分离自斯特勒海狮的口腔生活兼性厌氧放线菌 Stomatohabitans albus gen.

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zi-Wen Yang , Wen-Li Liu , Peng Zhang , Dan-Yuan Guo , Hong-Chuan Wang , Jia-Ling Li , Pan-Deng Wang , Gui-Xin Dong , Guo-Xing Nie , Wen-Jun Li
{"title":"分离自斯特勒海狮的口腔生活兼性厌氧放线菌 Stomatohabitans albus gen.","authors":"Zi-Wen Yang ,&nbsp;Wen-Li Liu ,&nbsp;Peng Zhang ,&nbsp;Dan-Yuan Guo ,&nbsp;Hong-Chuan Wang ,&nbsp;Jia-Ling Li ,&nbsp;Pan-Deng Wang ,&nbsp;Gui-Xin Dong ,&nbsp;Guo-Xing Nie ,&nbsp;Wen-Jun Li","doi":"10.1016/j.syapm.2024.126501","DOIUrl":null,"url":null,"abstract":"<div><p>Two novel actinobacteria, designated as SYSU M7M538<sup>T</sup> and SYSU M7M531, were isolated from oral of <em>Eumetopias jubatus</em> in Zhuhai Chimelong Ocean Kingdom, China. The cells of these microorganisms stained Gram-positive and were rod shaped. These strains were facultative anaerobic, and catalase-positive. Optimal growth occurred at 37 °C and pH 7.0 over 7 days of cultivation. Both strains possessed diphosphatidylglycerol, phosphatidylglycerol and phosphocholine as the major polar lipids. The main menaquinone was MK-9(H<sub>4</sub>). The major fatty acids were C<sub>16:0</sub>, C<sub>17:1</sub> <em><sub>w</sub></em><sub>8</sub><em><sub>c</sub></em>, C<sub>17:0</sub>, C<sub>18:1</sub> <em><sub>w</sub></em><sub>9</sub><em><sub>c</sub></em> and C<sub>18:0</sub>. Analyses of genome sequences revealed that the genome size of SYSU M7M538<sup>T</sup> was 2.1 Mbp with G + C content of 52.5 %, while the genome size of SYSU M7M531 was 2.3 Mbp with G + C content of 52.7 %. The ANI and 16S rRNA gene analysis results showed that the pairwise similarities between the two strains and other recognized <em>Nitriliruptoria</em> species were less than 64.9 % and 89.0 %, respectively. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strains SYSU M7M538<sup>T</sup> and SYSU M7M531 formed a well-separated phylogenetic branch distinct from other orders of <em>Nitriliruptoria</em>. Based on the data presented here, these two strains are considered to represent a novel species of a novel genus, for which the name <em>Stomatohabitans albus</em> gen. nov., sp. nov., with the type strain SYSU M7M538<sup>T</sup> (=KCTC 59113<sup>T</sup> = GDMCC 1.4286<sup>T</sup>), are proposed. We also propose that these organisms represent a novel family named <em>Stomatohabitantaceae</em> fam. nov. of a novel order <em>Stomatohabitantales</em> ord. nov.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"47 2","pages":"Article 126501"},"PeriodicalIF":3.3000,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Stomatohabitans albus gen. nov., sp. nov., an oral living facultative anaerobic actinobacteria isolated form Steller sea lion, and proposal of Stomatohabitantaceae fam. nov. and Stomatohabitantales ord. nov\",\"authors\":\"Zi-Wen Yang ,&nbsp;Wen-Li Liu ,&nbsp;Peng Zhang ,&nbsp;Dan-Yuan Guo ,&nbsp;Hong-Chuan Wang ,&nbsp;Jia-Ling Li ,&nbsp;Pan-Deng Wang ,&nbsp;Gui-Xin Dong ,&nbsp;Guo-Xing Nie ,&nbsp;Wen-Jun Li\",\"doi\":\"10.1016/j.syapm.2024.126501\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Two novel actinobacteria, designated as SYSU M7M538<sup>T</sup> and SYSU M7M531, were isolated from oral of <em>Eumetopias jubatus</em> in Zhuhai Chimelong Ocean Kingdom, China. The cells of these microorganisms stained Gram-positive and were rod shaped. These strains were facultative anaerobic, and catalase-positive. Optimal growth occurred at 37 °C and pH 7.0 over 7 days of cultivation. Both strains possessed diphosphatidylglycerol, phosphatidylglycerol and phosphocholine as the major polar lipids. The main menaquinone was MK-9(H<sub>4</sub>). The major fatty acids were C<sub>16:0</sub>, C<sub>17:1</sub> <em><sub>w</sub></em><sub>8</sub><em><sub>c</sub></em>, C<sub>17:0</sub>, C<sub>18:1</sub> <em><sub>w</sub></em><sub>9</sub><em><sub>c</sub></em> and C<sub>18:0</sub>. Analyses of genome sequences revealed that the genome size of SYSU M7M538<sup>T</sup> was 2.1 Mbp with G + C content of 52.5 %, while the genome size of SYSU M7M531 was 2.3 Mbp with G + C content of 52.7 %. The ANI and 16S rRNA gene analysis results showed that the pairwise similarities between the two strains and other recognized <em>Nitriliruptoria</em> species were less than 64.9 % and 89.0 %, respectively. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strains SYSU M7M538<sup>T</sup> and SYSU M7M531 formed a well-separated phylogenetic branch distinct from other orders of <em>Nitriliruptoria</em>. Based on the data presented here, these two strains are considered to represent a novel species of a novel genus, for which the name <em>Stomatohabitans albus</em> gen. nov., sp. nov., with the type strain SYSU M7M538<sup>T</sup> (=KCTC 59113<sup>T</sup> = GDMCC 1.4286<sup>T</sup>), are proposed. We also propose that these organisms represent a novel family named <em>Stomatohabitantaceae</em> fam. nov. of a novel order <em>Stomatohabitantales</em> ord. nov.</p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":\"47 2\",\"pages\":\"Article 126501\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-03-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202024000158\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202024000158","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

