Skin Pharmacology and Physiology最新文献

{"title":"Epidermal Homeostasis in Space from the Stand Point of Keratinocyte Physiology.","authors":"Mitsuhiro Denda","doi":"10.1159/000547984","DOIUrl":"https://doi.org/10.1159/000547984","url":null,"abstract":"<p><p>The human epidermis is the interface between the　human body and the environment. It is mainly comprised of keratinocytes. Since the beginning of this century, functional expression of a series of receptors for various environmental factors has been reported. Moreover, neurotransmitters and hormones originally found in the central nervous system are generated and released from keratinocytes. Thus, the pathophysiology of the epidermis is important not only for skin health but also for whole-body conditions. In outer space, humans must confront environmental factors different from those on Earth, such as electromagnetic fields, drastic changes in atmospheric pressure or humidity, and microgravity. In this review, the author discusses the potential effects of these factors on epidermal homeostasis and whole-body conditions.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"1-11"},"PeriodicalIF":3.2,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative Stress-Mediated Modulation of Fibrosis and Inflammation in Keloid Fibroblasts by Cold Atmospheric Plasma.","authors":"Razaul Haque, Sung Eun Chang, Ik Jun Moon","doi":"10.1159/000547259","DOIUrl":"10.1159/000547259","url":null,"abstract":"<p><strong>Introduction: </strong>Despite numerous therapeutic approaches, keloid treatment remains a challenge. Clinical studies have demonstrated the possible use of cold atmospheric plasma (CAP) to treat hypertrophic scars and keloids. This study investigated the effects and relative mechanisms of CAP treatment on primary keloid fibroblasts (PKF) in vitro.</p><p><strong>Methods: </strong>PKF cells from 10 patients with keloid and human dermal fibroblast (HDFa) cell line were cultured to compare CAP treatment effects. Cell proliferation, migration via scratch assay, and reactive oxygen species (ROS) levels were measured using standard assays, while cell apoptosis was quantified by flow cytometry. A quantitative reverse transcription polymerase chain reaction was performed to analyze the effect of CAP on gene regulation in fibrosis and inflammation. Finally, the mode of action of CAP was compared to H₂O₂ treatment.</p><p><strong>Results: </strong>CAP treatment in medium mode (CAP-mid), specifically for 30 and 60 s, significantly inhibited PKF proliferation and migration. No significant effects were seen in HDFa cells. Genetic analysis of pro-fibrotic components and inflammatory cytokines revealed that CAP-mid significantly reduced α-sma, periostin, h-col1, tgf-β, IL-6, and IL-31 expression in PKF cells, while it enhanced IL-10 expression. However, it had opposite effects on HDFa. Time-dependent analysis showed that CAP-mid at 60 and 30 s exerted the maximum effects on those molecules. Simultaneous analysis of CAP and H₂O₂ treatment on PKF cells demonstrated that CAP-mediated alterations in gene expression are primarily linked to enhanced ROS production in PKF cells.</p><p><strong>Conclusion: </strong>These findings suggest that CAP may mitigate keloid formation by modifying fibrotic and inflammatory profiles through ROS production and inhibition of cell proliferation.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"1-13"},"PeriodicalIF":3.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic Monitoring of in vitro Release Testing Using UV-Vis Spectrophotometry with Hydrocortisone Creams.","authors":"Kelsey Leach, Lola Sibaud, Bradley Towey","doi":"10.1159/000546534","DOIUrl":"https://doi.org/10.1159/000546534","url":null,"abstract":"<p><strong>Introduction: </strong>A new method for conducting in vitro release testing (IVRT) was developed by adapting Higuchi's square root approximation for use with UV-Vis spectrophotometry, and over the counter hydrocortisone formulations at 0.5% and 1.0% concentrations. This IVRT method was investigated for the required validation elements as specified by abbreviated new drug applications (ANDAs) and USP General Chapter <1724> for linearity and range, precision and reproducibility, and discrimination sensitivity, specificity, and selectivity.</p><p><strong>Methods: </strong>IVRT kinetic experiments were conducted using UV-Vis spectrophotometer, a quartz cuvette, with measurements collected every 15 s for 5 min, and methanol as the receptor solution. Six measurements of the 1% hydrocortisone formulation were conducted over 3 different days, for a total of 18 measurements. The 0.5% formulation was measured 6 times over 1 day. Release rates were obtained by plotting the slope of Abs242 vs. √t. HPLC was used to demonstrate specificity via an alternate analytical technique and to show membrane inertness.