Integration of co-culture and transport engineering for enhanced metabolite production.

IF 1.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Pub Date : 2024-09-25 DOI:10.5511/plantbiotechnology.24.0312b

Yasuyuki Yamada, Miya Urui, Nobukazu Shitan

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引用次数: 0

Abstract

Microbial production of valuable plant metabolites is feasible. However, constructing all pathways in a single cell is a formidable challenge, and the extended biosynthetic pathways within cells often result in reduced productivity. To address these challenges, a co-culture system that divides biosynthetic pathways into several host cells and co-cultures has been developed. Various combinations of host cells, along with the optimal conditions for each co-culture, have been documented, leading to the successful production of valuable metabolites. In addition, efficient biosynthesis frequently involves metabolite movement, encompassing substrate uptake, intracellular intermediate transport, and end-product efflux. Recent advances in plant transporters of specialized metabolites have enhanced productivity by harnessing these transporters. This review summarizes the latest findings on co-culture systems and transport engineering and provides insights into the future of valuable metabolite production through the integration of co-culture and transport engineering.

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整合共培养和运输工程，以提高代谢物的生产。

微生物生产有价值的植物代谢物是可行的。然而，在单个细胞中构建所有的生物合成途径是一项艰巨的挑战，并且细胞内扩展的生物合成途径往往导致生产力降低。为了解决这些挑战，一种将生物合成途径分成几个宿主细胞和共培养物的共培养系统已经被开发出来。宿主细胞的各种组合，以及每种共培养的最佳条件，已经被记录下来，导致有价值的代谢物的成功生产。此外，有效的生物合成通常涉及代谢物运动，包括底物摄取、细胞内中间体运输和最终产物外排。近年来，植物特化代谢物转运体的研究进展通过利用这些转运体提高了植物的生产力。本文综述了共培养系统和运输工程的最新研究成果，并展望了通过共培养和运输工程的结合生产有价值代谢物的未来。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-PLANT SCIENCES

CiteScore

2.90

自引率

18.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.