Systematic and applied microbiology最新文献

{"title":"Corrigendum to “Sphingomonas pokkalii sp. nov., a novel plant associated rhizobacterium isolated from a saline tolerant pokkali rice and its draft genome analysis” [Syst. Appl. Microbiol. 42(3) (2019) 334–342]","authors":"Rahul R. Menon , Sunitha Kumari , Pravin Kumar , Ashish Verma , Srinivasan Krishnamurthi , N. Rameshkumar","doi":"10.1016/j.syapm.2023.126450","DOIUrl":"10.1016/j.syapm.2023.126450","url":null,"abstract":"","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 5","pages":"Article 126450"},"PeriodicalIF":3.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9941224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brytella acorum gen. nov., sp. nov., a novel acetic acid bacterium from sour beverages","authors":"Atena Sadat Sombolestani ,&nbsp;Dries Bongaerts ,&nbsp;Eliza Depoorter ,&nbsp;Ilse Cleenwerck ,&nbsp;Anneleen D. Wieme ,&nbsp;Scott J. Britton ,&nbsp;Stefan Weckx ,&nbsp;Luc De Vuyst ,&nbsp;Peter Vandamme","doi":"10.1016/j.syapm.2023.126440","DOIUrl":"10.1016/j.syapm.2023.126440","url":null,"abstract":"<div><p>Polyphasic taxonomic and comparative genomic analyses revealed that a series of lambic beer isolates including strain LMG 32668^T<span><span> and the kombucha isolate LMG 32879 represent a novel species among the </span>acetic acid bacteria, with </span><em>Acidomonas methanolica</em><span> as the nearest phylogenomic neighbor with a valid name. Overall genomic relatedness indices and phylogenomic and physiological analyses revealed that this novel species was best classified in a novel genus for which we propose the name </span><em>Brytella acorum</em> gen. nov., sp. nov., with LMG 32668^T (=CECT 30723^T) as the type strain. The <em>B. acorum</em><span><span> genomes encode a complete but modified tricarboxylic acid cycle<span>, and complete pentose phosphate, </span></span>pyruvate<span> oxidation<span><span><span> and gluconeogenesis<span> pathways. The absence of 6-phosphofructokinase which rendered the glycolysis pathway non-functional, and an energy metabolism that included both aerobic respiration and oxidative fermentation are typical metabolic characteristics of acetic acid bacteria. Neither genome encodes nitrogen fixation or </span></span>nitrate reduction genes, but both genomes encode genes for the </span>biosynthesis<span> of a broad range of amino acids<span>. Antibiotic resistance<span> genes or virulence factors are absent.</span></span></span></span></span></span></p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 5","pages":"Article 126440"},"PeriodicalIF":3.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10143785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two novel symbiovars of Bradyrhizobium yuanmingense, americaense and caribense, the symbiovar tropici of Bradyrhizobium pachyrhizi and the symbiovar cajani of Bradyrhizobium cajani are microsymbionts of the legume Cajanus cajan in Dominican Republic","authors":"José David Flores-Félix ,&nbsp;Fernando Sánchez-Juanes ,&nbsp;Juan Araujo ,&nbsp;César Antonio Díaz-Alcántara ,&nbsp;Encarna Velázquez ,&nbsp;Fernando González-Andrés","doi":"10.1016/j.syapm.2023.126454","DOIUrl":"10.1016/j.syapm.2023.126454","url":null,"abstract":"<div><p><span><em>Cajanus</em><em> cajan</em></span> L. (guandul) is commonly cultivated in Dominican Republic where this legume is a subsistence crop<em>.</em> Here we identified through MALDI-TOF MS several rhizobial strains nodulating <em>C. cajan</em> in two Dominican locations as <span><em>Bradyrhizobium</em><em> yuanmingense</em></span><span>. The phylogenetic analysis of </span><em>recA</em> and <em>glnII</em><span> housekeeping genes showed that these strains belong to a wide cluster together with the type strain of </span><em>B. yuanmingense</em> and other <em>C. cajan</em><span> nodulating strains previously isolated in Dominican Republic. The comparison of genomes from strains representative of different lineages within this cluster support the existence of several genospecies within </span><em>B. yuanmingense</em><span>, which is the major microsymbiont of </span><em>C. cajan</em> in Dominican Republic where it is also nodulated by <em>Bradyrhizobium cajani</em> and <em>Bradyrhizobium pachyrhizi</em>. The analysis of the symbiotic <em>nodC</em> gene showed that the <em>C. cajan</em> nodulating strains from the <em>B. yuanmingense</em> complex belong to two clusters with less than 90% similarity between them. The strains from these two clusters showed <em>nodC</em> gene similarity values lower than 90% with respect to the remaining <em>Bradyrhizobium</em> symbiovars and then they correspond to two new symbiovars for which we propose the names americaense and caribense. The results of the <em>nodC</em> gene analysis also showed that <em>C. cajan</em> is nodulated by the symbiovar tropici, which has been found by first time in this work within the species <em>Bradyrhizobium pachyrhizi</em>. These results confirmed the high promiscuity degree of <em>C. cajan</em>, which is also nodulated by the symbiovar cajani of <em>Bradyrhizobium cajani</em> in Dominican Republic.