蜂胶水提物在成骨细胞前分化过程中调节ECM重塑和钙水平

IF 2.5 4区 生物学 Q1 ANATOMY & MORPHOLOGY
Paula Bertin de Morais , Gerson Santos de Almeida , Ricardo de Oliveira Orsi , Willian Fernando Zambuzzi , Célio Jr. Da Costa Fernandes
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引用次数: 0

摘要

骨质疏松症和其他骨质流失相关疾病仍然是主要的健康问题,强调了对创新再生疗法的需求。蜂胶是一种富含咖啡酸苯乙酯(CAPE)等生物活性化合物的天然树脂物质,已成为调节骨再生的有希望的候选物质。然而,其影响成骨细胞功能的细胞和分子机制尚未完全阐明。在这项研究中,我们评估了蜂胶水提取物对成骨前细胞粘附、增殖、分化、细胞外基质(ECM)重塑和炎症的影响。我们的研究结果表明,虽然蜂胶处理没有改变细胞粘附或细胞骨架组织,但它通过上调CDK2表达和磷酸化显著增强细胞增殖,提示刺激G1/S细胞周期进程。分化分析表明,成骨标志物的表达增加,包括BMP7、Runx2和Osterix,以及RankL/OPG轴的调节。尽管矿化程度有限,但我们观察到胶原沉积增强,ECM相关蛋白(如骨唾液蛋白(BSP))上调,同时MMP9活性增加,表明ECM重构活跃。此外,蜂胶诱导抗炎细胞因子谱,其特征是IL-10升高,促炎细胞因子IL-6、IL-18和TNF-α水平降低。总之,我们的研究结果表明蜂胶水溶液提取物通过调节细胞周期进程、增强成骨细胞分化、重塑ECM和减少炎症来促进成骨表型。这些结果支持蜂胶作为一种天然治疗剂在骨组织工程和再生医学中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Aqueous extracts of propolis modulate ECM remodeling and calcium levels during pre-osteoblast differentiation
Osteoporosis and other bone-loss-related disorders remain major health concerns, underscoring the demand for innovative regenerative therapies. Propolis, a natural resinous substance rich in bioactive compounds such as caffeic acid phenethyl ester (CAPE), has emerged as a promising candidate for modulating bone regeneration. However, the cellular and molecular mechanisms underlying its effects on osteoblast function are not fully elucidated. In this study, we evaluated the impact of an aqueous extract of propolis on pre-osteoblast adhesion, proliferation, differentiation, extracellular matrix (ECM) remodeling, and inflammation. Our results showed that while propolis treatment did not alter cell adhesion or cytoskeletal organization, it significantly enhanced cell proliferation through the upregulation of CDK2 expression and phosphorylation, suggesting stimulation of G1/S cell cycle progression. Differentiation analyses demonstrated increased expression of osteogenic markers, including BMP7, Runx2, and Osterix, as well as modulation of the RankL/OPG axis. Despite limited mineralization, we observed enhanced collagen deposition and upregulation of ECM-related proteins, such as bone sialoprotein (BSP), alongside increased MMP9 activity, indicating active ECM remodeling. Moreover, propolis induced an anti-inflammatory cytokine profile, characterized by elevated IL-10 and reduced levels of pro-inflammatory cytokines IL-6, IL-18, and TNF-α. Altogether, our findings suggest that aqueous propolis extract promotes an osteogenic phenotype by modulating cell cycle progression, enhancing osteoblastic differentiation, remodeling the ECM, and reducing inflammation. These results support the potential use of propolis as a natural therapeutic agent in bone tissue engineering and regenerative medicine.
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来源期刊
Tissue & cell
Tissue & cell 医学-解剖学与形态学
CiteScore
3.90
自引率
0.00%
发文量
234
期刊介绍: Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.
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