Systematic and applied microbiology最新文献

{"title":"Corynebacterium antarcticum sp. nov., Corynebacterium marambiense sp. nov., Corynebacterium meridianum sp. nov., and Corynebacterium pygosceleis sp. nov., isolated from Adélie penguins (Pygoscelis adeliae)","authors":"Pavel Švec ,&nbsp;Hans-Jürgen Busse ,&nbsp;Karel Sedlář ,&nbsp;Jana Musilová ,&nbsp;Stanislava Králová ,&nbsp;Eva Staňková ,&nbsp;Ondrej Šedo ,&nbsp;Daniel Krsek ,&nbsp;Vendula Koublová ,&nbsp;Lucie Krištofová ,&nbsp;Ivo Sedláček","doi":"10.1016/j.syapm.2022.126390","DOIUrl":"10.1016/j.syapm.2022.126390","url":null,"abstract":"<div><p><span>A taxonomic study was conducted on 16 bacterial strains isolated from wild Adélie penguins (</span><span><em>Pygoscelis adeliae</em></span><span>) from Seymour (Marambio) Island and James Ross Island. An initial screening by repetitive sequence-based PCR fingerprinting divided the strains studied into four coherent groups. Phylogenetic analysis based on 16S rRNA gene sequences assigned all groups to the genus </span><span><em>Corynebacterium</em></span> and showed that <em>Corynebacterium glyciniphilum</em> and <em>Corynebacterium terpenotabidum</em><span> were the closest species with 16S rRNA gene sequence similarities between 95.4 % and 96.5 %. Further examination of the strains studied with ribotyping<span>, MALDI-TOF mass spectrometry, comprehensive biotyping and calculation of average nucleotide identity and digital DNA–DNA hybridisation values confirmed the separation of the four groups from each other and from the other </span></span><em>Corynebacterium</em> species. Chemotaxonomically, the four strains P5828^T, P5850^T, P6136^T, P7210^T representing the studied groups were characterised by C_16:0 and C_18:1 <em>ω9c</em> as the major fatty acids, by the presence of <em>meso</em><span>-diaminopimelic acid in the peptidoglycan<span>, the presence of corynemycolic acids and a quinone<span> system with the predominant menaquinone MK-9(H</span></span></span>₂). The results of this study show that the strains studied represent four new species of the genus <em>Corynebacterium</em>, for which the names <span><em>Corynebacterium </em><em>antarcticum</em></span> sp. nov. (type strain P5850^T = CCM 8835^T = LMG 30620^T), <em>Corynebacterium marambiense</em> sp. nov. (type strain P5828^T = CCM 8864^T = LMG 31626^T), <em>Corynebacterium meridianum</em> sp. nov<strong>.</strong> (type strain P6136^T = CCM 8863^T = LMG 31628^T) and <em>Corynebacterium pygosceleis</em> sp. nov. (type strain P7210^T = CCM 8836^T = LMG 30621^T) are proposed.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10588493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic proposal for a deep branching bacterial phylogenetic lineage: transfer of the family Thermodesulfobiaceae to Thermodesulfobiales ord. nov., Thermodesulfobiia classis nov. and Thermodesulfobiota phyl. nov","authors":"Evgenii N. Frolov,&nbsp;Alexander V. Lebedinsky,&nbsp;Alexander G. Elcheninov,&nbsp;Ilya V. Kublanov","doi":"10.1016/j.syapm.2022.126388","DOIUrl":"10.1016/j.syapm.2022.126388","url":null,"abstract":"<div><p>The family <em>Thermodesulfobiaceae</em>, comprising one genus <em>Thermodesulfobium</em> with two validly published species, is currently assigned to order <em>Thermoanaerobacterales</em> within the class <span><em>Clostridia</em></span> of the phylum <em>Bacillota</em><span>. At the same time, the very first 16S rRNA gene sequence-based phylogenetic studies of representatives of the genus pointed out great differences between </span><em>Thermodesulfobium</em> and other members of the phylum <em>Bacillota</em>. Subsequent studies of new <em>Thermodesulfobium</em><span> representatives supported deep phylogenetic branching of this lineage<span> within bacterial tree, implying that it represents a novel phylum. The results of the phylogenomic analysis performed in the frames of the present work confirm previous findings and suggest that </span></span><em>Thermodesulfobium</em> represents a distinct phylum-level lineage. Thus, we propose the transfer of the family <em>Thermodesulfobiaceae</em> to the new order <em>Thermodesulfobiales</em> within the new class <em>Thermodesulfobiia</em> and the new phylum <em>Thermodesulfobiota</em>.