Plant Biotechnology最新文献_第9页

Exploring the Roots of the Old GMO Narrative and Why Young People Have Started to Ask Critical Questions 探索旧转基因叙事的根源以及为什么年轻人开始提出关键问题

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2021-01-01 DOI: 10.1007/978-3-030-68345-0_19

P. Aerni

引用次数: 1

Is It Possible to Overcome the GMO Controversy? Some Elements for a Philosophical Perspective 是否有可能克服转基因争议?哲学观点的一些要素

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2021-01-01 DOI: 10.1007/978-3-319-06892-3_9

M. Kuntz

引用次数: 5

Genomic Methods for Improving Abiotic Stress Tolerance in Crops 提高作物非生物抗逆性的基因组方法

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2021-01-01 DOI: 10.1007/978-3-319-06892-3_4

D. Kim, G. Agrawal, R. Rakwal, Shahid Ahmed, J. S. Rohila

引用次数: 1

Policies and Governance for Plant Genome Editing 植物基因组编辑的政策和治理

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2021-01-01 DOI: 10.1007/978-3-030-68345-0_18

J. Schiemann, F. Hartung, Jochen Menz, T. Sprink, R. Wilhelm

引用次数: 1

Sustainable Management of Insect-Resistant Crops 抗虫作物的可持续管理

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2021-01-01 DOI: 10.1007/978-3-030-68345-0_9

S. Fleischer, W. Hutchison, S. Naranjo

引用次数: 2

Temporal restriction of salt inducibility in expression of salinity-stress related gene by the circadian clock in Solanum lycopersicum. 昼夜节律钟对番茄盐胁迫相关基因表达的时间限制。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0703a

K. Coyne, M. M. Davis, T. Mizoguchi, Ryosuke Hayama

{"title":"Temporal restriction of salt inducibility in expression of salinity-stress related gene by the circadian clock in Solanum lycopersicum.","authors":"K. Coyne, M. M. Davis, T. Mizoguchi, Ryosuke Hayama","doi":"10.5511/plantbiotechnology.19.0703a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0703a","url":null,"abstract":"Exposure to salinity causes plants to trigger transcriptional induction of a particular set of genes for initiating salinity-stress responses. Recent transcriptome analyses reveal that expression of a population of salinity-inducible genes also exhibits circadian rhythms. However, since the analyses were performed independently from those with salinity stress, it is unclear whether the observed circadian rhythms simply represent their basal expression levels independently from their induction by salinity, or these rhythms demonstrate the function of the circadian clock to actively limit the timing of occurrence of the salinity induction to particular times in the day. Here, by using tomato, we demonstrate that salt inducibility in expression of particular salinity-stress related genes is temporally controlled in the day. Occurrence of salinity induction in expression of SlSOS2 and P5CS, encoding a sodium/hydrogen antiporter and an enzyme for proline biosynthesis, is limited specifically to the morning, whereas that of SlDREB2, which encodes a transcription factor involved in tomato responses to several abiotic stresses such as salinity and drought, is restricted specifically to the evening. Our findings not only demonstrate potential importance in further investigating the basis and significance of circadian gated salinity stress responses under fluctuating day/night conditions, but also provide the potential to exploit an effective way for improving performance of salinity resistance in tomato.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"195-200"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0703a","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47827325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

A gain-of-function T-DNA insertion mutant of Marchantia polymorpha hyper-accumulates flavonoid riccionidin A. 一个功能获得T-DNA插入突变体的多晶马钱草超积累类黄酮riccionidin A。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0722a

Noriko Hamashima, Xiaonan Xie, Mio Hikawa, Tomohiro Suzuki, Y. Kodama

引用次数: 5

Efficient genome engineering using Platinum TALEN in potato. 马铃薯白金TALEN高效基因组工程研究。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0805a

Shuhei Yasumoto, N. Umemoto, H. Lee, Masaru Nakayasu, Satoru Sawai, Tetsushi Sakuma, Takashi Yamamoto, M. Mizutani, K. Saito, T. Muranaka

