Waheeb S. Aggad , Amany I. Almars , Fayez Alsulaimani , Ahmed M. Basri , Sameerah Shaheen , Nahlah M. Ghouth , Nada M. Ghouth , Zaki H. Hakami , Wajnat A. Tounsi , Sawsan Abd Ellatif , Mona H. Soliman , Hailah M. Almohaimeed
{"title":"聚甘油癸二酸/聚乳酸(PGS/PLA)水凝胶联合高压氧治疗可促进糖尿病大鼠全层创面愈合","authors":"Waheeb S. Aggad , Amany I. Almars , Fayez Alsulaimani , Ahmed M. Basri , Sameerah Shaheen , Nahlah M. Ghouth , Nada M. Ghouth , Zaki H. Hakami , Wajnat A. Tounsi , Sawsan Abd Ellatif , Mona H. Soliman , Hailah M. Almohaimeed","doi":"10.1016/j.tice.2025.103113","DOIUrl":null,"url":null,"abstract":"<div><div>Chronic wounds, particularly in diabetic patients, pose significant therapeutic challenges due to impaired healing under ischemic conditions. This study investigated the synergistic effect of a poly glycerol sebacate/poly lactide acid (PGS/PLA) hydrogel combined with hyperbaric oxygen therapy (HBOT) on wound healing in a diabetic rat model. Full-thickness skin wounds were induced on the dorsum of rats and treated with PGS/PLA, HBOT, or their combination. Histological evaluations were performed using stereological techniques to quantify fibroblasts, neutrophils, and blood vessels. Collagen content was assessed by Masson's trichrome staining. Wound tensile strength, oxidative stress biomarkers (GSH, SOD, CAT, MDA), and expression levels of related genes (TGF-β, VEGF, bFGF, HIF-1α, TNF-α, IL-1β) were also analyzed. The results showed that the PGS/PLA+HBOT group significantly improved wound closure, increased fibroblast density, and reduced neutrophil count compared to other groups (p < 0.05). Vascular density and collagen deposition were highest in the combined treatment group (p < 0.05). Biomechanical analysis revealed enhanced tensile strength in all treated groups, with the combination group outperforming the others (p < 0.05). Antioxidant levels were significantly elevated, while MDA levels were decreased, particularly in the PGS/PLA+HBOT group (p < 0.05). Gene expression analysis showed increased levels of TGF-β, VEGF, and bFGF, and reduced expression of HIF-1α, TNF-α, and IL-1β in treated wounds (p < 0.05). The combination of PGS/PLA nanofiber dressing with HBOT effectively accelerates wound healing by enhancing cellular proliferation, angiogenesis, collagen formation, redox balance, and modulation of inflammatory and regenerative gene expression. This strategy may offer a promising therapeutic approach for managing ischemic wounds.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"98 ","pages":"Article 103113"},"PeriodicalIF":2.5000,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Poly glycerol sebacate/poly lactide acid (PGS/PLA) hydrogel in combination with hyperbaric oxygen therapy improved full thickness wound healing in diabetic rat\",\"authors\":\"Waheeb S. Aggad , Amany I. Almars , Fayez Alsulaimani , Ahmed M. Basri , Sameerah Shaheen , Nahlah M. Ghouth , Nada M. Ghouth , Zaki H. Hakami , Wajnat A. Tounsi , Sawsan Abd Ellatif , Mona H. Soliman , Hailah M. Almohaimeed\",\"doi\":\"10.1016/j.tice.2025.103113\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Chronic wounds, particularly in diabetic patients, pose significant therapeutic challenges due to impaired healing under ischemic conditions. This study investigated the synergistic effect of a poly glycerol sebacate/poly lactide acid (PGS/PLA) hydrogel combined with hyperbaric oxygen therapy (HBOT) on wound healing in a diabetic rat model. Full-thickness skin wounds were induced on the dorsum of rats and treated with PGS/PLA, HBOT, or their combination. Histological evaluations were performed using stereological techniques to quantify fibroblasts, neutrophils, and blood vessels. Collagen content was assessed by Masson's trichrome staining. Wound tensile strength, oxidative stress biomarkers (GSH, SOD, CAT, MDA), and expression levels of related genes (TGF-β, VEGF, bFGF, HIF-1α, TNF-α, IL-1β) were also analyzed. The results showed that the PGS/PLA+HBOT group significantly improved wound closure, increased fibroblast density, and reduced neutrophil count compared to other groups (p < 0.05). Vascular density and collagen deposition were highest in the combined treatment group (p < 0.05). Biomechanical analysis revealed enhanced tensile strength in all treated groups, with the combination group outperforming the others (p < 0.05). Antioxidant levels were significantly elevated, while MDA levels were decreased, particularly in the PGS/PLA+HBOT group (p < 0.05). Gene expression analysis showed increased levels of TGF-β, VEGF, and bFGF, and reduced expression of HIF-1α, TNF-α, and IL-1β in treated wounds (p < 0.05). The combination of PGS/PLA nanofiber dressing with HBOT effectively accelerates wound healing by enhancing cellular proliferation, angiogenesis, collagen formation, redox balance, and modulation of inflammatory and regenerative gene expression. This strategy may offer a promising therapeutic approach for managing ischemic wounds.</div></div>\",\"PeriodicalId\":23201,\"journal\":{\"name\":\"Tissue & cell\",\"volume\":\"98 \",\"pages\":\"Article 103113\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-08-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tissue & cell\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0040816625003957\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue & cell","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0040816625003957","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
Poly glycerol sebacate/poly lactide acid (PGS/PLA) hydrogel in combination with hyperbaric oxygen therapy improved full thickness wound healing in diabetic rat
Chronic wounds, particularly in diabetic patients, pose significant therapeutic challenges due to impaired healing under ischemic conditions. This study investigated the synergistic effect of a poly glycerol sebacate/poly lactide acid (PGS/PLA) hydrogel combined with hyperbaric oxygen therapy (HBOT) on wound healing in a diabetic rat model. Full-thickness skin wounds were induced on the dorsum of rats and treated with PGS/PLA, HBOT, or their combination. Histological evaluations were performed using stereological techniques to quantify fibroblasts, neutrophils, and blood vessels. Collagen content was assessed by Masson's trichrome staining. Wound tensile strength, oxidative stress biomarkers (GSH, SOD, CAT, MDA), and expression levels of related genes (TGF-β, VEGF, bFGF, HIF-1α, TNF-α, IL-1β) were also analyzed. The results showed that the PGS/PLA+HBOT group significantly improved wound closure, increased fibroblast density, and reduced neutrophil count compared to other groups (p < 0.05). Vascular density and collagen deposition were highest in the combined treatment group (p < 0.05). Biomechanical analysis revealed enhanced tensile strength in all treated groups, with the combination group outperforming the others (p < 0.05). Antioxidant levels were significantly elevated, while MDA levels were decreased, particularly in the PGS/PLA+HBOT group (p < 0.05). Gene expression analysis showed increased levels of TGF-β, VEGF, and bFGF, and reduced expression of HIF-1α, TNF-α, and IL-1β in treated wounds (p < 0.05). The combination of PGS/PLA nanofiber dressing with HBOT effectively accelerates wound healing by enhancing cellular proliferation, angiogenesis, collagen formation, redox balance, and modulation of inflammatory and regenerative gene expression. This strategy may offer a promising therapeutic approach for managing ischemic wounds.
期刊介绍:
Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed.
Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.