Paraflavitalea pollutisoli sp. nov., Pollutibacter soli gen. nov. sp. nov., Polluticoccus soli gen. nov. sp. nov., and Terrimonas pollutisoli sp. nov., four new members of the family Chitinophagaceae from polluted soil

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ze-Shen Liu , Xiao-Kang Wang , Ke-Huan Wang , Mei-Ling Yang , De-Feng Li , Shuang-Jiang Liu
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引用次数: 0

Abstract

A taxonomic investigation was conducted on four bacterial strains isolated from soil contaminated with polycyclic aromatic hydrocarbons and heavy metals. Phylogenetic analysis revealed that these strains belonged to the family Chitinophagaceae. Examination of the 16S rRNA genes indicated that their sequence identities were below 97.6 % compared to any known and validly nominated bacterial species. The genomes of the four strains ranged from 4.12 to 8.76 Mb, with overall G + C molar contents varying from 41.28 % to 50.39 %. Predominant cellular fatty acids included iso-C15:0, iso-C15:1 G, and iso-C17:0 3-OH. The average nucleotide identity ranged from 66.90 % to 74.63 %, and digital DNA-DNA hybridization was 12.5–12.8 %. Based on the genomic and phenotypic features of the new strains, four novel species and two new genera were proposed within the family Chitinophagaceae. The ecological distributions were investigated by data-mining of NCBI databases, and results showed that additional strains or species of the newly proposed taxa were widely distributed in various environments, including polluted soil and waters. Functional analysis demonstrated that strains H1-2-19XT, JS81T, and JY13-12T exhibited resistance to arsenite (III) and chromate (VI). The proposed names for the four novel species are Paraflavitalea pollutisoli (type strain H1-2-19XT = JCM 36460T = CGMCC 1.61321T), Terrimonas pollutisoli (type strain H1YJ31T = JCM 36215T = CGMCC 1.61343T), Pollutibacter soli (type strain JS81T = JCM 36462T = CGMCC 1.61338T), and Polluticoccus soli (type strain JY13-12T = JCM 36463T = CGMCC 1.61341T).

来自污染土壤的壳斗科四个新成员 Paraflavitalea pollutisoli sp.
对从受多环芳烃和重金属污染的土壤中分离出的四种细菌菌株进行了分类研究。系统进化分析表明,这些菌株属于......科。对 16S rRNA 基因的研究表明,与任何已知和有效命名的细菌物种相比,它们的序列相同度低于 97.6%。四株菌株的基因组大小从 4.12 到 8.76 Mb 不等,总的 G + C 摩尔含量从 41.28 % 到 50.39 % 不等。主要的细胞脂肪酸包括-C、-C G和-C 3-OH。平均核苷酸同一性从 66.90 % 到 74.63 % 不等,数字 DNA-DNA 杂交率为 12.5-12.8%。根据新菌株的基因组和表型特征,提出了甲壳素噬菌科中的 4 个新种和 2 个新属。通过对 NCBI 数据库进行数据挖掘,对其生态分布进行了研究,结果表明新提出的类群中的新菌株或新菌种广泛分布于各种环境中,包括受污染的土壤和水域。功能分析表明,菌株 H1-2-19X、JS81 和 JY13-12 对亚砷酸盐(III)和铬酸盐(VI)具有抗性。这四个新物种的拟命名为:(模式菌株 H1-2-19X = JCM 36460 = CGMCC 1.61321)、(模式菌株 H1YJ31 = JCM 36215 = CGMCC 1.61343)、(模式菌株 JS81 = JCM 36462 = CGMCC 1.61338)和(模式菌株 JY13-12 = JCM 36463 = CGMCC 1.61341)。
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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