Protoplasma最新文献_第2页

Structural considerations and differences between leaf canals and secretory cavities in Asteraceae. 菊科植物叶管和分泌腔的结构考虑及其差异。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-14 DOI: 10.1007/s00709-024-02028-8

Daniel M Martínez-Quezada, Alicia Rojas-Leal, José Luis Villaseñor, Teresa Terrazas

{"title":"Structural considerations and differences between leaf canals and secretory cavities in Asteraceae.","authors":"Daniel M Martínez-Quezada, Alicia Rojas-Leal, José Luis Villaseñor, Teresa Terrazas","doi":"10.1007/s00709-024-02028-8","DOIUrl":"https://doi.org/10.1007/s00709-024-02028-8","url":null,"abstract":"Secretory canals are distributed among seed plants, and their diversity is concentrated in many families of angiosperms, while other internal secretory structures such as secretory cavities have been identified only in Rutaceae, Myrtaceae, and Asteraceae. Identifying and recognizing these two types of secretory structures has been complicated, mainly due to their structural similarities. In this study, the ontogeny of canals and secretory cavities in two species of Asteraceae are described and compared, to understand the structural differences between them and allow the establishment of more appropriate homology hypotheses. Leaves of Bidens odorata and Tagetes tenuifolia in different stages of development, including the apex of the stems, were collected. The samples were processed using the methacrylate technique, and longitudinal and transverse sections were made. The development of both, canals and secretory cavities, is schizogenous, in contrast to what was previously reported for other families such as Rutaceae, where they are reported as lysigenous. In Asteraceae, canals originate from cells of the procambium while cavities originate from cells of the ground meristem. The structural and developmental similarities between both types of secretory structures allow us to infer that they have a close evolutionary origin. Canals and secretory cavities in Asteraceae can be differentiated based on the number of strata of secretory epithelium and sheath, the modifications of epidermal cells and mesophyll, and the type of promeristem that gives rise to them. Probably extravascular canals give rise to cavities in leaves of Asteraceae and probably in other plant families.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New nucleolin-containing cytoplasmic bodies in an archamoebian protist Pelomyxa belevskii (Amoebozoa, Archamoebae, Pelobiontida). 古变形虫原生动物 Pelomyxa belevskii（变形虫纲、古变形虫目、天牛目）中新出现的含核素的细胞质体。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-13 DOI: 10.1007/s00709-024-02017-x

Dmitry S Bogolyubov, Ludmila V Chistyakova, Alexandra O Travina, Maksim I Sulatsky, Andrew V Goodkov

{"title":"New nucleolin-containing cytoplasmic bodies in an archamoebian protist Pelomyxa belevskii (Amoebozoa, Archamoebae, Pelobiontida).","authors":"Dmitry S Bogolyubov, Ludmila V Chistyakova, Alexandra O Travina, Maksim I Sulatsky, Andrew V Goodkov","doi":"10.1007/s00709-024-02017-x","DOIUrl":"https://doi.org/10.1007/s00709-024-02017-x","url":null,"abstract":"The representatives of the archamoebian genus Pelomyxa are amoeboid anaerobic protists that inhabit fresh-water anoxic sediments, and most of them are usually multinucleate. The cytoplasm of these unicellular organisms is highly complicated and contains numerous vacuoles of different types, as well as a wide range of prokaryotic endocytobionts, agglomerations of glycogen, lipids, etc. Among the great variety of cytoplasmic structures in P. belevskii, we identified novel organelles termed Cytoplasmic Nucleolin-Rich Bodies (CNRBs) due to their enrichment in nucleolin, a nuclear/nucleolar protein. The P. belevskii CNRBs differ significantly from known cytoplasmic nucleolin-related organelles encountered in some other eukaryotic cells, but their biological significance remains elusive. The work also provides the first description of the nuclear organization of P. belevskii. The nucleolar apparatus of P. belevskii contains little nucleolin, as determined by quantitative electron microscopic data, suggesting that it is inactive despite its morphological complexity. The presence of CNRBs in Pelomyxa is discussed in the context of the specific habitat conditions and biology of these unicellular eukaryotes.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vindoline is a key component of Catharanthus roseus leaf juice extract prepared through an Ayurveda-based method for ameliorating insulin-resistant type 2 diabetes. 长春花碱是一种关键成分的玫瑰花叶提取物制备通过阿育吠陀为基础的方法，改善胰岛素抵抗型2型糖尿病。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-11 DOI: 10.1007/s00709-024-02026-w

