Plant Biotechnology最新文献

A simple SmR selectable marker gene for Streptomycin/Spectinomycin selection in Arabidopsis nuclear transformation. 拟南芥核转化中链霉素/大观霉素选择的简单SmR选择标记基因。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0420b

Aoha Miki, Shunya Hirata, Juna Akiyama, Mana Shimatani, Kappei Kobayashi, Hidetaka Kaya

{"title":"A simple SmR selectable marker gene for Streptomycin/Spectinomycin selection in Arabidopsis nuclear transformation.","authors":"Aoha Miki, Shunya Hirata, Juna Akiyama, Mana Shimatani, Kappei Kobayashi, Hidetaka Kaya","doi":"10.5511/plantbiotechnology.26.0420b","DOIUrl":"10.5511/plantbiotechnology.26.0420b","url":null,"abstract":"Selectable marker genes are essential for recovering rare transformants during plant genetic transformations. However, only a limited number of markers are routinely used in Arabidopsis thaliana, particularly for nuclear transformation by the floral dip method. Aminoglycoside-3″-adenylyltransferase (AadA) has been widely used in plastid transformation to confer resistance to Streptomycin and Spectinomycin; however, its applicability to nuclear transformation in Arabidopsis has not been systematically examined. Here, we evaluated an Arabidopsis-codon-optimized AadA1 (Streptomycin/Spectinomycin Resistance: SmR) gene derived from Escherichia coli and lacking any plastid-targeting sequence, as a selectable marker for Arabidopsis nuclear transformation. Dose-response analyses revealed consistent Streptomycin and Spectinomycin sensitivity profiles across the four accessions (Col, L. er, Ws, and C24). When introduced via Agrobacterium-mediated floral dip, SmR conferred robust resistance to 50 mg l-1 Streptomycin or 10 mg l-1 Spectinomycin, enabling clear visual discrimination between resistant transformants and bleached non-transformants. Segregation analyses of T2 progeny revealed Mendelian 3 : 1 ratios, indicating successful transformation of the nuclear genome. Importantly, SmR transformants remained fully sensitive to Kanamycin and Hygromycin, demonstrating that SmR does not confer cross-resistance to these commonly used antibiotics. This compatibility enables the simultaneous use of Spectinomycin, Kanamycin, and Hygromycin for triple selection, allowing efficient isolation of triple transgenic plants. These results establish the non-targeted SmR as an efficient and cost-effective selectable marker for Arabidopsis nuclear transformation and expand the practical repertoire of plant selectable marker systems.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 2","pages":"233-236"},"PeriodicalIF":1.4,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13324387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-Aminobutyric acid enhances Arabidopsis thaliana resistance to the hemibiotrophic fungal pathogen Colletotrichum higginsianum. β-氨基丁酸增强拟南芥对半生物营养真菌病原菌炭疽菌的抗性。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0119a

Mari Narusaka, Yoshihiro Narusaka

{"title":"β-Aminobutyric acid enhances Arabidopsis thaliana resistance to the hemibiotrophic fungal pathogen Colletotrichum higginsianum.","authors":"Mari Narusaka, Yoshihiro Narusaka","doi":"10.5511/plantbiotechnology.26.0119a","DOIUrl":"10.5511/plantbiotechnology.26.0119a","url":null,"abstract":"β-Aminobutyric acid (BABA) is a nonproteinogenic amino acid that functions as a potent plant defense activator, conferring broad-spectrum resistance in many plants. Although its effects on biotrophic and necrotrophic pathogens are well known, the molecular basis of BABA-induced resistance (BABA-IR) against the hemibiotrophic fungus Colletotrichum higginsianum remains unclear. In this study, we investigated BABA's effects on C. higginsianum-infected Arabidopsis thaliana. Foliar BABA application (1.25-10 mM) reduced lesion size and fungal biomass in a concentration-dependent manner, with 5-10 mM being most effective. Microscopy showed that BABA treatment prevented fungal invasion beyond the appressorium stage. Mutant analysis revealed that BABA-IR depends on salicylic acid (SA) biosynthesis (NahG and eds16-1) and the signaling components EDS1 and NDR1. In contrast, mutants insensitive to jasmonic acid and ethylene (jar1-1 and ein2-12, respectively) retained BABA-mediated protection. These findings indicate that BABA primes SA-dependent defenses against C. higginsianum while highlighting its potential for improving sustainable crop protection.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 2","pages":"221-226"},"PeriodicalIF":1.4,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13324208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A curated list of InDel markers for rapid genetic mapping in Arabidopsis thaliana. 拟南芥InDel标记的快速遗传定位。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0420a

Fahad Mohammed Tonmoy Chowdhury, Koki Mutsuda, Taku Takahashi

引用次数: 0

Identification of different cDNA clones of the Apomixis-specific gene-1 homolog involved in aposporous embryo sac formation in guineagrass (Panicum maximum Jacq.). 豚草无孢子胚囊形成的无融合特异性基因-1同源基因cDNA克隆的鉴定。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0428a

Lanzhuang Chen, Liming Guan, Taiji Adachi

{"title":"Identification of different cDNA clones of the Apomixis-specific gene-1 homolog involved in aposporous embryo sac formation in guineagrass (Panicum maximum Jacq.).","authors":"Lanzhuang Chen, Liming Guan, Taiji Adachi","doi":"10.5511/plantbiotechnology.26.0428a","DOIUrl":"10.5511/plantbiotechnology.26.0428a","url":null,"abstract":"Apomixis is the reproduction mode in which only the mother's genes are transmitted from generation to generation. If this trait can be put to practical use, it is expected to be a truly epoch-making breeding method, as seed production costs can be greatly reduced by fixing F1 hybrids. This study aimed to isolate the aposporous apomixis gene and analyze how the gene(s) will be expressed from/in the aposporous guineagrass (Panicum maximum Jacq.). A new classification method using the ovary length as an index was developed to sample different developmental stages of ovaries and buds in obligate sexual plants and apomicts. A cDNA library was derived from the ovaries of aposporous accession N68/96-8-o-11 staged at the appearance of aposporous initial cells (AICs) to isolate AIC stage-specific genes. Using differential screening, four AIC stage-specific cDNA clones obtained from ten thousand of plaques by Northern blot hybridization showed the same start codon and sequences, ranging in lengths from 577 to 1182 bp. The characteristics and their homologies of the four cDNA clones are similar to Apomixis-specific gene-1 (ASG-1), indicating that they are the different cDNA clones of Apomixis-specific gene-1 homolog (ASG-1H). In situ expression analysis detected signals without distinguishing between ASG-1 mRNA and the ASG-1H on gene expression specifically in AIC, AIC-derived embryo sacs, and root tips and shoot apical meristems of aposporous accession. The finding and identification of ASG-1H expressed at the times of AIC appearance and AIC-derived embryo sac formation, may represent an initial step towards isolating an apospory gene.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 2","pages":"209-219"},"PeriodicalIF":1.4,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13324321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of an isoflavone 6-hydroxylase involved in tectorigenin biosynthesis in kudzu (Pueraria montana var. lobata) and the efficient production of 6-methoxyisoflavones. 葛根6-甲氧基异黄酮合成酶的鉴定及6-甲氧基异黄酮的高效生产。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0330b

Kai Uchida, Masami Yokota Hirai

{"title":"Identification of an isoflavone 6-hydroxylase involved in tectorigenin biosynthesis in kudzu (Pueraria montana var. lobata) and the efficient production of 6-methoxyisoflavones.","authors":"Kai Uchida, Masami Yokota Hirai","doi":"10.5511/plantbiotechnology.26.0330b","DOIUrl":"10.5511/plantbiotechnology.26.0330b","url":null,"abstract":"Kudzu (Pueraria montana var. lobata) produces several isoflavones that have beneficial effects in humans. These isoflavones accumulate in a tissue-specific manner, with 5,7,4'-trihydroxy-6-methoxyisoflavone (tectorigenin) and its derivatives accumulating mainly in flowers. Tectorigenin has various beneficial effects on humans and is in high demand, but its biosynthesis has not been elucidated, making large-scale production difficult. In this study, we elucidated the steps in tectorigenin biosynthesis in kudzu by identifying a novel isoflavone 6-hydroxylase and isoflavone O-methyltransferase through a transcriptome analysis and related enzyme assays. Furthermore, using yeast engineered to express genes encoding these enzymes, we successfully produced the 6-methoxyisoflavones tectorigenin, glycitein, and afrormosin from the inexpensive isoflavones genistein, daidzein, and formononetin, respectively. The tectorigenin production rate was 40 mg l-1 after being cultured for 72 h. These results contribute to large-scale tectorigenin production and to tectorigenin-related research.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 2","pages":"193-199"},"PeriodicalIF":1.4,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13324318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of DELLA or ERECTA suppresses the enlarged phloem phenotype caused by hawaiian skirt in the tomato cv. Micro-Tom. 破坏DELLA或ERECTA可抑制夏威夷裙引起的韧皮部增大表型。Micro-Tom。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0330a

Michael D Thomas, Ping-Wei Chen, Tarek El Mestari, Fabien Lombardo

{"title":"Disruption of DELLA or ERECTA suppresses the enlarged phloem phenotype caused by hawaiian skirt in the tomato cv. Micro-Tom.","authors":"Michael D Thomas, Ping-Wei Chen, Tarek El Mestari, Fabien Lombardo","doi":"10.5511/plantbiotechnology.26.0330a","DOIUrl":"10.5511/plantbiotechnology.26.0330a","url":null,"abstract":"Modulation of plant vascular tissues is of interest for crop improvement because xylem and phloem are responsible for wood production and sugar transport across the plant, respectively. Previous studies have shown that DELLA and ERECTA (ER) regulate vascular tissue development, as their respective mutants exhibit enlarged xylem and disorganized vascular tissue. The tomato hawaiian skirt-1 (hws-1) mutant accumulates microRNAs and displays a strongly enlarged phloem together with slightly delayed xylem development in pedicels. In this study, della and er mutant lines were crossed with hws-1 to evaluate the effect of disrupted DELLA and ER function on the large-phloem phenotype of hws in the resulting double mutants. Measurement of miRNA164 levels, a microRNA that accumulates in hws, revealed that its abundance returned to wild-type levels in the double mutants, indicating that the effects of della and er mutations dominate over hws. Consistently, plant architecture and vascular phenotypes in the double mutants closely resembled those of the della and er single mutants, and the characteristic phloem enlargement of hws was absent. These results indicate that DELLA and ER functions are required for the manifestation of the hws large-phloem phenotype in tomato cv. Micro-Tom and that these genes are largely epistatic to hws for the majority of the morphological traits examined.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 2","pages":"201-208"},"PeriodicalIF":1.4,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13324320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of Arabidopsis BBX11 confers enhanced cold tolerance and alters stress-responsive transcriptional networks. 拟南芥BBX11的过表达增强了其耐寒性，并改变了应激反应转录网络。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0109a

Hong-Yun Chen, Benyapa Kitwetch, Wen-Jin Wang, Kai-Lun Yeh, Ya-Yu Peng, Ichiro Tarashima, Chuan-Ming Yeh

{"title":"Overexpression of Arabidopsis BBX11 confers enhanced cold tolerance and alters stress-responsive transcriptional networks.","authors":"Hong-Yun Chen, Benyapa Kitwetch, Wen-Jin Wang, Kai-Lun Yeh, Ya-Yu Peng, Ichiro Tarashima, Chuan-Ming Yeh","doi":"10.5511/plantbiotechnology.26.0109a","DOIUrl":"10.5511/plantbiotechnology.26.0109a","url":null,"abstract":"B-box (BBX) transcription factors play critical roles in plant acclimation to environmental stresses, yet their involvement in cold stress responses remains incompletely understood. Following a genome-wide analysis of BBX gene expression profiles in Arabidopsis thaliana, we focused on AtBBX11, whose transcript levels increased by up to 80-fold in shoots after 24 h of cold treatment. Co-expression analysis revealed that AtBBX11 is associated with key cold-responsive genes and stress-related pathways, including responses to cold, abscisic acid (ABA), water deprivation, and hypoxia. Functional characterization using overexpression lines demonstrated that AtBBX11 enhances chilling tolerance, as transgenic plants exhibited significantly less reduction in shoot fresh weight compared to wild type under two chilling regimes. Transcriptome analysis under cold stress identified numerous differentially expressed genes, including selected transcription factors from several families, with NAC and bHLH being the most represented. Gene Ontology enrichment highlighted processes related to hypoxia response, phosphate starvation, and ABA signaling. Several transcription factors and hypoxia-responsive genes were further validated by Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR), confirming RNA-seq reliability. Collectively, these findings establish AtBBX11 as a cold-responsive regulator that may modulate multiple stress-related pathways, providing new insights into BBX-mediated mechanisms underlying plant cold tolerance.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 2","pages":"161-173"},"PeriodicalIF":1.4,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13324297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted mutagenesis and base editing using engineered Brevibacillus laterosporus Cas9 with expanded target scope in rice. 扩大水稻靶标范围的工程短芽孢杆菌Cas9的靶向诱变和碱基编辑

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0218a

Katsuya Negishi, Hiroshi Nishimasu, Osamu Nureki, Seiichi Toki, Masaki Endo

{"title":"Targeted mutagenesis and base editing using engineered Brevibacillus laterosporus Cas9 with expanded target scope in rice.","authors":"Katsuya Negishi, Hiroshi Nishimasu, Osamu Nureki, Seiichi Toki, Masaki Endo","doi":"10.5511/plantbiotechnology.26.0218a","DOIUrl":"10.5511/plantbiotechnology.26.0218a","url":null,"abstract":"The CRISPR/Cas9 system is now widely used for precise genome editing in many crop species, allowing targeted mutagenesis and precise base substitution. While CRISPR/Cas9-mediated genome editing is highly accurate, a limitation of this technology is the requirement for an appropriate protospacer adjacent motif (PAM) for target site recognition. Cas9 from Brevibacillus laterosporus (BlCas9) has been reported to recognize N4CNDD PAMs with a pronounced preference for A at the 7th and 8th positions of the PAM, and is used for genome editing in maize and human cells. Recently, the enhanced BlCas9 (enBlCas9) variant was reported that includes two amino acid mutations in the PAM-interacting domain of BlCas9 and exhibits enhanced genome editing activity with an expanded target scope in vitro and in human cells. Here, we demonstrate the BlCas9- and enBlCas9-mediated genome editing in rice. Both BlCas9 and enBlCas9 can introduce targeted mutations in rice, and enBlCas9 can broaden the target scope with a non-A residue at the 7th and 8th bases of the PAM. Furthermore, enBlCas9 is applicable for precise base editing with extended target sites, such as C-to-T and A-to-G base conversions. In addition, we developed and validated a proximal CRISPR targeting method (proxy-CRISPR) in which nuclease-dead SpCas9 (SpdCas9) bound near the target sites can improve the genome editing activity of BlCas9 and enBlCas9. These enBlCas9-based genome editing technologies are expected to broaden the scope of efficient targeted mutation and precise base editing in rice.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 2","pages":"151-160"},"PeriodicalIF":1.4,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13324213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of flavonoid O-diglycoside naringin via sequential glycosylation in engineered Schizosaccharomyces pombe as a whole-cell biocatalyst. 作为全细胞生物催化剂的裂糖酵素序贯糖基化生产类黄酮o -二糖苷柚皮苷。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0125a

