pages":"Article 102996"},"PeriodicalIF":2.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}