Plant Biotechnology最新文献_第4页

Synthetic-biology approach for plant lignocellulose engineering.

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-09-25 DOI: 10.5511/plantbiotechnology.24.0630a

Kouki Yoshida, Shingo Sakamoto, Nobutaka Mitsuda

{"title":"Synthetic-biology approach for plant lignocellulose engineering.","authors":"Kouki Yoshida, Shingo Sakamoto, Nobutaka Mitsuda","doi":"10.5511/plantbiotechnology.24.0630a","DOIUrl":"10.5511/plantbiotechnology.24.0630a","url":null,"abstract":"Plant biomass is an abundant, renewable resource that offers multiple advantages for the production of green chemicals and recombinant proteins. However, the adoption of plant-based systems by industry is hindered because mammalian and other cell cultures are well-established and better characterized in an industrial setting, and thus it is difficult for plant-based processes to gain a foothold in the marketplace. Therefore, additional benefits of plant-based systems may be essential to tip the balance in favor of sustainable plant-derived products. A crucial factor in biomass valorization is to design mid- to high-value co-products that can be derived cost-effectively from the residual lignocellulose (LC). However, the utility of LC remains limited because LCs are, in general, too recalcitrant for industries to utilize their components (lignin, cellulose, and hemicelluloses). To overcome this issue, in planta engineering to reduce LC recalcitrance has been ongoing in recent decades, with essential input from synthetic biology owing to the complexity of LC pathways and the massive number of genes involved. In this review, we describe recent advances in LC manipulation and eight strategies for redesigning the pathways for lignin and structural glycans to reduce LC recalcitrance while mitigating against the growth penalty associated with yield loss.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 3","pages":"213-230"},"PeriodicalIF":1.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous production of corosolic acid, a phyto-insulin, in agroinfiltrated Nicotiana benthamiana leaves.

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-09-25 DOI: 10.5511/plantbiotechnology.24.0420a

Jutapat Romsuk, Pisanee Srisawat, Jekson Robertlee, Shuhei Yasumoto, Kenji Miura, Toshiya Muranaka, Hikaru Seki

{"title":"Heterologous production of corosolic acid, a phyto-insulin, in agroinfiltrated Nicotiana benthamiana leaves.","authors":"Jutapat Romsuk, Pisanee Srisawat, Jekson Robertlee, Shuhei Yasumoto, Kenji Miura, Toshiya Muranaka, Hikaru Seki","doi":"10.5511/plantbiotechnology.24.0420a","DOIUrl":"10.5511/plantbiotechnology.24.0420a","url":null,"abstract":"Triterpenoids, a group of specialized plant metabolites with substantial structural diversity, are promising for healthcare applications. Ursolic acid, a pentacyclic triterpenoid with therapeutic potential, is also important as a precursor of corosolic acid, which is known as a \"phyto-insulin\" for its insulin-like properties. Ursolic acid is synthesized from a linear 30-carbon precursor 2,3-oxidosqualene via cyclization to produce triterpene scaffold α-amyrin, followed by a series of oxidation steps at the C-28 position mediated by cytochrome P450 monooxygenases (CYPs) in the CYP716A subfamily. The Tsukuba system was developed for the high-level transient expression of foreign proteins in plant cells based on the use of a binary vector equipped with geminiviral replication system and a double terminator. In this study, we used the Tsukuba system to produce ursolic acid in Nicotiana benthamiana leaves via transient pathway reconstruction. We used an oxidosqualene cyclase identified from the medicinal legume Bauhinia forficata, exhibiting a preponderant α-amyrin-producing activity. Wild-type Medicago truncatula CYP716A12 and its mutants were assessed in terms of ursolic acid production. We improved the performance of MtCYP716A12 by co-expressing it with the appropriate cytochrome P450 reductase (CPR) isozyme as an electron-transfer partner and tested different Agrobacterium infiltration ratios to optimize the CPR : CYP ratio to maximize ursolic acid production. We also achieved high yield of corosolic acid by co-expressing Avicennia marina CYP716C53 with ursolic acid biosynthetic enzymes. Moreover, engineering of AmCYP716C53 significantly improved corosolic acid yield, resulting in a yield exceeding the content found in banaba leaves, a well-known rich source of corosolic acid.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 3","pages":"277-288"},"PeriodicalIF":1.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated metabolite profiling and transcriptome analysis reveal candidate genes involved in the biosynthesis of benzylisoquinoline alkaloids in Corydalis solida.