</p><p><strong>Results: </strong>The hydrocortisone cream formulations demonstrated specificity via HPLC compared to a USP traceable hydrocortisone reference standard. IVRT sensitivity and selectivity were demonstrated by the statistically different release rates (slopes) of the 0.5% vs. the 1% hydrocortisone formulations at 90% confidence interval (75-133.33%). Linearity throughout the duration of the assay was demonstrated through a r2 value of ≥0.97 for each experiment and for each formulation. All intra-run and inter-run precision calculations relating to the IVRT experiments had %CV values of ≤15%.</p><p><strong>Conclusion: </strong>IVRT experiments were conducted using UV-Vis spectrophotometry kinetic monitoring of 0.5% and 1% hydrocortisone formulations. This IVRT method was validated for specificity, selectivity, sensitivity, linearity and range, precision and reproducibility following the guidance for ANDAs and USP General Chapter <1724>, thus demonstrating the capability of UV-Vis spectrophotometry as a reliable way of discerning release rates of semisolid formulations. This novel approach can be conducted in a matter of minutes as opposed to hours, a vast improvement over conventional IVRT studies.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"1-7"},"PeriodicalIF":2.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Physical, Chemical, and Sympathetic Stimuli on Water-Immersion Finger Wrinkling.","authors":"Bruno D Martimiano, Maria C V Belli, Mariana de R Lai, Mariana M Morita, Fernando H Minagawa, Ana C C Espósito, Vitor C de Oliveira, Mariana P S Coelho, Hélio A Miot","doi":"10.1159/000546695","DOIUrl":"10.1159/000546695","url":null,"abstract":"<p><strong>Introduction: </strong>Stimulated skin wrinkling (SSW) reflects the integrity of the autonomic nervous system, eccrine sweat glands, and microcirculation. Specific stimuli, as water immersion or mechanical pressure usually elicit it. This study explored the influence of some physical, chemical, sympathetic stimuli, and circadian cycle on SSW.</p><p><strong>Methods: </strong>Time required to achieve grade III finger wrinkling was assessed in 24 healthy adults. SSW induced by room-temperature filtered water (RTFW) for comparison across temperature variations: warm water (40°C) and iced water; tonicity: Distilled water and hypertonic saline (36% NaCl); pH variations: acidic and alkaline solution; oily medium: soybean oil; or pressure: immersion under hydrostatic pressure (30 cm water column). To evaluate the influence of sympathetic activity, SSW was assessed under caffeine stimulation: 30 min after ingestion of 60 mg caffeine (Ristretto espresso); diurnal variation: testing at 10 a.m. versus 10 p.m.; or ischemic influence: under sub-systolic ischemia induced by an arm cuff. Additionally, 12 participants underwent 10 min fingertip exposures to EMLA and room-temperature vinegar for comparison.</p><p><strong>Results: </strong>The time to reach grade III SSW under RTFW varied widely across the sample (from 2.1 to 37.0 min). The acidic solution reduced the time to SSW compared to RTFW (mean: 6.4 vs. 13.9 min; p < 0.01), with a more pronounced effect observed with a warm (40°C) acidic solution (4.0 vs. 15.5 min; p < 0.01). Hot water, distilled water, and 30 cm hydrostatic pressure stimulation shortened the time to SSW compared to RTFW (p < 0.01). No SSW was observed after 30 min of immersion in the oily medium, iced water, alkaline solution, hypertonic solution, or following ischemia. A circadian influence on SSW was also observed, with morning measurements resulting in a faster response than at night (10.4 vs. 14.6 min; p < 0.01). SSW was also accelerated after coffee consumption (6.1 vs. 10.5 min; p < 0.01). After 10 min of stimulation with either EMLA or vinegar, 75% of participants reached grade III SSW (p = 1.00).</p><p><strong>Conclusion: </strong>SSW is influenced by multiple factors, including solution temperature, pH, osmolarity, hydrostatic pressure, caffeine ingestion, and circadian timing. Warm vinegar elicited a faster and more consistent SSW response compared to other tested conditions, highlighting its use in experimental settings. No difference in SSW was observed between room-temperature vinegar and EMLA after 10 min, supporting its potential for clinical application.