Plant Biotechnology最新文献_第7页

Characterization and fine mapping of nonstop glumes 2 (nsg2) mutant in rice (Oryza sativa L.). 水稻不间断颖花2 (nsg2)突变体的鉴定与精细定位。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-09-25 DOI: 10.5511/plantbiotechnology.19.0506a

Yunfeng Li, Xiao-Qin Zeng, Hui Zhuang, Huan Chen, Ting Zhang, Jun Zhang, Hao Zheng, Jun Tang, Hong-Lei Wang, Suxian Ren, Y. Ling, G. He

{"title":"Characterization and fine mapping of nonstop glumes 2 (nsg2) mutant in rice (Oryza sativa L.).","authors":"Yunfeng Li, Xiao-Qin Zeng, Hui Zhuang, Huan Chen, Ting Zhang, Jun Zhang, Hao Zheng, Jun Tang, Hong-Lei Wang, Suxian Ren, Y. Ling, G. He","doi":"10.5511/plantbiotechnology.19.0506a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.19.0506a","url":null,"abstract":"In cereal crops, the grain number per panicle and the grain yield are greatly affected by the number of florets in a spikelet. In wild-type rice, a spikelet only produces one fertile floret and beneath the floret are a pair of sterile lemmas and a pair of rudimentary glumes. This study characterized a rice spikelet mutant nonstop glumes 2 (nsg2). In the nsg2 mutant, both the sterile lemmas and rudimentary glumes were elongated, and part of sterile lemma looked like a lemma in appearance, shape and size. Detailed histological analysis and qPCR analysis revealed that the sterile lemmas in the nsg2 mutant had homeotically transformed into lemma-like organs. This phenotype indicates that NSG2 is involved in the regulation of spikelet development and supports the long-held view that sterile lemmas were derived from the lemmas of the two lateral florets. This implies that the rice spikelet has the potential to be restored to the \"three florets spikelet\", which may have existed in its ancestors. Genetic analysis reveals that the nsg2 trait is controlled by a single recessive gene. The NSG2 gene was finally mapped between markers R-20 and R-39 on chromosome 7 with a physical region of 180 kb. The two MYB family factors LOC_Os07g44030 and LOC_Os07g44090 might be involved in the development of the spikelet and floral organ, and they were considered as candidate genes of NSG2. These results provide a foundation for map-based cloning and function analysis of NSG2, as well as evidence to support \"three-florets spikelet\" breeding in rice.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/plantbiotechnology.19.0506a","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49060690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Measuring plant colors. 测量植物的颜色。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-06-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.19.0322A

Ichiro Kasajima

{"title":"Measuring plant colors.","authors":"Ichiro Kasajima","doi":"10.5511/PLANTBIOTECHNOLOGY.19.0322A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.19.0322A","url":null,"abstract":"Plant colors such as 'green leaf' and 'red apple' are often described based on human sense, even in scientific papers. On the other hand, colors are measured based on colorimetric principles in some papers, especially in the studies of horticultural plants. The science of color measurements ('colorimetry') is not included in any of the popular lectures in schools and universities, thus the principles of color measurements would not be understood by most researchers. The present review will overview the principles of colorimetry, and will introduce colorimetric methods which can be used for scientific measurement of plant colors. That is to say, the reflection spectrum of visible light (380-780 nm) is measured at 5-nm intervals on the surface of leaves or petals in 'Spectrometric Color Measurement' (SCM). The spectral data is multiplied with RGB or XYZ color matching functions and integrated to obtain RGB or XYZ intensities. Alternatively, approximate RGB values are directly obtained in 'Photographic Color Measurement' (PCM). RGB/XYZ intensities are further calculated to obtain 'hue', 'saturation', and 'lightness', the three factors of colors. Colorimetric insights into genetic regulations (such as MYB gene) and physiological regulations (such as alexandrite effect) of plant colors are also described.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.19.0322A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43576237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis. 膜定位泛素连接酶ATL8参与拟南芥糖饥饿反应。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-06-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.19.0328A

