{"title":"Integration of co-culture and transport engineering for enhanced metabolite production.","authors":"Yasuyuki Yamada, Miya Urui, Nobukazu Shitan","doi":"10.5511/plantbiotechnology.24.0312b","DOIUrl":null,"url":null,"abstract":"<p><p>Microbial production of valuable plant metabolites is feasible. However, constructing all pathways in a single cell is a formidable challenge, and the extended biosynthetic pathways within cells often result in reduced productivity. To address these challenges, a co-culture system that divides biosynthetic pathways into several host cells and co-cultures has been developed. Various combinations of host cells, along with the optimal conditions for each co-culture, have been documented, leading to the successful production of valuable metabolites. In addition, efficient biosynthesis frequently involves metabolite movement, encompassing substrate uptake, intracellular intermediate transport, and end-product efflux. Recent advances in plant transporters of specialized metabolites have enhanced productivity by harnessing these transporters. This review summarizes the latest findings on co-culture systems and transport engineering and provides insights into the future of valuable metabolite production through the integration of co-culture and transport engineering.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 3","pages":"195-202"},"PeriodicalIF":1.4000,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921134/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.5511/plantbiotechnology.24.0312b","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Microbial production of valuable plant metabolites is feasible. However, constructing all pathways in a single cell is a formidable challenge, and the extended biosynthetic pathways within cells often result in reduced productivity. To address these challenges, a co-culture system that divides biosynthetic pathways into several host cells and co-cultures has been developed. Various combinations of host cells, along with the optimal conditions for each co-culture, have been documented, leading to the successful production of valuable metabolites. In addition, efficient biosynthesis frequently involves metabolite movement, encompassing substrate uptake, intracellular intermediate transport, and end-product efflux. Recent advances in plant transporters of specialized metabolites have enhanced productivity by harnessing these transporters. This review summarizes the latest findings on co-culture systems and transport engineering and provides insights into the future of valuable metabolite production through the integration of co-culture and transport engineering.
期刊介绍：
Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.
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