Plant Biotechnology最新文献_第2页

Enhanced saccharification yields from rice straw by senescence-induced expression of a cytokinin biosynthesis gene in intragenic rice plants. 通过衰老诱导细胞分裂素生物合成基因的表达提高水稻秸秆糖化产量。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.1209b

Kano Miura, Hotaka Nishimura, Yukihiro Ito

引用次数: 0

Production of recombinant human transferrin using transgenic rice cell culture. 利用转基因水稻细胞培养生产重组人转铁蛋白。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.1105b

Arisa Kubomura, Yuki Katayama, Yasuko Matsukura, Hayuma Otsuka, Toshiyuki Saeki, Kanako Sasaki, Ei-Tora Yamamura, Hiroyuki Kajiura, Jong-Kook Lee, Kazuhito Fujiyama, Hiroshi Okawa, Kazuaki Ohara

{"title":"Production of recombinant human transferrin using transgenic rice cell culture.","authors":"Arisa Kubomura, Yuki Katayama, Yasuko Matsukura, Hayuma Otsuka, Toshiyuki Saeki, Kanako Sasaki, Ei-Tora Yamamura, Hiroyuki Kajiura, Jong-Kook Lee, Kazuhito Fujiyama, Hiroshi Okawa, Kazuaki Ohara","doi":"10.5511/plantbiotechnology.25.1105b","DOIUrl":"10.5511/plantbiotechnology.25.1105b","url":null,"abstract":"Transferrin is one of the major soluble serum proteins and is responsible for iron transport. Industrially, it is significant as a component of mammalian cell culture media, where a safe and stable supply is necessary. However, because transferrin is a glycoprotein containing 19 disulfide bonds, it is difficult to produce as a recombinant protein in bacteria, and at present it is mainly sourced from animals. In glycoprotein production, stability of the N-glycan profile is crucial, as glycans play important roles in diverse biological processes and influence the efficacy of glycoproteins. In this study, we aimed to produce recombinant human transferrin (rhTF) with stable N-glycan profiles. We generated transgenic rice calli expressing human TF (hTF) as a secretory glycosylated protein. rhTF was successfully produced as a soluble protein in the liquid culture medium of transgenic rice calli and subsequently purified. We confirmed that rhTF contained two plant-specific N-glycans and that these profiles were consistent across production batches. Purified rhTFs promoted the proliferation of cultured animal cells and human iPS cells, similar to serum-derived transferrin. Our results demonstrate new possibilities for producing recombinant glycoproteins with stable N-glycan profiles using a plant cell culture-based secretory protein expression system.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 1","pages":"73-81"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13170787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA metabolic regulation plays diverse roles in nutrient-dependent seedling growth in Arabidopsis. RNA代谢调控在拟南芥营养依赖性幼苗生长中发挥着多种作用。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.0902a

Kodai Ishibashi, Misato Ohtani

{"title":"RNA metabolic regulation plays diverse roles in nutrient-dependent seedling growth in Arabidopsis.","authors":"Kodai Ishibashi, Misato Ohtani","doi":"10.5511/plantbiotechnology.25.0902a","DOIUrl":"10.5511/plantbiotechnology.25.0902a","url":null,"abstract":"Plants continuously respond to changes in nutrient availability by adjusting their growth and development. However, the role of RNA metabolism in this process is unclear. We performed seedling growth assays using Arabidopsis thaliana mutants defective in RNA metabolism. When grown on full-strength Murashige and Skoog (MS) medium containing 1% sucrose, all mutants had shorter primary roots than the wild type, suggesting the importance of RNA metabolic regulation for seedling growth under nutrient-rich conditions. Primary root growth exhibited distinct responses to the presence of sucrose in the following mutants: ccr4a ccr4b, with defects in the deadenylases CARBON CATABOLITE REPRESSOR4a (CCR4a) and CCR4b, which function in poly(A) tail degradation; mtr4-2, with a mutation in the exosome co-factor mRNA TRANSPORT4 (MTR4), which is required for 3'-5' RNA degradation; and rid1-1, with a defect in the RNA helicase ROOT INITIATION DEFECTIVE1 (RID1), which functions in pre-mRNA splicing. Whereas the ccr4a ccr4b seedlings did not exhibit sucrose-dependent changes in root growth, the mtr4-2 and rid1-1 seedlings exhibited more pronounced growth inhibition in response to a lack of sucrose and reduced MS salt and vitamin concentrations, respectively, compared to the wild type. When grown on 0.1×MS medium without sucrose, upf3-1 seedlings, which lack functional UP-FRAMESHIFT3 (UPF3), a component of nonsense-mediated mRNA decay (NMD), were larger than the wild type, suggesting the importance of NMD in regulating seedling growth under nutrient-limited conditions. Therefore, different RNA metabolic pathways play distinct roles in the nutrient-dependent regulation of plant growth, adjusting plant fitness to different environments.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 1","pages":"133-137"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13170810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient high-quality and high molecular weight plant DNA extraction protocol using Percoll™. 高效、高质量、高分子量植物DNA提取协议使用Percoll™。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.0908a

