Protoplasma最新文献_第2页

Osmotic priming-induced cryotolerance uncovers rejuvenation of grapevine cell cultures: morphogenetic changes and gene expression pattern highlighting enhanced embryogenic potential. 渗透引物诱导的低温耐受性揭示了葡萄细胞培养物的返老还童：形态发生变化和基因表达模式突显了胚胎生成潜力的增强。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-07-09 DOI: 10.1007/s00709-024-01968-5

Anis Ben-Amar, Dorsaf Allel, Badra Bouamama-Gzara

{"title":"Osmotic priming-induced cryotolerance uncovers rejuvenation of grapevine cell cultures: morphogenetic changes and gene expression pattern highlighting enhanced embryogenic potential.","authors":"Anis Ben-Amar, Dorsaf Allel, Badra Bouamama-Gzara","doi":"10.1007/s00709-024-01968-5","DOIUrl":"10.1007/s00709-024-01968-5","url":null,"abstract":"Cryopreservation is a reliable technique for the long-term storage and preservation of embryogenic cells, maintaining their viability without loss of their embryogenic capacity. However, the large-scale conservation of grapevine embryogenic lines in cryobanks remains limited. A significant challenge is understanding somatic cell rejuvenation. Here, we investigate the encapsulation/dehydration and encapsulation/vitrification for cryopreserving embryogenic material. Cell rejuvenation and enhanced embryogenic competence were observed after cryopreservation, as evidenced through structural cellular changes observed by histology and electron scanning microscopy. Results showed that cryopreserved samples of 110-Richter, Riesling, and Tempranillo using encapsulation/dehydration had better survival rates, averaging 81%, 62%, and 48%, respectively, while encapsulation/vitrification yielded lower survival rates, averaging 58%, 42%, and 32%, respectively. Cryopreservation also improved post-thaw recovery and regeneration efficiency assessed through regrowth of proembryogenic masses and somatic embryo conversion reaching 54-72% against 11-17% in control samples. Cryopreservation triggered changes in gene expression patterns and exhibited considerable increase at genotype-specific basis of 1.5- to 4.5-fold in SERK1, BBM, and WOX associated to embryogenic competence as well as in ChitIV and LEA involved in stress response. Membrane stability index, hydrogen peroxide, and proline contents were used as indicators of oxidative stress uncovering a key role of an osmotic trans-priming effect leading to cryotolerance. Our finding highlighted that cryopreservation enhances embryogenic capacity in senescent callus and probably acts as a screening process allowing safe maintenance of proembryogenic cells and promoting their recovery. This study provides a high throughput innovation to set up cryolines for cell rejuvenation of grapevine and other important plant species.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141559486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-specific calreticulin is localized in the nuclei of highly specialized cells in the pistil-new observations for an old hypothesis. 植物特异性钙调蛋白定位于雌蕊中高度特化细胞的细胞核中--一个古老假设的新观察结果。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-06-07 DOI: 10.1007/s00709-024-01961-y

