Plant Biotechnology最新文献

Production of cinnamates and benzoates glucose esters by bioconversion using Escherichia coli expressing a glucosyltransferase characterized from sweet potato 利用表达甘薯葡萄糖基转移酶的大肠杆菌进行生物转化，生产肉桂酸盐和苯甲酸盐葡萄糖酯

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-08-09 DOI: 10.5511/plantbiotechnology.24.0705a

Yuki Kobayashi, Nasanjargal Dorjjugder, G. Taguchi

引用次数: 0

Evaluation of host status of garlic varieties for a plant-parasitic nematode, Ditylenchus destructor, by using in vitro inoculation. 利用离体接种法评估大蒜品种对植物寄生线虫毁灭天牛的寄主状况。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0428a

Kazuki Tadamura, Atsushi Torada, Toyoshi Yoshiga

{"title":"Evaluation of host status of garlic varieties for a plant-parasitic nematode, Ditylenchus destructor, by using in vitro inoculation.","authors":"Kazuki Tadamura, Atsushi Torada, Toyoshi Yoshiga","doi":"10.5511/plantbiotechnology.24.0428a","DOIUrl":"10.5511/plantbiotechnology.24.0428a","url":null,"abstract":"Ditylenchus destructor is a plant-parasitic nematode that severely damages garlic (Allium sativum L.) in Japan. D. destructor is detected in roots, bulbs, and outer bulb skins of garlic at harvest; however, the resistance of garlic to D. destructor infection is not well understood. Here, we investigated the propagation of D. destructor in storage organs and roots using in vitro plantlets of six Japanese garlic varieties to exclude the effects of microbes and to uniform growing conditions. In vitro inoculation can proceed simultaneously with vegetative growth, storage organ formation of garlic plantlets, and D. destructor infection. In 'Fukuchi-white', a variety susceptible to D. destructor, nematodes successfully propagated in storage organs and roots. Furthermore, the nematodes invaded and propagated in the newly formed storage organs. By contrast, 'Kirishima', 'Hirado', and 'Shishimaru' substantially suppressed more the propagation of the nematodes in storage organs and roots than 'Fukuchi-white'. Additionally, the propagation of nematodes in newly formed storage organs was inhibited in these three varieties. 'Shishimaru' showed unique responses to D. destructor infection: nematode propagation was the lowest among six varieties in inoculation tests and the nematode-inoculated cloves turned brown. Our results suggest that several garlic varieties have resistance mechanisms that suppress the propagation of D. destructor in storage organs and roots, and that in vitro inoculation methods are useful for selecting resistant garlic varieties. These findings will help developing novel D. destructor-resistant garlic varieties and our further understanding of garlic-nematode interactions.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Oryza sativa for lignin augmentation and structural simplification. 用于增加木质素和简化结构的黑麦草代谢工程。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0131a

Toshiaki Umezawa

引用次数: 0

Downregulation of a Phi class glutathione S-transferase gene in transgenic torenia yielded pale flower color. 在转基因香椿中下调 Phi 类谷胱甘肽 S 转移酶基因会导致花色变淡。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0409a

Misako Akagi, Noriko Nakamura, Yoshikazu Tanaka

引用次数: 0

Rice KORPOKKUR gene is expressed in mitotic cells and regulates pleiotropic features during vegetative phase. 水稻 KORPOKKUR 基因在有丝分裂细胞中表达，并在无性繁殖期调节多生物特征。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0305a

Kaito Chiba, Takumi Tezuka, Hiroetsu Wabiko, Yasuo Nagato, Nobuhiro Nagasawa, Namiko Satoh-Nagasawa

引用次数: 0

The blue light signaling inhibitor 3-bromo-7-nitroindazole affects gene translation at the initial reception of blue light in young Arabidopsis seedlings. 蓝光信号抑制剂 3-bromo-7-nitroindazole 会影响拟南芥幼苗最初接受蓝光时的基因翻译。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0323a

