Skin Pharmacology and Physiology最新文献

{"title":"Intraday Variations in Skin Water Parameters.","authors":"Harvey N Mayrovitz","doi":"10.1159/000541547","DOIUrl":"10.1159/000541547","url":null,"abstract":"<p><strong>Introduction: </strong>Three interrelated skin water assessments include stratum corneum hydration (SCH) via electrical measurements, skin water using tissue dielectric constant (TDC) measurements, and transepidermal water loss (TEWL). These are differentially used for skin physiology research, clinical assessments of dermatological conditions and to assess skin water in diabetes and lymphedema. Often volar forearm skin is used for assessments done at various times of day (TOD). The present goal was to assess the extent of intraday variability in SCH, TDC, and TEWL.</p><p><strong>Methods: </strong>Twelve medical students self-measured SCH, TDC, and TEWL on their forearm every 2 h from 08:00 to 24:00 h on 2 consecutive days. All participants were well trained and pre-certified in all procedures. Tests for parameter differences among TOD were via the nonparametric Friedman test.</p><p><strong>Results: </strong>No significant differences in SCH or TEWL were found among TOD over the 16-h interval for either day or combined. Contrastingly, TDC decreased slightly but significantly from morning through evening. There was no evidence of a diurnal pattern. Interestingly, a significant nonlinear relationship between TEWL and SCH was detected.</p><p><strong>Conclusion: </strong>Findings indicate only minor intraday variations with TOD trend except for TDC which decreases slightly from morning through evening. The clinical relevance relates to the confidence now gained associated with the parameter estimates when measured at different TOD during normal clinic hours or beyond. This should help in estimating the potential importance of small differences if measured at a different TOD. From a physiological viewpoint, the findings uncover and describe an interesting nonlinear relationship between TEWL and SCH which may serve to propel further investigations that might better characterize this process.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"80-91"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etiological Insights of Acne in Atopic Dermatitis Patients under Upadacitinib Treatment: An Exploratory Study.","authors":"Guilherme Muzy","doi":"10.1159/000539207","DOIUrl":"10.1159/000539207","url":null,"abstract":"","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"59-62"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140863860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo Study to Evaluate an Intelligent Algorithm for Time Efficient Detection of Malignant Melanoma Using Dermatofluoroscopy.","authors":"Karl Weihmann, Johannes Schleusener, Thomas K Eigentler, Franziska C Ghoreschi, Rose K C Moritz, Lukasz Szyc, Eduard Dronnik, Martina C Meinke","doi":"10.1159/000542854","DOIUrl":"10.1159/000542854","url":null,"abstract":"<p><strong>Introduction: </strong>Dermatofluoroscopy is an optical noninvasive method of melanoma/nevus differentiation that has shown 89% sensitivity and 45% specificity in clinical trials, but long measurement duration hinders clinical use. An intelligent algorithm was developed to shorten the measurement time without compromising its diagnostic accuracy. It uses dermoscopic images of the skin lesions to be measured to select measurement points based on the assessment of color values.</p><p><strong>Methods: </strong>27 patients with a total of 29 lesions suggestive of cutaneous melanoma were included in a clinical study and measured with both methods, conventional dermatofluoroscopy and the newly developed intelligent algorithm. The results were compared to the independent findings of two histopathologists to evaluate diagnostic accuracy and time saved.</p><p><strong>Results: </strong>There was a median reduction of measurement points from 265 to 158 (40%). Meanwhile, the intelligent algorithm showed a higher diagnostic accuracy than conventional dermatofluoroscopy (area under the curve of 72% vs. 63%).</p><p><strong>Conclusion: </strong>The intelligent algorithm did not perform inferior to the conventional method while saving 40% of time. However, measurement times remain long compared to other noninvasive methods of diagnosing malignant melanoma. Further studies are needed to evaluate clinical suitability.