IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-07 DOI: 10.1007/s00709-024-02003-3

Niaboula Dembele, Aimé Ainin Somboro, Nah Traore, Mamadou Badiaga, Salimatou Cisse, Mody Cisse, Peter Nick

{"title":"Anti-microtubular activity of total alkaloids and aqueous extract of Detarium microcarpum a medicinal plant harvested in Mali.","authors":"Niaboula Dembele, Aimé Ainin Somboro, Nah Traore, Mamadou Badiaga, Salimatou Cisse, Mody Cisse, Peter Nick","doi":"10.1007/s00709-024-02003-3","DOIUrl":"https://doi.org/10.1007/s00709-024-02003-3","url":null,"abstract":"Detarium microcarpum, is a species confined to drier regions of west and central Africa used to treat various diseases including cancer. Phytochemical screening revealed the presence of secondary metabolites (alkaloids) The aim of this work is to study the effect of total aqueous extracts and alkaloid fractions from D. microcarpum leaves, bark and roots on Nicotiana tabacum L. cv. 'Bright Yellow 2' (BY-2) tobacco cell line GFP-TuA3 expressing a N-terminal fusion of GFP. The plant was harvested in two different regions of Mali with a contrasting climate. The effects of the extracts on the microtubules was followed by spinning disc confocal microscopy. We showed that the anti-microtubular effect of the extracts is dose-dependent, depends of the sampling site and the part of the plant used. Total alkaloids extracted of D. microcarpum bark have more effect on microtubules than leaf and root. The bioactivity-guided fractionation should be used to screen out the biologically active compounds of the total alkaloid extracts of the bark of D. microcarpum.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant intelligence dux: a comprehensive rebuttal of Kingsland and Taiz. 植物智能寓言：对金斯兰德和泰兹的全面反驳。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-06 DOI: 10.1007/s00709-024-02005-1

Anthony Trewavas

{"title":"Plant intelligence dux: a comprehensive rebuttal of Kingsland and Taiz.","authors":"Anthony Trewavas","doi":"10.1007/s00709-024-02005-1","DOIUrl":"https://doi.org/10.1007/s00709-024-02005-1","url":null,"abstract":"Intelligence is a fundamental property for all life enabling an increased probability of survival and reproduction under wild circumstances. Kingsland and Taiz (2024) think that plants are not intelligent but seem unaware of the extensive literature about intelligence, memory, learning and chromatin topology in plants. Their views are consequently rejected. Their claim of fake quotations is shown to result from faulty reasoning and lack of understanding of practical biology. Their use of social media as scholarly evidence is unacceptable. Darwin's views on intelligence are described, and their pertinence to the adaptive responses of plants is discussed. Justifications for comments I have made concerning McClintock and her \"thoughtful\" cell, von Sachs writings as indicating purpose (teleonomy) to plant behaviour, Went and Thimann's allusions to plant intelligence and Bose legacy as the father of plant electrophysiology are described. These scientists were usually first in their field of knowledge, and their understanding was consequently deeper. The article finishes with a brief critical analysis of the 36 scientists who were used to condemn plant neurobiology as of no use. It is concluded that participants signed up to a false prospectus because contrary evidence was omitted.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of the durum wheat TdHKT1;4-1 partially complements the mutant athkt1 in Arabidopsis thaliana under severe salt stress. 在拟南芥中异源表达硬粒小麦 TdHKT1;4-1 可部分补充严重盐胁迫下的突变体 athkt1。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-05 DOI: 10.1007/s00709-024-02006-0

