Protoplasma最新文献_第10页

Endosperm and amyloplast development in waxy wheat cultivars. 蜡质小麦栽培品种的胚乳和淀粉体发育。

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-09-01 DOI: 10.1007/s00709-023-01889-9

Juan Liu, Yuangang Zhu, Kaibo Yang, Jian Song, Tisen Xu, Zhongmin Dai

{"title":"Endosperm and amyloplast development in waxy wheat cultivars.","authors":"Juan Liu, Yuangang Zhu, Kaibo Yang, Jian Song, Tisen Xu, Zhongmin Dai","doi":"10.1007/s00709-023-01889-9","DOIUrl":"10.1007/s00709-023-01889-9","url":null,"abstract":"The endosperm is an essential part of wheat grains, and the accumulation of amyloplasts in endosperm determines the quality of wheat. Because waxy wheat has a special starch quality, there is a need to understand differences in endosperm and starch morphologies among waxy wheat cultivars. This study investigated differences in the endosperm and amyloplasts of two near-isogenic lines (Shimai19-P and Shimai19-N) and the wheat cultivar Shimai19 during various growth stages using light microscopy and scanning electron microscopy. At 8 days after pollination (DAP), with endosperm development, the amyloplast distributions in the different endosperm regions of the three wheat varieties were in the following order: center of ventral endosperm > subaleurone of ventral endosperm > center of dorsal endosperm > modified aleurone > subaleurone of dorsal endosperm. At 16 DAP, small amyloplasts appeared in the endosperm cells in all three wheat cultivars; subsequently, endosperm cell development until maturity was more rapid in Shimai19-N than in the other varieties. This study revealed variations in amyloplast accumulation among endosperm regions and waxy wheat varieties during wheat grain development, which improved the understanding of nutrient accumulation and nutrient transfer of wheat grains.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10185248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rediscovering Chara as a model organism for molecular and evo-devo studies. 重新发现Chara作为分子和进化研究的模式生物。

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-10-25 DOI: 10.1007/s00709-023-01900-3

Katarina Kurtović, Vojtěch Schmidt, Martina Nehasilová, Stanislav Vosolsobě, Jan Petrášek

{"title":"Rediscovering Chara as a model organism for molecular and evo-devo studies.","authors":"Katarina Kurtović, Vojtěch Schmidt, Martina Nehasilová, Stanislav Vosolsobě, Jan Petrášek","doi":"10.1007/s00709-023-01900-3","DOIUrl":"10.1007/s00709-023-01900-3","url":null,"abstract":"Chara has been used as a model for decades in the field of plant physiology, enabling the investigation of fundamental physiological processes. In electrophysiological studies, Chara has been utilized thanks to its large internodal cells that can be easily manipulated. Additionally, Chara played a pioneering role in elucidating the presence and function of the cytoskeleton in cytoplasmic streaming, predating similar findings in terrestrial plants. Its representation considerably declined following the establishment and routine application of genetic transformation techniques in Arabidopsis. Nevertheless, the recent surge in evo-devo studies can be attributed to the whole genome sequencing of the Chara braunii, which has shed light on ancestral traits prevalent in land plants. Surprisingly, the Chara braunii genome encompasses numerous genes that were previously regarded as exclusive to land plants, suggesting their acquisition prior to the colonization of terrestrial habitats. This review summarizes the established methods used to study Chara, while incorporating recent molecular data, to showcase its renewed importance as a model organism in advancing plant evolutionary developmental biology.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50162658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaf secretory structures in Rosa lucieae (Rosaceae): two times of secretion-two ecological functions? 蔷薇科植物蔷薇的叶片分泌结构：两种分泌时间-两种生态功能？

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-09-12 DOI: 10.1007/s00709-023-01892-0

Valdnéa Casagrande Dalvi, Maycon de Sousa Silva, Alex Batista Moreira Rios, Ítalo Antônio Cotta Coutinho

