Skin Pharmacology and Physiology最新文献

{"title":"Effects of Two Different Basic Skincare Regimens on Children and Adult Skin Microbiota: An Exploratory Randomized Controlled Crossover Trial.","authors":"Kathrin Hillmann, Tsenka Tomova-Simitchieva, Pauline Sophia Pinta, Zhile Xiong, Annette Moter, Varvara Kanti-Schmidt, Jan Kottner, Ulrike Blume-Peytavi","doi":"10.1159/000545433","DOIUrl":"10.1159/000545433","url":null,"abstract":"<p><strong>Introduction: </strong>Regular use of leave-on skincare products has positive effects on the skin barrier and appears to influence the skin microbiome in atopic dermatitis (AD). However, product-related effects on the skin microbiome are unclear. The aim of this study was to investigate to which extent the application of an emollient plus product differs from a petrolatum-based basic skincare product in terms of skin physiology, skin barrier, and skin microbiome.</p><p><strong>Methods: </strong>In an exploratory study regime, 50 subjects (children and adults) with mild AD were randomized to receive Bepanthen SensiDaily® (BSD) and Basiscreme DAC (DAC) for 3 months in a crossover design. Skin physiological measurements and skin microbiome swabs were taken on the forearm and lower leg before and after each 3-month application period of the respective product.</p><p><strong>Results: </strong>Improvement of skin physiological parameters after 3 months was observed in both topical leave-on product groups. Statistically significant differences were observed in baseline microbiomes between children and adults (p < 0.001) but not between the product groups.</p><p><strong>Conclusion: </strong>Regular application of leave-on products leads to better stratum corneum hydration after 3 months with better effects of BSD on the forearms of children and the lower legs of adults compared to DAC. The application of BSD and DAC did not lead to significant alterations in the overall composition of the skin microbiome. Although there were shifts in the frequency of certain microbial genera, these changes were not consistent between age groups and treatments.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"103-112"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Pharmacokinetic Parameters from in vitro Permeation Test Data for Predicting Multiple-Dose Penetration Profiles.","authors":"Paul A Lehman","doi":"10.1159/000546732","DOIUrl":"10.1159/000546732","url":null,"abstract":"<p><strong>Introduction: </strong>Several complex mathematical models have been developed using in vitro permeation test (IVPT) data to characterize percutaneous absorption. A less complicated approach, using basic pharmacokinetic parameters on IVPT data, is proposed here to predict skin barrier content and permeation kinetics following multiple-dose applications.</p><p><strong>Methods: </strong>Published and archived data from the authors' files are used to define and test a proposed model using standard single-compartment pharmacokinetic parameters and to provide insight into percutaneous absorption profiles and skin barrier content.</p><p><strong>Results: </strong>Pharmacokinetic parameters are derived and shown for a selection of diverse drugs from their IVPT data, which are then used to predict multiple-dose absorption kinetics. Flux profiles and skin barrier content are calculated and shown for periods of 7-30 days with 6-, 12-, and 24-h dosing intervals.</p><p><strong>Conclusion: </strong>The model presented here allows one to predict the rate and extent of drug absorption over any number of dosing periods per day and across multiple days. This information may not only provide a new outlook on formulation selection or dosing regimens, but may also allow for estimation of skin or systemic levels of exposure to chemicals following multiple sequential topical dose applications.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"127-137"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Artificial Intelligence for Dermatological Care during Space Missions.","authors":"Diala Haykal, Mohamad Goldust","doi":"10.1159/000546730","DOIUrl":"10.1159/000546730","url":null,"abstract":"","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"172-174"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intake of Soymilk-Okara Powder for 8 Weeks Induced the Improvement of Skin Elasticity in Japanese Women.","authors":"Akihiro Maeta, Masahiro Katsukawa, Yaeko Hayase, Kyoko Takahashi","doi":"10.1159/000543802","DOIUrl":"10.1159/000543802","url":null,"abstract":"<p><strong>Introduction: </strong>Soymilk okara, a rich source of protein and dietary fiber, is a byproduct of soymilk manufacturing. We investigated whether soymilk-okara intake improved skin conditions in Japanese women with self-reported constipation.</p><p><strong>Methods: </strong>Thirty-seven Japanese women with self-reported constipation were included in this study. Two-thirds of the participants ingested 15 g of soymilk-okara powder daily for 8 weeks (okara group), whereas seasonal effects were evaluated in the remaining one-third (observation group). The participants' body composition and skin conditions (stratum corneum hydration, transepidermal water loss [TEWL], and gross elasticity [R2; epidermis and dermis]) of the malar and forehead were measured. Additionally, fecal concentrations of uremic toxins such as indole and p-cresol were analyzed.