Systematic and applied microbiology最新文献

{"title":"Genome-based taxonomy of the family Haloarculaceae, proposal of Natronomonadaceae fam. nov., and description of four novel halophilic archaea from two saline lakes and a marine solar saltern","authors":"Ling-Rui Zhu,&nbsp;Ya-Ling Mao,&nbsp;Yao Hu,&nbsp;Ya-Ping Sun,&nbsp;Jing Hou,&nbsp;Heng-Lin Cui","doi":"10.1016/j.syapm.2025.126592","DOIUrl":"10.1016/j.syapm.2025.126592","url":null,"abstract":"<div><div>A new family related to the family <em>Haloarculaceae</em> was proposed and the genus <em>Actinarchaeum</em> was merged into the genus <em>Halocatena</em> through phylogenetic, phylogenomic, and comparative genomic analyses. Four strains KK48^T, YCN56^T, SYNS191^T, and SYNS196^T with new taxonomic status were isolated from inland saline lakes and a marine solar saltern. According to the comparison of 16S rRNA gene and <em>rpoB'</em> gene sequences, strains KK48^T, YCN56^T, SYNS191^T, and SYNS196^T showed high sequence similarities to the genera <em>Salinibaculum</em> and <em>Salinirubellus</em>, respectively. The values of average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity between these strains and the species of <em>Salinibaculum</em> and <em>Salinirubellus</em> ranged from 75.3 to 77.7 %, 24.5–25.9 % and 66.3–73.4 %, respectively. These data were well below the threshold for species classification, supporting their placements in new taxa. The major polar lipids of these strains were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glucosyl diether, mannosyl glucosyl diether, and disulfated mannosyl glucosyl diether. Based on the phenotypic, chemotaxonomic, phylogenetic, and phylogenomic properties, strains KK48^T (= CGMCC 1.19060^T = JCM 35607^T), YCN56^T (= CGMCC 1.62603^T = JCM 36493^T), SYNS191^T (= CGMCC 1.62607^T = JCM 36494^T), and SYNS196^T (= CGMCC 1.62608^T = JCM 36495^T) represent four novel species of the genera <em>Salinibaculum</em> and <em>Salinirubellus</em>. And <em>Salinibaculum rarum</em> sp. nov., <em>Salinibaculum salinum</em> sp. nov., <em>Salinibaculum marinum</em> sp. nov., and <em>Salinirubellus litoreus</em> sp. nov. are proposed to accommodate these strains.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126592"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomics of Elusimicrobiaceae (phylum Elusimicrobiota) and description of the isolates Elusimicrobium simillimum sp. nov., Elusimicrobium posterum sp. nov., and Parelusimicrobium proximum gen. nov. sp. nov.","authors":"Undine S. Mies ,&nbsp;Hao Zheng ,&nbsp;Katja Platt ,&nbsp;Renate Radek ,&nbsp;Nicole Paczia ,&nbsp;Sebastian C. Treitli ,&nbsp;Andreas Brune","doi":"10.1016/j.syapm.2025.126606","DOIUrl":"10.1016/j.syapm.2025.126606","url":null,"abstract":"<div><div>The tree of life comprises many deep-branching lineages with no or only very few cultured representatives. One such lineage is the phylum <em>Elusimicrobiota</em>, which contains only two described species and whose biology has been only poorly explored. We isolated three new species from this phylum from the intestinal tracts of cockroaches. Like their closest relative, <em>Elusimicrobium minutum</em>, the only member of the family <em>Elusimicrobiaceae</em> described to date, they are small, pleomorphic gram-negative rods characterized by a distinct cell cycle, and like all ultramicrobacteria, they pass through a 0.22-μm filter membrane. Physiological characterization of all isolates revealed that they are obligately anaerobic fermenters that lack catalase and cytochrome <em>c</em> oxidase activities but can remove oxygen from their environment in a non-respiratory manner. Their substrate range is limited to a few hexoses, such as <span>d</span>-glucose, <span>d</span>-galactose, and <em>N</em>-acetyl-<span>d</span>-glucosamine, which are fermented to lactate, acetate, ethanol, and hydrogen as major products. Comparative genome analysis, which included more than 100 MAGs of uncultured lineages of <em>Elusimicrobiaceae</em>, revealed the underlying metabolic pathways and outlined a new phylogenomic framework of the family. Based on phylogenomic, physiological, and morphological evidence, we describe the new isolates as <em>Parelusimicrobium proximum</em> gen. nov., sp. nov., <em>Elusimicrobium posterum</em> sp. nov., and <em>Elusimicrobium simillimum</em> sp. nov. under the rules of ICNP. Based on high-quality genomes of all uncultured representatives, we propose a comprehensive taxonomy of all lineages in the family under the rules of SeqCode, including the new genera <em>Avelusimicrobium</em>, <em>Proelusimicrobium</em>, and the candidate genus “<em>Pseudelusimicrobium</em>”.