Kanae Nishii, Michelle L Hart, Nathan Kelso, Sadie Barber, Michael Möller

{"title":"Efficient high-quality and high molecular weight plant DNA extraction protocol using Percoll™.","authors":"Kanae Nishii, Michelle L Hart, Nathan Kelso, Sadie Barber, Michael Möller","doi":"10.5511/plantbiotechnology.25.0908a","DOIUrl":"10.5511/plantbiotechnology.25.0908a","url":null,"abstract":"Next generation long-read sequencing is a powerful approach to generate de novo genome assemblies, however it requires high-quality and high molecular weight (HMW) DNA to reach near-chromosome level assemblies. Some plants are reported as recalcitrant to high-quality HMW DNA extraction due to high levels of secondary metabolites. Streptocarpus schliebenii (Gesneriaceae) is one of those highly recalcitrant plants and its DNA extraction failed repeatedly with previously published protocols. Percoll™ is a silica-based colloid coated with polyvinylpyrrolidone and utilized for various aspects in phase separation including plants' nuclei isolation for Hi-C library and DNA extraction to generate bacterial artificial chromosome clones. In this study, we developed a HMW DNA extraction protocol for long-read sequencing that included a Percoll™ gradient step. To establish a stable protocol, we examined and modified buffers and steps of several previous Percoll™ gradient protocols. Instead of the previously used agarose plug method for Percoll™ DNA extraction, CTAB lysis followed by Qiagen Genomic-Tips was employed. Three Streptocarpus species generated optimal quality and HMW DNA. The method was further tested in 12 species across a wide range of plant lineages. The results were species specific. While HMW DNA was obtained from seven species, HMW and high-quality DNA were obtained from four species, i.e. Iris pseudacorus, Pulmonaria affinis, Corytoplectus speciosus, and Ilex aquifolium. This indicates the wide applicability of this protocol for plants. This protocol provides a useful resource for those who are working on de novo plant genome projects of recalcitrant material to obtain optimal DNA for long-read sequencing.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 1","pages":"139-144"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13170785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing somatic embryogenesis in Indonesian cassava genotypes: Effects of 2,4-D or Picloram supplementation and explant type. 优化印尼木薯基因型的体细胞胚胎发生：添加2,4- d或Picloram和外植体类型的影响。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.1003b

Febriana Dwi Wahyuni, Ahmad Fathoni, Dewi Sukma, Sintho Wahyuning Ardie, Ima Mulyama Zainuddin, Baoxiu Qi, Sudarsono Sudarsono