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"121-126"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin Transcriptomic Analysis Reveals Altered Fatty Acid Metabolism Pathways in Mice Subjected to Sleep Fragmentation.","authors":"Da-Been Lee, Seung-Lim Yoo, June Seok Heo, Ja-Yun Lim, Sang Shin Pyo, Ji Sun Moon, Mi-Ran Lee, Jinkwan Kim, Suhng-Wook Kim, Dae-Wui Yoon","doi":"10.1159/000544736","DOIUrl":"10.1159/000544736","url":null,"abstract":"<p><strong>Introduction: </strong>Sleep fragmentation (SF) is a hallmark of sleep disorders and has been associated with systemic health issues, but its specific impact on skin health remains unclear. This study aimed to investigate whether SF impairs skin barrier function and identify the biological pathways involved in SF-induced skin damage.</p><p><strong>Methods: </strong>Twenty-four 6-week-old male BALB/c mice were divided into home cage control (HC) and SF groups. SF was induced using a commercially available SF chamber. Skin barrier function was assessed by measuring transepidermal water loss (TEWL) at 4 and 8 weeks. Epidermal thickness and dermal collagen density were also measured. Total RNA sequencing (RNA-Seq) and bioinformatics analysis were conducted to identify the affected pathways.</p><p><strong>Results: </strong>TEWL was significantly higher in the SF group than in the HC group at 8 weeks. Epidermal thickness and dermal collagen density were significantly lower in the SF group than in the HC group. In the SF group, 133 differentially expressed genes were identified, of which 14 were upregulated and 119 were downregulated. RNA-Seq and bioinformatics analysis revealed an altered fatty acid metabolism pathway in the skin of mice subjected to chronic SF. This was validated through quantitative real-time polymerase chain reaction.</p><p><strong>Conclusion: </strong>SF caused physiological and histological changes in the skin, altering the fatty acid metabolism pathway. The role of this pathway in SF-induced skin damage requires further exploration.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"21-34"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12136610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143450117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rigorously Simple Quantitative Model for Free Radical Behavior in Aerobic Biological Systems.","authors":"Leonhard Zastrow, Jürgen Lademann, Martina C Meinke, Silke B Lohan","doi":"10.1159/000542600","DOIUrl":"10.1159/000542600","url":null,"abstract":"<p><strong>Background: </strong>Human life is based on oxygen respiration and an enzymatic, free radical-dependent water chemistry, whose billions of parallel reactions take place at pH ∼7.4 and a temperature of 37°C, in accordance with the laws of chemistry. The cellular metabolic processes occur over time periods covered by the half-lives of reactive oxygen species (ROS) for °OH to over 10 s for lipid oxygen species (LOS), indicating that mixtures of free radicals form the basic components for these processes.</p><p><strong>Summary: </strong>The main source of radicals is the mitochondrial conversion of 1-5% oxygen into \"primary\" ROS and \"secondary\" LOS. Every endogenous and exogenous radical generation, triggered by \"natural background radiation,\" \"natural environment,\" or \"solar radiation\" leads to qualitatively similar mixtures of \"primary\" ROS and \"secondary\" LOS or RNS (reactive nitrogen species). A Multilevel Antioxidant Regulation, Repair and Protection System (MARRPS) keeps these radical mixtures in a steady state. Depending on the total number of free radicals, different areas of radical action are defined. The Free Radical Ground State (FRGS) with \"homeostasis\" and \"adaptive homeostasis,\" the Free Radical Threshold Value (FRTV), and Free Radical Pathological Conditions (FRPC). The quantitative ratio ROS > LOS comprehensively characterizes the \"'homeostasis\" and \"adaptive homeostasis\" area of the FRGS. The total number of free radicals cannot be measured directly in the \"homeostasis\" area. \"Adaptive homeostasis\" is achieved when excess radicals are stable produced beyond \"homeostasis\" of the FRGS. The quantity that remains controllable in this range is a maximum of ∼3.58 × 1012 radicals/mg, the value of the body constant FRTV. The sensitized MARRPS provides \"semi-stable homeostatic\" states characterized by dual stability with ROS > LOS and a stable total ROS/LOS and RNS count beyond the basal FRGS \"homeostasis.\" If the total number of all radicals exceeds the FRTV, where LOS > ROS, this initiates uncontrolled radical chain reactions. The partial failure of the MARRPS in the FRPC area leads to pathological processes which are the starting point for a hundred different diseases.