Plant Biotechnology最新文献

{"title":"Transcriptional regulation of cell proliferation competence-associated Arabidopsis genes, <i>CDKA;1</i>, <i>RID1</i> and <i>SRD2</i>, by phytohormones in tissue culture.","authors":"Natsu Takayanagi,&nbsp;Mai Mukai,&nbsp;Munetaka Sugiyama,&nbsp;Misato Ohtani","doi":"10.5511/plantbiotechnology.22.0513a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.0513a","url":null,"abstract":"<p><p>During organ regeneration, differentiated cells acquire cell proliferation competence before the re-start of cell division. In <i>Arabidopsis thaliana</i> (Arabidopsis), CDKA;1, a cyclin-dependent kinase, RID1, a DEAH-box RNA helicase, and SRD2, a small nuclear RNA transcription factor, are implicated in the regulation of cell proliferation competence. Here, we report phytohormonal transcriptional regulation of these cell proliferation competence-associated genes during callus initiation. We can induce the callus initiation from Arabidopsis hypocotyl explants by the culture on the auxin-containing medium. By RT-quantitative PCR analysis, we observed higher mRNA accumulation of <i>CDKA;1</i>, <i>RID1</i>, and <i>SRD2</i> in culture on the auxin-containing medium than in culture on the auxin-free medium. Promoter-reporter analysis showed that the <i>CDKA;1</i>, <i>RID1</i>, and <i>SRD2</i> expression was induced in the stele regions containing pericycle cells, where cell division would be resumed to make callus, by the culture in the medium containing auxin and/or cytokinin. However, the expression levels of these genes in cortical and epidermal cells, which would not originate callus cells, were variable by genes and phytohormonal conditions. We also found that the <i>rid1-1</i> mutation greatly decreased the expression levels of <i>CDKA;1</i> and <i>SRD2</i> during callus initiation specifically at 28°C (restrictive temperature), while the <i>srd2-1</i> mutation did not obviously decrease the expression levels of <i>CDKA;1</i> and <i>RID1</i> regardless of temperature conditions but rather even increased them at 22°C (permissive temperature). Together, our results implicated the phytohormonal and differential regulation of cell proliferation competence-associated genes in the multistep regulation of cell proliferation competence.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592934/pdf/plantbiotechnology-39-3-22.0513a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40688641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of benzylglucosinolate in genetically engineered carrot suspension cultures.","authors":"Elena Kurzbach,&nbsp;Matthias Strieker,&nbsp;Ute Wittstock","doi":"10.5511/plantbiotechnology.22.0509a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.0509a","url":null,"abstract":"<p><p>Glucosinolates, a group of sulfur-containing specialized metabolites of the Brassicales, have attracted a lot of interest in nutrition, medicine and agriculture due to their positive health effects and their involvement in plant defense. Their biological activities and the extensive knowledge of their biosynthesis have inspired research into development of crops with enhanced glucosinolate contents as well as their biotechnological production in homologous and heterologous systems. Here, we provide proof-of-concept for transgenic suspension cultures of carrot (<i>Daucus carota</i>, Apiacae) as a scalable production platform for plant specialized metabolites using benzylglucosinolate as a model. Two T-DNAs carrying in total six genes of the benzylglucosinolate biosynthesis pathway from <i>Arabidopsis thaliana</i> as well as <i>NPTII</i> and <i>BAR</i> as selectable markers were transferred to carrot cells by <i>Agrobacterium tumefaciens</i>-mediated transformation. Putative transformants selected based on their kanamycin and BASTA resistances were subjected to HPLC-MS analysis. Of 79 putative transformants, 17 produced benzylglucosinolate. T-DNA-integration was confirmed for the five best producers. Callus from these transformants was used to establish suspension cultures for quantitative analysis. When grown in 60-ml-cultures, the best transformants produced roughly 2.5 nmol (g fw)^-1 benzylglucosinolate, together with up to 10 nmol (g fw)^-1 desulfobenzylglucosinolate. Only one transformant produced more benzylglucosinolate than desulfobenzylglucosinolate. The concentration of sulfate in the medium was not a major limiting factor. High production seemed to be associated with poor growth and vice versa. Therefore, future research should try to optimize medium and cultivation process and to separate growth and production phase by using an inducible promoter.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592945/pdf/plantbiotechnology-39-3-22.0509a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40462090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IRE1-mediated cytoplasmic splicing and regulated IRE1-dependent decay of mRNA in the liverwort <i>Marchantia polymorpha</i>.","authors":"Sho Takeda,&nbsp;Taisuke Togawa,&nbsp;Kei-Ichiro Mishiba,&nbsp;Katsuyuki T Yamato,&nbsp;Yuji Iwata,&nbsp;Nozomu Koizumi","doi":"10.5511/plantbiotechnology.22.0704a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.0704a","url":null,"abstract":"<p><p>The unfolded protein response (UPR) or the endoplasmic reticulum (ER) stress response is a homeostatic cellular response conserved in eukaryotes to alleviate the accumulation of unfolded proteins in the ER. In the present study, we characterized the UPR in the liverwort <i>Marchantia polymorpha</i> to obtain insights into the conservation and divergence of the UPR in the land plants. We demonstrate that the most conserved UPR transducer in eukaryotes, IRE1, is conserved in <i>M. polymorpha</i>, which harbors a single gene encoding IRE1. We showed that MpIRE1 mediates cytoplasmic splicing of mRNA encoding MpbZIP7, a <i>M. polymorpha</i> homolog of bZIP60 in flowering plants, and upregulation of ER chaperone genes in response to the ER stress inducer tunicamycin. We further showed that MpIRE1 also mediates downregulation of genes encoding secretory and membrane proteins in response to ER stress, indicating the conservation of regulated IRE1-dependent decay of mRNA. Consistent with their roles in the UPR, Mp<i>ire1</i> ^<i>ge</i> and Mp<i>bzip7</i> ^<i>ge</i> mutants exhibited higher sensitivity to ER stress. Furthermore, an Mp<i>ire1</i> ^<i>ge</i> mutant also exhibited retarded growth even without ER stress inducers, indicating the importance of MpIRE1 for vegetative growth in addition to alleviation of ER stress. The present study provides insights into the evolution of the UPR in land plants.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592932/pdf/plantbiotechnology-39-3-22.0704a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40688642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetraploidization promotes radial stem growth in poplars.","authors":"Chikage Umeda-Hara,&nbsp;Hidekazu Iwakawa,&nbsp;Misato Ohtani,&nbsp;Taku Demura,&nbsp;Tomoko Matsumoto,&nbsp;Jun Kikuchi,&nbsp;Koji Murata,&nbsp;Masaaki Umeda","doi":"10.5511/plantbiotechnology.22.0716a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.0716a","url":null,"abstract":"<p><p>Somatic polyploidization often increases cell and organ size, thereby contributing to plant biomass production. However, as most woody plants do not undergo polyploidization, explaining the polyploidization effect on organ growth in trees remains difficult. Here we developed a new method to generate tetraploid lines in poplars through colchicine treatment of lateral buds. We found that tetraploidization induced cell enlargement in the stem, suggesting that polyploidization can increase cell size in woody plants that cannot induce polyploidization in normal development. Greenhouse growth analysis revealed that radial growth was enhanced in the basal stem of tetraploids, whereas longitudinal growth was retarded, producing the same amount of stem biomass as diploids. Woody biomass characteristics were also comparable in terms of wood substance density, saccharification efficiency, and cell wall profiling. Our results reveal tetraploidization as an effective strategy for improving woody biomass production when combined with technologies that promote longitudinal stem growth by enhancing metabolite production and/or transport.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592956/pdf/plantbiotechnology-39-3-22.0716a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40688643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of GSL8 in low calcium tolerance in <i>Arabidopsis thaliana</i>.","authors":"Yusuke Shikanai,&nbsp;Mayu Asada,&nbsp;Takafumi Sato,&nbsp;Yusuke Enomoto,&nbsp;Mutsumi Yamagami,&nbsp;Katsushi Yamaguchi,&nbsp;Shuji Shigenobu,&nbsp;Takehiro Kamiya,&nbsp;Toru Fujiwara","doi":"10.5511/plantbiotechnology.22.0421a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.0421a","url":null,"abstract":"<p><p>Calcium (Ca) deficiency affects the yields and quality of agricultural products. Susceptibility to Ca deficiency varies among crops and cultivars; however, its genetic basis remains largely unknown. Genes required for low Ca tolerance in <i>Arabidopsis thaliana</i> have been identified. In this study, we identified a novel gene required for low Ca tolerance in <i>A. thaliana</i>. We isolated a mutant sensitive to low Ca concentrations and identified <i>Glucan synthase-like</i> (<i>GSL</i>) <i>8</i> as a gene responsible for low Ca tolerance. <i>GSL8</i> is