{"title":"Leaf secretory structures in Rosa lucieae (Rosaceae): two times of secretion-two ecological functions?","authors":"Valdnéa Casagrande Dalvi, Maycon de Sousa Silva, Alex Batista Moreira Rios, Ítalo Antônio Cotta Coutinho","doi":"10.1007/s00709-023-01892-0","DOIUrl":"10.1007/s00709-023-01892-0","url":null,"abstract":"Secretory trichomes and colleters are two of the secretory structures whose exudates may cover the body of the plant. Such secretions comprise resins or mucilages which are associated with an array of ecological roles. In Rosaceae, secretory trichomes have been reported for the leaves while colleters associated with leaf teeth. Our study aimed to identify the secretory structures of Rosa lucieae and understand the ecological role played by these glands as interpreted by morphoanatomical and histochemical studies. Samples from developing and fully mature leaves were collected, fixed, and processed according to usual techniques for light and scanning electron microscopy. In R. lucieae, colleters are restricted to the leaf and stipular margins and are associated with the teeth. They present a parenchymatous axis surrounded by a secretory palisade epidermis and usually fall off after the secretory activity is finished. Different from colleters, secretory trichomes are persistent. They present a multicellular secretory head and stalk. They are found at the base of the leaflet, petiolule, rachis, and petiole and occasionally on the stipular and leaf margins. The colleters predominantly secrete mucilages while the secretory trichomes secrete lipids and terpenes, both via cuticle rupture. The secretory activity of colleters is predominant in the leaf primordia, holding leaflets together and protecting meristems and leaves from desiccation, while the secretory trichomes maintain their secretory activity at different stages of leaf development, protecting different regions of the leaf against pathogens and herbivores.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10204781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The quality evaluation of the laurel leaf samples sold in the market under the name of "Defne Yaprağı". 以“Defne Yaprağı”的名义在市场上销售的月桂叶样品的质量评估。

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-10-10 DOI: 10.1007/s00709-023-01897-9

Gülsen Kendir, Gülmira Özek, Ayşegül Köroğlu, Temel Özek

{"title":"The quality evaluation of the laurel leaf samples sold in the market under the name of \"Defne Yaprağı\".","authors":"Gülsen Kendir, Gülmira Özek, Ayşegül Köroğlu, Temel Özek","doi":"10.1007/s00709-023-01897-9","DOIUrl":"10.1007/s00709-023-01897-9","url":null,"abstract":"Laurus nobilis L. is an aromatic shrub or tree, Mediterranean element. The leaves are employed as a spice and for medicinal purposes. It is known by various names in Turkey, such as \"defne, har and tehnel.\" In this study, 15 different laurel leaf samples were purchased from 6 different cities in Turkey, diagnosed, and evaluated in terms of quality. The conditions of use and sale of these samples were evaluated. The essential oils obtained from the leaf samples were analyzed, and their physical properties such as density, refractive index, and optical rotation were determined. In the chemical composition of the oils, 1,8-cineole (41.2-64.4%), sabinene (1.5-15.9%), and α-terpinyl acetate (1.5-15%) have been found to be the major components. However, in one station, the presence of 1,8-cineole was not be determined. Laurel leaf drugs offered for sale in some provinces of Turkey were purchased from the market and their morphological characteristics and essential oil profiles were examined and evaluated in terms of public health. It was determined that these samples should be standardized and quality-controlled before being released to the market.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41210699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The thioredoxin h-type TdTrxh2 protein of durum wheat confers abiotic stress tolerance of the transformant Arabidopsis plants through its protective role and the regulation of redox homoeostasis. 硬粒小麦的硫氧还蛋白h型TdTrxh2蛋白通过其保护作用和氧化还原稳态的调节赋予转化拟南芥植物非生物胁迫耐受性。

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-10-14 DOI: 10.1007/s00709-023-01899-7