</p><p><strong>Results: </strong>Eight participants withdrew consent during the study period owing to COVID-19, etc., and the final data analysis was performed using data from participants in the okara (n = 19) and observation (n = 10) groups. No significant interactions among stratum corneum hydration, TEWL, or epidermal gross elasticity of the malar and forehead in the okara and observation groups were observed. In contrast, interactions of the dermis gross elasticity of the malar and forehead in both groups (p = 0.065 and 0.043, respectively) were observed. In the okara group, negative correlations between the changes in uremic toxins and the difference in the dermis gross elasticity of the forehead were observed.</p><p><strong>Conclusion: </strong>The intake of soymilk-okara powder improved skin elasticity, which may be a result of changes in the intestinal flora.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"35-44"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-211 Regulates Cutaneous Wound Healing through Inhibiting Inflammatory Reactions and Oxidative Stress by Binding SOX11.","authors":"Yun Chen, Xinyi Zhang, Fangfang Wu, Lixia Wang, Hongju Zuo, Hanbing Tian, Huan Chen","doi":"10.1159/000542697","DOIUrl":"10.1159/000542697","url":null,"abstract":"<p><strong>Introduction: </strong>Loss of skin integrity due to a wound or disease can lead to severe disability or even life threat. The highly expressed microRNAs in the skin are of great significance for skin development. The purpose of the investigation was to explore the effect and mechanism of miR-211 on inflammation, oxidative stress, and migration in keratinocytes.</p><p><strong>Methods: </strong>The HaCaT keratinocytes were treated with hydrogen peroxide (H2O2) to establish a wound-healing model. The expression of miR-211 was examined by quantitative real-time PCR. The cell function was reflected in proliferative ability, migration, apoptosis, and inflammation, which were evaluated using the Cell Counting Kit-8 (CCK-8) assay, transwell test, flow cytometry technique, and enzyme-linked immunosorbent assay (ELISA). The target of miR-211 was verified by luciferase luminescence measurements.</p><p><strong>Results: </strong>H2O2 inhibited HaCaT cell proliferation, migration, and promoted cell apoptosis, accompanied with the downregulation of miR-211. H2O2 led to inflammatory response and oxidative damage to HaCaT. miR-211 promoted proliferation and migration but improved cell apoptosis of HaCaT. The role of H2O2 on inflammatory response and oxidative stress was alleviated by miR-211. SRY-box transcription factor 11 (SOX11) was a targeted mediator of miR-211. SOX11 reversed the influence of miR-211 on cell proliferation, migration, apoptosis, inflammatory response, and oxidative stress.</p><p><strong>Conclusion: </strong>miR-211 regulated the proliferation, migration, apoptosis, inflammation, and oxidative stress of keratinocytes by mediating SOX11, thus participating in cutaneous wound healing.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"10-20"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healing Diabetic Foot Ulcers with Topical Timolol Improves Healed Epithelial Integrity.","authors":"Rawlings E Lyle, Mirabel E Dafinone, Pallas Lim, Anuj Budhiraja, Alisha Mehta, Sara E Dahle, Roslyn Rivkah Isseroff","doi":"10.1159/000545357","DOIUrl":"10.1159/000545357","url":null,"abstract":"<p><strong>Introduction: </strong>Diabetic foot ulcers (DFUs) are a common complication in diabetes, leading to high amputation risk and significant healthcare costs. Given topical timolol's emergence as a potential wound-healing agent, our study explored its impact on epidermal integrity.</p><p><strong>Methods: </strong>This study was a post hoc analysis conducted as part of a randomized controlled trial at the Veterans Affairs Northern California Health Care System. Twenty patients, who had DFUs healed in the original trial, 10 in the timolol arm, and 10 in the placebo arm, were enrolled in the study. The primary outcome was transepidermal water loss, measured monthly for 3 months of post-healing using a closed-chamber device. The secondary outcome was re-ulceration rates over 1 year.</p><p><strong>Results: </strong>Transepidermal water loss at 1, 2, and 3 months of post-healing was significantly lower in the timolol group than in the placebo group (p < 0.01). Linear mixed models identified contralateral foot transepidermal water loss as a significant predictor of healed diabetic foot ulcer site transepidermal water loss (estimate = 0.76, p < 0.001). The interaction between timolol treatment and months since healing significantly reduced transepidermal water loss over time (estimate = -2.2, p = 0.002). The use of a wheelchair was also associated with a significant decrease in transepidermal water loss (estimate = -7.7, p = 0.01). Initial transepidermal water loss values were higher in patients who re-ulcerated, but the difference was not statistically significant (p = 0.42). There was no difference in re-ulceration rates in this small pilot study.