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126606"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “Characterization of two novel species of the genusBifidobacterium: Bifidobacterium saimiriisciurei sp. nov. and Bifidobacterium platyrrhinorum sp. nov.” [Syst. Appl. Microbiol. 43 (2020) 126111]","authors":"Monica Modesto ,&nbsp;Maria Satti ,&nbsp;Koichi Watanabe ,&nbsp;Donatella Scarafile ,&nbsp;Chien-Hsun Huang ,&nbsp;Jong-Shian Liou ,&nbsp;Tomohiko Tamura ,&nbsp;Satomi Saito ,&nbsp;Mizuki Watanabe ,&nbsp;Koji Mori ,&nbsp;Lina Huang ,&nbsp;Camillo Sandri ,&nbsp;Caterina Spiezio ,&nbsp;Masanori Arita ,&nbsp;Paola Mattarelli","doi":"10.1016/j.syapm.2025.126601","DOIUrl":"10.1016/j.syapm.2025.126601","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126601"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel thermophilic hydrolytic bacterium Rarispira pelagica gen. nov., sp. nov., reclassification of Spirochaeta thermophila as Winmispira thermophila gen. nov., comb. nov., and proposal of Winmispiraceae fam. nov. and Winmispirales ord. nov. in the class Spirochaetia","authors":"Olga A. Podosokorskaya ,&nbsp;Anastasiia I. Maltseva ,&nbsp;Alexander G. Elcheninov ,&nbsp;Andrei A. Novikov ,&nbsp;Kseniya S. Zayulina ,&nbsp;Alexander Y. Merkel","doi":"10.1016/j.syapm.2025.126604","DOIUrl":"10.1016/j.syapm.2025.126604","url":null,"abstract":"<div><div>A novel anaerobic moderately thermophilic bacterium, strain 38H-sp^T, was isolated from a hot spring located 12 m deep of the Kunashir Island shore. Gram-stain negative cells were non-sporeforming, motile, thin helices with regular coiling, occasionally forming bundles of cells. The strain grew at 30–60 °C and pH range of 5.5–8.4 with an optimum at 55 °C and pH 6.6–7.0. Strain 38H-sp^T required 0.5–5.5% NaCl (1.5% is an optimum) for growth. It was a chemoorganoheterotroph, growing on carbohydrates, including polymers (starch, pullulan, xylan, cellulose, arabinoxylan, xanthan gum, mannan, galactomannan, alginate and laminarin). Major products of glucose fermentation were acetate, ethanol, hydrogen, and carbon dioxide. Major cellular fatty acids were iso-C_15:0, C_14:0, C_12:1 DMA. The size of complete genome of strain 38H-sp^T was 2.35 Mb; DNA G + C content was 40.1%. 93 CAZymes including 54 glycoside hydrolases were found to be encoded in the genome of the strain. According to 16S rRNA gene sequence and conserved protein sequences phylogenies strain 38H-sp^T with its closest relative <em>Spirochaeta thermophila</em> represented a deeply branched lineage of the class <em>Spirochaetia.</em> Based on phylogenetic analysis and phenotypic features these two bacteria were assigned to a novel family within a novel order for that the names <em>Winmispiraceae</em> fam. nov. and <em>Winmispirales</em> ord. nov. are proposed with <em>Winmispira thermophila</em> gen. nov., comb. nov. (previously known as <em>Spirochaeta thermophila</em>) as type species. Strain 38H-sp^T (=DSM 100344^T = VKM B-2965^T) represents the novel genus and species <em>Rarispira pelagica</em> gen. nov., sp. nov.