Yuting Niu, Shuang He, Lu Xing, Dongdong Zhang, Yuze Li, Chong Deng, Yi Jiang, Huawei Zhang, Xiaomei Song, Meihai Ding, Wenli Huang, Wei Wang
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引用次数: 0
Abstract
To investigate the cadmium (Cd) uptake mechanism in the roots of Coptis chinensis Franch., we utilized non-invasive micro-test technology (NMT) to analyze Cd2+ and H+ fluxes, alongside ICP-MS to assess Cd content and fluxes under various inhibitors and ion channel blockers. Results revealed that Cd2+ uptake primarily occurs in the root meristematic zone. Cd2+ addition significantly inhibited H+ influx. The P-type ATPase inhibitor Na3VO4 and the metabolism inhibitor CCCP both reduced Cd2+ fluxes and Cd content in roots of C. chinensis Ca2+ channel blocker LaCl3 exhibited more pronounced inhibition on Cd2+ flux than K+ channel blocker TEA, indicating Cd2+ uptake mainly via Ca2+ channels. Therefore, active transport and Ca2+ channels may play an important role in Cd uptake in C. chinensis roots.
期刊介绍:
Protoplasma publishes original papers, short communications and review articles which are of interest to cell biology in all its scientific and applied aspects. We seek contributions dealing with plants and animals but also prokaryotes, protists and fungi, from the following fields:
cell biology of both single and multicellular organisms
molecular cytology
the cell cycle
membrane biology including biogenesis, dynamics, energetics and electrophysiology
inter- and intracellular transport
the cytoskeleton
organelles
experimental and quantitative ultrastructure
cyto- and histochemistry
Further, conceptual contributions such as new models or discoveries at the cutting edge of cell biology research will be published under the headings "New Ideas in Cell Biology".