Molecular Nutrition & Food Research最新文献_第9页

Protective Effects of Propolis Supplementation on Aflatoxin B1-Induced Oxidative Stress, Antioxidant Status, Intestinal Barrier Damage, and Gut Microbiota in Rats 补充蜂胶对黄曲霉毒素 B1 诱导的大鼠氧化应激、抗氧化状态、肠道屏障损伤和肠道微生物群的保护作用

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-30 DOI: 10.1002/mnfr.70052

Sevtap Kabalı, Mehtap Ünlü Söğüt, Neslihan Öner, Ayça Kara

{"title":"Protective Effects of Propolis Supplementation on Aflatoxin B1-Induced Oxidative Stress, Antioxidant Status, Intestinal Barrier Damage, and Gut Microbiota in Rats","authors":"Sevtap Kabalı, Mehtap Ünlü Söğüt, Neslihan Öner, Ayça Kara","doi":"10.1002/mnfr.70052","DOIUrl":"https://doi.org/10.1002/mnfr.70052","url":null,"abstract":"Aflatoxin B1 (AFB1) is common in the diets of humans and animals and often leads to adverse health effects. Propolis, with its strong antioxidant activity, can reduce oxidative stress and modulate gut microbiota composition. However, the underlying mechanism by which propolis alleviates AFB1-induced intestinal barrier damage remains unclear. This study was designed to investigate the protective effects of oral propolis supplementation in AFB1-exposed rats. Thirty-two male Sprague-Dawley rats were divided into four groups: control, AFB1, propolis, and AFB1+propolis. After 4 weeks, serum oxidative stress markers were examined, and gut microbiota was analyzed by 16S rRNA sequencing. Intestinal sections were processed by Hematoxylin & Eosin staining, and the expression level of tight junction proteins was assessed by immunostaining. Propolis supplementation in AFB1-exposed rats tended to decrease oxidative stress, and it also restructured the gut microbiota by preventing a decrease in the relative abundances of <i>Lactobacillus</i>, <i>Roseburia</i>, and <i>Phascolarctobacterium</i>. Propolis restored intestinal permeability impaired by AFB1 by ameliorating intestinal morphological damage and increasing the expression levels of tight junction proteins. Propolis supplementation may contribute to the modulation of gut microbiota by alleviating oxidative stress and improving intestinal barrier damage in AFB1-exposed rats.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"31 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory Effects of Omega-3 Fatty Acids: Mechanistic Insights and Health Implications 欧米茄-3脂肪酸的免疫调节作用：机制见解和健康意义

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-30 DOI: 10.1002/mnfr.202400752

Mahmut Bodur, Birsen Yilmaz, Duygu Agagunduz, Yeşim Ozogul

{"title":"Immunomodulatory Effects of Omega-3 Fatty Acids: Mechanistic Insights and Health Implications","authors":"Mahmut Bodur, Birsen Yilmaz, Duygu Agagunduz, Yeşim Ozogul","doi":"10.1002/mnfr.202400752","DOIUrl":"https://doi.org/10.1002/mnfr.202400752","url":null,"abstract":"Omega-3 fatty acids play a significant role in immunomodulation, with nutrigenomic approaches highlighting their impact on gene expression related to immune responses. Research indicates that omega-3 fatty acids can modulate inflammatory pathways, potentially reducing chronic inflammation and enhancing immune function. This review discusses the intersection of nutrigenomics and nutriepigenomics, focusing on how omega-3 fatty acids influence gene expression, immune function, and overall health. The immune system is a complex network responsible for defending the body against pathogens and maintaining internal balance. Comprised of innate and adaptive immunity, the system involves various cells, tissues, and organs working together to combat infections and prevent diseases. Omega-3 polyunsaturated fatty acids (PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), play a significant role in modulating the immune system. These fatty acids influence immune cell function, membrane fluidity, and signaling processes, enhancing immune responses and reducing inflammation. Furthermore, EPA and DHA affect several signaling pathways, reducing the expression of proinflammatory cytokines and inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, a critical transcription factor in the inflammatory response. Additionally, they activate PPAR-γ, further diminishing inflammatory gene expression. As precursors to specialized proresolving lipid mediators, EPA and DHA help shift the lipid mediator profile from proinflammatory to antiinflammatory derivatives, thus aiding in the resolution of inflammation.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"37 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excess Fructose Intake Activates Hyperinsulinemia and Mitogenic MAPK Pathways in Association With Cellular Stress, Inflammation, and Apoptosis in the Pancreas of Rats 过量果糖摄入激活大鼠胰腺中与细胞应激、炎症和凋亡相关的高胰岛素血症和有丝分裂MAPK通路

