Plant Biotechnology最新文献_第10页

Effect of the CLE14 polypeptide on GLABRA2 homolog gene expression in rice and tomato roots. CLE14多肽对水稻和番茄根GLABRA2同源基因表达的影响。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0725a

N. Hayashi, Natthanon Rongkavilit, T. Tetsumura, S. Sawa, T. Wada, R. Tominaga-Wada

{"title":"Effect of the CLE14 polypeptide on GLABRA2 homolog gene expression in rice and tomato roots.","authors":"N. Hayashi, Natthanon Rongkavilit, T. Tetsumura, S. Sawa, T. Wada, R. Tominaga-Wada","doi":"10.5511/plantbiotechnology.19.0725a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0725a","url":null,"abstract":"The CLAVATA3/ESR (CLE) plant polypeptides act as peptide hormones in various physiological and developmental aspects in a diverse array of land plants. One of the CLE family of genes, CLE14, is reported to induce root hair formation in Arabidopsis thaliana roots. Previously, we demonstrated that the application of synthetic CLE14 polypeptide treatment induced excess root hairs, and reduced the expression level of the non-hair cell fate determinant gene, GLABRA2 (GL2) in Arabidopsis roots. In this study, we investigated the function of synthetic CLE14 polypeptide in rice (Oryza sativa) and tomato (Solanum lycopersicum) roots. We measured the expression levels of the OsGL2 and SlGL2 genes, i.e., homologs of the Arabidopsis GL2 gene, in rice and tomato seedlings, respectively. Although CLE14 polypeptide treatment induced excess root hair formation in rice roots, substantial root hair induction was not observed in tomato roots. However, the CLE14 polypeptide treatment significantly inhibited the expression of the GL2 homolog genes of rice (OsGL2) and tomato (SlGL2). Our findings thus indicated that CLE14 can inhibit the GL2 gene expression in both rice and tomato plants, similar to the effect seen in Arabidopsis.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"205-208"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42056984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Copper treatment of peach leaves causes lesion formation similar to the biotic stress response. 铜处理桃叶会导致类似于生物应激反应的损伤形成。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0531b

F. Goto, Yusuke Enomoto, K. Shoji, H. Shimada, T. Yoshihara

{"title":"Copper treatment of peach leaves causes lesion formation similar to the biotic stress response.","authors":"F. Goto, Yusuke Enomoto, K. Shoji, H. Shimada, T. Yoshihara","doi":"10.5511/plantbiotechnology.19.0531b","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0531b","url":null,"abstract":"Peach (Prunus persica (L.) Batsch) leaves are sensitive to copper (Cu) exposure. The symptoms of Cu exposure are similar to those of bacterial spot disease; however, the mechanism underlying lesion formation caused by Cu exposure is not clear. Here, we investigated whether lesion formation caused by Cu exposure was related to the mechanism underlying plant resistance to microbial pathogens. When Cu was applied to the centre of a pinhole on peach leaves, a two-step process was observed. A pale green section in the shape of a doughnut, located far from a Cu treatment point, first appeared on a leaf treated with 2 mM CuSO4. Next, a yellow-white section gradually spread from the Cu treatment point to the pale green section. Finally, a gap was formed in the middle of the pale green section. The inner part of the pale green section contained 96% of the Cu applied, indicating that Cu is retained in the lesion area. Real-time PCR analysis of the expression of genes encoding pathogenesis-related proteins and enzymes involved in phytoalexin synthesis revealed that three genes (encoding chitinase, pathogenesis-related protein 4, and β-1,3-glucanase-3) of the eight tested were upregulated by Cu treatment. Furthermore, treatment with caspase-1 inhibitors reduced lesion formation. These results show that Cu treatment of peach leaves causes cell death similar to that occurring during the biotic stress response.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"135-142"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0531b","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48887854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparative co-expression network analysis extracts the SlHSP70 gene affecting to shoot elongation of tomato. 比较共表达网络分析提取了影响番茄芽伸长的SlHSP70基因。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0603a

Nam Tuan Vu, Ken Kamiya, A. Fukushima, Shuhei Hao, W. Ning, T. Ariizumi, H. Ezura, M. Kusano

{"title":"Comparative co-expression network analysis extracts the SlHSP70 gene affecting to shoot elongation of tomato.","authors":"Nam Tuan Vu, Ken Kamiya, A. Fukushima, Shuhei Hao, W. Ning, T. Ariizumi, H. Ezura, M. Kusano","doi":"10.5511/plantbiotechnology.19.0603a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0603a","url":null,"abstract":"Tomato is one of vegetables crops that has the highest value in the world. Thus, researchers are continually improving the agronomical traits of tomato fruits. Auxins and gibberellins regulate plant growth and development. Aux/indole-3-acetic acid 9 (SlIAA9) and the gene encoding the DELLA protein (SlDELLA) are well-known genes that regulate plant growth and development, including fruit set and enlargement by cell division and cell expansion. The absence of tomato SlIAA9 and SlDELLA results in abnormal shoot growth and leaf shape and giving rise to parthenocarpy. To investigate the key regulators that exist up- or downstream of SlIAA9 and SlDELLA signaling pathways for tomato growth and development, we performed gene co-expression network analysis by using publicly available microarray data to extract genes that are directly connected to the SlIAA9 and SlDELLA nodes, respectively. Consequently, we chose a gene in the group of heat-shock protein (HSP)70s that was connected with the SlIAA9 node and SlDELLA node in each co-expression network. To validate the extent of effect of SlHSP70-1 on tomato growth and development, overexpressing lines of the target gene were generated. We found that overexpression of the targeted SlHSP70-1 resulted in internode elongation, but the overexpressing lines did not show abnormal leaf shape, fruit set, or fruit size when compared with that of the wild type. Our study suggests that the targeted SlHSP70-1 is likely to function in shoot growth, like SlIAA9 and SlDELLA, but it does not contribute to parthenocarpy as well as fruit set. Our study also shows that only a single SlHSP70 out of 25 homologous genes could change the shoot length.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"143-153"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0603a","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43053378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Association analysis of phenotypic and metabolomic changes in Arabidopsis accessions and their F1 hybrids affected by different photoperiod and sucrose supply. 不同光周期和蔗糖供应对拟南芥及其F1杂交种表型和代谢组学变化的影响

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0604a

Quynh Thi Ngoc Le, Naoya Sugi, J. Furukawa, Makoto Kobayashi, K. Saito, M. Kusano, H. Shiba

{"title":"Association analysis of phenotypic and metabolomic changes in Arabidopsis accessions and their F1 hybrids affected by different photoperiod and sucrose supply.","authors":"Quynh Thi Ngoc Le, Naoya Sugi, J. Furukawa, Makoto Kobayashi, K. Saito, M. Kusano, H. Shiba","doi":"10.5511/plantbiotechnology.19.0604a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0604a","url":null,"abstract":"Photoperiod and sucrose (Suc) assimilation play important roles in the regulation of plant growth and development. However, it remains unclear how natural variation of plants could contribute to metabolic changes under various growth conditions. Here, we investigated the developmental and metabolomic responses of two natural accessions of Arabidopsis thaliana, Columbia (Col) and C24, and their reciprocal F1 hybrids grown under four carbon source regimens, i.e., two different photoperiods and the presence or absence of exogenous Suc supply. The effect of exogenous Suc clearly appeared in the growth of Col and the F1 hybrid but not in C24, whereas long-day conditions had significant positive effects on the growth of all lines. Comparative metabolite profiling of Col, C24, and the F1 hybrid revealed that changes in metabolite levels, particularly sugars, were highly dependent on genotype-specific responses rather than growth conditions. The presence of Suc led to over-accumulation of seven metabolites, including four sugars, a polyamine, and two amino acids in C24, whereas no such accumulation was observed in the profiles of Col and the F1 hybrid. Thus, the comparative metabolite profiling revealed that the two parental lines of the hybrid show a distinct difference in sugar metabolism.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"155-165"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0604a","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43190613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Production and characterization of intersectional hybrids between Tricyrtis sect. Brachycyrtis and sect. Hirtae via ovule culture. 经胚珠培养的毛菖蒲节短菖蒲与毛菖蒲节交系杂交种的产生及性状研究。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0807a

Toshiya Inamura, Manami Nakazawa, Mitsuyo Ishibe, M. Otani, M. Nakano

{"title":"Production and characterization of intersectional hybrids between Tricyrtis sect. Brachycyrtis and sect. Hirtae via ovule culture.","authors":"Toshiya Inamura, Manami Nakazawa, Mitsuyo Ishibe, M. Otani, M. Nakano","doi":"10.5511/plantbiotechnology.19.0807a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0807a","url":null,"abstract":"The liliaceous perennial plants, Tricyrtis spp., have recently become popular as ornamental plants for pot and garden uses. In order to broaden the variability in plant form, flower form and flower color of Tricyrtis spp., intersectional hybridization was examined between four T. formosana cultivars or T. hirta var. albescens (sect. Hirtae) and T. macranthopsis (sect. Brachycyrtis). After cross-pollination, ovary enlargement was observed only when T. macranthopsis was used as a pollen parent. Ovules with placental tissues were excised from enlarged ovaries and cultured on half-strength MS medium without plant growth regulators. From five cross-combinations, 31 ovule culture-derived plantlets were obtained and 20 of them were confirmed to be intersectional hybrids by flow cytometry and inter-simple sequence repeat analyses. Almost all hybrids grew well and produced flowers 1-2 years after transplantation to the greenhouse. Hybrids had semi-cascade-type shoots, which was intermediate between T. formosana cultivars and T. hirta var. albescens (erect-type shoots) and T. macranthopsis (cascade-type shoots). They produced flowers with novel forms and colors compared with the corresponding parents, and some were horticulturally attractive. The results obtained in the present study indicate the validity of intersectional hybridization via ovule culture for breeding of Tricyrtis spp.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"175-180"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0807a","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46730816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization and fine mapping of nonstop glumes 2 (nsg2) mutant in rice (Oryza sativa L.). 水稻不间断颖花2 (nsg2)突变体的鉴定与精细定位。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0506a

Yunfeng Li, Xiao-Qin Zeng, Hui Zhuang, Huan Chen, Ting Zhang, Jun Zhang, Hao Zheng, Jun Tang, Hong-Lei Wang, Suxian Ren, Y. Ling, G. He

{"title":"Characterization and fine mapping of nonstop glumes 2 (nsg2) mutant in rice (Oryza sativa L.).","authors":"Yunfeng Li, Xiao-Qin Zeng, Hui Zhuang, Huan Chen, Ting Zhang, Jun Zhang, Hao Zheng, Jun Tang, Hong-Lei Wang, Suxian Ren, Y. Ling, G. He","doi":"10.5511/plantbiotechnology.19.0506a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0506a","url":null,"abstract":"In cereal crops, the grain number per panicle and the grain yield are greatly affected by the number of florets in a spikelet. In wild-type rice, a spikelet only produces one fertile floret and beneath the floret are a pair of sterile lemmas and a pair of rudimentary glumes. This study characterized a rice spikelet mutant nonstop glumes 2 (nsg2). In the nsg2 mutant, both the sterile lemmas and rudimentary glumes were elongated, and part of sterile lemma looked like a lemma in appearance, shape and size. Detailed histological analysis and qPCR analysis revealed that the sterile lemmas in the nsg2 mutant had homeotically transformed into lemma-like organs. This phenotype indicates that NSG2 is involved in the regulation of spikelet development and supports the long-held view that sterile lemmas were derived from the lemmas of the two lateral florets. This implies that the rice spikelet has the potential to be restored to the \"three florets spikelet\", which may have existed in its ancestors. Genetic analysis reveals that the nsg2 trait is controlled by a single recessive gene. The NSG2 gene was finally mapped between markers R-20 and R-39 on chromosome 7 with a physical region of 180 kb. The two MYB family factors LOC_Os07g44030 and LOC_Os07g44090 might be involved in the development of the spikelet and floral organ, and they were considered as candidate genes of NSG2. These results provide a foundation for map-based cloning and function analysis of NSG2, as well as evidence to support \"three-florets spikelet\" breeding in rice.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"125-134"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0506a","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49060690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Monitoring single-cell bioluminescence of Arabidopsis leaves to quantitatively evaluate the efficiency of a transiently introduced CRISPR/Cas9 system targeting the circadian clock gene ELF3. 监测拟南芥叶片的单细胞生物发光，定量评估瞬时引入的靶向生物钟基因ELF3的CRISPR/Cas9系统的效率。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0531a

Yuki Kanesaka, Masaaki Okada, S. Ito, T. Oyama

{"title":"Monitoring single-cell bioluminescence of Arabidopsis leaves to quantitatively evaluate the efficiency of a transiently introduced CRISPR/Cas9 system targeting the circadian clock gene ELF3.","authors":"Yuki Kanesaka, Masaaki Okada, S. Ito, T. Oyama","doi":"10.5511/plantbiotechnology.19.0531a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0531a","url":null,"abstract":"The rapid assessment of gene function is crucial in biological research. The CRISPR/Cas9 system is widely used as a tool for targeted gene editing in many organisms including plants. Previously, we established a transient gene expression system for investigating cellular circadian rhythms in duckweed. In this system, circadian reporters and clock gene effectors-such as overexpressors, RNA interference (RNAi), and CRISPR/Cas9-were introduced into duckweed cells using a particle bombardment method. In the present study, we applied the CRISPR/Cas9 system at a single cell level to Arabidopsis thaliana, a model organism in plant biology. To evaluate the mutation induction efficiency of the system, we monitored single-cell bioluminescence after application of the CRISPR/Cas9 system targeting the ELF3 gene, which is essential for robust circadian rhythmicity. We evaluated the mutation induction efficiency by determining the proportion of cells with impaired circadian rhythms. Three single guide RNAs (sgRNAs) were designed, and the proportion of arrhythmic cells following their use ranged from 32 to 91%. A comparison of the mutation induction efficiencies of diploid and tetraploid Arabidopsis suggested that endoreduplication had a slight effect on efficiency. Taken together, our results demonstrate that the transiently introduced CRISPR/Cas9 system is useful for rapidly assessing the physiological function of target genes in Arabidopsis cells.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"104 5","pages":"187-193"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0531a","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41297367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Measuring plant colors. 测量植物的颜色。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-06-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.19.0322A

Ichiro Kasajima

{"title":"Measuring plant colors.","authors":"Ichiro Kasajima","doi":"10.5511/PLANTBIOTECHNOLOGY.19.0322A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.19.0322A","url":null,"abstract":"Plant colors such as 'green leaf' and 'red apple' are often described based on human sense, even in scientific papers. On the other hand, colors are measured based on colorimetric principles in some papers, especially in the studies of horticultural plants. The science of color measurements ('colorimetry') is not included in any of the popular lectures in schools and universities, thus the principles of color measurements would not be understood by most researchers. The present review will overview the principles of colorimetry, and will introduce colorimetric methods which can be used for scientific measurement of plant colors. That is to say, the reflection spectrum of visible light (380-780 nm) is measured at 5-nm intervals on the surface of leaves or petals in 'Spectrometric Color Measurement' (SCM). The spectral data is multiplied with RGB or XYZ color matching functions and integrated to obtain RGB or XYZ intensities. Alternatively, approximate RGB values are directly obtained in 'Photographic Color Measurement' (PCM). RGB/XYZ intensities are further calculated to obtain 'hue', 'saturation', and 'lightness', the three factors of colors. Colorimetric insights into genetic regulations (such as MYB gene) and physiological regulations (such as alexandrite effect) of plant colors are also described.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 2 1","pages":"63-75"},"PeriodicalIF":1.6,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.19.0322A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43576237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis. 膜定位泛素连接酶ATL8参与拟南芥糖饥饿反应。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-06-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.19.0328A

