Plant Biotechnology最新文献_第5页

Optimization and characterization of immobilized thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds on DEAE-cellulose and chitosan bead for operational stability. 从发芽剑豆（Canavalia gladiata (Jacq.) DC.）种子中提取的固定化恒温α-淀粉酶在 DEAE-纤维素和壳聚糖珠上的优化和表征，以确保其操作稳定性。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0326a

Saijai Posoongnoen, Sutthidech Preecharram, Jinda Jandaruang, Theera Thummavongsa

{"title":"Optimization and characterization of immobilized thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds on DEAE-cellulose and chitosan bead for operational stability.","authors":"Saijai Posoongnoen, Sutthidech Preecharram, Jinda Jandaruang, Theera Thummavongsa","doi":"10.5511/plantbiotechnology.24.0326a","DOIUrl":"10.5511/plantbiotechnology.24.0326a","url":null,"abstract":"Thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds has been successfully immobilized on DEAE-cellulose (ICgAmy1) and chitosan bead (ICgAmy2) support materials. Optimum conditions of immobilization for DEAE-cellulose and chitosan bead revealed 97% and 96% immobilization yield, respectively. The optimum pH and temperature of both DEAE-cellulose and chitosan bead immobilized α-amylases were pH 7 and 70°C. Both ICgAmy1 and ICgAmy2 were high stability over a wide pH range of pH 5-9 and a temperature range of 70-90°C. In addition, ICgAmy1 and ICgAmy2 led to an operationally stable biocatalyst with above 74% and 76% residual activity after 10 reuses, respectively. Immobilized α-amylases showed high storage stability with 81% (ICgAmy1) and 85% (ICgAmy2) residual activity after 120 days of storage. The easy immobilization process on low-cost, biodegradable, and renewable support materials exhibited an increase in the enzyme operation range and storage stability which reduces production costs. This makes immobilized amylases an effective biocatalyst in various industrial applications especially a potential candidate for bioethanol production, a key renewable energy source.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 2","pages":"129-136"},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research advances in regulation and genetic engineering of floral scents. 花香调控和基因工程方面的研究进展。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0312a

Takao Koeduka

引用次数: 0

Improved biolistic transformation and genome editing in wheat by using trehalose for high osmotic treatment. 利用曲哈洛糖进行高渗透处理，改进小麦的生物转化和基因组编辑。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0328a

Chizu Yanagihara, Hiroshi Tsukamoto, Yuji Ishida, Toshihiko Komari

引用次数: 0

Comparison of functional properties of unripe papaya fruits of different sexes. 比较不同性别未成熟木瓜果实的功能特性。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-06-25 DOI: 10.5511/plantbiotechnology.24.0421a

Kota Kera, Haruka Asada, Shunsuke Kikuchi, Shoma Saito, Masumi Iijima, Tsutomu Nakayama, Hideyuki Suzuki

{"title":"Comparison of functional properties of unripe papaya fruits of different sexes.","authors":"Kota Kera, Haruka Asada, Shunsuke Kikuchi, Shoma Saito, Masumi Iijima, Tsutomu Nakayama, Hideyuki Suzuki","doi":"10.5511/plantbiotechnology.24.0421a","DOIUrl":"10.5511/plantbiotechnology.24.0421a","url":null,"abstract":"Papaya (Carica papaya L.) is a herbaceous plant belonging to the family Caricaceae in the order Brassicales. The shape of papaya fruit was linked to sex, and the fruit of female plants is round, whereas that of hermaphrodites is pyriform. Although fruit shape preferences vary by region, differences in their functionalities have not been investigated. Since unripe fruit, also called green papaya, is known for its nutritional and therapeutic benefits, we performed a metabolome analysis of unripe papaya using liquid chromatography coupled with quadrupole/time of flight mass spectrometry. We first focused on capraine derivatives, major piperidine alkaloids, and bioactive compounds with significant antiplasmodial activity. Interestingly, carpaine derivatives tended to be altered in the peel and pulp but not in the seed. Multivariate analyses indicated little difference or minor differences to the extent that they can be caused by individual differences in metabolite profiling between the two sexes. Conversely, total polyphenol content and proteolytic activity were also investigated, but there were no differences between females and hermaphrodites for total polyphenol content and proteolytic activity. In conclusion, the metabolome and major functionalities were similar between hermaphrodites and female unripe fruit. However, it would be worth considering the sex of the material fruit, especially when focusing on the functional properties of carpaine derivatives.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 2","pages":"165-168"},"PeriodicalIF":1.4,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a di-glucose conjugate of 4-hydroxybenzoic acid in bamboo cells expressing bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase. 在表达细菌 4-hydroxycinnamoyl-CoA hydratase/lyase 的竹细胞中鉴定 4-hydroxybenzoic acid 的二葡萄糖共轭物。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-03-25 DOI: 10.5511/plantbiotechnology.23.1218a