Pooja Singh, Dewasya P Singh, Manish K Patel, Monika Binwal, Amit Kaushik, Maneesha Mall, Mridula Sahu, Puja Khare, Karuna Shanker, Dnyaneshwar U Bawankule, Velusamy Sundaresan, Daya N Mani, Ashutosh K Shukla

{"title":"Vindoline is a key component of Catharanthus roseus leaf juice extract prepared through an Ayurveda-based method for ameliorating insulin-resistant type 2 diabetes.","authors":"Pooja Singh, Dewasya P Singh, Manish K Patel, Monika Binwal, Amit Kaushik, Maneesha Mall, Mridula Sahu, Puja Khare, Karuna Shanker, Dnyaneshwar U Bawankule, Velusamy Sundaresan, Daya N Mani, Ashutosh K Shukla","doi":"10.1007/s00709-024-02026-w","DOIUrl":"https://doi.org/10.1007/s00709-024-02026-w","url":null,"abstract":"Catharanthus roseus leaves have been traditionally described to possess potent antidiabetic activity and some leaf-specific alkaloids, including vindoline, have been studied for their antidiabetic potential. The aim of the present study was to validate the antidiabetic property of the plant with special reference to vindoline. An Ayurveda-based method was used to prepare the Swaras [leaf juice extract (LJE)] of three familial C. roseus genotypes differing in their vindoline content [CIM-Sushil (CS) > Dhawal (D) > Nirmal (N)]. In vivo experiments using LJE were performed in Charles Foster rats, whereby metformin (M100, 100 mg/kg BW) and vindoline (V20, 20 mg/kg BW) were used for comparison. OGTT-based screening for LJE doses (N100, N300, N500, D100, D200, D300, CS100, CS200, CS300 mg/kg BW) was carried out. Further analysis of the effective doses (D100, D200, D300, CS100, CS200, CS300) in streptozotocin-induced diabetic rats indicated highest blood glucose depletion in D300 (52.51%) and CS200 (64.55%) together with V20 (56.96%) on the 14th day. CS-LJE was found to be safe up to 2000 mg/kg BW. The role of LJE/vindoline in maintaining glucose homeostasis in liver was found to be mediated through the expression of insulin pathway genes (IRS-1, PI3K, AKT, GLUT2). TNF-α-induced insulin resistance in L6 skeletal muscle cells was used to analyze the effect of LJE/vindoline through glucose uptake assay and expression analysis of insulin pathway genes (IRS-1, PI3K, AKT, GLUT4). The results indicated that the antidiabetic effect of LJE/vindoline is mediated through activation of IRS/PI3K/AKT/GLUT signaling pathway.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The anatomy and histology of the midgut and Malpighian tubules of Copris felschei Reitter, 1892 (Coleoptera: Scarabaeidae). 金龟甲科金龟甲的中肠和马尔匹氏小管的解剖和组织学。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-11 DOI: 10.1007/s00709-024-02021-1

Nurcan Özyurt Koçakoğlu, Doğan Erhan Ersoy, Hicret Arslan, Selami Candan

{"title":"The anatomy and histology of the midgut and Malpighian tubules of Copris felschei Reitter, 1892 (Coleoptera: Scarabaeidae).","authors":"Nurcan Özyurt Koçakoğlu, Doğan Erhan Ersoy, Hicret Arslan, Selami Candan","doi":"10.1007/s00709-024-02021-1","DOIUrl":"https://doi.org/10.1007/s00709-024-02021-1","url":null,"abstract":"Copris are part of the Scarabaeidae family of Coleoptera. Copris are dung beetles or coprophagous beetles. These insects are called tunnelers because they excavate channels in the substrate. They use dead organisms and non-living organic compounds as a nutrient source. By breaking down dead matter, they provide nutrients that are important to the environment and necessary for the survival of other organisms. No studies have yet examined the midgut structure and Malpighian tubules of Copris. Therefore, this study investigated the histo-anatomical structure of the midgut and Malpighian tubules of Copris felschei Reitter, 1892 (Coleoptera: Scarabaeidae) using light and scanning electron microscopy (SEM) in detail. The midgut of C. felschei represents the largest part of the alimentary canal. Muscle layers and a monolayer of cylindrical epithelium surround the midgut wall. A peritrophic membrane envelops food in the midgut lumen, and crystals were observed within the lumen. The surface of the midgut has regenerative crypts and tracheae. The Malpighian tubules are arranged in two pairs and connect proximally between the midgut and hindgut. The Malpighian tubules are composed of a single layer of cuboidal epithelium. Numerous balloon-like tracheae were observed interspersed between the midgut and Malpighian tubules. Light and SEM images of the tracheae reveal a spongy structure with hollow chambers. These findings are anticipated to advance future research and deepen our understanding of the alimentary canal in Coleoptera, particularly within the Scarabaeidae family.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytohormonal homeostasis, chloroplast stability, and heat shock transcription pathways related to the adaptability of creeping bentgrass species to heat stress. 匍匐弯草对热胁迫的适应性与植物激素稳态、叶绿体稳定性和热休克转录途径相关。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-10 DOI: 10.1007/s00709-024-02022-0