</p><p><strong>Introduction: </strong>Dermatofluoroscopy is an optical noninvasive method of melanoma/nevus differentiation that has shown 89% sensitivity and 45% specificity in clinical trials, but long measurement duration hinders clinical use. An intelligent algorithm was developed to shorten the measurement time without compromising its diagnostic accuracy. It uses dermoscopic images of the skin lesions to be measured to select measurement points based on the assessment of color values.</p><p><strong>Methods: </strong>27 patients with a total of 29 lesions suggestive of cutaneous melanoma were included in a clinical study and measured with both methods, conventional dermatofluoroscopy and the newly developed intelligent algorithm. The results were compared to the independent findings of two histopathologists to evaluate diagnostic accuracy and time saved.</p><p><strong>Results: </strong>There was a median reduction of measurement points from 265 to 158 (40%). Meanwhile, the intelligent algorithm showed a higher diagnostic accuracy than conventional dermatofluoroscopy (area under the curve of 72% vs. 63%).</p><p><strong>Conclusion: </strong>The intelligent algorithm did not perform inferior to the conventional method while saving 40% of time. However, measurement times remain long compared to other noninvasive methods of diagnosing malignant melanoma. Further studies are needed to evaluate clinical suitability.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"97-103"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stratum Corneum Hydration Measurements with a Bluetooth Wireless Probe: A Real-Life Study at Home Compared to Measurements under Laboratory Conditions.","authors":"Joachim W Fluhr, Agnès Voisard, Dessyslava G Nikolaeva, Leonie Herzog, Georg Wiora, Jeremias Gayer, Nicolas J Lehmann, Razvigor Darlenski","doi":"10.1159/000539411","DOIUrl":"10.1159/000539411","url":null,"abstract":"<p><strong>Introduction: </strong>Non-invasive measurement of the stratum corneum hydration (SCH) with capacitance-based instrumentation is established in dermatological and cosmetic studies. We wanted to test the reliability of non-invasive self-measurements for SCH performed under real-life conditions by volunteers with a Bluetooth-based (wireless) probe Corneometer® (CM 825i) transmitting the data via a smartphone application to a central server. Probes and smartphones communicated using Bluetooth Low Energy. Data from the smartphone were securely transferred to a remote server in a different country with TLS encryption using HTTPS protocols. CM 825i values were correlated with the established CM 825 under laboratory conditions. The primary endpoint was the correlation of the two probes. Secondary endpoints were the coefficient of variation (CV) and delta values (before and after treatment).</p><p><strong>Methods: </strong>Eighteen healthy volunteers (f: 8; m: 10) participated in the prospective observational study. The real-world home use of the wireless CM 825i was performed before and after treatments with base cream DAC for 7 days.</p><p><strong>Results: </strong>Both instruments showed a significant and relevant correlation (p &lt; 0.0001; Spearman coefficient of r = 0.8647). CM 825i and CM 825 differentiate significantly between normal and high SCH. Both devices showed comparable robustness in repeated measurements with a CV between 5.6% and 9.2%.</p><p><strong>Conclusion: </strong>We could show a significant correlation between both devices and a comparable differentiation between low and high SCH and comparable CVs. The real-life use demonstrated adequate acquiring and transmitting of in vivo data to a smartphone and subsequently transmitting to the secure server with low numbers of missed transmissions (&lt;0.2%) and missed measurements (&lt;5%).