Protoplasma最新文献

{"title":"Ontogenetic differences in sun and shade galls of Clinodiplosis profusa on Eugenia uniflora leaves and the cytological antioxidant mechanisms in gall cells.","authors":"Rayssa Rosa Marquesine, Yve Canaveze, Bruno Garcia Ferreira","doi":"10.1007/s00709-024-01973-8","DOIUrl":"10.1007/s00709-024-01973-8","url":null,"abstract":"<p><p>The gall-host Eugenia uniflora (Myrtaceae) is adaptable to different light conditions, enabling leaf production and survival in both sun and shade. Leaves of E. uniflora in shaded environments have more mesophyll layers, and galls of Clinodiplosis profusa (Cecidomyiidae) are larger and wider. Based on these previous observations, this study investigated the morphogenesis of galls induced by C. profusa on leaves of E. uniflora in different light conditions, revealing if the galls have a potential for acclimation, as observed with leaves. For this purpose, we compared the anatomical, histometric, and histochemical development of leaves and galls at different stages of development in sun and shade environments. Additionally, we analyzed the cytological features of the tissues composing the mature gall walls. Cells of shade galls expanded more toward the end of the developmental phase, which may explain the larger volume found for shade galls in a previous study. However, during the mature phase, these galls showed no significant differences in tissue thickness and final cell elongation in the contrasting light conditions. In the ultrastructural analyses, mature galls showed a gradient distinguishing the outer and inner parenchyma cells. The inner parenchyma had nutritive cells, with dense cytoplasm and abundant organelles. A higher accumulation of starch grains in nutritive cells, with evidence of hydrolysis of starch grains detected in the innermost layers leads to the accumulation of reducing sugars, which, with the presence of plastoglobules and protein bodies, are important mechanisms of oxidative stress dissipation in the cells in contact with the gall inducer.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"15-34"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution to the knowledge of the anatomy, histochemistry, and phenolic composition of leaf and stems of bilberry (Vaccinium myrtillus L.) cultivated in Tucumán, Argentina.","authors":"María Inés Mercado, Emilio Lizarraga, Roxana Alejandra Rubis, Susana Beatriz Genta, Natalia Cecilia Habib","doi":"10.1007/s00709-024-01981-8","DOIUrl":"10.1007/s00709-024-01981-8","url":null,"abstract":"<p><p>The Vaccinium genus, with over 200 species, is prized for its fruits and traditional medicinal uses. Introduced to South America in the 1980s, it has become a significant crop, particularly in Tucumán, Argentina. Southern highbush blueberries are the most cultivated. Recent research suggests that the leaves and stems of these species contain higher levels of beneficial compounds compared to fruits. This study explores the potential of V. myrtillus L. leaves and stems, typically discarded as agricultural waste, as sources of bioactive compounds. It provides the first detailed analysis of their anatomy and chemical composition, revealing high levels of phenolic compounds with antioxidant properties. Leaf extracts show stronger antioxidant activity compared to stems. Toxicity tests on Artemia salina indicate their safety for further exploration. These findings suggest that V. myrtillus L. waste by-products could be valuable as sources of bioactive compounds, promoting their application in pharmaceuticals, food, or cosmetics industries.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"117-131"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chloroplasts with clefts and holes: a reassessment of the chloroplast shape using 3D FE-SEM cellular reconstruction of two species of Chlamydomonas.","authors":"Naoki Sato, Mayuko Sato, Mayumi Wakazaki, Takashi Moriyama, Takashi Hirashima, Kiminori Toyooka","doi":"10.1007/s00709-024-01990-7","DOIUrl":"10.1007/s00709-024-01990-7","url":null,"abstract":"<p><p>Chloroplasts are usually considered spheroid organelles, but this is not the only shape of chloroplasts. The chloroplast of Chlamydomonas has been typically described as cup-shaped. However, in old studies, it was also modeled as a complex shape with \"perforations\" or windows. Here, we reconstructed the cellular architecture of Chlamydomonas reinhardtii and C. applanata using an array tomography system installed on a field emission scanning electron microscope. C. reinhardtii chloroplasts resembled a baseball glove or a cup without a side, featuring numerous large and small holes that may facilitate the transport of metabolites and proteins produced in the Golgi apparatus fitted in the holes. In a lipid-accumulating, high-light condition, the chloroplast volume increased by filling the side cleft with an entire wall. Many accumulated large lipid droplets were accommodated within the chloroplast holes, which could have been considered as \"chloroplast lipid droplets.