{"title":"Optimizing somatic embryogenesis in Indonesian cassava genotypes: Effects of 2,4-D or Picloram supplementation and explant type.","authors":"Febriana Dwi Wahyuni, Ahmad Fathoni, Dewi Sukma, Sintho Wahyuning Ardie, Ima Mulyama Zainuddin, Baoxiu Qi, Sudarsono Sudarsono","doi":"10.5511/plantbiotechnology.25.1003b","DOIUrl":"10.5511/plantbiotechnology.25.1003b","url":null,"abstract":"Modern biotechnological approaches in technology for cassava propagation depend on somatic embryogenic calli (SEC) induction and somatic embryo (SE) regeneration techniques. Consequently, it is essential to develop SEC induction and SE regeneration for cassava genotypes. By assessing the effects of different auxins and explant types, this study aims to develop efficient techniques for inducing cassava SEC and SE. Callus induction medium (CIM) supplemented with 10 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) or 12 mg l-1 Picloram was used to cultivate five different types of cassava explants. Subsequently, the induced calli were maintained on CIM until the formation of SEC, which were sub-cultured on the same CIM to promote proliferation and induce SE formation. These SEs subsequently germinated and developed into shoots, which were rooted to produced complete plantlets . These findings indicate that culturing-induced axillary bud explants on CIM supplemented with 12 mg l-1 Picloram is an effective method for inducing SEC in cassava. Histological analyses and cryogenic electron microscopy observations confirmed the development of SECs and SEs on CIM. Our finding, in which up to 202.3 SEs were regenerated from 10 induced axillary bud explants on CIM supplemented with 12 mg l-1 Picloram in the Menti genotype, highlights the effects of auxin and explant types on SEC and SE induction. Although further research is required, the methods developed in this study will contribute to the successful breeding and micropropagation of cassava.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 1","pages":"27-36"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13170802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fipexide (FPX), a chemical callus inducer, promotes in vitro shoot regeneration and Agrobacterium-mediated genetic transformation in three fruit tree species. Fipexide （FPX）是一种化学愈伤组织诱导剂，可促进三种果树离体茎再生和农杆菌介导的遗传转化。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.1228a

Feng Dai, Masafumi Omori, Ryutaro Tao

{"title":"Fipexide (FPX), a chemical callus inducer, promotes in vitro shoot regeneration and Agrobacterium-mediated genetic transformation in three fruit tree species.","authors":"Feng Dai, Masafumi Omori, Ryutaro Tao","doi":"10.5511/plantbiotechnology.25.1228a","DOIUrl":"10.5511/plantbiotechnology.25.1228a","url":null,"abstract":"For fruit trees, inefficient transformation and prolonged juvenile phase lasting for years to decades remain two major bottlenecks for breeding and functional gene analysis. Recently, fipexide (FPX) has been identified as a novel chemical that enhances callus induction, regeneration, and Agrobacterium-mediated transformation. In this study, we aimed to optimize FPX treatment for improving shoot regeneration rate and transformation efficiency in European pear (Pyrus communis), highbush blueberry (Vaccinium corymbosum), and persimmon (Diospyros kaki). Explants were cultured on media supplemented with various concentrations of FPX for different durations. In European pear 'La France', treatment with 3 µM FPX for one week significantly enhanced regeneration compared with the conventional phytohormone condition, whereas higher concentrations (≥10 µM) and extended exposure inhibited regeneration. Similar patterns were observed in European pear 'Bartlett', highbush blueberry 'O'Neal' and persimmon 'Jiro'. Furthermore, 10 µM FPX enhanced Agrobacterium-mediated transient GFP expression in all 3 species. We also attempted to introduce Arabidopsis FLOWERING LOCUS T (FT) into persimmon to shorten juvenile phase and induce precocious flowering. Three transgenic 'Jiro' lines with AtFT overexpression were successfully obtained using 10 µM FPX. Collectively, our findings demonstrate that FPX is a potent enhancer of regeneration and transformation in multiple fruit trees species, offering a promising strategy for accelerating breeding programs and gene function analysis in recalcitrant woody species.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 1","pages":"117-125"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13170794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of parthenocarpy and fruit set through genome editing in tomato variety for processing use. 利用基因组编辑技术增强加工用番茄品种的孤雌结实性和坐果性。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.1003a

