Rosettibacter primus gen. nov., sp. nov., and Rosettibacter firmus sp. nov., facultatively anaerobic moderately thermophilic bacteria of the class Ignavibacteria from hot springs of North Ossetia

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Olga A. Podosokorskaya, Nika F. Petrova, Ekaterina N. Tikhonova, Alexandra A. Klyukina, Alexander G. Elcheninov
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引用次数: 0

Abstract

A novel facultatively anaerobic moderately thermophilic bacteria, strains 4137-MeT and 4148-MeT, were isolated from hot springs of Karmadon and Ursdon, respectively (North Ossetia, Russian Federation). Gram-negative, motile rods were present singly, in pairs, rosettes, and aggregates, or formed biofilms. Both strains grew optimally at 50–55 °C, pH 7.0 and did not require sodium chloride or yeast extract for growth. They were chemoorganoheterotrophs, growing on mono-, di- and polysaccharides (cellulose, starch, xylan, lichenan, galactan, xyloglucan, mannan, xanthan gum, guar gum) as well as proteinaceous substrates (gelatin, peptone, beef and yeast extract). Growth under anaerobic conditions was observed in presence and absence of external electron acceptors. Sulfur, thiosulfate, arsenate, Fe-citrate, and ferrihydrite were reduced with acetate, starch, or yeast extract as electron donors. The respiratory quinone was MK-7. Major cellular fatty acids of both strains were iso-C15:0, anteiso-C17:0, C15:0, iso-C16:0 and additionally iso-C17:0 for strain 4137-MeT. The size of the genome and genomic DNA G + C content of strain 4137-MeT were 3.24 Mb. and 29.9 %, respectively; for strain 4148-MeT – 3.33 Mb and 30.7 %. According to the 16S rRNA gene sequence and conserved protein sequences phylogenies, strains 4137-MeT and 4148-MeT represented a distinct lineage of the family Melioribacteraceae within the class Ignavibacteria. Based on phylogenetic analysis and phenotypic features, the novel isolates were assigned to a novel genus, for which the name Rosettibacter gen. nov. is proposed. Strain 4148-MeT represents its type species Rosettibacter primus sp. nov., while strain 4137-MeT represents a new species Rosettibacter firmus sp. nov.

Rosettibacter primus gen.
一种新型兼性厌氧中度嗜热细菌--菌株 4137-MeT 和 4148-MeT--分别从卡尔马东和乌尔斯顿(俄罗斯联邦北奥塞梯)的温泉中分离出来。这两种菌株均为革兰氏阴性,可运动,以单个、成对、莲座状和聚集状存在,或形成生物膜。这两种菌株在 50-55 °C、pH 值为 7.0 的条件下生长最佳,生长不需要氯化钠或酵母提取物。它们是化学有机异养生物,可在单糖、双糖和多糖(纤维素、淀粉、木聚糖、地衣聚糖、半乳聚糖、木聚糖、甘露聚糖、黄原胶、瓜尔胶)以及蛋白质基质(明胶、蛋白胨、牛肉和酵母提取物)上生长。在有和没有外部电子受体的情况下,都能观察到厌氧条件下的生长情况。以醋酸盐、淀粉或酵母提取物为电子供体还原了硫、硫代硫酸盐、砷酸盐、柠檬酸铁和亚铁。呼吸醌为 MK-7。两种菌株的主要细胞脂肪酸均为异-C15:0、前-C17:0、C15:0、异-C16:0,4137-MeT 菌株的主要细胞脂肪酸为异-C17:0。菌株 4137-MeT 的基因组大小和基因组 DNA G + C 含量分别为 3.24 Mb 和 29.9 %;菌株 4148-MeT 的基因组大小和基因组 DNA G + C 含量分别为 3.33 Mb 和 30.7 %。根据 16S rRNA 基因序列和保守蛋白序列的系统发育,4137-MeT 和 4148-MeT 菌株代表了伊格纳维氏菌(Ignavibacteria)中美利奥氏菌科(Melioribacteraceae)的一个独特品系。根据系统进化分析和表型特征,这两个新分离株被归入一个新属,命名为 Rosettibacter gen.菌株 4148-MeT 代表其模式种 Rosettibacter primus sp.
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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