Piotr Wasąg, Anna Suwińska, Anna Richert, Marta Lenartowska, Robert Lenartowski

{"title":"Plant-specific calreticulin is localized in the nuclei of highly specialized cells in the pistil-new observations for an old hypothesis.","authors":"Piotr Wasąg, Anna Suwińska, Anna Richert, Marta Lenartowska, Robert Lenartowski","doi":"10.1007/s00709-024-01961-y","DOIUrl":"10.1007/s00709-024-01961-y","url":null,"abstract":"One of the first cellular locations of the calreticulin (CRT) chaperone in eukaryotic cells, apart from its obvious localization in the endoplasmic reticulum (ER), was the cell nucleus (Opas et al. 1991). The presence of CRT has been detected inside the nucleus and in the nuclear envelope of animal and plant cells, and a putative nuclear localization signal (NLS) in the CRT amino acid sequence has been mapped in several animal and plant species. Over the last 30 years, other localization sites of this protein outside the ER and cell nucleus have also been discovered, suggesting that CRT is a multifunctional Ca2+-binding protein widely found in various cell types. In our previous studies focusing on plant developmental biology, we have demonstrated the presence of CRT inside and outside the ER in highly specialized plant cells, as well as the possibility of CRT localization in the cell nucleus. In this paper, we present a detailed analysis of immunocytochemical localization of CRT inside nuclei of the pistil transmission tract somatic cells before and after pollination. We show a similar pattern of the nuclear CRT localization in relation to exchangeable Ca2+ for two selected species of angiosperms, dicotyledonous Petunia and monocot Haemanthus, that differ in anatomical structure of the pistil and discuss the potential role of CRT in the cell nucleus.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the potential of microalgae for the production of monoclonal antibodies and other recombinant proteins. 利用微藻的潜力生产单克隆抗体和其他重组蛋白。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-07-06 DOI: 10.1007/s00709-024-01967-6

Balwinder Kaur Rajput, Sana Fatima Ikram, Bhumi Nath Tripathi

{"title":"Harnessing the potential of microalgae for the production of monoclonal antibodies and other recombinant proteins.","authors":"Balwinder Kaur Rajput, Sana Fatima Ikram, Bhumi Nath Tripathi","doi":"10.1007/s00709-024-01967-6","DOIUrl":"10.1007/s00709-024-01967-6","url":null,"abstract":"Monoclonal antibodies (mAbs) have become indispensable tools in various fields, from research to therapeutics, diagnostics, and industries. However, their production, primarily in mammalian cell culture systems, is cost-intensive and resource-demanding. Microalgae, diverse photosynthetic microorganisms, are gaining attention as a favorable option for manufacturing mAbs and various other recombinant proteins. This review explores the potential of microalgae as a robust expression system for biomanufacturing high-value proteins. It also highlights the diversity of microalgae species suitable for recombinant protein. Nuclear and chloroplast genomes of some microalgae have been engineered to express mAbs and other valuable proteins. Codon optimization, vector construction, and other genetic engineering techniques have significantly improved recombinant protein expression in microalgae. These accomplishments demonstrate the potential of microalgae for biopharmaceutical manufacturing. Microalgal biotechnology holds promise for revolutionizing the production of mAbs and other therapeutic proteins, offering a sustainable and cost-effective solution to address critical healthcare needs.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of Physalis alkekengi cell suspension culture: time-dependent behavior of genes related to the steroidal compounds, key enzymes, and physalins under static magnetic field. Physalis alkekengi 细胞悬浮培养的建立：在静态磁场下与甾体化合物、关键酶和物理肽有关的基因的时间依赖性行为。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-07-11 DOI: 10.1007/s00709-024-01966-7

Halimeh Hassanpour

{"title":"Establishment of Physalis alkekengi cell suspension culture: time-dependent behavior of genes related to the steroidal compounds, key enzymes, and physalins under static magnetic field.","authors":"Halimeh Hassanpour","doi":"10.1007/s00709-024-01966-7","DOIUrl":"10.1007/s00709-024-01966-7","url":null,"abstract":"Cell suspension culture has the potential to be a valuable source for the bioactive compound productions. In this study, an optimized procedure was established for callus and cell suspension culture of Physalis alkekengi for the first time, and the impact of static magnetic field (SMF, 6 mT) was studied on the high-value metabolic compounds through investigation of signaling molecules and gene expressions at the late log-to-stationary phase. Results showed that the growth regulators of 6-benzyl amino purine (BAP, 1.5 mg-1 L) and 1-naphthaleneacetic acid (NAA, 0.4 mg-1 L) induced the highest fresh weight, callus rate, callus index, and total withanolides. Cell suspension culture was established in the liquid MS medium supplied with BAP (1.5 mg-1 L) and NAA (0.1 mg-1 L). SMF application decreased slightly the cell growth and viability and enhanced the number of round-shaped cells. The hydrogen peroxide (H2O2) and nitric oxide (NO) levels increased at an all-time series after SMF exposure, and their maximum contents were observed after 12 h. A significant alteration of malondialdehyde content was also identified after 12 h of SMF exposure. The expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), 1-deoxyD-xylulose 5-phosphate synthase (DXS), squalene synthase (SQS), sterol Δ7-reductase (DWF5), and C-7,8 sterol isomerase (HYD1) genes was upregulated significantly after 24 and 48 h. An increase in the total withanolides was related to more activity of HMGR and DXS enzymes in SMF-exposed cells and the maximum physalin A (12.8 mg g-1 DW) and physalin B (1.92 mg g-1 DW) obtained after 24 h compared to controls. Findings suggest that SMF can play a supportive factor in inducing steroidal compounds in P. alkekengi through modulating H2O2 and NO levels and the related-gene expressions.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The function of the ATG8 in the cilia and cortical microtubule maintenance of Euplotes amieti. ATG8 在幼虫纤毛和皮层微管维持中的功能。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-05-20 DOI: 10.1007/s00709-024-01957-8