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 5","pages":"Article 126454"},"PeriodicalIF":3.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10230913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the rumen Prevotella collection: The description of Prevotella communis, sp. nov. of ovine origin","authors":"Eva Grabner ,&nbsp;Eva Stare ,&nbsp;Lijana Fanedl ,&nbsp;Maša Zorec ,&nbsp;Dakota S. Jones ,&nbsp;Christopher D. Johnston ,&nbsp;Gorazd Avguštin ,&nbsp;Tomaž Accetto","doi":"10.1016/j.syapm.2023.126437","DOIUrl":"10.1016/j.syapm.2023.126437","url":null,"abstract":"<div><p>27 strains representing eight new <span><em>Prevotella</em></span><span> species were isolated from rumen<span> of a single sheep in eight weeks interval. One of the putative species encompassing the highest number of isolated strains which also exhibited some genetic variability<span> in preliminary data, was then selected for description of a novel species. We examined six strains in genomic and phenotypic detail, two of which may actually be the same strain isolated nearly three weeks apart. Other strains formed clearly diverged intraspecies lineages as evidenced by core genome phylogeny and phenotypic differences. Strains of the proposed new </span></span></span><em>Prevotella</em> species are strictly saccharolytic as is usual for rumen <em>Prevotella</em><span><span>, and use plant cell-wall xylans and pectins for growth. However, the range of cell-wall </span>polysaccharides<span> utilised for growth is rather limited compared to rumen generalists such as </span></span><em>Prevotella bryantii</em> or <span><em>Prevotella ruminicola</em></span> and this extends also to the inability to utilise starch, which is unexpected for the members of the genus <em>Prevotella</em>. Based on the data obtained, we propose <em>Prevotella communis</em> sp. nov. to accommodate strain E1-9^T as well as other strains with the similar properties<em>.</em><span> The proposed species is widespread: two other strains were previously isolated from sheep in Japan and is also common in metagenomic<span> data of cattle and sheep rumen samples from Scotland and New Zealand. It was also found in a collection of metagenome-assembled genomes originating from cattle in Scotland. Thus, it is a ubiquitous bacterium of domesticated ruminants specialising in degradation of a somewhat restricted set of plant cell wall components.</span></span></p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 4","pages":"Article 126437"},"PeriodicalIF":3.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10158346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial community on industrial salty bovine hides: From the slaughterhouse to the salting","authors":"Francisco Nadal-Molero ,&nbsp;Alicia Campos-Lopez ,&nbsp;Juan Tur-Moya ,&nbsp;Ana-Belen Martin-Cuadrado","doi":"10.1016/j.syapm.2023.126421","DOIUrl":"10.1016/j.syapm.2023.126421","url":null,"abstract":"<div><p>The leather-making industry is an age-old industry and desiccation with salt has been one of the most used methodologies for obtaining valuable skins. However, halophiles may proliferate and affect the integrity of the hide-collagen structure, as well as leading to undesirable red colorations or less-frequent purple stains. To understand the basis of these industrial hide contaminations, the microbial community from raw hide samples, salt-cured samples and four different industrial salts, was analyzed by 16S rRNA gene metabarcoding together with standard cultivation methods.