Naohiro Koshi, Misaki Kobayashi, Hiroshi Ezura, Kenji Miura

{"title":"Enhancement of parthenocarpy and fruit set through genome editing in tomato variety for processing use.","authors":"Naohiro Koshi, Misaki Kobayashi, Hiroshi Ezura, Kenji Miura","doi":"10.5511/plantbiotechnology.25.1003a","DOIUrl":"10.5511/plantbiotechnology.25.1003a","url":null,"abstract":"Tomatoes are extremely important plants that are cultivated worldwide, with various varieties grown in different regions. The traits required can vary depending on the region and intended use. Parthenocarpy, a trait that confers numerous advantages, reduces the labor required for pollination and minimizes the incidence of poor fruit set owing to temperature fluctuations. Mutations in SlIAA9 induce parthenocarpy in tomatoes, and the introduction of this trait into processed varieties via genome editing suggests its potential to markedly shorten the breeding timeline. Genome editing has gained considerable attention as a breeding technique because it enables precise mutations in specific genes. However, only a few recent studies have reported examples of genome editing in Japanese tomato varieties for processing. In this study, we employed a genome-editing technique targeting SlIAA9 to induce parthenocarpy in the Japanese tomato variety Natsunokoma for processing purposes, thereby reducing the labor required for pollination. The null-segregant Sliaa9 mutant exhibited enhanced parthenocarpy and fruit set. These results suggest that improvements in fruit-bearing and parthenocarpic traits enhance the quality of tomato varieties that are mainly used for processing.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 1","pages":"127-131"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13170819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice resistance to lepidopteran herbivores is enhanced by overexpression of a key transcription factor gene for diterpenoid phytoalexin biosynthesis. 水稻对鳞翅目食草动物的抗性通过过度表达二萜类植物抗菌素生物合成的关键转录因子基因而增强。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.1023a

Yasukazu Kanda, Kazumu Kuramitsu, Akira Takahashi, Mai Tsuda, Yooichi Kainoh, Masaki Mori

{"title":"Rice resistance to lepidopteran herbivores is enhanced by overexpression of a key transcription factor gene for diterpenoid phytoalexin biosynthesis.","authors":"Yasukazu Kanda, Kazumu Kuramitsu, Akira Takahashi, Mai Tsuda, Yooichi Kainoh, Masaki Mori","doi":"10.5511/plantbiotechnology.25.1023a","DOIUrl":"10.5511/plantbiotechnology.25.1023a","url":null,"abstract":"In the midst of a worldwide outbreak of lepidopteran chewing pests such as the fall armyworm (FAW; Spodoptera frugiperda), innovative strategies for pest control are needed. Recent findings have linked the resistance of rice (Oryza sativa) to lepidopteran pests to diterpenoid phytoalexins (DPs), antimicrobial compounds produced in response to disease and abiotic stress conditions. In this study, we explored the involvement of DP biosynthesis-related genes in the response and resistance of rice plants to lepidopteran pests. In interactions between rice and FAW or the oriental armyworm (Mythimna separata), feeding damage from larvae induced the expression of DITERPENOID PHYTOALEXIN FACTOR (DPF), which encodes a key transcription factor in DP biosynthesis, as well as DP biosynthetic genes. Transcriptional analysis suggested that DPF promotes DP biosynthesis in response to chewing by lepidopteran herbivores and other stresses. When lepidopteran larvae were reared on the leaves of DPF-overexpressing transgenic rice plants, which persistently accumulate high DP levels, FAW larvae exhibited poor growth within days. Overexpression of DPF also suppressed the growth of larvae from the oriental armyworm, although the suppression was more moderate. These results demonstrate that DPF overexpression enhances plant resistance to lepidopteran pests, highlighting the potential of DPF as a tool for biotechnological pest control.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 1","pages":"53-59"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13170806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-functional allele of RESTORER OF FERTILITY 4 is functional for the reduction of orf288 RNA in japonica rice. 水稻育性恢复期4号非功能等位基因对orf288 RNA的还原具有一定的功能。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.1116a

Kinya Toriyama, Yuko Iwai, Shinya Takeda, Ayumu Takatsuka, Keisuke Igarashi, Tomohiko Kazama