Yukio Kurihara, Chika Akagi, Yuko Makita, Masaharu Kawauchi, Emiko Okubo-Kurihara, Tomohiko Tsuge, Takashi Aoyama, Minami Matsui

{"title":"The blue light signaling inhibitor 3-bromo-7-nitroindazole affects gene translation at the initial reception of blue light in young Arabidopsis seedlings.","authors":"Yukio Kurihara, Chika Akagi, Yuko Makita, Masaharu Kawauchi, Emiko Okubo-Kurihara, Tomohiko Tsuge, Takashi Aoyama, Minami Matsui","doi":"10.5511/plantbiotechnology.24.0323a","DOIUrl":"10.5511/plantbiotechnology.24.0323a","url":null,"abstract":"Initial light reception after germination is a dramatic life event when a seedling starts proper morphogenesis. Blue light contains a range of light wavelengths that plants can perceive. A previous report suggested that the chemical compound 3-bromo-7-nitroindazole (3B7N) inhibits blue light-mediated suppression of hypocotyl elongation by physically interacting with the blue light receptor Cryptochrome 1 (CRY1). We previously examined changes of genome-wide gene expression in Arabidopsis seedlings germinated in the dark and then exposed to blue light by RNA-seq and Ribo-seq analyses. The expression of ribosome-related genes was translationally upregulated in response to the initial blue light exposure, depending on signals from both the nucleus and chloroplasts. Here, we re-analyzed our previous data and examined the effect of 3B7N treatment on changes in gene expression upon blue light exposure. The results showed that 3B7N negatively affected translation of ribosome-related genes and, interestingly, the effects were similar to not only those in cry1cry2 mutants but also plants under suppression of photosynthesis. We propose an apparent crosstalk between chloroplast function and blue light signaling.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eugenol transport and biosynthesis through grafting in aromatic plants of the Ocimum genus. 欧芹属芳香植物通过嫁接进行的丁香酚运输和生物合成。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0124a

Shogo Hirose, Kaito Sakai, Sawa Kobayashi, Masato Tsuro, Atsushi Morikami, Hironaka Tsukagoshi

{"title":"Eugenol transport and biosynthesis through grafting in aromatic plants of the Ocimum genus.","authors":"Shogo Hirose, Kaito Sakai, Sawa Kobayashi, Masato Tsuro, Atsushi Morikami, Hironaka Tsukagoshi","doi":"10.5511/plantbiotechnology.24.0124a","DOIUrl":"10.5511/plantbiotechnology.24.0124a","url":null,"abstract":"Aromatic compounds play essential roles in plant physiology and various industries because of their unique fragrances and beneficial properties. In this study, we investigated the transport and biosynthesis of eugenol, a prominent aromatic compound, within the Ocimum genus, using grafting experiments. Grafting sweet basil (Ocimum basilicum) scions onto diverse rootstocks, including tobacco (Nicotiana benthamiana) and thyme (Thymus vulgaris), revealed that eugenol is transported from the shoot to the root across distinct plant species. Furthermore, grafting within the Ocimum genus, which includes O. basilicum, O. tenuiflorum, and O. americanum, resulted in variations in eugenol transport and accumulation. The eugenol content in the shoots remained constant across all combinations, whereas the root eugenol levels varied depending on the scion-rootstock pair. To elucidate the biosynthetic capabilities of eugenol in Ocimum roots, we performed in vitro enzyme assays using crude protein extracts from roots, which revealed that eugenol can be synthesized in roots in addition to being transported. Expression analysis of eugenol synthase (EGSs) genes showed that EGS4 expression was influenced by grafting in O. basilicum roots, suggesting compensation by other EGSs. Our results suggest that eugenol transport and biosynthesis are multifaceted processes influenced by the interactions between different species and tissues. The potential to engineer eugenol levels in rootstocks lacking biosynthetic capacity has potential applications in agriculture and industry. This study reveals the dynamic interplay between eugenol transport and biosynthesis in the Ocimum genus, providing insights into the manipulation of aromatic compound production in plants.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and characterization of immobilized thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds on DEAE-cellulose and chitosan bead for operational stability. 从发芽剑豆（Canavalia gladiata (Jacq.) DC.）种子中提取的固定化恒温α-淀粉酶在 DEAE-纤维素和壳聚糖珠上的优化和表征，以确保其操作稳定性。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0326a