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10533635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of five novel thermophilic species of the genus Thermus: Thermus hydrothermalis sp. nov., Thermus neutrinimicus sp. nov., Thermus thalpophilus sp. nov., Thermus albus sp. nov., and Thermus altitudinis sp. nov., isolated from hot spring sediments","authors":"Meng-Meng Li ,&nbsp;Ai-Ping Lv ,&nbsp;Zi-Yu Zhao ,&nbsp;Wen-Dong Xian ,&nbsp;Zheng-Han Lian ,&nbsp;Yu-Ting OuYang ,&nbsp;Hong Ming ,&nbsp;Sha Tan ,&nbsp;Jian-Yu Jiao ,&nbsp;En-Min Zhou ,&nbsp;Lan Liu ,&nbsp;Wen-Jun Li","doi":"10.1016/j.syapm.2022.126361","DOIUrl":"10.1016/j.syapm.2022.126361","url":null,"abstract":"<div><p>Biological denitrification is a significant process in nitrogen biogeochemical cycle of terrestrial geothermal environments, and <em>Thermus</em> species have been shown to be crucial heterotrophic denitrifier in hydrothermal system. Five Gram-stain negative, aerobic and rod-shaped thermophilic bacterial strains were isolated from hot spring sediments in Tibet, China. Phylogenetic analysis based on 16S rRNA gene and whole genome sequences indicated that these isolates should be assigned to the genus <em>Thermus</em> and were most closely related to <em>Thermus caldifontis</em> YIM 73026^T, and <em>Thermus brockianus</em> YS38^T. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the five strains and the type strains of the genus <em>Thermus</em> were lower than the threshold values (95% and 70%, respectively) recommended for bacterial species, which clearly distinguished the five isolates from other species of the genus <em>Thermus</em> and indicated that they represent independent species. Colonies are circular, convex, non-transparent. Cell growth occurred at 37–80 °C (optimum, 60–65 °C), pH 6.0–8.0 (optimum, pH 7.0) and with 0–2.0% (w/v) NaCl (optimum, 0–0.5%). Denitrification genes (<em>narG</em>, <em>nirK</em>, <em>nirS</em>, and <em>norB</em> genes) detected in their genomes indicated their potential function in nitrogen metabolism. The obtained results combined with those of morphological, physiological, and chemotaxonomic characteristics, including the menaquinones, polar lipids, and cellular fatty acids showed that the isolates are proposed as representing five novel species of the genus <em>Thermus</em>, which are proposed as <em>Thermus hydrothermalis</em> sp. nov. SYSU G00291^T, <em>Thermus neutrinimicus</em> sp. nov. SYSU G00388^T, <em>Thermus thalpophilus</em> sp. nov. SYSU G00506^T, <em>Thermus albus</em> sp. nov. SYSU G00608^T, <em>Thermus altitudinis</em> sp. nov. SYSU G00630^T.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33494865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based taxonomic classification of the closest-to-Comamonadaceae group supports a new family Sphaerotilaceae fam. nov. and taxonomic revisions","authors":"Yang Liu,&nbsp;Juan Du,&nbsp;Tao Pei,&nbsp;Hanqin Du,&nbsp;Guang-Da Feng,&nbsp;Honghui Zhu","doi":"10.1016/j.syapm.2022.126352","DOIUrl":"10.1016/j.syapm.2022.126352","url":null,"abstract":"<div><p>Many genera closest to the family <span><em>Comamonadaceae</em></span><span> have not been classified into any family; moreover, some of them are not monophyletic groups beyond the genus level. To resolve the taxonomic uncertainty of the closest-to-</span><em>Comamonadaceae</em><span><span> (CTC) group, we performed 16S rRNA gene- and genome-based phylogenetic analyses combined with genome relatedness indices and phenotypic traits comparison. Phylogenies based on the 16S </span>rRNA gene<span> and genome sequences demonstrated that the CTC group formed a coherent and robust monophyletic lineage and was sister to the family </span></span><em>Comamonadaceae</em>, thereby proposing the CTC group as a novel family, <em>Sphaerotilaceae</em><span> fam. nov. The resolved