Systematic and applied microbiology最新文献

{"title":"Corrigendum to: \"Flavobacterium plantiphilum sp. nov., Flavobacterium rhizophilum sp. nov., Flavobacterium rhizosphaerae sp. nov., Chryseobacterium terrae sp. nov., and Sphingomonas plantiphila sp. nov. isolated from salty soil showing plant growth promoting potential\" [Systematic and Applied Microbiology 48 (2025) 126588].","authors":"Peter Kämpfer, André Lipski, Kathy S Lawrence, Walker R Olive, Molli M Newman, John A McInroy, Tomeu Viver","doi":"10.1016/j.syapm.2025.126603","DOIUrl":"https://doi.org/10.1016/j.syapm.2025.126603","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":" ","pages":"126603"},"PeriodicalIF":3.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and metabolic characterisation of a novel species Magnetominusculus dajiuhuensis DJH-1Ts sp. nov. from an acidic peatland","authors":"Pranami Goswami ,&nbsp;Runjia Ji ,&nbsp;Jianxun Shen ,&nbsp;Andrew P. Roberts ,&nbsp;Wei Lin","doi":"10.1016/j.syapm.2025.126605","DOIUrl":"10.1016/j.syapm.2025.126605","url":null,"abstract":"<div><div>Magnetotactic bacteria (MTB) are recognised widely for their ability to synthesise intracellular magnetite (Fe₃O₄) and/or greigite (Fe₃S₄) nanocrystals and align with Earth's magnetic field. They are crucial for understanding prokaryotic organelle biogenesis. MTB members of the <em>Nitrospirota</em> phylum (previously known as the <em>Nitrospirae</em> phylum) are of interest due to their important ecological roles in the biogeochemical cycling of iron and sulphur. Here, we introduce <em>Magnetominusculus dajiuhuensis</em> DJH-1^Ts, a newly discovered <em>Nitrospirota</em> MTB species that thrives in the acidic Dajiuhu Peatland of central China. By combining electron microscopy, 16S rRNA gene-based analysis and genome-resolved metagenomics, we elucidate its distinctive morphology, genomic features, and metabolic functions. The metagenome-assembled genome, assigned to the genus <em>Magnetominusculus</em>, family <em>Magnetobacteriaceae</em>, order <em>Thermodesulfovibrionales</em>, class <em>Thermodesulfovibrionia</em> according to the GTDB taxonomy, reveals an obligate anaerobe that lives in central China's largest wetland. We propose the formal name <em>Magnetominusculus dajiuhuensis</em> DJH-1^Ts sp. nov., following the SeqCode system. Genomic and metabolic characterisation of this novel species suggests its potential role in nitrogen, sulphur, and carbon metabolism in aquatic biogeochemistry, particularly in peatlands. The genome of this novel strain indicates that it harnesses the Wood-Ljungdahl pathway for carbon fixation and acetate metabolism in anaerobic conditions, while its potential role in nitrogen cycling is characterised by denitrification and nitrogen fixation. It also participates in reduction of sulphate to sulphide, indicating a role in sulphur cycling in its ecological niche. Taken together, the discovery and characterisation of <em>Magnetominusculus dajiuhuensis</em> DJH-1^Ts provide new insights into MTB diversity and ecological functions, particularly in peatland biogeochemistry.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126605"},"PeriodicalIF":3.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel thermophilic hydrolytic bacterium Rarispira pelagica gen. nov., sp. nov., reclassification of Spirochaeta thermophila as Winmispira thermophila gen. nov., comb. nov., and proposal of Winmispiraceae fam. nov. and Winmispirales ord. nov. in the class Spirochaetia","authors":"Olga A. Podosokorskaya ,&nbsp;Anastasiia I. Maltseva ,&nbsp;Alexander G. Elcheninov ,&nbsp;Andrei A. Novikov ,&nbsp;Kseniya S. Zayulina ,&nbsp;Alexander Y. Merkel","doi":"10.1016/j.syapm.2025.126604","DOIUrl":"10.1016/j.syapm.2025.126604","url":null,"abstract":"<div><div>A novel anaerobic moderately thermophilic bacterium, strain 38H-sp^T, was isolated from a hot spring located 12 m deep of the Kunashir Island shore. Gram-stain negative cells were non-sporeforming, motile, thin helices with regular coiling, occasionally forming bundles of cells. The strain grew at 30–60 °C and pH range of 5.5–8.4 with an optimum at 55 °C and pH 6.6–7.0. Strain 38H-sp^T required 0.5–5.5% NaCl (1.5% is an optimum) for growth. It was a chemoorganoheterotroph, growing on carbohydrates, including polymers (starch, pullulan, xylan, cellulose, arabinoxylan, xanthan gum, mannan, galactomannan, alginate and laminarin). Major products of glucose fermentation were acetate, ethanol, hydrogen, and carbon dioxide. Major cellular fatty acids were iso-C_15:0, C_14:0, C_12:1 DMA. The size of complete genome of strain 38H-sp^T was 2.35 Mb; DNA G + C content was 40.1%. 93 CAZymes including 54 glycoside hydrolases were found to be encoded in the genome of the strain. According to 16S rRNA gene sequence and conserved protein sequences phylogenies strain 38H-sp^T with its closest relative <em>Spirochaeta thermophila</em> represented a deeply branched lineage of the class <em>Spirochaetia.</em> Based on phylogenetic analysis and phenotypic features these two bacteria were assigned to a novel family within a novel order for that the names <em>Winmispiraceae</em> fam. nov. and <em>Winmispirales</em> ord. nov. are proposed with <em>Winmispira thermophila</em> gen. nov., comb. nov. (previously known as <em>Spirochaeta thermophila</em>) as type species. Strain 38H-sp^T (=DSM 100344^T = VKM B-2965^T) represents the novel genus and species <em>Rarispira pelagica</em> gen. nov., sp. nov.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126604"},"PeriodicalIF":3.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143682041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thiohalorhabdus methylotropha sp. nov., an extremely halophilic autotrophic methylotiotroph from hypersaline lakes","authors":"Dimitry Y. Sorokin ,&nbsp;Alexander Y. Merkel ,&nbsp;William Gebbe ,&nbsp;Marina G. Kalyuzhnaya","doi":"10.1016/j.syapm.2025.126602","DOIUrl":"10.1016/j.syapm.2025.126602","url":null,"abstract":"<div><div>So far, there have been no reports of trimethylamine (TMA)-utilizing extremely halophilic microorganisms in hypersaline habitats. Our aerobic enrichments at 4 M total Na⁺ with 5 mM TMA inoculated with surface sediments from hypersaline soda (at pH 9.5) or chloride-sulfate (at pH 7) lakes in southwestern Siberia were successful only for the latter. The initial enrichment included both bacteria and haloarchaea but only the bacterial component was able to grow as a pure culture with TMA. Strain Cl-TMA forms a new-species lineage within the genus <em>Thiohalorhabdus</em> which includes extremely halophilic and obligate lithoautotrophic sulfur-oxidizing gammaproteobacteria. Cl-TMA can grow methyloautotrophically utilizing TMA, dimethylamine (DMA) and methanol (MeOH) as the electron donors or chemolithoautotrophically with thiosulfate. Mixotrophic growth was also observed with the three methyl compounds and thiosulfate. Carbon is assimilated autotrophically via the Calvin-Benson-Basham pathway. Unlike the type species of <em>Thiohalorhabdus</em>, <em>T. denitrificans</em>, Cl-TMA was incapable of anaerobic growth via denitrification. The isolate belongs to extreme halophiles growing between 2.5 and 5 