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-28 DOI: 10.1002/mnfr.70048

Ceren Guney, Mehmet Eray Alcigir, Fatma Akar

{"title":"Excess Fructose Intake Activates Hyperinsulinemia and Mitogenic MAPK Pathways in Association With Cellular Stress, Inflammation, and Apoptosis in the Pancreas of Rats","authors":"Ceren Guney, Mehmet Eray Alcigir, Fatma Akar","doi":"10.1002/mnfr.70048","DOIUrl":"https://doi.org/10.1002/mnfr.70048","url":null,"abstract":"The increase in sugar consumption has been associated with current metabolic disease epidemics. This study aimed to investigate the pancreatic molecular mechanisms involved in cellular stress, inflammation, mitogenesis, and apoptosis in metabolic disease induced by high-fructose diet. Here, we used biochemical, histopathological, Western blot, and immunohistochemistry methods to determine the metabolic and pancreatic alterations in male <i>Wistar</i> rats fed 20% fructose in drinking water for 15 weeks. High-fructose consumption in rats increased the immunopositivity and protein expression of glucose transporter 2 (GLUT2) and insulin in the pancreatic tissue, in association with abdominal adiposity, hyperglycemia, and hypertriglyceridemia. The expressions of cellular stress markers, glucose-regulated protein-78 (GRP78) and PTEN-induced putative kinase 1 (PINK1), were increased in the pancreas. The levels of interleukin (IL)-6, nuclear factor kappa B (NFκB), tumor necrosis factor α (TNFα), and IL-1β and components of the Nod-like receptor protein 3 (NLRP3) inflammasome were elevated. Excess fructose intake stimulated the activation of mitogenic extracellular signal-regulated kinases 1/2 (ERK1/2), p38, and c-Jun N-terminal kinase (JNK)1 as well as the apoptotic p53 and Fas pathways in the pancreas of rats. There was also an increase in caspase-8 and caspase-3 cleavage. Our findings revealed that dietary high-fructose in the pancreas causes hyperinsulinemia due to the upregulation of GLUT2 together with cellular stress and inflammatory markers, thereby stimulates mitogenic mitogen-activated protein kinase (MAPK) and apoptosis pathways, resulting in a complex pathological situation.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"30 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydroxytyrosol Enhances Plasma Extracellular Vesicle Secretion, Modulates Their miRNAs Cargo, and Reduces LDL Oxidation in Humans: Postprandial and 1-Week Effects 羟酪醇增强血浆细胞外囊泡分泌，调节其mirna的载货，并降低人的LDL氧化：餐后和1周效应

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-28 DOI: 10.1002/mnfr.70039

Andrea del Saz-Lara, María-Carmen López de las Hazas, Joao Tomé-Carneiro, Maria-Ángeles Ávila-Gálvez, María Carmen Crespo, Carmen Mazarío-Gárgoles, Luis A. Chapado, Lidia Daimiel, Francesco Visioli, Victoria Martín-Santiago, Juan Carlos Espín, Alberto Dávalos

{"title":"Hydroxytyrosol Enhances Plasma Extracellular Vesicle Secretion, Modulates Their miRNAs Cargo, and Reduces LDL Oxidation in Humans: Postprandial and 1-Week Effects","authors":"Andrea del Saz-Lara, María-Carmen López de las Hazas, Joao Tomé-Carneiro, Maria-Ángeles Ávila-Gálvez, María Carmen Crespo, Carmen Mazarío-Gárgoles, Luis A. Chapado, Lidia Daimiel, Francesco Visioli, Victoria Martín-Santiago, Juan Carlos Espín, Alberto Dávalos","doi":"10.1002/mnfr.70039","DOIUrl":"https://doi.org/10.1002/mnfr.70039","url":null,"abstract":"Hydroxytyrosol (HT) consumption is associated with healthy outcomes. Specifically, its ability to modulate microRNA (miRNA) expression has been described. However, whether HT influences the secretion of extracellular vesicles (EVs) and their transported miRNAs remains unknown. Here, following HT administration, we explored the potential changes in EV secretion and in their miRNA cargo during postprandial state and after 1 week of daily intake. Twelve healthy participants received, first, a single 25 mg dose of HT (postprandial study) and then 25 mg HT daily, for one week (sustained study). Plasma and circulating plasma-isolated EVs were analyzed for HT and derived metabolites using UPLC-qTOF-MS and miRNAs by RNA-seq. HT induced the secretion of EVs 1-h post-ingestion and a 1-week daily administration of HT increased fasting EVs and reduced circulating oxidized LDL levels. RNA-seq analysis identified 55 modulated miRNAs (30 upregulated and 25 downregulated) related to response to oxygen level and gland development, following 1-week HT supplementation. This pilot study sheds light on the effects of HT consumption on EV secretion and modulation of exosomal miRNAs in humans, which has been scarcely studied to date. Further research is warranted to elucidate the molecular mechanisms underlying the observed effects.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"23 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Mechanism of Galangin on Hyperuricemic Nephropathy Based on Metabolomics and Network Pharmacology 基于代谢组学和网络药理学的高良姜治疗高尿酸血症肾病的机制研究