Junlin Wu, Yan Sheng, Shihuan Mai, Yanhao Zhong, Shengrong Dai, Yupeng Luo, Xin Sheng

引用次数: 0

Histochemical and ultrastructural analysis of tapetum and sporoderm development in relation to precocious pollenkitt production of Garcinia dulcis (Roxb.) Kurz. 组织化学和超微结构分析了与早熟花粉小体产生有关的革兰氏阴性体和孢子发育。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-07-16 DOI: 10.1007/s00709-024-01969-4

Pornsawan Sutthinon, Upatham Meesawat, Silvia Ulrich, Jürg Schönenberger

{"title":"Histochemical and ultrastructural analysis of tapetum and sporoderm development in relation to precocious pollenkitt production of Garcinia dulcis (Roxb.) Kurz.","authors":"Pornsawan Sutthinon, Upatham Meesawat, Silvia Ulrich, Jürg Schönenberger","doi":"10.1007/s00709-024-01969-4","DOIUrl":"10.1007/s00709-024-01969-4","url":null,"abstract":"Garcinia dulcis (Roxb.) Kurz (Clusiaceae) is a medicinal plant native to Southeastern Asia, with a peculiar, precocious pollenkitt production in early microspore development. We aimed to find out whether different secretory activities of the tapetum or a premature sporoderm development provides additional evidence for our recent hypothesis for the precocious pollenkitt production. Histology, histochemistry and ultrastructure of tapetum and sporoderm development during pollenkitt secretion in Garcinia dulcis were conducted, based on light and electron microscopy analysis. The results showed that Garcinia dulcis possesses normal pollen development. The presence of two different pollen coating types, precocious pollenkitt (L1) and common pollenkitt (L2), in the anther tapetum indicate that they are produced in two different active stages of the secretory tapetum. The precocious pollenkitt production and transport to the locule takes place in early active tapetal cells at early tetrad to early microspore stage and is ongoing until late microspore stage. The production of the second type of pollenkitt (L2) starts shortly after the first active tapetum stage together with the formation of sporopollenin precursors. The sporoderm formation was completed at late microspore stage, when the tapetal cell walls start to disintegrate. Orbicules are lining the inner tapetum wall at middle to late microspore stage. ER (during early microspore stage) and plastids (during late microspore stage) were the two main sources of pollenkitt, which finally fused to pollenkitt droplets when the tapetal cells degenerated at mature bicellular pollen stage.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141620787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen peroxide modulates the expression of the target of rapamycin (TOR) and cell division in Arabidopsis thaliana. 过氧化氢调节拟南芥中雷帕霉素靶标（TOR）的表达和细胞分裂。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-05-28 DOI: 10.1007/s00709-024-01959-6

Alma Alejandra Hernández-Esquivel, Jorge Alejandro Torres-Olmos, Manuel Méndez-Gómez, Elda Castro-Mercado, Idolina Flores-Cortéz, César Arturo Peña-Uribe, Jesús Campos-García, José López-Bucio, Homero Reyes-de la Cruz, Eduardo Valencia-Cantero, Ernesto García-Pineda