M NaCl with an optimum at 3–3.5 M. Genome analysis identified two gene clusters coding for PQQ-dependent methanol dehydrogenases (MxaFI and XoxF), four homologues of the formaldehyde activating enzymes (Faes), a TMA/DMA oxidation locus, and two cluster of genes encoding an <em>N</em>-methylglutamate dehydrogenase pathway (NMGP) for methylamine oxidation. The first steps of C₁-subtrate conversions are followed by the tetrahydrofolate (THF)-linked and tetrahydromethanopterin (H4MPT)-linked formaldehyde oxidation pathways and two formate dehydrogenases. All of those signatures of methylotrophy were absent in <em>T. denitrificans</em>. In contrast, genes for two key sulfur oxidation enzymes, thiosulfate dehydrogenase TsdAB and sulfide dehydrogenase FccAB, that are present in the type species are missing in Cl-TMA. Thiosulfate is oxidized to sulfate by a combination of an incomplete Sox cycle and an sHdr system. Strain Cl-TMA^T (JCM 35977 = UQM 41915) is proposed to be classified as <em>Thiohalorhabdus methylotrophus</em> sp. nov.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126602"},"PeriodicalIF":3.3,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to \"Characterization of two novel species of the genusBifidobacterium: Bifidobacterium saimiriisciurei sp. nov. and Bifidobacterium platyrrhinorum sp. nov.\" [Syst. Appl. Microbiol. 43 (2020) 126111].","authors":"Monica Modesto, Maria Satti, Koichi Watanabe, Donatella Scarafile, Chien-Hsun Huang, Jong-Shian Liou, Tomohiko Tamura, Satomi Saito, Mizuki Watanabe, Koji Mori, Lina Huang, Camillo Sandri, Caterina Spiezio, Masanori Arita, Paola Mattarelli","doi":"10.1016/j.syapm.2025.126601","DOIUrl":"https://doi.org/10.1016/j.syapm.2025.126601","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":" ","pages":"126601"},"PeriodicalIF":3.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three new Microbacterium species isolated from the Marmara Sea mucilage event: Microbacterium istanbulense sp. nov., Microbacterium bandirmense sp. nov., Microbacterium marmarense sp. nov","authors":"Izzet Burcin Saticioglu ,&nbsp;Nihed Ajmi ,&nbsp;Orkid Coskuner-Weber ,&nbsp;Semih Alpsoy ,&nbsp;Hilal Ay ,&nbsp;Fuat Aydin ,&nbsp;Seçil Abay ,&nbsp;Emre Karakaya ,&nbsp;Tuba Kayman ,&nbsp;Cem Dalyan ,&nbsp;Fatih Doğan Koca ,&nbsp;Gorkem Tasci ,&nbsp;Doğancan Yarim ,&nbsp;Danny Morick ,&nbsp;Artun Yibar ,&nbsp;Serdar Erdogan ,&nbsp;Soner Altun ,&nbsp;Muhammed Duman","doi":"10.1016/j.syapm.2025.126600","DOIUrl":"10.1016/j.syapm.2025.126600","url":null,"abstract":"<div><div>Three bacterial strains, Mu-43^T, Mu-80^T, and Mu-86^T, were isolated from the 2021 and 2022 mucilage event in the Marmara Sea and were taxonomically characterized. 16S rRNA gene sequence analysis confirmed that these strains belong to the genus <em>Microbacterium</em>. A polyphasic approach involving genomic and phenotypic analysis was employed to determine their taxonomic positions. A polyphasic approach integrating genomic and phenotypic analyses established their taxonomic positions. <em>M. istanbulense</em> Mu-43^T showed 99.0 % 16S rRNA similarity to <em>M. bandirmense</em> Mu-80^T, with digital DNA–DNA hybridization (dDDH) and average nucleotide identity using BLAST (ANIb) values of 22.3 % and 78.3 %, respectively. <em>M. bandirmense</em> Mu-80^T exhibited 99.2 % similarity to <em>M. esteraromaticum</em> DSM 8609^T, with dDDH and ANIb values of 23.6 % and 80 %. <em>M. marmarense</em> Mu-86^T showed 97.4 % similarity to <em>M. arthrosphaerae</em> JCM 30492^T, with dDDH and ANIb values of 20.1 % and 74.2 %. Metagenomic analysis highlighted their