Multiphasic investigations imply transfer of orange-/red-pigmented strains of the bean pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens to a new species as C. aurantiacum sp. nov., elevation of the poinsettia pathogen C. flaccumfaciens pv. poinsettiae to the species level as C. Poinsettiae sp. nov., and synonymy of C. albidum with C. citreum
IF 4.3 3区 材料科学Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Ebrahim Osdaghi , S. Mohsen Taghavi , Mozhde Hamidizade , Mehdi Kariminejhad , Amal Fazliarab , Habibeh Hajian Maleki , Steve Baeyen , Geraldine Taghouti , Marie-Agnes Jacques , Johan Van Vaerenbergh , Perrine Portier
{"title":"Multiphasic investigations imply transfer of orange-/red-pigmented strains of the bean pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens to a new species as C. aurantiacum sp. nov., elevation of the poinsettia pathogen C. flaccumfaciens pv. poinsettiae to the species level as C. Poinsettiae sp. nov., and synonymy of C. albidum with C. citreum","authors":"Ebrahim Osdaghi , S. Mohsen Taghavi , Mozhde Hamidizade , Mehdi Kariminejhad , Amal Fazliarab , Habibeh Hajian Maleki , Steve Baeyen , Geraldine Taghouti , Marie-Agnes Jacques , Johan Van Vaerenbergh , Perrine Portier","doi":"10.1016/j.syapm.2024.126489","DOIUrl":null,"url":null,"abstract":"<div><p><em>Curtobacterium flaccumfaciens</em> (<em>Microbacteriaceae</em>), a plant-pathogenic coryneform species includes five pathovars with valid names and a number of proposed – but unvalidated – new members. In this study, phenotypic features and DNA similarity indexes were investigated among all <em>C. flaccumfaciens</em> members. Results showed that the <em>C. flaccumfaciens</em> pv. <em>poinsettiae</em> strains causing bacterial canker of <em>Euphorbia pulcherrima</em> in the USA as well as the orange-/red-pigmented strains of <em>C. flaccumfaciens</em> pv. <em>flaccumfaciens</em> pathogenic on dry beans in Iran are too distinct from each other and from the type strain of the species to be considered members of <em>C. flaccumfaciens</em>. Hence, the latter two groups were elevated at the species level as <em>C. poinsettiae</em> sp. nov. (ATCC 9682<sup>T</sup> = CFBP 2403<sup>T</sup> = ICMP 2566<sup>T</sup> = LMG 3715<sup>T</sup> = NCPPB 854<sup>T</sup> as type strain), and <em>C. aurantiacum</em> sp. nov. (50R<sup>T</sup> = CFBP 8819<sup>T</sup> = ICMP 22071<sup>T</sup> as type strain). Within the emended species <em>C. flaccumfaciens</em> comb. nov., yellow-pigmented strains causing bacterial wilt of dry beans and those causing bacterial canker of <em>Euphorbia pulcherrima</em> in Europe were retained as <em>C. flaccumfaciens</em> pv. <em>flaccumfaciens</em> and <em>C. flaccumfaciens</em> pv<em>. poinsettiae,</em> respectively; while taxonomic position of the sugar beet pathogen <em>C. flaccumfaciens</em> pv. <em>beticola</em> ATCC BAA144<sup>PT</sup> was confirmed. The newly described onion pathogen <em>C. allii</em> was also reclassified as <em>C. flaccumfaciens</em> pv<em>. allii</em> with the pathotype strain LMG 32517<sup>PT</sup>. Furthermore, <em>C. flaccumfaciens</em> pv. <em>basellae</em> causing bacterial leaf spot of malabar spinach (<em>Basella rubra</em>) was transferred to <em>C. citreum</em> pv. <em>basellae</em> with ATCC BAA143<sup>PT</sup> as pathotype.</p></div>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202024000031","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0
Abstract
Curtobacterium flaccumfaciens (Microbacteriaceae), a plant-pathogenic coryneform species includes five pathovars with valid names and a number of proposed – but unvalidated – new members. In this study, phenotypic features and DNA similarity indexes were investigated among all C. flaccumfaciens members. Results showed that the C. flaccumfaciens pv. poinsettiae strains causing bacterial canker of Euphorbia pulcherrima in the USA as well as the orange-/red-pigmented strains of C. flaccumfaciens pv. flaccumfaciens pathogenic on dry beans in Iran are too distinct from each other and from the type strain of the species to be considered members of C. flaccumfaciens. Hence, the latter two groups were elevated at the species level as C. poinsettiae sp. nov. (ATCC 9682T = CFBP 2403T = ICMP 2566T = LMG 3715T = NCPPB 854T as type strain), and C. aurantiacum sp. nov. (50RT = CFBP 8819T = ICMP 22071T as type strain). Within the emended species C. flaccumfaciens comb. nov., yellow-pigmented strains causing bacterial wilt of dry beans and those causing bacterial canker of Euphorbia pulcherrima in Europe were retained as C. flaccumfaciens pv. flaccumfaciens and C. flaccumfaciens pv. poinsettiae, respectively; while taxonomic position of the sugar beet pathogen C. flaccumfaciens pv. beticola ATCC BAA144PT was confirmed. The newly described onion pathogen C. allii was also reclassified as C. flaccumfaciens pv. allii with the pathotype strain LMG 32517PT. Furthermore, C. flaccumfaciens pv. basellae causing bacterial leaf spot of malabar spinach (Basella rubra) was transferred to C. citreum pv. basellae with ATCC BAA143PT as pathotype.
多相调查表明,豆类病原体 Curtobacterium flaccumfaciens pv. flaccumfaciens 的橙色/红色色素菌株被列为新种 C. aurantiacum sp.nov.,一品红病原体 C. flaccumfaciens pv. poinsettiae 被列为新种 C. Poinsettiae sp.nov.,C. albidum 与 C. citreum 被列为同物异名。
Curtobacterium flaccumfaciens(微细菌科)是一种植物致病杆菌,包括五个具有有效名称的病原菌和一些拟议中但未经验证的新成员。在这项研究中,对所有 C. flaccumfaciens 成员的表型特征和 DNA 相似性指数进行了调查。结果表明,在美国引起大戟科植物细菌性腐烂病的 C. flaccumfaciens pv. poinsettiae 菌株,以及在伊朗干豆上致病的 C. flaccumfaciens pv. flaccumfaciens 的橙色/红色色素菌株,彼此之间以及与该物种的模式菌株之间的差异太大,不能被认为是 C. flaccumfaciens 的成员。新种(ATCC 9682 T = CFBP 2403 T = ICMP 2566 T = LMG 3715 T = NCPPB 854 T 为模式菌株),以及 C. aurantiacum sp.flaccumfaciens pv. flaccumfaciens 和 C. flaccumfaciens pv. poinsettiae;而甜菜病原体 C. flaccumfaciens pv. beticola ATCC BAA144PT 的分类位置得到了确认。新描述的洋葱病原体 C. allii 也被重新归类为 C. flaccumfaciens pv. allii,病原型为菌株 LMG 32517PT。此外,导致马拉巴尔菠菜(Basella rubra)细菌性叶斑病的 C. flaccumfaciens pv. basellae 被转为 C. citreum pv. basellae,病原型为 ATCC BAA143PT。