IF 4.5 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-27 DOI: 10.1002/mnfr.70029

Daermu Qumu, Mu Tian, Hengxi Li, Xiujuan Yang, Binhui Han, Lanting Wei, Bo Li, Mengxue Ma, Junjie He, Xiaoni Shao

{"title":"Study on the Mechanism of Galangin on Hyperuricemic Nephropathy Based on Metabolomics and Network Pharmacology","authors":"Daermu Qumu, Mu Tian, Hengxi Li, Xiujuan Yang, Binhui Han, Lanting Wei, Bo Li, Mengxue Ma, Junjie He, Xiaoni Shao","doi":"10.1002/mnfr.70029","DOIUrl":"10.1002/mnfr.70029","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Galangin (GAL), a flavonol found in <i>Alpinia officinarum</i> and propolis, is a promising functional food. This study investigated the therapeutic effects and mechanisms of GAL in mice with hyperuricemic nephropathy (HN) by focusing on renal metabolomics and network pharmacology. In this study, we conducted untargeted metabolomic analysis and network pharmacology prediction. Subsequently, a compound-reaction-enzyme-gene network was constructed based on the results of metabolomics and network pharmacology to elucidate potential connections. The results demonstrated that GAL can improve renal interstitial fibrosis and inflammatory infiltration and reduce serum levels of uric acid (UA), urea nitrogen (UREA), and creatinine (CREA). Metabolome analysis indicated that GAL affected thiamine, pyrimidine, nicotinate, nicotinamide, pyruvate, glyoxylate, and dicarboxylate metabolism. Network pharmacology and experimental results showed that GAL reduced the key target expression of the tumor protein P53 (TP53), tumor necrosis factor (TNF), signal transducer and activator of transcription 3 (STAT3), heat shock protein 90 alpha family class A member 1 (HSP90aa1), albumin (ALB), and caspase-3 (CASP3). GAL also downregulated the expression of Janus kinase 2 (JAK2), phospho-JAK2 (P-JAK2), and phospho-STAT3 (P-STAT3). Furthermore, a joint analysis of the metabolome and network pharmacology showed that GAL can reverse HN through amino acid metabolism, nucleotide metabolism, energy metabolism, and endocrine system pathways. GAL can alleviate HN effectively and might play synergistic therapeutic roles through regulating metabolic profiles and the JAK2/STAT3 signaling pathway.</p>\u0000 </section>\u0000 </div>","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"69 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apigenin Alleviates Intestinal Ischemia/Reperfusion Injury via Upregulating Nrf2-Mediated Tight Junction Integrity 芹菜素通过上调nrf2介导的紧密连接完整性减轻肠缺血再灌注损伤

IF 4.5 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-27 DOI: 10.1002/mnfr.70043

Bin Xu, Yan Zhuang, Ying Zhang, Suoning Liu, Rongjun Fan, Weiru Jiang

{"title":"Apigenin Alleviates Intestinal Ischemia/Reperfusion Injury via Upregulating Nrf2-Mediated Tight Junction Integrity","authors":"Bin Xu, Yan Zhuang, Ying Zhang, Suoning Liu, Rongjun Fan, Weiru Jiang","doi":"10.1002/mnfr.70043","DOIUrl":"10.1002/mnfr.70043","url":null,"abstract":"<div>\u0000 \u0000 <p>Epithelial barrier dysfunction, critically involved in intestinal ischemia/reperfusion (I/R) injury, is significantly regulated by Nrf2-mediated oxidative stress. Apigenin, a flavonoid commonly found in fruits and vegetables with diverse biological properties, has an unclear impact on intestinal I/R injury. We hypothesize that apigenin improves intestinal barrier dysfunction by activating Nrf2 signaling. Thirty rats were randomly divided into five groups to establish an I/R model using superior mesenteric artery occlusion. Hypoxia and re-oxygenation (H/R) model was developed utilizing Caco-2 and IEC-6 cells, which were exposed to hypoxic conditions followed by re-oxygenation. Apigenin protected against intestinal mucosal damage by suppressing inflammatory cytokines release (TNF-α, IL-1β, IL-6, MPO, <i>p</i> < 0.01), ameliorating oxidative stress (MDA, SOD, GSH, GSH-Px, <i>p</i> < 0.01), and improving barrier dysfunction (DAO and TEER, <i>p</i> < 0.01) both in vivo and in vitro, without causing significant changes in the corresponding normal controls (<i>p</i> > 0.05). Apigenin up-regulated the protein expression of Nrf2, HO-1, and tight junction (TJ) proteins (<i>p</i> < 0.01). Furthermore, the knockdown of Nrf2 significantly abrogated apigenin-enhanced the TJ expression. Apigenin pretreatment alleviates intestinal I/R-induced barrier damage through Nrf2 activation and TJ upregulation, offering new strategies for preventing or treating I/R-associated intestinal diseases.</p>\u0000 </div>","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"69 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenols: Potential Applications in Cancer Therapy 多酚：在癌症治疗中的潜在应用

