Anti-microtubular activity of total alkaloids and aqueous extract of Detarium microcarpum a medicinal plant harvested in Mali.

IF 2.5 3区生物学 Q3 CELL BIOLOGY

Protoplasma Pub Date : 2024-11-07 DOI:10.1007/s00709-024-02003-3

Niaboula Dembele, Aimé Ainin Somboro, Nah Traore, Mamadou Badiaga, Salimatou Cisse, Mody Cisse, Peter Nick

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Abstract

Detarium microcarpum, is a species confined to drier regions of west and central Africa used to treat various diseases including cancer. Phytochemical screening revealed the presence of secondary metabolites (alkaloids) The aim of this work is to study the effect of total aqueous extracts and alkaloid fractions from D. microcarpum leaves, bark and roots on Nicotiana tabacum L. cv. 'Bright Yellow 2' (BY-2) tobacco cell line GFP-TuA3 expressing a N-terminal fusion of GFP. The plant was harvested in two different regions of Mali with a contrasting climate. The effects of the extracts on the microtubules was followed by spinning disc confocal microscopy. We showed that the anti-microtubular effect of the extracts is dose-dependent, depends of the sampling site and the part of the plant used. Total alkaloids extracted of D. microcarpum bark have more effect on microtubules than leaf and root. The bioactivity-guided fractionation should be used to screen out the biologically active compounds of the total alkaloid extracts of the bark of D. microcarpum.

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马里药用植物 Detarium microcarpum 总生物碱和水提取物的抗微管活性。

Detarium microcarpum 是非洲西部和中部较干旱地区的一个物种，用于治疗包括癌症在内的各种疾病。这项工作的目的是研究从 D. microcarpum 的叶、皮和根中提取的总水提取物和生物碱馏分对表达 N 端融合 GFP 的烟草细胞系 GFP-TuA3 的影响。该植物在马里两个气候截然不同的地区收获。我们用旋转圆盘共聚焦显微镜观察了提取物对微管的影响。结果表明，提取物的抗微管作用与剂量有关，取决于取样地点和使用的植物部分。与叶和根相比，D. microcarpum 树皮提取的总生物碱对微管的影响更大。应采用生物活性指导分馏法来筛选出小果桉树树皮总生物碱提取物中具有生物活性的化合物。

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来源期刊

Protoplasma 生物-细胞生物学

CiteScore

6.60

自引率

6.90%

发文量

审稿时长

4-8 weeks

期刊介绍： Protoplasma publishes original papers, short communications and review articles which are of interest to cell biology in all its scientific and applied aspects. We seek contributions dealing with plants and animals but also prokaryotes, protists and fungi, from the following fields: cell biology of both single and multicellular organisms molecular cytology the cell cycle membrane biology including biogenesis, dynamics, energetics and electrophysiology inter- and intracellular transport the cytoskeleton organelles experimental and quantitative ultrastructure cyto- and histochemistry Further, conceptual contributions such as new models or discoveries at the cutting edge of cell biology research will be published under the headings "New Ideas in Cell Biology".