Siwar Ben Amar, Faiçal Brini, Khaled Masmoudi

{"title":"Heterologous expression of the durum wheat TdHKT1;4-1 partially complements the mutant athkt1 in Arabidopsis thaliana under severe salt stress.","authors":"Siwar Ben Amar, Faiçal Brini, Khaled Masmoudi","doi":"10.1007/s00709-024-02006-0","DOIUrl":"https://doi.org/10.1007/s00709-024-02006-0","url":null,"abstract":"High-affinity K+ (HKT) transporters which mediate Na+-specific transport or Na+-K+ co-transport play a key role in plant salt tolerance. In our previous functional study in Xenopus oocytes, we demonstrated that the durum wheat TdHKT1;4-1 acts as a Na+-selective transporter. Here, we investigated the function of TdHKT1;4-1 and its contribution in salt stress tolerance in the Arabidopsis athkt1 mutant background. Our results revealed that TdHKT1;4-1 partially complements the salt sensitivity phenotype of the athkt1 transgenic lines. Comparative physiological analyses and oxidative stress status under moderate salt stress (50 mM NaCl) showed that both transgenic lines SH3 and SH5 restored the salt stress tolerance comparable to the level observed in Wt plants. Whereas, under severe salt stress treatment (100 mM NaCl), the athkt1 transgenic lines exhibited an intermediate salt stress tolerance between Wt and athkt1 mutant. Moreover, TdHKT1;4-1 was highly expressed in leaves under moderate and severe salt stress, while in roots, it was largely expressed only under severe salt stress. In addition, antioxidant enzymes such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were significantly expressed in SH3 and SH5 lines compared to athkt1 and Wt under moderate stress. Therefore, TdHKT1;4-1 seems to differ from its Arabidopsis homologous counterpart, as it contributes to salt stress tolerance up to a specific threshold, above which the TdHKT1;4-1 expression may lead to higher root Na+ influx, hence increasing its toxicity during salt stress.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytogenetic analysis of meiotic behaviour and stability in a trigeneric hybrid (triticale x trigopiro). 三系杂交种（三尖杉 x 三尖杉）减数分裂行为和稳定性的细胞遗传学分析。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-07-02 DOI: 10.1007/s00709-024-01964-9

M Fradkin, E J Greizerstein, E Grassi, V Ferreira, M R Ferrari, L Poggio

{"title":"Cytogenetic analysis of meiotic behaviour and stability in a trigeneric hybrid (triticale x trigopiro).","authors":"M Fradkin, E J Greizerstein, E Grassi, V Ferreira, M R Ferrari, L Poggio","doi":"10.1007/s00709-024-01964-9","DOIUrl":"10.1007/s00709-024-01964-9","url":null,"abstract":"Trigeneric hybrids in Triticeae may help to establish evolutionary relationships among different genomes present in the same cellular genetic background and to transfer different alien characters into cultivated wheat. In the present study, a trigeneric hybrid involving species of Triticum, Secale, and Thinopyrum was synthesized by crossing hexaploid triticale with hexaploid trigopiro. The meiotic behaviour of chromosomes belonging to different genomes was analyzed, using routine and in situ hybridization techniques in F1, F2, and F3 generations of the trigeneric hybrid. The purpose of this study was to determine the chromosome number and genomic constitution and to discuss the mechanisms involved in the stabilization of the artificial tricepiro hybrids. The chromosome number of the trigeneric F1 hybrid was 2n = 42. Between 12 and 16 bivalents were observed in the central zone of the equatorial meiotic plate and between 9 and 18 univalents were found in the periphery of the MI equatorial plate. Seven of these univalents showed hybridization signals with rye DNA. Lagging rye and non-rye chromosomes and separation of sister chromatids were found in anaphase I. Tetrads with a maximum of six micronuclei, with and without hybridization signals of rye DNA, were observed. After three generations, meiotic cells revealed the presence of 42 chromosomes and 21 bivalents in diakinesis cells. The presence of 14 rye (Secale cereale) chromosomes and the complete pairing of chromosomes in F3 hybrids suggest that rye chromosomes would be preferentially transmitted to the progeny and that an elimination mechanism would act on chromosomes of Thinopyrum and wheat D genome.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141493177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of pollen wall development in Lysimachia vulgaris. Lysimachia vulgaris 的花粉壁发育机制。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-07-22 DOI: 10.1007/s00709-024-01970-x

Nina I Gabarayeva, Valentina V Grigorjeva, Dmitri A Britski

{"title":"Mechanisms of pollen wall development in Lysimachia vulgaris.","authors":"Nina I Gabarayeva, Valentina V Grigorjeva, Dmitri A Britski","doi":"10.1007/s00709-024-01970-x","DOIUrl":"10.1007/s00709-024-01970-x","url":null,"abstract":"Exine, this complex sporopollenin-containing and highly variable among taxa envelope of the male gametophyte, consists of two layers, ectexine and endexine. We traced in detail the pollen wall development in Lysimachia vulgaris (Primulaceae), with emphasis on driving forces and critical ontogenetic time. By observation on the sequence of the emergent patterns and by analysis of their substructure with TEM, we intended to clarify the obvious and not-obvious ways of exine construction and to find out the common features in pattern development in other representatives in living nature. The ectexine and endexine ontogeny follows the main stages observed in many other species: first, the appearance of microspore plasma membrane invaginations with isotropic contents within, changed later to anisotropic state; then successive appearance of spherical, rod-like, and lamellate units in the periplasmic space. The lamellate endexine appears unusually early in the exine development. All these elements and their aggregations are manifestation of well-known physical phenomena: phase separation and micellar self-assembly. A consideration of similar surface patterns in very remote taxa suggests the participation in their development of some general nature phenomena as the lows of space-filling operations.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organic extracts from sustainable hybrid poplar hairy root cultures as potential natural antimicrobial and antibiofilm agents. 从可持续杂交白杨毛根培养物中提取有机提取物，作为潜在的天然抗菌剂和抗生物膜剂。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-07-26 DOI: 10.1007/s00709-024-01971-w

Sonia Malik, Gowtham Kumaraguru, Margot Bruat, Françoise Chefdor, Christiane Depierreux, François Héricourt, Sabine Carpin, Girija Shanmugam, Frédéric Lamblin

{"title":"Organic extracts from sustainable hybrid poplar hairy root cultures as potential natural antimicrobial and antibiofilm agents.","authors":"Sonia Malik, Gowtham Kumaraguru, Margot Bruat, Françoise Chefdor, Christiane Depierreux, François Héricourt, Sabine Carpin, Girija Shanmugam, Frédéric Lamblin","doi":"10.1007/s00709-024-01971-w","DOIUrl":"10.1007/s00709-024-01971-w","url":null,"abstract":"In order to meet growing consumer demands in terms of naturalness, the pharmaceutical, food, and cosmetic industries are looking for active molecules of plant origin. In this context, hairy roots are considered a promising biotechnological system for the sustainable production of compounds of interest. Poplars (genus Populus, family Salicaceae) are trees of ecological interest in temperate alluvial forests and are also cultivated for their industrial timber. Poplar trees also produce specialized metabolites with a wide range of bioactive properties. The present study aimed to assess the hybrid poplar hairy root extracts for antimicrobial and antibiofilm activities against four main life-threatening strains of Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria. Ethyl acetate extracts from two hairy root lines (HP15-3 and HP A4-12) showed significant antibacterial properties as confirmed by disc diffusion assay. Antibiofilm activities were found to be dose dependent with significant biofilm inhibition (75-95%) recorded at 1000 µg.mL-1 in all the bacterial strains tested. Dose-dependent enhancement in the release of exopolysaccharides was observed in response to treatment with extracts, possibly because of stress and bacterial cell death. Fluorescence microscopy confirmed loss of cell viability of treated bacterial cells concomitant with increased production of reactive oxygen species compared to the untreated control. Overall, this study demonstrates for the first time a high potential of poplar hairy root extracts as a natural and safe platform to produce antimicrobial agents in pharmaceutical, food, industrial water management, or cosmetic industries.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141767132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of the processes involved in the loss of elaiophores in two species of the christianelloid clade (Malpighiaceae). 比较分析蝶形花科（Malpighiaceae）两个物种失去elaiophores的过程。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-06-01 DOI: 10.1007/s00709-024-01960-z

Sofía D Reposi, Magalí R Nicolau, Marina M Gotelli, Sandra S Aliscioni, Juan P Torretta

{"title":"Comparative analysis of the processes involved in the loss of elaiophores in two species of the christianelloid clade (Malpighiaceae).","authors":"Sofía D Reposi, Magalí R Nicolau, Marina M Gotelli, Sandra S Aliscioni, Juan P Torretta","doi":"10.1007/s00709-024-01960-z","DOIUrl":"10.1007/s00709-024-01960-z","url":null,"abstract":"Alicia anisopetala and Callaeum psilophyllum are two closely related species that belong to the christianelloid clade of the family Malpighiaceae. Both species are pollinated by oil-collecting bees and exhibit variations at specimen and population level in the number of elaiophores per flower. These floral glands that secrete non-volatile oils constitute an ancestral trait for the family. There is evidence that the observed variations in the number of elaiophores can be the result of processes of connation or reduction associated with differences in their vascularization. In order to identify which process occurs in each species, we conducted an anatomical study in natural populations of both species distributed along a wide range of their geographical distributions in Argentina. We collected flowers of different individuals, counted the number of elaiophores per flower, carried out exomorphological observations, and used conventional histological techniques to examine the vascularization of these glands. The floral anatomy of both species does not show any modifications in other whorls related to the fusion or reduction of elaiophores. Our results indicate that the process of loss of elaiophores in A. anisopetala is caused by incomplete connation and in C. psilophyllum by reduction, suggesting that the processes that lead to the loss of elaiophores in Malpighiaceae are homoplastic and would not reflect phylogenetic signals.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141184396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemically induced phenotype plasticity in the unicellular zygnematophyte, Penium margaritaceum. 化学诱导的单细胞齐根叶藻（Penium margaritaceum）的表型可塑性。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-07-05 DOI: 10.1007/s00709-024-01962-x

