Plant Biotechnology最新文献_第7页

Genome editing and molecular analyses of an Arabidopsis transcription factor, LATE FLOWERING. 拟南芥转录因子 LATE FLOWERING 的基因组编辑和分子分析。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2023-12-25 DOI: 10.5511/plantbiotechnology.23.0920a

Yoshimi Nakano, Maki Kawai, Moeca Arai, Sumire Fujiwara

{"title":"Genome editing and molecular analyses of an Arabidopsis transcription factor, LATE FLOWERING.","authors":"Yoshimi Nakano, Maki Kawai, Moeca Arai, Sumire Fujiwara","doi":"10.5511/plantbiotechnology.23.0920a","DOIUrl":"10.5511/plantbiotechnology.23.0920a","url":null,"abstract":"Correct flower organ formation at the right timing is one of the most important strategies for plants to achieve reproductive success. Ectopic overexpression of LATE FLOWERING (LATE) is known to induce late flowering, partly through suppressing expression of the florigen-encoding gene FLOWERING LOCUS T (FT) in Arabidopsis. LATE is one of the C2H2 zinc finger transcription factors, and it has a canonical transcriptional repression domain called the ethylene-responsive element-binding factor-associated amphiphilic repression (EAR) motif at the end of its C terminus. Therefore, LATE is considered a transcriptional repressor, but its molecular function remains unclear. Our genome-edited late mutants exhibited no distinct phenotype, even in flowering, indicating the presence of redundancy from other factors. To reveal the molecular function of LATE and factors working with it, we investigated its transcriptional activity and interactions with other proteins. Transactivation activity assay showed that LATE possesses transcriptional repression ability, which appears to be attributable to both the EAR motif and other sequences. Yeast two-hybrid assay showed the EAR motif-mediated interaction of LATE with TOPLESS, a transcriptional corepressor. Moreover, LATE could also interact with CRABS CLAW (CRC), one of the most important regulators of floral meristem determinacy, through sequences in LATE other than the EAR motif. Our findings demonstrated the possibility that LATE can form a transcriptional repression complex with CRC for floral meristem determinacy.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"40 4","pages":"337-344"},"PeriodicalIF":1.6,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10905564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing genome editing efficiency in wheat: Effects of heat treatments and different promoters for single guide RNA expression 优化小麦基因组编辑效率:热处理和不同启动子对单导RNA表达的影响

4区生物学

Plant Biotechnology Pub Date : 2023-09-25 DOI: 10.5511/plantbiotechnology.23.0717a

Mitsuko Kishi-Kaboshi, Fumitaka Abe, Yoko Kamiya, Kanako Kawaura, Hiroshi Hisano, Kazuhiro Sato

引用次数: 1

Peculiar properties of tuber starch in a potato mutant lacking the α-glucan water dikinase 1 gene GWD1 created by targeted mutagenesis using the CRISPR/dMac3-Cas9 system 缺α-葡聚糖水二激酶1基因的马铃薯突变体块茎淀粉的特性[lt;i>GWD1</i&gt]使用CRISPR/dMac3-Cas9系统进行靶向诱变

4区生物学

Plant Biotechnology Pub Date : 2023-09-25 DOI: 10.5511/plantbiotechnology.23.0823a

Mariko Ohnuma, Kosuke Ito, Karin Hamada, Ami Takeuchi, Kenji Asano, Takahiro Noda, Akira Watanabe, Akiko Hokura, Hiroshi Teramura, Fuminori Takahashi, Hiromi Mutsuro-Aoki, Koji Tamura, Hiroaki Shimada