\" Mitochondrial meshworks surrounded the chloroplast. C. applanata chloroplasts appeared like a folded starfish or a cup with many side clefts and a few holes. There was a single mitochondrion or two that branched in a complex form. Tight contacts of various organelles were also found in C. applanata. These reconstructions illustrate the complexity of chloroplast shape, which necessitates a revised understanding of the localization of lipid droplets and the evolution of chloroplasts: The prevailing image of the spheroid chloroplasts that reminds us of the similarity between chloroplasts and cyanobacteria is no longer tenable.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"207-218"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11698768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of drought acclimation on sugar metabolism in millet.","authors":"Joseph N Amoah, Monica Ode Adu-Gyamfi","doi":"10.1007/s00709-024-01976-5","DOIUrl":"10.1007/s00709-024-01976-5","url":null,"abstract":"<p><p>Drought stress triggers sugar accumulation in plants, providing energy and aiding in protection against oxidative damage. Plant hardening under mild stress conditions has been shown to enhance plant resistance to severe stress conditions. While sugar accumulation and metabolism under drought stress have been well-documented in crop plants, the effect of drought acclimation treatment on sugar accumulation and metabolism has not yet been explored. In this study, we investigated the impact of drought stress acclimation on sugar accumulation and metabolism in the leaves and root tissues of two commonly cultivated foxtail millet (Setaria italica L.) genotypes, 'PI 689680' and 'PI 662292'. Quantification of total sugars (soluble sugar, fructose, glucose, and sucrose), their related enzymes (SPS, SuSy, NI, and AI), and the regulation of their related transcripts (SiSPS1, SiSuSy1, SiSWEET6, SiA-INV, and SiC-INV) revealed that drought-acclimated (DA) plants exhibited levels of these indicators comparable to those of control plants. However, under subsequent drought stress conditions, both the leaves and roots of non-acclimated plants accumulated higher levels of total sugars, displayed increased activity of sugar metabolism enzymes, and showed elevated expression of sugar metabolism-related transcripts compared to drought-acclimated plants. Thus, acclimation-induced restriction of sugar accumulation, transport, and metabolism could be one of the metabolic processes contributing to enhanced drought tolerance in millet. This study advocates for the use of acclimation as an effective strategy to mitigate the negative impacts of drought-induced metabolic disturbances in millet, thereby enhancing global food security and promoting sustainable agricultural systems.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"35-49"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11698784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscopic structure and immunolabeling of extremely overlapped scales in some scincid, anguid, and pygopod lizards.","authors":"Lorenzo Alibardi","doi":"10.1007/s00709-024-01982-7","DOIUrl":"10.1007/s00709-024-01982-7","url":null,"abstract":"<p><p>Skink, anguid, and pygopod lizards possess an extremely flat skin, imparting a compact and solid body and shining surface that facilitates their slider and/or fossorial movements. The present morphological study, conducted using immunohistochemistry and electron microscopy, has analyzed the microscopical morphology of extremely overlapped scales in different lizards, including species with limb reduction (scincids such as Lerista bougainvilli, Scincella lateralis, Lampropholis delicata) or legless (pygopods such as Lialis burtonis and Delma molleri and the anguid Anguis fragilis). The outer surface of the epidermis shows different micro-structures of the Oberhautchen layer containing corneous beta-proteins (CBPs) with variable immunoreactivity for these proteins. The beta-layer is relatively thick in most of these species, probably in relation to the resistance against strong mechanical forces acting on scales during the movements on harsh substrates. The scincid and anguid lizards also possess and regenerate osteoderms that reinforce scales flatness and mechanical resistance during the serpentiform or fossorial movements of these reptiles. Osteoderms are absent in pygopods. Roundish cells with a granular content are detected in the deep hinge region of scales in Lerista and Lampropholis skinks. Whether these cells may secrete substances that facilitate scale anti-friction and also determine shining of the skin surface remains to be shown.