Progress in molecular biology and translational science最新文献

{"title":"Oral delivery of protein and peptide therapeutics.","authors":"Vivek P Chavda, Pankti C Balar","doi":"10.1016/bs.pmbts.2024.11.003","DOIUrl":"10.1016/bs.pmbts.2024.11.003","url":null,"abstract":"<p><p>Oral administration of proteins and peptides has gained significant attention recently due to its potential to transform therapeutic strategies, providing a non-invasive and patient-friendly method for delivering biopharmaceuticals. The primary hurdle in oral delivery stems from the harsh conditions of the gastrointestinal (GI) tract, characterized by acidic pH, enzymatic degradation, and limited permeability across the intestinal epithelium. Various innovative approaches have emerged to overcome these challenges, including nanoparticle-based delivery systems, mucoadhesive formulations, and chemical modifications of peptides aimed at improving stability and absorption rates. Nanoparticle-based delivery systems, such as liposomes, polymeric nanoparticles, and solid lipid nanoparticles, hold promise in protecting proteins and peptides from enzymatic degradation while enhancing their bioavailability. These nanoparticles can be tailored to target specific areas within the GI tract, extending drug release and enhancing therapeutic effectiveness. Mucoadhesive formulations utilize polymers like chitosan, alginate, and polyethylene glycol (PEG) derivatives to adhere to GI mucosal surfaces, prolonging residence time and facilitating drug absorption. Chemical modifications, such as PEGylation, glycosylation, and lipidation have been employed to enhance the stability and permeability of proteins and peptides in the GI tract. PEGylation, in particular, has been widely used to extend the circulation half-life and reduce the immunogenicity of therapeutic proteins. Advancements in nanotechnology, especially the development of smart nanocarriers capable of responsive drug release triggered by pH or enzymatic stimuli, show promise in further improving oral delivery of proteins and peptides. The integration of bioinformatics and computational modeling techniques has facilitated the design of novel drug delivery systems with optimized pharmacokinetic profiles. This chapter focuses on the advancements and challenges in the oral delivery of protein and peptide-based drugs, highlighting the innovative strategies being explored to enhance therapeutic outcomes.</p>","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"212 ","pages":"355-387"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An introduction to non-coding RNAs.","authors":"Charan Psvv, Adithya Joseph, Praveen Ebenezer, Vandana Sankar, Renuka Suravajhala, R Shyama Prasad Rao, Prashanth Suravajhala","doi":"10.1016/bs.pmbts.2025.01.006","DOIUrl":"10.1016/bs.pmbts.2025.01.006","url":null,"abstract":"<p><p>Non-coding RNAs (ncRNAs) are a group of RNAs that do not encode proteins but play key roles in diverse biological functions such as gene regulation, chromatin remodeling, and other cellular processes. Two major types are small ncRNAs (sncRNAs, with a size of ∼21-34 nucleotides) and long ncRNAs (lncRNAs, size of>200 nucleotides), and each has multiple subtypes. Notably, ncRNAs constitute over 90 % of the RNAs from the human genome. Exploring ncRNAs is important to understand the complexities of cellular regulations and for potential therapeutic applications as biomarkers or therapies in various diseases such as cancer. In this chapter, we provide a gist of ncRNAs, their role in regulation.</p>","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"214 ","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wearable biosensors: A comprehensive overview.","authors":"Kevin Y Wu, Meihua E Su, Yeonsu Kim, Louis Nguyen, Michael Marchand, Simon D Tran","doi":"10.1016/bs.pmbts.2025.05.011","DOIUrl":"https://doi.org/10.1016/bs.pmbts.2025.05.011","url":null,"abstract":"<p><p>Wearable biosensors are revolutionizing the landscape of modern healthcare by enabling continuous, non-invasive monitoring and real-time diagnostics across a myriad of medical applications. This chapter provides a comprehensive overview of wearable biosensors, beginning with an exploration of their fundamental components, including biological elements, transducers, and electronic interfaces. It categorizes these devices based on the types of biological matrices they utilize, such as tears and saliva, and the nanomaterials and transduction mechanisms that underpin their functionality. Highlighting state-of-the-art advancements, the chapter delves into specific applications in ophthalmology and oral health, showcasing innovative tear-based sensors for monitoring intraocular pressure and glucose levels, as well as saliva-based devices