Hanen Kamoun, Kaouthar Feki, Sana Tounsi, Olfa Jrad, Faiçal Brini

{"title":"The thioredoxin h-type TdTrxh2 protein of durum wheat confers abiotic stress tolerance of the transformant Arabidopsis plants through its protective role and the regulation of redox homoeostasis.","authors":"Hanen Kamoun, Kaouthar Feki, Sana Tounsi, Olfa Jrad, Faiçal Brini","doi":"10.1007/s00709-023-01899-7","DOIUrl":"10.1007/s00709-023-01899-7","url":null,"abstract":"The thioredoxins (Trxs) are ubiquitous and they play a crucial role in various biological processes like growth and stress response. Although the functions of Trxs proteins are described in several previous reports, the function of the isoform Trxh2 of durum wheat (Triticum durum L.), designated as TdTrxh2, in abiotic stress response still unknown. Thus, we aimed in this study the functional characterization of TdTrxh2 through its expression in yeast cells and Arabidopsis plants. Sequence analysis revealed that TdTrxh2 protein shared the conserved redox site with the other Trxh from other plant species. Under various abiotic stresses, TdTrxh2 was up-regulated in leaves and roots of durum wheat. Interestingly, we demonstrated that TdTrxh2 exhibit protective effect on LDH activity against various treatments. Besides, the expression of TdTrxh2 in yeast cells conferred their tolerance to multiple stresses. Moreover, transgenic Arabidopsis expressing TdTrxh2 showed tolerance phenotype to several abiotic stresses. This tolerance was illustrated by high rate of proline accumulation, root proliferation, low accumulation of reactive oxygen species like H2O2 and O2·-, and high antioxidant CAT and POD enzymes activities. All these findings suggested that TdTrxh2 promotes abiotic stress tolerance through the redox homoeostasis regulation and its protective role.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41210700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mating- and oviposition-dependent changes of the spermatheca and colleterial glands in the pest termite Cryptotermes brevis (Blattaria, Isoptera, Kalotermitidae). 有害白蚁布氏隐翅白蚁（Blattaria，等翅目，白蚁科）的精巢和鞘腺随交配和产卵而发生的变化。

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-09-02 DOI: 10.1007/s00709-023-01891-1

Iago Bueno da Silva, Ana Maria Costa-Leonardo

{"title":"Mating- and oviposition-dependent changes of the spermatheca and colleterial glands in the pest termite Cryptotermes brevis (Blattaria, Isoptera, Kalotermitidae).","authors":"Iago Bueno da Silva, Ana Maria Costa-Leonardo","doi":"10.1007/s00709-023-01891-1","DOIUrl":"10.1007/s00709-023-01891-1","url":null,"abstract":"The spermatheca and colleterial glands of female insects are organs associated with the reproductive system, responsible for sperm storage and secretion of egg coverings, respectively. Here we compared the development, secretory activity, and chemical nature of the secretion in the spermatheca and colleterial glands of different-aged females of the drywood termite Cryptotermes brevis. We also provide the ultrastructure of these organs in alate females. These structures have been poorly investigated in termites when compared to other eusocial insects (Hymenoptera) and termite-related dictyopterans (mantises and cockroaches). The spermatheca of C. brevis comprises a cone-shaped structure, connected to the genital chamber by a short duct. The colleterial glands, in turn, are divided into anterior and posterior tubules, each showing a basal trunk, and join into a common duct. Histological and histochemical analyses showed that the secretion of proteins and polysaccharides by the spermatheca takes place before pairing, but increases as females mate and store sperm. Colleterial glands of alates showed non-synchronous secretory activity, but the synthesis of products increased in egg-laying queens, together with the epithelium height. Ultrastructure of the spermatheca and colleterial glands revealed epithelia composed of class III secretory cells. Richness of mitochondria and electron-dense secretion in the spermatheca indicates synthesis and transport of content. Presence and absence of colleterial gland secretion in different individuals may reflect variable maturation stages of the females and secretory cells. Assuming that termites are iteroparous, the development and secretion of the spermatheca and colleterial glands play a crucial role for C. brevis queens.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10137828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water stress modulates terpene biosynthesis and morphophysiology at different ploidal levels in Lippia alba (Mill.) N. E. Brown (Verbenaceae). 水胁迫调节 Lippia alba (Mill.) N. E. Brown（马鞭草科）不同浆体水平的萜烯生物合成和形态生理学。

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-09-04 DOI: 10.1007/s00709-023-01890-2

Juliana Mainenti Leal Lopes, Laís Stehling de Queiroz Nascimento, Vinicius Carius Souza, Elyabe Monteiro de Matos, Evandro Alexandre Fortini, Richard Michael Grazul, Marcelo Oliveira Santos, Douglas E Soltis, Pamela S Soltis, Wagner Campos Otoni, Lyderson Facio Viccini