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"99-115"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142111336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.).","authors":"Lijiang Hou, Dongzhi Zhang, Qiufang Wu, Xinqiang Gao, Junwei Wang","doi":"10.1007/s00709-024-01983-6","DOIUrl":"10.1007/s00709-024-01983-6","url":null,"abstract":"<p><p>Purple acid phosphatases (PAPs) play a vital role in plant phosphorus nutrition, serving as a crucial family of metallo-phosphoesterase enzymes. This research aimed to identify the PAP genes from the A/B/D genomes of Triticum aestivum to elucidate evolutionary mechanisms of the gene family in plants and provide genomic information for subsequent research on phosphorous-use efficiency in wheat crops. In total, 105 PAP genes (TaPAPs) were identified from the A/B/D genomes by using the Arabidopsis thaliana and Oryza sativa PAP protein sequences as queries for BLASTP against the wheat protein database. The TaPAPs were grouped into six subfamilies, Ia (17), Ib (26), IIa (11), IIb (30), IIIa (12), and IIIb (9), based on their similarities in the structure of genes and the presence of conserved protein motifs. A majority of TaPAPs were derived from tandemly (20) or segmentally (87) duplicated, with the homoeologous chromosomes 5A/B/D harboring the most duplicated PAP genes. Further analysis indicated that TaPAPs were responsible for the modulation of seed, root, and leaf development and hormone synthesis and signaling, as well as plant responses to abiotic stresses, including low temperatures, drought, and anaerobic conditions. Nine TaPAPs (TaPAP9-4A/4B/4D, TaPAP24-6A/6B/6D, and TaPAP28-7A/7B/7D) were constitutively expressed in diverse tissues such as root, shoot, leaf, spike, and seed, while the remaining genes exhibited tissue-specific expression patterns. Concerning the response to phosphate (Pi) deprivation, 57 TaPAPs were highly expressed in roots under Pi stress, including TaPAP31-4A, 4B, and 4D homeologs from the subfamily IIIb. A TaPAP31-4A transgene in A. thaliana promoted plant growth and development while increasing plant resistance to Pi-deficiency stress by enhancing the secretion of phosphatase. These discoveries provide a scientific foundation for comprehending the role of TaPAPs, offering valuable insights for identifying additional candidate genes and fostering the development of new wheat varieties with enhanced tolerance to low phosphorus conditions.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"73-86"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142111335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aerial and terrestrial root habits influence the composition of the cell walls of Vanilla phaeantha (Orchidaceae).","authors":"Jéssica Ferreira de Lima, Denis Coelho de Oliveira, Vinícius Coelho Kuster, Ana Silvia Franco Pinheiro Moreira","doi":"10.1007/s00709-024-01980-9","DOIUrl":"10.1007/s00709-024-01980-9","url":null,"abstract":"<p><p>In response to the restrictions imposed by their epiphytic habit, orchids have developed structural traits that allow greater efficiency in water uptake and use, such as a complex adventitious root system with velamen. The composition of cell wall of this specialized epidermis can be altered according to the substrate to which it is fixed, influencing wall permeability, absorption, and storage of water in roots. The current study aimed to evaluate the cell wall composition of adventitious roots of Vanilla phaeantha (Orchidaceae) that grow attached to the phorophyte, fixed in the soil, or hung free. Immunocytochemical analyses were used to determine the protein, hemicellulose, and pectin composition of the cell walls of aerial and terrestrial roots. We observed that pectins are present in the different tissues of the aerial roots, while in the terrestrial roots, they are concentrated in the cortical parenchyma. The deposition of xyloglucans, extensins, and arabinogalactans was greater in the epidermis of the free side of the roots attached to the phorophyte. The strong labeling of pectins in aerial roots may be related to the influx of water and nutrients, which are generally scarce in this environment. The arrangement of hemicelluloses and proteins with the pectins may be associated with increased cell rigidity and sustainability, a feature of interest for the aerial roots. In summary, the habit of roots can interfere with the non-cellulosic composition of the cell walls of V. phaeantha, possibly related to changes in cell functionality.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"87-98"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142111334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of Capnodium alfenasii with extrafloral nectaries of Azadirachta indica.","authors":"Naasoom Luiz Santos Mesquita, Carlos André Espolador Leitão, Poliana Prates de Souza Soares, Quelmo Silva de Novaes, Maruzanete Pereira de Melo, José Luiz Bezerra, Armínio Santos","doi":"10.1007/s00709-024-01977-4","DOIUrl":"10.1007/s00709-024-01977-4","url":null,"abstract":"<p><p>Sooty moulds are saprophytic epiphytic fungi that grow mostly on insect secretions, but they can also be associated with plant secretions. In this study, we aimed to describe de interaction of Capnodium alfenasii sooty mould with the extrafloral shoot nectaries of Azadirachta