Rafael Bustamante-Brito , Arturo Vera-Ponce de León , Mónica Rosenblueth , Esperanza Martínez-Romero
{"title":"胭脂鱼共生菌 Candidatus Dactylopiibacterium carminicum 的比较基因组学揭示了通过 CRISPR/Cas 保护宿主免受病毒侵害的可能性。","authors":"Rafael Bustamante-Brito , Arturo Vera-Ponce de León , Mónica Rosenblueth , Esperanza Martínez-Romero","doi":"10.1016/j.syapm.2024.126540","DOIUrl":null,"url":null,"abstract":"<div><p>We present new genomes from the bacterial symbiont <em>Candidatus</em> Dactylopiibacterium carminicum obtained from non-domesticated carmine cochineals belonging to the scale insect <em>Dactylopius</em> (Hemiptera: Coccoidea: Dactylopiidae). As <em>Dactylopiibacterium</em> has not yet been cultured in the laboratory, metagenomes and metatranscriptomics have been key in revealing putative symbiont functions. <em>Dactylopiibacterium</em> is a nitrogen-fixing beta-proteobacterium that may be vertically transmitted and shows differential gene expression inside the cochineal depending on the tissue colonized. Here we found that all cochineal species tested had <em>Dactylopiibacterium carminicum</em> which has a highly conserved genome. All <em>Dactylopiibacterium</em> genomes analyzed had genes involved in nitrogen fixation and plant polymer degradation. <em>Dactylopiibacterium</em> genomes resemble those from free-living plant bacteria, some found as endophytes. Notably, we found here a new putative novel function where the bacteria may protect the insect from viruses, since all <em>Dactylopiibacterium</em> genomes contain CRISPRs with a spacer matching nucleopolyhedrovirus that affects insects.</p></div>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative genomics of the carmine cochineal symbiont Candidatus Dactylopiibacterium carminicum reveals possible protection to the host against viruses via CRISPR/Cas\",\"authors\":\"Rafael Bustamante-Brito , Arturo Vera-Ponce de León , Mónica Rosenblueth , Esperanza Martínez-Romero\",\"doi\":\"10.1016/j.syapm.2024.126540\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We present new genomes from the bacterial symbiont <em>Candidatus</em> Dactylopiibacterium carminicum obtained from non-domesticated carmine cochineals belonging to the scale insect <em>Dactylopius</em> (Hemiptera: Coccoidea: Dactylopiidae). As <em>Dactylopiibacterium</em> has not yet been cultured in the laboratory, metagenomes and metatranscriptomics have been key in revealing putative symbiont functions. <em>Dactylopiibacterium</em> is a nitrogen-fixing beta-proteobacterium that may be vertically transmitted and shows differential gene expression inside the cochineal depending on the tissue colonized. Here we found that all cochineal species tested had <em>Dactylopiibacterium carminicum</em> which has a highly conserved genome. All <em>Dactylopiibacterium</em> genomes analyzed had genes involved in nitrogen fixation and plant polymer degradation. <em>Dactylopiibacterium</em> genomes resemble those from free-living plant bacteria, some found as endophytes. Notably, we found here a new putative novel function where the bacteria may protect the insect from viruses, since all <em>Dactylopiibacterium</em> genomes contain CRISPRs with a spacer matching nucleopolyhedrovirus that affects insects.</p></div>\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-07-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202024000547\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202024000547","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Comparative genomics of the carmine cochineal symbiont Candidatus Dactylopiibacterium carminicum reveals possible protection to the host against viruses via CRISPR/Cas
We present new genomes from the bacterial symbiont Candidatus Dactylopiibacterium carminicum obtained from non-domesticated carmine cochineals belonging to the scale insect Dactylopius (Hemiptera: Coccoidea: Dactylopiidae). As Dactylopiibacterium has not yet been cultured in the laboratory, metagenomes and metatranscriptomics have been key in revealing putative symbiont functions. Dactylopiibacterium is a nitrogen-fixing beta-proteobacterium that may be vertically transmitted and shows differential gene expression inside the cochineal depending on the tissue colonized. Here we found that all cochineal species tested had Dactylopiibacterium carminicum which has a highly conserved genome. All Dactylopiibacterium genomes analyzed had genes involved in nitrogen fixation and plant polymer degradation. Dactylopiibacterium genomes resemble those from free-living plant bacteria, some found as endophytes. Notably, we found here a new putative novel function where the bacteria may protect the insect from viruses, since all Dactylopiibacterium genomes contain CRISPRs with a spacer matching nucleopolyhedrovirus that affects insects.