</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"40-48"},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11488832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front & Back Matter","authors":"J. Fluhr, M. Lane","doi":"10.1159/000531338","DOIUrl":"https://doi.org/10.1159/000531338","url":null,"abstract":"","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47969415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front & Back Matter","authors":"J. Fluhr, M. Lane","doi":"10.1159/000531033","DOIUrl":"https://doi.org/10.1159/000531033","url":null,"abstract":"","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":"174 ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41283264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front & Back Matter","authors":"J. Fluhr, M. Lane","doi":"10.1159/000529749","DOIUrl":"https://doi.org/10.1159/000529749","url":null,"abstract":"","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49328215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Mucopolysaccharide Polysulfate on Steroid-Induced Tight Junction Barrier Dysfunction in Human Epidermal Keratinocytes and a 3D Skin Model.","authors":"Akira Koda, Yuko Ishii, Ayu Kashiwagi, Mika Fujikawa, Keisuke Kikuchi, Ryota Hashimoto, Yuhki Ueda, Takaaki Doi","doi":"10.1159/000529962","DOIUrl":"10.1159/000529962","url":null,"abstract":"<p><strong>Introduction: </strong>The long-term use of topical corticosteroids (TCS) is associated with side effects such as skin atrophy and barrier deterioration. Moisturizers, such as mucopolysaccharide polysulfate (MPS), have been reported to prevent relapses in atopic dermatitis (AD) when used in combination with TCS. However, the mechanisms underlying the positive effects of MPS in combination with TCS in AD are poorly understood. In the present study, we investigated the effects of MPS in combination with clobetasol 17-propionate (CP) on tight junction (TJ) barrier function in human epidermal keratinocytes (HEKa) and 3D skin models.</p><p><strong>Methods: </strong>The expression of claudin-1, which is crucial for TJ barrier function in keratinocytes, and transepithelial electrical resistance (TEER) was measured in CP-treated human keratinocytes incubated with and without MPS. A TJ permeability assay, using Sulfo-NHS-Biotin as a tracer, was also conducted in a 3D skin model.</p><p><strong>Results: </strong>CP reduced claudin-1 expression and TEER in human keratinocytes, whereas MPS inhibited these CP-induced effects. Moreover, MPS inhibited the increase in CP-induced TJ permeability in a 3D skin model.</p><p><strong>Conclusion: </strong>The present study demonstrated that MPS improved TJ barrier impairment induced by CP. The improvement of TJ barrier function may partially be responsible for the delayed relapse of AD induced by the combination of MPS and TCS.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"186-194"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10652656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9174884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Phase I Study to Evaluate the Safety and Pharmacokinetics of SHR0302 Base Ointment in Healthy Adult Volunteers.","authors":"Baoping Li,&nbsp;Nanyang Li,&nbsp;Aik Han Goh,&nbsp;Haifeng Cao,&nbsp;Min Wang Irwin,&nbsp;Xunian Tong,&nbsp;Jingjing Wang,&nbsp;Xiaojie Wu,&nbsp;Jing Zhang,&nbsp;Dan Pu","doi":"10.1159/000528739","DOIUrl":"https://doi.org/10.1159/000528739","url":null,"abstract":"<p><strong>Introduction: </strong>SHR0302 is a highly selective JAK1 inhibitor. This study aimed to investigate the safety, tolerability, and pharmacokinetics of single and multiple-dose topical skin application of SHR0302 base ointment in healthy adult subjects.</p><p><strong>Methods: </strong>This phase I clinical trial (registration number: CTR20192188) consisted of two parts. Part 1 was a single-dose ascending study with four dose levels in 32 healthy Australian adults (8 subjects in each dose group). All Australian subjects were randomized 3:1 to a single-dose topical skin application of SHR0302 base ointment or placebo. The dose escalated from 1% SHR0302 base ointment on 3% of body surface area (BSA) to 2% SHR0302 base ointment on 20% of BSA. Part 2 combined single and multiple-dose ascension studies with two dose levels in 20 healthy Chinese adults (10 subjects in each dose group). All Chinese subjects were randomized 4:1 to a combination of single and multiple doses for consecutive 10 days of topical application of 1% SHR0302 base ointment on 20% BSA or 2% SHR0302 base ointment on 20% BSA. The safety and pharmacokinetics of the SHR0302 base ointment were evaluated.