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-09-25 DOI: 10.5511/plantbiotechnology.24.0205a

Yasuyuki Yamada, Emi Tamagaki, Nobukazu Shitan, Fumihiko Sato

{"title":"Integrated metabolite profiling and transcriptome analysis reveal candidate genes involved in the biosynthesis of benzylisoquinoline alkaloids in Corydalis solida.","authors":"Yasuyuki Yamada, Emi Tamagaki, Nobukazu Shitan, Fumihiko Sato","doi":"10.5511/plantbiotechnology.24.0205a","DOIUrl":"10.5511/plantbiotechnology.24.0205a","url":null,"abstract":"Structurally diverse benzylisoquinoline alkaloids (BIAs) are found in specific plant families, some of which are desirable for their efficient production because of their strong biological activities. Corydalis plants (e.g., Corydalis yanhusuo) of the family Papaveraceae also produce various BIAs; thus, they have been used in traditional Chinese medicine. Because metabolic engineering and synthetic biology using microorganisms are promising technologies for the effective production of useful metabolites, elucidation of the biosynthetic pathway of each BIA is indispensable. Although several enzyme genes involved in the biosynthesis of Corydalis BIAs have recently been isolated, many remain unknown, such as the protoberberine alkaloid C-methyltransferase involved in the biosynthesis of corydaline, one of the main BIAs found in the tubers of Corydalis plants. In this study, we performed transcriptome analysis combined with metabolite profiling of different tissues of Corydalis solida. Based on the high accumulation of several BIAs, including protopine, allocryptopine, and corydaline, genes encoding putative biosynthetic enzymes, including cytochrome P450, methyltransferase, and oxidase proteins, that were highly expressed in the tubers were screened. Two OMT genes, CsOMT1 and CsOMT2, were highly expressed in the tuber, and further characterization using crude enzyme preparations demonstrated that CsOMT1 showed 7-O-methylation activity against reticuline, whereas CsOMT2 catalyzed 9-O-methylation of scoulerine, followed by 2-O-methylation of tetrahydrocolumbamine. Our findings provide valuable information for the isolation of novel biosynthetic enzyme genes in Corydalis species.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 3","pages":"267-276"},"PeriodicalIF":1.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preface to the special issue "Synthetic Biology and Bioproduction in Plants".

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-09-25 DOI: 10.5511/plantbiotechnology.24.0000p

Nobutaka Mitsuda, Hikaru Seki, Tsubasa Shoji, Masami Yokota Hirai

引用次数: 0

Evaluation of host status of garlic varieties for a plant-parasitic nematode, Ditylenchus destructor, by using in vitro inoculation. 利用离体接种法评估大蒜品种对植物寄生线虫毁灭天牛的寄主状况。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0428a

Kazuki Tadamura, Atsushi Torada, Toyoshi Yoshiga

{"title":"Evaluation of host status of garlic varieties for a plant-parasitic nematode, Ditylenchus destructor, by using in vitro inoculation.","authors":"Kazuki Tadamura, Atsushi Torada, Toyoshi Yoshiga","doi":"10.5511/plantbiotechnology.24.0428a","DOIUrl":"10.5511/plantbiotechnology.24.0428a","url":null,"abstract":"Ditylenchus destructor is a plant-parasitic nematode that severely damages garlic (Allium sativum L.) in Japan. D. destructor is detected in roots, bulbs, and outer bulb skins of garlic at harvest; however, the resistance of garlic to D. destructor infection is not well understood. Here, we investigated the propagation of D. destructor in storage organs and roots using in vitro plantlets of six Japanese garlic varieties to exclude the effects of microbes and to uniform growing conditions. In vitro inoculation can proceed simultaneously with vegetative growth, storage organ formation of garlic plantlets, and D. destructor infection. In 'Fukuchi-white', a variety susceptible to D. destructor, nematodes successfully propagated in storage organs and roots. Furthermore, the nematodes invaded and propagated in the newly formed storage organs. By contrast, 'Kirishima', 'Hirado', and 'Shishimaru' substantially suppressed more the propagation of the nematodes in storage organs and roots than 'Fukuchi-white'. Additionally, the propagation of nematodes in newly formed storage organs was inhibited in these three varieties. 'Shishimaru' showed unique responses to D. destructor infection: nematode propagation was the lowest among six varieties in inoculation tests and the nematode-inoculated cloves turned brown. Our results suggest that several garlic varieties have resistance mechanisms that suppress the propagation of D. destructor in storage organs and roots, and that in vitro inoculation methods are useful for selecting resistant garlic varieties. These findings will help developing novel D. destructor-resistant garlic varieties and our further understanding of garlic-nematode interactions.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 2","pages":"137-145"},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Oryza sativa for lignin augmentation and structural simplification. 用于增加木质素和简化结构的黑麦草代谢工程。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0131a

Toshiaki Umezawa

引用次数: 0

Downregulation of a Phi class glutathione S-transferase gene in transgenic torenia yielded pale flower color. 在转基因香椿中下调 Phi 类谷胱甘肽 S 转移酶基因会导致花色变淡。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0409a