{"title":"Hydrogen peroxide modulates the expression of the target of rapamycin (TOR) and cell division in Arabidopsis thaliana.","authors":"Alma Alejandra Hernández-Esquivel, Jorge Alejandro Torres-Olmos, Manuel Méndez-Gómez, Elda Castro-Mercado, Idolina Flores-Cortéz, César Arturo Peña-Uribe, Jesús Campos-García, José López-Bucio, Homero Reyes-de la Cruz, Eduardo Valencia-Cantero, Ernesto García-Pineda","doi":"10.1007/s00709-024-01959-6","DOIUrl":"10.1007/s00709-024-01959-6","url":null,"abstract":"Hydrogen peroxide (H2O2) is naturally produced by plant cells during normal development and serves as a messenger that regulates cell metabolism. Despite its importance, the relationship between hydrogen peroxide and the target of rapamycin (TOR) pathway, as well as its impact on cell division, has been poorly analyzed. In this study, we explore the interaction of H2O2 with TOR, a serine/threonine protein kinase that plays a central role in controlling cell growth, size, and metabolism in Arabidopsis thaliana. By applying two concentrations of H2O2 exogenously (0.5 and 1 mM), we could correlate developmental traits, such as primary root growth, lateral root formation, and fresh weight, with the expression of the cell cycle gene CYCB1;1, as well as TOR expression. When assessing the expression of the ribosome biogenesis-related gene RPS27B, an increase of 94.34% was noted following exposure to 1 mM H2O2 treatment. This increase was suppressed by the TOR inhibitor torin 2. The elimination of H2O2 accumulation with ascorbic acid (AA) resulted in decreased cell division as well as TOR expression. The potential molecular mechanisms associated with the effects of H2O2 on the cell cycle and TOR expression in roots are discussed in the context of the results.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141156895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ABA inhibits in vitro shoot regeneration by affecting H3K9ac modification of WUS in Arabidopsis. ABA 通过影响拟南芥 WUS 的 H3K9ac 修饰抑制离体芽再生。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.1007/s00709-024-01984-5

Yuguang Song, Xinru Ding, Xueying Sun, Zhaoran Zhang, Wei Dong

引用次数: 0

Rosmarinus officinalis L. essential oil enhances salt stress tolerance of durum wheat seedlings through ROS detoxification and stimulation of antioxidant defense. 迷迭香精油通过 ROS 解毒和刺激抗氧化防御增强硬质小麦幼苗对盐胁迫的耐受性。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-11-01 Epub Date: 2024-06-28 DOI: 10.1007/s00709-024-01965-8

Rania Ben Saad, Walid Ben Romdhane, Alina Wiszniewska, Narjes Baazaoui, Mohamed Taieb Bouteraa, Yosra Chouaibi, Mohammad Y Alfaifi, Miroslava Kačániová, Natália Čmiková, Anis Ben Hsouna, Stefania Garzoli