</p><p><strong>Key messages: </strong>The universal body constant FRTV is the basis for all regular life processes. The design principle described by this simple model applies universally to all aerobic life.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"45-58"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emulsifier-Induced Changes to the Human Skin Barrier: Connection to Ceramide Profiles and Assessment as a Skin Lesion Model.","authors":"Moritz Reuter, Hans Schoenfelder, Annette Gaiser, Sebastian Volc, Dominique Lunter","doi":"10.1159/000545234","DOIUrl":"10.1159/000545234","url":null,"abstract":"<p><strong>Introduction: </strong>Emulsifiers are common excipients in dermal products stabilizing formulations such as creams and emulsions. But due to their potential for skin irritation, emulsifiers for pharmaceutical use should be tested regarding their tolerability before introducing them to the skin of patients. In this study, a systematic investigation with six oil in water-emulsifiers was performed on the forearms of 12 healthy human volunteers, six female, and six male.</p><p><strong>Methods: </strong>We analyzed the effects of pharmaceutical emulsifiers on the macroscopic skin health parameters measured as trans-epidermal water loss (TEWL) and skin hydration and measured the ceramide profile of the treated skin sites using liquid chromatography coupled to mass spectrometry in order to assess the skin tolerability of the investigated emulsifiers. In a second step, a Partial Least Squares Regression was employed to investigate relationships between changes in the ceramide profile to changes in the TEWL of skin treated with a nonionic as well as an anionic emulsifier.</p><p><strong>Results: </strong>Skin health measurements showed that the applied emulsifiers inflicted no significant changes compared to the water-treated sample, demonstrating a remarkable skin tolerability. The employed regression model showed a good fit as well as adequate prediction and identified ceramide species associated with impaired skin barrier function. Furthermore, it was found that the relationship between the ceramide profile and the skin barrier function in emulsifier-induced skin damage shows distinct similarities to the interplay of ceramides and skin barrier function in lesional skin linked to atopic dermatitis, hinting toward a common underlying mechanism and opening up possibilities to simulate disease-related changes to the skin for the development of skin damage models.</p><p><strong>Conclusion: </strong>In conclusion, these detailed investigations yield insight into possible mechanisms of emulsifier-induced skin damage and show its versatility in the investigation of pharmaceutical emulsifiers for formulation development as well as basic research.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"79-91"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Two Different Basic Skincare Regimens on Children and Adult Skin Microbiota: An Exploratory Randomized Controlled Crossover Trial.","authors":"Kathrin Hillmann, Tsenka Tomova-Simitchieva, Pauline Sophia Pinta, Zhile Xiong, Annette Moter, Varvara Kanti-Schmidt, Jan Kottner, Ulrike Blume-Peytavi","doi":"10.1159/000545433","DOIUrl":"10.1159/000545433","url":null,"abstract":"<p><strong>Introduction: </strong>Regular use of leave-on skincare products has positive effects on the skin barrier and appears to influence the skin microbiome in atopic dermatitis (AD). However, product-related effects on the skin microbiome are unclear. The aim of this study was to investigate to which extent the application of an emollient plus product differs from a petrolatum-based basic skincare product in terms of skin physiology, skin barrier, and skin microbiome.</p><p><strong>Methods: </strong>In an exploratory study regime, 50 subjects (children and adults) with mild AD were randomized to receive Bepanthen SensiDaily® (BSD) and Basiscreme DAC (DAC) for 3 months in a crossover design. Skin physiological measurements and skin microbiome swabs were taken on the forearm and lower leg before and after each 3-month application period of the respective product.</p><p><strong>Results: </strong>Improvement of skin physiological parameters after 3 months was observed in both topical leave-on product groups. Statistically significant differences were observed in baseline microbiomes between children and adults (p < 0.001) but not between the product groups.