Naoki Ube, Yasuo Kato, Taiji Nomura

{"title":"Identification of a di-glucose conjugate of 4-hydroxybenzoic acid in bamboo cells expressing bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase.","authors":"Naoki Ube, Yasuo Kato, Taiji Nomura","doi":"10.5511/plantbiotechnology.23.1218a","DOIUrl":"10.5511/plantbiotechnology.23.1218a","url":null,"abstract":"Rational metabolic-flow switching is an effective strategy that we proposed for producing exogenous high-value natural products using transformed plant cells. In an earlier proof-of-concept study, we generated bamboo (Phyllostachys nigra; Pn) cells expressing the 4-hydroxycinnamoyl-CoA hydratase/lyase gene of Pseudomonas putida KT2440 (PpHCHL). The encoded enzyme catalyzes the formation of 4-hydroxybenzaldehyde and vanillin from p-coumaroyl-CoA and feruloyl-CoA, respectively. The PpHCHL-transformed Pn cells accumulated mono-glucose conjugates (glucoside and glucose ester) of 4-hydroxybenzoic acid and vanillic acid, indicating that the products (aldehydes) of the PpHCHL-catalyzed reaction were oxidized by endogenous enzyme(s) in Pn cells. In this study, we re-examined the extracts of PpHCHL-transformed Pn cells to screen for additional 4-hydroxybenzoic acid derivatives. An unidentified compound was detected exclusively in the PpHCHL-transformed Pn cells. This compound was purified via column chromatography and then identified as a di-glucose conjugate of 4-hydroxybenzoic acid (i.e., β-D-glucopyranosyl 4-O-β-D-glucopyranosylbenzoate), implying that some of the mono-glucose conjugates of 4-hydroxybenzoic acid were converted to the di-glucose conjugate by endogenous enzyme(s) in Pn cells. The maximum production titer of this di-glucose conjugate in the suspension-cultured cells was 0.38 g l-1, which was the second highest titer among the four glucose conjugates produced by the PpHCHL-transformed Pn cells. The study findings further support the utility of PpHCHL-transformed Pn cells for the bioproduction of 4-hydroxybenzoic acid and its derivatives.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 1","pages":"83-87"},"PeriodicalIF":1.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SHOOT GRAVITROPISM 5 mediates the stomatal response to darkness in Arabidopsis. 拟南芥的 SHOOT GRAVITROPISM 5 介导了气孔对黑暗的反应。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-03-25 DOI: 10.5511/plantbiotechnology.23.1122a

Moeca Arai, Keiko Kigoshi, Kosuke Moriwaki, Kyoko Miyashita, Yoshimi Nakano, Sumire Fujiwara

{"title":"SHOOT GRAVITROPISM 5 mediates the stomatal response to darkness in Arabidopsis.","authors":"Moeca Arai, Keiko Kigoshi, Kosuke Moriwaki, Kyoko Miyashita, Yoshimi Nakano, Sumire Fujiwara","doi":"10.5511/plantbiotechnology.23.1122a","DOIUrl":"10.5511/plantbiotechnology.23.1122a","url":null,"abstract":"Stomatal regulation, a multifaceted mechanism enabling plants to adapt to diverse environmental conditions and optimize photosynthesis for survival and growth, is considered crucial in drought stress tolerance research. To further enhance our understanding of stomatal regulation, we investigated the novel transcription factors involved in this process. Our findings reveal that SHOOT GRAVITROPISM 5 (SGR5) is involved in the stomatal response to darkness in Arabidopsis. Water loss measurements showed that SGR5-overexpressing plants retained more water, whereas SGR5-knockout lines exhibited increased water loss compared with the control. Unexpectedly, our analyses indicated that SGR5 was not associated with the abscisic acid signaling pathway, in contrast to its homologous transcription factor, INDETERMINATE DOMAIN 14. Instead, SGR5-knockout lines exhibited weakened stomatal closure responses upon transition to darkness. Collectively, our results highlight the regulatory role of SGR5 in mediating stomatal movement in response to darkness.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 1","pages":"19-25"},"PeriodicalIF":1.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular cloning and biochemical characterization of indole-3-acetic acid methyltransferase from Japanese star anise (Illicium anisatum). 日本八角茴香（Illicium anisatum）中吲哚-3-乙酸甲基转移酶的分子克隆和生物化学特征。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-03-25 DOI: 10.5511/plantbiotechnology.23.1224a

