Plant Biotechnology最新文献

{"title":"Integrated gene-free potato genome editing using transient transcription activator-like effector nucleases and regeneration-promoting gene expression by <i>Agrobacterium</i> infection","authors":"Naoyuki Umemoto, Shuhei Yasumoto, Muneo Yamazaki, Kenji Asano, Kotaro Akai, Hyoung Jae Lee, Ryota Akiyama, Masaharu Mizutani, Yozo Nagira, Kazuki Saito, Toshiya Muranaka","doi":"10.5511/plantbiotechnology.23.0530a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.23.0530a","url":null,"abstract":"Genome editing is highly useful for crop improvement. The method of expressing genome-editing enzymes using a transient expression system in Agrobacterium, called agrobacterial mutagenesis, is a shortcut used in genome-editing technology to improve elite varieties of vegetatively propagated crops, including potato. However, with this method, edited individuals cannot be selected. The transient expression of regeneration-promoting genes can result in shoot regeneration from plantlets, while the constitutive expression of most regeneration-promoting genes does not result in normally regenerated shoots. Here, we report that we could obtain genome-edited potatoes by positive selection. These regenerated shoots were obtained via a method that combined a regeneration-promoting gene with the transient expression of a genome-editing enzyme gene. Moreover, we confirmed that the genome-edited potatoes obtained using this method did not contain the sequence of the binary vector used in Agrobacterium. Our data have been submitted to the Japanese regulatory authority, the Ministry of Education, Culture, Sports, Science and Technology (MEXT), and we are in the process of conducting field tests for further research on these potatoes. Our work presents a powerful method for regarding regeneration and acquisition of genome-edited crops through transient expression of regeneration-promoting gene.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135768730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preface to the special issue “Current Status and Future Prospects for the Development of Crop Varieties and Breeding Materials Using Genome Editing Technology”","authors":"Masahiro Nishihara, Toshiya Muranaka","doi":"10.5511/plantbiotechnology.23.0000p","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.23.0000p","url":null,"abstract":"","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135771077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an inducible excision system of a visual marker <i>Ipomoea batatas Myb</i> gene from the genome of transgenic cells.","authors":"Yuka Sato, Mayu Fukuda, Peter Nkachukwu Chukwurah, Tomoko Igawa","doi":"10.5511/plantbiotechnology.23.0309a","DOIUrl":"10.5511/plantbiotechnology.23.0309a","url":null,"abstract":"<p><p>In the plant genetic transformation process, single selection by a chemical-resistant marker gene occasionally allows the proliferation of non-transgenic cells, escaping selection pressure. The additional use of a visual marker gene is effective for accurate selection. For instance, R2R3-MYB genes are used for regulating anthocyanin biosynthesis; however, constitutive <i>Myb</i> expression in transgenic plants is not always desirable and may cause developmental abnormalities due to excess anthocyanin accumulation. To overcome the remaining problems in the use of <i>Myb</i> as a visible marker, we developed T-DNA. <i>Ipomoea batatas</i> <i>Myb</i> (<i>IbMyb</i>) and <i>Cre</i> expression cassettes were inserted between two <i>loxP</i> sequences, and the <i>hygromycin phosphotransferase</i> (<i>HPT</i>) and <i>green fluorescent protein</i> (<i>GFP</i>) expression cassettes were located outside of the <i>loxP</i>-<i>IbMyb</i>-<i>Cre</i>-<i>loxP</i> region. In the developed system, <i>IbMyb</i> and <i>Cre</i> were excised from the genomes of transgenic cells using heat-inducible <i>Cre</i>-<i>loxP</i> recombination. Upon heat treatment in a general incubator, green shoots emerged from purple tobacco transgenic calli that were pigmented with <i>IbMyb</i> expression. The excision of <i>IbMyb</i> from the genome of green shoots was confirmed using polymerase chain reaction (PCR) and sequencing. GFP expression was observed in the roots of the obtained green transgenic plants. We report that the system developed here operated successfully in tobacco, showing the potential to provide an easier and cheaper visual selection of transgenic cells in the genetic transformation process.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45780194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of plant specialized metabolites to modulate soil microbiota.","authors":"Akifumi Sugiyama","doi":"10.5511/plantbiotechnology.23.0227a","DOIUrl":"10.5511/plantbiotechnology.23.0227a","url":null,"abstract":"<p><p>Plant specialized metabolites (PSMs) are considerably diverse compounds with multifaceted roles in the adaptation of plants to various abiotic and biotic stresses. PSMs are frequently secreted into the rhizosphere, a small region around the roots, where they facilitate interactions between plants and soil microorganisms. PSMs shape the host-specific rhizosphere microbial communities that potentially influence plant growth and tolerance to adverse conditions. Plant mutants defective in PSM biosynthesis contribute to reveal the roles of each PSM in plant-microbiota interactions in the rhizosphere. Recently, various approaches have been used to directly supply PSMs to soil by in vitro methods or through addition in pots with plants. This review focuses on the feasibility of the direct PSM application methods to reveal rhizospheric plant-microbiota interactions and discusses the possibility of applying the knowledge gained to future engineering of rhizospheric traits.