</p><p><strong>Conclusion: </strong>Topical timolol significantly improved skin barrier function in healed DFUs, reducing transepidermal water loss. Although re-ulceration rates were not significantly different, the trend suggests potential benefits. Further studies with larger sample sizes and longer follow-up are needed to confirm these findings and explore transepidermal water loss's predictive value for re-ulceration.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"92-102"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Reactive Oxygen Species in the Pathogenesis of Alopecia Areata: A Systematic Review and Meta-Analysis.","authors":"Jonah Perlmutter, Polycronis P Akouris, Sierra Fremont, Brian Yang, Evan Toth, Michael Eze, Marni Wiseman","doi":"10.1159/000543373","DOIUrl":"10.1159/000543373","url":null,"abstract":"<p><strong>Background: </strong>Alopecia areata (AA) is a T-cell-mediated autoimmune disease that significantly impacts patient quality of life. The breakdown of hair follicle immune privilege underlies AA pathogenesis. However, the precise mechanism of this breakdown remains unclear. This study investigates the potential role of reactive oxygen species in AA pathogenesis.</p><p><strong>Summary: </strong>A systematic review and meta-analysis were conducted on observational studies and randomized controlled trials from 2000 to 2024. Studies included AA patients and measured oxidative stress index (OSI), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), or paraoxonase-1 (PON1). Extracted data were analyzed using the Cochrane risk-of-bias tool and random-effects models. The review included 21 studies with 743 AA patients. OSI was elevated in AA patients (effect size = 1.58, 95% CI: 0.31-2.68, p = 0.00068). MDA levels were also elevated (effect size = 1.60, 95% CI: 0.43-2.6, p = 0.00023), while SOD (effect size = -0.97, 95% CI: -1.65 to -0.30, p = 0.00066) and GSH-Px (effect size = -1.41, 95% CI: -2.28 to -0.53, p = 0.00068) activities were reduced. PON1 levels showed no significant difference (effect size = -3.56, 95% CI: -8.63 to 1.51, p = 0.051).</p><p><strong>Key messages: </strong>The elevated OSI and MDA, and decreased antioxidant activity in AA patients suggest a substantial role for reactive oxygen species and oxidative stress in AA pathogenesis, highlighting oxidative stress as a potential target for therapeutic intervention. These results underscore the importance of oxidative stress in AA and support further research into antioxidant-based therapies.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"59-67"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How to Choose an Emollient? Pharmaceutical and Sensory Attributes for Product Selection.","authors":"Petra Huber, Daphne Reinau, Zoé Brodard, Christoph R Meier, Christian Surber","doi":"10.1159/000543653","DOIUrl":"10.1159/000543653","url":null,"abstract":"<p><strong>Introduction: </strong>Emollients are part of daily body care and have become indispensable therapeutic adjuvants for the treatment of dry skin conditions. Adherence to topical treatments, notably for dry skin conditions, has been reported to be low. The underlying reasons may include insufficient medical and nursing support for product selection, specific product attributes, aspects of product application, and product feel on the skin. Attempts have also been made to portray lipid content, galenic product format, or rheological attributes (pharmaceutical attributes) as adherence-promoting or adherence-preventing properties. In the treatment of dry dermatoses with emollients, there is little information describing and relating to these various features. We explored whether the sensory attributes of selected emollients were associated with common product attributes such as lipid content, viscosity, or galenic product format and discuss the extent to which this information is useful for product selection.</p><p><strong>Methods: </strong>Nine trained panellists evaluated ten selected emollients based on a set of 18 predefined sensory attributes according to a standard guide for sensory descriptive analysis. Viscosity was determined using a rotational rheometer.</p><p><strong>Results: </strong>The emollients had product-specific sensory attributes. Lipid content, viscosity, and galenic product format are not generally indicative of sensory product attributes.</p><p><strong>Conclusion: </strong>Contrary to popular belief, lipid content and viscosity are not generally indicative of sensory product attributes. This is mainly due to the different physicochemical properties of the lipid-phase ingredients, which are product-specific and diverse. As most emollients contain significant amounts of volatile ingredients that evaporate during and after application, their galenic format changes dramatically. Therefore, this is not a viable selection criterion. Because refined information on sensory product attributes, as compiled for this study, is rarely available in everyday life, eliciting individual and subjective patient preferences through dialogue remains crucial. Ideally, patient preferences can be elicited from the sample packs.