{"title":"Peculiar properties of tuber starch in a potato mutant lacking the α-glucan water dikinase 1 gene GWD1 created by targeted mutagenesis using the CRISPR/dMac3-Cas9 system","authors":"Mariko Ohnuma, Kosuke Ito, Karin Hamada, Ami Takeuchi, Kenji Asano, Takahiro Noda, Akira Watanabe, Akiko Hokura, Hiroshi Teramura, Fuminori Takahashi, Hiromi Mutsuro-Aoki, Koji Tamura, Hiroaki Shimada","doi":"10.5511/plantbiotechnology.23.0823a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.23.0823a","url":null,"abstract":"Glucose chains in starch are phosphorylated and contribute to structural stabilization. Phosphate groups contained in starch also play a role in retaining moisture. α-Glucan water dikinase 1 (GWD1) is involved in the phosphorylation of glucose chains in starch. In this study, we generated potato mutants of the GWD1 gene using the CRISPR/dMac3-Cas9 system. Observation of the phenotypes of the GWD1-deficient mutants revealed their physiological roles in tuber starch formation. The 4-allele mutants showed growth retardation and a delay in tuber formation. A significant decrease in phosphorus content was detected in the tuber starch of the gwd1 mutant. This mutant starch showed a higher amylose content than the wild-type starch, whereas its gelatinization temperature was slightly lower than that of the WT starch. The peak viscosity of the mutant starch was lower than that of the WT starch. These observations revealed that the starch of the gwd1 mutants had peculiar and unique properties compared to those of WT, sbe3 and gbss1 mutant starches. The amount of tissue-released water due to freeze–thawing treatment was determined on tubers of the gwd1 mutant and compared with those of WT and the other mutants. Significantly less water loss was found in the gwd1, sbe3 and gbss1 mutant tubers than in the WT tubers. Our results indicate that the GWD1 gene is not only important for potato growth, but also largely effective for the traits of tuber starch.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135768731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Recent advances in steroidal glycoalkaloid biosynthesis in the genus Solanum Solanum甾体糖生物碱生物合成研究进展

4区生物学

Plant Biotechnology Pub Date : 2023-09-25 DOI: 10.5511/plantbiotechnology.23.0717b

Ryota Akiyama, Naoyuki Umemoto, Masaharu Mizutani

引用次数: 1

Integrated gene-free potato genome editing using transient transcription activator-like effector nucleases and regeneration-promoting gene expression by Agrobacterium infection 利用瞬时转录激活子样效应核酸酶和农杆菌表达再生促进基因的整合马铃薯无基因基因组编辑感染

4区生物学

Plant Biotechnology Pub Date : 2023-09-25 DOI: 10.5511/plantbiotechnology.23.0530a

Naoyuki Umemoto, Shuhei Yasumoto, Muneo Yamazaki, Kenji Asano, Kotaro Akai, Hyoung Jae Lee, Ryota Akiyama, Masaharu Mizutani, Yozo Nagira, Kazuki Saito, Toshiya Muranaka

{"title":"Integrated gene-free potato genome editing using transient transcription activator-like effector nucleases and regeneration-promoting gene expression by Agrobacterium infection","authors":"Naoyuki Umemoto, Shuhei Yasumoto, Muneo Yamazaki, Kenji Asano, Kotaro Akai, Hyoung Jae Lee, Ryota Akiyama, Masaharu Mizutani, Yozo Nagira, Kazuki Saito, Toshiya Muranaka","doi":"10.5511/plantbiotechnology.23.0530a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.23.0530a","url":null,"abstract":"Genome editing is highly useful for crop improvement. The method of expressing genome-editing enzymes using a transient expression system in Agrobacterium, called agrobacterial mutagenesis, is a shortcut used in genome-editing technology to improve elite varieties of vegetatively propagated crops, including potato. However, with this method, edited individuals cannot be selected. The transient expression of regeneration-promoting genes can result in shoot regeneration from plantlets, while the constitutive expression of most regeneration-promoting genes does not result in normally regenerated shoots. Here, we report that we could obtain genome-edited potatoes by positive selection. These regenerated shoots were obtained via a method that combined a regeneration-promoting gene with the transient expression of a genome-editing enzyme gene. Moreover, we confirmed that the genome-edited potatoes obtained using this method did not contain the sequence of the binary vector used in Agrobacterium. Our data have been submitted to the Japanese regulatory authority, the Ministry of Education, Culture, Sports, Science and Technology (MEXT), and we are in the process of conducting field tests for further research on these potatoes. Our work presents a powerful method for regarding regeneration and acquisition of genome-edited crops through transient expression of regeneration-promoting gene.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135768730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Preface to the special issue “Current Status and Future Prospects for the Development of Crop Varieties and Breeding Materials Using Genome Editing Technology” 专刊《利用基因组编辑技术开发作物品种和选育材料的现状与展望》前言

4区生物学

Plant Biotechnology Pub Date : 2023-09-25 DOI: 10.5511/plantbiotechnology.23.0000p

Masahiro Nishihara, Toshiya Muranaka

引用次数: 0

Gene-flow investigation between garden and wild roses planted in close distance 近距离种植的花园玫瑰与野生玫瑰的基因流动研究

4区生物学

Plant Biotechnology Pub Date : 2023-01-01 DOI: 10.5511/plantbiotechnology.23.0708a