Takao Koeduka, Ako Nakabo, Ami Takata, Ryo Ikeda, Hideyuki Suzuki, Sakihito Kitajima, Shin-Ichi Ozaki

{"title":"Molecular cloning and biochemical characterization of indole-3-acetic acid methyltransferase from Japanese star anise (Illicium anisatum).","authors":"Takao Koeduka, Ako Nakabo, Ami Takata, Ryo Ikeda, Hideyuki Suzuki, Sakihito Kitajima, Shin-Ichi Ozaki","doi":"10.5511/plantbiotechnology.23.1224a","DOIUrl":"10.5511/plantbiotechnology.23.1224a","url":null,"abstract":"SABATH proteins methylate the carboxyl groups or nitrogen atoms of small plant molecules and play important roles in many developmental processes and plant defense responses. Previous studies have shown that indole-3-acetic acid (IAA) carboxyl methyltransferase (IAMT), a member of the SABATH methyltransferase family, converts IAA into its methyl ester (Me-IAA). We used RNA-seq analysis to identify a putative IAMT gene, IaIAMT, in the ancient angiosperm Illicium anisatum. Functional characterization of the recombinant IaIAMT protein expressed in Escherichia coli showed the highest level of activity with IAA, whereas indole-3-propionic acid and indole-3-butyric acid were not used as substrates. The apparent Km value of IaIAMT using IAA as a substrate was determined to be 122 µM. Phylogenetic analysis and structural modeling of IaIAMT suggested that IaIAMT evolved independently from IAMTs isolated from other plant species, whereas strict substrate specificity toward IAA was conserved in Illicium species, as observed in other plants.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 1","pages":"65-70"},"PeriodicalIF":1.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roots applicable, high sensitivity and specificity assay for the detection of Candidatus Liberibacter asiaticus in citrus roots and fruits. 适用于根部的高灵敏度和特异性检测方法，用于检测柑橘根部和果实中的亚洲自由杆菌。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-03-25 DOI: 10.5511/plantbiotechnology.23.1129a

Zecheng Zhong, Yu Chen, Jinhua Liu, Wei Wang, Feng Zhou, Liu Hu, Jinlian Zhang, Tingsu Chen, Jiyu Xiang, Tingdong Li, Yingbin Wang, Shiyin Zhang, Shengxiang Ge, Jun Zhang, Ningshao Xia

{"title":"Roots applicable, high sensitivity and specificity assay for the detection of Candidatus Liberibacter asiaticus in citrus roots and fruits.","authors":"Zecheng Zhong, Yu Chen, Jinhua Liu, Wei Wang, Feng Zhou, Liu Hu, Jinlian Zhang, Tingsu Chen, Jiyu Xiang, Tingdong Li, Yingbin Wang, Shiyin Zhang, Shengxiang Ge, Jun Zhang, Ningshao Xia","doi":"10.5511/plantbiotechnology.23.1129a","DOIUrl":"10.5511/plantbiotechnology.23.1129a","url":null,"abstract":"Candidatus Liberibacter asiaticus (CLas), a phloem-limited Gram-negative bacterium, is associated with citrus huanglongbing (HLB), which is one of the most destructive diseases currently threatening citrus production worldwide. No effective treatment for HLB is currently available. Effective prevention and control in the initial stage can block the spread and disease progression of HLB. Herein, we developed a co-detection assay for the 16S rDNA and 16S rRNA of CLas, the sensitivity of the co-detection assay was significantly increased over that of the single CLas DNA detection system. Beyond this, we found that the co-detection assay was a better fit to the root samples with higher population abundance than the previous reported detection system because it has a better specificity. Moreover, we found that the contents of 16S rRNA of CLas in citrus roots and fruits are significantly higher than that in leaves, which suggests that the time of HLB diagnosis is probably earlier by using these special tissues and the replication of CLas may become more active in these tissues, further suggested that the significance of study the mechanism of infection, prevention and control of HLB staring from these tissues.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 1","pages":"27-34"},"PeriodicalIF":1.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drought stress induces early flowering and the stress tolerance of offspring in Petunia hybrida. 干旱胁迫诱导矮牵牛杂交种提前开花并提高其后代的抗逆性。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-03-25 DOI: 10.5511/plantbiotechnology.23.1220a