a paralog of the previously identified low Ca tolerance gene <i>GSL10</i>, which encodes β-1,3 glucan(callose) synthase. Under low Ca conditions, the shoot growth of <i>gsl8</i> mutants were inhibited compared to wild-type plants. A grafting experiment indicated that the shoot, but not root, genotype was important for the shoot growth phenotype. The ectopic accumulation of callose under low Ca conditions was reduced in <i>gsl8</i> mutants. We further investigated the interaction between <i>GSL8</i> and <i>GSL10</i> by testing the <i>gsl8 gsl10</i> double mutant for sensitivity to low Ca concentrations. The double mutant exhibited a more severe phenotype than the single mutant under 0.3 mM Ca, indicating additive effects of <i>GSL8</i> and <i>GSL10</i> with respect to low Ca tolerance. These results establish that <i>GSL</i> genes are required for low Ca tolerance in <i>A. thaliana</i>.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592936/pdf/plantbiotechnology-39-3-22.0421a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40462087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dear prospective authors of <i>Plant Biotechnology</i>.","authors":"","doi":"10.5511/plantbiotechnology.22.editorial3","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.editorial3","url":null,"abstract":"","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592928/pdf/plantbiotechnology-39-3-22.editional3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40673508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early flowering phenotype of the Arabidopsis <i>altered meristem program1</i> mutant is dependent on the FLOWERING LOCUS T-mediated pathway.","authors":"Takashi Nobusawa,&nbsp;Hiroshi Yamatani,&nbsp;Makoto Kusaba","doi":"10.5511/plantbiotechnology.22.0515a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.0515a","url":null,"abstract":"<p><p>Controlling the flowering time is crucial for propagating plant species and crop production. <i>ALTERED MERISTEM PROGRAM1</i> (<i>AMP1</i>) in <i>Arabidopsis thaliana</i> encodes a putative carboxypeptidase, and an <i>AMP1</i> mutant (<i>amp1</i>) was found to cause highly pleiotropic phenotypes including a short plastochron, an enlarged shoot apical meristem, and reduced apical dominance. Although <i>amp1</i> also shows an early flowering phenotype, its mechanism has not been investigated in detail. The most important floral integrator or florigen gene, <i>FLOWERING LOCUS T</i> (<i>FT</i>), has a close relative, <i>TWIN SISTER OF FT</i> (<i>TSF</i>). In this report, we generated a new allele of <i>tsf</i> using a genome-editing technique and produced <i>ft tsf</i> double and <i>amp1 ft tsf</i> triple mutants. The flowering time of <i>amp1 ft tsf</i> was equally as late as <i>ft tsf</i> under long-day conditions. In addition, the expression level of <i>FT</i> in <i>amp1</i> was 2.4-fold higher than that in wild-type, even five days after germination under long-day conditions. These results suggest that the elevated expression level of <i>FT</i> is responsible for the early flowering phenotype of <i>amp1</i>. Furthermore, expression of <i>FLOWERING LOCUS C</i> (<i>FLC</i>), a negative regulator of <i>FT</i> expression, is severely repressed in <i>amp1</i>, raising the possibility that low expression levels of <i>FLC</i> contributes to upregulation of <i>FT</i> expression and the early flowering phenotype of <i>amp1</i>.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592955/pdf/plantbiotechnology-39-3-22.0515a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40688639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive effects of heavy-ion beam irradiation on sweet potato (<i>Ipomoea batatas</i> [L.] Lam.).","authors":"Hyungjun Park, Yosuke Narasako, Tomoko Abe, Hisato Kunitake, Tomonari Hirano","doi":"10.5511/plantbiotechnology.22.0725a","DOIUrl":"10.5511/plantbiotechnology.22.0725a","url":null,"abstract":"<p><p>Sweet potato is a major root crop with nutritious tuberous roots. The mechanism of tuberous root development has not yet been adequately elucidated. Genetic resources are required to develop the molecular understanding of sweet potato. Heavy-ion beams were applied to hexaploid sweet potato for an increase in genetic variation, after which the comprehensive effects of heavy-ion beam irradiation were investigated. In vitro cultured shoots with an axillary bud of 'Beniharuka' were irradiated with Ar-ions at a dose of 1-5 Gy and C-ions at a dose of 5-20 Gy, and three irradiated lines were separated from each irradiated shoot. The shoot regeneration was inhibited at high doses of each ion irradiation. Ar-ion irradiation had an especially high biological effect on shoot regeneration. A total of 335 lines were obtained, consisting