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-27 DOI: 10.1002/mnfr.70011

María Oriol-Caballo, María Paz Moreno-Murciano, Rafael López-Blanch, José M. Estrela, Elena Obrador

{"title":"Polyphenols: Potential Applications in Cancer Therapy","authors":"María Oriol-Caballo, María Paz Moreno-Murciano, Rafael López-Blanch, José M. Estrela, Elena Obrador","doi":"10.1002/mnfr.70011","DOIUrl":"https://doi.org/10.1002/mnfr.70011","url":null,"abstract":"Polyphenols (PFs) are compounds found in fruits and vegetables, known for their health-related benefits, mainly including antioxidant, antiinflammatory, and anticancer properties. However, their efficacy is limited by poor bioavailability due to issues like low solubility, rapid metabolism, and extensive excretion. Thus, research has focused on improving delivery systems, such as, for example, nanoparticles, hydrogels, cocrystals, or conjugation with carrier molecules, which may protect PFs from degradation, improve solubility, and/or facilitate targeted delivery to cancer cells. PFs are promising in modulating cancer-related pathways like cell proliferation and death, or metastatic invasion, though their translation to patients is hindered by bioavailability and complex cancer mechanisms. This review analyzes factors that affect PF bioavailability, evidences of in vivo effects in animal models and their mechanisms, results from clinical trials, and strategies to enhance bioavailability. The idea that PFs need to directly interact with the cancer cell is challenged. Future research aims to optimize delivery systems, combine PFs with standard treatments, and explore their epigenetic effects, modulation of the tumor microenvironment, and interactions with gut microbiota. Advances in personalized medicine and structural modifications to improve stability and absorption could further enhance PF anticancer potential. Despite challenges, PFs remain a promising avenue for complementary oncotherapy solutions.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"61 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143713449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ProbML: A Machine Learning-Based Genome Classifier for Identifying Probiotic Organisms 基于机器学习的益生菌基因组分类器

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-26 DOI: 10.1002/mnfr.70025

Arjun Orkkatteri Krishnan, Lalit N. Mudgal, Vishesh Soni, Tulika Prakash

{"title":"ProbML: A Machine Learning-Based Genome Classifier for Identifying Probiotic Organisms","authors":"Arjun Orkkatteri Krishnan, Lalit N. Mudgal, Vishesh Soni, Tulika Prakash","doi":"10.1002/mnfr.70025","DOIUrl":"https://doi.org/10.1002/mnfr.70025","url":null,"abstract":"Probiotics are microorganisms that offer health benefits to the host. Traditional methods for identifying these organisms are time-consuming and resource-intensive. This study addresses the need for a more efficient and accurate approach to probiotic identification using machine learning (ML) techniques. The present study introduces ProbML, an ML-based approach for identifying probiotic organisms from whole genome sequences of prokaryotes. Among the five ML algorithms tested, XGBoost models demonstrated superior performance, achieving a maximum accuracy of 100% on learning data and 95.45% on an independent test dataset. This surpasses existing tools, which achieved 97.77% and 66.28% accuracy on the same datasets, respectively. The ProbML models were used to analyze 4728 genomes in the Unified Human Gastrointestinal Genome database, classifying 650 genomes as probiotics, with many previously unreported. A versatile GUI platform was also developed that employs ProbML models for probiotic classification or can be used to generate custom ML classifiers based on user-specific needs (https://github.com/sysbio-iitmandi/MLG_Dashboard). This study emphasizes the power of genomic data and advanced ML techniques in accelerating probiotic discovery.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"47 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of 1,3-Dioleoyl-2-palmitoylglycerol on Intestine Structural and Functional Development in Early Life 1,3-二油基-2-棕榈酰甘油对早期肠道结构和功能发育的影响