Huizhen Yang, Yan Yuan, Xinying Liu, Yong Du, Zhou Li

{"title":"Phytohormonal homeostasis, chloroplast stability, and heat shock transcription pathways related to the adaptability of creeping bentgrass species to heat stress.","authors":"Huizhen Yang, Yan Yuan, Xinying Liu, Yong Du, Zhou Li","doi":"10.1007/s00709-024-02022-0","DOIUrl":"https://doi.org/10.1007/s00709-024-02022-0","url":null,"abstract":"Creeping bentgrass (Agrostis stolonifera) is a cool-season perennial turfgrass and is frequently utilized in high-quality turf areas. However, a poor to moderate resistance to heat stress limits its promotion and utilization in transitional and worm climate zones. The objectives of the study were to assess the heat tolerance of 18 creeping bentgrass genotypes in the field and to further uncover differential mechanisms of heat tolerance between heat-tolerant and heat-sensitive genotypes. The results showed that 18 different genotypes had different heat tolerance during summer months of 2021 and 2022. Among them, 13 M was identified as the best heat-tolerant cultivar based on the subordinate function values analysis of five physiological indicators. Under controlled growth conditions, heat stress significantly inhibited photosynthetic capacity and also accelerated oxidative damage and chlorophyll (Chl) degradation in both heat-tolerant 13 M and heat-sensitive PA4. However, as compared with heat-sensitive PA4, 13 M maintained significantly higher net photosynthetic rate, water use efficiency, and total antioxidant capacity as well as less Chl degradation and damage to chloroplast ultrastructure. Significantly higher contents of abscisic acid, cytokinin, gibberellin, and polyamines (spermine, spermidine, and putrescine) were also detected in 13 M than that in PA4 in the later stage of heat stress, but 13 M exhibited significantly lower indoleacetic acid content than PA4 during heat stress. In addition, heat-upregulated genes involved in heat shock transcriptional pathways were more pronounced in 13 M than in PA4. These findings indicated that better heat tolerance of 13 M could be related to more stable Chl metabolism, better photosynthetic and antioxidant capacities, endogenous hormonal homeostasis, and more effective heat shock transcriptional pathway. 13 M is more appropriate for planting in transitional and subtropical zones instead of widely used PA4.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SQSTM1/p62 predicts prognosis and upregulates the transcription of CCND1 to promote proliferation in mantle cell lymphoma. SQSTM1/p62在套细胞淋巴瘤中预测预后并上调CCND1的转录促进增殖。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-09 DOI: 10.1007/s00709-024-02023-z