indica. Anatomical and histochemical studies were carried out on serial sections of extrafloral shoot nectaries of A. indica without and with C. alfenasii infestation. The total soluble sugar content of the secreted nectar was determined, and the conidial germination of the fungus in distilled water and in dextrose and nectar solutions was evaluated. The shoot nectaries of A. indica are elongated structures that occur in pairs near the base of the petiole. The exuded nectar contains an average of 534.8 µg of total soluble sugars per µL of nectar and provides ideal conditions for conidial germination and fungal growth. C. alfenasii hyphae grow on the nectary, penetrate through breaks in the cuticle, travel under the cuticle and penetrate the secretory tissue by inter- and intracellular routes. The present report is the first to describe the interaction of C. alfenasii with the A. indica nectary, including the penetration of hyphae into nectariferous tissues and the plant defence mechanisms.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"51-59"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated insights into the cytological, histochemical, and cell wall composition features of Espinosa nothofagi (Hymenoptera) gall tissues: implications for functionality.","authors":"Lubia María Guedes, Narciso Aguilera, Vinícius Coelho Kuster, Renê Gonçalves da Silva Carneiro, Denis Coelho de Oliveira","doi":"10.1007/s00709-024-01985-4","DOIUrl":"10.1007/s00709-024-01985-4","url":null,"abstract":"<p><p>Many insect-induced galls are considered complex structures due to their tissue compartmentalization and multiple roles performed by them. The current study investigates the complex interaction between Nothofagus obliqua host plant and the hymenopteran gall-inducer Espinosa nothofagi, focusing on cell wall properties and cytological features. The E. nothofagi galls present an inner cortex with nutritive and storage tissues, as well as outer cortex with epidermis, chlorenchyma, and water-storing parenchyma. The water-storing parenchyma cells are rich in pectins, heteromannans, and xyloglucans in their walls, and have large vacuoles. Homogalacturonans contribute to water retention, and periplasmic spaces function as additional water reservoirs. Nutritive storage cell walls support nutrient storage, with plasmodesmata facilitating nutrient mobilization crucial for larval nutrition. Their primary and sometimes thick secondary cell walls support structural integrity and act as a carbon reserve. The absent labeling of non-cellulosic epitopes indicates a predominantly cellulosic nature in nutritive cell walls, facilitating larval access to lipid, protein, and reducing sugar-rich contents. The nutritive tissue, with functional chloroplasts and high metabolism-related organelles, displays signs of self-sufficiency, emphasizing its role in larval nutrition and cellular maintenance. Overall, the intricate cell wall composition in E. nothofagi galls showcases adaptations for water storage, nutrient mobilization, and larval nutrition, contributing significantly to our understanding of plant-insect interactions.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"149-165"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smoke-water treatment of seeds, an ancient technique for increasing seed vigor.","authors":"Nidhi Pandey, Sandeep Nalla, Abhinav Dayal, Prashant Rai, Vaidurya Pratap Sahi","doi":"10.1007/s00709-024-01975-6","DOIUrl":"10.1007/s00709-024-01975-6","url":null,"abstract":"<p><p>Germination is an essential phenomenon in the life cycle of plants, and a variety of external and internal factors influence it. Fire and the produced smoke have been vital environmental stimulants for the germination of seeds in many plant species, like Leucospermum cordifolium and Serruria florida. These plants do not germinate at all if fire and smoke are not present. This phenomenon of germination in plant species has existed in the ecosystem since ancient times. Various studies to study the response of seeds to smoke and its extracts have been undertaken for stimulation of germination by burning various plant materials and bubbling the smoke produced through water. The application of plant-derived smoke and smoke water is well known for promoting germination, breaking dormancy, and checking abiotic stress. This significantly indicates that plant-derived smoke contains some bioactive metabolites responsible for the physiological metabolism of seed germination and is involved in enhancing seed vigor. The present review deals with the ancient use of smoke and smoke extracts for seed priming, the cost-efficient method of its preparation, the mode of action of karrikins relating to its perception by plants, and its significant effects on various crops, including its ability to check biotic and abiotic stresses.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"3-13"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141996281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}