Olga A. Podosokorskaya , Alexander Y. Merkel , Andrei A. Novikov , Alexandra A. Klyukina , Alexander G. Elcheninov
{"title":"Pyranulibacter aquaticus gen. nov., sp. nov., the first nitrate-reducing representative of the class Ignavibacteria from an Iturup well","authors":"Olga A. Podosokorskaya , Alexander Y. Merkel , Andrei A. Novikov , Alexandra A. Klyukina , Alexander G. Elcheninov","doi":"10.1016/j.syapm.2025.126639","DOIUrl":null,"url":null,"abstract":"<div><div>A novel facultatively anaerobic moderately thermophilic bacterium, strain 4301-Me<sup>T</sup> was isolated from the well 13k on the Iturup Island (Kuril Islands, Russian Federation). Gram-stain-negative, motile rods were present singly, in pairs, rosettes, and aggregates, or formed biofilms. The strain grew optimally at 50–55 °C, pH 6.7–7.0 and 0.2–0.4 % NaCl. It did not require yeast extract for growth and utilized mono-, di- and polysaccharides (cellulose, starch, xanthan gum, locust bean gum, glucomannan, lichenan, amorphous chitin) as well as proteinaceous substrates (gelatin, peptone, beef and yeast extract). Growth under anaerobic conditions was observed in the presence and absence of external electron acceptors. Sulfur, thiosulfate, nitrate, arsenate, Fe-citrate, and ferrihydrite were reduced with acetate, or yeast extract as electron donors. The respiratory quinone was MK-7. Major cellular fatty acids were iso-C<sub>15:0</sub>, iso-C<sub>16:0</sub>, anteiso-C<sub>15:0</sub>, 3OH-C<sub>14:0</sub>, 3OH-C<sub>13:0</sub>. The size of the genome and genomic DNA G + C content of strain 4301-Me<sup>T</sup> were 3.34 Mb and 33 %, respectively. According to the 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 4301-Me<sup>T</sup> represented a distinct lineage of the family <em>Melioribacteraceae</em> within the class <em>Ignavibacteria</em>. Based on phylogenomic analysis and phenotypic features, the novel isolate was assigned to a novel genus, for which the name <em>Pyranulibacter</em> gen. nov. is proposed. Strain 4301-Me<sup>T</sup> represents its type species <em>Pyranulibacter aquaticus</em> sp. nov. (=CGMCC 1.18061<sup>T</sup> = B-3822<sup>T</sup> = BIM B-2047<sup>T</sup> = UQM 41592<sup>T</sup>).</div></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"48 5","pages":"Article 126639"},"PeriodicalIF":3.3000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S072320202500061X","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A novel facultatively anaerobic moderately thermophilic bacterium, strain 4301-MeT was isolated from the well 13k on the Iturup Island (Kuril Islands, Russian Federation). Gram-stain-negative, motile rods were present singly, in pairs, rosettes, and aggregates, or formed biofilms. The strain grew optimally at 50–55 °C, pH 6.7–7.0 and 0.2–0.4 % NaCl. It did not require yeast extract for growth and utilized mono-, di- and polysaccharides (cellulose, starch, xanthan gum, locust bean gum, glucomannan, lichenan, amorphous chitin) as well as proteinaceous substrates (gelatin, peptone, beef and yeast extract). Growth under anaerobic conditions was observed in the presence and absence of external electron acceptors. Sulfur, thiosulfate, nitrate, arsenate, Fe-citrate, and ferrihydrite were reduced with acetate, or yeast extract as electron donors. The respiratory quinone was MK-7. Major cellular fatty acids were iso-C15:0, iso-C16:0, anteiso-C15:0, 3OH-C14:0, 3OH-C13:0. The size of the genome and genomic DNA G + C content of strain 4301-MeT were 3.34 Mb and 33 %, respectively. According to the 16S rRNA gene sequence and conserved protein sequences phylogenies, strain 4301-MeT represented a distinct lineage of the family Melioribacteraceae within the class Ignavibacteria. Based on phylogenomic analysis and phenotypic features, the novel isolate was assigned to a novel genus, for which the name Pyranulibacter gen. nov. is proposed. Strain 4301-MeT represents its type species Pyranulibacter aquaticus sp. nov. (=CGMCC 1.18061T = B-3822T = BIM B-2047T = UQM 41592T).
期刊介绍:
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: