Protoplasma最新文献

{"title":"A validation study by integrated analysis of physiological, biochemical, and meta-gene expression responses to drought stress in sorghum (Sorghum bicolor L.).","authors":"Hossein Kazemi, Atefeh Sabouri, Ali Aalami, Amin Abedi, Mahnaz Nezamivand-Chegini","doi":"10.1007/s00709-025-02112-7","DOIUrl":"https://doi.org/10.1007/s00709-025-02112-7","url":null,"abstract":"<p><p>Sorghum (Sorghum bicolor L.) is a prominent cereal known for its high photosynthetic efficiency and biomass production, serving as a source of food, animal feed, fiber, and biofuels. This study aims to validate identified meta-genes associated with drought stress in sorghum. Two cultivars, Mansour (drought-tolerant) and Pegah (drought-susceptible), were subjected to drought stress at four levels (25%, 50%, 75%, and 100% of field capacity [FC]( During the 4-5 leaf stage in a greenhouse in 2021. The physiological and molecular responses of the sorghum samples were evaluated at 24, 48, 72, and 96 h post-treatment. The expression of five meta-genes was analyzed to validate these candidate genes related to drought stress tolerance in sorghum. Analysis of variance indicated that the main effects of drought, cultivar, and sampling time, as well as their interactions, had highly significant effects (P < 0.01) on most physiological and biochemical traits. The relative expression of the genes SORBI_3002G225100, SORBI_3003G332200, SORBI_3003G368300, SORBI_3010G081800, and SORBI_3004G293500 increased over time under drought stress. Proline levels, ion leakage, soluble sugars, and the activities of catalase, peroxidase, ascorbate peroxidase, and superoxide dismutase enzymes increased with the intensity of drought stress and over time. Conversely, the levels of chlorophyll a and b, carotenoids, RWC, leaf surface area, and protein content decreased under drought conditions. These results confirm the relevance of these genes in conferring drought stress tolerance in sorghum. This research provides new finding into the physiological processes and biochemical activities, alongside the validation of meta-gene expression involved in drought stress, further advancing our understanding of molecular mechanisms of the reaction of sorghum to drought stress.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategies for protecting aboveground buds: role of secretory structures and morphoanatomical adaptations.","authors":"Rodrigo Faleiro, Juliana Lischka Sampaio Mayer","doi":"10.1007/s00709-025-02110-9","DOIUrl":"https://doi.org/10.1007/s00709-025-02110-9","url":null,"abstract":"<p><p>The Brazilian Cerrado stands as the most biodiverse neotropical savanna, supporting a vast array of endemic species uniquely adapted to its challenging environment. These species have evolved under strong selective pressures imposed by recurrent natural disturbances, including pronounced seasonal drought, intense herbivory, and frequent fire events. Consequently, Cerrado species have evolved diverse morpho-anatomical adaptations to persist in this fire-prone ecosystem. Resprouting capacity -whether from belowground or aboveground buds-represents a key functional trait for post-disturbance regeneration. While thick bark has long been recognized as the primary mechanism protecting aerial meristematic tissues, emerging research reveals finer-scale adaptations that complement bark defenses. This study investigates the protective features of aerial buds in two herbaceous Chamaecrista species (Fabaceae) to the Brazilian Cerrado. We employed a multi-technique anatomical approach combining light microscopy, histochemical analysis, fluorescence microscopy, and scanning electron microscopy (SEM). Our results revealed the presence of secretory structures, such as colleters and extrafloral nectaries, which develop at distinct temporal stages, indicating different phases of bud protection throughout plant development. Additionally, structures such as hairy leaf primordia with phenolic compound accumulation, stipules, and accessory buds were observed, reinforcing both structural and chemical investments in the protection of aerial buds. These structures confer high resilience to intense UV radiation and herbivory, providing the species with greater resistance and the ability to withstand various environmental disturbances. These findings demonstrate that herbaceous Cerrado species employ sophisticated, multi-layered strategies for aerial bud protection, comparable in complexity to woody species. The discovery of such refined adaptive mechanisms in herbaceous and subshrub growth forms challenges the traditional woody-centered paradigm of Cerrado resilience research.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosomal puzzle in snakes: adjacent interstitial telomeric sites on chromosome 5 in three species of genus Vipera.","authors":"Ilya Redekop, Victor Spangenberg","doi":"10.1007/s00709-025-02109-2","DOIUrl":"https://doi.org/10.1007/s00709-025-02109-2","url":null,"abstract":"<p><p>Large interstitial telomeric regions are considered remnants and markers of chromosomal rearrangements or a result of several suggested molecular mechanisms of telomere repeats accumulation. More rare are cases when large interstitial repeats are found not close to, but at a distance from the centromere. However, synapsis, recombination, and effects on chromatin near these regions during meiotic prophase I have not been sufficiently studied. Using the model of three snake species of the genus Vipera: V. berus, V. nikolskii, V. renardi, we studied interstitial telomere sites (ITSs) in the pachytene nuclei of primary spermatocytes. We discovered an unusual composite chromosome in the species under study with two ITSs located far from the centromere. In V. berus, two very large adjacent ITS blocks were found on bivalent 5. In the other two species, V. nikolskii and V. renardi, two ITSs are also present on bivalent 5, but they are significantly smaller and barely distinguishable by FISH on pachytene bivalents. The possibility of forming crossing-over sites is shown between the two ITSs. Apparently, the three studied viper species received this complex structure of chromosome 5 from their common ancestor. However, the transformation of these telomeric repeat regions during evolution in the species under study occurred differently. Possible mechanisms of modifications of the telomeric regions are discussed.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology of the larval midgut of the longhorn beetle Rhytidodera bowringii White, 1853 (Coleoptera: Cerambycidae: Cerambycinae).","authors":"Diao Zhu, Xiao-Yun Wang, Lu Liu, Xin Tong","doi":"10.1007/s00709-025-02108-3","DOIUrl":"10.1007/s00709-025-02108-3","url":null,"abstract":"<p><p>The midgut of insects originates from the endoderm. It is located in the central part of the digestive tract and serves as the primary site for chemical digestion and nutrient absorption. The larvae of Cerambycidae are the most destructive life stage. However, the ultrastructure of the larval midgut has been reported in only a few cerambycid groups. This study employs light, scanning electron, and transmission electron microscopy to observe the midgut ultrastructure of the Rhytidodera bowringii White, 1853 larvae. From outside to inside, the midgut of R. bowringii consists of a muscle layer, a basal membrane, an epithelium, and a lumen. The external muscles of the midgut are arranged in an outer longitudinal muscle and inner circular muscle. The epithelial tissue mainly comprises two types of cells: digestive cells and regenerative cells. The arrangement of regenerative cells in the midgut forms nidi, where multiple cells cluster together. The nucleus occupies most of the cytoplasm, which contains only a small number of organelles. The basal plasma membrane of the midgut epithelial cells is conspicuously infolded in R. bowringii, and around the nucleus, with a large amount of rough endoplasmic reticulum. At the apical regions of these digestive cells, numerous tightly arranged microvilli and mitochondria can be observed and many vesicles are localized near the lumen. This indicates that the digestive cells in the midgut have a strong secretory activity of digestive enzymes and other proteins, which may facilitate the larvae of the cerambycid in digesting recalcitrant plant tissues. It is the first time that the ultrastructure of the midgut of the R. bowringii larvae has been studied. The results can provide foundational insights into the ultrastructural organization of the Cerambycidae larval digestive system and the toxic mechanisms underlying prevention strategies for this pest.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural study of microspore and male gametophyte development in Senecio cannabifolius Less.","authors":"Fangdi Li, Xinyu Zhang, Shuo Zhang, Lulu Ma, Zhonghui Yue, Wei Li","doi":"10.1007/s00709-025-02051-3","DOIUrl":"10.1007/s00709-025-02051-3","url":null,"abstract":"<p><p>The genus Senecio, which is a basal angiosperm group, holds significant importance for evolutionary and phylogenetic research. It is notable for possessing male meiotic characteristics that are rarely observed in most angiosperms. However, the current understanding about male meiosis in Senecio remains incomplete. Here, we traced the cell morphological characteristics of microsporogenesis, and male gametophyte development of Senecio cannabifolius was observed by transmission electron microscopy. The results showed that the microspore mother cells were surrounded by callose; the cytokinesis was simultaneous; the tetrad was tetrahedral; and the mature pollen was two-celled pollen with three germination pores. During the meiosis of microspore mother cells, there were abundant organelles in the cytoplasm at leptotene stage. Obvious and clear synaptonemal complex was found in pachytene stage. In the prophase I stage, the number of organelles in the cytoplasm decreased; the cristae of mitochondria decreased; and the electron density of plastids was low. It began to recover at the metaphase I and formed an obvious organelle band in the anaphase I, which separated the two daughter nuclei and contained abundant organelles. During this period, the organelles in the cytoplasm changed regularly, which was the phenomenon of cytoplasmic reorganization. The pollen wall was formed at the late uninucleate stage; the pollen wall was mature at the binucleate microspore stage; and the pollen has abortion phenomenon. These results for the first time revealed the ultrastructure of microspores and male gametophytes during the development of S. cannabifolius and enriched the understanding of the formation of pollen grains by microspores in Compositae plants.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"1151-1161"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Desiccation tolerance in peatland desmids: a comparative study of Micrasterias thomasiana and Staurastrum hirsutum (Zygnematophyceae).","authors":"Y Nemcova, J Neustupa, M Pichrtová","doi":"10.1007/s00709-025-02061-1","DOIUrl":"10.1007/s00709-025-02061-1","url":null,"abstract":"<p><p>Desmids are valuable bioindicators in peatland ecosystems due to their sensitivity to environmental changes. In temperate and boreal wetlands, seasonal desiccation of aquatic habitats, which is increasing in frequency and severity due to ongoing climate change, is currently considered a key factor structuring the distribution of individual taxa. In this study, the desiccation tolerance of Micrasterias thomasiana and Staurastrum hirsutum isolated from contrasting hydrological environments in the peatland habitats of the Ore Mountains, Czech Republic, is investigated. Using controlled experimental conditions, we subjected both young, actively growing and old, mature cultures to four different desiccation treatments and evaluated morphology and photosynthetic performance. Our results showed that young and old cultures of both species exhibited a very similar photophysiological response. Severe desiccation led to an irreversible decline in the effective quantum yield of photosystem II in both species, resulting in cell death. Mild drought stress allowed the cultures to recover, indicating that the stress severity determines the recovery potential. Finally, prolonged desiccation resulted in irreversible damage in older cultures of both species, emphasizing the limited desiccation resilience of desmids. We observed similarities in morphology with Zygnema \"pre-akinetes,\" but in contrast to these resilient cells, the old cells of M. thomasiana and S. hirsutum did not survive the harsher desiccation conditions. Long-term mild desiccation revealed a higher resistance of S. hirsutum, probably due to the protective role of its dense mucilage. In nature, these two species usually inhabit localities with low desiccation risk or avoid and mitigate desiccation stress through localized survival strategies.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"1215-1228"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential cell wall composition in eriococcid-induced galls: unraveling the influence of sexual dimorphism.","authors":"Ana Flávia de Melo Silva, Gracielle Pereira Pimenta Bragança, Renato Dias Matosinhos, Luiza De Lazari Ferreira, Hállen Daniel Rezende Calado, Denis Coelho de Oliveira, Rosy Mary Dos Santos Isaias","doi":"10.1007/s00709-025-02065-x","DOIUrl":"10.1007/s00709-025-02065-x","url":null,"abstract":"<p><p>Eriococcid-induced galls exhibit many unusual features, such as sexual dimorphism and differences in life cycle duration, which are reflected in the morphology, anatomy, and metabolism of female- and male-induced galls. These sex-based distinctions between the galls result from differential developmental processes related to the time female and male insects remain inside the gall, their feeding activity, and the degree of stress imposed on the host plant cellular machinery. We assessed the immunocytochemical and Raman spectroscopy profiles of two host plant-gall inducer systems: Annona dolabripetala (Annonaceae)-Pseudotectococccus rolliniae Hodgson and Gonçalves, and Pseudobombax grandiflorum (Malvaceae)-Eriogallococcus isaias Hodgson and Magalhães. We expected differences in the dynamics of cell wall chemical components between male and female galls, with particular effects on gall structural and functional profiles, also regarding specific constraints in each system. The epitopes of xylogalacturonans and homogalacturonans, as well as their degree of methylesterification, are affected by the sex of the gall inducers, conferring a rigid structure to the cell walls of female-induced galls in comparison with those of the male-induced galls. Raman spectroscopy detected cellulose peaks in both female- and male-induced galls, with pronounced lignin bands in female-induced galls. The sex-based chemical distinctions between female- and male-induced galls imply in differences in terms of higher rigid cell walls in the female-induced galls, which was similar for both host plants.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"1259-1273"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerium oxide nanoparticles: biogenic synthesis, characterization, and effects of foliar application on photosynthetic and antioxidant performance on Brassica juncea L.","authors":"Mohammad Shiraz, Yamshi Arif, Havza Imtiaz, Ameer Azam, Pravej Alam, Shamsul Hayat","doi":"10.1007/s00709-025-02060-2","DOIUrl":"10.1007/s00709-025-02060-2","url":null,"abstract":"<p><p>The term \"green synthesis\" refers to the use of sustainable and environmentally friendly methods to produce materials, chemicals, or nanoparticles (NPs). This approach emphasizes the use of renewable resources, energy-efficient processes, and non-toxic chemicals to minimize environmental impact. In our study, we synthesized cerium oxide NPs (CeO₂ NPs) of varying crystal sizes using leaf extract from the Moringa oleifera plant and evaluated their effects on the photosynthetic and antioxidant properties of mustard (Brassica juncea L.). X-ray diffraction (XRD) analysis confirmed the successful synthesis of CeO₂ NPs, with average crystal sizes determined using the Debye-Scherrer equation as 4.5 nm, 8.5 nm, and 15.4 nm (designated as A, B, and C respectively). Fourier transform infrared spectroscopy (FTIR) analysis revealed stretching frequencies at 550 cm⁻¹, confirming the presence of Ce-O stretching bands and the use of natural compounds in the synthesis process. Scanning electron microscopy (SEM) analysis showed that the CeO₂ NPs were irregularly shaped and agglomerated, while transmission electron microscopy (TEM) analysis confirmed that the particles were spherical and polydisperse. Dynamic light scattering (DLS) and zeta potential analysis further confirmed the polydispersity and stability of synthesized NPs in solution. Following synthesis, the CeO₂ NPs were applied foliarly to mustard crops at concentrations of 50, 100, and 150 ppm. The results demonstrated that all concentrations of NPs enhanced growth, photosynthetic efficiency, and gaseous exchange parameters in mustard. Additionally, the NPs regulated balance between oxidation and reduction (redox) reactions in cell. It helps maintain cellular function by controlling reactive oxygen species (ROS) and antioxidants, preventing damage and ensuring normal metabolism. Notably, the 4.5 nm-sized NP (A) at a concentration of 100 ppm was the most effective in improving these parameters. CeO₂ NPs show promise as a sustainable alternative to traditional fertilizers and pesticides, contributing to more sustainable agricultural practices. This pioneering research highlights the potential of biogenically synthesized CeO₂ NPs in boosting crop performance, marking a significant advancement in agricultural nanotechnology.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"1229-1258"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical validation for the therapeutic use of Plumeria rubra in coagulation disorders: a study combining in silico, in vitro, and in vivo approaches.","authors":"Imran Ahmad Khan, Muhammad Anwar, Sarmad Frogh Arshad, Athar Hussain, Muhammad Usman, Mohammed Nadeem Ansari, Hasan Junaid Arshad, Asma Shah Rukh, Qurat Ul Ain, Maliha Khalid Khan","doi":"10.1007/s00709-025-02055-z","DOIUrl":"10.1007/s00709-025-02055-z","url":null,"abstract":"<p><p>Local healers in South Asia use Plumeria rubra Linn. leaves to treat various coagulation disorders in animals and humans. This study (in silico, in vitro, and in vivo) aimed to explore the pharmacological basis for the possible thrombolytic and anticlotting properties of the leaf extract of P. rubra. Phytoconstituents of P. rubra were dock against coagulation proteins: prothrombin, thromboplastin, and fibrin using in silico approach. Phytochemical screening, HPLC, and antioxidant, anticoagulant, and thrombolytic potential were evaluated using in vitro approach. Healthy male rabbits were divided into five groups (six rabbits each). Groups 1-3 were treated with aqueous-methanolic (30:70%) extract of P. rubra at 200, 300, and 600 mg/mL respectively groups in contrast to the positive and negative control groups. Thrombolytic activity was assessed at doses of 200, 300, and 600 µg/mL in comparison with standard urokinase (600 µg/kg). Platelet adhesion was evaluated at a dose of 200, 300, and 600 µg/mL against adrenaline (2 µM) and acute oral dose toxicity was assessed using in vivo approach. In silico study resulted in an excellent binding affinity and showed significant interaction with coagulation proteins. Phytochemical analysis showed a range of phytochemical classes: alkaloids, tannins, flavonoids, glycosides, anthraquinones, and saponins. HPLC analysis confirmed the phytoconstituents plumericin, rutin, kaempferol, and isoquercetin already reported for coagulation disorders. P. rubra showed excellent antioxidant potential and was assessed using DPPH, NO, and SOD assays. The activated partial thromboplastin time (APTT), bleeding time (BT), prothrombin time (PT), and clotting time (CT) all went up with increasing doses in the aqueous-methanolic extract (p ≤ 0.05). Comparing the plant extract to urokinase, the plant extract demonstrated considerable (p ≤ 0.05) clot lysis. Additionally, it dose-dependently delayed the ADR-induced platelet adhesion dose-dependently (p ≤ 0.05). The outcome of this study justifies its therapeutic utility in coagulation disorders and can be used as an alternative medicine.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"1319-1336"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoreactivity of flexible and inflexible scales in different lizards indicates that the mature beta-layer contains different corneous proteins.","authors":"Lorenzo Alibardi","doi":"10.1007/s00709-025-02048-y","DOIUrl":"10.1007/s00709-025-02048-y","url":null,"abstract":"<p><p>We have analyzed by immunohistochemical methods the Oberhautchen-beta layer, here abbreviated as beta-layer, of lizards with hard scales and lizards with softer scales. Different antibodies for detecting Corneous Beta Proteins (CBPs) have been utilized. The agamid beta-corneous layer is generally 3-4 times thicker than in geckos, and the surface microornamentation forms a honeycomb pattern. Geckos feature thinner beta-layers and a spinulated pattern and show immunolocalization for CBPs and isopeptide bonds, a product of transglutaminase catalysis, in their thin beta-layer. CBPs and isopeptide-bonds are detected in the superficial Oberhautchen and alpha-layer of agamids while the beta-layer is immuno-negative. Considering the limitations of immunolabeling methods, the unexpected result might derive from inaccessibility of antibodies to epitopes that are masked within the packed corneous material of the agamid beta-layer. However, bioinformatics analysis for CBPs sequenced in the few species of agamids so far known indicates that these proteins have low identity with those of other lizards. This suggests that agamids possess peculiar CBPs but low isopeptide bonds in their inflexible beta-layer. In contrast, the thinner and pliable beta-corneous layers of geckos may derive from the presence of isopeptide bonds mixed with lower amounts of CBPs. The differences in material properties of lizard scales, inflexibility versus pliability, are adaptive for the different ecological conditions of the species here analyzed.</p>","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"1129-1149"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}