Ngoc-Ha Thi Tran, Duong Van Hoang, Loc Tuong Phan

{"title":"Drought stress induces early flowering and the stress tolerance of offspring in Petunia hybrida.","authors":"Ngoc-Ha Thi Tran, Duong Van Hoang, Loc Tuong Phan","doi":"10.5511/plantbiotechnology.23.1220a","DOIUrl":"10.5511/plantbiotechnology.23.1220a","url":null,"abstract":"Petunia hybrida (Solanaceae) exhibits high sensitivity to water scarcity, especially during flowering. This study investigated changes in the flowering time of P. hybrida in response to water deficit over a 7-week period. Various levels of water stress-i.e., light, moderate, and severe-were imposed on plants grown in a greenhouse, and these were compared to a control group grown alongside. Remarkably, early flowering was observed under severe stress in P. hybrida for the first time, occurring 5.3 days earlier than in the control group. Furthermore, seeds collected from control and treatment plants were then used to assess drought stress memory in offspring. Seedlings were cultivated in a dehydration medium containing either PEG 8000 or a control MS medium. In the PEG 8000 medium, seedlings from parents exposed to moderate and severe drought stresses exhibited higher drought tolerance than those from well-watered conditions. Moreover, they also displayed significantly longer roots, more leaves, and a lower ion leakage rate. Taken together, these findings demonstrated the presence of positive transgenerational effects on progeny. Thus, while parental drought stress during reproduction stage may affect seed quality, it can enhance drought tolerance in the next generation via induction of stress memory.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 1","pages":"53-63"},"PeriodicalIF":1.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High β-carotene accumulation in transgenic eggplant fruits grown under artificial light. 人工光照下生长的转基因茄子果实中的β-胡萝卜素积累量较高。

IF 1.4 4区生物学

Plant Biotechnology Pub Date : 2024-03-25 DOI: 10.5511/plantbiotechnology.23.1129b

Ryohei Yamamoto, Seigo Higuchi, Yuji Iwata, Satomi Takeda, Nozomu Koizumi, Kei-Ichiro Mishiba

{"title":"High β-carotene accumulation in transgenic eggplant fruits grown under artificial light.","authors":"Ryohei Yamamoto, Seigo Higuchi, Yuji Iwata, Satomi Takeda, Nozomu Koizumi, Kei-Ichiro Mishiba","doi":"10.5511/plantbiotechnology.23.1129b","DOIUrl":"10.5511/plantbiotechnology.23.1129b","url":null,"abstract":"Eggplant (Solanum melongena L.) fruits are known to contain few carotenoids such as β-carotene, which are abundant in congener tomato fruits. In a previous study, we introduced a fruit-specific EEF48 gene promoter-driven crtB gene encoding phytoene synthase (PSY) of Erwinia uredovora into eggplant 'Senryo No. 2'. The transgenic plants grown in a greenhouse set fruits that accumulated β-carotene (∼1.67 µg g-1FW) in the T0 and T1 generations. In the present study, we grew T1 and T2 generations of the transgenic eggplant plants in artificial climate chambers to investigate their fruit set and β-carotene accumulation. No clear difference in β-carotene accumulation was observed in the fruit of transgenic plants grown under either HID (high-intensity discharge) or LED (light-emitting diode) light, or between T1 and T2 generations. The β-carotene accumulation (8.83 µg g-1FW on average) was approximately 5 times higher than the previous results obtained from greenhouse-grown plants. However, the fruit weight and size of the T-DNA (+) plants were significantly smaller than that of their null-segregant T-DNA (-) plants derived from the same line, suggesting that β-carotene accumulation may inhibit fruit development. Considering that a part of plants grown under LED irradiation failed to set fruits or set smaller fruits than those grown under HID irradiation, the light condition in the LED chamber may not be sufficient to promote fruit development. The present results are expected to provide valuable information for the selection of transgenic eggplants with high β-carotene content in fruit under artificial lighting.","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 1","pages":"77-81"},"PeriodicalIF":1.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0