IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-09 DOI: 10.1007/s00709-026-02209-7

Оlena M Nedukha

引用次数: 0

Bioinformatics analysis and differential tissue expression of the IGFBP4 gene in beef cattle. 肉牛IGFBP4基因的生物信息学分析及组织差异表达。

IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-06 DOI: 10.1007/s00709-026-02207-9

Ruirui Song, Bingzhi Li, Sihu Wang, Maryam Alenize, Yanqing Zhao, Aaishah M H Kaabi, Afnan O Deebani, Wasimah AlShammari, Dalal Alenizi, Dalal Alardan, Wenzhen Zhang

{"title":"Bioinformatics analysis and differential tissue expression of the IGFBP4 gene in beef cattle.","authors":"Ruirui Song, Bingzhi Li, Sihu Wang, Maryam Alenize, Yanqing Zhao, Aaishah M H Kaabi, Afnan O Deebani, Wasimah AlShammari, Dalal Alenizi, Dalal Alardan, Wenzhen Zhang","doi":"10.1007/s00709-026-02207-9","DOIUrl":"https://doi.org/10.1007/s00709-026-02207-9","url":null,"abstract":"IGFBP4 is essential for adipogenesis and is highly expressed in adipocytes and osteoblasts of mice. However, its role in bovine adipogenesis, especially intramuscular adipogenesis, remains unknown. Here, we cloned the CDS of IGFBP4 from Qinchuan cattle and analyzed its protein structure and function. We measured its expression levels in various tissues. The CDS region of the bovine IGFBP4 gene was 777 bp long, encoding a protein of 259 amino acids. The in-silico analysis revealed sequence conservation of Bos mutus and Bos indicus, while variation was found in Mus musculus and Rattus norvegicus sequences. The physical and chemical properties of the IGFBP4 protein exhibited an isoelectric point of 7.10 and a molecular mass of 27.89 kDa. The protein was predicted to be hydrophilic and lacked a transmembrane structure, with potential phosphorylation and glycosylation sites. Interaction analysis suggested associations with several other proteins. Regarding tissue expression, the mRNA level of the IGFBP4 gene was highest in the liver, lowest in muscle, and significantly higher in subcutaneous fat compared to intramuscular fat in Qinchuan beef cattle. These findings indicate a potential role of theIGFBP4 gene in regulating bovine adipogenesis and lipid metabolism. This study provides a foundation for exploring the underlying molecular mechanism of intramuscular fat deposition in cattle.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoprotective strategies of Haematococcus lacustris in response to desiccation stress. 湖红球菌对干燥胁迫的光保护策略。

IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-04 DOI: 10.1007/s00709-026-02203-z

Thomas Roach, Ginga Shimakawa

{"title":"Photoprotective strategies of Haematococcus lacustris in response to desiccation stress.","authors":"Thomas Roach, Ginga Shimakawa","doi":"10.1007/s00709-026-02203-z","DOIUrl":"https://doi.org/10.1007/s00709-026-02203-z","url":null,"abstract":"Water loss is a major challenge for photosynthetic organisms. Most are prone to drought stress and only few can tolerate full desiccation. Here, we investigated regulation of photosynthetic electron flow during dehydration and rehydration in Haematococcus lacustris, a desiccation tolerant green alga. During dehydration, non-photochemical quenching (NPQ) increased for dissipating excess light energy, while light-use efficiency of photosystems II (PSII) and I (PSI) decreased. The reaction centre of PSI (P700) became electron-limited at its donor side, helping form photoprotective P700+. Inhibiting alternative oxidases with octyl gallate delayed chlorophyll fluorescence quenching, indicating that plastid terminal oxidases (PTOX) supported formation of NPQ during desiccation. Reduction rates of P700+ during a saturating pulse were slower if cells dehydrated slower, showing that photoprotection was upregulated during desiccation acclimation. During rehydration, octyl gallate and diphenyleneiodonium (DPI), a flavoenzyme inhibitor, slowed oxidation of P700 under actinic light, indicating PTOX and flavodiiron proteins (FLV) were involved in maintaining P700+. A similar response occurred with the protonophore nigericin. We conclude that beyond preventing over-reduction of the electron transport chain, PTOX and FLV facilitated thylakoid luminal acidification under low water stress, protecting photosystems via NPQ, photosynthetic control and P700+ formation.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147820048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eight weeks after anthesis: a critical fruit developmental stage for tissue-border establishment in oil palm tenera fruit. 花后8周：油棕果实组织边界建立的关键果实发育阶段。

IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-01 Epub Date: 2025-12-02 DOI: 10.1007/s00709-025-02139-w

