胭脂鱼共生菌 Candidatus Dactylopiibacterium carminicum 的比较基因组学揭示了通过 CRISPR/Cas 保护宿主免受病毒侵害的可能性。

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Rafael Bustamante-Brito , Arturo Vera-Ponce de León , Mónica Rosenblueth , Esperanza Martínez-Romero
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引用次数: 0

摘要

我们展示了从属于鳞翅目昆虫胭脂虫(半翅目:茧科:Dactylopiidae)的非驯化胭脂虫身上获得的细菌共生体胭脂虫细菌(Candidatus Dactylopiibacterium carminicum)的新基因组。由于 Dactylopiibacterium 尚未在实验室中培养,因此元基因组和元转录组学是揭示假定共生功能的关键。Dactylopiibacterium 是一种固氮的 beta 蛋白细菌,可以垂直传播,并根据定殖组织的不同,在褐藻体内表现出不同的基因表达。在这里,我们发现所有被检测的褐藻物种都含有褐藻双歧杆菌,而褐藻双歧杆菌的基因组具有高度保守性。分析的所有 Dactylopiibacterium 基因组都有参与固氮和植物聚合物降解的基因。Dactylopiibacterium 的基因组与自由生活的植物细菌的基因组相似,其中一些是作为内生菌发现的。值得注意的是,我们在这里发现了一种新的推测功能,即细菌可能会保护昆虫免受病毒的侵害,因为所有的双歧杆菌基因组都含有与影响昆虫的核多角体病毒相匹配的CRISPRs。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative genomics of the carmine cochineal symbiont Candidatus Dactylopiibacterium carminicum reveals possible protection to the host against viruses via CRISPR/Cas

We present new genomes from the bacterial symbiont Candidatus Dactylopiibacterium carminicum obtained from non-domesticated carmine cochineals belonging to the scale insect Dactylopius (Hemiptera: Coccoidea: Dactylopiidae). As Dactylopiibacterium has not yet been cultured in the laboratory, metagenomes and metatranscriptomics have been key in revealing putative symbiont functions. Dactylopiibacterium is a nitrogen-fixing beta-proteobacterium that may be vertically transmitted and shows differential gene expression inside the cochineal depending on the tissue colonized. Here we found that all cochineal species tested had Dactylopiibacterium carminicum which has a highly conserved genome. All Dactylopiibacterium genomes analyzed had genes involved in nitrogen fixation and plant polymer degradation. Dactylopiibacterium genomes resemble those from free-living plant bacteria, some found as endophytes. Notably, we found here a new putative novel function where the bacteria may protect the insect from viruses, since all Dactylopiibacterium genomes contain CRISPRs with a spacer matching nucleopolyhedrovirus that affects insects.

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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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