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126604"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143682041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natronomicrosphaera hydrolytica, gen. nov., sp. nov., a first representative of the phylum Planctomycetota from soda lakes","authors":"Dimitry Y. Sorokin ,&nbsp;Alexander Y. Merkel ,&nbsp;Nicole J. Bale ,&nbsp;Michel Koenen ,&nbsp;Jaap S. Sinninghe Damsté ,&nbsp;Laura Marturano ,&nbsp;Enzo Messina ,&nbsp;Violetta La Cono ,&nbsp;Michail M. Yakimov","doi":"10.1016/j.syapm.2025.126608","DOIUrl":"10.1016/j.syapm.2025.126608","url":null,"abstract":"<div><div>Despite intensive microbiological characterization of soda lake microbial communities, no culturable representatives from the phylum <em>Planctomycetota</em> have been isolated from these haloalkaline habitats. In the context of studying polysaccharide utilization by soda lake microbial communities, we used polysaccharide hyaluronic acid as enrichment substrate at aerobic, moderate haloalkaline conditions (1 M total Na⁺, pH 9.5). This resulted in a selective enrichment and isolation in pure culture of a bacterial strain AB-hyl4 belonging to <em>Planctomycetota</em>. The cells are tiny motile cocci growing in large aggregates, with the Gram-negative type of ultrastructure and producing a yellow pigment. This obligate aerobic saccharolytic heterotroph has an extremely narrow growth substrate range including, besides hyaluronic acid, melezitose and glycerol. The membrane lipids consist of phosphatidylcholine and two types of neutral lipids, including hopanoids and monounsaturated C17 and C19 hydrocarbons. Phylogenomic analysis placed the isolate into the family <em>Phycisphaeraceae</em>, class <em>Phycisphaerae,</em> as a new genus-level lineage. Its genome contained a gene encoding a polysaccharide lyase from the PL8 family which is probably responsible for the degradation of hyaluronic acid to a dimer, followed by its transport and hydrolysis into monomers in periplasm and final glycolytic degradation in cytoplasm. On the basis of distinct phenotypic and genomic properties, strain AB-hyl4^T (DSM 117794 = UQM 41914) is proposed to be classified as <em>Natronomicrosphaera hydrolytica</em> gen. nov., sp. nov.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126608"},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tenuifilum osseticum sp. nov., a novel thermophilic hydrolytic bacterium within the Tenuifilaceae isolated from a North Ossetian thermal spring, and emended description of the genus Tenuifilum","authors":"Olga A. Podosokorskaya ,&nbsp;Maria I. Prokofeva ,&nbsp;Alexandra A. Kuchierskaya ,&nbsp;Alexandra A. Klyukina ,&nbsp;Alexander G. Elcheninov","doi":"10.1016/j.syapm.2025.126591","DOIUrl":"10.1016/j.syapm.2025.126591","url":null,"abstract":"<div><div>A novel anaerobic moderately thermophilic bacterium, strain 4138-str^T, was isolated from a thermal spring of North Ossetia (Russian Federation). Gram-negative cells were non-sporeforming, straight or curved filamentous rods, occasionally forming rosettes. The strain grew at 30–55 °C, pH range of 6.1–8.7, NaCl range of 0–4 %, with an optimum at 50 °C, pH 7.1–7.5 and 0.2–0.4 % NaCl. It was a chemoorganoheterotroph, growing on simple sugars (glucose, maltose, cellobiose, etc.) and carbohydrates (starch, pullulan, laminarin, xylan, lichenan, curdlan, pachyman) or proteinaceous substrates (peptone, tryptone, gelatin, casein). Sulfur was used as electron acceptor. Major products of glucose fermentation were acetate, hydrogen, and carbon dioxide. Major cellular fatty acids were iso-C_15:0 and anteiso-C_15:0. The quinone was MK-7. The size of the whole genome of strain 4138-str^T was 3.275 Mbp; DNA G + C content was 42.1 %. Genome analysis allowed to identify genes encoding carbohydrate-active enzymes and extracellular proteases. In addition, central metabolism and fermentation pathways of strain 4138-str^T were reconstructed. According to both phylogenetic analyses, based on 16S rRNA gene sequences and conserved protein sequences, as well as genome-based comparisons, strain 4138-str^T formed a species-level lineage within <em>Tenuifilum</em> genus of <em>Tenuifilaceae</em> family (phylum <em>Bacteroidota</em>). Here we propose a novel species <em>Tenuifilum osseticum</em> sp. nov. with type strain 4138-str^T(=KCTC 25386^T = VKM B-3628^T = UQM 41477^T).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 2","pages":"Article 126591"},"PeriodicalIF":3.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavobacterium plantiphilum sp. nov., Flavobacterium rhizophilum sp. nov., Flavobacterium rhizosphaerae sp. nov., Chryseobacterium terrae sp. nov., and Sphingomonas plantiphila sp. nov. isolated from salty soil showing plant growth promoting potential","authors":"Peter Kämpfer ,&nbsp;André Lipski ,&nbsp;Kathy S. Lawrence ,&nbsp;Walker R. Olive ,&nbsp;Molli M. Newman ,&nbsp;John A. McInroy ,&nbsp;Tomeu Viver","doi":"10.1016/j.syapm.2025.126588","DOIUrl":"10.1016/j.syapm.2025.126588","url":null,"abstract":"<div><div>Members of the genera <em>Flavobacterium</em>, <em>Chryseobacterium</em> and <em>Sphingomonas</em> constitute a group of microorganisms in the rhizosphere associated with plant growth promoting (PGP) features. A polyphasic approach was employed to ascertain the taxonomic status of five selected strains. Overall genome relatedness indices of digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) between the strains and the other members of the genera <em>Flavobacterium</em>, <em>Chryseobacterium</em> and <em>Sphingomonas</em> were found to be below the established thresholds, respectively. Morphological, physiological, and biochemical characteristics of the strains confirmed their status as five novel species. A large variety of genes involved in plant growth promotion and carbohydrate utilization were found in all strains suggesting a contribution of all strains to PGP.</div><div>Based on the result of the polyphasic characterization, the following names are proposed: <em>Chryseobacterium terrae</em> sp. nov., with the strain ST-37^T as the type strain (= CCM 9260^T = LMG 32728^T); <em>Flavobacterium plantiphilum</em> sp. nov., with the strain ST-87^T as the type strain CIP 112180^T = DSM 114790^T = LMG 32757^T); <em>Flavobacterium rhizophilum</em> sp. nov., with the strain ST-75^T as the type strain (= CIP 112185^T = DSM 114831^T = LMG 32758^T); <em>Flavobacterium rhizosphaerae</em> sp. nov., with the strain ST-119^T as the type strain (CIP 112181^T = DSM 114832^T = LMG 32756^T); and <em>Sphingomonas plantiphila</em> sp. nov. with the strain ST-64 ^T as the type strain (= CCM 9261^T = CIP 112178^T = DSM 114515^T = LMG 32729^T).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 2","pages":"Article 126588"},"PeriodicalIF":3.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic description and genome sequence of Anaerorudis cellulosivorans gen. Nov. sp. nov., a novel cellulose- and Xylan-degrading bacterium of the Bacteroidota phylum isolated from a lab-scale methanogenic landfill bioreactor digesting municipal solid waste","authors":"Abdelaziz El Houari ,&nbsp;Morgan Carpenter ,&nbsp;Daniel Chaplin ,&nbsp;Peter Golyshin ,&nbsp;James E. McDonald","doi":"10.1016/j.syapm.2025.126590","DOIUrl":"10.1016/j.syapm.2025.126590","url":null,"abstract":"<div><div>Bacteria responsible for the anaerobic decomposition of lignocellulosic waste biomass play key roles in the global carbon cycle and possess enzymes with potential industrial application. Here, a novel anaerobic, thermophilic, non-spore-forming bacterium, strain m5^T, was isolated from methanogenic enrichment cultures obtained from a lab-scale methanogenic landfill bioreactor digesting anaerobic municipal solid waste. Cells were Gram-stain-negative, catalase-negative, oxidase-negative, rod-shaped, and non-motile. The genomic DNA G + C content was 40.92 mol%. The optimal NaCl concentration, temperature and pH for growth were 0.5–1 g.L⁻¹, 45 °C, and at pH 7.0, respectively. The major fatty acids were C_14:0, C_16:0, C_18:0, C_18:1 <em>ω</em>9<em>c</em>, and anteisoC_15:0. Strain m5^T was able to grow in the absence of yeast extract on glucose, fructose, arabinose, cellobiose, galactose, maltose, raffinose, sucrose, lactose, and pyruvate. In the presence of 0.2 % yeast extract, strain m5^T grew on wide range of carbohydrates and amino acids, and was able to use complex substrates such cellulose and xylan. Major end products from cellulose and xylan degradation were valerate and propionate. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate was most closely related to <em>Seramator thermalis</em> SYSU GA16112^T (94.42 % 16S rRNA gene sequence identity). Genome-based relatedness as well as both Average Nucleotide Identity (ANI), and Average Amino Acid Identity (AAI) strongly supported that strain m5^T belongs to the <em>Dysgonomonadaceae</em> family. Metagenomic analysis of the landfill bioreactor community revealed that the <em>Dysgonomonadaceae</em> family was the most abundant in the constructed bioreactors. Based on its unique genomic features, strain m5^T is considered to represent a novel genus, for which the name <em>Anaerorudis</em> is proposed. Moreover, several phenotypic, biochemical, and physiological properties differentiated the novel bacterial strain from related species, indicating that the strain represents a new species for which the name <em>Anaerorudis cellulosivorans</em> sp. nov. is proposed, with strain m5^T (= DSM 112743^T = ATCC TSD-267^T) being the type of strain. This study highlights the biotechnological potential of strain m5^T, specifically in the bioconversion of cellulose and xylan, a recalcitrant substrate within lignocellulosic plant biomass, to enhance biogas production.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 2","pages":"Article 126590"},"PeriodicalIF":3.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description and genomic characterization of Mesorhizobium marinum sp. nov., a bacterium isolated from sea sediment","authors":"Chengshao Zan ,&nbsp;Xiao Ma ,&nbsp;Zhouqing Zheng ,&nbsp;Feina Li ,&nbsp;Xian Yang ,&nbsp;Yifen Luo ,&nbsp;Li Tuo","doi":"10.1016/j.syapm.2025.126589","DOIUrl":"10.1016/j.syapm.2025.126589","url":null,"abstract":"<div><div>Two Gram-stain-negative, aerobic strains, designed ZMM04-4^T and ZMM04–5, were isolated from sea sediment collected from Qinzhou Bay, Guangxi Zhuang Automous Region, China. The predominant respiratory quinone was ubiquinone-10. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, aminophospholipid and phospholipid. The predominant fatty acids were C_18:1<em>ω</em>7c, C_19:0 cyclo <em>ω</em>8c and C_16:0. Strain ZMM04-4^T shared 99.7 % similarity of 16S rRNA gene sequence with ZMM04–5. The digital DNA-DNA hybridization and average nucleotide identity values between strain ZMM04-4^T and ZMM04–5 were 92.9 % and 98.6 %, respectively, indicating that strains ZMM04-4^T, ZMM04–5 belong to the same species. Phylogenetic and phylogenomic analysis indicated that strains ZMM04-4^T and ZMM04–5 belong to the genus <em>Mesorhizobium</em> and showed the highest sequence similarity to <em>Mesorhizobium qingshengii</em> CGMCC 1.12097^T (97.7 %–97.8 % sequence similarity) and <em>Mesorhizobium shangrilense</em> DSM 21850^T (97.7 %–97.8 %). The average nucleotide identity and digital DNA-DNA hybridization values between strains ZMM04-4^T, ZMM04–5 and their closely related species were within the ranges of 76.5 %–77.2 % and 20.9 %–21.8 %, respectively, indicating that strains ZMM04-4^T, ZMM04–5 were novel species. In accordance with phylogenetic and genomic as well as phenotypic and chemotaxonomic characterizations, strains ZMM04-4^T and ZMM04–5 should be assigned to the genus <em>Mesorhizobium</em> and indentified as a novel species, for which the name <em>Mesorhizobium marinum</em> sp. nov., is proposed. The type strain is ZMM04-4^T (=MCCC 1K08883^T = KCTC 8273^T).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 2","pages":"Article 126589"},"PeriodicalIF":3.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MIRRI-ERIC's position on the recent evolution of the international code of nomenclature of prokaryotes","authors":"Rosa Aznar ,&nbsp;Michel-Yves Mistou ,&nbsp;Praveen Rahi ,&nbsp;Jean-Luc Legras ,&nbsp;Anete Boroduske ,&nbsp;Amalia Stefaniu ,&nbsp;Nelson Lima ,&nbsp;Amalia D. Karagouni ,&nbsp;Vincent Van de Perre ,&nbsp;Ana M.P. Melo","doi":"10.1016/j.syapm.2025.126587","DOIUrl":"10.1016/j.syapm.2025.126587","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 2","pages":"Article 126587"},"PeriodicalIF":3.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}