Benefits of fenugreek extract on renal tissue of ovariectomized rats treated with gentamicin: Biochemical, gene expression, and histopathological assessments

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-05-29 DOI:10.1016/j.tice.2025.102999

Sawsan M.A. El-Sheikh , Mohamed Gomaa , Randa Saied , Mohamed M.M. Metwally , Heba H. Mahboub , Hanan M. Alharbi , Aaser M. Abdelazim , Hanim S. Heikal , Tarek Khamis , Azza Galal

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引用次数: 0

Abstract

Estrogen deprivation is linked to a diverse range of functional and histological changes in different body systems, especially kidneys. Hence, this study assesses the potential benefits of fenugreek extract (FGE) in the protection of ovariectomized rats' renal tissues, even when they received gentamicin treatment (GEN). Forty adult Wistar female rats were assigned into 5 equivalent cohorts. Group 1 (Sham): rats subjected to sham surgery; group 2 (OVX): ovariectomized rats; group 3 (OVX+ FG): ovariectomized rats administered FGE orally with dose 200 mg/kg/day; group 4 (OVX + GEN): ovariectomized rats orally received distilled water for 8 weeks, then were intraperitoneally injected with gentamycin (GEN) with dose of 40 mg /kg/day for 10 days and group 5 (OVX + FG + GEN): ovariectomized rats orally received FGE for 8 weeks and then were intraperitoneally injected with GEN for 10 days concurrently with FGE with the same previous doses. The oral administration of FGE notably decreased serum creatinine, urea, uric acid, Tumor necrosis factor (TNF-α), and Malondialdehyde (MDA), and notably elevated estrogen, Ca, Ph, and Glutathione (GSH), Total antioxidant capacity (TAC), and Interleukin 10 (IL-10) levels in the OVX+ FG and OVX + FG + GEN groups contrasted to the ovariectomized and OVX + GEN groups. Furthermore, it caused an elevation in EGF expression and a decrease in Transforming growth factor beta (TGF-β), Platelet-derived growth factor B (PDGFB), and inducible nitric oxide synthase (iNOS) expressions. The histological changes occurred by the ovariectomy and GEN treatment were improved by FGE. These findings imply that FGE, even in women receiving GEN treatment, is a useful natural treatment for the control of renal abnormalities seen in postmenopausal women.

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胡芦巴提取物对庆大霉素治疗去卵巢大鼠肾组织的益处：生化、基因表达和组织病理学评估

雌激素缺乏与不同身体系统的多种功能和组织学变化有关，尤其是肾脏。因此，本研究评估胡芦巴提取物（FGE）在保护去卵巢大鼠肾组织方面的潜在益处，即使它们接受庆大霉素治疗（GEN）。40只成年Wistar雌性大鼠被分成5组。1组（Sham）：假手术大鼠；2组（OVX）：去卵巢大鼠；第三组（OVX+ FG）：去卵巢大鼠口服FGE，剂量200 mg/kg/day；第4组（OVX + GEN）：去卵巢大鼠口服蒸馏水8周，然后腹腔注射庆大霉素（GEN），剂量为40 mg /kg/天，连续10天；第5组（OVX + FG + GEN）：去卵巢大鼠口服FGE 8周，然后在FGE的同时腹腔注射GEN，剂量相同，连续10天。与去卵巢组和去卵巢组相比，口服FGE显著降低血清肌酐、尿素、尿酸、肿瘤坏死因子（TNF-α）和丙二醛（MDA），显著升高雌激素、钙、Ph和谷胱甘肽（GSH）、总抗氧化能力（TAC）和白细胞介素10 （IL-10）水平。此外，它还导致EGF表达升高，转化生长因子β (TGF-β)、血小板衍生生长因子B （PDGFB）和诱导型一氧化氮合酶（iNOS）表达降低。FGE改善了卵巢切除术和GEN治疗后的组织学改变。这些发现表明，FGE，即使在接受GEN治疗的妇女中，也是一种有效的自然疗法，可用于控制绝经后妇女的肾脏异常。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.