</p><p><strong>Results: </strong>The incidence of treatment-emergent adverse events (TEAEs) in both parts was comparable between the SHR0302 base ointment group and the vehicle group (part 1: 33.3% vs. 37.5%; part 2: 56.3% vs. 75.0%). All TEAEs were transient, recovered, and equally well-tolerated in the two racial groups. The overall absorption of the SHR0302 base ointment was slow after topical application, with Tmax>10 h. After a single dose of the SHR0302 base ointment, drug exposure in healthy Australian and Chinese subjects increased nonlinearly with the increase in the administration area and drug content. Drug exposure increased in a less-than-dose-proportional manner within the dose range tested. Due to differences in the clinical practice of topical application, the Tmax of the drug in Australian subjects was earlier than in Chinese subjects, but the overall extent of absorption seemed comparable in Australian and Chinese subjects (with comparable AUC0-t).</p><p><strong>Conclusion: </strong>The SHR0302 base ointment (either single or multiple doses) was well tolerated and safe, with no racial disparity.</p><p><strong>Key message: </strong>The SHR0302 base ointment (either single or multiples doses) was well tolerated and safe.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":"36 2","pages":"76-86"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9532858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Epidermal Environment's Influence on the Dermal Environment in Response to External Stress.","authors":"Masaki Yoshida,&nbsp;Kyong-Oh Shin,&nbsp;Sora Muraoka,&nbsp;Yerim Choi,&nbsp;Jae-Ho Park,&nbsp;Soo-Hyun Park,&nbsp;Jin-Taek Hwang,&nbsp;Kyungho Park,&nbsp;Yoshikazu Uchida","doi":"10.1159/000529743","DOIUrl":"https://doi.org/10.1159/000529743","url":null,"abstract":"<p><strong>Introduction: </strong>The outermost layer of the skin, the epidermis, is directly exposed to external stress (e.g., irradiation, allergens, and chemicals). Changes in epidermal conditions/environment in response to this stress could also influence conditions of the dermis, located directly beneath the epidermis. Yet, whether/how any epidermal environment changes in response to external stress affect dermal functions has not been completely clarified.</p><p><strong>Methods: </strong>We employed ultraviolet irradiation B (UVB) (which hardly reaches the dermis) as a model of external stress. Human keratinocytes and human dermal fibroblasts were treated with UVB and conditioned medium of keratinocytes exposed to UVB (UVB-keratinocyte-M), respectively. We assessed (1) inflammatory cytokines and lipid mediators in keratinocytes; (2) matrix metalloprotease (MMP) levels and collagen degradation in fibroblasts; (3) ex vivo organ-cultured human skin was treated with UVB. MMP levels and collagen degradation were examined; (4) test whether the mixture of agent (agent cocktail) consisting of dihydroceramide, niacin amide, resveratrol, glucosyl hesperidin, and phytosterol ester that has been shown to improve skin barrier integrity can mitigate influence of UVB in skin; and (5) a pilot one-arm human clinical test to assess efficacy of formulation containing agent cocktail on stratum corneum hydration, skin elasticity, and wrinkle index.</p><p><strong>Results: </strong>Inflammatory-cytokine and -lipid mediator production were increased in cultured keratinocytes treated with UVB, while matrix MMP-1, -3, and -9 production and collagen degradation were increased in fibroblasts incubated with UVB-keratinocyte-M. mRNA expression of COL1A1 (that codes type 1 collagen) levels was decreased in fibroblasts incubated with UVB-keratinocyte-M. The study using ex vivo organ-cultured human skin showed both MMP-1 and MMP-9 expression were increased in both epidermis and dermis and increased dermal collagen degradation following UVB irradiation. Increased MMP production and collagen degradation were attenuated by application of an agent cocktail. Finally, a pilot clinical study demonstrated that the formulation containing our agent cocktail likely has the ability to improve skin hydration, increase skin elasticity, and reduce the appearance of wrinkles.</p><p><strong>Conclusion: </strong>Epidermal changes in epidermal environment and conditions in response to external stress affect dermal conditions, and these negative effects of external stress on various skin layers can be pharmacologically mitigated.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":"36 3","pages":"149-159"},"PeriodicalIF":2.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9562425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}