genus- and species-level taxonomic relationships of this new family were then validated by the phylogenomic reconstruction and comparisons of genome relatedness indices including digital DNA-DNA hybridization and average nucleotide identity (ANI) as well as comprehensive phenotypic analysis for type strains. Finally, we reclassified all misidentified genera and species, resulting in 19 new combinations, and proposed </span><em>Sphaerotilaceae</em><span>-specific thresholds of ANI and average amino acid identity for genus delineation. Collectively, this study has established a sound taxonomic framework of the novel family </span><em>Sphaerotilaceae</em> and will help guide future taxonomic efforts and prevent the propagation of taxonomic errors.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40351313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A taxonomic note on the genus Prevotella: Description of four novel genera and emended description of the genera Hallella and Xylanibacter","authors":"Thomas C.A. Hitch ,&nbsp;Kevin Bisdorf ,&nbsp;Afrizal Afrizal ,&nbsp;Thomas Riedel ,&nbsp;Jörg Overmann ,&nbsp;Till Strowig ,&nbsp;Thomas Clavel","doi":"10.1016/j.syapm.2022.126354","DOIUrl":"10.1016/j.syapm.2022.126354","url":null,"abstract":"<div><p>The genus <em>Prevotella</em> comprises 55 species with validly published, and correct, names (at June 2021) that are phenotypically, ecologically and functionally diverse. This study used a range of comparative genome approaches (marker gene-based genome phylogeny, core genome phylogeny, average amino acid identity, percentage of conserved proteins and clade-specific marker genes) to identify large differences between the 53 species for which genomes are available, as well as two effectively published yet not validly named species and four novel species. These differences were consistent between the various analysis methods and justify the separation of <em>Prevotella</em> into multiple genera. While the distribution across 19 ecosystem types was unique for each species and inconsistent within clades, the functional repertoire based on the presence/absence of both PFAMs and CAZy families revealed distinct clustering based on the proposed genera. Based on the integration of all results, we propose the reclassification of species previously assigned to the genus <em>Prevotella</em> into seven genera, including four novel genera for which the names <em>Segatella</em>, <em>Hoylesella</em>, <em>Leyella</em> and <em>Palleniella</em> are proposed. In addition to the reclassification of <em>Prevotella</em>, this work describes four novel species, <em>Hallella faecis</em>, <em>Xylanibacter rodentium</em>, <em>Xylanibacter muris</em>, and <em>Palleniella intestinalis</em>.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0723202022000613/pdfft?md5=923fe0944099b7b5e2eb9b989f419c28&pid=1-s2.0-S0723202022000613-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40354241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bartonella choladocola sp. nov. and Bartonella apihabitans sp. nov., two novel species isolated from honey bee gut","authors":"Yuwen Liu,&nbsp;Jieteng Chen,&nbsp;Haoyu Lang,&nbsp;Hao Zheng","doi":"10.1016/j.syapm.2022.126372","DOIUrl":"10.1016/j.syapm.2022.126372","url":null,"abstract":"<div><p><span><em>Bartonella</em></span><span> is one of the noncore bacterial genera in the honey bee (</span><span><em>Apis mellifera</em></span>) gut. So far, only one species, <em>Bartonella apis</em>, has been described from the honey bee gut. Previous analyses based on the genomic information of isolates and metagenome-assembled genomes suggested the existence of multiple <em>Bartonella</em> species in the bee guts. Here, 10 strains were isolated and characterized from the gut of <em>A. mellifera</em> from Jilin Province, China. New isolates shared ＞95% 16S rRNA gene sequence similarity with other species of the genus <em>Bartonella</em><span>. Phylogenetic analysis revealed that new isolates clustered with other type strains of </span><em>Bartonella,</em> and