{"title":"Rosmarinus officinalis L. essential oil enhances salt stress tolerance of durum wheat seedlings through ROS detoxification and stimulation of antioxidant defense.","authors":"Rania Ben Saad, Walid Ben Romdhane, Alina Wiszniewska, Narjes Baazaoui, Mohamed Taieb Bouteraa, Yosra Chouaibi, Mohammad Y Alfaifi, Miroslava Kačániová, Natália Čmiková, Anis Ben Hsouna, Stefania Garzoli","doi":"10.1007/s00709-024-01965-8","DOIUrl":"10.1007/s00709-024-01965-8","url":null,"abstract":"Salt-induced stress poses a significant barrier to agricultural productivity by impeding crop growth. Presently, environmentalists are dedicated to safeguarding food security by enhancing agricultural yields in challenging environments. Biostimulants play a crucial role in mitigating abiotic stresses in crop production, and among these, plant essential oils (EOs) stand out as organic substances with diverse biological effects on living organisms. Among the natural promoters of plant growth, Rosmarinus officinalis L. essential oil (RoEO) has gained considerable attention. Although the manifold effects of essential oils (EOs) on plant growth have been extensively demonstrated, their impact on salt stress tolerance in durum wheat seedlings remains unexplored. This investigation was undertaken to evaluate the biostimulatory capabilities of RoEO on the durum wheat cultivar \"Mahmoudi.\" The effects of three RoEO concentrations (1, 2.5, and 5 ppm) on seed germination, growth establishment, and the induction of salt resistance under salinity conditions (150 mM NaCl) were tested. At 5 ppm, RoEO enhanced seedlings' tolerance to salinity by improving growth and reducing membrane deterioration and oxidative stress-induced damage. The expression profile analyses of seven stress-related genes (TdNHX1, TdSOS1, TdSOD, TdCAT, TdGA20-ox1, TdNRT2.1, and TdGS) using RT-qPCR showed enhancement of several important genes in durum wheat seedlings treated with 5 ppm RoEO, even under control conditions, which may be related to salt stress tolerance. The results indicate that the application of RoEO suggests a possible alternative strategy to increase salt tolerance in durum wheat seedlings towards better growth quality, thus increasing ROS scavenging and activation of antioxidant defense.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting the role of β-GAL genes in bean under abiotic stress and genome-wide characterization of β-GAL gene family members. 预测β-GAL基因在非生物胁迫下对蚕豆的作用及β-GAL基因家族成员的全基因组特征。

IF 2.5 3区生物学

Protoplasma Pub Date : 2024-10-23 DOI: 10.1007/s00709-024-01998-z

Azize Buttanri, Ayşe Gül Kasapoğlu, Burak Muhammed Öner, Ahmed Sidar Aygören, Selman Muslu, Emre İlhan, Ertan Yildirim, Murat Aydin

{"title":"Predicting the role of β-GAL genes in bean under abiotic stress and genome-wide characterization of β-GAL gene family members.","authors":"Azize Buttanri, Ayşe Gül Kasapoğlu, Burak Muhammed Öner, Ahmed Sidar Aygören, Selman Muslu, Emre İlhan, Ertan Yildirim, Murat Aydin","doi":"10.1007/s00709-024-01998-z","DOIUrl":"https://doi.org/10.1007/s00709-024-01998-z","url":null,"abstract":"Β-Gals are a subgroup of the glycoside hydrolase (GH) family of enzymes, which possess the Glyco_hydro_35 (GH35) domain. Although studies have been conducted on the β-Gal gene family in numerous plant species, no such research has been conducted on beans. The purpose of this study was to determine the gene expression levels of β-Gal genes in the leaf tissue of P. vulgaris under salt and drought stress using quantitative real-time polymerase chain reaction (qRT-PCR) and to perform a comprehensive analysis of β-Gal gene family members using bioinformatics tools. In the bean genome, 25 Pvul-βGAL proteins with amino acid numbers ranging from 291 to 1119, molecular weights from 32.94 to 126.56 kDa, and isoelectric points from 5.46 to 9.08 were identified. Both segmental and tandem duplication have occurred in β-Gal genes in the bean genome, and Pvul-BGAL genes have been subject to negative selection in the evolutionary process. For a deeper comprehension of the evolutionary proximity of Pvul-BGAL genes, a phylogenetic tree and synteny map were drawn together with Arabidopsis thaliana and Glycine max β-Gal genes. The expression profiles of β-Gal genes in different tissues of the bean were determined in silico. In addition, the expression profiles of β-Gal genes in the leaves of bean plants subjected to drought and salt stress were analyzed, and the role of β-Gal genes in salt and drought stress was estimated. In this study, the role of β-Gal gene family in abiotic stress response and the characterization of β-Gal genes in beans were determined for the first time and will provide a basis for future functional genomics studies.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0