{"title":"Efficient genome engineering using Platinum TALEN in potato.","authors":"Shuhei Yasumoto, N. Umemoto, H. Lee, Masaru Nakayasu, Satoru Sawai, Tetsushi Sakuma, Takashi Yamamoto, M. Mizutani, K. Saito, T. Muranaka","doi":"10.5511/plantbiotechnology.19.0805a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0805a","url":null,"abstract":"Potato (Solanum tuberosum) is one of the most important crops in the world. However, it is generally difficult to breed a new variety of potato crops because they are highly heterozygous tetraploid. Steroidal glycoalkaloids (SGAs) such as α-solanine and α-chaconine found in potato are antinutritional specialized metabolites. Because of their toxicity following intake, controlling the SGA levels in potato varieties is critical in breeding programs. Recently, genome-editing technologies using artificial site-specific nucleases such as TALEN and CRISPR-Cas9 have been developed and used in plant sciences. In the present study, we developed a highly active Platinum TALEN expression vector construction system, and applied to reduce the SGA contents in potato. Using Agrobacterium-mediated transformation, we obtained three independent transgenic potatoes harboring the TALEN expression cassette targeting SSR2 gene, which encodes a key enzyme for SGA biosynthesis. Sequencing analysis of the target sequence indicated that all the transformants could be SSR2-knockout mutants. Reduced SGA phenotype in the mutants was confirmed by metabolic analysis using LC-MS. In vitro grown SSR2-knockout mutants exhibited no differences in morphological phenotype or yields when compared with control plants, indicating that the genome editing of SGA biosynthetic genes such as SSR2 could be a suitable strategy for controlling the levels of toxic metabolites in potato. Our simple and powerful plant genome-editing system, developed in the present study, provides an important step for future study in plant science.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"167-173"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0805a","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41865370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Environmental biosafety assessment on transgenic Oncidium orchid modified by RNA interference of Phytoene Synthase genes. 植物烯合酶基因RNA干扰转基因兰菖蒲的环境生物安全性评价。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0814a

Swee-Suak Ko, Yao-Chung Liu, M. Chung, M. Shih, Habibi Mohammadmehdi, Taichi Oguchi, Kazuo N. Watanabe, K. Yeh

{"title":"Environmental biosafety assessment on transgenic Oncidium orchid modified by RNA interference of Phytoene Synthase genes.","authors":"Swee-Suak Ko, Yao-Chung Liu, M. Chung, M. Shih, Habibi Mohammadmehdi, Taichi Oguchi, Kazuo N. Watanabe, K. Yeh","doi":"10.5511/plantbiotechnology.19.0814a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0814a","url":null,"abstract":"Hybrid Oncidium orchids, such as Oncidium Gower Ramsey and Oncidium \"Honey Angel,\" are popular cut flowers in Japan and Taiwan. Due to pollen sterility, no new varieties have been created by conventional breeding methods. Recently, we employed RNA interference (RNAi) technology to suppress phytoene synthase and successfully modified floret hue from yellow to white (Liu et al. 2019). Transgenic white Oncidium orchids, Honey Snow MF-1, have been grown to test their genetic stability, and their environmental biosafety was assessed for approximately one year under government regulatory instructions from the Council of Agriculture, Taiwan. In the present study, pollen sterility was demonstrated by cytological observation of the microsporogenesis step, pollen morphology abortion, and failure of pollen germination. Assays on allelopathic effect on the other plants and the soil rhizospheric microbial flora-revealed that transgenic Oncidium orchids are potentially safe with regard to environmental biodiversity. Therefore, the general release permissions have been granted and an application for licensing for commercial production is under way.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"181-185"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0814a","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41862019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Anise-cultured cells abolish 2,4-dichlorophenoxyacetic acid in culture medium. 大料培养的细胞在培养基中消除2,4-二氯苯氧乙酸。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0805b

Kumiko Tanida, H. Shiota

{"title":"Anise-cultured cells abolish 2,4-dichlorophenoxyacetic acid in culture medium.","authors":"Kumiko Tanida, H. Shiota","doi":"10.5511/plantbiotechnology.19.0805b","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0805b","url":null,"abstract":"In anise (Pimpinella anisum, family Apiaceae), callus-like embryogenic cells (embryogenic callus) are induced by culturing hypocotyl explants in 2,4-dichlorophenoxyacetic acid (2,4-D)-containing medium, and somatic embryos are formed from embryogenic callus transferred into 2,4-D-free medium. Anise somatic embryos are also induced even if embryogenic callus is continually cultured in 2,4-D-containing medium without subculturing. In this study, we aimed to clarify the dynamics of 2,4-D during anise cell culture. After culturing anise callus in 2,4-D-containing medium, 2,4-D in the medium was analyzed by thin-layer chromatography. In the medium, 2,4-D was decreased during anise callus culture, and fully abolished after 5-day culture. On the other hand, no decrease in 2,4-D was observed in the other Apiaceae species (carrot, fennel, dill, parsley, and coriander). After 7-day culture of anise callus, the medium was collected following removal of the cultured cells and 2,4-D was added to the collected medium. After 10 days of incubation and shaking, 2,4-D was markedly decreased in the medium. However, when the collected medium was heat-treated at 100°C, 2,4-D was detected after 20 days of incubation. Therefore, anise callus has a specific 2,4-D degradation system, in which heat-inactivated secreted molecules may participate.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"36 3 1","pages":"209-212"},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0805b","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42226240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1