IF 4.5 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-24 DOI: 10.1002/mnfr.70051

Fang Wu, Yaqiong Liu, Ming Zhang, Xinlei Yuan, Yutong Jin, Yixuan Li, Ran Wang, Yanling Hao, Bing Fang

{"title":"Effects of 1,3-Dioleoyl-2-palmitoylglycerol on Intestine Structural and Functional Development in Early Life","authors":"Fang Wu, Yaqiong Liu, Ming Zhang, Xinlei Yuan, Yutong Jin, Yixuan Li, Ran Wang, Yanling Hao, Bing Fang","doi":"10.1002/mnfr.70051","DOIUrl":"10.1002/mnfr.70051","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>1,3-Dioleoyl-2-palmitoyl-glycerol (OPO) is a specific triglyceride in human breast milk, and it has been added to infant formula to mimic human breast milk fat. Existing studies only focused on its effects on fatty acid and calcium absorption, as well as the intestinal microbial composition; however, effects of OPO on the early-life development of intestine were still unclear. Our study explored the effects of OPO on intestinal epithelial structure and barrier construction in neonatal mice and the involvement of intestinal microorganisms. OPO supplementation significantly increased the number of intestinal stem cells, which in turn promoted villus and crypt, and promoted goblet cell and Paneth cell differentiation. OPO also promotes epithelial barrier integrity by increasing the expression of mucin 2, lysozyme 1, and tight junction proteins. Furthermore, the benefits of OPO were associated with the higher abundance of beneficial bacteria (<i>unclassified_f_Muribaculaceae</i>, <i>Akkermansia</i>, <i>Bifidobacterium</i>, and <i>Blautia</i>) and elevated butyrate levels. This study demonstrates the efficacy of OPO on intestinal health in neonatal mice beyond defecation, expands the understanding of the biological functions of OPO, and expands its application in intestinal health products targeting special populations, such as the elderly or individuals with intestinal fragility or injury.</p>\u0000 </section>\u0000 </div>","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"69 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143695389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gallic Acid Ameliorated Chronic DSS‐Induced Colitis Through Gut Microbiota Modulation, Intestinal Barrier Improvement, and Inflammation 没食子酸通过调节肠道菌群、改善肠道屏障和炎症改善慢性DSS诱导的结肠炎

IF 5.2 2区农林科学

Molecular Nutrition & Food Research Pub Date : 2025-03-24 DOI: 10.1002/mnfr.70024

Baoming Tian, Peng Ye, Xue Zhou, Jiangning Hu, Peiyi Wang, Ming Cai, Kai Yang, Peilong Sun, Xianguo Zou

{"title":"Gallic Acid Ameliorated Chronic DSS‐Induced Colitis Through Gut Microbiota Modulation, Intestinal Barrier Improvement, and Inflammation","authors":"Baoming Tian, Peng Ye, Xue Zhou, Jiangning Hu, Peiyi Wang, Ming Cai, Kai Yang, Peilong Sun, Xianguo Zou","doi":"10.1002/mnfr.70024","DOIUrl":"https://doi.org/10.1002/mnfr.70024","url":null,"abstract":"Scope: Gallic acid (GA) is recognized for its purported antiinflammatory properties. GA has been demonstrated to prevent and alleviate the symptoms of chronic colitis through the modulation of the gut microbiota, improvement of the intestinal barrier, and reduction of inflammation.Methods and results: In order to determine the mechanism by which GA exerts its protective effect against chronic colitis, mice were induced by dextran sulfate sodium (DSS). The reduction in the disease activity index by 25% and the decrease in colon tissue damage indicated that 36 days of GA intervention alleviated chronic DSS‐induced colitis symptoms. GA was observed to mitigate weight loss by 2.5% and the shortening of colon by 17.3%, and to diminish the expression of pivotal proteins within the TLR4/nuclear factor κB (NF‐κB) signaling cascades, consequently lowering the generation of inflammatory cytokines. Furthermore, GA effectively corrected the gut microbiota imbalance, increased the content of short‐chain fatty acids (SCFAs), which in turn suppressed inflammation, and enhanced tight junction protein expression, thereby strengthening the intestinal barrier. Conclusion: GA has the capacity to enhance the efficacy of chronic colitis through a multifaceted mechanism, influencing the gut microbiota, intestinal barrier function, and inflammatory processes. The findings highlight the potential of GA as a preventative strategy for chronic colitis.","PeriodicalId":212,"journal":{"name":"Molecular Nutrition & Food Research","volume":"11 1","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0