</p><p>Comparison of raw hides and correctly cured hides revealed a core microbiome that was absent from contaminated hides. In addition, archaea were missing from well-cured hides, whereas <em>Psychrobacter</em> and <em>Acinetobacter</em> were highly represented (23 % and 17.4 %, respectively). In damaged hides, only a few operational taxonomic units (OTUs), from among the hundreds detected, were able to proliferate and, remarkably, a single <em>Halomonas</em> OTU represented 57.66 % of the reads. Halobacteria, mainly <em>Halovenus, Halorubrum</em> and <em>Halovivax,</em> increased by up to 36.24–39.5 % in the red- and purple-affected hides. The major contaminants were isolated and hide infections, together with collagenase activity, were evaluated. The results showed that hides enriched with the non-pigmented isolate <em>Halomonas utahensis</em> COIN160 damaged the collagen fibers similarly to <em>Halorubrum</em>, and together they were considered to be one of the major causes. Putative degrading inhibitors were also identified from among the <em>Alkalibacillus</em> isolates.</p><p>It was concluded that hide contaminations were driven by clonal outbreaks of a few specific microbes, which may have been non-pigmented collagen degraders. <em>Acinetobacter</em> and <em>Alkalibacillus</em>, members of the core microbiome of raw and well-cured salted hides, are suggested as hide contaminant inhibitors that need further analysis.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 4","pages":"Article 126421"},"PeriodicalIF":3.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9850658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, classification, and functional characterization of novel sponge-associated acidimicrobiial species","authors":"Viet Hung Nguyen ,&nbsp;Bernd Wemheuer ,&nbsp;Weizhi Song ,&nbsp;Holly Bennett ,&nbsp;Nicole Webster ,&nbsp;Torsten Thomas","doi":"10.1016/j.syapm.2023.126426","DOIUrl":"10.1016/j.syapm.2023.126426","url":null,"abstract":"<div><p><span>Sponges are known to harbour an exceptional diversity of uncultured microorganisms, including members of the phylum </span><em>Actinobacteriota</em>. While members of the actinobacteriotal class <em>Actinomycetia</em><span> have been studied intensively due to their potential for secondary metabolite production, the sister class of </span><em>Acidimicrobiia</em> is often more abundant in sponges. However, the taxonomy, functions, and ecological roles of sponge-associated <em>Acidimicrobiia</em> are largely unknown. Here, we reconstructed and characterized 22 metagenome-assembled genomes (MAGs) of <em>Acidimicrobiia</em> from three sponge species. These MAGs represented six novel species, belonging to five genera, four families, and two orders, which are all uncharacterized (except the order <em>Acidimicrobiales</em><span>) and for which we propose nomenclature. These six uncultured species have either only been found in sponges and/or corals and have varying degrees of specificity to their host species. Functional gene profiling indicated that these six species shared a similar potential to non-symbiotic </span><em>Acidimicrobiia</em><span><span> with respect to amino acid </span>biosynthesis<span> and utilization of sulfur compounds. However, sponge-associated </span></span><em>Acidimicrobiia</em><span><span> differed from their non-symbiotic counterparts by relying predominantly on organic rather than inorganic sources of energy, and their predicted capacity to synthesise bioactive compounds or their precursors implicated in host defence. Additionally, the species possess the </span>genetic capacity to degrade aromatic compounds that are frequently found in sponges. The novel </span><em>Acidimicrobiia</em><span> may also potentially mediate host development by modulating Hedgehog signalling and by the production of serotonin, which can affect host body contractions and digestion. These results highlight unique genomic and metabolic features of six new acidimicrobiial species that potentially support a sponge-associated lifestyle.</span></p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 4","pages":"Article 126426"},"PeriodicalIF":3.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9782668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fontisphaera persica gen. nov., sp. nov., a thermophilic hydrolytic bacterium from a hot spring of Baikal lake region, and proposal of Fontisphaeraceae fam. nov., and Limisphaeraceae fam. nov. within the Limisphaerales ord. nov. (Verrucomicrobiota)","authors":"Olga A. Podosokorskaya ,&nbsp;Alexander G. Elcheninov ,&nbsp;Andrei A. Novikov ,&nbsp;Alexander Y. Merkel ,&nbsp;Ilya V. Kublanov","doi":"10.1016/j.syapm.2023.126438","DOIUrl":"10.1016/j.syapm.2023.126438","url":null,"abstract":"<div><p><span>A novel facultatively anaerobic moderately thermophilic bacterium, strain B-154 </span>^T<span>, was isolated from a terrestrial hot spring in the Baikal lake region (Russian Federation). Gram-negative, motile, spherical cells were