ecological relevance, with relative abundances of 1.43 %, 1.15 %, and 0.95 %, respectively. Further genomic analysis identified biosynthetic gene clusters associated with secondary metabolite production, including non-ribosomal peptide synthetases and terpenoid biosynthesis pathways, suggesting potential antimicrobial activity. Additionally, antibiotic resistance genes, such as ABC efflux pumps and Erm23S_rRNA methyltransferase, indicate adaptation to environmental stress. These findings indicate that these species contribute to nutrient cycling and organic matter decomposition in mucilage-affected environments. Based on genomic and phenotypic data, these strains are proposed as novel species: <em>M. istanbulense</em> sp. nov. Mu-43^T (LMG 33297^T = DSM 117065^T), <em>M. bandirmense</em> sp. nov. Mu-80^T (LMG 33295^T = DSM 117210^T), and <em>M. marmarense</em> sp. nov. Mu-86^T (LMG 33293^T = DSM 117066^T).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126600"},"PeriodicalIF":3.3,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based taxonomy of the family Haloarculaceae, proposal of Natronomonadaceae fam. nov., and description of four novel halophilic archaea from two saline lakes and a marine solar saltern","authors":"Ling-Rui Zhu,&nbsp;Ya-Ling Mao,&nbsp;Yao Hu,&nbsp;Ya-Ping Sun,&nbsp;Jing Hou,&nbsp;Heng-Lin Cui","doi":"10.1016/j.syapm.2025.126592","DOIUrl":"10.1016/j.syapm.2025.126592","url":null,"abstract":"<div><div>A new family related to the family <em>Haloarculaceae</em> was proposed and the genus <em>Actinarchaeum</em> was merged into the genus <em>Halocatena</em> through phylogenetic, phylogenomic, and comparative genomic analyses. Four strains KK48^T, YCN56^T, SYNS191^T, and SYNS196^T with new taxonomic status were isolated from inland saline lakes and a marine solar saltern. According to the comparison of 16S rRNA gene and <em>rpoB'</em> gene sequences, strains KK48^T, YCN56^T, SYNS191^T, and SYNS196^T showed high sequence similarities to the genera <em>Salinibaculum</em> and <em>Salinirubellus</em>, respectively. The values of average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity between these strains and the species of <em>Salinibaculum</em> and <em>Salinirubellus</em> ranged from 75.3 to 77.7 %, 24.5–25.9 % and 66.3–73.4 %, respectively. These data were well below the threshold for species classification, supporting their placements in new taxa. The major polar lipids of these strains were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glucosyl diether, mannosyl glucosyl diether, and disulfated mannosyl glucosyl diether. Based on the phenotypic, chemotaxonomic, phylogenetic, and phylogenomic properties, strains KK48^T (= CGMCC 1.19060^T = JCM 35607^T), YCN56^T (= CGMCC 1.62603^T = JCM 36493^T), SYNS191^T (= CGMCC 1.62607^T = JCM 36494^T), and SYNS196^T (= CGMCC 1.62608^T = JCM 36495^T) represent four novel species of the genera <em>Salinibaculum</em> and <em>Salinirubellus</em>. And <em>Salinibaculum rarum</em> sp. nov., <em>Salinibaculum salinum</em> sp. nov., <em>Salinibaculum marinum</em> sp. nov., and <em>Salinirubellus litoreus</em> sp. nov. are proposed to accommodate these strains.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 3","pages":"Article 126592"},"PeriodicalIF":3.