for detecting oral diseases and systemic biomarkers. Through detailed examples, such as multifunctional contact lenses and smart mouthguards, the chapter illustrates the potential of these technologies to transform disease detection, health monitoring, and personalized treatment strategies. Additionally, it addresses the current challenges in wearable biosensor development, including issues of sensor accuracy, durability, and user comfort, while outlining future directions for research and integration into everyday healthcare practices. This chapter aims to provide readers with a thorough understanding of wearable biosensors' current state, innovations, and future potential in enhancing health and wellness monitoring.</p>","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"215 ","pages":"101-154"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaccines reimagined: The peptide revolution in disease prevention.","authors":"Vivek P Chavda","doi":"10.1016/bs.pmbts.2024.11.002","DOIUrl":"10.1016/bs.pmbts.2024.11.002","url":null,"abstract":"<p><p>Peptide-based vaccines have emerged as a promising avenue in the realm of immunization strategies. This chapter provides an overview of the key aspects and advancements in peptide-based vaccine development. Peptides, as fragments of larger proteins, hold the potential to induce targeted immune responses while minimizing off-target effects. We discuss the principles of peptide selection, epitope identification, and delivery platforms, underscoring the importance of rational design to optimize immunogenicity. The integration of computational tools and advanced analytical methods has enabled the refinement of peptide vaccine candidates. Studies on infectious diseases, cancers, and new pathogens showcase the versatility and efficacy of peptide vaccines. As the field progresses, collaborative efforts between researchers, industry, and healthcare systems are essential to bridge the gap from laboratory research to clinical application. The future holds promise for peptide-based vaccines to contribute significantly to disease prevention and therapeutic intervention.</p>","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"212 ","pages":"329-354"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural prediction of potent non-coding RNAs.","authors":"Abhijit Beura, Gowrang Kasaba Manjunath, Tikam Chand Dakal, Abhishek Kumar","doi":"10.1016/bs.pmbts.2025.05.002","DOIUrl":"10.1016/bs.pmbts.2025.05.002","url":null,"abstract":"<p><p>Non-coding RNAs (ncRNAs) are vital regulatory molecules that play critical roles in gene expression, cellular signaling, and various biological processes. This review explores the diverse types of ncRNAs, including microRNAs, small interfering RNAs, and long non-coding RNAs, emphasizing their structural features and functional implications. Accurate structural predictions of ncRNAs are essential for understanding their interactions with other biomolecules and developing therapeutic strategies. Advances in computational methods and experimental techniques are enhancing our ability to predict RNA structures and elucidate their roles in health and disease. Despite significant progress, challenges remain in accurately modeling complex RNA structures and understanding the dynamic nature of RNA folding. Future research directions will focus on integrating multi-omics data, refining prediction algorithms, and addressing ethical considerations associated with ncRNA-based therapies. The potential applications of ncRNAs in drug discovery, biomarker identification, and synthetic biology underscore their importance in modern biomedical research.</p>","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"214 ","pages":"101-143"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissecting ncRNA pathways and omics integration.","authors":"Peter Chinedu Agu","doi":"10.1016/bs.pmbts.2025.01.001","DOIUrl":"10.1016/bs.pmbts.2025.01.001","url":null,"abstract":"<p><p>This chapter x-rays the pivotal roles of non-coding RNAs (ncRNAs) in gene regulation and cellular processes with emphasis on their diverse functions and mechanisms. The ncRNA types, which include miRNAs, siRNAs, and lncRNAs were highlighted stating their significance in gene regulation and disease. Then, the biogenesis and functional roles of these ncRNAs were explained with a focus on their transcription, processing, and mechanisms of action such as RNA interference and gene silencing. Furthermore, the chapter delves into the pathways of miRNAs, siRNAs, and lncRNAs to elucidate their regulatory roles in chromatin remodeling, transcription, and post-transcriptional processes. Omics technologies, including genomics, transcriptomics, proteomics, and epigenomics were piqued for their transformative