Shuxia Zhang, Feichao Huang, Jin Wang, Ruolan You, Qiqi Huang, Yuanzhong Chen

{"title":"SQSTM1/p62 predicts prognosis and upregulates the transcription of CCND1 to promote proliferation in mantle cell lymphoma.","authors":"Shuxia Zhang, Feichao Huang, Jin Wang, Ruolan You, Qiqi Huang, Yuanzhong Chen","doi":"10.1007/s00709-024-02023-z","DOIUrl":"https://doi.org/10.1007/s00709-024-02023-z","url":null,"abstract":"Mantle cell lymphoma (MCL) is a rare, highly invasive non-Hodgkin's lymphoma. The main pathogenesis of MCL is associated with the formation of the IgH/CCND1 fusion gene and nuclear overexpression of cyclin D1, which accelerates the cell cycle, leading to tumorigenesis. The prognosis with current standard chemotherapy is still unsatisfactory. SQSTM1/p62 is a multifunctional adaptor that plays an important role in various tumors. Here, we found that the expression of p62 in MCL tissues was higher than that in hyperplastic lymphadenitis patients. Patients with low p62 expression in MCL cells had better overall survival and progression-free survival rates than those with high expression (p = 0.024 and p = 0.025, respectively). Multivariate Cox analysis indicated that the calculated death risk (hazard ratio [HR]) in patients with high expression levels of p62 increased to 2.742 (95% confidence interval (CI) of 1.268-5.852, p = 0.01), which was higher than those with low levels. Silencing p62 impaired Jeko-1 and Granta519 cell proliferation while downregulating CCND1 mRNA and protein expression, thereby inducing G0/G1 cell cycle arrest. However, silencing p62 does not affect the fusion of IgH and CCND1. Luciferase reporter gene analysis and chromatin immunoprecipitation analysis demonstrated that p62 may regulate CCND1 gene expression through Nrf2. These results provide evidence that p62 can predict poor prognosis in MCL. The precise targeting of p62 therapy reduces the promoting effect of Nrf2 on CCND1, thereby preventing cell cycle progression and effectively inhibiting tumor proliferation. Therefore, p62 may provide a potential target for MCL.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methyl jasmonate induces the regulation of protostane triterpene biosynthesis by microRNAs in Alisma orientale. 茉莉酸甲酯诱导泽泻原烷三萜合成的microrna调控。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-08 DOI: 10.1007/s00709-024-02029-7

Wenyuan Run, Tao Li, Shengyuan Wang, Shan Xiao, YuHeng Wu, Wei Gu

{"title":"Methyl jasmonate induces the regulation of protostane triterpene biosynthesis by microRNAs in Alisma orientale.","authors":"Wenyuan Run, Tao Li, Shengyuan Wang, Shan Xiao, YuHeng Wu, Wei Gu","doi":"10.1007/s00709-024-02029-7","DOIUrl":"https://doi.org/10.1007/s00709-024-02029-7","url":null,"abstract":"Protostane triterpenes are medicinally important components found in members of the Alismataceae botanical family, notably Alisma orientale. Methyl jasmonate (MeJA) is known to regulate protostane triterpene biosynthesis in A. orientale, but the microRNA (miRNA) mechanism underlying MeJA response to promote triterpene biosynthesis remains unknown. In this study, we conducted miRNA sequencing analysis after MeJA induction in A. orientale to uncover the role of miRNAs in protostane triterpene biosynthesis. We identified 222 known miRNAs and 379 novel miRNAs, including 16 differentially expressed miRNAs (DEMs) between control and MeJA-treated leaf samples. Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) enrichment analysis, four DEMs and eight miRNA target genes were significantly enriched in the triterpene biosynthesis pathway. Integrated analysis of the transcriptome and miRNAome revealed a negative expression pattern between miRNAs and their target genes. We then constructed a regulatory network of miRNA-target gene relationships involved in the triterpene biosynthesis pathway. We found miRNAs may be involved in the response of A. orientale to exogenous MeJA by regulating the expression of key biosynthesis enzymes, leading to increased accumulation of medically important protostane triterpenes.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antennal sensilla variability among castes and sexes in the leaf-cutter ant Acromyrmex subterraneus subterraneus. 切叶蚁触角感受器在种姓和性别间的变异。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-04 DOI: 10.1007/s00709-024-02027-9

Weslane Silva Noronha, Renan Dos Santos Araújo, Gustavo Ferreira Martins

{"title":"Antennal sensilla variability among castes and sexes in the leaf-cutter ant Acromyrmex subterraneus subterraneus.","authors":"Weslane Silva Noronha, Renan Dos Santos Araújo, Gustavo Ferreira Martins","doi":"10.1007/s00709-024-02027-9","DOIUrl":"https://doi.org/10.1007/s00709-024-02027-9","url":null,"abstract":"Insect antennae play a crucial role in communication, acting as receptors for both chemical and physical cues. This sensory reception is facilitated by specialized cuticular structures known as sensilla, which exhibit diverse morphologies and functions. In ants, caste polymorphism and sexual dimorphism manifest in antennal structure. This study characterized and compared the antennal sensilla across different castes (major and minor workers, and queens) and sexes of the leaf-cutter ant Acromyrmex subterraneus subterraneus, an important agricultural pest, using scanning electron microscopy. The lengths of the scape and pedicel were greater in major workers and queens compared to other classes of individuals (i.e., minor workers and males). Sensillum numbers were significantly higher for these two female classes than for minor workers and tended to be higher than for males. In addition, various types of sensilla were identified, including chaetic types I, II, and III, trichoid I and II, basiconic, ampullaceous, and coeloconic. Chaetic sensilla were found to be the most abundant in all classes of individuals studied. Overall, similar types of sensilla were found in females (major, minor, queen) and males. The differences in the presence, absence, and number of antennal sensilla were analyzed in relation to their functional roles across the studied groups.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhodotorula mucilaginosa: a new potential human pathogen found in the ciliate Paramecium bursaria. 粘液红曲菌：在囊草履虫中发现的一种新的潜在人类病原体。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-02 DOI: 10.1007/s00709-024-02025-x