从中国珠海长隆海洋王国的大鳞蜥口腔中分离出两种新型放线菌,分别命名为 SYSU M7M538T 和 SYSU M7M531。这些微生物的细胞呈革兰氏阳性,呈杆状。这些菌株为兼性厌氧菌,过氧化氢酶阳性。在 37 °C、pH 值为 7.0 的条件下培养 7 天,可达到最佳生长状态。两种菌株的主要极性脂质为二磷脂酰甘油、磷脂酰甘油和磷脂酰胆碱。主要的甲萘醌是 MK-9(H4)。主要脂肪酸为 C16:0、C17:1 w8c、C17:0、C18:1 w9c 和 C18:0。基因组序列分析表明,SYSU M7M538T 的基因组大小为 2.1 Mbp,G + C 含量为 52.5 %,而 SYSU M7M531 的基因组大小为 2.3 Mbp,G + C 含量为 52.7 %。ANI 和 16S rRNA 基因分析结果表明,两株菌株与其他已知 Nitriliruptoria 物种的配对相似度分别小于 64.9 % 和 89.0 %。16S rRNA基因序列的系统进化分析表明,SYSU M7M538T和SYSU M7M531菌株形成了一个与其他Nitriliruptoria目截然不同的系统进化分支。根据本文提供的数据,我们认为这两株菌株代表了一个新属的新种,并将其命名为 Stomatohabitans albus gen.我们还提出这些生物代表一个新科,命名为 Stomatohabitantaceae fam.
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Stomatohabitans albus gen. nov., sp. nov., an oral living facultative anaerobic actinobacteria isolated form Steller sea lion, and proposal of Stomatohabitantaceae fam. nov. and Stomatohabitantales ord. nov

Two novel actinobacteria, designated as SYSU M7M538T and SYSU M7M531, were isolated from oral of Eumetopias jubatus in Zhuhai Chimelong Ocean Kingdom, China. The cells of these microorganisms stained Gram-positive and were rod shaped. These strains were facultative anaerobic, and catalase-positive. Optimal growth occurred at 37 °C and pH 7.0 over 7 days of cultivation. Both strains possessed diphosphatidylglycerol, phosphatidylglycerol and phosphocholine as the major polar lipids. The main menaquinone was MK-9(H4). The major fatty acids were C16:0, C17:1 w8c, C17:0, C18:1 w9c and C18:0. Analyses of genome sequences revealed that the genome size of SYSU M7M538T was 2.1 Mbp with G + C content of 52.5 %, while the genome size of SYSU M7M531 was 2.3 Mbp with G + C content of 52.7 %. The ANI and 16S rRNA gene analysis results showed that the pairwise similarities between the two strains and other recognized Nitriliruptoria species were less than 64.9 % and 89.0 %, respectively. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strains SYSU M7M538T and SYSU M7M531 formed a well-separated phylogenetic branch distinct from other orders of Nitriliruptoria. Based on the data presented here, these two strains are considered to represent a novel species of a novel genus, for which the name Stomatohabitans albus gen. nov., sp. nov., with the type strain SYSU M7M538T (=KCTC 59113T = GDMCC 1.4286T), are proposed. We also propose that these organisms represent a novel family named Stomatohabitantaceae fam. nov. of a novel order Stomatohabitantales ord. nov.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信