Arisa Kubomura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

{"title":"Production of flavonoid O-diglycoside naringin via sequential glycosylation in engineered Schizosaccharomyces pombe as a whole-cell biocatalyst.","authors":"Arisa Kubomura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama","doi":"10.5511/plantbiotechnology.26.0125a","DOIUrl":"10.5511/plantbiotechnology.26.0125a","url":null,"abstract":"Some functions of flavonoid glycosides are determined by the glycan structure or enhanced by increasing the attached sugars. Therefore, engineering the glycan moiety can modify the functions of the flavonoid. We attempted to construct a biotransformation method for producing a flavonoid diglycoside, naringin (NRGI) as a model target, using engineered yeast. Naringenin (NRG) glycosylation is initiated by the regiospecific 7-O-glucosyltransferase (7-O-GlcT) to form naringenin-7-O-glucoside (N7G), followed by further rhamnosylation via the branching-type α1,2-rhamnosyltransferase (1,2RhaT), producing NRGI as the final product. In this study, we introduced three α1,2-rhamnosyl-glucoside synthesis-related enzymes-7-O-GlcT from Arabidopsis thaliana (AtGT-2), 1,2RhaT from Citrus maxima (Cm1,2RhaT), and UDP-Rha synthase from A. thaliana (AtRHM2)-into the fission yeast Schizosaccharomyces pombe. To improve the titer of NRGI, we examined the effects of biotransformation medium composition, initial cell concentration, and cell-permeabilizing reagents. Consequently, we successfully constructed a biotransformation method for producing a flavonoid diglycoside from the aglycone via sequential glycosylation using a single recombinant yeast. Using the optimized biotransformation method, we produced 23.1±1.1 mg l-1 of NRGI, 8.0±0.4% molar conversion, from 136 mg l-1 of NRG in 24 h cultivation. This study demonstrated the first example of flavonoid O-diglycoside production in engineered S. pombe via sequential glycosylation by uridine diphosphate sugar-dependent glycosyltransferases (UGTs) under optimized production conditions.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 2","pages":"181-191"},"PeriodicalIF":1.4,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13324211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of viable regions for successful cryopreservation of orchid protocorms. 兰花原球茎成功低温保存可行区域的评价。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-06-25 DOI: 10.5511/plantbiotechnology.26.0122a

Tomonari Hirano, Ayaka Yamamoto, Hiroka Miyagi, Soichiro Tsuda, Tomohisa Yukawa, Hisato Kunitake

{"title":"Evaluation of viable regions for successful cryopreservation of orchid protocorms.","authors":"Tomonari Hirano, Ayaka Yamamoto, Hiroka Miyagi, Soichiro Tsuda, Tomohisa Yukawa, Hisato Kunitake","doi":"10.5511/plantbiotechnology.26.0122a","DOIUrl":"10.5511/plantbiotechnology.26.0122a","url":null,"abstract":"In Orchidaceae, the protocorm, which forms after seed germination, serves as a crucial genetic resource for plant preservation and breeding. To establish a long-term conservation method for the protocorm, in this study, we developed cryopreservation methods. Protocorms of Bletilla striata, a terrestrial orchid species, were precultured for 3 days in a medium containing 0.3 M sucrose and then cryopreserved using vitrification. Although viable cell staining indicated the presence of many surviving cells in the protocorms, the regrowth rate was extremely low. We categorized the stained regions based on the presence or absence of staining in the shoot apical meristem (SAM). The staining rate in the SAM region was low after cryopreservation, which was considered the cause of the low regrowth rate. To enable cryopreservation of the protocorms, the V-Cryo-plate method was adopted, and the treatment times for the solutions used in dehydration were investigated. Treatment times of 45 min and 2 h for the loading and vitrification solutions were found to be appropriate, respectively. Furthermore, an investigation of the effect of adding plant growth regulators to the preculture medium showed that adding 0.1 µM 1-naphthalene acetic acid resulted in a high SAM survival rate of 88% and regrowth rate of 87%, comparable to that observed with survival cell staining. Therefore, survival in the SAM region is important for regrowth in cryopreserved protocorms. Confirmation of survival regions is an important indicator for bridging the gap between the survival and regrowth rates.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 2","pages":"175-180"},"PeriodicalIF":1.4,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13324209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0