Yuna Asagoshi, Eri Hitomi, Noriko Nakamura, Seiji Takeda

{"title":"Gene-flow investigation between garden and wild roses planted in close distance","authors":"Yuna Asagoshi, Eri Hitomi, Noriko Nakamura, Seiji Takeda","doi":"10.5511/plantbiotechnology.23.0708a","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.23.0708a","url":null,"abstract":"Rose is a major ornamental plant, and a lot of cultivars with attractive morphology, color and scent have been generated by classical breeding. Recent progress of genetic modification produces a novel cultivar with attractive features. In both cases, a major problem is the gene-flow from cultivated or genetically modified (GM) plants to wild species, causing reduction of natural population. To investigate whether gene-flow occurs in wild species, molecular analysis with DNA markers with higher efficient technique is useful. Here we investigated the gene-flow from cultivated roses (Rosa×hybrida) to wild rose species planted in close distance in the field. The overlapping flowering periods and visiting insects suggest that pollens were transported by insects between wild and cultivated roses. We examined the germination ratio of seeds from wild species, and extracted DNA and checked with KSN and APETALA2 (AP2) DNA markers to detect transposon insertions. Using two markers, we successfully detected the outcross between wild and cultivated roses. For higher efficiency, we established a bulking method, where DNA, leaves or embryos were pooled, enabling us to that check the outcross of many plants. Our results suggest that wild species and garden cultivars can cross in close distance, so that they should be planted in distance, and checked the outcross with multiple DNA markers.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135402370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway. 4-苯基丁酸作为一种生长素，通过ibr3独立通路转化为苯乙酸，促进植物再生。

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2022-03-25 DOI: 10.5511/plantbiotechnology.21.1224b

Akira Iwase, Arika Takebayashi, Yuki Aoi, David S. Favero, Shunsuke Watanabe, M. Seo, Hiroyuki Kasahara, K. Sugimoto

{"title":"4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway.","authors":"Akira Iwase, Arika Takebayashi, Yuki Aoi, David S. Favero, Shunsuke Watanabe, M. Seo, Hiroyuki Kasahara, K. Sugimoto","doi":"10.5511/plantbiotechnology.21.1224b","DOIUrl":"https://doi.org/10.5511/plantbiotechnology.21.1224b","url":null,"abstract":"4-Phenylbutyric acid (4PBA) is utilized as a drug to treat urea cycle disorders and is also being studied as a potential anticancer drug that acts via its histone deacetylase (HDAC) inhibitor activity. During a search to find small molecules that affect plant regeneration in Arabidopsis, we found that 4PBA treatment promotes this process by mimicking the effect of exogenous auxin. Specifically, plant tissue culture experiments revealed that a medium containing 4PBA enhances callus formation and subsequent shoot regeneration. Analyses with auxin-responsive or cytokinin-responsive marker lines demonstrated that 4PBA specifically enhances AUXIN RESPONSE FACTOR (ARF)-dependent auxin responses. Our western blot analyses showed that 4PBA treatment does not enhance histone acetylation in Arabidopsis, in contrast to butyric acid and trichostatin A, other chemicals often used as HDAC inhibitors, suggesting this mechanism of action does not explain the observed effect of 4PBA on regeneration. Finally, mass spectroscopic analysis and genetic approaches uncovered that 4PBA in Arabidopsis plants is converted to phenylacetic acid (PAA), a known natural auxin, in a manner independent of peroxisomal IBR3-related β-oxidation. This study demonstrates that 4PBA application promotes regeneration in explants via its auxin activity and has potential applications to not only plant tissue culture engineering but also research on the plant β-oxidation pathway.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"39 1 1","pages":"51-58"},"PeriodicalIF":1.6,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41547470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Near-Isogenic Lines as Powerful Tools to Evaluate the Effect of Individual Phytochemicals on Health and Chronic Diseases 近等基因系作为评估单个植物化学物质对健康和慢性疾病影响的有力工具

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2021-01-01 DOI: 10.1007/978-3-030-68345-0_17

Binning Wu, Jairam K. P. Vanamala, S. Chopra, L. Reddivari

引用次数: 1

New Technologies for Precision Plant Breeding 植物精密育种新技术

IF 1.6 4区生物学

Plant Biotechnology Pub Date : 2021-01-01 DOI: 10.1007/978-3-030-68345-0_4

Shdema Filler-Hayut, C. Melamed-Bessudo, A. Levy

引用次数: 0