Yongming Luo, Shoki Aoyama, Y. Fukao, Y. Chiba, Takeo Sato, J. Yamaguchi

{"title":"Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis.","authors":"Yongming Luo, Shoki Aoyama, Y. Fukao, Y. Chiba, Takeo Sato, J. Yamaguchi","doi":"10.5511/PLANTBIOTECHNOLOGY.19.0328A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.19.0328A","url":null,"abstract":"As major components of the ubiquitin system, ubiquitin ligases mediate the transfer of ubiquitin to specific target substrates, thereby playing important roles in regulating a wide range of cellular processes. The Arabidopsis Tóxicos en Levadura (ATL) family is a group of plant-specific RING-type ubiquitin ligases with N-terminal transmembrane-like domains. To date, 91 ATL isoforms have been identified in the Arabidopsis genome, with some reported to regulate plant responses to environmental stresses. However, the functions of most ATLs remain unclear. This study showed that ATL8 is a sugar starvation response gene and that ATL8 expression was significantly increased by sugar starvation conditions but repressed by exogenous sugar supply. The ATL8 protein was found to possess ubiquitin ligase activity in vitro and to localize to membrane-bound compartments in plant cells. In addition, Starch Synthase 4 was identified as a putative interactor with ATL8, suggesting that ATL8 may be involved in modulating starch accumulation in response to sugar availability. These findings suggest that ATL8 functions as a membrane-localized ubiquitin ligase likely to be involved in the adaptation of Arabidopsis plants to sugar starvation stress.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.19.0328A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47498134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Monitoring autophagy in rice tapetal cells during pollen maturation. 在花粉成熟过程中监测水稻绒毡层细胞的自噬。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-06-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.19.0417A

Shigeru Hanamata, Jumpei Sawada, B. Toh, Seijiro Ono, K. Ogawa, Togo Fukunaga, K. Nonomura, Takamitsu Kurusu, K. Kuchitsu

引用次数: 9

Agroinfiltration-based efficient transient protein expression in leguminous plants. 豆科植物中基于农业浸润的瞬时蛋白高效表达。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-06-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.19.0220B

Takuya Suzaki, Mai Tsuda, H. Ezura, B. Day, K. Miura

{"title":"Agroinfiltration-based efficient transient protein expression in leguminous plants.","authors":"Takuya Suzaki, Mai Tsuda, H. Ezura, B. Day, K. Miura","doi":"10.5511/PLANTBIOTECHNOLOGY.19.0220B","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.19.0220B","url":null,"abstract":"Transient protein expression is an effective tool to rapidly unravel novel gene functions, such as transcriptional activity of promoters and sub-cellular localization of proteins. However, transient expression is not applicable to some species and varieties because of insufficient expression levels. We recently developed one of the strongest agroinfiltration-based transient protein expression systems for plant cells, termed 'Tsukuba system.' About 4 mg/g fresh weight of protein expression in Nicotiana benthamiana was obtained using this system. The vector pBYR2HS, which contains a geminiviral replication system and a double terminator, can be used in various plant species and varieties, including lettuces, eggplants, tomatoes, hot peppers, and orchids. In this study, we assessed the applicability of the Tsukuba system to several species of legumes, including Lotus japonicus, soybean Glycine max, and common bean Phaseolus vulgaris. The GFP protein was transiently expressed in the seedpods of all examined legume species, however, protein expression in leaves was observed only in P. vulgaris. Taken together, our system is an effective tool to examine gene function rapidly in several legume species based on transient protein expression.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.19.0220B","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44198260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Micropropagation of Solanum quitoense var. quitoense by apical bud, petiole and hypocotyl culture. 利用顶芽、叶柄和下胚轴培养进行茄属植物的微繁殖。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-06-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.19.0317A