present singly, in pairs, or aggregates, and reproduced by binary fission. The strain grew at 30–57 °C and within a pH range of 5.1–8.4 with the optimum at 50 °C and pH 6.8–7.1. Strain B-154 </span>^T<span><span><span><span> was a chemoorganoheterotroph, growing on mono-, di- and polysaccharides (xylan, starch, </span>galactan, </span>galactomannan<span><span>, glucomannan<span>, xyloglucan, </span></span>pullulan<span>, arabinan, lichenan, beta-glucan, pachyman, </span></span></span>locust bean gum, xanthan gum). It did not require sodium chloride or yeast extract for growth. Major cellular fatty acids were anteiso-C</span>_15:0, <em>iso</em>-C_16:0 and <em>iso</em>-C_14:0<span>. The respiratory quinone was MK-7. The complete genome of strain B-154 </span>^T<span> was 4.73 Mbp in size; its G + C content was 61%. According to the phylogenomic analysis strain B-154 </span>^T<span> forms a separate family-level phylogenetic<span> lineage. Moreover, together with </span></span><em>Limisphaera ngatamarikiensis</em> and <em>“Pedosphaera parvula”</em> this strain forms a separate order-level phylogenetic lineage within <em>Verrucomicrobiae</em> class. Hence, we propose a novel order, <em>Limisphaerales</em> ord. nov., with two families <em>Limisphaeraceae</em> fam. nov. and <em>Fontisphaeraceae</em> fam. nov., and a novel genus and species <em>Fontisphaera persica</em> gen. nov., sp. nov. with type strain B-154 ^T. Ecogenomic analysis showed that representatives of the <em>Limisphaerales</em> are widespread in various environments. Although some of them were detected in hot springs the majority of <em>Limisphaerales</em><span> (54% of the studied metagenome-assembled genomes) were found in marine habitats. This study allowed a better understanding of physiology and ecology of </span><em>Verrucomicrobiota</em><span> – a rather understudied bacterial phylum.</span></p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 4","pages":"Article 126438"},"PeriodicalIF":3.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9851738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two novel species isolated from wheat rhizospheres in Serbia: Pseudomonas serbica sp. nov. and Pseudomonas serboccidentalis sp. nov.","authors":"Irena Todorović ,&nbsp;Danis Abrouk ,&nbsp;Martina Kyselková ,&nbsp;Céline Lavire ,&nbsp;Marjolaine Rey ,&nbsp;Vera Raičević ,&nbsp;Jelena Jovičić-Petrović ,&nbsp;Yvan Moënne-Loccoz ,&nbsp;Daniel Muller","doi":"10.1016/j.syapm.2023.126425","DOIUrl":"10.1016/j.syapm.2023.126425","url":null,"abstract":"<div><p><em>Pseudomonas</em> strains IT-194P, IT-215P, IT-P366^T and IT-P374^T were isolated from the rhizospheres of wheat grown in soils sampled from different fields (some of them known to be disease-suppressive) located near Mionica, Serbia. Phylogenetic analysis of the 16S rRNA genes and of whole genome sequences showed that these strains belong to two potentially new species, one containing strains IT-P366^T and IT-194P and clustering (whole genome analysis) next to <em>P. umsongensis</em> DSM16611^T, and another species containing strains IT-P374^T and IT-215P and clustering next to <em>P. koreensis</em> LMG21318^T. Genome analysis confirmed the proposition of novel species, as ANI was below the threshold of 95% and dDDH below 70% for strains IT-P366^T (compared with <em>P. umsongensis</em> DSM16611^T) and IT-P374^T (compared with <em>P. koreensis</em> LMG21318^T). Unlike <em>P. umsongensis</em> DSM16611^T, strains of <em>P. serbica</em> can grow on D-mannitol, but not on pectin, D-galacturonic acid, L-galactonic acid lactone and α-hydroxybutyric acid. In contrary to <em>P. koreensis</em> LMG21318^T, strains of <em>P. serboccidentalis</em> can use sucrose, inosine and α-ketoglutaric acid (but not L-histidine) as carbon sources. Altogether, these results indicate the existence of two novel species for which we propose the names <em>Pseudomonas serbica</em> sp. nov., with the type strain IT-P366^T (=CFBP 9060 ^T = LMG 32732 ^T = EML 1791 ^T) and <em>Pseudomonas serboccidentalis</em> sp. nov., with the type strain IT-P374^T (=CFBP 9061 ^T = LMG 32734 ^T = EML 1792 ^T). Strains from this study presented a set of phytobeneficial functions modulating plant hormonal balance, plant nutrition and plant protection, suggesting a potential as Plant Growth-Promoting Rhizobacteria (PGPR).</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 4","pages":"Article 126425"},"PeriodicalIF":3.