聚甘油癸二酸/聚乳酸(PGS/PLA)水凝胶联合高压氧治疗可促进糖尿病大鼠全层创面愈合

IF 2.5 4区 生物学 Q1 ANATOMY & MORPHOLOGY
Waheeb S. Aggad , Amany I. Almars , Fayez Alsulaimani , Ahmed M. Basri , Sameerah Shaheen , Nahlah M. Ghouth , Nada M. Ghouth , Zaki H. Hakami , Wajnat A. Tounsi , Sawsan Abd Ellatif , Mona H. Soliman , Hailah M. Almohaimeed
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引用次数: 0

摘要

慢性伤口,特别是糖尿病患者,由于缺血条件下的愈合受损,给治疗带来了重大挑战。本研究探讨了聚癸二酸甘油/聚乳酸(PGS/PLA)水凝胶联合高压氧治疗(HBOT)对糖尿病大鼠模型创面愈合的协同作用。采用PGS/PLA、HBOT或两者联合治疗大鼠背部全层皮肤损伤。使用体视技术进行组织学评估,量化成纤维细胞、中性粒细胞和血管。马松三色染色法测定胶原蛋白含量。分析创面抗张强度、氧化应激生物标志物(GSH、SOD、CAT、MDA)及相关基因(TGF-β、VEGF、bFGF、HIF-1α、TNF-α、IL-1β)表达水平。结果显示,与其他组相比,PGS/PLA+HBOT组显著改善了创面闭合,增加了成纤维细胞密度,减少了中性粒细胞计数(p <; 0.05)。联合治疗组血管密度和胶原沉积最高(p <; 0.05)。生物力学分析显示,所有治疗组的抗拉强度均有所提高,其中联合治疗组优于其他治疗组(p <; 0.05)。抗氧化剂水平显著升高,MDA水平降低,尤其是PGS/PLA+HBOT组(p <; 0.05)。基因表达分析显示,治疗后创面TGF-β、VEGF、bFGF水平升高,HIF-1α、TNF-α、IL-1β表达降低(p <; 0.05)。PGS/PLA纳米纤维敷料与HBOT结合,通过促进细胞增殖、血管生成、胶原形成、氧化还原平衡以及调节炎症和再生基因表达,有效加速伤口愈合。这种策略可能为治疗缺血性伤口提供一种有希望的治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Poly glycerol sebacate/poly lactide acid (PGS/PLA) hydrogel in combination with hyperbaric oxygen therapy improved full thickness wound healing in diabetic rat
Chronic wounds, particularly in diabetic patients, pose significant therapeutic challenges due to impaired healing under ischemic conditions. This study investigated the synergistic effect of a poly glycerol sebacate/poly lactide acid (PGS/PLA) hydrogel combined with hyperbaric oxygen therapy (HBOT) on wound healing in a diabetic rat model. Full-thickness skin wounds were induced on the dorsum of rats and treated with PGS/PLA, HBOT, or their combination. Histological evaluations were performed using stereological techniques to quantify fibroblasts, neutrophils, and blood vessels. Collagen content was assessed by Masson's trichrome staining. Wound tensile strength, oxidative stress biomarkers (GSH, SOD, CAT, MDA), and expression levels of related genes (TGF-β, VEGF, bFGF, HIF-1α, TNF-α, IL-1β) were also analyzed. The results showed that the PGS/PLA+HBOT group significantly improved wound closure, increased fibroblast density, and reduced neutrophil count compared to other groups (p < 0.05). Vascular density and collagen deposition were highest in the combined treatment group (p < 0.05). Biomechanical analysis revealed enhanced tensile strength in all treated groups, with the combination group outperforming the others (p < 0.05). Antioxidant levels were significantly elevated, while MDA levels were decreased, particularly in the PGS/PLA+HBOT group (p < 0.05). Gene expression analysis showed increased levels of TGF-β, VEGF, and bFGF, and reduced expression of HIF-1α, TNF-α, and IL-1β in treated wounds (p < 0.05). The combination of PGS/PLA nanofiber dressing with HBOT effectively accelerates wound healing by enhancing cellular proliferation, angiogenesis, collagen formation, redox balance, and modulation of inflammatory and regenerative gene expression. This strategy may offer a promising therapeutic approach for managing ischemic wounds.
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来源期刊
Tissue & cell
Tissue & cell 医学-解剖学与形态学
CiteScore
3.90
自引率
0.00%
发文量
234
期刊介绍: Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.
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