B. Gutiérrez, M. M. Cobo, M. Orellana, J. Vega, V. Arahana, Viviana Jaramillo, M. Torres

{"title":"Micropropagation of Solanum quitoense var. quitoense by apical bud, petiole and hypocotyl culture.","authors":"B. Gutiérrez, M. M. Cobo, M. Orellana, J. Vega, V. Arahana, Viviana Jaramillo, M. Torres","doi":"10.5511/PLANTBIOTECHNOLOGY.19.0317A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.19.0317A","url":null,"abstract":"The development of in vitro propagation methods can improve the current commercial use and conservation of plants like naranjilla (Solanum quitoense), a distinctive Andean crop and key emerging agricultural product. In the present study, we report in vitro culture protocols for naranjilla apical buds, hypocotyls and petioles. In apical bud culture, MS medium supplemented with 0.10 mg l-1 1-naphtaleneacetic acid (NAA) produced longer plantlets with greater number of leaves. Hypocotyl culture yielded higher number of shoots when using older explants in MS medium supplemented with different combinations of NAA, 6-benzylaminopurine (BAP) and gibberellic acid (GA3). Petiole culture produced a significantly higher number of shoots per explant, with more abundant and bigger leaves, when using MS medium supplemented with 0.02 mg l-1 NAA, 4.50 mg l-1 BAP and 1.00 mg l-1 GA3. A factorial analysis reveals that the interaction between GA3 and NAA/BAP plays an important role in shoot regeneration. These results provide new tools for the in vitro regeneration of naranjilla plants, improving on previously reported protocols for this species by using alternative explant types and regeneration protocols.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.19.0317A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47572531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Aberrant endosperm formation caused by reduced production of major allergen proteins in a rice flo2 mutant that confers low-protein accumulation in grains. 由水稻flo2突变体中主要过敏原蛋白的产生减少引起的胚乳形成异常，该突变体在谷物中的蛋白质积累较低。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-06-25 DOI: 10.5511/PLANTBIOTECHNOLOGY.19.0312A

Hiroshi Teramura, Kyoko Kondo, Masato Suzuki, Hiroaki Kobayashi, Tsutomu Furukawa, Hiroaki Kusano, H. Shimada

{"title":"Aberrant endosperm formation caused by reduced production of major allergen proteins in a rice flo2 mutant that confers low-protein accumulation in grains.","authors":"Hiroshi Teramura, Kyoko Kondo, Masato Suzuki, Hiroaki Kobayashi, Tsutomu Furukawa, Hiroaki Kusano, H. Shimada","doi":"10.5511/PLANTBIOTECHNOLOGY.19.0312A","DOIUrl":"https://doi.org/10.5511/PLANTBIOTECHNOLOGY.19.0312A","url":null,"abstract":"Rice flo2 mutation produces grains showing a reduced amount of storage proteins. Using Nipponbare and the flo2 mutant, we created rice transformants that showed defective production of major allergen proteins RA14 and RA33 (14-16 kDa and 33 kDa allergen proteins, respectively) by RNAi introduction. The knock-down transformant generated using Nipponbare showed greatly reduced accumulation of both allergen proteins, normal growth, and production of a sufficient amount of normal-shaped seeds. F1 seeds were obtained by crossing between the transformants containing RNAi genes to RA14 and RA33, and showed decreased accumulation of both proteins. However, a peculiar phenotype was observed in the flo2 transformants that lacked accumulation of RA14 or RA33. They showed significantly reduced fertility. A wrinkled grain feature was found on the transformant lacking accumulation of RA14. F1 seeds obtained by crossing these transformants showed significantly lower fertility. F2 seeds showed decreases in both allergen proteins but morphological abnormality with small and severely wrinkled features. These results indicated that it is hard to obtain any transformant lacking accumulation of these allergen proteins using the flo2 mutant, whereas a knock-down transformant of both allergen protein genes was obtained when a wild-type Nipponbare was used as a host. These facts strongly suggest that RA14 and RA33 have some roles in rice seeds.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5511/PLANTBIOTECHNOLOGY.19.0312A","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45611009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Molecular Marker-aided Breeding 分子标记辅助育种

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2019-01-15 DOI: 10.1201/9780429057267-5

S. Umesha