Classification of a novel Serratia species, isolated from a wound swab in North Rhine-Westphalia: Proposal of Serratia sarumanii sp. nov

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Levin Joe Klages , Olaf Kaup , Tobias Busche , Jörn Kalinowski , Christian Rückert-Reed
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引用次数: 0

Abstract

Novel, white-pigmented, Gram-negative bacterial strains (K-M0706T, K-M0228, K-M0252, K-M0260) were isolated from clinical samples. With a similarity of up to 69.7 % to Serratia nevei S15T and up to 63.8 % to Serratia marcescens ATCC 13880T, as determined by digital DNA-DNA hybridization, the strains were assigned as novel species of the genus Serratia. The species can easily be differentiated from the red colored Serratia marcescens sensu stricto by its white phenotype. Discrimination between this strain and Serratia nevei is possible due to alpha-glucosidase activity and O/129-resistance, as shown for strain K-M0706T. The major fatty acids were determined as myristate, palmitate, cis–9,10-methylenehexadecanoate, linoleate, and (all cis-9,10)-methyleneoctadecanoate. These phenotypical and genomic data support the assignment of a novel species within the genus Serratia, named S. sarumanii due to its pathogenicity and white phenotype, with strain K-M0706T as the type strain.

从北莱茵-威斯特法伦州的伤口拭子中分离出的新型沙雷氏菌的分类:提出新的沙雷氏菌(Serratia sarumanii sp.
从临床样本中分离出了新的白色色素革兰氏阴性细菌菌株(K-M0706T、K-M0228、K-M0252、K-M0260)。经数字 DNA-DNA 杂交测定,这些菌株与 Serratia nevei S15T 的相似度高达 69.7%,与 Serratia marcescens ATCC 13880T 的相似度高达 63.8%,因此被认定为 Serratia 属的新种。该菌株的白色表型很容易与红色的严格意义上的 Serratia marcescens 区分开来。如菌株 K-M0706T 所示,该菌株具有α-葡萄糖苷酶活性和对 O/129 的抗性,因此可以将其与新韦氏沙雷氏菌区分开来。主要脂肪酸被确定为肉豆蔻酸、棕榈酸、顺式-9,10-亚甲基十六烷酸、亚油酸和(所有顺式-9,10)亚甲基十八烷酸。这些表型和基因组数据支持在沙雷氏菌属中划分出一个新物种,因其致病性和白色表型而被命名为沙雷氏菌(S. sarumanii),菌株 K-M0706T 为模式菌株。
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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