{"title":"Water stress modulates terpene biosynthesis and morphophysiology at different ploidal levels in Lippia alba (Mill.) N. E. Brown (Verbenaceae).","authors":"Juliana Mainenti Leal Lopes, Laís Stehling de Queiroz Nascimento, Vinicius Carius Souza, Elyabe Monteiro de Matos, Evandro Alexandre Fortini, Richard Michael Grazul, Marcelo Oliveira Santos, Douglas E Soltis, Pamela S Soltis, Wagner Campos Otoni, Lyderson Facio Viccini","doi":"10.1007/s00709-023-01890-2","DOIUrl":"10.1007/s00709-023-01890-2","url":null,"abstract":"Monoterpenes are the main component in essential oils of Lippia alba. In this species, the chemical composition of essential oils varies with genome size: citral (geraniol and neral) is dominant in diploids and tetraploids, and linalool in triploids. Because environmental stress impacts various metabolic pathways, we hypothesized that stress responses in L. alba could alter the relationship between genome size and essential oil composition. Water stress affects the flowering, production, and reproduction of plants. Here, we evaluated the effect of water stress on morphophysiology, essential oil production, and the expression of genes related to monoterpene synthesis in diploid, triploid, and tetraploid accessions of L. alba cultivated in vitro for 40 days. First, using transcriptome data, we performed de novo gene assembly and identified orthologous genes using phylogenetic and clustering-based approaches. The expression of candidate genes related to terpene biosynthesis was estimated by real-time quantitative PCR. Next, we assessed the expression of these genes under water stress conditions, whereby 1% PEG-4000 was added to MS medium. Water stress modulated L. alba morphophysiology at all ploidal levels. Gene expression and essential oil production were affected in triploid accessions. Polyploid accessions showed greater growth and metabolic tolerance under stress compared to diploids. These results confirm the complex regulation of metabolic pathways such as the production of essential oils in polyploid genomes. In addition, they highlight aspects of genotype and environment interactions, which may be important for the conservation of tropical biodiversity.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10137913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gametophytic self-incompatibility in Maxillariinae orchids. Maxillariinae兰花的配子体自交不亲和性。

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-10-03 DOI: 10.1007/s00709-023-01895-x

Nícolas Alberto Polizelli Ricci, João Pedro Silvério Pena Bento, Juliana Lischka Sampaio Mayer, Rodrigo Bustos Singer, Samantha Koehler

{"title":"Gametophytic self-incompatibility in Maxillariinae orchids.","authors":"Nícolas Alberto Polizelli Ricci, João Pedro Silvério Pena Bento, Juliana Lischka Sampaio Mayer, Rodrigo Bustos Singer, Samantha Koehler","doi":"10.1007/s00709-023-01895-x","DOIUrl":"10.1007/s00709-023-01895-x","url":null,"abstract":"Gametophytic self-incompatibility (GSI) has been mainly described in species-rich clades within Orchidaceae. We report GSI for a genus within Maxillariinae, one of the most conspicuous and diverse subtribes of neotropical orchids. Here, we describe the reproductive system of Brasiliorchis picta, B. phoenicanthera, and B. porphyrostele. Anatomical studies of fruit development showed that pollen tubes of aborted, self-pollinated flowers degenerate half-way in the stylar channel and never reach the ovules. Spontaneous self-pollination and emasculation set no fruits for none of the sampled species, thus supporting the hypothesis that these three species studied rely on the agency of pollinators and pollinator-mediated cross-pollination to set fruit. Fruit set from cross-pollinations ranged from 33.4 to 77.5%. One self-pollinated fruit of B. porphyrostele developed to completion. All other fruits aborted between 10 and 21 days after pollination. These data support previous evidence of variable strength GSI being exhibited in orchid species. Additional studies of self-incompatibility systems are needed to evaluate their role in species diversification and evolution of reproductive strategies in Maxillariinae and to allow for effective conservation strategies of threatened orchid species.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41156913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration in the callogenesis, tropane alkaloid formation, and gene expression in Hyoscyamus niger under clinorotation. 斜旋条件下黑山芋胼胝体发生、tropane生物碱形成和基因表达的变化。

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-10-09 DOI: 10.1007/s00709-023-01894-y

Sara Pourhabibian, Alireza Iranbakhsh, Mostafa Ebadi, Halimeh Hassanpour, Azadeh Hekmat