Elena Sabaneyeva, Ekaterina Kursacheva, Galina Vizichkanich, Dmitrii Lebedev, Natalia Lebedeva

{"title":"Rhodotorula mucilaginosa: a new potential human pathogen found in the ciliate Paramecium bursaria.","authors":"Elena Sabaneyeva, Ekaterina Kursacheva, Galina Vizichkanich, Dmitrii Lebedev, Natalia Lebedeva","doi":"10.1007/s00709-024-02025-x","DOIUrl":"https://doi.org/10.1007/s00709-024-02025-x","url":null,"abstract":"Ciliates often form symbiotic associations with other microorganisms, both prokaryotic and eukaryotic. We are now starting to rediscover the symbiotic systems recorded before molecular analysis became available. Here, we provide a morphological and molecular characterization of a symbiotic association between the ciliate Paramecium tritobursaria and the yeast Rhodotorula mucilaginosa (syn. Rhodotorula rubra) isolated from a natural population. This symbiotic system demonstrates certain similarities with the symbiotic system formed by P. bursaria and its conventional endosymbionts, the zoochlorellae. Experimental infections of the endosymbiont-free P. tritobursaria and Paramecium deuterobursaria cell lines with R. mucilaginosa demonstrated that the yeast infectivity is concentration-dependent, with ciliates digesting part of the yeast cells. The endosymbiotic yeast may serve as a food reserve, providing starvation stress tolerance to the host. Since R. mucilaginosa is currently regarded as a pathogen causing opportunistic infections in immunocompromised humans, our finding gives further support to the vision that ciliates can harbor potential human pathogens and can be a vector for their dissemination.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemal sinus basal laminae contact sites: a possible route between gonadal lumen and myoepithelial cells in the gonad of the sea star Patiria pectinifera. 半月窦基底薄片接触点：海星帕蒂瑞亚（Patiria pectinifera）性腺内腔与肌上皮细胞之间的可能通道。

IF 2.5 3区生物学

Protoplasma Pub Date : 2025-01-01 Epub Date: 2024-08-14 DOI: 10.1007/s00709-024-01979-2

Uliana E Shulga, Alexander V Kalachev

{"title":"Hemal sinus basal laminae contact sites: a possible route between gonadal lumen and myoepithelial cells in the gonad of the sea star Patiria pectinifera.","authors":"Uliana E Shulga, Alexander V Kalachev","doi":"10.1007/s00709-024-01979-2","DOIUrl":"10.1007/s00709-024-01979-2","url":null,"abstract":"Sea stars are a group of marine invertebrates suitable for studying the hormonal regulation of reproduction and spawning. In spite of substantial progress in understanding how various substances such as 1-methyladenine act in their gonads, there are still many gaps concerning the fine details of their action. One such gap is how the gonadal wall contraction is induced. Recent literature data suggest that, upon 1-methyladenine stimulation, some cells within the gonadal lumen produce non-neuronal acetylcholine that, upon contact with the gonadal wall, induces contraction of myoepithelial cells. Our ultrastructural study of the gonads in the sea star Patiria pectinifera has shown, for the first time, that there are sites where the basal laminae bordering the hemal sinus directly contact one another and appear at this contact site as a single entity. These contact sites are often associated with hemidesmosome-like junctions that anchor male accessory cells or female follicle cells on one side of the site and myoepithelial cells on the opposite. We suggest that contraction-inducing substance is secreted from an accessory or follicle cell, passes through a basal lamina contact site, and on the opposite side of the contact site acts on a myoepithelial cell to induce its contraction.","PeriodicalId":20731,"journal":{"name":"Protoplasma","volume":" ","pages":"61-71"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0