Saijai Posoongnoen, Sutthidech Preecharram, Jinda Jandaruang, Theera Thummavongsa

{"title":"Optimization and characterization of immobilized thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds on DEAE-cellulose and chitosan bead for operational stability.","authors":"Saijai Posoongnoen, Sutthidech Preecharram, Jinda Jandaruang, Theera Thummavongsa","doi":"10.5511/plantbiotechnology.24.0326a","DOIUrl":"10.5511/plantbiotechnology.24.0326a","url":null,"abstract":"Thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds has been successfully immobilized on DEAE-cellulose (ICgAmy1) and chitosan bead (ICgAmy2) support materials. Optimum conditions of immobilization for DEAE-cellulose and chitosan bead revealed 97% and 96% immobilization yield, respectively. The optimum pH and temperature of both DEAE-cellulose and chitosan bead immobilized α-amylases were pH 7 and 70°C. Both ICgAmy1 and ICgAmy2 were high stability over a wide pH range of pH 5-9 and a temperature range of 70-90°C. In addition, ICgAmy1 and ICgAmy2 led to an operationally stable biocatalyst with above 74% and 76% residual activity after 10 reuses, respectively. Immobilized α-amylases showed high storage stability with 81% (ICgAmy1) and 85% (ICgAmy2) residual activity after 120 days of storage. The easy immobilization process on low-cost, biodegradable, and renewable support materials exhibited an increase in the enzyme operation range and storage stability which reduces production costs. This makes immobilized amylases an effective biocatalyst in various industrial applications especially a potential candidate for bioethanol production, a key renewable energy source.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research advances in regulation and genetic engineering of floral scents. 花香调控和基因工程方面的研究进展。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0312a

Takao Koeduka

引用次数: 0

Comparison of functional properties of unripe papaya fruits of different sexes. 比较不同性别未成熟木瓜果实的功能特性。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0421a

Kota Kera, Haruka Asada, Shunsuke Kikuchi, Shoma Saito, Masumi Iijima, Tsutomu Nakayama, Hideyuki Suzuki

{"title":"Comparison of functional properties of unripe papaya fruits of different sexes.","authors":"Kota Kera, Haruka Asada, Shunsuke Kikuchi, Shoma Saito, Masumi Iijima, Tsutomu Nakayama, Hideyuki Suzuki","doi":"10.5511/plantbiotechnology.24.0421a","DOIUrl":"10.5511/plantbiotechnology.24.0421a","url":null,"abstract":"Papaya (Carica papaya L.) is a herbaceous plant belonging to the family Caricaceae in the order Brassicales. The shape of papaya fruit was linked to sex, and the fruit of female plants is round, whereas that of hermaphrodites is pyriform. Although fruit shape preferences vary by region, differences in their functionalities have not been investigated. Since unripe fruit, also called green papaya, is known for its nutritional and therapeutic benefits, we performed a metabolome analysis of unripe papaya using liquid chromatography coupled with quadrupole/time of flight mass spectrometry. We first focused on capraine derivatives, major piperidine alkaloids, and bioactive compounds with significant antiplasmodial activity. Interestingly, carpaine derivatives tended to be altered in the peel and pulp but not in the seed. Multivariate analyses indicated little difference or minor differences to the extent that they can be caused by individual differences in metabolite profiling between the two sexes. Conversely, total polyphenol content and proteolytic activity were also investigated, but there were no differences between females and hermaphrodites for total polyphenol content and proteolytic activity. In conclusion, the metabolome and major functionalities were similar between hermaphrodites and female unripe fruit. However, it would be worth considering the sex of the material fruit, especially when focusing on the functional properties of carpaine derivatives.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0