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9848542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of Dellaglioa carnosa sp. nov., a novel species isolated from high-oxygen modified-atmosphere packaged meat","authors":"Victoria Werum,&nbsp;Matthias Ehrmann","doi":"10.1016/j.syapm.2023.126423","DOIUrl":"10.1016/j.syapm.2023.126423","url":null,"abstract":"<div><p><span>The study provides a taxonomic characterization of three bacterial strains isolated from high-oxygen modified-atmosphere packaged beef from Germany. The strains of the novel species shared identical 16S rRNA gene sequence to the closely related type strain of </span><em>Dellaglioa algida</em>. However, the <em>in-silico</em> DNA-DNA hybridization (DDH) values indicate that they belong to a different genomic species. The <em>in silico</em> DDH estimate value between TMW 2.2523^T and the type strain of <em>Dellaglioa algida</em> DSM 15638^T was only 63.2 %. The whole genome average nucleotide identity blast (ANIb) value of 95.1 % between TMW 2.2523^T and the closely related type strain of <em>D. algida</em><span><span> was within the recommended threshold value of 95–96 % for bacterial species delineation. Additionally, the phylogenomic analyses based on multi locus </span>sequence alignment (MLSA) showed that strain TMW 2.2523</span>^T<span> and additional strains TMW 2.2444 and TMW 2.2533 formed a monophyletic group separate from </span><em>D. algida</em><span> strains. Furthermore, tyrosine decarboxylase activity could be attributed to strains of the new proposed species. The results of this polyphasic approach support the affiliation of these strains to a novel species within the genus </span><em>Dellaglioa</em> for which we propose the name <em>Dellaglioa carnosa</em> sp. nov. The designated respective type strain is TMW 2.2523^T (DSM 114968^T = LMG 32819^T).</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 4","pages":"Article 126423"},"PeriodicalIF":3.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9792893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity, symbiotic efficiency, stress tolerance, and plant growth promotion traits of rhizobia nodulating Vachellia tortilis subsp. raddiana growing in dryland soils in southern Morocco","authors":"Mohamed Hnini,&nbsp;Imane El Attar,&nbsp;Kaoutar Taha,&nbsp;Jamal Aurag","doi":"10.1016/j.syapm.2023.126434","DOIUrl":"10.1016/j.syapm.2023.126434","url":null,"abstract":"<div><p><span>In the present study, we analyzed the genetic diversity<span>, phylogenetic relationships<span>, stress tolerance, phytobeneficial traits, and symbiotic characteristics of rhizobial strains isolated from root nodules of </span></span></span><span><em>Vachellia</em><em> tortilis</em></span> subsp<em>. raddiana</em> grown in soils collected in the extreme Southwest of the Anti-Atlas Mountains in Morocco. Subsequent to Rep-PCR fingerprinting, <em>16S rDNA</em> gene sequencing of 15 representative strains showed that all of them belong to the genus <span><em>Ensifer</em></span><span>. Phylogenetic analysis and concatenation of the housekeeping genes </span><span><em>gyrB</em></span>, <em>rpoB</em>, <em>recA</em>, and <em>dnaK</em> revealed that the entire collection (except strain LMR678) shared 99.08 % to 99.92% similarity with <em>Ensifer</em> sp. USDA 257 and 96.92% to 98.79% with <span><em>Sinorhizobium</em></span> BJ1. Phylogenetic analysis of <em>nodC</em> and <em>nodA</em> sequences showed that all strains but one (LMR678) formed a phylogenetic group with the type strain “<em>E. aridi”</em> LMR001^T (similarity over 98%). Moreover, it was relevant that most strains belong to the symbiovar vachelliae. <em>In vitro</em><span> tests revealed that five strains produced IAA<span><span>, four solubilized inorganic phosphate, and one produced </span>siderophores. All strains showed tolerance to NaCl concentrations ranging from 2 to 12% and grew at up to 10% of PEG6000. A greenhouse plant inoculation test conducted during five months demonstrated that most rhizobial strains were infective and efficient. Strains LMR688, LMR692, and LMR687 exhibited high relative symbiotic efficiency values (respectively 231.6 %, 171.96 %, and 140.84 %). These strains could be considered as the most suitable candidates for inoculation of </span></span><em>V. t.</em> subsp<em>. raddiana</em><span><span>, to be used as a pioneer plant for restoring arid soils threatened with </span>desertification.</span></p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 4","pages":"Article 126434"},"PeriodicalIF":3.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9784838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}