Yongming Luo, Shoki Aoyama, Y. Fukao, Y. Chiba, Takeo Sato, J. Yamaguchi

{"title":"Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis.","authors":"Yongming Luo, Shoki Aoyama, Y. Fukao, Y. Chiba, Takeo Sato, J. Yamaguchi","doi":"10.5511/PLANTBIOTECHNOLOGY.19.0328A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.19.0328A","url":null,"abstract":"As major components of the ubiquitin system, ubiquitin ligases mediate the transfer of ubiquitin to specific target substrates, thereby playing important roles in regulating a wide range of cellular processes. The Arabidopsis Tóxicos en Levadura (ATL) family is a group of plant-specific RING-type ubiquitin ligases with N-terminal transmembrane-like domains. To date, 91 ATL isoforms have been identified in the Arabidopsis genome, with some reported to regulate plant responses to environmental stresses. However, the functions of most ATLs remain unclear. This study showed that ATL8 is a sugar starvation response gene and that ATL8 expression was significantly increased by sugar starvation conditions but repressed by exogenous sugar supply. The ATL8 protein was found to possess ubiquitin ligase activity in vitro and to localize to membrane-bound compartments in plant cells. In addition, Starch Synthase 4 was identified as a putative interactor with ATL8, suggesting that ATL8 may be involved in modulating starch accumulation in response to sugar availability. These findings suggest that ATL8 functions as a membrane-localized ubiquitin ligase likely to be involved in the adaptation of Arabidopsis plants to sugar starvation stress.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 2 1","pages":"107-112"},"PeriodicalIF":1.6,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.19.0328A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47498134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Agroinfiltration-based efficient transient protein expression in leguminous plants. 豆科植物中基于农业浸润的瞬时蛋白高效表达。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-06-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.19.0220B

Takuya Suzaki, Mai Tsuda, H. Ezura, B. Day, K. Miura

{"title":"Agroinfiltration-based efficient transient protein expression in leguminous plants.","authors":"Takuya Suzaki, Mai Tsuda, H. Ezura, B. Day, K. Miura","doi":"10.5511/PLANTBIOTECHNOLOGY.19.0220B","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.19.0220B","url":null,"abstract":"Transient protein expression is an effective tool to rapidly unravel novel gene functions, such as transcriptional activity of promoters and sub-cellular localization of proteins. However, transient expression is not applicable to some species and varieties because of insufficient expression levels. We recently developed one of the strongest agroinfiltration-based transient protein expression systems for plant cells, termed 'Tsukuba system.' About 4 mg/g fresh weight of protein expression in Nicotiana benthamiana was obtained using this system. The vector pBYR2HS, which contains a geminiviral replication system and a double terminator, can be used in various plant species and varieties, including lettuces, eggplants, tomatoes, hot peppers, and orchids. In this study, we assessed the applicability of the Tsukuba system to several species of legumes, including Lotus japonicus, soybean Glycine max, and common bean Phaseolus vulgaris. The GFP protein was transiently expressed in the seedpods of all examined legume species, however, protein expression in leaves was observed only in P. vulgaris. Taken together, our system is an effective tool to examine gene function rapidly in several legume species based on transient protein expression.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 2 1","pages":"119-123"},"PeriodicalIF":1.6,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.19.0220B","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44198260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17