Fong-Chin Lee, Wan-Chin Yeap, Po-Yee Fong, Ai-Ling Ong, Chee-Keng Teh

{"title":"Eight weeks after anthesis: a critical fruit developmental stage for tissue-border establishment in oil palm tenera fruit.","authors":"Fong-Chin Lee, Wan-Chin Yeap, Po-Yee Fong, Ai-Ling Ong, Chee-Keng Teh","doi":"10.1007/s00709-025-02139-w","DOIUrl":"10.1007/s00709-025-02139-w","url":null,"abstract":"Oil palm (Elaeis guineensis Jacq.) is the most productive oil crop, contributing to approximately 35% of the global vegetable oil production. The tenera fruit form, derived from dura × pisifera crosses, is planted for its superior mesocarp oil yield due to thicker mesocarp and thinner endocarp fruit characteristics. In this study, histology analyses were conducted to characterize the developmental progression in tenera fruit. Cytological and morphological analyses revealed that fruit tissue compartmentation commence as early as 3 weeks after anthesis (WAA), with the pericarp distinctly differentiating into exocarp, mesocarp, and endocarp layers that enclose the seed coat and endosperm. The endocarp layer becomes prominent at 4 WAA and reaches an average maximum thickness of 1.1 ± 0.55 mm until full ripening at 23 WAA. Sclereid secondary cell wall lignification is initiated at 8 WAA, coincides with the onset of endosperm cellularization and a significant increase in endocarp cell density. The expression of SHELL gene showed a developmental stage-dependent pattern with highest expression recorded at 12 WAA, aligning with the phase of active lignification detected histologically. Collectively, these findings identify 8 WAA as a critical developmental stage that defines seed size, endocarp lignification, shell thickness, endosperm cellularization, and mesocarp capacity for oil accumulation in tenera fruit. These coordinated cytological and molecular changes suggest a shared transcriptional regulation of tissue-border formation and cell wall biogenesis in oil palm fruit. This study provides a foundation for transcriptomic investigations into the molecular control of fruit tissue differentiation and size control, contributing to strategies to enhance oil yield, seed quality and fruit architecture in elite tenera palms.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"827-840"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13109130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145655442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic analysis of fecal microbial communities in dairy goats from different farms. 不同养殖场奶山羊粪便微生物群落的宏基因组分析。

IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-01 Epub Date: 2026-02-16 DOI: 10.1007/s00709-026-02160-7

Liang Chengcheng, Zhou Yanduo, Wang Zhebin, Li Jianzhang, Zhu Yangtao, Li Jun, Li Yu, Hashim R Felemban, Othman Yahya Alyahyawy, Ahmad F Alhomodi, Fatimah Hadadi, Ali Shaibah, Li Bingzhi, Wang Xianwei

引用次数: 0

Sem-based characterization of Psylliodes valida weise, 1889 (Galerucinae: Alticini): aedeagus and spermatheca in high detail. 基于扫描电镜的验证木，1889 (Galerucinae: Alticini): aedeagus和精囊的详细表征。

IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-01 Epub Date: 2025-12-11 DOI: 10.1007/s00709-025-02140-3

Neslihan Bal

引用次数: 0

Biogenic iron oxide nanoparticles synthesized using Trichoderma spp.: A sustainable approach for fusarium wilt management in tomato. 利用木霉合成生物源氧化铁纳米颗粒：番茄枯萎病的可持续治理方法。

IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-01 Epub Date: 2026-01-07 DOI: 10.1007/s00709-025-02150-1

Hetvi Naik, Salim Manoharadas, Narayanasamy Bommayasamy, John Thomas, Muthukaruppan Gobi, Muthu Thiruvengadam, Natarajan Amaresan