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47091889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obtainment and confirmation of intergeneric hybrids between marguerite (<i>Argyranthemum frutescens</i> (L.) Sch.Bip.) and two <i>Rhodanthemum</i> species (<i>R. hosmariense</i> (Ball) B. H. Wilcox, K. Bremer & Humphries and <i>R. catananche</i> (Ball) B. H. Wilcox, K. Bremer & Humphries).","authors":"Hiroyuki Katsuoka, Naoya Hamabe, Chiemi Kato, Susumu Hisamatsu, Fujio Baba, Motohiro Taneishi, Toshiyuki Sasaki","doi":"10.5511/plantbiotechnology.23.0202a","DOIUrl":"10.5511/plantbiotechnology.23.0202a","url":null,"abstract":"<p><p><i>Argyranthemum frutescens</i> (L.) Sch.Bip. and <i>Rhodanthemum gayanum</i> (Coss. & Durieu) B. H. Wilcox, K. Bremer & Humphries are capable of hybridization. To expand flower color variation in this intergeneric hybrid group, we performed crosses using <i>A</i>. <i>frutescens</i> as the seed parent and <i>R</i>. <i>hosmariense</i> (Ball) B. H. Wilcox, K. Bremer & Humphries, <i>R. catananche</i> (Ball) B. H. Wilcox, K. Bremer & Humphries as the pollen parent. One plantlet was obtained from each cross between the white to pale pink-flowered <i>A</i>. <i>frutescens</i> and white-flowered <i>R</i>. <i>hosmariense</i>, and from a cross between the pink-flowered <i>A</i>. <i>frutescens</i> and cream to pale yellow-flowered <i>R. catananche</i>, via ovule culture. The cross with <i>R</i>. <i>hosmariense</i> produced an individual with white to pale pink ray florets, and the cross with <i>R</i>. <i>catananche</i> produced an individual with red ray florets. The flower and leaf shape of the progenies was intermediate between the parents, and other morphological traits were also characterized in the same manner. Morphological observations and a cleaved amplified polymorphic sequence marker-based determination, using the internal transcribed spacer region as a target for amplification and the restriction enzyme <i>Afl</i> II, revealed that both individuals are hybrids between <i>A</i>. <i>frutescens</i> and <i>R</i>. <i>hosmariense</i>, <i>R</i>. <i>catananche</i>. To the best of our knowledge, this is the first study to report that crossbreeding between <i>A</i>. <i>frutescens</i> (seed parent) and <i>R</i>. <i>hosmariense</i>, <i>R</i>. <i>catananche</i> (pollen parent) is possible. Moreover, further development of <i>Argyranthemum</i> breeding, especially that of a series of hybrid cultivars with different flower colors, is expected.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42981007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of endophyte diversity of <i>Dendrobium officinale</i> lived on rock and tree.","authors":"Xiaolan Li, Huan Hu, Qunli Ren, Miao Wang, Yimei Du, Yuqi He, Qian Wang","doi":"10.5511/plantbiotechnology.23.0208a","DOIUrl":"10.5511/plantbiotechnology.23.0208a","url":null,"abstract":"<p><p><i>Dendrobium officinale</i> usually lives on rock or tree, but their endophyte diversity has not yet been fully revealed? In this study, high-throughput sequencing technology was used to investigate the endophyte diversity of the roots of <i>D. officinale</i> lived on tree (Group 1-3, arboreal type) and rock (Group 4, lithophytic type). The results showed that their composition of endophytic fungi and bacteria were similar at phylum level, while their relative abundance were different. Their taxa composition and abundance of endophytes differed significantly among groups at the genus level. <i>Alpha</i> diversity of endophytic fungi of lithophytic type was higher than those from arboreal type, while there was no advantage in endophytic bacteria. <i>Beta</i> diversity revealed that the endophytic fungi tended to cluster in each group, but the endophytic bacteria were dispersed among the groups. LEfSe analysis found that the numbers of predicted endophyte biomarkers of lithophytic type were more than arboreal types at genus level, and the biomarkers varied among groups. Microbial network analysis revealed similarities and differences in the taxa composition and abundance of shared and special endophytes in each group. These results suggested that the root endophytes of lithophytic and arboreal <i>D. officinale</i> differed in diversity.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10804140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44522415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Excision of DNA fragments with the <i>piggyBac</i> system in <i>Chrysanthemum morifolium</i>.","authors":"Mitsuko Kishi-Kaboshi, Ayako Nishizawa-Yokoi, Ichiro Mitsuhara, Seiichi Toki, Katsutomo Sasaki","doi":"10.5511/plantbiotechnology.23.0324a","DOIUrl":"10.5511/plantbiotechnology.23.0324a","url":null,"abstract":"<p><p><i>Chrysanthemum morifolium</i> is one of the most popular ornamental plants in the world. However, as <i>C. morifolium</i> is a segmental hexaploid, self-incompatible, and has a sizable heterologous genome, it is difficult to modify its trait systematically. Genome editing technology is one of the attractive methods for modifying traits systematically. For the commercial use of genetically modified <i>C. morifolium</i>, rigorous stabilization of its quality is essential. This trait stability can be achieved by avoiding further genome modification after suitable trait modification by genome editing. Since <i>C. morifolium</i> is a vegetatively propagated plant, an approach for removing genome editing tools is required. In this study, we attempted to use the <i>piggyBac</i> transposon system to remove specific DNA sequences from the <i>C. morifolium</i> genome. Using the luminescence as a visible marker, we demonstrated that inoculation of <i>Agrobacterium</i> harboring hyperactive <i>piggyBac</i> transposase removes inserted 2.6 kb DNA, which harbors <i>piggyBac</i> recognition sequences, from the modified Eluc sequence.