{"title":"Non-functional allele of RESTORER OF FERTILITY 4 is functional for the reduction of orf288 RNA in japonica rice.","authors":"Kinya Toriyama, Yuko Iwai, Shinya Takeda, Ayumu Takatsuka, Keisuke Igarashi, Tomohiko Kazama","doi":"10.5511/plantbiotechnology.25.1116a","DOIUrl":"10.5511/plantbiotechnology.25.1116a","url":null,"abstract":"Cytoplasmic male sterility (CMS) is driven by the incompatibility between a mitochondrial gene and the nuclear RESTORER OF FERTILITY (Rf) gene. Previously, we identified orf288 as the cryptic CMS-causing gene in the mitochondrial genome of japonica rice (Oryza sativa) Taichung 65 (T65) through the analysis of CMS lines of O. glaberrima carrying the cytoplasm of japonica rice; orf288 RNA was greatly reduced in the presence of a T65-derived nuclear Rf gene, which was linked to a region harboring rf4 encoding a pentatricopeptide repeat (PPR) protein and other Rf-like PPR genes. In this study, we knocked out Rf-like PPR genes in T65 to identify the Rf-like PPR gene regulating the reduction of orf288 RNA and pollen fertility. Among the CRISPR-Cas9-mediated mutations in PPR461 (Os10g0495100), PPR782/rf4 (Os10g0495200), and PPR794 (Os10g0497300), mutations in PPR782/rf4 (Os10g0495200) allowed the expression of orf288; however, the orf288 RNA level was lower than that in the O. glaberrima CMS line and did not affect pollen fertility. Our results indicate that PPR782/rf4, a non-functional allele of the RF4 gene responsible for fertility restoration in wild-abortive (WA)-type CMS, is functional and suppresses the accumulation of orf288 RNA in the japonica rice.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 1","pages":"83-88"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13170784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic expression of a human cysteine aspartate-specific protease gene caspase-3 induces cell death and affects the infection of tomato mosaic virus in Nicotiana plants. 人半胱氨酸-天冬氨酸特异性蛋白酶基因caspase-3异位表达诱导细胞死亡并影响烟草植株中番茄花叶病毒的感染。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2026-03-25 DOI: 10.5511/plantbiotechnology.25.1127a

Yuki Fujii, Seishiro Kato, Kouta Kurihara, Yuta Yamagishi, Eriko Suzuki, Nobumitsu Sasaki, Yasuhiko Matsushita

{"title":"Ectopic expression of a human cysteine aspartate-specific protease gene caspase-3 induces cell death and affects the infection of tomato mosaic virus in Nicotiana plants.","authors":"Yuki Fujii, Seishiro Kato, Kouta Kurihara, Yuta Yamagishi, Eriko Suzuki, Nobumitsu Sasaki, Yasuhiko Matsushita","doi":"10.5511/plantbiotechnology.25.1127a","DOIUrl":"10.5511/plantbiotechnology.25.1127a","url":null,"abstract":"In animals, genes of the cysteine aspartate-specific protease (caspase) family play a crucial role in inducing cell death. Genes homologous to animal caspase genes have not been found in plants, and to date, there are no examples of the ectopic expression of animal caspase genes inducing cell death in plants. In this study, we investigated whether cell death could be induced by expressing the human caspase-3 gene in plants. Wild-type caspase-3 (V266), inactive type (V266H), and constitutively active type (V266E) genes were expressed in leaves of Nicotiana benthamiana and/or Nicotiana tabacum using two types of Agrobacterium-infiltration-type virus vectors and one type of mechanical inoculation-type virus vector. In all cases, cell death symptoms were induced by V266 and V266E but not V266H. Similar results were obtained even when the caspase-3 gene was split into coding regions for subunit 1 and subunit 2 and co-expressed. As for virus infection, expression of V266 and V266E suppressed the infection of the tomato mosaic virus (ToMV). This suppression effect was particularly pronounced when the constitutively active type V266E was expressed using a virus vector of ToMV. With V266E, cell-to-cell movement of the virus was inhibited and long-distance movement did not occur. Furthermore, the expression of V266 and V266E tended to increase the mRNA levels of defense-related genes in N. benthamiana. These results suggest that the expression of the human caspase-3 gene induces cell death in plants, affects plant gene expression, and exerts an inhibitory effect against ToMV infection.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"43 1","pages":"95-103"},"PeriodicalIF":1.4,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13170818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0