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Application of Plant Biotechnology 植物生物技术的应用
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2019-01-15 DOI: 10.1201/9780429057267-6
S. Umesha
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引用次数: 0
Molecular Marker-aided Breeding 分子标记辅助育种
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2019-01-15 DOI: 10.1201/9780429057267-5
S. Umesha
{"title":"Molecular Marker-aided Breeding","authors":"S. Umesha","doi":"10.1201/9780429057267-5","DOIUrl":"https://doi.org/10.1201/9780429057267-5","url":null,"abstract":"","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46979787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tissue-culture Applications 组织培养应用
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2019-01-15 DOI: 10.1201/9780429057267-2
S. Umesha
{"title":"Tissue-culture Applications","authors":"S. Umesha","doi":"10.1201/9780429057267-2","DOIUrl":"https://doi.org/10.1201/9780429057267-2","url":null,"abstract":"","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44097938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant Transformation Technology 工厂改造技术
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2019-01-15 DOI: 10.1201/9780429057267-3
S. Umesha
{"title":"Plant Transformation Technology","authors":"S. Umesha","doi":"10.1201/9780429057267-3","DOIUrl":"https://doi.org/10.1201/9780429057267-3","url":null,"abstract":"","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45202031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Important Issues in Plant Biotechnology 植物生物技术中的重要问题
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2019-01-15 DOI: 10.1201/9780429057267-8
S. Umesha
{"title":"Important Issues in Plant Biotechnology","authors":"S. Umesha","doi":"10.1201/9780429057267-8","DOIUrl":"https://doi.org/10.1201/9780429057267-8","url":null,"abstract":"","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46691191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant Genetic Engineering for Productivity and Performance 用于生产力和性能的植物基因工程
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2019-01-15 DOI: 10.1201/9780429057267-4
S. Umesha
{"title":"Plant Genetic Engineering for Productivity and Performance","authors":"S. Umesha","doi":"10.1201/9780429057267-4","DOIUrl":"https://doi.org/10.1201/9780429057267-4","url":null,"abstract":"","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49317110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long-term monitoring of bioluminescence circadian rhythms of cells in a transgenic Arabidopsis mesophyll protoplast culture. 转基因拟南芥叶肉原生质体培养中细胞生物发光昼夜节律的长期监测。
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2018-09-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0515A
Shunji Nakamura, T. Oyama
{"title":"Long-term monitoring of bioluminescence circadian rhythms of cells in a transgenic Arabidopsis mesophyll protoplast culture.","authors":"Shunji Nakamura, T. Oyama","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0515A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0515A","url":null,"abstract":"The circadian system of plants is based on the cell-autonomously oscillating circadian clock. In the plant body, these cellular clocks are associated with each other, but their basic and intrinsic properties are still largely unknown. Here we report a method that enables long-term monitoring of bioluminescence circadian rhythms of a protoplast culture in a complete synthetic medium. From the leaves of Arabidopsis transgenic plants carrying the luciferase gene under a clock-gene promoter, mesophyll protoplasts were isolated and their bioluminescence was automatically measured every 20 min for more than one week. Decreasing luminescence intensities were observed in protoplasts when they were cultured in a Murashige and Skoog-based medium and also in W5 solution. This decrease was dramatically improved by adding the phytohormones auxin and cytokinin to the MS-based medium; robust circadian rhythms were successfully monitored. Interestingly, the period lengths of bioluminescence circadian rhythms of protoplasts under constant conditions were larger than those of detached leaves, suggesting that the period lengths of mesophyll cells in leaves were modulated from their intrinsic properties by the influence of other tissues/cells. The entrainability of protoplasts to light/dark signals was clearly demonstrated by using this monitoring system. By analyzing the circadian behavior of isolated protoplasts, the basic circadian system of plant cells may be better understood.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"291-295"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0515A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49035433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Expression and functional analysis of apple MdMADS13 on flower and fruit formation. 苹果MdMADS13基因在花果形成中的表达及功能分析。
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2018-09-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0510A
M. Wada, H. Oshino, N. Tanaka, N. Mimida, Y. Moriya-Tanaka, C. Honda, T. Hanada, H. Iwanami, S. Komori