the bee gut <em>Bartonella</em> could be classified into three clades. The <em>in silico</em><span><span> DDH and average nucleotide identity values between strains of different clusters from the honey bee gut are 29.1–32.5% and 87.6–89.3%, all below the recommended 70.0% and 95% cutoff points. Cells are Gram-staining-negative rods and can grow on the surface of Brain Heart Infusion </span>agar plates supplemented with defibrinated sheep blood in an aerobic environment with 5% CO</span>₂ at 35–37 °C. Strains from different species varied in both phenotypic and chemotaxonomic characterizations. Comparative genomic analysis indicated that <em>B. choladocola</em><span> had unique sets of genes encoding invasin, representing the potential for this species to both live as a gut symbiont and also as an erythrocytic pathogen. Thus, we propose two novel species </span><em>Bartonella choladocola</em> sp. nov. whose type strain is W8125^T(=JCM 35030^T = ACCC 62057^T), and <em>Bartonella apihabitans</em> sp. nov. whose type strain is W8097^T(=JCM 35029^T = ACCC 62056^T).</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40657069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic Bosea spartocytisi sp. nov. Coexists with rhizobia in root nodules of Spartocytisus supranubius growing in soils of Teide National Park (Canary Islands)","authors":"Laura Pulido-Suárez ,&nbsp;José David Flores-Félix ,&nbsp;Noemí Socas-Pérez ,&nbsp;José M. Igual ,&nbsp;Encarna Velázquez ,&nbsp;Álvaro Péix ,&nbsp;Milagros León-Barrios","doi":"10.1016/j.syapm.2022.126374","DOIUrl":"10.1016/j.syapm.2022.126374","url":null,"abstract":"<div><p>Two rod-shaped Gram negative strains, SSUT16^T and SSUT22, were isolated from root nodules of <em>Spartocytisus supranubius</em> in soils of the Teide National Park (Tenerife, Spain). The 16S rRNA gene sequences of these two novel strains classified them within genus <em>Bosea</em> with similarity values ranging from 97.65 % to 99.54 % with respect to the other species of this genus. The MLSA analysis from a concatenation of the two housekeeping- genes, <em>recA</em> and <em>gyrB</em>, showed that <em>Bosea thiooxidans</em> LMG 26210^T and <em>B. robiniae</em> LMG 26381^T are the two closest relative species with which they share similarity sequences values of 94.42 % and 94.27 %, respectively. The genome sequence analysis of strain SSUT16^T showed average nucleotide identity percentages (ANIb) and digital DNA-DNA hybridization (dDDH) below 84 % and 33 %, respectively, with the type strains of all sequenced species of genus <em>Bosea</em>. These values are much lower than the currently accepted cut-off values for these two parameters to delineate bacterial species, confirming that the novel strains constitute a novel <em>Bosea</em> species. In addition, they are also distinguished from the other closest species in their fatty acid composition and in other phenotypic characteristics. Genome sequence analysis showed the absence of the common nodulation and nitrogen fixation genes in the novel strains. Therefore, based on the results of phylogenetic, genomic, chemotaxonomic and phenotypic characterization, we propose a new species named <em>Bosea spartocytisi</em> sp. nov., with type strain SSUT16^T (=LMG 32510^T = CECT 30526^T = HAMBI 3759^T).</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0723202022000819/pdfft?md5=412a538c68462dd1bbcb55cf9498b415&pid=1-s2.0-S0723202022000819-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40467473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Dissulfurispira thermophila gen. nov., sp. nov., a thermophilic chemolithoautotroph growing by sulfur disproportionation, and proposal of novel taxa in the phylum Nitrospirota to reclassify the genus Thermodesulfovibrio” [Syst. Appl. Microbiol. 