of 104 and 231 lines derived from Ar- and C-ion irradiation, respectively. The change in the DNA content of the lines was analyzed by flow cytometry to evaluate the irradiation-induced damage to the DNA. The two lines demonstrated significant differences in the DNA content and changes at the chromosome level. The screening for the morphological mutants was conducted in the field. Some irradiated lines showed inhibited or no tuberous root phenotype as mutant candidates. Additionally, the high-yield mutant candidates were dominated by Ar-ion irradiation. It was indicated that heavy-ion beam mutagenesis is effective in broadening the range of the phenotypes corresponding to tuberous root formation in hexaploid sweet potato.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592942/pdf/plantbiotechnology-39-3-22.0725a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40688637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxicam-type nonsteroidal anti-inflammatory drugs enhance <i>Agrobacterium</i>-mediated transient transformation in plants.","authors":"Seung-Won Choi,&nbsp;Kie Kumaishi,&nbsp;Reiko Motohashi,&nbsp;Harumi Enoki,&nbsp;Wiluk Chacuttayapong,&nbsp;Tadashi Takamizo,&nbsp;Hiroaki Saika,&nbsp;Masaki Endo,&nbsp;Tetsuya Yamada,&nbsp;Aya Hirose,&nbsp;Nobuya Koizuka,&nbsp;Seisuke Kimura,&nbsp;Yaichi Kawakatsu,&nbsp;Hiroyuki Koga,&nbsp;Emi Ito,&nbsp;Ken Shirasu,&nbsp;Yasunori Ichihashi","doi":"10.5511/plantbiotechnology.22.0312a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.0312a","url":null,"abstract":"<p><p><i>Agrobacterium</i>-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique. In this study, we discovered that oxicam-type nonsteroidal anti-inflammatory drugs, including tenoxicam (TNX), increase the efficiency of <i>Agrobacterium</i>-mediated transient transformation. TNX treatment increased the transformation efficiency of <i>Agrobacterium</i>-mediated transformation of <i>Arabidopsis thaliana</i> mature leaves by agroinfiltration. The increase of efficiency by TNX treatment was not observed in <i>dde2/ein2/pad4/sid2</i> quadruple mutant, indicating that TNX inhibits the immune system mediated by jasmonic acid, ethylene, and salicylic acid against to <i>Agrobacterium</i>. We also found that TNX-treatment is applicable for the transient expression and subcellular localization analysis of fluorescent-tagged proteins in Arabidopsis leaf cells. In addition, we found that TNX increases the efficiency of <i>Agrobacterium</i>-mediated transient transformation of Jatropha. Given that treatment with oxicam compounds is a simple and cost effective method, our findings will provide a new option to overcome limitations associated with <i>Agrobacterium</i>-mediated transformation of various plant species.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592935/pdf/plantbiotechnology-39-3-22.0312a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40462089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in <i>Nicotiana benthamiana</i> leaves.","authors":"Edjohn Aaron Macauyag,&nbsp;Hiroyuki Kajiura,&nbsp;Takao Ohashi,&nbsp;Ryo Misaki,&nbsp;Kazuhito Fujiyama","doi":"10.5511/plantbiotechnology.22.0628a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.0628a","url":null,"abstract":"<p><p>The human basic fibroblast growth factor (bFGF) is a protein that plays a pivotal role in cellular processes like cell proliferation and development. As a result, it has become an important component in cell culture systems, with applications in biomedical engineering, cosmetics, and research. Alternative production techniques, such as transient production in plants, are becoming a feasible option as the demand continues to grow. High-level bFGF production was achieved in this study employing an optimized <i>Agrobacterium</i>-mediated transient expression system, which yielded about a 3-fold increase in production over a conventional system. This yield was further doubled at about 185 µg g^-1 FW using a mutant protease-resistant version that degraded/aggregated at a three-fold slower rate in leaf crude extracts. To achieve a pure product, a two-step purification technique was applied. The capacity of the pure protease-resistant bFGF (PRbFGF) to stimulate cell proliferation was tested and was found to be comparable to that of <i>E. coli</i>-produced bFGF in HepG2 and CHO-K1 cells. Overall, this study demonstrates a high-level transient production system of functional PRbFGF in <i>N. benthamiana</i> leaves as well as an efficient tag-less purification technique of leaf crude extracts.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592933/pdf/plantbiotechnology-39-3-22.0628a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40688638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}