impact on ncRNA research towards enabling comprehensive analysis and discovery of novel ncRNA functions. Consistently, the integration of multi-omics data, showcasing bioinformatics tools, and exemplified studies that reveal ncRNA networks and systems biology approaches were highlighted. These led to the suggestion that addressing technical and computational challenges, such as data quality, reproducibility, and integration is pivotal to future advancements in applications of lncRNA. Therefore, dissecting ncRNA Pathways and Omics Integration shows the potential applications of ncRNA research in personalized medicine and therapeutics which has culminated in its growing significance in biomedical research and its promise for innovative treatment strategies.</p>","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"214 ","pages":"81-100"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preface.","authors":"","doi":"10.1016/S1877-1173(25)00044-4","DOIUrl":"https://doi.org/10.1016/S1877-1173(25)00044-4","url":null,"abstract":"","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"212 ","pages":"xvii-xviii"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delivery of protein therapeutics and vaccines using their multivalent complexes with synthetic polyelectrolytes.","authors":"Alexander K Andrianov","doi":"10.1016/bs.pmbts.2024.04.005","DOIUrl":"10.1016/bs.pmbts.2024.04.005","url":null,"abstract":"<p><p>Clinical applications of protein and peptide-based therapeutics and vaccines are rapidly expanding. However, the development of promising new product candidates is often hindered by unfavorable pharmacokinetic profiles, which necessitate the implementation of drug delivery systems to improve protein stability and bioavailability. Non-covalent modification of proteins with synthetic polyelectrolytes, which relies on the strength of cooperative multivalent interactions, may offer potential advantages. In contrast to commonly employed covalent conjugation or microencapsulation methodologies, this technology offers dynamic protection of the protein thereby minimizing the loss of its biological activity, enabling \"mix-and-match\" formulation approaches, reducing manufacturing costs and simplifying regulatory processes. The range of potential life sciences applications ranges from immunopotentiation and vaccine delivery systems to long-circulating stealth biotherapeutics. This review analyses current technology in the context of intended clinical indications and discusses various synthetic and formulation approaches leading to supramolecular complexation. It evaluates dynamic interactions of complexes with constituents of physiological compartments and attempts to identify critical factors that can affect future advancement of this paradigm-shifting protein delivery technology.</p>","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"212 ","pages":"235-259"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-active peptides for anticancer therapies.","authors":"Charles H Chen","doi":"10.1016/bs.pmbts.2024.10.005","DOIUrl":"10.1016/bs.pmbts.2024.10.005","url":null,"abstract":"<p><p>Membrane-active peptides are found in many living organisms and play a critical role in their immune systems by combating various infectious diseases. These host defense peptides employ multiple mechanisms against different microorganisms and possess unique functions, such as anti-inflammatory and immunomodulatory effects, often working in synergy with other antimicrobial agents. Despite extensive research over the past few decades and the identification of thousands of sequences, only a few have been successfully applied in clinical settings and received approval from the U.S. Food and Drug Administration. In this chapter, we explore all peptide therapeutics that have reached the market, as well as candidates in preclinical and clinical trials, to understand their success and potential applications in cancer therapy. Our findings indicate that at least four membrane-active peptide drugs have progressed to preclinical or clinical phases, dmonstrating promising results for cancer treatment. We summarize our insights in this chapter, highlighting the potential of membrane-active anticancer peptide therapeutics and their applications as targeting ligands in various biomedical fields.</p>","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"212 ","pages":"67-116"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preface.","authors":"","doi":"10.1016/S1877-1173(25)00119-X","DOIUrl":"10.1016/S1877-1173(25)00119-X","url":null,"abstract":"","PeriodicalId":21157,"journal":{"name":"Progress in molecular biology and translational science","volume":"214 ","pages":"xv-xvi"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}