</p><p><strong>Conclusion: </strong>Regular application of leave-on products leads to better stratum corneum hydration after 3 months with better effects of BSD on the forearms of children and the lower legs of adults compared to DAC. The application of BSD and DAC did not lead to significant alterations in the overall composition of the skin microbiome. Although there were shifts in the frequency of certain microbial genera, these changes were not consistent between age groups and treatments.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"103-112"},"PeriodicalIF":3.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intake of Soymilk-Okara Powder for 8 Weeks Induced the Improvement of Skin Elasticity in Japanese Women.","authors":"Akihiro Maeta, Masahiro Katsukawa, Yaeko Hayase, Kyoko Takahashi","doi":"10.1159/000543802","DOIUrl":"10.1159/000543802","url":null,"abstract":"<p><strong>Introduction: </strong>Soymilk okara, a rich source of protein and dietary fiber, is a byproduct of soymilk manufacturing. We investigated whether soymilk-okara intake improved skin conditions in Japanese women with self-reported constipation.</p><p><strong>Methods: </strong>Thirty-seven Japanese women with self-reported constipation were included in this study. Two-thirds of the participants ingested 15 g of soymilk-okara powder daily for 8 weeks (okara group), whereas seasonal effects were evaluated in the remaining one-third (observation group). The participants' body composition and skin conditions (stratum corneum hydration, transepidermal water loss [TEWL], and gross elasticity [R2; epidermis and dermis]) of the malar and forehead were measured. Additionally, fecal concentrations of uremic toxins such as indole and p-cresol were analyzed.</p><p><strong>Results: </strong>Eight participants withdrew consent during the study period owing to COVID-19, etc., and the final data analysis was performed using data from participants in the okara (n = 19) and observation (n = 10) groups. No significant interactions among stratum corneum hydration, TEWL, or epidermal gross elasticity of the malar and forehead in the okara and observation groups were observed. In contrast, interactions of the dermis gross elasticity of the malar and forehead in both groups (p = 0.065 and 0.043, respectively) were observed. In the okara group, negative correlations between the changes in uremic toxins and the difference in the dermis gross elasticity of the forehead were observed.</p><p><strong>Conclusion: </strong>The intake of soymilk-okara powder improved skin elasticity, which may be a result of changes in the intestinal flora.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"35-44"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-211 Regulates Cutaneous Wound Healing through Inhibiting Inflammatory Reactions and Oxidative Stress by Binding SOX11.","authors":"Yun Chen, Xinyi Zhang, Fangfang Wu, Lixia Wang, Hongju Zuo, Hanbing Tian, Huan Chen","doi":"10.1159/000542697","DOIUrl":"10.1159/000542697","url":null,"abstract":"<p><strong>Introduction: </strong>Loss of skin integrity due to a wound or disease can lead to severe disability or even life threat. The highly expressed microRNAs in the skin are of great significance for skin development. The purpose of the investigation was to explore the effect and mechanism of miR-211 on inflammation, oxidative stress, and migration in keratinocytes.</p><p><strong>Methods: </strong>The HaCaT keratinocytes were treated with hydrogen peroxide (H2O2) to establish a wound-healing model. The expression of miR-211 was examined by quantitative real-time PCR. The cell function was reflected in proliferative ability, migration, apoptosis, and inflammation, which were evaluated using the Cell Counting Kit-8 (CCK-8) assay, transwell test, flow cytometry technique, and enzyme-linked immunosorbent assay (ELISA). The target of miR-211 was verified by luciferase luminescence measurements.</p><p><strong>Results: </strong>H2O2 inhibited HaCaT cell proliferation, migration, and promoted cell apoptosis, accompanied with the downregulation of miR-211. H2O2 led to inflammatory response and oxidative damage to HaCaT. miR-211 promoted proliferation and migration but improved cell apoptosis of HaCaT. The role of H2O2 on inflammatory response and oxidative stress was alleviated by miR-211. SRY-box transcription factor 11 (SOX11) was a targeted mediator of miR-211. SOX11 reversed the influence of miR-211 on cell proliferation, migration, apoptosis, inflammatory response, and oxidative stress.</p><p><strong>Conclusion: </strong>miR-211 regulated the proliferation, migration, apoptosis, inflammation, and oxidative stress of keratinocytes by mediating SOX11, thus participating in cutaneous wound healing.</p>","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":" ","pages":"10-20"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}