不同蜥蜴柔性鳞片和非柔性鳞片的免疫反应表明,成熟的β层含有不同的角蛋白。

IF 2.5 3区 生物学 Q3 CELL BIOLOGY
Lorenzo Alibardi
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引用次数: 0

摘要

我们通过免疫组化方法分析了硬鳞蜥和软鳞蜥的奥伯豪森-β层(在此简称为β层)。我们使用了不同的抗体来检测角膜β蛋白(CBPs)。姬蛙的β-角质层一般比壁虎厚 3-4 倍,表面微丝形成蜂窝状图案。壁虎的β角质层较薄,表面呈纺锤形图案,在薄的β角质层中显示出 CBPs 和转谷氨酰胺酶催化产物异肽键的免疫定位。CBPs 和异肽键在琼脂糖的表层 Oberhautchen 和 alpha 层被检测到,而 beta 层则呈免疫阴性。考虑到免疫标记方法的局限性,这一意想不到的结果可能是由于抗体无法接触到被掩盖在琼脂糖β层角质层中的表位。然而,对目前已知的少数几种姬蛙的 CBPs 测序进行的生物信息学分析表明,这些蛋白质与其他蜥蜴的蛋白质相似度很低。这表明,姬蛙具有特殊的 CBPs,但其不灵活的 beta 层中的异肽键较低。与此相反,壁虎的β角质层较薄且柔韧,这可能是由于异肽键与较低量的 CBP 混合在一起的缘故。蜥蜴鳞片材料特性的差异,即不柔韧与柔韧,是为了适应本文分析的物种的不同生态条件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunoreactivity of flexible and inflexible scales in different lizards indicates that the mature beta-layer contains different corneous proteins.

We have analyzed by immunohistochemical methods the Oberhautchen-beta layer, here abbreviated as beta-layer, of lizards with hard scales and lizards with softer scales. Different antibodies for detecting Corneous Beta Proteins (CBPs) have been utilized. The agamid beta-corneous layer is generally 3-4 times thicker than in geckos, and the surface microornamentation forms a honeycomb pattern. Geckos feature thinner beta-layers and a spinulated pattern and show immunolocalization for CBPs and isopeptide bonds, a product of transglutaminase catalysis, in their thin beta-layer. CBPs and isopeptide-bonds are detected in the superficial Oberhautchen and alpha-layer of agamids while the beta-layer is immuno-negative. Considering the limitations of immunolabeling methods, the unexpected result might derive from inaccessibility of antibodies to epitopes that are masked within the packed corneous material of the agamid beta-layer. However, bioinformatics analysis for CBPs sequenced in the few species of agamids so far known indicates that these proteins have low identity with those of other lizards. This suggests that agamids possess peculiar CBPs but low isopeptide bonds in their inflexible beta-layer. In contrast, the thinner and pliable beta-corneous layers of geckos may derive from the presence of isopeptide bonds mixed with lower amounts of CBPs. The differences in material properties of lizard scales, inflexibility versus pliability, are adaptive for the different ecological conditions of the species here analyzed.

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来源期刊
Protoplasma
Protoplasma 生物-细胞生物学
CiteScore
6.60
自引率
6.90%
发文量
99
审稿时长
4-8 weeks
期刊介绍: Protoplasma publishes original papers, short communications and review articles which are of interest to cell biology in all its scientific and applied aspects. We seek contributions dealing with plants and animals but also prokaryotes, protists and fungi, from the following fields: cell biology of both single and multicellular organisms molecular cytology the cell cycle membrane biology including biogenesis, dynamics, energetics and electrophysiology inter- and intracellular transport the cytoskeleton organelles experimental and quantitative ultrastructure cyto- and histochemistry Further, conceptual contributions such as new models or discoveries at the cutting edge of cell biology research will be published under the headings "New Ideas in Cell Biology".
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