{"title":"Alteration in the callogenesis, tropane alkaloid formation, and gene expression in Hyoscyamus niger under clinorotation.","authors":"Sara Pourhabibian, Alireza Iranbakhsh, Mostafa Ebadi, Halimeh Hassanpour, Azadeh Hekmat","doi":"10.1007/s00709-023-01894-y","DOIUrl":"10.1007/s00709-023-01894-y","url":null,"abstract":"This study aimed to investigate the effects of clinorotation induced by 2-D clinostat on the growth, tropane alkaloid production, gene expression, antioxidant capacity, and cellular defense responses in the callus tissue of Hyoscyamus niger. Callus induction was conducted by putting hypocotyl explants in the MS culture medium supplemented with 1 mgL-1 2,4-D and 1 mgL-1 BAP growth regulators. The sub-cultured calli were placed on a clinostat for 0, 3, 7, and 10 days (2.24 × 10-5 g on the edge of the callus ring). Clinorotation significantly increased callus fresh weight, dry weight, protein, carbohydrate, and proline contents compared to the control, and their maximum contents were obtained after 7 and 10 days. H2O2 level enhanced under clinorotation with a 76.3% rise after 10 days compared to control and positively affected the atropine (77.1%) and scopolamine (69.2%) productions. Hyoscyamine 6-beta hydroxylase and putrescine N-methyltransferase gene expression involved in the tropane alkaloid biosynthesis were upregulated markedly with 14.2 and 17.1-folds increase after 10 days of clinorotation, respectively. The expressions of jasmonic acid, mitogen-activated protein kinase, and ethylene-responsive element-binding transcription factor were upregulated, and the activity of peroxidase and catalase showed a 72.7 and 80% rise after 10 days. These findings suggest that microgravity can enhance callogenesis by stimulating the ROS level, which can impact the antioxidant enzymes, tropane alkaloid formation, and gene expression.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of an efficient callus transient transformation system for Vitis vinifera cv. 'Chardonnay'. 葡萄品种霞多丽愈伤组织瞬时转化体系的建立。

IF 2.9 3区生物学

Protoplasma Pub Date : 2024-03-01 Epub Date: 2023-10-31 DOI: 10.1007/s00709-023-01901-2

Jieping Wu, Junxia Zhang, Xinyi Hao, Kai Lv, Yaping Xie, Weirong Xu

{"title":"Establishment of an efficient callus transient transformation system for Vitis vinifera cv. 'Chardonnay'.","authors":"Jieping Wu, Junxia Zhang, Xinyi Hao, Kai Lv, Yaping Xie, Weirong Xu","doi":"10.1007/s00709-023-01901-2","DOIUrl":"10.1007/s00709-023-01901-2","url":null,"abstract":"Grape (Vitis L.), a highly valued fruit crop, poses significant challenges in genetic transformation and functional characterization of genes. Therefore, there is an urgent need for the development of a rapid and effective method for grape transformation and gene function identification. Here, we introduce a streamlined Agrobacterium-mediated transient transformation system for grape calli. Optimal conditions were established with a leaf-derived callus induction medium; chiefly B5 medium supplemented with 0.05 mg/L NAA, 0.5 mg/L 2,4-D, and 2.0 mg/L KT; and a callus proliferation medium (B5 medium supplemented with 0.5 mg/L NAA and 2.0 mg/L 6-BA), respectively. Notably, GUS enzyme activity peaked (352.96 ± 33.95 mol 4-MU/mg/min) by sonication with Agrobacterium tumefaciens EHA105 and 100 μM AS for 4 min, followed by vacuum infection for 5 min, and co-culture at 25 °C in the dark for 1 day using callus as explants at an optical density (OD600) of 0.8. VaCIPK18 gene was transiently transformed into calli, and transcripts of the gene (endogenous and exogenous) were detected at higher levels than in non-transformed calli (endogenous). Moreover, after 10 days of treatment at 4 °C or -4 °C, the callus net weight of transformed callus was significantly higher than that of the untransformed callus, indicating that the VaCIPK18-overexpressing grape callus could improve cold tolerance. Overall, we establish a simple but effective transient transformation approach for grape callus, which could serve as a useful tool for the rapid assessment of gene function in this important crop.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71426278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0