Plant Biotechnology最新文献_第4页

Integrated gene-free potato genome editing using transient transcription activator-like effector nucleases and regeneration-promoting gene expression by <i>Agrobacterium</i> infection 利用瞬时转录激活子样效应核酸酶和农杆菌表达再生促进基因的整合马铃薯无基因基因组编辑感染

4区生物学

Plant Biotechnology Pub Date : 2023-09-25 DOI: 10.5511/plantbiotechnology.23.0530a

Naoyuki Umemoto, Shuhei Yasumoto, Muneo Yamazaki, Kenji Asano, Kotaro Akai, Hyoung Jae Lee, Ryota Akiyama, Masaharu Mizutani, Yozo Nagira, Kazuki Saito, Toshiya Muranaka

{"title":"Integrated gene-free potato genome editing using transient transcription activator-like effector nucleases and regeneration-promoting gene expression by <i>Agrobacterium</i> infection","authors":"Naoyuki Umemoto, Shuhei Yasumoto, Muneo Yamazaki, Kenji Asano, Kotaro Akai, Hyoung Jae Lee, Ryota Akiyama, Masaharu Mizutani, Yozo Nagira, Kazuki Saito, Toshiya Muranaka","doi":"10.5511/plantbiotechnology.23.0530a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.23.0530a","url":null,"abstract":"Genome editing is highly useful for crop improvement. The method of expressing genome-editing enzymes using a transient expression system in Agrobacterium, called agrobacterial mutagenesis, is a shortcut used in genome-editing technology to improve elite varieties of vegetatively propagated crops, including potato. However, with this method, edited individuals cannot be selected. The transient expression of regeneration-promoting genes can result in shoot regeneration from plantlets, while the constitutive expression of most regeneration-promoting genes does not result in normally regenerated shoots. Here, we report that we could obtain genome-edited potatoes by positive selection. These regenerated shoots were obtained via a method that combined a regeneration-promoting gene with the transient expression of a genome-editing enzyme gene. Moreover, we confirmed that the genome-edited potatoes obtained using this method did not contain the sequence of the binary vector used in Agrobacterium. Our data have been submitted to the Japanese regulatory authority, the Ministry of Education, Culture, Sports, Science and Technology (MEXT), and we are in the process of conducting field tests for further research on these potatoes. Our work presents a powerful method for regarding regeneration and acquisition of genome-edited crops through transient expression of regeneration-promoting gene.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135768730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Preface to the special issue “Current Status and Future Prospects for the Development of Crop Varieties and Breeding Materials Using Genome Editing Technology” 专刊《利用基因组编辑技术开发作物品种和选育材料的现状与展望》前言

4区生物学

Plant Biotechnology Pub Date : 2023-09-25 DOI: 10.5511/plantbiotechnology.23.0000p

Masahiro Nishihara, Toshiya Muranaka

引用次数: 0

Gene-flow investigation between garden and wild roses planted in close distance 近距离种植的花园玫瑰与野生玫瑰的基因流动研究

4区生物学

Plant Biotechnology Pub Date : 2023-01-01 DOI: 10.5511/plantbiotechnology.23.0708a

Yuna Asagoshi, Eri Hitomi, Noriko Nakamura, Seiji Takeda

{"title":"Gene-flow investigation between garden and wild roses planted in close distance","authors":"Yuna Asagoshi, Eri Hitomi, Noriko Nakamura, Seiji Takeda","doi":"10.5511/plantbiotechnology.23.0708a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.23.0708a","url":null,"abstract":"Rose is a major ornamental plant, and a lot of cultivars with attractive morphology, color and scent have been generated by classical breeding. Recent progress of genetic modification produces a novel cultivar with attractive features. In both cases, a major problem is the gene-flow from cultivated or genetically modified (GM) plants to wild species, causing reduction of natural population. To investigate whether gene-flow occurs in wild species, molecular analysis with DNA markers with higher efficient technique is useful. Here we investigated the gene-flow from cultivated roses (Rosa×hybrida) to wild rose species planted in close distance in the field. The overlapping flowering periods and visiting insects suggest that pollens were transported by insects between wild and cultivated roses. We examined the germination ratio of seeds from wild species, and extracted DNA and checked with KSN and APETALA2 (AP2) DNA markers to detect transposon insertions. Using two markers, we successfully detected the outcross between wild and cultivated roses. For higher efficiency, we established a bulking method, where DNA, leaves or embryos were pooled, enabling us to that check the outcross of many plants. Our results suggest that wild species and garden cultivars can cross in close distance, so that they should be planted in distance, and checked the outcross with multiple DNA markers.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135402370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway. 4-苯基丁酸作为一种生长素，通过ibr3独立通路转化为苯乙酸，促进植物再生。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2022-03-25 DOI: 10.5511/plantbiotechnology.21.1224b

Akira Iwase, Arika Takebayashi, Yuki Aoi, David S. Favero, Shunsuke Watanabe, M. Seo, Hiroyuki Kasahara, K. Sugimoto

{"title":"4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway.","authors":"Akira Iwase, Arika Takebayashi, Yuki Aoi, David S. Favero, Shunsuke Watanabe, M. Seo, Hiroyuki Kasahara, K. Sugimoto","doi":"10.5511/plantbiotechnology.21.1224b","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.21.1224b","url":null,"abstract":"4-Phenylbutyric acid (4PBA) is utilized as a drug to treat urea cycle disorders and is also being studied as a potential anticancer drug that acts via its histone deacetylase (HDAC) inhibitor activity. During a search to find small molecules that affect plant regeneration in Arabidopsis, we found that 4PBA treatment promotes this process by mimicking the effect of exogenous auxin. Specifically, plant tissue culture experiments revealed that a medium containing 4PBA enhances callus formation and subsequent shoot regeneration. Analyses with auxin-responsive or cytokinin-responsive marker lines demonstrated that 4PBA specifically enhances AUXIN RESPONSE FACTOR (ARF)-dependent auxin responses. Our western blot analyses showed that 4PBA treatment does not enhance histone acetylation in Arabidopsis, in contrast to butyric acid and trichostatin A, other chemicals often used as HDAC inhibitors, suggesting this mechanism of action does not explain the observed effect of 4PBA on regeneration. Finally, mass spectroscopic analysis and genetic approaches uncovered that 4PBA in Arabidopsis plants is converted to phenylacetic acid (PAA), a known natural auxin, in a manner independent of peroxisomal IBR3-related β-oxidation. This study demonstrates that 4PBA application promotes regeneration in explants via its auxin activity and has potential applications to not only plant tissue culture engineering but also research on the plant β-oxidation pathway.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"39 1 1","pages":"51-58"},"PeriodicalIF":1.6,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41547470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Near-Isogenic Lines as Powerful Tools to Evaluate the Effect of Individual Phytochemicals on Health and Chronic Diseases 近等基因系作为评估单个植物化学物质对健康和慢性疾病影响的有力工具

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2021-01-01 DOI: 10.1007/978-3-030-68345-0_17

Binning Wu, Jairam K. P. Vanamala, S. Chopra, L. Reddivari

引用次数: 1

New Technologies for Precision Plant Breeding 植物精密育种新技术

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2021-01-01 DOI: 10.1007/978-3-030-68345-0_4

Shdema Filler-Hayut, C. Melamed-Bessudo, A. Levy

引用次数: 0

Production of Medicines from Engineered Proteins in Plants 利用植物工程蛋白生产药物