44 (2021) 126184]","authors":"Kazuhiro Umezawa,&nbsp;Hisaya Kojima,&nbsp;Yukako Kato,&nbsp;Manabu Fukui","doi":"10.1016/j.syapm.2022.126323","DOIUrl":"10.1016/j.syapm.2022.126323","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0723202022000303/pdfft?md5=02ec00646154ff62c33fca1e266aa3d2&pid=1-s2.0-S0723202022000303-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44776066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natranaeroarchaeum sulfidigenes gen. nov., sp. nov., carbohydrate-utilizing sulfur-respiring haloarchaeon from hypersaline soda lakes, a member of a new family Natronoarchaeaceae fam. nov. in the order Halobacteriales","authors":"Dimitry Y. Sorokin ,&nbsp;Michail Yakimov ,&nbsp;Enzo Messina ,&nbsp;Alexander Y. Merkel ,&nbsp;Michel Koenen ,&nbsp;Nicole J. Bale ,&nbsp;Jaap S. Sinninghe Damsté","doi":"10.1016/j.syapm.2022.126356","DOIUrl":"10.1016/j.syapm.2022.126356","url":null,"abstract":"<div><p>A pure culture of alkaliphilic haloarchaeon strain AArc-S^T capable of anaerobic growth by carbohydrate-dependent sulfur respiration was obtained from hypersaline lakes in southwestern Siberia. According to phylogenetic analysis, AArc-S^T formed a new genus level branch most related to the genus <em>Natronoarchaeum</em> in the order <em>Halobacteriales</em>. The strain is facultatively anaerobic with strictly respiratory metabolism growing either by anaerobic respiration with elemental sulfur and thiosulfate as the electron acceptors or by aerobic respiration at microoxic conditions. Thiosulfate is reduced partially to sulfide and sulfite. It is a first sulfur-reducing alkaliphilic haloarchaeon utilizing sugars, starch and glycerol as substrates for anaerobic growth. It is extremely halophilic (optimum at 3.5 M total Na⁺) and obligately alkaliphilic (optimum at pH 9.5). The dominant polar lipids include PG and PGP-Me with the archaeol (C₂₀-C₂₀) or extended archaeol (C₂₀-C₂₅) cores. The dominant respiratory lipoquinone is MK-8:8. On the basis of unique physiological properties and results of phylogenetic analysis, the soda lake isolate is suggested to be classified into a novel genus and species <em>Natranaeroarchaeum sulfidigenes</em> gen. nov., sp. nov. (=JCM 34033^T = UNIQEM U1000^T). Furthermore, on the bases of phylogenomic reconstruction, a new family <em>Natronoarchaeaceae</em> fam. nov. is proposed within the order <em>Halobacteriales</em> incorporating <em>Natranaeroarchaeum</em> and three related genera: <em>Natronoarchaeum</em>, <em>Salinarchaeum</em> and <em>Halostella</em>.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0723202022000637/pdfft?md5=98a965f95613b1fd801f0d883cf0a440&pid=1-s2.0-S0723202022000637-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40360292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The divergent genome of Scorpion Group 1 (SG1) intracellular bacterium from the venom glands of Vaejovis smithi (Scorpiones: Vaejovidae)","authors":"Tonalli García-Santibañez ,&nbsp;Mónica Rosenblueth ,&nbsp;Luis M. Bolaños ,&nbsp;Julio Martínez-Romero ,&nbsp;Esperanza Martínez-Romero","doi":"10.1016/j.syapm.2022.126358","DOIUrl":"10.1016/j.syapm.2022.126358","url":null,"abstract":"<div><p>Scorpions were among the first animals on land around 430 million years ago. Like many arachnids, scorpions have evolved complex venoms used to paralyze their prey and for self-defense. Here we sequenced and analyzed the metagenomic DNA from venom glands from <em>Vaejovis smithi</em> scorpions. A metagenome-assembled genome (MAG) of 624,025 bp was obtained corresponding to the previously reported Scorpion Group 1 (SG1). The SG1 genome from venom glands had a low GC content (25.8%) characteristic of reduced genomes, many hypothetical genes and genes from the reported minimal set of bacterial genes. Phylogenomic reconstructions placed the uncultured SG1 distant from other reported bacteria constituting a taxonomic novelty. By PCR we detected SG1 in all tested venom glands from 30 independent individuals. Microscopically, we observed SG1 inside epithelial cells from the venom glands using FISH and its presence in scorpion embryos suggested that SG1 is transferred from mother to offspring.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40382746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}