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143534782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tenuifilum osseticum sp. nov., a novel thermophilic hydrolytic bacterium within the Tenuifilaceae isolated from a North Ossetian thermal spring, and emended description of the genus Tenuifilum","authors":"Olga A. Podosokorskaya ,&nbsp;Maria I. Prokofeva ,&nbsp;Alexandra A. Kuchierskaya ,&nbsp;Alexandra A. Klyukina ,&nbsp;Alexander G. Elcheninov","doi":"10.1016/j.syapm.2025.126591","DOIUrl":"10.1016/j.syapm.2025.126591","url":null,"abstract":"<div><div>A novel anaerobic moderately thermophilic bacterium, strain 4138-str^T, was isolated from a thermal spring of North Ossetia (Russian Federation). Gram-negative cells were non-sporeforming, straight or curved filamentous rods, occasionally forming rosettes. The strain grew at 30–55 °C, pH range of 6.1–8.7, NaCl range of 0–4 %, with an optimum at 50 °C, pH 7.1–7.5 and 0.2–0.4 % NaCl. It was a chemoorganoheterotroph, growing on simple sugars (glucose, maltose, cellobiose, etc.) and carbohydrates (starch, pullulan, laminarin, xylan, lichenan, curdlan, pachyman) or proteinaceous substrates (peptone, tryptone, gelatin, casein). Sulfur was used as electron acceptor. Major products of glucose fermentation were acetate, hydrogen, and carbon dioxide. Major cellular fatty acids were iso-C_15:0 and anteiso-C_15:0. The quinone was MK-7. The size of the whole genome of strain 4138-str^T was 3.275 Mbp; DNA G + C content was 42.1 %. Genome analysis allowed to identify genes encoding carbohydrate-active enzymes and extracellular proteases. In addition, central metabolism and fermentation pathways of strain 4138-str^T were reconstructed. According to both phylogenetic analyses, based on 16S rRNA gene sequences and conserved protein sequences, as well as genome-based comparisons, strain 4138-str^T formed a species-level lineage within <em>Tenuifilum</em> genus of <em>Tenuifilaceae</em> family (phylum <em>Bacteroidota</em>). Here we propose a novel species <em>Tenuifilum osseticum</em> sp. nov. with type strain 4138-str^T(=KCTC 25386^T = VKM B-3628^T = UQM 41477^T).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 2","pages":"Article 126591"},"PeriodicalIF":3.3,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic description and genome sequence of Anaerorudis cellulosivorans gen. Nov. sp. nov., a novel cellulose- and Xylan-degrading bacterium of the Bacteroidota phylum isolated from a lab-scale methanogenic landfill bioreactor digesting municipal solid waste","authors":"Abdelaziz El Houari ,&nbsp;Morgan Carpenter ,&nbsp;Daniel Chaplin ,&nbsp;Peter Golyshin ,&nbsp;James E. McDonald","doi":"10.1016/j.syapm.2025.126590","DOIUrl":"10.1016/j.syapm.2025.126590","url":null,"abstract":"<div><div>Bacteria responsible for the anaerobic decomposition of lignocellulosic waste biomass play key roles in the global carbon cycle and possess enzymes with potential industrial application. Here, a novel anaerobic, thermophilic, non-spore-forming bacterium, strain m5^T, was isolated from methanogenic enrichment cultures obtained from a lab-scale methanogenic landfill bioreactor digesting anaerobic municipal solid waste. Cells were Gram-stain-negative, catalase-negative, oxidase-negative, rod-shaped, and non-motile. The genomic DNA G + C content was 40.92 mol%. The optimal NaCl concentration, temperature and pH for growth were 0.5–1 g.L⁻¹, 45 °C, and at pH 7.0, respectively. The major fatty acids were C_14:0, C_16:0, C_18:0, C_18:1 <em>ω</em>9<em>c</em>, and anteisoC_15:0. Strain m5^T was able to grow in the absence of yeast extract on glucose, fructose, arabinose, cellobiose, galactose, maltose, raffinose, sucrose, lactose, and pyruvate. In the presence of 0.2 % yeast extract, strain m5^T grew on wide range of carbohydrates and amino acids, and was able to use complex substrates such cellulose and xylan. Major end products from cellulose and xylan degradation were valerate and propionate. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate was most closely related to <em>Seramator thermalis</em> SYSU GA16112^T (94.42 % 16S rRNA gene sequence identity). Genome-based relatedness as well as both Average Nucleotide Identity (ANI), and Average Amino Acid Identity (AAI) strongly supported that strain m5^T belongs to the <em>Dysgonomonadaceae</em> family. Metagenomic analysis of the landfill bioreactor community revealed that the <em>Dysgonomonadaceae</em> family was the most abundant in the constructed bioreactors. Based on its unique genomic features, strain m5^T is considered to represent a novel genus, for which the name <em>Anaerorudis</em> is proposed. Moreover, several phenotypic, biochemical, and physiological properties differentiated the novel bacterial strain from related species, indicating that the strain represents a new species for which the name <em>Anaerorudis cellulosivorans</em> sp. nov. is proposed, with strain m5^T (= DSM 112743^T = ATCC TSD-267^T) being the type of strain. This study highlights the biotechnological potential of strain m5^T, specifically in the bioconversion of cellulose and xylan, a recalcitrant substrate within lignocellulosic plant biomass, to enhance biogas production.</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 2","pages":"Article 126590"},"PeriodicalIF":3.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description and genomic characterization of Mesorhizobium marinum sp. nov., a bacterium isolated from sea sediment","authors":"Chengshao Zan ,&nbsp;Xiao Ma ,&nbsp;Zhouqing Zheng ,&nbsp;Feina Li ,&nbsp;Xian Yang ,&nbsp;Yifen Luo ,&nbsp;Li Tuo","doi":"10.1016/j.syapm.2025.126589","DOIUrl":"10.1016/j.syapm.2025.126589","url":null,"abstract":"<div><div>Two Gram-stain-negative, aerobic strains, designed ZMM04-4^T and ZMM04–5, were isolated from sea sediment collected from Qinzhou Bay, Guangxi Zhuang Automous Region, China. The predominant respiratory quinone was ubiquinone-10. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, aminophospholipid and phospholipid. The predominant fatty acids were C_18:1<em>ω</em>7c, C_19:0 cyclo <em>ω</em>8c and C_16:0. Strain ZMM04-4^T shared 99.7 % similarity of 16S rRNA gene sequence with ZMM04–5. The digital DNA-DNA hybridization and average nucleotide identity values between strain ZMM04-4^T and ZMM04–5 were 92.9 % and 98.6 %, respectively, indicating that strains ZMM04-4^T, ZMM04–5 belong to the same species. Phylogenetic and phylogenomic analysis indicated that strains ZMM04-4^T and ZMM04–5 belong to the genus <em>Mesorhizobium</em> and showed the highest sequence similarity to <em>Mesorhizobium qingshengii</em> CGMCC 1.12097^T (97.7 %–97.8 % sequence similarity) and <em>Mesorhizobium shangrilense</em> DSM 21850^T (97.7 %–97.8 %). The average nucleotide identity and digital DNA-DNA hybridization values between strains ZMM04-4^T, ZMM04–5 and their closely related species were within the ranges of 76.5 %–77.2 % and 20.9 %–21.8 %, respectively, indicating that strains ZMM04-4^T, ZMM04–5 were novel species. In accordance with phylogenetic and genomic as well as phenotypic and chemotaxonomic characterizations, strains ZMM04-4^T and ZMM04–5 should be assigned to the genus <em>Mesorhizobium</em> and indentified as a novel species, for which the name <em>Mesorhizobium marinum</em> sp. nov., is proposed. The type strain is ZMM04-4^T (=MCCC 1K08883^T = KCTC 8273^T).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 2","pages":"Article 126589"},"PeriodicalIF":3.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}