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"1-9"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12136608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143011101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reflectance Confocal Microscopy of Dermal Papillae in Healthy Skin: A Histopathology Controlled Study.","authors":"Francesco Lacarrubba, Anna Elisa Verzì, Cosimo Misciali, Davide Domenicali, Giuseppe Micali","doi":"10.1159/000543491","DOIUrl":"10.1159/000543491","url":null,"abstract":"<p><strong>Introduction: </strong>Previous studies have investigated the density of dermal papillae (DP) in normal skin using reflectance confocal microscopy (RCM), a noninvasive imaging technique that allows a real-time, high-resolution imaging of the skin, although no histological confirmation was provided. The aim of the present study was to compare the RCM evaluation of DP density in healthy skin with horizontal histopathological sections (HHS), a technique that provides a horizontal view of the skin.</p><p><strong>Method: </strong>Ten adult patients were selected, and a healthy skin area was marked for RCM examination and a subsequent 5-mm punch biopsy that was processed for HHS. Two different blinded operators performed DP counting on RCM and HHS images, respectively.</p><p><strong>Results: </strong>A total of 10 skin samples were obtained from the lower back. The mean DP density resulting from RCM was 84.27 ± 3.24/mm2, while that from HHS was 84.08 ± 2.74/mm2. Student t test showed no significant differences in DP count between the two techniques (p = 0.89).</p><p><strong>Discussion: </strong>The strength of this study is represented by the histological evaluation which has never been previously performed, whose results align with the RCM findings and validate previous data from our group, with negligible differences. We believe that the exact identification of the DP number in normal skin may have practical implications, as several inflammatory skin conditions are characterized by DP changes such as psoriasis, lichen planus, and discoid lupus.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"76-78"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143011109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between Skin Structure and Skin Function of Pregnant Women and Their Infants: A Prospective Cohort Study.","authors":"Doris Wilborn, Agathe Franz, Gabriela Engelhardt, Tsenka Tomova-Simitchieva, Andria Constantinou, Ruhul Amin, Kathrin Hillmann, Jan Kottner, Gavin Zhou, Ulrike Blume-Peytavi","doi":"10.1159/000546770","DOIUrl":"10.1159/000546770","url":null,"abstract":"<p><p><p>Introduction: In women during pregnancy and in infants during the first months after birth, skin health is challenged. However, evidence about the structural and functional changes of the skin during and after pregnancy is largely lacking.</p><p><strong>Methods: </strong>The first prospective cohort study was conducted, following women from pregnancy through the postpartum period and their infants until 6 months of age, with skin structure and function measured at different time points. Due to the explorative character of the study, descriptive statistics were used.</p><p><strong>Results: </strong>Over the study period, transepidermal water loss, epidermal thickness, and skin roughness in women increased. Pregnancy and postpartum period affected skin parameters such as skin roughness, epidermal thickness, and transepidermal water loss, whereas stratum corneum hydration, pH, skin stiffness, and skin elasticity were not affected in women. Infants' skin barrier function matched literature values for healthy skin, with roughness and dryness decreasing through 6 months of age. Infants' skin barrier function characteristics matched literature values for healthy skin, with skin roughness and dryness decreasing by 6 months of age.</p><p><strong>Conclusion: </strong>Based on the findings of this observational cohort study, we found no statistically significant correlation between maternal health and skin characteristics and skin characteristics of infants except for women's skin roughness and infants' skin stiffness and skin elasticity and women's skin stiffness and skin elasticity and infants' skin elasticity. Therefore, based on our findings it may be justified to consider using skin care for maintaining barrier quality and function: (a) in pregnant women with a positive effect on skin roughness and transepidermal water loss and (b) in infants improving dry skin and skin roughness. </p>.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"138-148"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12240566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}