Effect of Lactobacillus plantarum Extracellular Vesicles on Lipid Metabolism in Mice Fed a High-Fat Diet.

This thesis aims to investigate the role of extracellular vesicles of Lactobacillus plantarum (LPEVs) in the regulation of high-fat diet (HFD)-induced disorders of lipid metabolism.We used ultracentrifugation to obtain LPEVs and performed preliminary characterization. After an obese mouse model was established via HFD feeding, LPEVs were used to treat mice with lipid metabolism disorders, and changes in the body weight, blood lipid levels, tissue levels, intestinal flora structure, differential metabolites, and regulation of key signaling pathway genes in these mice were observed. The LPEVs reduced body weight, alleviated aberrant lipid metabolism and inflammatory responses, improved the morphological structure of the ileum, enriched the intestinal flora, influenced the production of metabolites, and regulated genes associated with lipid metabolism in mice.Consequently, LPEVs intervention seems to regulate lipid metabolism disorders by regulating the gut microbiota and improving nutrient absorption, thereby optimizing lipid metabolism, and providing a theoretical basis for future research on the regulation of lipid metabolism by probiotic metabolites.