Misako Akagi, Noriko Nakamura, Yoshikazu Tanaka

引用次数: 0

Rice KORPOKKUR gene is expressed in mitotic cells and regulates pleiotropic features during vegetative phase. 水稻 KORPOKKUR 基因在有丝分裂细胞中表达，并在无性繁殖期调节多生物特征。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0305a

Kaito Chiba, Takumi Tezuka, Hiroetsu Wabiko, Yasuo Nagato, Nobuhiro Nagasawa, Namiko Satoh-Nagasawa

引用次数: 0

The blue light signaling inhibitor 3-bromo-7-nitroindazole affects gene translation at the initial reception of blue light in young Arabidopsis seedlings. 蓝光信号抑制剂 3-bromo-7-nitroindazole 会影响拟南芥幼苗最初接受蓝光时的基因翻译。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0323a

Yukio Kurihara, Chika Akagi, Yuko Makita, Masaharu Kawauchi, Emiko Okubo-Kurihara, Tomohiko Tsuge, Takashi Aoyama, Minami Matsui

{"title":"The blue light signaling inhibitor 3-bromo-7-nitroindazole affects gene translation at the initial reception of blue light in young Arabidopsis seedlings.","authors":"Yukio Kurihara, Chika Akagi, Yuko Makita, Masaharu Kawauchi, Emiko Okubo-Kurihara, Tomohiko Tsuge, Takashi Aoyama, Minami Matsui","doi":"10.5511/plantbiotechnology.24.0323a","DOIUrl":"10.5511/plantbiotechnology.24.0323a","url":null,"abstract":"Initial light reception after germination is a dramatic life event when a seedling starts proper morphogenesis. Blue light contains a range of light wavelengths that plants can perceive. A previous report suggested that the chemical compound 3-bromo-7-nitroindazole (3B7N) inhibits blue light-mediated suppression of hypocotyl elongation by physically interacting with the blue light receptor Cryptochrome 1 (CRY1). We previously examined changes of genome-wide gene expression in Arabidopsis seedlings germinated in the dark and then exposed to blue light by RNA-seq and Ribo-seq analyses. The expression of ribosome-related genes was translationally upregulated in response to the initial blue light exposure, depending on signals from both the nucleus and chloroplasts. Here, we re-analyzed our previous data and examined the effect of 3B7N treatment on changes in gene expression upon blue light exposure. The results showed that 3B7N negatively affected translation of ribosome-related genes and, interestingly, the effects were similar to not only those in cry1cry2 mutants but also plants under suppression of photosynthesis. We propose an apparent crosstalk between chloroplast function and blue light signaling.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 2","pages":"153-157"},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eugenol transport and biosynthesis through grafting in aromatic plants of the Ocimum genus. 欧芹属芳香植物通过嫁接进行的丁香酚运输和生物合成。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0124a

Shogo Hirose, Kaito Sakai, Sawa Kobayashi, Masato Tsuro, Atsushi Morikami, Hironaka Tsukagoshi

{"title":"Eugenol transport and biosynthesis through grafting in aromatic plants of the Ocimum genus.","authors":"Shogo Hirose, Kaito Sakai, Sawa Kobayashi, Masato Tsuro, Atsushi Morikami, Hironaka Tsukagoshi","doi":"10.5511/plantbiotechnology.24.0124a","DOIUrl":"10.5511/plantbiotechnology.24.0124a","url":null,"abstract":"Aromatic compounds play essential roles in plant physiology and various industries because of their unique fragrances and beneficial properties. In this study, we investigated the transport and biosynthesis of eugenol, a prominent aromatic compound, within the Ocimum genus, using grafting experiments. Grafting sweet basil (Ocimum basilicum) scions onto diverse rootstocks, including tobacco (Nicotiana benthamiana) and thyme (Thymus vulgaris), revealed that eugenol is transported from the shoot to the root across distinct plant species. Furthermore, grafting within the Ocimum genus, which includes O. basilicum, O. tenuiflorum, and O. americanum, resulted in variations in eugenol transport and accumulation. The eugenol content in the shoots remained constant across all combinations, whereas the root eugenol levels varied depending on the scion-rootstock pair. To elucidate the biosynthetic capabilities of eugenol in Ocimum roots, we performed in vitro enzyme assays using crude protein extracts from roots, which revealed that eugenol can be synthesized in roots in addition to being transported. Expression analysis of eugenol synthase (EGSs) genes showed that EGS4 expression was influenced by grafting in O. basilicum roots, suggesting compensation by other EGSs. Our results suggest that eugenol transport and biosynthesis are multifaceted processes influenced by the interactions between different species and tissues. The potential to engineer eugenol levels in rootstocks lacking biosynthetic capacity has potential applications in agriculture and industry. This study reveals the dynamic interplay between eugenol transport and biosynthesis in the Ocimum genus, providing insights into the manipulation of aromatic compound production in plants.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 2","pages":"111-120"},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0