Josephine G LoRicco, Kaylee Bagdan, Gabriel Sgambettera, Stuart Malone, Tawn Tomasi, Iris Lu, David S Domozych

{"title":"Chemically induced phenotype plasticity in the unicellular zygnematophyte, Penium margaritaceum.","authors":"Josephine G LoRicco, Kaylee Bagdan, Gabriel Sgambettera, Stuart Malone, Tawn Tomasi, Iris Lu, David S Domozych","doi":"10.1007/s00709-024-01962-x","DOIUrl":"10.1007/s00709-024-01962-x","url":null,"abstract":"Phenotypic plasticity allows a plant cell to alter its structure and function in response to external pressure. This adaptive phenomenon has also been important in the evolution of plants including the emergence of land plants from a streptophyte alga. Penium margaritaceum is a unicellular zygnematophyte (i.e., the group of streptophyte algae that is sister to land plants) that was employed in order to study phenotypic plasticity with a focus on the role of subcellular expansion centers and the cell wall in this process. Live cell fluorescence labeling, immunofluorescence labeling, transmission electron microscopy, and scanning electron microscopy showed significant subcellular changes and alterations to the cell wall. When treated with the actin-perturbing agent, cytochalasin E, cytokinesis is arrested and cells are transformed into pseudo-filaments made of up to eight or more cellular units. When treated with the cyclin-dependent kinase (CDK) inhibitor, roscovitine, cells converted to a unique phenotype with a narrow isthmus zone.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141535133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vital stress in animals and plants. 动物和植物的生命压力

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-10-17 DOI: 10.1007/s00709-024-02001-5

Peter Nick

引用次数: 0

Cultivation modes affect the morphology, biochemical composition, and antioxidant and anti-inflammatory properties of the green microalga Neochloris oleoabundans. 栽培模式影响绿色微藻 Neochloris oleoabundans 的形态、生化成分以及抗氧化和抗炎特性。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-06-12 DOI: 10.1007/s00709-024-01958-7

C Baldisserotto, S Gessi, E Ferraretto, S Merighi, L Ardondi, P Giacò, L Ferroni, M Nigro, A Travagli, S Pancaldi

{"title":"Cultivation modes affect the morphology, biochemical composition, and antioxidant and anti-inflammatory properties of the green microalga Neochloris oleoabundans.","authors":"C Baldisserotto, S Gessi, E Ferraretto, S Merighi, L Ardondi, P Giacò, L Ferroni, M Nigro, A Travagli, S Pancaldi","doi":"10.1007/s00709-024-01958-7","DOIUrl":"10.1007/s00709-024-01958-7","url":null,"abstract":"Microalgae are considered promising sustainable sources of natural bioactive compounds to be used in biotechnological sectors. In recent years, attention is increasingly given to the search of microalgae-derived compounds with antioxidant and anti-inflammatory properties for nutraceutical or pharmacological issues. In this context, attention is usually focused on the composition and bioactivity of algae or their extracts, while less interest is driven to their biological features, for example, those related to morphology and cultivation conditions. In addition, specific studies on the antioxidant and anti-inflammatory properties of microalgae mainly concern Chlorella or Spirulina. The present work was focused on the characterization of the Chlorophyta Neochloris oleoabundans under two combinations of cultivation modes: autotrophy and glucose-induced mixotrophy, each followed by starvation. Biomass for morphological and biochemical characterization, as well as for extract preparation, was harvested at the end of each cultivation phase. Analyses indicated a different content of the most important classes of bioactive compounds with antioxidant/anti-inflammatory properties (lipids, exo-polysaccharides, pigments, total phenolics, and proteins). In particular, the most promising condition able to prompt the production of antioxidant algal biomass with anti-inflammatory properties was the mixotrophic one. Under mixotrophy, beside an elevated algal biomass production, a strong photosynthetic metabolism with high appression of thylakoid membranes and characteristics of high photo-protection from oxidative damage was observed and linked to the overproduction of exo-polysaccharides and lipids rather than pigments. Overall, mixotrophy appears a good choice to produce natural bioactive extracts, potentially well tolerated by human metabolism and environmentally sustainable.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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