{"title":"Expression and functional analysis of apple MdMADS13 on flower and fruit formation.","authors":"M. Wada, H. Oshino, N. Tanaka, N. Mimida, Y. Moriya-Tanaka, C. Honda, T. Hanada, H. Iwanami, S. Komori","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0510A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0510A","url":null,"abstract":"Apple MdMADS13 has a transcription factor with MADS domain. Moreover, it is expressed specifically at petals and carpels. The product forms a dimer with MdPISTILLATA (MdPI) protein as a class B gene for floral organ formation. Reportedly, in parthenocarpic cultivars of apple (Spencer Seedless, Wellington Bloomless, Wickson and Noblow) the MdPI function is lost by genome insertion of retrotransposon, which cultivars show a homeotic mutation of floral organs, petals to sepals and stamens to carpels. Apple fruit is pome from receptacle tissue, and MdSEPALLATA (MdMADS8/9) and AGAMOUS homologues MdMADS15/22 involved in the fruit development, the transgenic apple suppressed these gene showed poor fruit development and abnormal flower formation. This article describes that the MdMADS13 retained expression after blossom and small fruits of parthenocarpic cultivars. Yeast two-hybrid experiment showed specific binding between MdPI and MdMADS13 proteins. Furthermore, transgenic Arabidopsis with 35S::MdMADS13 have malformed stamens and carpels. These results suggest strongly that MdMADS13 is related to flower organ formation as a class B gene with MdPI.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"207-213"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0510A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41555697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Evaluation of internal control genes for quantitative realtime PCR analyses for studying fruit development of dwarf tomato cultivar 'Micro-Tom'. 矮番茄品种“微汤姆”果实发育实时定量PCR分析内控基因的评价
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2018-09-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0525A
Seung-Won Choi, Ken Hoshikawa, S. Fujita, D. Thi, T. Mizoguchi, H. Ezura, Emi Ito
{"title":"Evaluation of internal control genes for quantitative realtime PCR analyses for studying fruit development of dwarf tomato cultivar 'Micro-Tom'.","authors":"Seung-Won Choi, Ken Hoshikawa, S. Fujita, D. Thi, T. Mizoguchi, H. Ezura, Emi Ito","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0525A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0525A","url":null,"abstract":"Quantitative real-time PCR (qRT-PCR) is widely used to analyze the expression profiles of the genes of interest. In order to obtain accurate quantification data, normalization by using reliable internal control genes is essential. In this study, we evaluated the stability and applicability of eight internal control gene candidates for analyzing gene expression during fruit development in dwarf tomato cultivar Micro-Tom. We collected seventeen different samples from flowers and fruits at different developmental stages, and estimated the expression stability of the candidate genes by two statistical algorithms, geNorm and NormFinder. The combined ranking order and qRT-PCR analyses for expression profiles of SlYABBY2a, SlYABBY1a, FRUITFULL1 and APETALA2c suggested that EXPRESSED was the most stable and reliable internal control gene among the candidates. Our analysis also suggested that RPL8 was also suitable if the sample group is limited to fruits at different maturation stages. In addition to EXPRESSED, GAPDH was also applicable for relative quantitation to monitor gene expression profiles through fruit development from pistil to pericarp.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"225-235"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0525A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45283655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Salinity stress-responsive transcription factors in the liverwort Marchantia polymorpha. 多形地茅盐胁迫应答转录因子的研究。
IF 1.6 4区 生物学
Plant Biotechnology Pub Date : 2018-09-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.18.0501A
Hiroyuki Tanaka, R. Suzuki, Nanako Okabe, Tomohiro Suzuki, Y. Kodama
{"title":"Salinity stress-responsive transcription factors in the liverwort Marchantia polymorpha.","authors":"Hiroyuki Tanaka, R. Suzuki, Nanako Okabe, Tomohiro Suzuki, Y. Kodama","doi":"10.5511/PLANTBIOTECHNOLOGY.18.0501A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.18.0501A","url":null,"abstract":"Salinity stress limits plant growth and productivity. To cope with this limitation, the expression patterns of numerous genes are altered in response to salt stress; however, the regulatory mechanisms involved in these changes are unclear. In the present study, we investigated the regulation of the salinity stress response in the liverwort Marchantia polymorpha. The growth of M. polymorpha gemmalings was severely inhibited by NaCl, and RNA-sequencing and quantitative RT-PCR analyses revealed that the expression of several transcription factor gene families was induced by salinity stress. This work provides insight into the molecular mechanisms underlying the salinity stress response in M. polymorpha.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"35 3 1","pages":"281-284"},"PeriodicalIF":1.6,"publicationDate":"2018-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.18.0501A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43781743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
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