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47951222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The construction of an <i>Agrobacterium</i>-mediated transformation system of <i>Gynostemma pentaphyllum</i> using the phosphomannose-isomerase/mannose selection system.","authors":"Muxiu Tan, Fengming Liu, Yueying Xie, Qiaocheng Mo, Fenghua Shi","doi":"10.5511/plantbiotechnology.23.0418a","DOIUrl":"10.5511/plantbiotechnology.23.0418a","url":null,"abstract":"<p><p>In this study, the transformed system mediated by <i>Agrobacterium tumefaciens</i> of <i>Gynostemma pentaphyllum</i> was constructed by using the phosphomannose-isomerase (PMI) gene as a marker. To investigate the cefotaxime sodium salt (Cef) concentration of bacteriostatic medium and the appropriate mannose concentration in the selectable medium, explants of the stems with buds were cultured in a basic medium supplemented with different Cef and mannose concentrations, respectively. After these were optimized, 288 explants were transformed according the protocol described above to verify their availability by using the polymerase chain reaction (PCR), reverse transcription-PCR and chlorophenol red. The results showed that the appropriate Cef concentration for bacteriostatic culture and mannose concentration for selectable culture were 150 mg l^-1 and 3 g l^-1 for stem with buds, respectively. According to the PCR results, the transformation frequency of stems with buds was 20.49% with a regeneration rate of 29.16%. In future, the CPR assay could be the auxiliary method of choice as it is moderately accurate, but it has good maneuverability and is cost effective for large-scale use.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10797527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43812276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene-flow investigation between garden and wild roses planted in close distance","authors":"Yuna Asagoshi, Eri Hitomi, Noriko Nakamura, Seiji Takeda","doi":"10.5511/plantbiotechnology.23.0708a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.23.0708a","url":null,"abstract":"Rose is a major ornamental plant, and a lot of cultivars with attractive morphology, color and scent have been generated by classical breeding. Recent progress of genetic modification produces a novel cultivar with attractive features. In both cases, a major problem is the gene-flow from cultivated or genetically modified (GM) plants to wild species, causing reduction of natural population. To investigate whether gene-flow occurs in wild species, molecular analysis with DNA markers with higher efficient technique is useful. Here we investigated the gene-flow from cultivated roses (Rosa×hybrida) to wild rose species planted in close distance in the field. The overlapping flowering periods and visiting insects suggest that pollens were transported by insects between wild and cultivated roses. We examined the germination ratio of seeds from wild species, and extracted DNA and checked with KSN and APETALA2 (AP2) DNA markers to detect transposon insertions. Using two markers, we successfully detected the outcross between wild and cultivated roses. For higher efficiency, we established a bulking method, where DNA, leaves or embryos were pooled, enabling us to that check the outcross of many plants. Our results suggest that wild species and garden cultivars can cross in close distance, so that they should be planted in distance, and checked the outcross with multiple DNA markers.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135402370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A RING membrane-anchor E3 ubiquitin ligase gene is co-expressed with steroidal glycoalkaloid biosynthesis genes in tomato.","authors":"Tsubasa Shoji,&nbsp;Kazuki Saito","doi":"10.5511/plantbiotechnology.22.1031a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.22.1031a","url":null,"abstract":"<p><p>RING membrane-anchor (RMA) E3 ubiquitin ligases are involved in endoplasmic reticulum (ER)-associated protein degradation, which mediates the regulated destruction of ER-resident enzymes in various organisms. We determined that the transcription factor JASMONATE-RESPONSIVE ETHYLENE RESPONSE FACTOR 4 (JRE4) co-regulates the expression of the RMA-type ligase gene <i>SlRMA1</i>, but not its homolog <i>SlRMA2</i>, with steroidal glycoalkaloid biosynthesis genes in tomato, perhaps to prevent the overaccumulation of these metabolites.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10240918/pdf/plantbiotechnology-39-4-22.1031a.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9591353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}