首次单菌株富集 Electrothrix 电缆细菌,描述 E. aestuarii sp.

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Lea E. Plum-Jensen, Andreas Schramm, Ian P.G. Marshall
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引用次数: 0

摘要

电缆细菌是一种导电的丝状脱硫杆菌科细菌,在形态、功能和系统发育上都有别于该科的其他成员。电缆细菌尚未获得纯培养物,因此以前被描述为候选属,即 Candidatus Electrothrix 和 Ca.后者的代表菌株可通过单菌株沉积物富集获得。在此,我们介绍了一种改进的工作流程,首次获得了 Ca.Electrothrix,并报告了它们的元基因组组装基因组(MAGs)和形态。根据这些结果以及之前发表的这两个候选属的电缆菌的高质量 MAGs 和形态学数据,我们建议按照《根据序列数据描述的原核生物命名规范》(SeqCode)的规则,采用 Electrothrix 和 Electronema 作为属名,分别以 Electrothrix communis RBTS 和 Electronema aureum GSTS 作为属名的命名类型。此外,根据与任何已描述物种的平均核苷酸同一性(ANI)值<95%,我们提议将我们的三个单菌株富集培养物中的两个作为 Electrothrix 属的新物种,根据 SeqCode 命名为 E. aestuarii sp.nov.和 E. rattekaaiensis sp.nov.。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
First single-strain enrichments of Electrothrix cable bacteria, description of E. aestuarii sp. nov. and E. rattekaaiensis sp. nov., and proposal of a cable bacteria taxonomy following the rules of the SeqCode

Cable bacteria are electrically conductive, filamentous Desulfobulbaceae, which are morphologically, functionally, and phylogenetically distinct from the other members of this family. Cable bacteria have not been obtained in pure culture and were therefore previously described as candidate genera, Candidatus Electrothrix and Ca. Electronema; a representative of the latter is available as single-strain sediment enrichment. Here we present an improved workflow to obtain the first single-strain enrichments of Ca. Electrothrix and report their metagenome-assembled genomes (MAGs) and morphology. Based on these results and on previously published high-quality MAGs and morphological data of cable bacteria from both candidate genera, we propose to adopt the genus names Electrothrix and Electronema following the rules of the Code of Nomenclature of Prokaryotes Described from Sequence Data (SeqCode), with Electrothrix communis RBTS and Electronema aureum GSTS, respectively, as the nomenclatural types of the genera. Furthermore, based on average nucleotide identity (ANI) values < 95 % with any described species, we propose two of our three single-strain enrichment cultures as novel species of the genus Electrothrix, with the names E. aestuarii sp. nov. and E. rattekaaiensis sp. nov., according to the SeqCode.

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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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