{"title":"Biogenic iron oxide nanoparticles synthesized using Trichoderma spp.: A sustainable approach for fusarium wilt management in tomato.","authors":"Hetvi Naik, Salim Manoharadas, Narayanasamy Bommayasamy, John Thomas, Muthukaruppan Gobi, Muthu Thiruvengadam, Natarajan Amaresan","doi":"10.1007/s00709-025-02150-1","DOIUrl":"10.1007/s00709-025-02150-1","url":null,"abstract":"Fusarium wilt caused by Fusarium oxysporum poses a significant threat to tomato cultivation worldwide and requiring the development of sustainable and effective control measures. This study explored the potential of biologically synthesized iron oxide nanoparticles (IONPs) as an eco-friendly alternative to conventional fungicides and evaluated their antifungal efficacy along with plant health impact. The IONPs were synthesized using Trichoderma spp. metabolites and characterized using multiple analytical techniques. DLS and XRD analyses confirmed a uniform size distribution (80 nm hydrodynamic size and 16.83 nm crystallite size). SEM imaging showed agglomerated particles ranging from 30 to 50 nm, while EDX confirmed Fe and O as the primary elements. FTIR spectroscopy indicated the presence of protein capping agents and Fe-O bonds. In vitro studies demonstrated significant antifungal activity with 76.19% growth inhibition against F. oxysporum compared to commercial carbendazim (17.86%). Greenhouse experiments showed dose-dependent effects with 100 ppm IONPs treatment resulted in remarkable improvements in plant growth parameters: 143.1% increase in shoot length, 178.8% in root length, and 505.9% in wet weight. Disease incidence was reduced by 89.8% at a concentration of 100 ppm, whereas the disease severity index decreased by 60%. Plant physiological analysis revealed an enhanced chlorophyll content (237.3% increase at 100 ppm) and modulated stress enzyme (superoxide dismutase and peroxidase) activity. The soil dehydrogenase activity improved by 170% after treatment with 100 ppm. Furthermore, toxicity prediction indicated a low toxicity potential (LD50: 5000 mg/kg, class 4), supporting it's safe agricultural applications. This study highlights biosynthesized IONPs as an eco-friendly alternative for Fusarium wilt management, offering potential in sustainable agriculture.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"797-810"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulose-based nanomaterials: innovations in wound healing and regenerative medicine. 纤维素基纳米材料：伤口愈合和再生医学的创新。

IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-01 Epub Date: 2025-12-19 DOI: 10.1007/s00709-025-02143-0

Kiran Dudhat, Ishaan Garach, Dhaval Mori

引用次数: 0

Artificial MicroRNA delivery and gene silencing via extracellular vesicles derived from molecularly modified tobacco. 通过分子修饰烟草细胞外囊泡的人工MicroRNA传递和基因沉默。

IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-01 Epub Date: 2025-12-10 DOI: 10.1007/s00709-025-02130-5

Yury Shkryl, Zhargalma Tsydeneshieva, Tatiana Gorpenchenko, Vitaly Kazarin, Olesya Kudinova, Victor Bulgakov, Yulia Yugay

引用次数: 0

Comparative analysis of the biochemical composition of fruit tissues in representatives of the Malus sect. Chloromeles. 标题苹果属代表性植物果实组织生化成分的比较分析。

IF 2.5 3区生物学

Protoplasma Pub Date : 2026-05-01 Epub Date: 2025-12-10 DOI: 10.1007/s00709-025-02129-y

Elena Rudikovskaya, Lyubov Dudareva, Zlata Stavitskaya, Lyudmila Vanina, Igor Gorbenko, Alexander Rudikovskii

{"title":"Comparative analysis of the biochemical composition of fruit tissues in representatives of the Malus sect. Chloromeles.","authors":"Elena Rudikovskaya, Lyubov Dudareva, Zlata Stavitskaya, Lyudmila Vanina, Igor Gorbenko, Alexander Rudikovskii","doi":"10.1007/s00709-025-02129-y","DOIUrl":"10.1007/s00709-025-02129-y","url":null,"abstract":"This research represents the first comprehensive biochemical analysis of mature fruits from apple trees of the Chloromeles section, specifically examining Malus coronaria, Malus ioensis, Malus × platycarpa and Malus × soulardii. The experimental investigations revealed distinctive compositional characteristics in lipids, carbohydrates, flavonoids, and chlorophylls that markedly differentiate these species from other representatives of the genus Malus. Specifically, our research revealed the absence of procyanidin B1 and a high content of chlorogenic acid, accompanied by remarkably low quantities of cinnamic acid and its derivatives. Linoleic acid represented approximately 50% of the total fatty acid profile, which is substantially lower compared to the lipid content commonly observed in domesticated apple varieties. Conversely, the linolenic acid concentration was significantly higher. Fructose levels in fruit tissues of the Chloromeles section ranged from 15% in M. ioensis to 50% in M. coronaria, the section's largest fruiting species. Additionally, the analysis revealed the presence of transport sugars and highly etherified protopectins. Unlike other Malus, the Chloromeles section mature fruits exhibited high chlorophyll and low carotenoid contents. These pigments were present in both the peel and pulp tissues. The observed composition, including elevated transport sugar and protopectin levels, is typically associated with fruits at early ripening stages. This may contribute to the suboptimal flavor development, despite the presence of a relatively high total soluble sugar content.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"861-873"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145715500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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